Base de dados : LILACS
Pesquisa : G02.111.570.080.689 [Categoria DeCS]
Referências encontradas : 6 [refinar]
Mostrando: 1 .. 6   no formato [Detalhado]

página 1 de 1

  1 / 6 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1016090
Autor: Castro, Daniela E; Murguía-Romero, Miguel; Thomé, Patricia E; Peña, Antonio; Calderón-Torres, Marissa.
Título: Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: in silico search of regulatory sequences responsive to salt and nitrogen stress
Fonte: Electron. j. biotechnol;29:1-6, sept. 2017. graf, tab.
Idioma: en.
Projeto: DGAPA-UNAM.
Resumo: Background: During salt stress, the yeast Debaryomyces hansenii synthesizes tyrosine as a strategy to avoid the oxidation of proteins. Tyrosine reacts with nitrogen radicals to form 3-nitrotyrosine. 3-nitrotyrosine prevents the effects of associated oxidative stress and thus contributes to the high halotolerace of the yeast. However, the mechanism of how D. hansenii counteracts the presence of this toxic compound is unclear. In this work, we evaluated D. hansenii's capacity to assimilate 3-nitrotyrosine as a unique nitrogen source and measured its denitrase activity under salt stress. To identify putative genes related to the assimilation of 3-nitrotyrosine, we performed an in silico search in the promoter regions of D. hansenii genome. Results: We identified 15 genes whose promoters had binding site sequences for transcriptional factors of sodium, nitrogen, and oxidative stress with oxidoreductase and monooxygenase GO annotations. Two of these genes, DEHA2E24178g and DEHA2C00286g, coding for putative denitrases and having GATA sequences, were evaluated by RT-PCR and showed high expression under salt and nitrogen stress. Conclusions: D. hansenii can grow in the presence of 3-nitrotyrosine as the only nitrogen source and has a high specific denitrase activity to degrade 3-nitrotyrosine in 1 and 2 M NaCl stress conditions. The results suggest that given the lack of information on transcriptional factors in D. hansenii, the genes identified in our in silico analysis may help explain 3-nitrotyrosine assimilation mechanisms.
Descritores: Tirosina/análogos & derivados
Tirosina/metabolismo
Debaromyces/genética
Debaromyces/metabolismo
-Tirosina/genética
Transcrição Genética
Leveduras
Sequências Reguladoras de Ácido Nucleico
Regiões Promotoras Genéticas
Estresse Oxidativo
Reação em Cadeia da Polimerase em Tempo Real
Osmorregulação
Extremófilos
FRONTAL LOBE0
Nitrogênio/metabolismo
Responsável: CL1.1 - Biblioteca Central


  2 / 6 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1008685
Autor: Herrera Paz, Edwin Francisco.
Título: ADN, disco duro de la vida / DNA, life's hard drive
Fonte: Rev. méd. hondur;86(1/2):75-85, ene-. jul. 2018.
Idioma: es.
Resumo: A lo largo de los últimos dos siglos la medicina se vio nutrida con los descubrimientos bioquímicos que impulsaron el entendimiento de los mecanismos isiopatológicos y facilitó el desarrollo de la terapéutica. En cambio, en el presente siglo entramos a la era de la genómica y del "big data", por lo que el estudio de las funciones del ADN como dispositivo de almacenamiento de información es esencial para la comprensión de la nueva medicina genómica personalizada, de precisión. En la presente revisión, se analiza el ADN como un dispositivo informático con tres funciones: almacenamiento, expresión y transmisión de la información acumulada a lo largo de la ilogenia en forma de secuencias de nucleótidos. Se describe cada una de estas funciones comparándolas con la información manejada por una computadora o una sociedad, y se brindan ejemplos de patologías que surgen ante el fallo de alguna de las funciones. La revisión bibliográica es amplia e incluye los artículos más relevantes, tanto históricos como del estado del arte, correspondientes a cada tema...(AU)
Descritores: Sequências Reguladoras de Ácido Nucleico
Biologia Computacional
Doenças Genéticas Inatas
-Genômica
Genética Médica
Limites: Seres Humanos
Responsável: HN1.1 - Biblioteca Médica Nacional


  3 / 6 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-757064
Autor: Truszkowski, Martín; Moreno, Rodolfo P; Santos, Silvia N; Moreno, Guillermo E; Iolster, Thomas; Siaba Serrate, Alejandro; Landry, Luis; Ratto, María Elena; Rufach, Daniel; Fernández, Analía; Vassallo, Juan C; Buamscha, Daniel; Debaisi, Gustavo; Yulitta, Horacio.
Autor: Grupo de Investigación Clínica y Epidemiológica en Terapia Intensiva Pediátrica.
Título: Características de las residencias de terapia intensiva pediátrica de la República Argentina: Encuesta nacional / Characteristics of pediatric intensive care residency programs in Argentina: A national survey
Fonte: Arch. argent. pediatr;113(5):425-432, oct. 2015. graf, tab.
Idioma: es.
Resumo: Introducción. La residencia de terapia intensiva pediátrica (TIP) tiene pocos años de desarrollo en nuestro país. Conocer su situación brinda la posibilidad de establecer estrategias para contribuir al desarrollo y capacitación de profesionales. Objetivos. 1) Describir las características de las residencias de TIP del país. 2) Evaluar si existen características que se relacionen con una mayor ocupación de las vacantes. 3) Explorar la inserción laboral en el hospital formador de los residentes. Diseño. Descriptivo, observacional. Encuesta nacional. Criterios de inclusión. Residencias de TIP funcionales entre el 1/4/2014 y el 31/5/2014. Resultados. Se analizaron 31 residencias. Solo 11/31 tenían volumen de internación anual >400 pacientes. No había normas y/o criterios de atención en 9/31. En 17/31, el programa estuvo adecuado al marco de referencia nacional. Hubo 13/31 que no contaban con jefe ni instructor de residentes. Fueron acreditadas por el Ministerio de Salud 5/31. Hubo 65 vacantes; el número aumentó en los últimos 4 años; la ocupación disminuyó de 59% en 2009 a 30% en 2013. El 60% de los residentes tuvo inserción laboral en la TIP formadora. El análisis de regresión logística multivariado identificó la variable ingresos anuales > 400 pacientes como predictora independiente de ocupación de vacantes > 60%. Conclusiones. 1) Hay un déficit en la ocupación de cargos. 2) El número de residencias acreditadas es escaso. 3) Las unidades de cuidados intensivos pediátricos con mayor número de ingresos se asociaron a una mayor cobertura de vacantes. 4) Más de la mitad de los residentes se insertaron laboralmente en la TIP formadora.

Introduction. Pediatric intensive care residency programs have been in place in Argentina for just a few years. Knowing their status offers the possibility to establish strategies to help with professional development and training. Objectives. 1) To describe the characteristics of pediatric intensive care residency programs across Argentina. 2) To assess whether certain characteristics are related to a higher vacancy filling rate. 3) To assess job placement in the hospital where residents are trained. Design. Descriptive, observational study. National survey. Inclusion criteria. Pediatric intensive care residency programs in place between April 1st, 2014 and May 31st, 2014. Results. Thirty-one residency programs were analyzed. Only 11/31 had an annual hospitalization volume >400patients. There were no guidelines and/or criteria for care in 9/31. The program suited the national reference frameworkin17/31. There was no head ofresidents or resident trainer in 13/31. Only 5/31 had been certified by the Ministry of Health. There were 65 vacancies; this number increased in the past four years; vacancy filling rate decreased from 59% in 2009 to 30% in 2013. Sixty percent of residents got a job in the pediatric intensive care unit where they were trained. A multivariate logistic regression analysis identified the outcome measure annual hospitalization volume >400 patients as an independent predictor of vacancy filling rate >60%. Conclusions. 1) Vacancy filling is deficient. 2) The number of certified residency programs is scarce. 3) Pediatric intensive care units with a higher number of hospitalizations were associated with a higher vacancy filling rate. 4) More than half of residents got a job in the pediatric intensive care unit where they were trained.
Descritores: Clonagem Molecular
Dioxigenases/genética
Frutas/genética
Expressão Gênica
Malus/genética
Proteínas de Plantas/genética
Estresse Fisiológico/genética
-Sequência de Aminoácidos
Mapeamento Cromossômico
Dioxigenases/química
Frutas/crescimento & desenvolvimento
Regulação da Expressão Gênica de Plantas
Íntrons
Dados de Sequência Molecular
Malus/classificação
Malus/crescimento & desenvolvimento
Filogenia
Regiões Promotoras Genéticas
Proteínas de Plantas/química
Sequências Reguladoras de Ácido Nucleico
Alinhamento de Sequência
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


  4 / 6 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-520060
Autor: Wanderley-Nogueira, A. C; Soares-Cavalcanti, N. M; Morais, D. A. L; Belarmino, L. C; Barbosa-Silva, A; Benko-Iseppon, A. M.
Título: Abundance and diversity of resistance genes in the sugarcane transcriptome revealed by in silico analysis
Fonte: Genet. mol. res. (Online);6(4):866-889, 2007. ilus, graf, tab.
Idioma: en.
Conferência: Apresentado em: X-Meeting 2006 - International Conference of the AB3C, 2, Apresentado em: Annual International Conference on Intelligent Systems for Molecular Biology, 14, Fortaleza, Aug. 6-10, 2006.
Resumo: Resistance genes (R-genes) are responsible for the first interaction of the plant with pathogens being responsible for the activation (or not) of the defense response. Despite their importance and abundance, no tools for their automatic annotation are available yet. The present study analyzed R-genes in the sugarcane expressed sequence tags database which includes 26 libraries of different tissues and development stages comprising 237,954 expressed sequence tags. A new annotation routine was used in order to avoid redundancies and overestimation of R-gene number, common mistakes in previous evaluations. After in silico screening, 280 R-genes were identified, with 196 bearing the complete domains expected. Regarding the alignments, most of the sugarcane's clusters yielded best matches with proteins from Oryza sativa, probably due to the prevalence of sequences of this monocot in data banks. All R-gene classes were found except the subclass LRR-NBS-TIR (leucine-rich repeats, nucleotide-binding site, including Toll interleukin-1 receptors), with prevalence of the kinase (Pto-like) class. R-genes were expressed in all libraries, but flowers, transition root to shoot, and roots were the most representative, suggesting that in sugarcane the expression of R-genes in non-induced conditions prevails in these tissues. In leaves, only low level of expression was found for some gene classes, while others were completely absent. A high allelic diversity was found in all classes of R-genes, sometimes showing best alignments with dicotyledons, despite the great number of genes from rice, maize and other grasses deposited in data banks. The results and future possibilities regarding R-genes in sugarcane research and breeding are further discussed.
Descritores: Biologia Computacional/métodos
Doenças das Plantas/genética
Perfilação da Expressão Gênica
Genes de Plantas
Variação Genética
Imunidade Inata/genética
Saccharum/genética
-Análise por Conglomerados
Etiquetas de Sequências Expressas
Regulação da Expressão Gênica de Plantas
Biblioteca Gênica
Filogenia
Sequências Reguladoras de Ácido Nucleico
Saccharum/enzimologia
Responsável: BR26.1 - Biblioteca Central


  5 / 6 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO México
Texto completo
Id: lil-332934
Autor: León, Natalia Manzano.
Título: Mecanismo de acción de las proteínas que se unen a los elementos regulatorios de esteroles (SREBPs) en la biosÝntesis del colesterol y ßcidos grasos / Mechanism of action of sterol regulatory element binding proteins (SREBPs) in cholesterol and fatty-acid biosynthesis
Fonte: Rev. invest. clín;54(2):145-153, Mar.-Abr. 2002.
Idioma: es.
Resumo: Cholesterol is an important lipid in higher organisms, and its concentration must be maintained in narrow limits depending of the cell needs. An excess of dietary cholesterol can lead to serious health problems, however, if consumption of this lipid is restricted in the diet, cells have the capacity to synthesize it. For the synthesis of cholesterol, the cell uses a family of proteins named sterol regulatory element binding proteins (SREBP's), that are transcriptional factors involved in the control of expression of genes of cholesterol and fatty acids synthesis. SREBP's regulate gene transcription by binding to cis-acting elements denominated sterol regulatory elements (SRE-1). SREBP's are localized in the endoplasmic reticulum, but in the event that the cell needs to synthesize cholesterol, the NH2-terminal portion of these proteins is cleaved by two specific proteases, and then travels into the nucleus to function as transcriptional factor. The present review shows the details of the mechanism that the cell uses to regulate cholesterol biosynthesis by the SREBP's, and its potential metabolic implications.
Descritores: Ácidos Graxos/biossíntese
Colesterol
Proteínas de Ligação a DNA
Fatores de Transcrição/fisiologia
Proteínas Estimuladoras de Ligação a CCAAT/fisiologia
Regulação da Expressão Gênica/fisiologia
-Colesterol na Dieta
Proteínas de Ligação a DNA
Fatores de Transcrição/genética
Genes
Insulina
Isoformas de Proteínas/fisiologia
Camundongos Mutantes
Proteínas de Membrana/fisiologia
Proteínas Estimuladoras de Ligação a CCAAT/genética
Sequências Reguladoras de Ácido Nucleico
Limites: Animais
Cricetinae
Seres Humanos
Camundongos
Responsável: BR1.1 - BIREME


  6 / 6 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-325035
Autor: Busek, S. U; Fantappie, M; Malaquias, L. C; Wilson, R. A; Corrêa-Oliveira, R; Oliveira, G. C.
Título: Cis-acting elements, CArG-, E-, CCAAT- and TATA-boxes may be involved in sexually regulated gene transcription in Schistosoma mansoni
Fonte: Mem. Inst. Oswaldo Cruz;97(suppl.1):85-90, Oct. 2002. ilus, tab, graf.
Idioma: en.
Conferência: Apresentado em: International Symposium on Schistosomiasis, 8, Recife, Dec. 2-5, 2001.
Resumo: Schistosomes undergo various morphological and metabolic changes during their development, reflected in a finely tuned regulation of protein and/or gene expression. The mechanisms involved in the control of gene expression during the development of the parasite are not understood. Two actin genes had been previously cloned and observed to be differentially expressed during the maturation of the parasite. The SmAct gene contains four putative cis-regulatory elements (TATA-, CCAAT-, E- and CArG-boxes). Our objective was to investigate in greater detail the expression pattern of two actin genes and verify if the binding of nuclear proteins to the promoter elements of SmAct correlated with the expression profile observed. We detected little variation in the expression of actin genes during the first seven days of schistosomula culture in vitro. However, we observed significantly higher levels of expression in males compared to female adults. CArG and CCAAT elements bound to a greater extent and formed distinct complexes with male in comparison to female nuclear extracts. In contrast, female extracts bound weakly to the E-box probe while no binding was observed with male extracts. Taken together these results describe cis-acting elements that appear to be involved in sexually regulated gene expression in Schistosoma mansoni
Descritores: Proteínas de Ligação a DNA
Regulação da Expressão Gênica
Regiões Promotoras Genéticas
Schistosoma mansoni
-Sequência de Bases
Northern Blotting
Proteínas Estimuladoras de Ligação a CCAAT
Dados de Sequência Molecular
Proteínas Nucleares
Sequências Reguladoras de Ácido Nucleico
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Mensageiro
Schistosoma mansoni
Proteína de Ligação a TATA-Box/análise
Fatores de Transcrição
Limites: Animais
Masculino
Feminino
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



página 1 de 1
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde