Base de dados : LILACS
Pesquisa : G02.111.225.880 [Categoria DeCS]
Referências encontradas : 14 [refinar]
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Id: biblio-889070
Autor: Wang, Jiaqi; Pan, Yixuan; Hu, Jiacheng; Ma, Qiang; Xu, Yi; Zhang, Yijian; Zhang, Fei; Liu, Yingbin.
Título: Tea polyphenols induce S phase arrest and apoptosis in gallbladder cancer cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;51(4):e6891, 2018. graf.
Idioma: en.
Resumo: Gallbladder cancer (GBC) is the most common malignancy in the biliary tract. Without effective treatment, its prognosis is notoriously poor. Tea polyphenols (TPs) have many pharmacological and health benefits, including antioxidant, anti-inflammatory, anti-tumor, anti-thrombotic, antibacterial, and vasodilatory properties. However, the anti-cancer effect of TPs in human gallbladder cancer has not yet been determined. Cell viability and colony formation assay were used to investigate the cell growth. Cell cycle and apoptosis were evaluated by flow cytometry analysis. Western blot assay was used to detect the expression of proteins related to cell cycle and apoptosis. Human tumor xenografts were used to examine the effect of TPs on gallbladder cancer cells in vivo. TPs significantly inhibited cell growth of gallbladder cancer cell lines in a dose- and time-dependent manner. Cell cycle progression in GBC cells was blocked at the S phase by TPs. TPs also induced mitochondrial-related apoptosis in GBC cells by upregulating Bax, cleaved caspase-3, and cleaved PARP expressions and downregulating Bcl-2, cyclin A, and Cdk2 expressions. The effects of TPs on GBC were further proven in vivo in a mouse xenograft model. Our study is the first to report that TPs inhibit GBC cell growth and these compounds may have potential as novel therapeutic agents for treating gallbladder cancer.
Descritores: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Camellia sinensis/química
Neoplasias da Vesícula Biliar/patologia
Polifenóis/farmacologia
Fase S/efeitos dos fármacos
Chá/química
-Antineoplásicos Fitogênicos/isolamento & purificação
Linhagem Celular Tumoral
Proliferação de Células/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Neoplasias da Vesícula Biliar/tratamento farmacológico
Xenoenxertos
Polifenóis/isolamento & purificação
Limites: Humanos
Responsável: BR1.1 - BIREME


  2 / 14 LILACS  
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Id: lil-694711
Autor: XU, Li-na; LU, Bi-nan; HU, Ming-ming; XU, You-wei; HAN, Xu; QI, Yan; PENG, Jin-yong.
Título: Mechanisms involved in the cytotoxic effects of berberine on human colon cancer HCT-8 cells
Fonte: Biocell;36(3):113-120, Dec. 2012. graf.
Idioma: en.
Projeto: Excellent Young Scientist funds; . Science and Technology Foundation of Dalian; . First Special China Post-Doctor Foundation; . Ministry of Education. Academic Scholarship for Doctoral Candidates.
Resumo: Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ethidium bromide staining. The concentrations of lactate dehydrogenase and both acid and alkaline phosphatases were significantly increased in cell supernatants after berberine treatment, suggesting cell death. Furthermore, flow cytometry analysis showed that berberine could arrest HCT-8 cells at S phase in a time-dependent manner. To further investigate the apoptotic molecular mechanism, reverse transcription-polymerase chain reaction (RT-PCR) and western blotting methods were used. The up-regulated mRNA and/or protein expressions of Fas, FasL, TNF-a, caspase-3 and down-regulation of pro-caspase-3 suggest that the death receptor pathway may be involved in the apoptotic pathway induced by berberine. Decrease of Bcl-2 and increase of Bax in mRNA and/or protein expressions showed that the Bcl-2 family proteins were involved in berberine-induced apoptosis. We also found that berberine-induced apoptosis was associated with an up-regulated expressions of p53 and prohibitin (PHB), and decreased vimentin expression. These results suggest that berberine can suppress cell growth and reduce cell survival by arresting the cell-cycle and by inducing apoptosis of HCT-8 cells.
Descritores: Berberina/farmacologia
Neoplasias do Colo/tratamento farmacológico
Neoplasias do Colo/metabolismo
-Apoptose
Berberina/metabolismo
Ciclo Celular
Linhagem Celular Tumoral
Citometria de Fluxo
L-Lactato Desidrogenase/metabolismo
Medicina Tradicional Chinesa
Microscopia de Fluorescência
RNA Mensageiro/metabolismo
Proteínas Repressoras/farmacologia
Fase S
Fatores de Tempo
Sais de Tetrazólio/farmacologia
Tiazóis/farmacologia
/metabolismo
TUMOR SUPPRESSOR PROTEIN PDIPETALONEMA INFECTIONS/metabolismo
Vimentina/metabolismo
/metabolismo
BCL-TEMEFOS-ASSOCIATED X PROTEIN/metabolismo
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


  3 / 14 LILACS  
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Id: lil-595046
Autor: Ryu, Mi Heon; Park, Jeong Hee; Park, Ji Eun; Chung, Jin; Lee, Chang Hun; Park, Hae Ryoun.
Título: Cobalt chloride stimulates phosphoinositide 3-kinase/Akt signaling through the epidermal growth factor receptor in oral squamous cell carcinoma
Fonte: Biocell;34(1):15-21, Apr. 2010. graf.
Idioma: en.
Resumo: Tumor cells are often found under hypoxic conditions due to the rapid outgrowth of their vascular supply, and, in order to survive hypoxia, these cells induce numerous signaling factors. Akt is an important kinase in cell survival, and its activity is regulated by the upstream phosphoinositide 3-kinase (PI3K) and receptor tyrosine kinases (RTKs). In this study, we examined Akt activation and RTKs/PI3K/Akt signaling using the hypoxia-mimetic cobalt chloride in oral squamous carcinoma cells. Cobalt chloride increases Akt phosphorylation in both a dose- and time-dependent manner. Blocking the activation of the PI3K/Akt pathway using LY294002 abolished Akt activation in response to cobalt chloride, suggesting that Akt phosphorylation by cobalt chloride is dependent on PI3K. In addition, activation of the PI3K/Akt path way seems to rely on the epidermal growth factor receptor (EGFR), since the inhibition of EGFR attenuated cobalt chloride-induced Akt activation. The results in this study also demonstrate that cobalt chloride increases EGFR protein levels and induces oral squamous cell carcinoma cells to enter S phase.
Descritores: DNA de Neoplasias/biossíntese
Carcinoma de Células Escamosas/metabolismo
Carcinoma de Células Escamosas/patologia
Cobalto/farmacologia
/metabolismo
FOSFATIDILINOSITOL ABATTOIRS-QUINASA/metabolismo
Hipóxia Celular
Neoplasias Bucais/metabolismo
Neoplasias Bucais/patologia
Proteínas Proto-Oncogênicas c-akt/metabolismo
-Linhagem Celular Tumoral
Fase S
Receptores ErbB/metabolismo
Transdução de Sinais
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


  4 / 14 LILACS  
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Id: lil-456761
Autor: Frigieri, M. C; Thompson, G. M; Pandolfi, J. R; Zanelli, C. F; Valentini, S. R.
Título: Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae
Fonte: Genet. mol. res. (Online);6(1):152-165, 2007. tab, ilus.
Idioma: en.
Resumo: The putative eukaryotic translation initiation factor 5A (eIF5A) is an essential protein for cell viability and the only cellular protein known to contain the unusual amino acid residue hypusine. eIF5A has been implicated in translation initiation, cell proliferation, nucleocytoplasmic transport, mRNA decay, and actin polarization, but the precise biological function of this protein is not clear. However, eIF5A was recently shown to be directly involved with the translational machinery. A screen for synthetic lethal mutations was carried out with one of the temperature-sensitive alleles of TIF51A (tif51A-3) to identify factors that functionally interact with eIF5A and revealed the essential gene YPT1. This gene encodes a small GTPase, a member of the rab family involved with secretion, acting in the vesicular trafficking between endoplasmatic reticulum and the Golgi. Thus, the synthetic lethality between TIF51A and YPT1 may reveal the connection between translation and the polarized distribution of membrane components, suggesting that these proteins work together in the cell to guarantee proper protein synthesis and secretion necessary for correct bud formation during G1/S transition. Future studies will investigate the functional interaction between eIF5A and Ypt1 in order to clarify this involvement of eIF5A with vesicular trafficking.
Descritores: Genes Letais/genética
Mutação/genética
Fatores de Iniciação de Peptídeos/genética
Proteínas de Ligação a RNA/genética
Proteínas de Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/genética
Proteínas rab de Ligação ao GTP/genética
-Fase G1/genética
Fase S/genética
Saccharomyces cerevisiae/citologia
Vesículas Transportadoras/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 14 LILACS  
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Burbano, R. R
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Id: lil-417597
Autor: Lima, P. D. de; Cardoso, P. C; Khayat, A. S; Bahia, M. de O; Burbano, R. R.
Título: Evaluation of the mutagenic activity of hydroxyurea on the G1-S-G2 phases of the cell cycle: an in vitro study
Fonte: Genet. mol. res. (Online);2(3):328-333, Sept. 2003.
Idioma: en.
Resumo: Hydroxyurea is considered an antineoplastic drug, which also plays an important role in the treatment of sickle cell anemia patients. We evaluated and compared the clastogenic and cytotoxic effects of hydroxyurea, using chromosomal aberrations and mitotic index, respectively, as endpoints. In vitro short-term cultures of lymphocytes were exposed to several concentrations of this drug, at various cell cycle phases. There was a significant increase in the cytotoxicity of hydroxyurea at G1 and G1/S as well in the G2 phase of the cell cycle. Hydroxyurea did not significantly increase chromosome aberrations. There was an S-dependent cytotoxic effect of hydroxyurea, which is expected based on the known activity of hydroxyurea as an inhibitor of ribonucleotide reductase
Descritores: Aberrações Cromossômicas/induzido quimicamente
Antineoplásicos/toxicidade
Hidroxiureia/toxicidade
Interfase/efeitos dos fármacos
Linfócitos/efeitos dos fármacos
-Análise de Variância
Determinação de Ponto Final
Fase G1/efeitos dos fármacos
Fase G1/genética
/efeitos dos fármacos
FASE GTEMEFOS/efeitos dos fármacos
/genética
FASE GTEMEFOS/genética
Fase S/efeitos dos fármacos
Fase S/genética
Interfase/genética
Índice Mitótico
Testes de Mutagenicidade/métodos
Limites: Humanos
Tipo de Publ: Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME


  6 / 14 LILACS  
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Id: lil-410592
Autor: Freitas Júnior, Ruffo de; Souza, Sandro Ângelo de; Sousa, Rosemar Macedo; Pereira, Rubens José; Freitas, Nilceana Maya Aires.
Título: Citometria de fluxo utilizando-se aspirados da PAAF nos tumores da mama / Flow citometry using FNAC aspirates in breast tumors
Fonte: Rev. bras. mastologia;13(1):19-24, jan.-mar. 2003. tab, graf.
Idioma: pt.
Resumo: A citometria de fluxo(CF)tem sido utilizada como fator prognóstico para o câncer de mama; entretanto a confiabilidade do método utilizando-se aspirados obtidos de punção aspirativa por agulha fina(PAAF)permanece controversa. Objetivo: Comparar o coeficiente de variação(CV), o índice de DNA(DNA), a fase de síntese(fase S)e a aneuploidia utilizando-se aspirados provenientes de PAAF com o tecido histológico de peças cirúrgicas arquivado em parafina. Material e métodos: Foram utilizados 44 peças frescas contendo tumores mamários. Em cada peça ex vivo, procedeu-se a duas punções por agulha fina. Posteriormente as peças foram fixadas e incluídas em parafina. Tanto os aspirados quanto o material histológico, desparafinizado, foram preparados para a citometria de fluxo. O exame foi feito utilizando-se um aparelho BD FACScan, com leitura de 10 mil células por exame. Resultados: A média do CV para os aspirados foi de 6,05(+ ou - 1,60)e da peça foi de 6,96(+ ou - 2,13)(p < 0,05). A média do índice de DNA foi de 1,6(+ ou - 0,5)para os aspirados e de 1,49(+ ou - 0,42)para as peças cirúrgicas(NS). A média da fase-S foi de 7,2(+ ou - 6,34) e de 5,85(+ ou - 4,73)para a citologia e para o material histológico, respectivamente (NS). Quanto à aneuploidia, a média nos aspirados foi de 38,2(+ ou -24,7) e de 29,8(+ ou - 20)para a peça cirúrgica(NS). Conclusão: Os aspirados da PAAF são bastante confiáveis para a realização da citometria de fluxo, podendo ser ainda melhores que o material desparafinizado
Descritores: Aneuploidia
Biópsia por Agulha Fina/métodos
Neoplasias da Mama
DNA de Neoplasias
Citometria de Fluxo
Prognóstico
Fase S
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR14.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: lil-356879
Autor: Pelayo, Helvia R; Pincheira, Juana; Gimenez-Abian, Juan F; Clarke, Duncan J; Torre, Consuelo de la.
Título: p53-independent checkpoint controls in a plant cell model
Fonte: Biol. Res;36(3/4):381-388, 2003. ilus, tab, graf.
Idioma: en.
Resumo: Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous override, cells became accumulated in G2-prophase, giving rise later on to a delayed mitotic wave. Cell growth was maintained during the checkpoint blocks, as the delayed mitoses were larger in size than the control ones. Under continuous hydroxyurea treatment, the delayed mitotic was formed by two subpopulations: normal mitoses corresponding to cells having properly recovered from the checkpoint block, and abnormal ones resulting from checkpoint adaptation. These latter cells displayed broken chromatids as they had unduly overriden the G2 checkpoint block, without completing DNA repair. The frequency of the checkpoint-adapted mitoses increased with the hydroxyurea concentration from 0.25 to 1.0 mM. However, from 1 mM hydroxyurea upwards, some of the cells lost their competence for checkpoint adaptation. Therefore, the dose of a genotoxic agent that still allows G2 checkpoint adaptation should always be applied in order to get rid of uncontrolled proliferating cells. This is specially suitable for cells lacking a functional p53 protein.
Descritores: Inibidores Enzimáticos
Hidroxiureia
Cebolas
Proteína Supressora de Tumor p53
-Ciclo Celular
Citometria de Fluxo
Fase G2
Genes cdc
Cebolas
Fase S
Responsável: BR1.1 - BIREME


  8 / 14 LILACS  
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Id: lil-340382
Autor: Paglini, M. G; Rovasio, R. A.
Título: Fibronectin substrate induces shortening of in vitro neural crest cell mitotic cycle
Fonte: Biocell;23(2):119-124, Aug. 1999.
Idioma: en.
Resumo: Neural crest cells (NCC) segregate from neural folds, swim through extracellular matrix, and differentiate in several cell types. During in vivo and in vitro dispersion, NCC display a substantial proliferative rate. Here, we have evaluated this biological behavior under the influence of different substrates. NCC exhibit the highest proliferative activity on fibronectin substrate, with shortening of the G1 period. This fact is reverted by specific antibody pre-treatment of the substrate. Taking into account that NCC migrate along narrow and restricted pathways rich in essential fibronectin, with high proliferative rates, these results indicate that the fibronectin pathway contributes to increase the cell number, leading to a high population pressure that could participate in the early dispersion of NCC
Descritores: Células Cultivadas
Fibronectinas
Fase G1
Fase G2
Crista Neural
Fase S
Limites: Animais
Embrião de Galinha
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


  9 / 14 LILACS  
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Id: lil-272134
Autor: Zveibil, Deborah Krutman.
Título: Avaliação do conteúdo de DNA em carcinomas invasivos da mama por meio de análise de imagem: interação de fatores prognósticos citométricos com a avaliação morfológica e imuno-histoquímica.
Fonte: São Paulo; s.n; 1995. 187 p. tab.
Idioma: pt.
Tese: Apresentada a Universidade Federal de Säo Paulo. Escola Paulista de Medicina para obtenção do grau de Doutor.
Descritores: Mama
Imuno-Histoquímica
Ploidias
Fase S
Responsável: BR1.2 - Biblioteca Central
BR1.2; 2125


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Id: lil-211964
Autor: Cao Pochintesta, Carmen.
Título: Fase S e índice de ADN en cáncer mamario / S status and DNA index in breast cancer
Fonte: Rev. Méd. Clín. Condes;8(3):84-6, dic. 1997. ilus, tab.
Idioma: es.
Descritores: Neoplasias da Mama/genética
Marcadores Genéticos
Fase S/genética
-Inclusão em Parafina
Aneuploidia
Biópsia por Agulha/estatística & dados numéricos
Biópsia/estatística & dados numéricos
Sobreviventes/estatística & dados numéricos
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central



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