Base de dados : LILACS
Pesquisa : G02.111.158 [Categoria DeCS]
Referências encontradas : 54 [refinar]
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Id: lil-788951
Autor: Bhari, Ranjeeta; Kaur, Bhawanpreet; Singh, Ram S.
Título: Lectin activity in mycelial extracts of Fusarium species
Fonte: Braz. j. microbiol;47(3):775-780, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Lectins are non-immunogenic carbohydrate-recognizing proteins that bind to glycoproteins, glycolipids, or polysaccharides with high affinity and exhibit remarkable ability to agglutinate erythrocytes and other cells. In the present study, ten Fusarium species previously not explored for lectins were screened for the presence of lectin activity. Mycelial extracts of F. fujikuroi, F. beomiformii, F. begoniae, F. nisikadoi, F. anthophilum, F. incarnatum, and F. tabacinum manifested agglutination of rabbit erythrocytes. Neuraminidase treatment of rabbit erythrocytes increased lectin titers of F. nisikadoi and F. tabacinum extracts, whereas the protease treatment resulted in a significant decline in agglutination by most of the lectins. Results of hapten inhibition studies demonstrated unique carbohydrate specificity of Fusarium lectins toward O-acetyl sialic acids. Activity of the majority of Fusarium lectins exhibited binding affinity to D-ribose, L-fucose, D-glucose, L-arabinose, D-mannitol, D-galactosamine hydrochloride, D-galacturonic acid, N-acetyl-d-galactosamine, N-acetyl-neuraminic acid, 2-deoxy-D-ribose, fetuin, asialofetuin, and bovine submaxillary mucin. Melibiose and N-glycolyl neuraminic acid did not inhibit the activity of any of the Fusarium lectins. Mycelial extracts of F. begoniae, F. nisikadoi, F. anthophilum, and F. incarnatum interacted with most of the carbohydrates tested. F. fujikuroi and F. anthophilum extracts displayed strong interaction with starch. The expression of lectin activity as a function of culture age was investigated. Most species displayed lectin activity on the 7th day of cultivation, and it varied with progressing of culture age.
Descritores: Micélio
Fusarium/metabolismo
Fusarium/química
Lectinas/metabolismo
-Testes de Hemaglutinação
Eritrócitos/efeitos dos fármacos
Metabolismo dos Carboidratos
Fusarium/crescimento & desenvolvimento
Hemaglutinação
Lectinas/farmacologia
Limites: Seres Humanos
Animais
Responsável: BR1.1 - BIREME


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Teixeira, Maria Francisca Simas
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Id: lil-788950
Autor: Kirsch, Larissa de Souza; Macedo, Ana Júlia Porto de; Teixeira, Maria Francisca Simas.
Título: Production of mycelial biomass by the Amazonian edible mushroom Pleurotus albidus
Fonte: Braz. j. microbiol;47(3):658-664, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Edible mushroom species are considered as an adequate source of food in a healthy diet due to high content of protein, fiber, vitamins, and a variety of minerals. The representatives of Pleurotus genus are characterized by distinct gastronomic, nutritional, and medicinal properties among the edible mushrooms commercialized worldwide. In the present study, the growth of mycelial biomass of Pleurotus albidus cultivated in submerged fermentation was evaluated. Saccharose, fructose, and maltose were the three main carbon sources for mycelial biomass formation with corresponding yields of 7.28 g L−1, 7.07 g L−1, and 6.99 g L−1. Inorganic nitrogen sources did not stimulate growth and the optimal yield was significantly higher with yeast extract (7.98 g L−1). The factorial design used to evaluate the influence of saccharose and yeast extract concentration, agitation speed, and initial pH indicated that all variables significantly influenced the production of biomass, especially the concentration of saccharose. The greater amount of saccharose resulted in the production of significantly more biomass. The highest mycelial biomass production (9.81 g L−1) was reached in the medium formulated with 30.0 g L−1 saccharose, 2.5 g L−1 yeast extract, pH 7.0, and a speed of agitation at 180 rpm. Furthermore, P. albidus manifested different aspects of morphology and physiology under the growth conditions employed. Media composition affected mycelial biomass production indicating that the diversification of carbon sources promoted its improvement and can be used as food or supplement.
Descritores: Biomassa
Pleurotus/fisiologia
Micélio
Fermentação
-Carbono/metabolismo
Pleurotus/ultraestrutura
Metabolismo dos Carboidratos
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


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Ayub, Marco Antonio Zachia
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Id: biblio-828200
Autor: Coghetto, Chaline Caren; Vasconcelos, Carolina Bettker; Brinques, Graziela Brusch; Ayub, Marco Antônio Záchia.
Título: Lactobacillus plantarum BL011 cultivation in industrial isolated soybean protein acid residue
Fonte: Braz. j. microbiol;47(4):941-948, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aeration rate and stirring speed as well as the concentrations of liquid acid protein residue of soybean, soy peptone, corn steep liquor, and raw yeast extract. The results showed that all variables, except for corn steep liquor, significantly influenced biomass production. The best condition was applied to bioreactor cultures, which produced a maximal biomass of 17.87 g L-1, whereas lactic acid, the most important lactic acid bacteria metabolite, peaked at 37.59 g L-1, corresponding to a productivity of 1.46 g L-1 h-1. This is the first report on the use of liquid acid protein residue of soybean medium for L. plantarum growth. These results support the industrial use of this system as an alternative to produce probiotics without animal-derived ingredients to obtain high biomass concentrations in batch bioreactors.
Descritores: Proteínas de Soja
Meios de Cultura
Lactobacillus plantarum/crescimento & desenvolvimento
Lactobacillus plantarum/metabolismo
-Biomassa
Ácido Láctico/biossíntese
Proteínas de Soja/metabolismo
Proteínas de Soja/química
Reatores Biológicos
Meios de Cultura/química
Metabolismo dos Carboidratos
Fermentação
Hidrólise
Responsável: BR1.1 - BIREME


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Id: lil-788971
Autor: Ouf, Salama A; Moussa, Tarek A; Abd-Elmegeed, Alshimaa M; Eltahlawy, Samar R.
Título: Anti-fungal potential of ozone against some dermatophytes
Fonte: Braz. j. microbiol;47(3):697-702, July-Sept. 2016. tab.
Idioma: en.
Resumo: ABSTRACT Dermatophytes are classified in three genera, Epidermophyton, Microsporum and Trichophyton. They have the capacity to invade keratinized tissue to produce a cutaneous infection known as dermatophytoses. This investigation was performed to study the effect of gaseous ozone and ozonized oil on three specific properties of six different dermatophytes. These properties included sporulation, mycelia leakage of sugar and nutrients and the activity of their hydrolytic enzymes. Generally, ozonized oil was found to be more efficacious than gaseous ozone. Microsporum gypseum and Microsporum canis were the most susceptible, while Trichophyton interdigitale and T. mentagrophytes were relatively resistant. The study revealed a steady decline in spore production of M. gypseum and M. canis on application of ozonated oil. An increase in leakage of electrolytes and sugar was noticed after treatment with ozonized oil in the case of M. gypseum, M. canis, T. interdigitale, T. mentagrophytes and T. rubrum. The results also revealed loss in urease, amylase, alkaline phosphatase, lipase and keratinase enzyme producing capacity of the investigated fungi.
Descritores: Ozônio/farmacologia
Arthrodermataceae/efeitos dos fármacos
Antifúngicos/farmacologia
-Permeabilidade
Esporos Fúngicos/efeitos dos fármacos
Proteínas Fúngicas/metabolismo
Micélio
Arthrodermataceae/fisiologia
Eletrólitos/metabolismo
Ativação Enzimática
Metabolismo dos Carboidratos/efeitos dos fármacos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-788963
Autor: Blanco, Alina Sánchez; Durive, Osmar Palacios; Pérez, Sulema Batista; Montes, Zoraida Díaz; Guerra, Nelson Pérez.
Título: Simultaneous production of amylases and proteases by Bacillus subtilis in brewery wastes
Fonte: Braz. j. microbiol;47(3):665-674, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT The simultaneous production of amylase (AA) and protease (PA) activity by Bacillus subtilis UO-01 in brewery wastes was studied by combining the response surface methodology with the kinetic study of the process. The optimum conditions (T = 36.0 °C and pH = 6.8) for high biomass production (0.92 g/L) were similar to the conditions (T = 36.8 °C and pH = 6.6) for high AA synthesis (9.26 EU/mL). However, the maximum PA level (9.77 EU/mL) was obtained at pH 7.1 and 37.8 °C. Under these conditions, a considerably high reduction (between 69.9 and 77.8%) of the initial chemical oxygen demand of the waste was achieved. In verification experiments under the optimized conditions for production of each enzyme, the AA and PA obtained after 15 h of incubation were, respectively, 9.35 and 9.87 EU/mL. By using the Luedeking and Piret model, both enzymes were classified as growth-associated metabolites. Protease production delay seemed to be related to the consumption of non-protein and protein nitrogen. These results indicate that the brewery waste could be successfully used for a high scale production of amylases and proteases at a low cost.
Descritores: Peptídeo Hidrolases/biossíntese
Bacillus subtilis/metabolismo
Fermentação
Amilases/biossíntese
Resíduos Industriais
-Temperatura Ambiente
Cinética
Biotransformação
Metabolismo dos Carboidratos
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME


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Moreira, Renato de Azevedo
Cavada, Benildo Sousa
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Id: lil-623973
Autor: Moreira, Renato de Azevedo; Ainouz, Iracema Lima; Oliveira, José Tadeu Abreu de; Cavada, Benildo Sousa.
Título: Plant lectins, chemical and biological aspects
Fonte: Mem. Inst. Oswaldo Cruz;86(supl.2):211-218, 1991. tab.
Idioma: en.
Conferência: Apresentado em: Brazilian-Sino Symposium on Chemistry and Pharmacology of Natural Products, Rio de Janeiro, Dec. 10-14, 1989.
Resumo: Lectins, carbohydrate-binding proteins of non-immune origin, that agglutinate cells or precipitate polysaccharides and glycoconjugates, are well distributed in nature, mainly in the Plant Kingdom. The great majority of the plante lectins are present in seed cotyledons where they are found in the cytoplasm or int he protein bodies, although they have also been found in roots, stems and leaves. Due to their peculiar properties, the lectins are used as a tool both for analytical and preparative purposes in biochemistry, cellular biology, immunology and related areas. In agriculture and medicine the use of lectins greatly improved in the last few years. The lextins, with few exceptions, are glycoproteins, need divalent cations to display full activity and are, in general, oligomers with variable molecular weight. Although the studies on lectins have completed a century, their role in nature is yet ynknown . Several hypotheses on their physiological functions have been suggested. Thus, lectins could play important roles in defense against pathogens, plant-microorganism symbiosis, cell organization, embryo morphogenesis, phagocytosis, cell wall elongation, pollen recognition and as reserve proteins. A brief review on the general properties and roles of the lectins is given.
Descritores: Proteínas de Plantas/farmacologia
Plantas/química
Lectinas/isolamento & purificação
Lectinas/química
-Proteínas na Dieta/farmacologia
Glicoproteínas/farmacologia
Metabolismo dos Carboidratos
Limites: Seres Humanos
Animais
Responsável: BR1.1 - BIREME


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Id: biblio-895331
Autor: Souza, Taciane M; Lopes, Thiago V; Wajnsztejn, Henry; Pazdiora, Raul D; Riet-Correa, Franklin; Fujihara, Rodrigo I; Mansur, Igor M; Schons, Sandro V.
Título: Timpanismo gastrointestinal em equídeos alimentados com Panicum maximum com alto conteúdo de amido / Intestinal bloat in equids fed with Panicum maximum of high starch content
Fonte: Pesqui. vet. bras = Braz. j. vet. res;37(10):1079-1084, out. 2017. tab, graf.
Idioma: pt.
Projeto: INCT.
Resumo: Timpanismo intestinal agudo em cavalos e mulas introduzidos em pastagens de Panicum maximum cv. Tanzânia, cv. Massai e cv. Mombaça são relatados na região norte do Brasil, durante o período das chuvas. As causas são desconhecidas, porém, tem sido sugerido que estão associados a um maior armazenamento de carboidratos não fibrosos na gramínea. Este trabalho teve por objetivos realizar um levantamento epidemiológico dos surtos de cólicas ocorridos no Estado de Rondônia, assim como contribuir com o estudo da ação dos carboidratos de P. maximum na ocorrência da doença. Para isso foram realizadas visitas em 10 propriedades que apresentaram casos da enfermidade. Em duas propriedades foram introduzidos equinos no mês de março e foram coletadas amostras de pastagens para a determinação de energia digestiva, lisina, matéria seca, carboidratos solúveis em água, carboidratos solúveis em éter, carboidratos não fibrosos, fibra de detergente neutra, fibra de detergente ácido, lignina, amido, gordura e proteína bruta. Essas determinações foram realizadas, também, em amostras de pastagens de P. maximum de outras propriedades onde não estavam ocorrendo cólicas e das pastagens de Cynodon dactylon de onde provinham os equinos. Os resultados mostraram que os surtos de cólica ocorrem na época de chuva, quando as pastagens estão rebrotando com uma morbidade de 31,6%, e letalidade de 86,1%. As análises bromatológicas mostraram uma diferença significativa nas concentrações de amido (P=0,0072) do P. maximum que causou cólica (8,3 % e 9,2%), quando comparado com o P. maximum das outras propriedades (0,9% e 4,3%) e com o Tifton, utilizado previamente na alimentação dos mesmos equinos (0,2% e 0,8%). Em outra fazenda a doença foi reproduzida experimentalmente em um equino, no mês de março administrando P. maximum cortado contendo 6,5% de amido. A mesma pastagem não causou cólica quando foi administrada a equinos entre os meses de novembro a fevereiro, com 2,7% a 5,1% de amido. Estes resultados sugerem que a causa das cólicas em equídeos em pastagens de P. maximum, na época de rebrote, é o alto conteúdo de amido das mesmas.(AU)

Acute intestinal bloat occurs in horses and mules introduced into sprouting Panicum maximum cv. Tanzania, cv. Massai and cv. Mombaça pastures in northern Brazil during the rainy season. It has been suggested that the disease is due to high concentrations of non-structural carbohydrates in this grass. The paper aimed to study outbreaks of colics in the state of Rondônia, Brazil and contribute to the knowledge of the carbohydrates contained in P. maximum as the cause of the disease. For this, ten farms which had cases of intestinal bloat were visited. On two farms, horses were introduced into such a toxic pasture. Samples of pasture were collected for determination of digestible energy, lysine, dry matter, water soluble carbohydrates, ether soluble carbohydrates, non-fiber carbohydrates, neutral detergent fiber, acid detergent fiber, lignin, starch, and fat. Samples of P. maximum pastures from neighboring farms, where the disease was not occurring, and from Cynodon dactylon pastures where the horses had been grazing before were also collected. The results showed that colics occur in the rainy season in sprouting pasture with a morbidity of 31.6%, and lethality of 86.1%. The pastures which induce colic showed significantly (P=0.0072) higher starch concentration (8,3 and 9,2%) than pastures of P. maximum which did not cause colic (0,9 and 4,3%) as well as the Cynodon dactylon pastures ingested previously by the horses (0.2 and 0.8%. On another farm, the disease was produced experimentally in a horse receiving in the feeder P. maximum collected from a toxic pasture with 6.5% of starch. The same pasture did not cause colic from November to February with starch content of 2.7 to 5.1%. It is suggested that the cause of colic in equids ingesting sprouting pasture of P. maximum is the high starch content of this pasture during the groth period.(AU)
Descritores: Amido/toxicidade
Pastagens/efeitos adversos
Cólica
Equidae
Metabolismo dos Carboidratos
Gastroenteropatias/veterinária
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-615037
Autor: Márquez Guillén, Antonio; Lang Prieto, Jacinto; Valdés Amador, Lemay; Cruz Hernández, Jeddú; Guerrero Rodríguez, Ederlis.
Título: Prediabetes y diabetes gestacional / Prediabetes and gestational diabetes
Fonte: Rev. cuba. endocrinol;22(1):58-60, ene.-abr. 2011.
Idioma: es.
Resumo: Se debe considerar a la diabetes gestacional (DG) como toda intolerancia a los carbohidratos que aparece o se reconoce durante el embarazo, independientemente del momento de la gestación al diagnóstico del trastorno metabólico, si se administra o no insulina, o si desaparece o no después del parto. Por ello, resulta un grupo heterogéneo de situaciones con diferentes expectativas y pronósticos, en dependencia del momento de su presentación y del grado de afectación metabólica que manifieste...(AU)
Descritores: Estado Pré-Diabético/epidemiologia
Fatores de Risco
Diabetes Gestacional/diagnóstico
-Metabolismo dos Carboidratos/fisiologia
Limites: Seres Humanos
Feminino
Gravidez
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-615029
Autor: Díaz Díaz, Oscar; Cabrera Rode, Eduardo; Orlandi González, Neraldo; Araña Rosaínz, Manuel de Jesús; Díaz Horta, Oscar.
Título: Aspectos epidemiológicos de la prediabetes, diagnóstico y clasificación / Epidemiological characteristics of prediabetes, diagnosis and classification
Fonte: Rev. cuba. endocrinol;22(1):3-10, ene.-abr. 2011.
Idioma: es.
Resumo: La creciente prevalencia y progresión de la prediabetes a la diabetes (DM) ha convertido a la morbilidad y la mortalidad relacionadas con ella en un problema importante de salud pública. Los diabéticos son vulnerables a complicaciones múltiples y complejas (cardiopatía, accidente cerebrovascular, arteriopatía periférica y microangiopatía entre otras). Las evidencias epidemiológicas indican que esas complicaciones comienzan ya antes de que los pacientes hayan llegado al estadio de diabetes manifiesta. La intervención en los cambios en el estilo de vida sobre las personas en etapas de prediabetes, tiene el potencial de reducir la incidencia de diabetes y la enfermedad cardiovascular y microvascular relacionadas. el término prediabetes se aplica a aquellos casos en los que los niveles de glucemia se encuentran por encima de los valores para individuos normales, pero por debajo de los niveles considerados para DM. Dada su alta frecuencia resulta conveniente considerar la prediabetes como un estado de riesgo importante para la predicción de diabetes y de complicaciones vasculares, así como una manifestación subclínica de un trastorno del metabolismo de los carbohidratos
Descritores: Estado Pré-Diabético/classificação
Estado Pré-Diabético/diagnóstico
Estado Pré-Diabético/epidemiologia
-Diabetes Mellitus/epidemiologia
Metabolismo dos Carboidratos/fisiologia
Limites: Seres Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-1016815
Autor: Nunes Lage, Nara; Macedo Lopes, Juliana Márcia; Pereira, Renata Rebeca; Ferreirra da Costa Guerra, Joyce; Albuquerque Pereira, Mariana de Fátima; Silva, Maisa; De Freitas Bonomo, Larissa; Geraldo de Lima, Wanderson; Eustáquio Silva, Marcelo; Lúcia Pedrosa, Maria.
Título: Antioxidant potential of Buriti (Mauritia flexuosa) pulp flour in diabetic rats
Fonte: Arch. latinoam. nutr;68(1):59-70, mar. 2018. ilus, tab, graf.
Idioma: en.
Resumo: Buriti pulp flour (BPF) contains significant levels of antioxidants. This study evaluated the effect of BPF on biomarkers of oxidative damage in the liver, heart, and pancreas of diabetic rats. The chemical composition, antioxidant capacity, and polyphenol content of BPF were determined. Thirty-six female Fisher rats were divided into four groups: control (C); control + BPF (CB); diabetic (D); diabetic + BPF (DB). Diabetes was induced by treatment with streptozotocin. Thirty days after the induction of diabetes, glucose, total cholesterol and triacylglycerides serum levels, aminotransferase and paraoxonase activities were evaluated. Oxidative damage to lipids and proteins was assessed through thiobarbituric acid reactive substances (TBARS) and protein carbonyl analyses, respectively. Histopathological analyses were also performed. BPF contained high concentrations of phenolic compounds, lipids, and fibers, and exhibited a high capacity to neutralize the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Diabetes was evidenced by equivalent high levels of glucose in plasma from rats in the D and DB groups. Diabetic rats in both groups also presented the same increased activity of aminotransferases. Protein carbonyl levels were increased in liver, heart, and pancreas in the D compared with C group. Although treatment with BPF did not result in any histopathological alterations, it reduced significantly the levels of TBARS in the heart and protein carbonyls in the liver and heart. No effect on blood glucose and tissue histology was observed following treatment with BPF. However, BPF diminished oxidative damage in liver and heart, indicating a possible antioxidant potential in vivo, in addition to in vitro(AU)

La harina de pulpa buriti (BPF) contiene niveles significativos de antioxidantes. Este estudio evaluó el efecto del BPF en biomarcadores de daño oxidativo en el hígado, el corazón y el páncreas de ratas diabéticas. Se determino la composición química, la capacidad antioxidante y el contenido de polifenoles del BPF. Treinta y seis ratas Fisher fueron divididas en cuatro grupos: Control (C); Control + BPF (CB); Diabético (D); Diabético + BPF (DB). La diabetes fue inducida por tratamiento con estreptozotocina. Treinta dias después de la inducción de la diabetes, se evaluaron los niveles séricos de glucosa, colesterol total y triacilglicéridos, y las actividades de aminotransferasa y paraoxonasa. El daño oxidativo a lípidos y proteínas se evaluó a través de sustancias reactivas al ácido tiobarbitúrico (TBARS) y análisis de proteínas carboniladas respectivamente. También se realizaron análisis histopatológicos. El BPF contenía altas concentraciones de compuestos fenólicos, lípidos y fibras, y exhibía una alta capacidad para neutralizar el radical 2,2-difenil-1-picrilhidracil (DPPH). La diabetes se evidenció por altos niveles de glucosa en plasma de ratas en los grupos D y DB. Las ratas diabéticas en ambos grupos también presentaron la misma actividad aumentada de las aminotransferasas. Los niveles de proteínas carboniladas se incrementaron en el hígado, el corazón y el páncreas en el grupo D en comparación con el C. Aunque el tratamiento con BPF no dio lugar a alteraciones histopatológicas, redujo significativamente los niveles de TBARS en el corazón y las proteínas carboniladas en el hígado y el corazón. No se observo ningún efecto sobre la glucosa en la sangre y la histología de tejidos después del tratamiento con BPF. Sin embargo, el BPF disminuyó el daño oxidativo en el hígado y el corazón, lo que indica un posible potencial antioxidante in vivo, además de in vitro(AU)
Descritores: Diabetes Mellitus/etiologia
Metabolismo dos Carboidratos
Hiperglicemia/etiologia
Antioxidantes/análise
-Diabetes Mellitus Experimental
Lipídeos
Limites: Ratos
Tipo de Publ: Estudos de Avaliação
Responsável: VE557.1 - Biblioteca Fundación Bengoa



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