Base de dados : LILACS
Pesquisa : G02.111.385 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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Texto completo SciELO Brasil
Amaral, Ana Claudia F.
Texto completo
Id: lil-748265
Autor: Ramos, Aline de Souza; Ribeiro, Joyce Benzaquem; Teixeira, Bruna Gomes; Ferreira, José Luiz Pinto; Silva, Jefferson Rocha de A.; Ferreira, Alexandre do Amaral; Souza, Rodrigo Octavio Mendonça Alves de; Amaral, Ana Claudia F..
Título: Hydroxylation of 1,8-cineole by Mucor ramannianus and Aspergillus niger
Fonte: Braz. j. microbiol;46(1):261-264, 05/2015. tab, graf.
Idioma: en.
Resumo: The monoterpenoid 1,8-cineole is obtained from the leaves of Eucalyptus globulus and it has important biological activities. It is a cheap natural substrate because it is a by-product of the Eucalyptus cultivation for wood and pulp production. In this study, it was evaluated the potential of three filamentous fungi in the biotransformation of 1,8-cineole. The study was divided in two steps: first, reactions were carried out with 1,8-cineole at 1 g/L for 24 h; afterwards, reactions were carried out with substrate at 5 g/L for 5 days. The substrate was hydroxylated into 2-exo-hydroxy-1,8-cineole and 3-exo-hydroxy-1,8-cineole by fungi Mucor ramannianus and Aspergillus niger with high stereoselectivity. Trichoderma harzianum was also tested but no transformation was detected. M. ramannianus led to higher than 99% of conversion within 24 h with a starting high substrate concentration (1 g/L). When substrate was added at 5 g/L, only M. ramannianus was able to catalyze the reaction, but the conversion level was 21.7% after 5 days. Both products have defined stereochemistry and could be used as chiral synthons. Furthermore, biological activity has been described for 3-exo-hydroxy-1,8-cineol. To the best of our knowledge, this is the first report on the use of M. ramannianus in this reaction.
Descritores: Aspergillus niger/metabolismo
Fatores de Tempo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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Texto completo SciELO Chile
Texto completo
Id: lil-490635
Autor: Varela, Nelson M; Quiñones, Luis A; Orellana, Myriam; Poniachik, Jaime; Csendes, Attila; Smok, Gladys; Rodrigo, Ramón; Cáceres, Dante D; Videla, Luis A.
Título: Study of Cytochrome P450 2E1 and its allele Variants in Liver Injury of Nondiabetic, Nonalcoholic Steatohepatitis Obese Women
Fonte: Biol. Res;41(1):81-92, 2008. ilus, tab, graf.
Idioma: en.
Projeto: FONDECYT.
Resumo: CYP2E1 enzyme is related to nonalcoholic steatohepatitis (NASH) due to its ability for reactive oxygen species production, which can be influenced by polymorphisms in the gene. The aim of this study was to investigate hepatic levels, activity, and polymorphisms of the CYP2E1 gene to correlate it with clinical and histological features in 48 female obese NASH patients. Subjects were divided into three groups: (i) normal; (ii) steatosis; and (iii) steatohepatitis. CYP2E1 protein level was assayed in microsomes from liver biopsies, and in vivo chlorzoxazone hydroxylation was determined by HPLC. Genomic DNA was isolated for genotype analysis through PCR. The results showed that liver CYP2E1 content was significantly higher in the steatohepatitis (45 percent; p=0.024) and steatosis (22 percent; p=0.032) group compared with normal group. Chlorzoxazone hydroxylase activity showed significant enhancement in the steatohepatitis group (15 percent, p=0.027) compared with the normal group. c2 rare allele of RsallPstl polymorphisms but no C allele of Dral polymorphism was positively associated with CHZ hydroxylation, which in turn is correlated with liver CYP2E1 content (r=0.59; p=0.026). In conclusion, c2 allele is positively associated with liver injury in NASH. This allele may determine a higher transcriptional activity of the gene, with consequent enhancement in pro-oxidant activity of CYP2E1 thus affording liver toxicity.
Descritores: /metabolismo
Fígado Gorduroso/enzimologia
-Estudos de Casos e Controles
Cromatografia Líquida de Alta Pressão
Fígado Gorduroso/patologia
Frequência do Gene
Polimorfismo Genético
Limites: Adulto
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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Id: lil-356329
Autor: Linares, Edlaine.
Título: Papel do peroxinitrito na atividade leishmanicida de macrófagos em modelos murinos / Paper of the peroxynitrite in the leishmanicidal activity of macrophages in murine models.
Fonte: São Paulo; s.n; 2003. 79 p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Instituto de Química para obtenção do grau de Doutor.
Resumo: Os mecanismos oxidativos pelos quais macrófagos exercem atividade microbicida permanecem em discussão, e estudos com hospedeiros animais serão essenciais para elucidar tal questão. Neste trabalho, estudamos os mecanismos microbicidas de macrófagos in vivo comparando parâmetros de infecção nas lesões de camundongos resistentes (C57B1/6) e suscetíveis (BALB/c) ao protozoário Leishmania amazonensis. A comparação mostrou que o controle da infecção pelos camundongos resistentes é dependente da ativação de macrófagos com expressão da enzima óxido nítrico sintase induzível, síntese de óxido nítrico e extensa nitração e hidroxilação das proteínas dos parasitas dentro dos fagolisossomos dos macrófagos...
Descritores: Bioquímica
Radicais Livres
Ativação de Macrófagos
Óxido Nítrico
-Espectroscopia de Ressonância de Spin Eletrônica
Controle de Infecções
Limites: Animais
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas
BR40.1; 574.192

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Texto completo SciELO Brasil
Texto completo
Id: lil-260244
Autor: Pereira, V. A; Auler Junior, J. O; Carmona, M. J; Mateus, F. H; Lanchote, V. L; Breimer, D. D; Santos, S. R. C. J.
Título: A micromethod for quantitation of debrisoquine and 4-hydroxydebrisoquine in urine by liquid chromatography
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;33(5):509-14, May 2000. graf.
Idioma: en.
Projeto: FAPESP.
Resumo: We describe a new simple, selective and sensitive micromethod based on HPLC and fluorescence detection to measure debrisoquine (D) and 4-hydroxydebrisoquine (4-OHD) in urine for the investigation of xenobiotic metabolism by debrisoquine hydroxylase (CYP2D6). Four hundred µl of urine was required for the analysis of D and 4-OHD. Peaks were eluted at 8.3 min (4-OHD), 14.0 min (D) and 16.6 min for the internal standard, metoprolol (20 µg/ml). The 5-µm CN-reverse-phase column (Shimpack, 250 x 4.6 mm) was eluted with a mobile phase consisting of 0.25 M acetate buffer, pH 5.0, and acetonitrile (9:1, v/v) at 0.7 ml/min with detection at lexcitation = 210 nm and lemission = 290 nm. The method, validated on the basis of measurements of spiked urine, presented 3 ng/ml (D) and 6 ng/ml (4-OHD) sensitivity, 390-6240 ng/ml (D) and 750-12000 ng/ml (4-OHD) linearity, and 5.7/8.2 percent (D) and 5.3/8.2 percent (4-OHD) intra/interassay precision. The method was validated using urine of a healthy Caucasian volunteer who received one 10-mg tablet of Declinax®, po, in the morning after an overnight fast. Urine samples (diuresis of 4 or 6 h) were collected from zero to 24 h. The urinary excretion of D and 4-OHD, Fel (0-24 h), i.e., fraction of dose administered and excreted into urine, was 6.4 percent and 31.9 percent, respectively. The hydroxylation capacity index reported as metabolic ratio was 0.18 (D/4-OHD) for the person investigated and can be compared to reference limits of < 12.5 for poor metabolizers (PM) and < 12.5 for extensive metabolizers (EM). In parallel, the recovery ratio (RR), another hydroxylation capacity index, was 0.85 (4-OHD: SD + 4-OHD) versus reference limits of RR < 0.12 for PM and RR > 0.12 for EM. The healthy volunteer was considered to be an extensive metabolizer on the basis of the debrisoquine test.
Descritores: Cromatografia Líquida de Alta Pressão/métodos
Citocromo P-450 CYP2D6/metabolismo
-Intervalos de Confiança
Grupo com Ancestrais do Continente Europeu
Sensibilidade e Especificidade
Limites: Humanos
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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