Base de dados : LILACS
Pesquisa : G02.111.263 [Categoria DeCS]
Referências encontradas : 331 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 34 ir para página                         

  1 / 331 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-886908
Autor: SUFIATE, BRUNA L; SOARES, FILIPPE E F; GOUVEIA, ANGÉLICA S; MOREIRA, SAMARA S; CARDOSO, EVANDRO F; TAVARES, GABRIELLA P; BRAGA, FABIO R; ARAÚJO, JACKSON V DE; QUEIROZ, JOSÉ H DE.
Título: Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice
Fonte: An. acad. bras. ciênc;90(1):461-470, Mar. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice. The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design. The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes. The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated. It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5. The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment.
Descritores: Modelos Estatísticos
Saccharum/metabolismo
Dextranase/biossíntese
Hypocreales/enzimologia
-Temperatura
Dextranos/metabolismo
Meios de Cultura/metabolismo
Eletroforese em Gel de Poliacrilamida
Ativação Enzimática
Sucos de Frutas e Vegetais/análise
Fracionamento Químico/métodos
Concentração de Íons de Hidrogênio
Nitratos
Responsável: BR1.1 - BIREME


  2 / 331 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-609560
Autor: Suárez, Silvia; Oré, Raquel; Arnao, Inés; Rojas, Luis; Trabucco, Juan.
Título: Extracto acuoso de lepidium meyenii Walp (maca) y su papel como adaptógeno, en un modelo animal de resistencia física / Aquous lepidium meyenii Walp (maca) extract and its role as an adaptogen, in an endurance animal model
Fonte: An. Fac. Med. (Perú);70(3):181-185, jul.-set. 2009. graf.
Idioma: es.
Resumo: Introducción: El Lepidium meyenii Walp (maca) es una raíz andina del Perú, utilizada como alimento por su valor nutricional y propiedades etnomedicinales; es parte de la medicina tradicional. Objetivos: Evaluar el papel de adaptógeno del extracto acuoso de maca amarilla sobre las enzimas del tejido muscular en un modelo animal de resistencia física y estrés oxidativo. Diseño: Experimental. Institución: Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, UNMSM, Lima, Perú. Material biológico: Extracto acuoso de la maca amarilla y ratas macho recién destetados. Intervenciones: Administración del extracto acuoso de la maca amarilla en ratas macho recién destetados, distribuidos en cuatro grupos: (I), de control, (II) 0,4 mg de maca/g de peso (III) 0,8 mg de maca/g de peso y (IV), 1,2 mg de maca/g de peso. El extracto acuoso fue administrado por cánula orogástrica. Se realizó un control de los pesos. Se aplicó la prueba de nado forzado después de 30 días de tratamiento. Los animales fueron sacrificados y se preparó homogenizado de músculo al 10 por ciento. Principales medidas de resultados: Actividad de las enzimas superóxido dismutasa (SOD), catalasa (CAT) y lactato deshidrogenasa (LDH); y, como indicador de proceso oxidativo, se midió la peroxidación lipídica (TBARS). Resultados: El rendimiento en la prueba de resistencia fue la siguiente: (I) 7,09 min; (II) 11,25 min; (III) 11,27 min; (IV) 12,71 min, respectivamente. Las actividades enzimáticas de los grupos I, II, III y IV fueron: SOD (U/mL) 36,6, 18,2, 17,2 y 18,2; CAT (U/L) 18,6, 16,5, 13,4 y 10,3; y LDH (U/mL) 11,6, 6,5, 6,0 y 5,8. TBARS (umol/g tejido): 5,82, 7,15, 4,11 y 4,06. Conclusiones: La administración del extracto acuoso de maca amarilla favorece la respuesta del organismo a una situación estresante y físicamente extenuante, lo que correspondería al papel de un adaptógeno.

Introduction: Lepidium meyenii Walp (maca) is a Peruvian Andean root used as food due to its nutritional value and ethnomedical properties, being part of traditional medicine. Objectives: To determine yellow maca aqueous extract adaptogen role on muscle tissue enzymes in an endurance and oxidative stress animal model. Design: Experimental. Setting: Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material: Yellow maca aqueous extract and newly weaned male rats. Interventions: Administration of yellow maca aqueous extract in newly weaned male rats divided into four groups: (I) control, (II) 0,4 mg maca/g weight, (III) 0,8 mg maca/g, and (IV) 1,2 mg maca/g. The aqueous extract was administered by orogastric cannula. Weights were controlled. Swimming test was applied after 30 days of treatment. The animals were sacrificed and 10 per cent homogenized muscle was prepared. Main outcome measures: Superoxide dismutase (SOD), catalase (CAT) and lactate dehydrogenase (LDH) enzymes activity; lipid peroxidation (TBARS) was measured as an indicator of the oxidative process. Results: The endurance test performance was respectively: (I) 7,09 min; (II) 11,25 min; (III) 11,27 min; (IV) 12,71 min. Groups I, II, III and IV respective enzymatic activities were: SOD (U/mL) 36,6, 18,2, 17,2 and 18,2; CAT (U/L) 18,6, 16,5, 13,4 and 10,3; and LDH (U/mL) 11,6, 6,5, 6,0 and 5,8. TBARS (umol/g tissue): 5,82, 7,15, 4,11 and 4,06. Conclusions: Yellow maca aqueous extract administration helped body response facing a physically exhausting stressful situation, most probably corresponding to an adaptogen role.
Descritores: Ativação Enzimática
Estresse Oxidativo
Extratos Vegetais
Lepidium
Peroxidação de Lipídeos
Resistência Física
-Epidemiologia Experimental
Limites: Animais
Masculino
Ratos
Responsável: PE13.1 - Oficina de Biblioteca, Hemeroteca y Centro de Documentación


  3 / 331 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1115217
Autor: Samacá Martín, Jhoan; Ayala Fajardo, Adis; Uribe Ardila, Alfredo.
Título: Método microespectrofotométrico para la determinación de valores de referencia de la fosfomanosa isomerasa / A Microspectrophotometric Method for the Determination of Reference Values of Phosphomannose Isomerase / Método microespectrofotométrico para determinar valores de referência da fosfomanose isomerase
Fonte: Rev. MED;27(1):29-43, ene.-jun. 2019. tab, graf.
Idioma: es.
Resumo: Resumen: Los desórdenes congénitos de glicosilación son un conjunto de defectos genéticos de tipo multisistémico que afectan la función de la proteína. Se han descrito cerca de 75 enfermedades desde sus primeros estudios. En el presente estudio se desarrolló un método microespectrofotométrico para el diagnóstico de la enzima citosólica fosfomanosa isomerasa EC 5.3.1.8 (PMI), se analizaron 32 muestras de individuos con rango de edad de 0,6 a 27 años y se estableció el intervalo y el valor de referencia de actividad enzimática específica. Este estudio permitirá iniciar el diagnóstico de pacientes deficientes de la PMI de forma temprana y oportuna, lo cual la convierte en una posible enzima candidata para pruebas de tamizaje neonatal, ya que esta patología tiene un tratamiento fácil y de bajo costo, que consiste en la suplementación de manosa en forma oral. El diagnóstico clínico de este desorden metabólico beneficiará al paciente y a su familia al mejorar su calidad de vida, como también al sistema de salud colombiano.

Abstract: Congenital glycosylation disorders are a set of multi-systemic genetic defects affecting protein function. About 75 diseases have been described since early studies. This study developed a microspectrophotometric method for the diagnosis of the cytosolic enzyme phosphomannose isomerase (PMI) (EC 5.3.1.8), analyzed 32 samples of individuals ranging between 0.6 and 27 years old, and established the interval and reference value of specific enzyme activity. This study will allow early and timely diagnosis of PMI deficient patients, which makes this enzyme a potential candidate for neonatal screening tests since this pathology has an easy, low-cost treatment (oral administration of mannose supplements). Clinical diagnosis of this metabolic disorder will benefit the patient and his family by improving his quality of life, as well as the Colombian healthcare system.

Resumo: Os defeitos congénitos de glicosilação são um conjunto de defeitos genéticos de tipo mul-tissistêmico que afetam a função da proteína. Foram descritas 75 doenças desde seus primeiros estudos. Neste estudo, foi desenvolvido um método microespectrofotométrico para diagnosticar a enzima citosólica fosfomanose isomerase EC 5.3.1.8 (PMI); foram analisadas 32 amostras de indivíduos entre 0,6 e 27 anos e estabelecidos o intervalo e o valor de referência de atividade enzimática específica. Este estudo permitirá iniciar o diagnóstico de pacientes deficientes da PMI de forma precoce e oportuna, o que a converte em uma possível enzima candidata para testes genéticos de rastreio pré-natal, já que essa patologia tem um tratamento fácil e de baixo custo, que consiste na suplementação de manose por via oral. O diagnóstico clínico desse defeito metabólico beneficiará o paciente e sua família ao melhorar a qualidade de vida e o sistema de saúde colombiano.
Descritores: Microespectrofotometria
Manose-6-Fosfato Isomerase
-Defeitos Congênitos da Glicosilação
Diagnóstico
Ativação Enzimática
Limites: Humanos
Lactente
Pré-Escolar
Criança
Adolescente
Adulto Jovem
Tipo de Publ: Artigo Clássico
Responsável: CO87.1 - Biblioteca Médica


  4 / 331 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Mancini, Dalva Assunçäo Portari
Mendonça, Ronaldo Zucatelli
Texto completo
Id: lil-417086
Autor: Mancini, Dalva Assunção Portari; Mendonça, Rita Maria Zucatelli; Dias, Andrea Luppi Fernandes; Mendonça, Ronaldo Zucatelli; Pinto, José Ricardo.
Título: Co-infection between influenza virus and flagellated bacteria
Fonte: Rev. Inst. Med. Trop. Säo Paulo;47(5):275-280, Sept.-Oct. 2005.
Idioma: en.
Resumo: Tripsina é necessária na ativação da clivagem do vírus influenza A in vitro. Esta clivagem é importante para entrada do vírus na célula por endocitose mediada pelo receptor celular. Bactérias presentes no trato respiratório são fontes de proteases que podem contribuir na replicação do vírus influenza in vivo. Entre 47 amostras coletadas de cavalos, suínos e humanos, a influenza foi isolada e confirmada em 13 que estavam co-infectadas com bactéria flagelada: Stenotrophomonas maltophilia desde o início destes experimentos. Apesar do tratamento das amostras com antibióticos, as bactérias resistiram em diversas delas (48.39%). A protease (elastase), secretada pela Stenotrophomonas maltophilia, desenvolveu papel decisivo na potencialização da infecção pelo vírus influenza. Essa atividade proteolítica foi detectada pelo teste de ágar-caseína. Amostras positivas para o vírus influenza isolado em animais, bem como em humanos tiveram potencialização da infectividade (ECP) em células MDCK e NCI-H292, sempre que a Stenotrophomonas maltophilia esteve presente. Os referidos microorganismos, bactéria e vírus foram observados ultra-estruturalmente. Esses achados in vitro demonstram como complicações respiratórias podem ocorrer in vivo, através da contribuição de protease microbiana, provocando aumento da inflamação ou destruição dos inibidores celulares de proteases endógenas, nos hospedeiros susceptíveis à influenza.
Descritores: Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Orthomyxoviridae/microbiologia
Orthomyxoviridae/isolamento & purificação
Stenotrophomonas maltophilia/isolamento & purificação
-Ativação Enzimática
Infecções por Bactérias Gram-Negativas/complicações
Cavalos
Influenza Humana/complicações
Influenza Humana/microbiologia
Microscopia Eletrônica
Infecções por Orthomyxoviridae/complicações
Orthomyxoviridae/patogenicidade
Orthomyxoviridae/ultraestrutura
Elastase Pancreática/biossíntese
Stenotrophomonas maltophilia/enzimologia
Suínos
Ativação Viral
Limites: Animais
Bovinos
Humanos
Responsável: BR1.1 - BIREME


  5 / 331 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-973486
Autor: Zhang, Liping; Zhang, Ying; Yu, Xiaofeng; Xu, Huali; Sui, Dayuan; Zhao, Xuezhong.
Título: Alprostadil attenuates myocardial ischemia/reperfusion injury by promoting antioxidant activity and eNOS activation in rats
Fonte: Acta cir. bras;33(12):1067-1077, Dec. 2018. graf.
Idioma: en.
Projeto: Industrial Technology Research Project of Jilin Province.
Resumo: Abstract Purpose: To investigate the effect of alprostadil on myocardial ischemia/reperfusion (I/R) in rats. Methods: Rats were subjected to myocardial ischemia for 30 min followed by 24h reperfusion. Alprostadil (4 or 8 μg/kg) was intravenously administered at the time of reperfusion and myocardial infarct size, levels of troponin T, and the activity of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) in the serum were measured. Antioxidative parameters, nitric oxide (NO) content and phosphorylated endothelial nitric oxide synthase 3 (p-eNOS) expression in the left ventricles were also measured. Histopathological examinations of the left ventricles were also performed. Results: Alprostadil treatment significantly reduced myocardial infarct size, serum troponin T levels, and CK-MB and LDH activity (P<0.05). Furthermore, treatment with alprostadil significantly decreased malondialdehyde (MDA) content (P<0.05) and markedly reduced myonecrosis, edema and infiltration of inflammatory cells. Superoxide dismutase and catalase activities (P<0.05), NO level (P<0.01) and p-eNOS (P<0.05) were significantly increased in rats treated with alprostadil compared with control rats. Conclusion: These results indicate that alprostadil protects against myocardial I/R injury and that these protective effects are achieved, at least in part, via the promotion of antioxidant activity and activation of eNOS.
Descritores: Alprostadil/farmacologia
Traumatismo por Reperfusão Miocárdica/prevenção & controle
Óxido Nítrico Sintase Tipo III/metabolismo
Antioxidantes/farmacologia
-Superóxido Dismutase/análise
Traumatismo por Reperfusão Miocárdica/metabolismo
Traumatismo por Reperfusão Miocárdica/patologia
Catalase/análise
Distribuição Aleatória
Western Blotting
Reprodutibilidade dos Testes
Resultado do Tratamento
Ratos Sprague-Dawley
Estresse Oxidativo/efeitos dos fármacos
Troponina T/efeitos dos fármacos
Troponina T/sangue
Ativação Enzimática/efeitos dos fármacos
Creatina Quinase Forma MB/efeitos dos fármacos
Creatina Quinase Forma MB/sangue
Ventrículos do Coração/efeitos dos fármacos
Ventrículos do Coração/patologia
L-Lactato Desidrogenase/efeitos dos fármacos
L-Lactato Desidrogenase/sangue
Malondialdeído/análise
Infarto do Miocárdio/patologia
Óxido Nítrico/análise
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  6 / 331 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-828186
Autor: Ellepola, Arjuna N. B; Samaranayake, L. P; Khan, Z. U.
Título: Extracellular phospholipase production of oral Candida albicans isolates from smokers, diabetics, asthmatics, denture wearers and healthy individuals following brief exposure to polyene, echinocandin and azole antimycotics
Fonte: Braz. j. microbiol;47(4):911-916, Oct.-Dec. 2016. tab.
Idioma: en.
Projeto: Kuwait University Research Grant.
Resumo: Abstract Objective Candida albicans is the primary causative agent of oral candidosis, and one of its key virulent attributes is considered to be its ability to produce extracellular phospholipases that facilitate cellular invasion. Oral candidosis can be treated with polyenes, and azoles, and the more recently introduced echinocandins. However, once administered, the intraoral concentration of these drugs tend to be sub-therapeutic and rather transient due to factors such as the diluent effect of saliva and cleansing effect of the oral musculature. Hence, intra-orally, the pathogenic yeasts may undergo a brief exposure to antifungal drugs. We, therefore, evaluated the phospholipase production of oral C. albicans isolates following brief exposure to sub-therapeutic concentrations of the foregoing antifungals. Materials and methods Fifty C. albicans oral isolates obtained from smokers, diabetics, asthmatics using steroid inhalers, partial denture wearers and healthy individuals were exposed to sub-therapeutic concentrations of nystatin, amphotericin B, caspofungin, ketoconazole and fluconazole for one hour. Thereafter the drugs were removed and the phospholipase production was determined by a plate assay using an egg yolk-agar medium. Results The phospholipase production of these isolates was significantly suppressed with a percentage reduction of 10.65, 12.14, 11.45 and 6.40% following exposure to nystatin, amphotericin B, caspofungin and ketoconazole, respectively. This suppression was not significant following exposure to fluconazole. Conclusions Despite the sub-therapeutic, intra oral, bioavailability of polyenes, echinocandins and ketoconazole, they are likely to produce a persistent antifungal effect by suppressing phospholipase production, which is a key virulent attribute of this common pathogenic yeast.
Descritores: Fosfolipases/biossíntese
Candida albicans/efeitos dos fármacos
Candida albicans/metabolismo
Candidíase Bucal/microbiologia
Candidíase Bucal/tratamento farmacológico
Antifúngicos/farmacologia
-Polienos/uso terapêutico
Polienos/farmacologia
Azóis/uso terapêutico
Azóis/farmacologia
Candida albicans/isolamento & purificação
Candida albicans/patogenicidade
Fumar
Testes de Sensibilidade Microbiana
Dentaduras
Fatores de Virulência
Diabetes Mellitus
Ativação Enzimática
Espaço Extracelular
Equinocandinas/farmacologia
Antifúngicos/uso terapêutico
Limites: Humanos
Responsável: BR1.1 - BIREME


  7 / 331 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-788949
Autor: Peralta, Guillermo Hugo; Bergamini, Carina Viviana; Hynes, Erica Rut.
Título: Aminotransferase and glutamate dehydrogenase activities in lactobacilli and streptococci
Fonte: Braz. j. microbiol;47(3):741-748, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Aminotransferases and glutamate dehydrogenase are two main types of enzymes involved in the initial steps of amino acid catabolism, which plays a key role in the cheese flavor development. In the present work, glutamate dehydrogenase and aminotransferase activities were screened in twenty one strains of lactic acid bacteria of dairy interest, either cheese-isolated or commercial starters, including fifteen mesophilic lactobacilli, four thermophilic lactobacilli, and two streptococci. The strains of Streptococcus thermophilus showed the highest glutamate dehydrogenase activity, which was significantly elevated compared with the lactobacilli. Aspartate aminotransferase prevailed in most strains tested, while the levels and specificity of other aminotransferases were highly strain- and species-dependent. The knowledge of enzymatic profiles of these starter and cheese-isolated cultures is helpful in proposing appropriate combinations of strains for improved or increased cheese flavor.
Descritores: Streptococcus/enzimologia
Glutamato Desidrogenase/metabolismo
Transaminases/metabolismo
Lactobacillus/enzimologia
-Sistema Livre de Células
Ativação Enzimática
Microbiologia de Alimentos
Limites: Humanos
Responsável: BR1.1 - BIREME


  8 / 331 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-828205
Autor: Aliabadi, Nasrin; Aminzadeh, Saeed; Karkhane, Ali Asghar; Haghbeen, Kamahldin.
Título: Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
Fonte: Braz. j. microbiol;47(4):931-940, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: International Foundation for Science; . Committee on Scientific and Technological Cooperation; . National Institute of Genetic Engineering and Biotechnology.
Resumo: Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and temperature of incubation were the most significant factors influencing chitinase production. From the tested values, the best pH/temperature was obtained at pH 5 and 70 °C, with Km and V max values of chitinase to be 5.6 mg/mL and 0.87 µmol/min, respectively. Ag+, Co2+, iodoacetamide, and iodoacetic acid inhibited the enzyme activity, whereas Mn2+, Cu2+, Tweens (20 and 80), Triton X-100, and EDTA increased the same. In addition, the study of the morphological alteration of chitin treated by enzyme by SEM revealed cracks and pores on the chitin surface, indicating a potential application of this enzyme in several industries.
Descritores: Bacillus/metabolismo
Quitinases/metabolismo
-Fósforo/metabolismo
Temperatura
Bacillus/isolamento & purificação
Bacillus/genética
Bacillus/ultraestrutura
Estabilidade Enzimática/efeitos dos fármacos
Carbono/metabolismo
RNA Ribossômico 16S/genética
Cinética
Quitinases/química
Análise de Sequência de DNA
Ativação Enzimática
Concentração de Íons de Hidrogênio
Íons
Metais
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  9 / 331 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-788974
Autor: Gururaj, P; Ramalingam, Subramanian; Devi, Ganesan Nandhini; Gautam, Pennathur.
Título: Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
Fonte: Braz. j. microbiol;47(3):647-657, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: Department of Biotechnology, Government of India.
Resumo: ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a 0.5% (v/v) inoculum, 2% (v/v) castor oil (inducer), and agitation 150 rpm. The optimized conditions from the shake flask experiments were validated in a 3 L lab scale bioreactor, and the lipase production increased to 48 U/mL. The enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography and the overall yield was 36%. SDS-PAGE indicated a molecular weight of 45 kDa for the purified protein, and Matrix assisted laser desorption/ionization time of flight analysis of the purified lipase showed sequence similarity with GDSL family of lipases. The optimum temperature and pH for activity of the enzyme was found to be 50 ºC and 8.0, respectively. The lipase was completely inhibited by phenylmethylsulfonyl fluoride but minimal inhibition was observed when incubated with ethylenediaminetetraacetic acid and dithiothreitol. The enzyme was stable in the presence of non-polar hydrophobic solvents. Detergents like SDS inhibited enzyme activity; however, there was minimal loss of enzyme activity when incubated with hydrogen peroxide, Tween 80 and Triton X-100. The kinetic constants (Km and Vmax) revealed that the hydrolytic activity of the lipase was specific to moderate chain fatty acid esters. The Vmax, Km and Vmax/Km ratio of the enzyme were 16.98 U/mg, 0.51 mM, and 33.29, respectively when 4-nitrophenyl palmitate was used as a substrate.
Descritores: Compostos Orgânicos
Solventes
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/biossíntese
Acinetobacter/enzimologia
Lipase/isolamento & purificação
Lipase/biossíntese
-Compostos Orgânicos/química
Solventes/química
Especificidade por Substrato
Temperatura
Proteínas de Bactérias/química
Estabilidade Enzimática
Cinética
Cromatografia por Troca Iônica
Ativação Enzimática
Espaço Extracelular/enzimologia
Concentração de Íons de Hidrogênio
Íons
Lipase/química
Lipólise
Metais
Peso Molecular
Responsável: BR1.1 - BIREME


  10 / 331 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-788971
Autor: Ouf, Salama A; Moussa, Tarek A; Abd-Elmegeed, Alshimaa M; Eltahlawy, Samar R.
Título: Anti-fungal potential of ozone against some dermatophytes
Fonte: Braz. j. microbiol;47(3):697-702, July-Sept. 2016. tab.
Idioma: en.
Resumo: ABSTRACT Dermatophytes are classified in three genera, Epidermophyton, Microsporum and Trichophyton. They have the capacity to invade keratinized tissue to produce a cutaneous infection known as dermatophytoses. This investigation was performed to study the effect of gaseous ozone and ozonized oil on three specific properties of six different dermatophytes. These properties included sporulation, mycelia leakage of sugar and nutrients and the activity of their hydrolytic enzymes. Generally, ozonized oil was found to be more efficacious than gaseous ozone. Microsporum gypseum and Microsporum canis were the most susceptible, while Trichophyton interdigitale and T. mentagrophytes were relatively resistant. The study revealed a steady decline in spore production of M. gypseum and M. canis on application of ozonated oil. An increase in leakage of electrolytes and sugar was noticed after treatment with ozonized oil in the case of M. gypseum, M. canis, T. interdigitale, T. mentagrophytes and T. rubrum. The results also revealed loss in urease, amylase, alkaline phosphatase, lipase and keratinase enzyme producing capacity of the investigated fungi.
Descritores: Ozônio/farmacologia
Arthrodermataceae/efeitos dos fármacos
Antifúngicos/farmacologia
-Permeabilidade
Esporos Fúngicos/efeitos dos fármacos
Proteínas Fúngicas/metabolismo
Micélio
Arthrodermataceae/fisiologia
Eletrólitos/metabolismo
Ativação Enzimática
Metabolismo dos Carboidratos/efeitos dos fármacos
Limites: Humanos
Responsável: BR1.1 - BIREME



página 1 de 34 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde