Base de dados : LILACS
Pesquisa : G02.111.528 [Categoria DeCS]
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Id: biblio-1087705
Autor: Sagia, Sajish; Sharma, Anamika; Singh, Surender; Chaturvedi, Shivani; Singh Nain, Pawan Kumar; Nain, Lata.
Título: Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw
Fonte: Electron. j. biotechnol;44:60-68, Mar. 2020. tab, graf, ilus.
Idioma: en.
Resumo: Background: Oleaginous yeasts can be grown on different carbon sources, including lignocellulosic hydrolysate containing a mixture of glucose and xylose. However, not all yeast strains can utilize both the sugars for lipogenesis. Therefore, in this study, efforts were made to isolate dual sugar-utilizing oleaginous yeasts from different sources. Results: A total of eleven isolates were obtained, which were screened for their ability to utilize various carbohydrates for lipogenesis. One promising yeast isolate Trichosporon mycotoxinivorans S2 was selected based on its capability to use a mixture of glucose and xylose and produce 44.86 ± 4.03% lipids, as well as its tolerance to fermentation inhibitors. In order to identify an inexpensive source of sugars, nondetoxified paddy straw hydrolysate (saccharified with cellulase), supplemented with 0.05% yeast extract, 0.18% peptone, and 0.04% MgSO4 was used for growth of the yeast, resulting in a yield of 5.17 g L−1 lipids with conversion productivity of 0.06 g L−1 h−1 . Optimization of the levels of yeast extract, peptone, and MgSO4 for maximizing lipid production using Box­Behnken design led to an increase in lipid yield by 41.59%. FAME analysis of single cell oil revealed oleic acid (30.84%), palmitic acid (18.28%), and stearic acid (17.64%) as the major fatty acids. Conclusion: The fatty acid profile illustrates the potential of T. mycotoxinivorans S2 to produce single cell oil as a feedstock for biodiesel. Therefore, the present study also indicated the potential of selected yeast to develop a zero-waste process for the complete valorization of paddy straw hydrolysate without detoxification
Descritores: Trichosporon/metabolismo
-Oryza
Xilose/isolamento & purificação
Trichosporon/química
Óleos/química
Lipogênese
Biocombustíveis
Fermentação
Glucose/isolamento & purificação
Hidrólise
Lignina/metabolismo
Lipídeos/biossíntese
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-914719
Autor: Villarroel, Pía; Cifuentes, Mariana.
Título: Efecto de la activación del receptor sensor de calcio sobre la expresión de genes lipogénicos en células HepG2 / Effect of calcium sensing receptor activation on lipogenic gene expression in HepG2 cells
Fonte: Rev. chil. endocrinol. diabetes;11(2):47-53, abr. 2018. tab, graf.
Idioma: es.
Projeto: FONDECYT.
Resumo: Introduction: The Calcium Sensing Receptor (CaSR) is expressed in human fat cells, and its stimulation may be associated with adipose tissue dysfunction. The multisystemic character of obesity and the search of deepening the scope of the activation of CaSR in this disorder allows us to study the response of this protein in tissues that differ from adipose. Objective: To evaluate the effect of CaSR activation on the expression of lipogenic genes in a model of excess glucose and fatty acids in HepG2 human liver cells. Materials and methods: The effect of the calcimimetic cinacalcet (allosteric agonist of CaSR) on the content of triglycerides (fluorimetry) in a model of glucose supply and on the expression of lipogenic genes (qPCR) in hyperglycemia and hyperlipidemia conditions in the Liver cell line HepG2. Results: Cinacalcet, glucose (25 mM) and oleic acid (0.6 mM) did not affect cell viability. Activation of CaSR in the presence of glucose failed to increase the intracellular triglyceride content at 72 hours. Under these conditions, no response was observed for the factors coding for lipogenic genes (SREBP1c and FAS) at 24 hours of stimulation with cinacalcet in the liver cells. In the case of the over supply of fatty acids, the HepG2 cells did not show a variation in the gene expression of the DGAT enzymes after exposure to cinacalcet. Conclusion:Under conditions of glucose exposure, cinacalcet did not show a response in the triglyceride content, nor in the expression of genes related to hepatic lipogenesis. Therefore, stimulation of CaSR would not be associated with hepatic steatosis in HepG2 cells exposed to glucose.
Descritores: Receptores de Detecção de Cálcio
Lipogênese
Células Hep G2
-Sobrevivência Celular
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-935999
Autor: Cruz, André Luiz de Souza.
Título: Papel dos corpúsculos lipídicos na progressão do ciclo celular.
Fonte: Rio de Janeiro; s.n; 2010. 152 p. ilus, graf.
Idioma: pt.
Tese: Apresentada a Instituto Nacional de Câncer para obtenção do grau de Mestre.
Resumo: Corpúsculos lipídicos são organelas dinâmicas envolvidas no metabolismo de lipídeos, no tráfego de membrana e na sinalização intracelular. A lipogênese está associada com um mal prognóstico em diversas doenças neoplásicas, sugerindo um papel dessas organelas no desenvolvimento do câncer. Foi mostrado anteriormente que corpúsculos lipídicos são elementos centrais na síntese de PGE2 e na proliferação celular em linhagens celulares de câncer de cólon, e podem estar implicados na patologia do adenocarcinoma de cólon. Baseado nesses dados, a regulação dos corpúsculos lipídicos na progressão do ciclo celular foi investigada. Células NIH3T3 foram sincronizadas por privação de soro por 24 horas, e então suplementadas com meio contendo 10% de soro fetal bovino para avaliar sua entrada e progressão pelo ciclo celular. Análise por iodeto de propídeo revelou que, a partir da suplementação com soro, células entraram na fase S do ciclo celular após 24 horas e progrediram para as fases G2/M após 36 e 48 horas. Esses dados foram confirmados com análise dos níveis de fosforilação de Rb por western blot e dos níveis de expressão das ciclinas D2, E2, A2 e B2 por PCR em tempo real. Posteriormente, a regulação dos corpúsculos lipídicos durante a progressão do ciclo celular foi avaliada. Células com arresto na fase G1 mostraram um menor número de corpúsculos lipídicos, com localização perinuclear. Por outro lado, foram observados um aumento no número de corpúsculos lipídicos e uma distribuição homogênea dessas organelas pelo citoplasma durante a fase S. Além disso, células NIH3T3 mostraram aumento no número de corpúsculos lipídicos e localização dispersa dessas organelas após transformação com a oncoproteína H-rasV12. Juntos esses resultados mostram que corpúsculos lipídicos são regulados durante a progressão do ciclo celular, que esta regulação depende da entrada na fase S do ciclo celular, e que em células transformadas esta regulação está alterada. Por fim, esses dados dão evidências de que um mecanismo coordenado regula a progressão de ciclo celular e a biogênese de corpúsculos lipídicos, que pode estar desregulado durante o desenvolvimento neoplásico

Lipid bodies are dynamic organelles involved in lipid turnover, membrane traffic and intracellular signaling. Lipogenesis has been associated with poor prognosis in several neoplasic diseases, suggesting a role for these organelles in cancer development. It has been previously reported that lipid bodies are centrally involved in PGE2 synthesis and cell proliferation in colon cancer cells, and may potentially have implications to colon adenocarcinoma pathogenesis. Based on this data, the regulation of lipid bodies during cell cycle progression was investigated. NIH3T3 cells were synchronized by serum starvation for 24 hours, and then supplemented with medium containing 10% fetal bovine serum to evaluate their entering and progression through cell cycle. Propidium iodide analysis revealed that upon serum supplementation cells reached S phase after 24 hours and followed to G2/M phase after 36-48 hours. These data was confirmed by analysis of Rb phosphorylation by western blot assay and by expression levels of cyclins D2, E2, A2, and B2 assayed by quantitative real-time PCR. The quantity and subcellular localization of lipid bodies during cell cycle progression was evaluated. Cells arrested on G1 phase showed a lower number of lipid bodies with perinuclear localization. On the other hand, an increased number of lipid bodies with a homogeneous distribution through the cytoplasm were observed during S phase. Moreover, NIH3T3 cells displayed increased number and dispersed localization of lipid bodies upon transformation with H-rasV12 oncoprotein. Taken together, these results suggest that lipid bodies are highly regulated during cell cycle, and also that this regulation is altered in transformed cells. Finally, these data provide evidence for a coordinate mechanism that regulates cell cycle progression and lipid body biogenesis, which might be deregulated during cancer development
Descritores: Ciclo Celular
Transformação Celular Neoplásica
Lipogênese
Responsável: BR440.4 - Biblioteca
BR440.1


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Id: biblio-849064
Autor: Almeida, Felipe Natali; Andrade, Maynara Lucca; Moreira, Gabriela Virginia; Camporez, João Paulo Gabriel; Chimin, Patricia; Carvalho, Carla Roberta de Oliveira.
Título: DHEA and non-alcoholic fat liver disease: increased gene expression of peroxisome proliferation-activated receptor γ (PPARγ) and fatty acid synthase (FAS) / DHEA e doença gordurosa hepática não-alcoólica: aumento na expressão gênica do peroxisome proliferation-activated receptor γ (PPARγ) e ácido graxo sintase (FAS)
Fonte: Acta sci., Biol. sci;36(2):223-229, abr.- jun. 2014. tab, ilus.
Idioma: en.
Resumo: Dehydroespiandrosterone (DHEA) is associated with improvements in chronic degenerative diseases, including obesity, insulin resistance, and cardiovascular diseases. Nevertheless, it is observed an increase in its concentration in individuals with liver lipid infiltration, but it is not precise if this condition emerges as a cause or a consequence. In this way, we aimed to identify gene expression alterations in lipid and glucose liver metabolism markers, as well as oxidative stress markers. For this purpose, male Wistar rats, 12-14 months old were treated with subcutaneous injections of DHEA (only dose of 10 mg kg-1); and after 7 days, hepatic gene expression by PCR real time were performed for the following genes: G6Pase, PEPCK, FAS, PPARγ, malic enzyme, ChREBP, LXR, catalase, GPx, iNOS, NADPH oxidase subunits and PCNA. We observed a tendency of reduction in G6Pase gene expression in treated group (p = 0.08). In addition, it was identified an increase in liver PPARγ and FAS gene expressions, two markers of increased activity of lipogenic pathway. We also observed an increase in iNOS gene expression, a known inductor of systemic and hepatic insulin resistance. In conclusion, our data indicates that the treatment with DHEA can be associated with the development of liver lipid infiltration and hepatic insulin resistance.

A deidroepiandrosterona (DHEA) encontra-se associada a melhorias em quadros de obesidade, resistência à insulina e doenças cardiovasculares. Porém, observa-se um aumento na sua concentração em indivíduos portadores de infiltração lipídica hepática, mas sem saber precisar se o mesmo surge como causa ou consequência. Assim, objetivamos identificar alterações na expressão gênica hepática de marcadores relacionados ao metabolismo lipídico e glicídico e de estresse oxidativo. Para tanto, ratos machos com 12-14 meses de idade foram tratados com injeção subcutânea de DHEA (dose única 10 mg kg-1), e após 7 dias foram feitas análises da expressão gênica hepática por PCR em tempo real das seguintes proteínas: G6Pase, PEPCK, FAS, PPARγ, enzima málica, ChREBP, LXR, catalase, GPx, iNOS, subunidades da NADPHoxidase e PCNA. Observamos uma tendência à redução da expressão gênica da G6Pase no grupo tratado (p = 0,08). Também identificamos um aumento na expressão gênica hepática do PPARγ e FAS, dois indicadores de aumento da atividade das vias de lipogênese. Observamos um aumento na expressão gênica da iNOS, um conhecido agente indutor de resistência insulina sistêmica e hepática. Em conclusão, nossos dados indicam que o tratamento com DHEA pode estar associado com o desenvolvimento de um quadro de infiltração lipídica hepática e resistência à insulina hepática.
Descritores: Desidroepiandrosterona
Fígado Gorduroso
Lipogênese
Responsável: BR513.1 - Biblioteca Central


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Id: biblio-839295
Autor: Cantelli, KR; Soares, GM; Ribeiro, RA; Balbo, SL; Lubaczeuski, C; Boschero, AC; Araújo, ACF; Bonfleur, ML.
Título: Duodenal-jejunal bypass normalizes pancreatic islet proliferation rate and function but not hepatic steatosis in hypothalamic obese rats
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(5):e5858, 2017. tab, graf.
Idioma: en.
Projeto: Fundação Araucária; . FAPESP.
Resumo: Modifications in life-style and/or pharmacotherapies contribute to weight loss and ameliorate the metabolic profile of diet-induced obese humans and rodents. Since these strategies fail to treat hypothalamic obesity, we have assessed the possible mechanisms by which duodenal-jejunal bypass (DJB) surgery regulates hepatic lipid metabolism and the morphophysiology of pancreatic islets, in hypothalamic obese (HyO) rats. During the first 5 days of life, male Wistar rats received subcutaneous injections of monosodium glutamate (4 g/kg body weight, HyO group), or saline (CTL). At 90 days of age, HyO rats were randomly subjected to DJB (HyO DJB group) or sham surgery (HyO Sham group). HyO Sham rats were morbidly obese, insulin resistant, hypertriglyceridemic and displayed higher serum concentrations of non-esterified fatty acids (NEFA) and hepatic triglyceride (TG). These effects were associated with higher expressions of the lipogenic genes and fatty acid synthase (FASN) protein content in the liver. Furthermore, hepatic genes involved in β-oxidation and TG export were down-regulated in HyO rats. In addition, these rats exhibited hyperinsulinemia, β-cell hypersecretion, a higher percentage of islets and β-cell area/pancreas section, and enhanced nuclear content of Ki67 protein in islet-cells. At 2 months after DJB surgery, serum concentrations of TG and NEFA, but not hepatic TG accumulation and gene and protein expressions, were normalized in HyO rats. Insulin release and Ki67 positive cells were also normalized in HyO DJB islets. In conclusion, DJB decreased islet-cell proliferation, normalized insulinemia, and ameliorated insulin sensitivity and plasma lipid profile, independently of changes in hepatic metabolism.
Descritores: Duodeno/cirurgia
Fígado Gorduroso/metabolismo
Derivação Gástrica/métodos
Doenças Hipotalâmicas/metabolismo
Ilhotas Pancreáticas/citologia
Ilhotas Pancreáticas/metabolismo
Jejuno/cirurgia
Obesidade/metabolismo
-Animais Recém-Nascidos
Glicemia/metabolismo
Proliferação de Células
Colesterol/sangue
Ácido Graxo Sintase Tipo I/metabolismo
Ácidos Graxos/sangue
Fígado Gorduroso/fisiopatologia
Doenças Hipotalâmicas/fisiopatologia
Doenças Hipotalâmicas/cirurgia
Resistência à Insulina
Insulina/metabolismo
Ilhotas Pancreáticas/fisiopatologia
Lipogênese/genética
Fígado/metabolismo
Fígado/patologia
Obesidade/fisiopatologia
Obesidade/cirurgia
Pâncreas/metabolismo
Pâncreas/patologia
Distribuição Aleatória
Ratos Wistar
Reprodutibilidade dos Testes
Fatores de Tempo
Triglicerídeos/sangue
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-772346
Autor: Lannes, Wilian Rodrigues; Miranda, Andressa Cabral de; Souza-Mello, Vanessa de; Barbosa-da-Silva, Sandra; Aguila, Marcia Barbosa; Mandarim-de-Lacerda, Carlos Alberto.
Título: Both hepatic lipogenesis and beta-oxidation are altered in offspring of mothers fed a high-fat diet in the first two generations (F1 and F2) / La lipogénesis hepática y la beta-oxidación están alteradas en las crías de madres alimentadas con una dieta alta en grasas en las dos primeras generaciones (F1 y F2)
Fonte: Int. j. morphol;33(4):1510-1517, Dec. 2015. ilus.
Idioma: en.
Resumo: The high fat (HF) fed mothers may program susceptibility in offspring to chronic diseases and affect subsequent generations. The present study evaluated the liver structure in adulthood, focusing on the F1 and F2 generations. Females C57BL/6 (F0) were fed standard chow (SC) or HF diet (8 weeks) prior to mating and during the gestation and lactation to provide the F1 generation (SC-F1 and HF-F1). All other mothers and offspring fed SC. At 3 months old, F1 females were mated to produce the F2 generation (SC-F2 and HF-F2). The liver was kept in several fragments and prepared for histological analysis or frozen for biochemical and molecular analyzes. The F1 and F2 offspring were studied at 3 months old. HF-F1 had higher body mass (BM) compared to SC-F1 (P= 0.001), but not HF-F2 compared to SC-F2. HF-F1 had glucose intolerance when compared to SC-F1, but not HF-F2 compared to SC-F2. HF-F1 (P= 0.009) and HF-F2 (P= 0.03) showed hyperinsulinemia compared to their counterparts. Both groups HF-F1 and HF-F2 showed more steatosis than the SC counterparts (F1 and F2, P<0.0001). HF-F1 showed increased expression of PPAR-gamma and SREBP1-c compared to SC-F1 (P= 0.01). HF-F2 showed increased PPAR-gamma expression compared to SC-F2 (P= 0.04). In conclusion, HF-fed mother impairs both lipogenesis and beta-oxidation pathways in F1 through upregulation of PPAR-gamma and downregulation of PPAR-alpha. In F2, the only lipogenesis is enhanced, but it causes a disrupted PPAR balance, favoring the hepatic lipid accumulation and impaired metabolism in these animals that were not directly exposed to the maternal HF intake.

Los madres alimentadas con dieta rica en grasas (HF) pueden programar una susceptibilidad al desarrollo de enfermedades crónicas en su descendencia y de este modo afectar a las generaciones posteriores. El presente estudio evaluó la estructura del hígado en la edad adulta, centrándose en las generaciones F1 y F2. Las hembras C57BL/6 (F0) fueron alimentadas con dieta estándar (CS) o dieta HF (8 semanas) antes del apareamiento y durante la gestación y lactancia para producir la generación F1 (CS-F1 y HF-F1). Todas las demás madres y crías fueron alimentadas con CS. A los 3 meses de edad, las hembras F1 fueron apareadas para producir la generación F2 (CS-F2 y HF-F2). El hígado se conservó en varios fragmentos y se preparó, por un lado, para el análisis histológico, y por otro, se lo congeló para realizar análisis bioquímicos y moleculares. La descendencia F1 y F2 se estudió a los 3 meses de edad. HF-F1 tuvo una mayor masa corporal (BM) en comparación con CS-F1 (P= 0,001), pero no el grupo HF-F2 en comparación con CS-F2. HF-F1 tenía intolerancia a la glucosa en comparación con CS-F1, pero no el grupo HF-F2 en comparación con CS-F2. HF-F1 (P= 0,009) y HF-F2 (P= 0,03) mostraron hiperinsulinemia en comparación con sus homólogos. Ambos grupos HF-F1 y HF-F2 mostraron más esteatosis que las contrapartes CS (F1 y F2, P <0,0001). HF-F1 mostró una mayor expresión de PPAR-gamma y SREBP1-c en comparación con el grupo CS-F1 (P= 0,01). HF-F2 mostró aumento de la expresión de PPAR-gamma en comparación con CS-F2 (P= 0,04). En conclusión, la madre alimentada con HF presenta ambas vías afectadas, de lipogénesis y de la beta-oxidación, en la F1 a través de la regulación positiva de PPAR-gamma y con regulación a la baja de los PPAR-alfa. En F2, solo ha mejorado la vía de lipogénesis, pero causa un desbalance de PPAR, lo que favorece la acumulación de lípidos hepáticos y la alteración del metabolismo en estos animales que no estaban directamente expuestos a la ingesta materna de HF.
Descritores: Dieta Hiperlipídica/efeitos adversos
Fígado Gorduroso/patologia
Obesidade/complicações
-Animais Recém-Nascidos
Western Blotting
Hiperinsulinismo
Lipogênese
Camundongos Endogâmicos C57BL
Efeitos Tardios da Exposição Pré-Natal
Limites: Animais
Masculino
Feminino
Gravidez
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-647745
Autor: Higa, T.S.; Bergamo, F.C.; Mazzucatto, F.; Fonseca-Alaniz, M.H.; Evangelista, F.S..
Título: Physical training prevents body weight gain but does not modify adipose tissue gene expression
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;45(10):988-994, Oct. 2012. ilus, tab.
Idioma: en.
Resumo: The relationship of body weight (BW) with white adipose tissue (WAT) mass and WAT gene expression pattern was investigated in mice submitted to physical training (PT). Adult male C57BL/6 mice were submitted to two 1.5-h daily swimming sessions (T, N = 18), 5 days/week for 4 weeks or maintained sedentary (S, N = 15). Citrate synthase activity increased significantly in the T group (P < 0.05). S mice had a substantial weight gain compared to T mice (4.06 ± 0.43 vs 0.38 ± 0.28 g, P < 0.01). WAT mass, adipocyte size, and the weights of gastrocnemius and soleus muscles, lung, kidney, and adrenal gland were not different. Liver and heart were larger and the spleen was smaller in T compared to S mice (P < 0.05). Food intake was higher in T than S mice (4.7 ± 0.2 vs 4.0 ± 0.3 g/animal, P < 0.05) but oxygen consumption at rest did not differ between groups. T animals showed higher serum leptin concentration compared to S animals (6.37 ± 0.5 vs 3.11 ± 0.12 ng/mL). WAT gene expression pattern obtained by transcription factor adipocyte determination and differentiation-dependent factor 1, fatty acid synthase, malic enzyme, hormone-sensitive lipase, adipocyte lipid binding protein, leptin, and adiponectin did not differ significantly between groups. Collectively, our results showed that PT prevents BW gain and maintains WAT mass due to an increase in food intake and unchanged resting metabolic rate. These responses are closely related to unchanged WAT gene expression patterns.
Descritores: Tecido Adiposo Branco/metabolismo
Regulação da Expressão Gênica
Condicionamento Físico Animal/fisiologia
Ganho de Peso/genética
-Adipogenia/genética
Adiponectina/genética
Marcadores Genéticos/genética
Leptina/genética
Lipogênese/genética
Lipólise/genética
MICE, INBRED CABDOMENABDOMINAL INJURIESBL
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-641011
Autor: José, A. A. F. B. V; Gama, M. A. S; Lanna, D. D. P.
Título: Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs
Fonte: Genet. mol. res. (Online);7(2):284-294, 2008. tab.
Idioma: en.
Resumo: The objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 μM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 μM PVA; 3) CLA200: 200 μM trans-10, cis-12 CLA; 4) CLA50: 50 μM trans-10, cis-12 CLA, and 5) LA: 200 μM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on non-esterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis...
Descritores: Ácidos Linoleicos Conjugados/farmacologia
Metabolismo dos Lipídeos
Regulação da Expressão Gênica no Desenvolvimento
Suínos/genética
Tecido Adiposo
-Ácidos Graxos/metabolismo
Ácido Graxo Sintases/genética
Ácido Graxo Sintases/metabolismo
Lipogênese
Lipólise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Técnicas de Cultura de Tecidos
Tecido Adiposo/crescimento & desenvolvimento
Tecido Adiposo/metabolismo
Limites: Animais
Masculino
Responsável: BR26.1 - Biblioteca Central


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Id: lil-553269
Autor: Valenzuela, Carina; Aguirre, Carolina; Castillo, Valeska; Ronco, Ana María; Llanos, Miguel.
Título: Participación del sistema endocanabinoide en el desarrollo de obesidad / A role for the endocannabinoid system in obesity
Fonte: Rev. méd. Chile;138(5):621-629, mayo 2010. ilus.
Idioma: es.
Projeto: Fondecyt.
Resumo: Endocannabinoids are the endogenous ligands for the cannabinoid receptors type 1 and 2. These membrane receptors are responsible for the psychotropic effects of Cannabis Sativa, when bound to its active component known as (-)-Δ9-tetrahydro-cannabinol. Cannabinoid receptors, endocannabinoids and the enzymes catalyzing their biosynthesis and degradation, constitute the endocannabinoid system (ECS), which has a remarkable role controlling energy balance, both at central nervous system and peripheral tissues. The ECS regulates food ingestion by stimulating a network of orexigenic neurons present in the hypothalamus and reinforcing motivation and reward to food consumption in the nucleus accumbens. Regarding peripheral tissues, this system controls lipid and glucose metabolism at different levels, reduces energy expenditure and leads energy balance to fat storage. Metabolic alterations, includ-ing excessive accumulation of abdominal fat, dyslipidaemia and hyperglicaemia, are suggested to be associated to a hyperactivated ECS. Since obesity is one of the major health problems in modern societies, in this review we discuss the role of the endocannabinoid system in metabolic pathways associated to control mechanisms of energy balance and its involvement in overweight and obesity. In addition, we also discuss therapeutic possibilities and emergent problems due to cannabinoid receptor type 1 antagonism utilized as treatment for such alterations.
Descritores: Endocanabinoides/metabolismo
Metabolismo Energético/fisiologia
Lipogênese/fisiologia
Obesidade/metabolismo
Receptor CB1 de Canabinoide/metabolismo
-Lipídeos/biossíntese
Obesidade/tratamento farmacológico
Obesidade/etiologia
Receptor CB1 de Canabinoide/agonistas
Receptor CB1 de Canabinoide/uso terapêutico
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


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Id: lil-495754
Autor: Cifuentes, Mariana; Albala, Cecilia; Rojas, Cecilia V.
Título: Differences in lipogenesis and lipolysis in obese and non-obese adult human adipocytes
Fonte: Biol. Res;41(2):197-204, 2008. graf.
Idioma: en.
Projeto: U de Chile. DI; . FONDECYT.
Resumo: It has been proposed that differences in adipocyte function and/or metabolism between obese and lean individuáis may manifest themselves in functional adipose tissue abnormalities that lead to metabolic disorders in obesity. We studied lipogenesis and lipolysis of omental adipocytes from obese (OB) and non-obese (NOB) humans. The specific activity of the lipogenic marker enzyme G3PDH was 50 percent lower in total adipocytes of OB compared to that of NOB subjects. Omental adipocytes from OB subjects also had lower basal lipolytic levéis, and a lower lipolytic response to p-adrenergic stimulus. Cholesterol depletion of adipocyte plasma membrane using methyl β-cyclodextrin caused a lipolytic effect on adipocytes of both groups together, but when obese and lean subjects were analyzed separately, the response was significant only in the obese. We present evidence of a different lipogenic and lipolytic profile in obese individuáis' omental adipocytes, and propose a relevant role of plasma membrane cholesterol, where the impact of its removal in OB and NOB adipocyte lipolysis differs.
Descritores: Adipócitos/citologia
Lipogênese/fisiologia
Lipólise/fisiologia
Obesidade/fisiopatologia
Omento/citologia
-Adipócitos/fisiologia
Colesterol/metabolismo
Colesterol/fisiologia
Lipídeos de Membrana/fisiologia
Obesidade/metabolismo
Limites: Adulto
Idoso
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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