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Pesquisa : G02.111.225 [Categoria DeCS]
Referências encontradas : 32 [refinar]
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Texto completo SciELO Chile
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Id: biblio-950861
Autor: Marthandan, Shiva; Menzel, Uwe; Priebe, Steffen; Groth, Marco; Guthke, Reinhard; Platzer, Matthias; Hemmerich, Peter; Kaether, Christoph; Diekmann, Stephan.
Título: Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
Fonte: Biol. Res;49:1-16, 2016. ilus, graf.
Idioma: en.
Projeto: German Ministry for Education and Research.
Resumo: BACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determined the transcriptional changes combined with the transition into replicative senescence in primary human fibroblast strains. Here, by γ-irradiation we induced premature cellular senescence in the fibroblast cell strains (HFF and MRC-5) and determined the corresponding transcriptional changes by high-throughput RNA sequencing. RESULTS: Comparing the transcriptomes, we found a high degree of similarity in differential gene expression in replicative as well as in irradiation induced senescence for both cell strains suggesting, in each cell strain, a common cellular response to error accumulation. On the functional pathway level, "Cell cycle" was the only pathway commonly down-regulated in replicative and irradiation-induced senescence in both fibroblast strains, confirming the tight link between DNA repair and cell cycle regulation. However, "DNA repair" and "replication" pathways were down-regulated more strongly in fibroblasts undergoing replicative exhaustion. We also retrieved genes and pathways in each of the cell strains specific for irradiation induced senescence. CONCLUSION: We found the pathways associated with "DNA repair" and "replication" less stringently regulated in irradiation induced compared to replicative senescence. The strong regulation of these pathways in replicative senescence highlights the importance of replication errors for its induction.
Descritores: Senescência Celular/fisiologia
Fibroblastos/efeitos da radiação
-Fatores de Tempo
Dano ao DNA
Immunoblotting
Regulação para Baixo/efeitos da radiação
Regulação para Cima/efeitos da radiação
Células Cultivadas
Análise de Variância
Senescência Celular/efeitos da radiação
Senescência Celular/genética
beta-Galactosidase/metabolismo
Análise de Sequência de RNA
Perfilação da Expressão Gênica
Feto Abortado
Reparo do DNA/efeitos da radiação
Replicação do DNA/efeitos da radiação
Fibroblastos/fisiologia
Raios gama
Pulmão
Limites: Humanos
Masculino
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-748272
Autor: Lima, Tainah P.; Farinatti, Paulo T.V.; Rubini, Ercole C.; Silva, Elirez B.; Monteiro, Walace D..
Título: Hemodynamic responses during and after multiple sets of stretching exercises performed with and without the Valsalva maneuver
Fonte: Clinics;70(5):333-338, 05/2015. graf.
Idioma: en.
Resumo: OBJECTIVE: This study investigated the acute hemodynamic responses to multiple sets of passive stretching exercises performed with and without the Valsalva maneuver. METHODS: Fifteen healthy men aged 21 to 29 years with poor flexibility performed stretching protocols comprising 10 sets of maximal passive unilateral hip flexion, sustained for 30 seconds with equal intervals between sets. Protocols without and with the Valsalva maneuver were applied in a random counterbalanced order, separated by 48-hour intervals. Hemodynamic responses were measured by photoplethysmography pre-exercise, during the stretching sets, and post-exercise. RESULTS: The effects of stretching sets on systolic and diastolic blood pressure were cumulative until the fourth set in protocols performed with and without the Valsalva maneuver. The heart rate and rate pressure product increased in both protocols, but no additive effect was observed due to the number of sets. Hemodynamic responses were always higher when stretching was performed with the Valsalva maneuver, causing an additional elevation in the rate pressure product. CONCLUSIONS: Multiple sets of unilateral hip flexion stretching significantly increased blood pressure, heart rate, and rate pressure product values. A cumulative effect of the number of sets occurred only for systolic and diastolic blood pressure, at least in the initial sets of the stretching protocols. The performance of the Valsalva maneuver intensified all hemodynamic responses, which resulted in significant increases in cardiac work during stretching exercises. .
Descritores: Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
Benzodioxóis/farmacologia
Neoplasias do Colo/tratamento farmacológico
Isoquinolinas/farmacologia
Inibidores de Proteínas Quinases/farmacologia
Tiofenos/farmacologia
Inibidores da Topoisomerase I/farmacologia
Ureia/análogos & derivados
-CHECKPOINT KINASE TEMEFOS
Replicação do DNA/efeitos dos fármacos
Sinergismo Farmacológico
HTABORTION, INCOMPLETE CELLS
Proteínas Quinases/metabolismo
Proteínas Serina-Treonina Quinases/metabolismo
Ureia/farmacologia
Limites: Humanos
Tipo de Publ: Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-741446
Autor: Coêlho, Thaís Gonzalez da Silveira; Caracas, Hugo Cesar Pinto Marques.
Título: Perception of the relationship between TMD and orthodontic treatment among orthodontists
Fonte: Dental press j. orthod. (Impr.);20(1):45-51, Jan-Feb/2015. tab, graf.
Idioma: en.
Resumo: INTRODUCTION: The consensus about the relationship between TMD and orthodontic treatment has gone from a cause and effect association between TMD and orthodontic treatment to the idea that there is no reliable evidence supporting this statement. OBJECTIVE: To assess the beliefs, despite scientific evidence, of Brazilian orthodontists about the relationship between TMD and orthodontic treatment with regards to treatment, prevention and etiology of TMD. METHODS: A survey about the relationship between TMD and orthodontic treatment was prepared and sent to Brazilian orthodontists by e-mail and social networks. Answers were treated by means of descriptive statistics and strong associations between variables were assessed by qui-square test. RESULTS: The majority of orthodontists believe that orthodontic treatment not only is not the best treatment option for TMD, but also is not able to prevent TMD. Nevertheless, the majority of orthodontists believe that orthodontic treatment can cause TMD symptoms. CONCLUSION: This study suggests that orthodontists' beliefs about the relationship between orthodontic treatment and TMD are in accordance with scientific evidence only when referring to treatment and prevention of TMD. The majority of orthodontists believe that, despite scientific evidence, orthodontic treatment can cause TMD. .

INTRODUÇÃO: o consenso sobre a relação entre DTM e tratamento ortodôntico foi de uma associação de causa e efeito à ideia de que não há evidências confiáveis que suportem essa afirmação. OBJETIVO: avaliar as crenças, sem considerar as evidências, de ortodontistas brasileiros sobre a relação entre DTM e tratamento ortodôntico com relação ao tratamento, prevenção e etiologia da DTM. MÉTODOS: um questionário sobre a relação entre DTM e tratamento ortodôntico foi preparado e enviado a ortodontistas brasileiros por meio de e-mail e mídias sociais. As respostas foram analisadas por estatística descritiva, e fortes associações entre as variáveis foram verificadas pelo teste χ2. RESULTADOS: a maioria dos ortodontistas acredita que o tratamento ortodôntico não é o melhor tratamento para DTM. Além disso, acreditam que não é a melhor forma para sua prevenção. Também, a maioria dos ortodontistas acredita que o tratamento ortodôntico pode causar sintomas de DTM. CONCLUSÃO: este estudo sugere que as crenças dos ortodontistas sobre a relação entre tratamento ortodôntico e DTM estão de acordo com as evidências científicas apenas quando se trata do tratamento e da prevenção de DTM. A maioria dos ortodontistas acredita que, apesar das evidências científicas, o tratamento ortodôntico pode causar DTM. .
Descritores: Proteínas de Ciclo Celular/metabolismo
Replicação do DNA/genética
Fatores de Transcrição Forkhead/metabolismo
Fase G1/fisiologia
Regulação da Expressão Gênica/genética
Proteínas Serina-Treonina Quinases/metabolismo
Origem de Replicação/genética
Transdução de Sinais/genética
-Western Blotting
Fracionamento Celular
Linhagem Celular
Proteínas de Ciclo Celular/genética
/metabolismo
CYCLIN-DEPENDENT KINASE INHIBITOR PABNORMALITIES, DRUG-INDUCED/metabolismo
Primers do DNA/genética
Imunofluorescência
Fatores de Transcrição Forkhead/genética
Immunoblotting
Imunoprecipitação
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Proteínas Serina-Treonina Quinases/genética
Proteínas Proto-Oncogênicas c-myc/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Interferência de RNA
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Lopes, Ademar
Chammas, Roger
Id: lil-691976
Autor: Bettoni, Fabiana.
Título: Metabolismo do DNA e mutações / DNA metabolism and mutations
Fonte: In: Lopes, Ademar; Chammas, Roger; Iyeyasu, Hirofumi. Oncologia para a graduação. São Paulo, Lemar, 2013. p.30-37. (Oncologia para a graduação).
Idioma: pt.
Descritores: Ácidos Nucleicos/ultraestrutura
DNA
Replicação do DNA
Mutação
-Reparo do DNA
Biossíntese de Proteínas
Responsável: BR30.1 - Biblioteca
BR30.1


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Id: lil-640982
Autor: Gimenes, F; Takeda, K. I; Fiorini, A; Gouveia, F. S; Fernandez, M. A.
Título: Intrinsically bent DNA in replication origins and gene promoters
Fonte: Genet. mol. res. (Online);7(2):549-558, 2008. ilus.
Idioma: en.
Resumo: Intrinsically bent DNA is an alternative conformation of the DNA molecule caused by the presence of dA/dT tracts, 2 to 6 bp long, in a helical turn phase DNA or with multiple intervals of 10 to 11 bp. Other than flexibility, intrinsic bending sites induce DNA curvature in particular chromosome regions such as replication origins and promoters. Intrinsically bent DNA sites are important in initiating DNA replication, and are sometimes found near to regions associated with the nuclear matrix. Many methods have been developed to localize bent sites, for example, circular permutation, computational analysis, and atomic force microscopy. This review discusses intrinsically bent DNA sites associated with replication origins and gene promoter regions in prokaryote and eukaryote cells. We also describe methods for identifying bent DNA sites for circular permutation and computational analysis.
Descritores: DNA
Conformação de Ácido Nucleico
Origem de Replicação/genética
Regiões Promotoras Genéticas/genética
-Biologia Computacional
Simulação por Computador
Células Procarióticas/metabolismo
Genes
Modelos Biológicos
Replicação do DNA/fisiologia
Limites: Humanos
Animais
Responsável: BR26.1 - Biblioteca Central


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Id: lil-613066
Autor: Ostos Ortiz, Olga Lucía.
Título: La molécula de la vida en su dimensión hipercompleja: diálogo entre saberes de sistemas complejos e hipercomplejos: [editoriales]
Fonte: NOVA publ. cient;7(12), jul.-dic. 2009. graf.
Idioma: es.
Descritores: DNA
Replicação do DNA
Biologia de Sistemas
Tipo de Publ: Editorial
Responsável: CO242.1 - Biblioteca


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Texto completo SciELO Brasil
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Id: lil-544615
Autor: Isea, Raúl; Ramírez, José Luis; Hoebeke, Johan.
Título: Assessing protein stability of the dimeric DNA-binding domain of E2 human papillomavirus 18 with molecular dynamics
Fonte: Mem. Inst. Oswaldo Cruz;105(2):123-126, Mar. 2010. ilus.
Idioma: en.
Resumo: The objective of this study is to understand the structural flexibility and curvature of the E2 protein of human papillomavirus type 18 using molecular dynamics (6 ns). E2 is required for viral DNA replication and its disruption could be an anti-viral strategy. E2 is a dimer, with each monomer folding into a stable open-faced â-sandwich. We calculated the mobility of the E2 dimer and found that it was asymmetric. These different mobilities of E2 monomers suggest that drugs or vaccines could be targeted to the interface between the two monomers.
Descritores: DNA Viral/genética
Proteínas de Ligação a DNA/genética
/genética
HUMAN PAPILLOMAVIRUS 1ABDOMINAL NEOPLASMS/genética
Proteínas Oncogênicas Virais/genética
-Dimerização
Replicação do DNA
DNA Viral/metabolismo
Proteínas de Ligação a DNA/metabolismo
/metabolismo
HUMAN PAPILLOMAVIRUS 1ABDOMINAL NEOPLASMS/metabolismo
Modelos Moleculares
Proteínas Oncogênicas Virais/metabolismo
Estabilidade Proteica
Replicação Viral
Responsável: BR1.1 - BIREME


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Id: lil-479685
Autor: Gimenes, F; Gouveia, F. de S; Fiorini, A; Fernandez, M. A.
Título: Intrinsic bent DNA sites in the chromosomal replication origin of Xylella fastidiosa 9a5c
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;41(4):295-304, Apr. 2008. ilus, graf.
Idioma: en.
Projeto: CNPq; . Academy of Science for the Developing World; . CAPES.
Resumo: The features of the nucleotide sequences in both replication and promoter regions have been investigated in many organisms. Intrinsically bent DNA sites associated with transcription have been described in several prokaryotic organisms. The aim of the present study was to investigate intrinsic bent DNA sites in the segment that holds the chromosomal replication origin, oriC, of Xylella fastidiosa 9a5c. Electrophoretic behavior analyses, as well as in silico analyses of both the 2-D projection and helical parameters, were performed. The chromosomal segment analyzed contains the initial sequence of the rpmH gene, an intergenic region, the dnaA gene, the oriC sequence, and the 5' partial sequence of the dnaN gene. The analysis revealed fragments with reduced electrophoretic mobility, which indicates the presence of curved DNA segments. The analysis of the helical parameter ENDS ratio revealed three bent DNA sites (b1, b2, and b3) located in the rpmH-dnaA intergenic region, the dnaA gene, and the oriC 5' end, respectively. The chromosomal segment of X. fastidiosa analyzed here is rich in phased AT tracts and in CAnT motifs. The 2-D projection indicated a segment whose structure was determined by the cumulative effect of all bent DNA sites. Further, the in silico analysis of the three different bacterial oriC sequences indicated similar negative roll and twist >34.00° values. The DnaA box sequences, and other motifs in them, may be associated with the intrinsic DNA curvature.
Descritores: Cromossomos Bacterianos/genética
DNA Bacteriano/genética
Origem de Replicação/genética
Xylella/genética
-Sequência de Bases
Replicação do DNA/genética
Eletroforese em Gel de Ágar
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
Texto completo
Id: lil-468189
Autor: Zimmermann, Katrin; Holtz, Marlis; Phi-Van, Loc.
Título: The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
Fonte: Biol. Res;40(2):185-192, 2007. ilus.
Idioma: en.
Projeto: Deutsche Forschungsgemeinschaft.
Resumo: We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase.
Descritores: Ciclo Celular/genética
Cromatina/genética
Replicação do DNA/genética
Fatores de Transcrição/genética
-Linhagem Celular
Ciclo Celular/fisiologia
Cromatina/química
Cromatina/metabolismo
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-449146
Autor: Chiaratti, M. R; Meirelles, F. V.
Título: Increase in mitochondrial DNA quantity and impairment of oxidative phosphorylation in bovine fibroblast cells treated with ethidium bromide for 15 passages in culture
Fonte: Genet. mol. res. (Online);5(1):55-62, Mar. 31, 2006.
Idioma: en.
Resumo: Bovine fetal fibroblast cells were treated with ethidium bromide at a low concentration for 15 passages in culture to determine its effect on mitochondrial DNA copy number and on cell metabolism. Mitochondrial membrane potential and lactate production were estimated in order to characterize cell metabolism. In addition, mitochondrial DNA ND5 in proportion to a nuclear gene (luteinizing hormone receptor) was determined at the 1st, 2nd, 3rd, 10th, and 15th passages using semi-quantitative PCR amplification. Treated cells showed a lower mitochondrial membrane potential and higher levels of lactate production compared with control cells. However, the mitochondrial DNA/nuclear DNA ratio was higher in treated cells compared with control cells at the 10th and 15th passages. This ratio changed between the 3rd and 10th passages. Despite a clear impairment in mitochondrial function, ethidium bromide treatment did not lead to mitochondrial DNA depletion. It is possible that in response to a lower synthesis of ATP, due to an impairment in oxidative phosphorylation, treated cells develop a mechanism to resist the ethidium bromide effect on mtDNA replication, resulting in an increase in mitochondrial DNA copy number.
Descritores: DNA Mitocondrial/efeitos dos fármacos
Etídio/farmacologia
Fibroblastos/efeitos dos fármacos
Fosforilação Oxidativa/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
-Células Cultivadas
DNA Mitocondrial/metabolismo
Eletroforese em Gel de Poliacrilamida
Feto
Fibroblastos/metabolismo
Replicação do DNA/efeitos dos fármacos
Limites: Animais
Bovinos
Masculino
Responsável: BR1.1 - BIREME



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