Base de dados : LILACS
Pesquisa : G02.111.263 [Categoria DeCS]
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Id: biblio-828186
Autor: Ellepola, Arjuna N. B; Samaranayake, L. P; Khan, Z. U.
Título: Extracellular phospholipase production of oral Candida albicans isolates from smokers, diabetics, asthmatics, denture wearers and healthy individuals following brief exposure to polyene, echinocandin and azole antimycotics
Fonte: Braz. j. microbiol;47(4):911-916, Oct.-Dec. 2016. tab.
Idioma: en.
Projeto: Kuwait University Research Grant.
Resumo: Abstract Objective Candida albicans is the primary causative agent of oral candidosis, and one of its key virulent attributes is considered to be its ability to produce extracellular phospholipases that facilitate cellular invasion. Oral candidosis can be treated with polyenes, and azoles, and the more recently introduced echinocandins. However, once administered, the intraoral concentration of these drugs tend to be sub-therapeutic and rather transient due to factors such as the diluent effect of saliva and cleansing effect of the oral musculature. Hence, intra-orally, the pathogenic yeasts may undergo a brief exposure to antifungal drugs. We, therefore, evaluated the phospholipase production of oral C. albicans isolates following brief exposure to sub-therapeutic concentrations of the foregoing antifungals. Materials and methods Fifty C. albicans oral isolates obtained from smokers, diabetics, asthmatics using steroid inhalers, partial denture wearers and healthy individuals were exposed to sub-therapeutic concentrations of nystatin, amphotericin B, caspofungin, ketoconazole and fluconazole for one hour. Thereafter the drugs were removed and the phospholipase production was determined by a plate assay using an egg yolk-agar medium. Results The phospholipase production of these isolates was significantly suppressed with a percentage reduction of 10.65, 12.14, 11.45 and 6.40% following exposure to nystatin, amphotericin B, caspofungin and ketoconazole, respectively. This suppression was not significant following exposure to fluconazole. Conclusions Despite the sub-therapeutic, intra oral, bioavailability of polyenes, echinocandins and ketoconazole, they are likely to produce a persistent antifungal effect by suppressing phospholipase production, which is a key virulent attribute of this common pathogenic yeast.
Descritores: Fosfolipases/biossíntese
Candida albicans/efeitos dos fármacos
Candida albicans/metabolismo
Candidíase Bucal/microbiologia
Candidíase Bucal/tratamento farmacológico
Antifúngicos/farmacologia
-Polienos/uso terapêutico
Polienos/farmacologia
Azóis/uso terapêutico
Azóis/farmacologia
Candida albicans/isolamento & purificação
Candida albicans/patogenicidade
Fumar
Testes de Sensibilidade Microbiana
Dentaduras
Fatores de Virulência
Diabetes Mellitus
Ativação Enzimática
Espaço Extracelular
Equinocandinas/farmacologia
Antifúngicos/uso terapêutico
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-788949
Autor: Peralta, Guillermo Hugo; Bergamini, Carina Viviana; Hynes, Erica Rut.
Título: Aminotransferase and glutamate dehydrogenase activities in lactobacilli and streptococci
Fonte: Braz. j. microbiol;47(3):741-748, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Aminotransferases and glutamate dehydrogenase are two main types of enzymes involved in the initial steps of amino acid catabolism, which plays a key role in the cheese flavor development. In the present work, glutamate dehydrogenase and aminotransferase activities were screened in twenty one strains of lactic acid bacteria of dairy interest, either cheese-isolated or commercial starters, including fifteen mesophilic lactobacilli, four thermophilic lactobacilli, and two streptococci. The strains of Streptococcus thermophilus showed the highest glutamate dehydrogenase activity, which was significantly elevated compared with the lactobacilli. Aspartate aminotransferase prevailed in most strains tested, while the levels and specificity of other aminotransferases were highly strain- and species-dependent. The knowledge of enzymatic profiles of these starter and cheese-isolated cultures is helpful in proposing appropriate combinations of strains for improved or increased cheese flavor.
Descritores: Streptococcus/enzimologia
Glutamato Desidrogenase/metabolismo
Transaminases/metabolismo
Lactobacillus/enzimologia
-Sistema Livre de Células
Ativação Enzimática
Microbiologia de Alimentos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-828205
Autor: Aliabadi, Nasrin; Aminzadeh, Saeed; Karkhane, Ali Asghar; Haghbeen, Kamahldin.
Título: Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
Fonte: Braz. j. microbiol;47(4):931-940, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: International Foundation for Science; . Committee on Scientific and Technological Cooperation; . National Institute of Genetic Engineering and Biotechnology.
Resumo: Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and temperature of incubation were the most significant factors influencing chitinase production. From the tested values, the best pH/temperature was obtained at pH 5 and 70 °C, with Km and V max values of chitinase to be 5.6 mg/mL and 0.87 µmol/min, respectively. Ag+, Co2+, iodoacetamide, and iodoacetic acid inhibited the enzyme activity, whereas Mn2+, Cu2+, Tweens (20 and 80), Triton X-100, and EDTA increased the same. In addition, the study of the morphological alteration of chitin treated by enzyme by SEM revealed cracks and pores on the chitin surface, indicating a potential application of this enzyme in several industries.
Descritores: Bacillus/metabolismo
Quitinases/metabolismo
-Fósforo/metabolismo
Temperatura Ambiente
Bacillus/isolamento & purificação
Bacillus/genética
Bacillus/ultraestrutura
Estabilidade Enzimática/efeitos dos fármacos
Carbono/metabolismo
RNA Ribossômico 16S/genética
Cinética
Quitinases/química
Análise de Sequência de DNA
Ativação Enzimática
Concentração de Íons de Hidrogênio
Íons
Metais
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


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Id: lil-788974
Autor: Gururaj, P; Ramalingam, Subramanian; Devi, Ganesan Nandhini; Gautam, Pennathur.
Título: Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
Fonte: Braz. j. microbiol;47(3):647-657, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: Department of Biotechnology, Government of India.
Resumo: ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a 0.5% (v/v) inoculum, 2% (v/v) castor oil (inducer), and agitation 150 rpm. The optimized conditions from the shake flask experiments were validated in a 3 L lab scale bioreactor, and the lipase production increased to 48 U/mL. The enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography and the overall yield was 36%. SDS-PAGE indicated a molecular weight of 45 kDa for the purified protein, and Matrix assisted laser desorption/ionization time of flight analysis of the purified lipase showed sequence similarity with GDSL family of lipases. The optimum temperature and pH for activity of the enzyme was found to be 50 ºC and 8.0, respectively. The lipase was completely inhibited by phenylmethylsulfonyl fluoride but minimal inhibition was observed when incubated with ethylenediaminetetraacetic acid and dithiothreitol. The enzyme was stable in the presence of non-polar hydrophobic solvents. Detergents like SDS inhibited enzyme activity; however, there was minimal loss of enzyme activity when incubated with hydrogen peroxide, Tween 80 and Triton X-100. The kinetic constants (Km and Vmax) revealed that the hydrolytic activity of the lipase was specific to moderate chain fatty acid esters. The Vmax, Km and Vmax/Km ratio of the enzyme were 16.98 U/mg, 0.51 mM, and 33.29, respectively when 4-nitrophenyl palmitate was used as a substrate.
Descritores: Compostos Orgânicos
Solventes
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/biossíntese
Acinetobacter/enzimologia
Lipase/isolamento & purificação
Lipase/biossíntese
-Compostos Orgânicos/química
Solventes/química
Especificidade por Substrato
Temperatura Ambiente
Proteínas de Bactérias/química
Estabilidade Enzimática
Cinética
Cromatografia por Troca Iônica
Ativação Enzimática
Espaço Extracelular/enzimologia
Concentração de Íons de Hidrogênio
Íons
Lipase/química
Lipólise
Metais
Peso Molecular
Responsável: BR1.1 - BIREME


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Id: lil-788971
Autor: Ouf, Salama A; Moussa, Tarek A; Abd-Elmegeed, Alshimaa M; Eltahlawy, Samar R.
Título: Anti-fungal potential of ozone against some dermatophytes
Fonte: Braz. j. microbiol;47(3):697-702, July-Sept. 2016. tab.
Idioma: en.
Resumo: ABSTRACT Dermatophytes are classified in three genera, Epidermophyton, Microsporum and Trichophyton. They have the capacity to invade keratinized tissue to produce a cutaneous infection known as dermatophytoses. This investigation was performed to study the effect of gaseous ozone and ozonized oil on three specific properties of six different dermatophytes. These properties included sporulation, mycelia leakage of sugar and nutrients and the activity of their hydrolytic enzymes. Generally, ozonized oil was found to be more efficacious than gaseous ozone. Microsporum gypseum and Microsporum canis were the most susceptible, while Trichophyton interdigitale and T. mentagrophytes were relatively resistant. The study revealed a steady decline in spore production of M. gypseum and M. canis on application of ozonated oil. An increase in leakage of electrolytes and sugar was noticed after treatment with ozonized oil in the case of M. gypseum, M. canis, T. interdigitale, T. mentagrophytes and T. rubrum. The results also revealed loss in urease, amylase, alkaline phosphatase, lipase and keratinase enzyme producing capacity of the investigated fungi.
Descritores: Ozônio/farmacologia
Arthrodermataceae/efeitos dos fármacos
Antifúngicos/farmacologia
-Permeabilidade
Esporos Fúngicos/efeitos dos fármacos
Proteínas Fúngicas/metabolismo
Micélio
Arthrodermataceae/fisiologia
Eletrólitos/metabolismo
Ativação Enzimática
Metabolismo dos Carboidratos/efeitos dos fármacos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-788964
Autor: Corrêa, Juliana Moço; Christi, Divair; Torre, Carla Lieko Della; Henn, Caroline; Conceição-Silva, José Luis da; Kadowaki, Marina Kimiko; Simão, Rita de Cássia Garcia.
Título: High levels of ß-xylosidase in Thermomyces lanuginosus: potential use for saccharification
Fonte: Braz. j. microbiol;47(3):680-690, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: CAPES; . Araucária Foundation; . National Council for Scientific and Technological Development.
Resumo: ABSTRACT A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of β-xylosidases activity was evaluated in residual corn straw, and improved so that the optimum condition achieved high β-xylosidases activities 1003 U/mL. According our known, this study is the first to show so high levels of β-xylosidases activities induction. In addition, the application of an experimental design with this microorganism to induce β-xylosidases has not been reported until the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 °C showing better thermostability at 55 °C. The saccharification ability of β-xylosidase in the presence of hemicellulose obtained from corn straw raw and xylan from beechwood substrates showed a xylo-oligosaccharide to xylose conversion yield of 80 and 50%, respectively, at 50 °C. Our data strongly indicated that the β-xylosidases activities was not subjected to the effects of potential enzyme inhibitors often produced during fermentation process. These data suggest the application of this enzyme studied for saccharification of hemicellulose, an abundant residue in the American continents, thus providing an interesting alternative for future tests for energy production.
Descritores: Ascomicetos/enzimologia
Xilosidases/metabolismo
Fermentação
-Polissacarídeos/metabolismo
Polissacarídeos/química
Especificidade por Substrato
Temperatura Ambiente
Xilose/metabolismo
Biomassa
Zea mays/química
Ativação Enzimática
Concentração de Íons de Hidrogênio
Hidrólise
Responsável: BR1.1 - BIREME


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Id: biblio-839339
Autor: Wanderley, Maria Carolina de Albuquerque; Duarte Neto, José Manoel Wanderley; Lima Filho, José Luiz de; Lima, Carolina de Albuquerque; Teixeira, José António Couto; Porto, Ana Lúcia Figueiredo.
Título: Collagenolytic enzymes produced by fungi: a systematic review
Fonte: Braz. j. microbiol;48(1):13-24, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenase) AND (fungi OR fungus OR fungal) AND (production OR synthesis OR synthesize) AND (characterization). Scientific criteria were adopted in this review to classify found articles by score (from 0 to 10). After exclusion criteria, 21 articles were selected. None obtained the maximum of 10 points defined by the methodology, which indicates a deficiency in studies dealing simultaneously with production, characterization and purification of collagenase by fungi. Among microorganisms studied the non-pathogenic fungi Penicillium aurantiogriseum and Rhizoctonia solani stood out in volumetric and specific collagenase activity. The only article found that made sequencing of a true collagenase showed 100% homology with several metalloproteinases fungi. A clear gap in literature about collagenase production by fungi was verified, which prevents further development in the area and increases the need for further studies, particularly full characterization of fungal collagenases with high specificity to collagen.
Descritores: Colágeno/metabolismo
Colagenases/metabolismo
Fungos/metabolismo
-Especificidade por Substrato
Colágeno/química
Colagenases/isolamento & purificação
Colagenases/biossíntese
Colagenases/química
Meios de Cultura
Ativação Enzimática
Proteólise
Fungos/classificação
Responsável: BR1.1 - BIREME


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Id: biblio-837712
Autor: Gao, Wenwei; Zhao, Bo; Liu, Lian; Yuan, Quan; Wu, Xiaojing; Xia, Zhongyuan.
Título: Myocardial ischemic post-conditioning protects the lung against myocardial ischemia/reperfusion-induced damage by activating GSK-3
Fonte: Acta cir. bras;32(5):376-387, May 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Natural Science Foundation of Hubei Province.
Resumo: Abstract Purpose: To investigate whether modulating GSK-3β could attenuate myocardial ischemia reperfusion injury (MIRI) induced acute lung injury (ALI) and analyze the underlying mechanism. Methods: Male SD rats were subjected to MIRI with or without myocardial ischemic post-conditioning in the presence or absence of GSK-3β inhibitor. GSK-3β inhibitor was injected peritoneally 10min before MIRI. Lung W/D weight ratio, MPO, PMNs, histopathological changes, TUNEL, Bax, Bcl-2, IL-6, IL-8, IL-10, GSK-3β, and caspase-3 were evaluated in the lung tissues of all rats. Results: After MIRI, lung injury was significantly increased manifested as significant morphological changes and increased leukocytes in the interstitial capillaries, Lung W/D ratio, MPO, and PMN in BALF, which was associated with enhanced inflammation evidenced by increased expressions of IL-6, IL-8 and reduced expression of IL-10. MIRI significantly increased cell apoptosis in the lung as increased levels of apoptotosis, Bax, cleaved caspase-3, and reduced expression of Bcl-2 was observed, which was concomitant with reduced p-GSK-3β. All these changes were reversed/prevented by ischemic post-conditioning, while these beneficial effects of ischemic post-conditioning were abolished by GSK-3β inhibition. Conclusion: Myocardial ischemia reperfusion injury induces acute lung injury by induction of inflammation and cell apoptosis. Ischemic post-conditioning protects the lung from ALI following MIRI by increasing p-GSK-3β.
Descritores: Traumatismo por Reperfusão Miocárdica/prevenção & controle
Substâncias Protetoras/metabolismo
Lesão Pulmonar Aguda/prevenção & controle
Pós-Condicionamento Isquêmico/métodos
Glicogênio Sintase Quinase 3 beta/metabolismo
-Distribuição Aleatória
Regulação para Baixo
Interleucinas/metabolismo
Ratos Sprague-Dawley
Apoptose/efeitos dos fármacos
Peroxidase/metabolismo
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Substâncias Protetoras/farmacologia
Marcação In Situ das Extremidades Cortadas
Modelos Animais
Ativação Enzimática
Proteína X Associada a bcl-2/metabolismo
Caspase 3/metabolismo
Lesão Pulmonar Aguda/enzimologia
Glicogênio Sintase Quinase 3 beta/antagonistas & inibidores
Glicogênio Sintase Quinase 3 beta/farmacologia
Inflamação/metabolismo
Infarto do Miocárdio/patologia
Neutrófilos/enzimologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-731217
Autor: Novaretti, Marcia Cristina Zago; Santos, Edzangela de Vasconcelos; Quitério, Ligia Maria; Daud-Gallotti, Renata Mahfuz.
Título: Sobrecarga de trabalho da Enfermagem e incidentes e eventos adversos em pacientes internados em UTI / Nursing workload and occurrence of incidents and adverse events in ICU patients / Sobrecarga de trabajo de Enfermería y los incidentes y eventos adversos en pacientes hospitalizados en la UCI
Fonte: Rev. bras. enferm;67(5):692-699, Sep-Oct/2014. tab.
Idioma: pt.
Projeto: Fapesp.
Resumo: Estudo prospectivo, tipo coorte que visou identificar a influência da sobrecarga de trabalho da Enfermagem na ocorrência de incidentes sem lesão e eventos adversos em 399 pacientes internados em Unidades de Terapia Intensiva (UTI). Para coleta de dados, foi aplicado um questionário estruturado e feita análise de prontuários. Nessas admissões, aproximadamente 78% dos incidentes sem lesão e eventos adversos em pacientes foram relacionados à esfera da Enfermagem. Essas ocorrências foram atribuídas à sobrecarga de trabalho, aumentaram o número de dias de internação e o risco de óbito dos pacientes estudados. É fundamental que os gerentes de enfermagem atuem no processo de gestão de pessoas no âmbito hospitalar, evitando a sobrecarga de trabalho e proporcionando, consequentemente, aumento da segurança do paciente.

This prospective cohort study aimed to identify the influence of nursing work overload on the occurrence of incidents without injury and adverse events in 399 patients hospitalized in Intensive Care Units (ICU). For data collection, a structured questionnaire was administered and an analysis of medical records was performed. In these admissions, approximately 78% of incidents without injury and adverse events in patients were related to the sphere of Nursing. These occurrences were attributed to overwork, increased the number of days of hospitalization and the risk of death of patients. It is essential that nursing managers work on the staff hospital management avoiding work overload to contribute for patient safety.

Estudio de cohorte prospectivo que tuvo como objetivo identificar la influencia de la carga de trabajo de enfermería en la ocurrencia de incidentes sin lesión y los eventos adversos en 399 pacientes ingresados en Unidades de Cuidados Intensivos (UCI). Para la recolección de datos, se administró un cuestionario estructurado y realizó el análisis de los registros en prontuarios. En estos ingresos, aproximadamente el 78% de los incidentes sin lesión y los eventos adversos en los pacientes estaban relacionados con el ámbito de la Enfermería. Estos hechos fueron atribuidos al exceso de trabajo, aumentaran el número de días de hospitalización y el riesgo de muerte de los pacientes. Es esencial que los responsables de enfermería que actúan en el proceso de gestión de las personas en los hospitales, eviten el exceso de trabajo y por lo tanto proporcionar una mayor seguridad de los pacientes.
Descritores: Medicamentos de Ervas Chinesas/farmacologia
Hepatócitos/efeitos dos fármacos
-Apoptose/efeitos dos fármacos
CASPASE ABATTOIRS
Caspases/metabolismo
Divisão Celular/efeitos dos fármacos
Linhagem Celular
Ativação Enzimática/efeitos dos fármacos
Hepatócitos/citologia
Cirrose Hepática/tratamento farmacológico
Fitoterapia
Salvia miltiorrhiza
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1002712
Autor: Frías-Quintana, Carlos Alfonso; Álvarez-González, Carlos Alfonso; Guerrero-Zárate, Rocío; Valverde-Chavarría, Silvia; Ulloa-Rojas, Juan B.
Título: Changes in digestive enzymes activities during the initial ontogeny of wolf cichlid, Parachromis dovii (Perciformes: Cichlidae)
Fonte: Neotrop. ichthyol;17(1):e180161, 2019. graf.
Idioma: en.
Projeto: SIA.
Resumo: Wolf cichlid, Parachromis dovii, is a species with a high potential for aquaculture in Central America; however, the knowledge of the digestive physiology in larvae period is limited. For these reason, this study evaluated the changes on digestive enzymes (alkaline and acid proteases, trypsin, chymotrypsin, aminopeptidase, carboxypeptidase, lipases, amylases, and phosphatases) during early ontogeny by biochemical analysis. All digestive enzymes were detected at first feeding (6 days after hatching, DAH, 9.49 mm, 168 degree-days DD). Afterwards all enzymes reached two main peaks in activity at 14 or 22 DAH (15.10 mm, 364 DD and 20.83 mm, 550 DD, respectively). Later, there was a gradual decrease in activity for trypsin and acid and alkaline phosphatases until reach the lowest values at 41 DAH. In the case of acid proteases, chymotrypsin, aminopeptidase, carboxypeptidase, lipase and amylase, all activities reached their maximum values at the end of the larval period, except for alkaline proteases, which showed the maximum value at 14 DAH (15.10 mm, 364 DD). Parachromis dovii larvae have an early capability to hydrolyze exogenous food, agreeing with other carnivorous neotropical cichlid species, for this reason we proposed that the weaning process could begin at 14 DAH.(AU)

El guapote lagunero (Parachromis dovii) es una especie con un alto potencial para la acuicultura en la región de América Central; sin embargo, existe un conocimiento limitado sobre la capacidad digestiva en el periodo larval. Por este motivo, este estudio evaluó los cambios de las enzimas digestivas (proteasas alcalinas y ácidas, tripsina, quimotripsina, aminopeptidasa, carboxipeptidasa, lipasas, amilasas y fosfatasas) durante la ontogenia temprana mediante análisis bioquímico. Todas las enzimas digestivas analizadas se detectaron en la primera alimentación (6 días después de la eclosión, DAH, 9.49 mm, 168 día-grados DD). Después, todas las enzimas alcanzaron dos picos máximos a los 14 o 22 DAH (15.10 mm, 364 DD and 20.83 mm, 550 DD, respectivamente). Después las actividades tripsina, fosfatasas ácidas y alcalina disminuyeron a sus valores más bajos a los 41 DAH. En el caso de las proteasas ácidas y alcalinas, quimotripsina, aminopeptidasa, carboxipeptidasa, lipasa y amilasa, los niveles de actividad aumentaron y alcanzaron su máximo valor al final del período larvario, excepto las proteasas alcalinas, que mostraron su máximo valor a los 14 DAH (15.10 mm, 364 DD). Las larvas de P. dovii tienen una capacidad temprana para hidrolizar alimentos exógenos, lo que concuerda con otras especies de cíclidos neotropicales carnívoros, por lo que proponemos que el proceso de destete inicie a los 14 DAH.(AU)
Descritores: Peptídeo Hidrolases/síntese química
Ciclídeos/fisiologia
Ativação Enzimática
-Aquicultura
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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