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Pesquisa : G02.111.263 [Categoria DeCS]
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Id: biblio-1278433
Autor: Gul, Anum; Siddiqui, Maryam; Arain, Habiba; Khan, Sadaf; Khan, Hanzala; Ishrat, Urooj.
Título: Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste
Fonte: Braz. arch. biol. technol;64:e21200639, 2021. tab, graf.
Idioma: en.
Projeto: Higher Education Commission.
Resumo: Abstract Ananas Comosus (also known as pineapple) is a part of Bromeliaceae family and it is consumed as food as well as folk medicine for the treatment of various diseases. It is reported that pineapple is a rich source of bromelain, a cysteine protease and it is considered as an important enzyme in different industries due to its significant therapeutic and industrial applications such as anticancer, anti-inflammatory and meat tenderizing. Bromelain is mostly present in fruit and stem of pineapple, but it is reported that crown, core, and peels, which constitute the waste of the pineapple plant, also contain bromelain but limited data is available. Therefore, the proposed study aimed at utilizing pineapple waste for the extraction and characterization of bromelain. Firstly, crude bromelain was extracted with phosphate buffer (pH 7), then it was subjected to partial purification using different fractions of ammonium sulphate (NH4)2SO4 such as 30, 40, 50 and 60% followed by desalting and concentration. Enzyme activity was calculated by using casein digesting unit (CDU) method. The results demonstrated that the crown bromelain showed highest purification of 4.34-fold at 30% (NH4)2SO4 saturation, whereas core and peel bromelain showed highest purification of 2.75 and 2.59-fold at 40% (NH4)2SO4 saturation. The molecular weight of crude and partially purified bromelain was determined by SDS-PAGE analysis and found to be 26 KDa. The pH and thermal stability of all the parts of pineapple showed maximum stability at pH 7 and at 35oC temperature.
Descritores: Bromelaínas/isolamento & purificação
Ativação Enzimática
Sulfato de Amônio
-Peptídeo Hidrolases
Eletroforese em Gel de Poliacrilamida
Responsável: BR1.1 - BIREME


  2 / 340 LILACS  
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Id: biblio-1278442
Autor: Luo, Hao; Shen, Tie; Xie, Xiaoyao.
Título: The Relationship between Functional and Evolutionary Correlations of Enzyme Reactions in Metabolic Network Evolution
Fonte: Braz. arch. biol. technol;64:e21190480, 2021. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Collaborative Innovation Center of Guizhou Educational Committee; . Science and Technology Foundation of Guizhou Province; . Science and Technology Cooperation Program of Guizhou Province.
Resumo: Abstract The evolution of species is inevitably accompanied by the evolution of metabolic networks to adapt to different environments. The metabolic networks of different species were collected from the Kyoto Encyclopedia of Genes and Genomes (KEGG) website, and some enzyme reactions with the highest occurrence frequency in all species were found and are reported in this paper. The correlation coefficients of whether the enzyme reactions appear in all species were calculated, and the corresponding evolutionary correlation connection networks were calculated according to different correlation coefficient thresholds. These studies show that, as the evolutionary correlation of enzyme reactions increases, the weighted average of the mean functional concentration ratios of the enzyme reactions also increases, indicating that the functional concentration ratio of enzyme reactions has a certain correlation with the evolutionary correlation. The work presented in this paper enhances our understanding of the characteristics and general rules of metabolic network evolution.
Descritores: Ativação Enzimática
Redes e Vias Metabólicas
-Adaptação Biológica
Metabolismo
Responsável: BR1.1 - BIREME


  3 / 340 LILACS  
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Id: biblio-1278447
Autor: Ozcelebi, Halime; Ari, Ferda; Dere, Egemen.
Título: Glutathione S-Transferase Activity in Tissues of Rats Exposed to Fenarimol
Fonte: Braz. arch. biol. technol;64:e21200751, 2021. graf.
Idioma: en.
Projeto: Bursa Uludag University.
Resumo: Abstract The unconscious use of pesticides causes various adverse effects on non-target organisms, including humans. Enzymes that control metabolism become the target of the pesticide and the organs are damaged due to toxic effects. Glutathione s-transferase (GST, EC 2.5.1.18), an important enzyme of the detoxification mechanism and antioxidant defense system, can be affected by such toxic substances. Therefore, the effect of fenarimol on GST enzyme activity was investigated in our study. For this, 200 mg/kg fenarimol was administered intraperitoneally to male and female rats at different periods (2, 4, 8, 16, 32, 64 and 72 hours). After application, GST enzyme activity was analysed in the liver, kidney, brain and small intestine tissues of the rats. According to our results, activation (liver, kidney, small intestine) or inhibition (brain) of the generally GST enzyme was observed in the tissues of rats exposed to fenarimol. It is thought that the increase and/or decrease in this enzyme activity may be the cause of the toxic effect of fenarimol.
Descritores: Praguicidas/efeitos adversos
Glutationa S-Transferase pi
-Ativação Enzimática
Fungicidas Industriais/efeitos adversos
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


  4 / 340 LILACS  
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Id: biblio-950716
Autor: Hung, Tran Manh; Dang, Nguyen Hai; Dat, Nguyen Tien.
Título: Methanol extract from Vietnamese Caesalpinia sappan induces apoptosis in HeLa cells
Fonte: Biol. Res;47:1-5, 2014. ilus, graf, tab.
Idioma: en.
Projeto: Vietnam National Foundation for Science and Technology Development.
Resumo: BACKGROUND: This study evaluated the cytotoxic activity of extracts from Caesalpinia sappan heartwood against multiple cancer cell lines using an MTT cell viability assay. The cell death though induction of apoptosis was as indicated by DNA fragmentation and caspase-3 enzyme activation. RESULTS: A methanol extract from C. sappan (MECS) showed cytotoxic activity against several of the cancer cell lines. The most potent activity exhibited by the MECS was against HeLa cells with an IC50 value of 26.5 ± 3.2 µg/mL. Treatment of HeLa cells with various MECS concentrations resulted in growth inhibition and induction of apoptosis, as indicated by DNA fragmentation and caspase-3 enzyme activation. CONCLUSION: This study is the first report of the anticancer properties of the heartwood of C. sappan native to Vietnam. Our findings demonstrate that C. sappan heartwood may have beneficial applications in the field of anticancer drug discovery.
Descritores: Extratos Vegetais/farmacologia
Apoptose
Caesalpinia/química
Feixe Vascular de Plantas/metabolismo
Antineoplásicos Fitogênicos/farmacologia
-Sais de Tetrazólio
Vietnã
Ensaios de Seleção de Medicamentos Antitumorais/métodos
Células HeLa
Sobrevivência Celular
Concentração Inibidora 50
Citotoxinas/farmacologia
Linhagem Celular Tumoral
Proliferação de Células/efeitos dos fármacos
Metanol
Ativação Enzimática/efeitos dos fármacos
Caspase 3/metabolismo
Fragmentação do DNA
Formazans
Inibidores do Crescimento/farmacologia
Indicadores e Reagentes
Camundongos Endogâmicos C57BL
Antineoplásicos Fitogênicos/isolamento & purificação
Limites: Humanos
Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  5 / 340 LILACS  
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Id: biblio-1132156
Autor: Monteiro, Mônica Cristina Pereira; Tavares, Dérica Gonçalves; Nery, Eduardo Mateus; Queiroz, Marisa Vieira de; Pereira, Olinto Liparini; Cardoso, Patrícia Gomes.
Título: Enzyme Production by Induratia spp. Isolated from Coffee Plants in Brazil
Fonte: Braz. arch. biol. technol;63:e20180673, 2020. tab.
Idioma: en.
Projeto: Foundation of Research Support of Minas Gerais State.
Resumo: Abstract Endophytic fungi belonging to the genus Muscodor now transferred to Induratia are known producers of bioactive volatile organic compounds (VOCs) with many industrial applications. However, the members of this genus have rarely been reported to produce non-volatile metabolites including enzyme. Enzymes of the endophytes are degraders of the polysaccharides available in the host plants and the knowledge of enzyme production by Induratia spp. may provide insights into their possible biotechnological applications. The aim of this study was to evaluate the activity of amylase, cellulase, lipase, pectinase, phytase, protease, endo β-1,4 glucanase and exo β-1,4 glucanase enzymes produced by fungi of the species Induratia coffeana, Induratia yucatanensis and Induratia sp. isolated from organic coffee plants. All Induratia spp. were able to produce the extracellular enzymes cellulase, pectinase, protease, and phytase. Eight fungi were able to produce lipase and four produced amylase. The specific activity of endo β-1, 4 glucanase and exo β-1,4 glucanase enzymes were detected for 9 and 8 endophytic fungi, respectively. This work demonstrated for the first time, the array of enzymes produced by Induratia spp. isolated from Coffea arabica in organic systems in Brazil.
Descritores: Coffea/microbiologia
Ativação Enzimática
Compostos Orgânicos Voláteis/metabolismo
Endófitos/enzimologia
-Brasil
Responsável: BR1.1 - BIREME


  6 / 340 LILACS  
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Id: biblio-950802
Autor: Yoon, Jung Mi; Koppula, Sushruta; Huh, Se Jong; Hur, Sun Jin; Kim, Chan Gil.
Título: Low concentrations of doxycycline attenuates FasL-induced apoptosis in HeLa cells
Fonte: Biol. Res;48:1-9, 2015. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Doxycycline (DC) has been shown to possess non-antibiotic properties including Fas/Fas Ligand (FasL)-mediated apoptosis against several tumor types in the concentration range of 10-40 µg/mL. However, the effect of DC in apoptotic signaling at much low concentrations was not studied. METHODS: The present study investigated the attenuation effect of low dose of DC on FasL-induced apoptosis in HeLa cell by the methods of MTT assay, fluorescence microscopy, DNA fragmentation, flow cytometry analysis, and western blotting. RESULTS AND CONCLUSION: In the present findings we showed that low concentration of DC (<2.0 µg/mL) exhibited protective effects against FasL-induced apoptosis in HeLa cells. FasL treatment to HeLa cells resulted in a concentration-dependent induction of cell death, and treatment with low concentrations of DC (0.1-2 µg/mL) significantly (p < 0.001) attenuated the FasL-induced cell death as measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazo-lium bromide (MTT) assay. Further, the FasL-induced apoptotic features in HeLa cells, such as morphological changes, DNA fragmentation and cell cycle arrest was also inhibited by DC (0.5 µg/mL). Tetracycline and minocycline also showed similar anti-apoptotic effects but were not significant when compared to DC, tested at same concentrations. Further, DC (0.01-16 µg/mL) did not influence the hydrogen peroxide- or cisplatin-induced intrinsic apoptotic pathway in HeLa cells. Protein analysis using Western blotting confirmed that FasL-induced cleavage/activation of cas-pase-8 and caspase-3, were inhibited by DC treatment at low concentration (0.5 µg/mL). Considering the overall data, we report for the first time that DC exhibited anti-apoptotic effects at low concentrations in HeLa cells by inhibition of caspase activation via FasL-induced extrinsic pathway.
Descritores: Apoptose/efeitos dos fármacos
Doxiciclina/administração & dosagem
Caspases/efeitos dos fármacos
Proteína Ligante Fas/efeitos dos fármacos
-Células HeLa
Western Blotting
Doxiciclina/farmacologia
Células NIH 3T3
Relação Dose-Resposta a Droga
Ativação Enzimática
Citometria de Fluxo
Limites: Humanos
Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


  7 / 340 LILACS  
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Id: biblio-950810
Autor: Singh, Thangjam Davis; Meitei, Heikrujam Thoihen; Sharma, Adhikarimayum Lakhikumar; Robinson, Asem; Singh, Lisam Shanjukumar; Singh, Thiyam Ramsing.
Título: Anticancer properties and enhancement of therapeutic potential of cisplatin by leaf extract of Zanthoxylum armatum DC
Fonte: Biol. Res;48:1-9, 2015. ilus, graf, tab.
Idioma: en.
Projeto: Government of India. Department of Biotechnology.
Resumo: BACKGROUND: Clinical use of chemotherapeutic drug, cisplatin is limited by its toxicity and drug resistance. Therefore, efforts continue for the discovery of novel combination therapies with cisplatin, to increase efficacy and reduce its toxicity. Here, we screened 16 medicinal plant extracts from Northeast part of India and found that leaf extract of Zanthoxylum armatum DC. (ZALE) induced cytotoxicity as well as an effect on the increasing of the efficiency of chemotherapeutic drugs (cisplatin, mitomycin C and camptothecin). This work shows detail molecular mechanism of anti-cancer activity of ZALE and its potential for combined treatment regimens to enhance the apoptotic response of chemotherapeutic drugs. RESULTS: ZALE induced cytotoxicity, nuclear blebbing and DNA fragmentation in HeLA cells suggesting apoptosis induction in human cervical cell line. However, the apoptosis induced was independent of caspase 3 activation and poly ADP ribose polymerase (PARP) cleavage. Further, ZALE activated Mitogen-activated protein kinases (MAPK) pathway as revealed by increased phosphorylation of extracellular-signal-regulated kinases (ERK), p38 and c-Jun N-ter-minal kinase (JNK). Inhibition of ERK activation but not p38 or JNK completely blocked the ZALE induced apoptosis suggesting an ERK dependent apoptosis. Moreover, ZALE generated DNA double strand breaks as suggested by the induction γH2AX foci formation. Interestingly, pretreatment of certain cancer cell lines with ZALE, sensitized the cancer cells to cisplatin and other chemotherapeutic drugs. Enhanced caspase activation was observed in the synergistic interaction among chemotherapeutic drugs and ZALE. CONCLUSION: Purification and identification of the bio-active molecules from the ZALE or as a complementary treatment for a sequential treatment of ZALE with chemotherapeutic drugs might be a new challenger to open a new therapeutic window for the novel anti-cancer treatment.
Descritores: Extratos Vegetais/farmacologia
Cisplatino/farmacologia
Zanthoxylum/química
Antineoplásicos Fitogênicos/farmacologia
-Células HeLa
Apoptose/efeitos dos fármacos
Proteínas Quinases Ativadas por Mitógeno/efeitos dos fármacos
Proteínas Quinases JNK Ativadas por Mitógeno/efeitos dos fármacos
Ativação Enzimática/efeitos dos fármacos
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  8 / 340 LILACS  
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Id: biblio-950875
Autor: Cheng, Ming-Jun; Cao, Yun-Gui.
Título: TMPYP4 exerted antitumor effects in human cervical cancer cells through activation of p38 mitogen-activated protein kinase
Fonte: Biol. Res;50:24, 2017. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Chongqing Science and Technology.
Resumo: BACKGROUND: The aim of the present study was to investigate the potential effects of the 5,10,15,20-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP4) on the proliferation and apoptosis of human cervical cancer cells and the underlying mechanisms by which TMPyP4 exerted its actions. RESULTS: After human cervical cancer cells were treated with different doses of TMPyP4, cell viability was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method, the apoptosis was observed by flow cytometry (FCM), and the expression of p38 mitogen-activated protein kinase (MAPK), phosphated p38 MAPK (p-p38 MAPK), capase-3, MAPKAPK2 (MK-2) and poly ADP-ribose polymerase (PARP) was measured by Western blot analysis. The analysis revealed that TMPyP4 potently suppressed cell viability and induced the apoptosis of human cervical cancer cells in a dose-dependent manner. In addition, the up-regulation of p-p38 MAPK expression levels was detected in TMPyP4-treated human cervical cancer cells. However, followed by the block of p38 MAPK signaling pathway using the inhibitor SB203580, the effects of TMPyP4 on proliferation and apoptosis of human cervical cancer cells were significantly changed. CONCLUSIONS: It was indicated that TMPyP4-inhibited proliferation and -induced apoptosis in human cervical cancer cells was accompanied by activating the p38 MAPK signaling pathway. Taken together, our study demonstrates that TMPyP4 may represent a potential therapeutic method for the treatment of cervical carcinoma.
Descritores: Porfirinas/farmacologia
Neoplasias do Colo do Útero/tratamento farmacológico
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
Antineoplásicos/farmacologia
-Sais de Tetrazólio
Células HeLa/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Western Blotting
Reprodutibilidade dos Testes
Apoptose/efeitos dos fármacos
Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores
Proliferação de Células/efeitos dos fármacos
Ativação Enzimática/efeitos dos fármacos
Caspase 3/análise
Formazans
Limites: Humanos
Feminino
Tipo de Publ: Estudo de Avaliação
Responsável: CL1.1 - Biblioteca Central


  9 / 340 LILACS  
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Id: biblio-827863
Autor: Simplicio, Janaina A; Hipólito, Ulisses Vilela; Vale, Gabriel Tavares do; Callera, Glaucia Elena; Pereira, Camila André; Touyz, Rhian M; Tostes, Rita de Cássia; Tirapelli, Carlos R.
Título: Acute Ethanol Intake Induces NAD(P)H Oxidase Activation and Rhoa Translocation in Resistance Arteries / O consumo agudo de etanol induz a ativação da NAD(P)H oxidase e translocação da RhoA em artérias de resistência
Fonte: Arq. bras. cardiol;107(5):427-436, Nov. 2016. graf.
Idioma: en.
Resumo: Abstract Background: The mechanism underlying the vascular dysfunction induced by ethanol is not totally understood. Identification of biochemical/molecular mechanisms that could explain such effects is warranted. Objective: To investigate whether acute ethanol intake activates the vascular RhoA/Rho kinase pathway in resistance arteries and the role of NAD(P)H oxidase-derived reactive oxygen species (ROS) on such response. We also evaluated the requirement of p47phox translocation for ethanol-induced NAD(P)H oxidase activation. Methods: Male Wistar rats were orally treated with ethanol (1g/kg, p.o. gavage) or water (control). Some rats were treated with vitamin C (250 mg/kg, p.o. gavage, 5 days) before administration of water or ethanol. The mesenteric arterial bed (MAB) was collected 30 min after ethanol administration. Results: Vitamin C prevented ethanol-induced increase in superoxide anion (O2-) generation and lipoperoxidation in the MAB. Catalase and superoxide dismutase activities and the reduced glutathione, nitrate and hydrogen peroxide (H2O2) levels were not affected by ethanol. Vitamin C and 4-methylpyrazole prevented the increase on O2- generation induced by ethanol in cultured MAB vascular smooth muscle cells. Ethanol had no effect on phosphorylation levels of protein kinase B (Akt) and eNOS (Ser1177 or Thr495 residues) or MAB vascular reactivity. Vitamin C prevented ethanol-induced increase in the membrane: cytosol fraction ratio of p47phox and RhoA expression in the rat MAB. Conclusion: Acute ethanol intake induces activation of the RhoA/Rho kinase pathway by a mechanism that involves ROS generation. In resistance arteries, ethanol activates NAD(P)H oxidase by inducing p47phox translocation by a redox-sensitive mechanism.

Resumo Fundamento: O mecanismo da disfunção vascular induzido pelo consumo de etanol não é totalmente compreendido. Justifica-se, assim a identificação de mecanismos bioquímicos e moleculares que poderiam explicar tais efeitos. Objetivos: Investigar se a ingestão aguda de etanol ativa a via vascular RhoA/Rho quinase em artérias de resistência e o papel das espécies reativas de oxigênio (ERO) derivadas da NAD(P)H oxidase nessa resposta. Nós também avaliamos se ocorreu translocação da p47phox e ativação da NAD(P)H oxidase após o consumo agudo de etanol. Métodos: Ratos Wistar machos foram tratados com etanol via oral (1g/kg, p.o. gavagem) ou água (controle). Alguns ratos foram tratados com vitamina C (250 mg/kg, p.o. gavagem, 5 dias) antes de água ou etanol. O leito arterial mesentérico (LAM) foi coleado 30 min após a administração de etanol. Resultados: A vitamina C preveniu o aumento da geração de ânion superóxido (O2 -) e lipoperoxidação no LAM induzidos pelo etanol. A atividade da catalase (CAT), da superóxido dismutase (SOD) e os níveis de glutationa reduzida(GSH), nitrato e peróxido de hidrogênio (H2O2) não foram afetados após a ingestão aguda de etanol. A vitamina C e o 4-metilpirazol preveniram o aumento na geração de O2 - induzido pelo etanol em cultura de células do músculo liso vascular (CMLV). O etanol não afetou a fosforilação da proteína quinase B (Akt) e nem da óxido nítrico sintase endotelial (eNOS) (nos resíduos de Ser1177 ou Thr495) ou a reatividade vascular do LAM. A vitamina C preveniu o aumento da razão membrana:citosol da p47phox e a expressão da RhoA no LAM de rato induzido pelo etanol. Conclusão: A ingestão aguda de etanol induz a ativação da via RhoA/Rho quinase por um mecanismo que envolve a geração de ERO. Nas artérias de resistência, o etanol ativa NAD(P)H oxidase induzindo a translocação da p47phox por um mecanismo redox-sensível.
Descritores: Ácido Ascórbico/farmacologia
Estresse Oxidativo/efeitos dos fármacos
NADPH Oxidases/metabolismo
Proteína rhoA de Ligação ao GTP/metabolismo
Etanol/administração & dosagem
Antioxidantes/farmacologia
-Ácido Ascórbico/metabolismo
Ratos Wistar
NADPH Oxidases/efeitos dos fármacos
Transporte Proteico
Modelos Animais de Doenças
Ativação Enzimática
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


  10 / 340 LILACS  
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Id: biblio-886908
Autor: SUFIATE, BRUNA L; SOARES, FILIPPE E F; GOUVEIA, ANGÉLICA S; MOREIRA, SAMARA S; CARDOSO, EVANDRO F; TAVARES, GABRIELLA P; BRAGA, FABIO R; ARAÚJO, JACKSON V DE; QUEIROZ, JOSÉ H DE.
Título: Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice
Fonte: An. acad. bras. ciênc;90(1):461-470, Mar. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice. The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design. The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes. The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated. It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5. The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment.
Descritores: Modelos Estatísticos
Saccharum/metabolismo
Dextranase/biossíntese
Hypocreales/enzimologia
-Temperatura
Dextranos/metabolismo
Meios de Cultura/metabolismo
Eletroforese em Gel de Poliacrilamida
Ativação Enzimática
Sucos de Frutas e Vegetais/análise
Fracionamento Químico/métodos
Concentração de Íons de Hidrogênio
Nitratos
Responsável: BR1.1 - BIREME



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