Base de dados : LILACS
Pesquisa : G02.111.570.080.708 [Categoria DeCS]
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Id: lil-769825
Autor: Cavalcanti, Felipe Lira de Sá; Mirones, Cristina Rodríguez; Paucar, Elena Román; Montes, Laura Álvarez; Leal-Balbino, Tereza Cristina; Morais, Marcia Maria Camargo de; Martínez-Martínez, Luis; Ocampo-Sosa, Alain Antonio.
Título: Mutational and acquired carbapenem resistance mechanisms in multidrug resistant Pseudomonas aeruginosa clinical isolates from Recife, Brazil
Fonte: Mem. Inst. Oswaldo Cruz;110(8):1003-1009, Dec. 2015. tab, graf.
Idioma: en.
Projeto: REIPI; . SNS Miguel Servet.
Resumo: An investigation was carried out into the genetic mechanisms responsible for multidrug resistance in nine carbapenem-resistant Pseudomonas aeruginosaisolates from different hospitals in Recife, Brazil. Susceptibility to antimicrobial agents was determined by broth microdilution. Polymerase chain reaction (PCR) was employed to detect the presence of genes encoding β-lactamases, aminoglycoside-modifying enzymes (AMEs), 16S rRNA methylases, integron-related genes and OprD. Expression of genes coding for efflux pumps and AmpC cephalosporinase were assessed by quantitative PCR. The outer membrane proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The blaSPM-1, blaKPC-2 and blaGES-1 genes were detected in P. aeruginosaisolates in addition to different AME genes. The loss of OprD in nine isolates was mainly due to frameshift mutations, premature stop codons and point mutations. An association of loss of OprD with the overexpression of MexAB-OprM and MexXY-OprM was observed in most isolates. Hyper-production of AmpC was also observed in three isolates. Clonal relationship of the isolates was determined by repetitive element palindromic-PCR and multilocus sequence typing. Our results show that the loss of OprD along with overexpression of efflux pumps and β-lactamase production were responsible for the multidrug resistance in the isolates analysed.
Descritores: Carbapenêmicos/metabolismo
Farmacorresistência Bacteriana Múltipla/genética
Mutação
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/genética
Resistência beta-Lactâmica/genética
beta-Lactamases/metabolismo
-Aminoglicosídeos/metabolismo
Anfotericina B/análogos & derivados
Anfotericina B/metabolismo
Antifúngicos/metabolismo
Brasil
Cefalosporinase/classificação
Cefalosporinase/metabolismo
Códon sem Sentido/metabolismo
Ativação Enzimática/genética
Mutação da Fase de Leitura/genética
Regulação Bacteriana da Expressão Gênica/genética
Proteínas de Membrana Transportadoras/metabolismo
Metiltransferases/metabolismo
Nucleotidiltransferases/metabolismo
Mutação Puntual/genética
Porinas/metabolismo
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/isolamento & purificação
Sequências Repetitivas de Ácido Nucleico
beta-Lactamases/genética
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-759340
Autor: Torres, Rosângela S.L.A.; Santos, Talita Z.; Torres, Robson A.A.; Pereira, Valéria V.G.; Fávero, Lucas A.F.; M. Filho, Otavio R.; Penkal, Margareth L.; Araujo, Leni S..
Título: Resurgence of pertussis at the age of vaccination: clinical, epidemiological, and molecular aspects / Ressurgimento da coqueluche na era vacinal: aspectos clínicos, epidemiológicos e moleculares
Fonte: J. pediatr. (Rio J.);91(4):333-338, July-Aug. 2015. tab, ilus.
Idioma: en.
Resumo: OBJECTIVE: Report the incidence, epidemiology, clinical features, death, and vaccination status of patients with whooping cough and perform genotypic characterization of isolates of B. pertussis identified in the state of Paraná, during January 2007 to December 2013.METHODS: Cross-sectional study including 1,209 patients with pertussis. Data were obtained through the Notifiable Diseases Information System (Sistema de Informação de Agravos de Notificação - SINAN) and molecular epidemiology was performed by repetitive sequence-based polymerase chain reaction (rep-PCR; DiversiLab(r), bioMerieux, France).RESULTS: The incidence of pertussis in the state of Paraná increased sharply from 0.15-0.76 per 100,000 habitants between 2007-2010 to 1.7-4.28 per 100,000 between 2011-2013. Patients with less than 1 year of age were more stricken (67.5%). Fifty-nine children (5%) developed pertussis even after receiving three doses and two diphtheria-tetanus-pertussis (DTP) boosters vaccine. The most common complications were pneumonia (14.5%), otitis (0.9%), and encephalopathy (0.7%). Isolates of B. pertussis were grouped into two groups (G1 and G2) and eight distinct patterns (G1: P1-P5 and G2: P6-P8).CONCLUSION: The resurgence of pertussis should stimulate new research to develop vaccines with greater capacity of protection against current clones and also encourage implementation of new strategies for vaccination in order to reduce the risk of disease in infants.

OBJETIVO: Relatar a incidência, os aspectos epidemiológicos, clínicos, a morte e a vacinação de pacientes com coqueluche e fazer a caracterização genotípica de isolados de Bordetella pertussisidentificados no Estado do Paraná, de janeiro de 2007 a dezembro de 2013.MÉTODOS: Estudo transversal, incluindo 1.209 pacientes com coqueluche. Os dados foram obtidos no Sistema de Informação de Agravos de Notificação (Sinan) e a epidemiologia molecular foi feita por PCR baseada em sequências repetitivas (rep-PCR; DiversiLab(r), bioMerieux, France).RESULTADOS: A incidência de coqueluche no Estado do Paraná aumentou acentuadamente de 0,15-0,76 por 100.000 habitantes entre 2007-2010 para 1,7-4,28 por 100.000 habitantes entre 2011-2013. Os pacientes com menos de um ano foram os mais afetados (67,5%); 59 crianças (5%) desenvolveram coqueluche mesmo depois de receber três doses da vacina e dois reforços com a vacina tríplice DTP. As complicações mais comuns foram pneumonia (14,5%), otite (0,9%) e encefalopatia (0,7%). Isolados de B. pertussis foram agrupados em dois grupos (G1 e G2) e oito padrões distintos (G1: P1-P5 e G2: P6-P8).CONCLUSÃO: O ressurgimento da coqueluche vem para sugerir novas pesquisas com o objetivo se desenvolverem vacinas com maior capacidade de proteção contra os clones atuais e também implantar novas estratégias de vacinação, a fim de reduzir o risco de doenças em lactentes.
Descritores: Bordetella pertussis/genética
Vacina contra Difteria, Tétano e Coqueluche/administração & dosagem
Vacinação/estatística & dados numéricos
Coqueluche/epidemiologia
-Distribuição por Idade
Bordetella pertussis/isolamento & purificação
Brasil/epidemiologia
Estudos Transversais
Cianose/complicações
Hospitalização/estatística & dados numéricos
Esquemas de Imunização
Incidência
Pneumonia/complicações
Sequências Repetitivas de Ácido Nucleico
Análise de Sequência de DNA
Coqueluche/complicações
Coqueluche/prevenção & controle
Limites: Adolescente
Adulto
Idoso
Criança
Pré-Escolar
Feminino
Seres Humanos
Lactente
Recém-Nascido
Masculino
Meia-Idade
Responsável: BR1.1 - BIREME


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Id: lil-661645
Autor: Peixoto, Juelto C. Fernandes; Santos, Emmanuel Aguiar; Paulino, Antônio Trindade; Faria, Carolina Gonçalves Pinho Piana de; Borlot, Paulo Estevão Wandekoken; Santos, Sávio Silva; Gomes, Andréia Patrícia.
Título: Aspectos etiopatogênicos da Doença de Huntington / Etiopathogenic aspects of Huntington's disease
Fonte: J. bras. med;88(3):47-51, mar. 2005.
Idioma: pt.
Resumo: A doença de Huntington (DH) é um distúrbio hereditário autossômico dominante, que está relacionado à expansão das repetições de CAG (citosina-adenina-guanina) no braço curto do cromossomo 4, o que leva à formação de uma proteína mutante associada, principalmente, à destruição neuronal do estriado. Manifesta-se por transtornos motores, cognitivos e neuropsicológicos, evoluindo progressivamente para estado demencial grave. A patogênese da doença ainda apresenta pontos obscuros. No entanto, recentes investigações têm possibilitado maior entendimento de sua origem e evolução, assim como de outras doenças neurodegenerativas

Huntington's disease is a hereditary autosomal dominant disorder which occurs due to the expansion of the repetitions CAG on the short arm of chromosome 4, which leads to the formation of a mutant protein itself associated principally to the destruction of neuronal of the striated tissue. It manifests through motor, cognitive and neuropsychological disorders where it evolves progressively to a serious demential state. The pathogenesis of this disease still presents obscure points although recent investigations made it possible to understand it better in its origin and evolution, the same as with other neurodegenerative diseases
Descritores: /genética
CROMOSSOMOS HUMANOS PAR ABBREVIATIONS AS TOPIC/genética
Doença de Huntington/etiologia
Doença de Huntington/genética
Doença de Huntington/patologia
Proteínas Mutantes/genética
Sequências Repetitivas de Ácido Nucleico
-Doenças Neurodegenerativas/etiologia
Degeneração Neural
Proteínas do Tecido Nervoso
Neurônios/patologia
Repetições de Trinucleotídeos/genética
Limites: Seres Humanos
Masculino
Feminino
Responsável: BR1365.1 - Biblioteca Biomédica A - CB/A


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Lemos, Elba Regina Sampaio de
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Id: lil-643760
Autor: Rozental, Tatiana; Mascarenhas, Luis Filipe; Rozenbaum, Ronaldo; Gomes, Raphael; Mattos, Grasiely Souza; Magno, Cecília Carlos; Almeida, Daniele Nunes; Rossi, Maria Inês Doria; Favacho, Alexsandra RM; Lemos, Elba Regina Sampaio de.
Título: Coxiella burnetii, the agent of Q fever in Brazil: its hidden role in seronegative arthritis and the importance of molecular diagnosis based on the repetitive element IS1111 associated with the transposase gene
Fonte: Mem. Inst. Oswaldo Cruz;107(5):695-697, Aug. 2012.
Idioma: en.
Resumo: Coxiella burnetii is the agent of Q fever , an emergent worldwide zoonosis of wide clinical spectrum. Although C. burnetii infection is typically associated with acute infection, atypical pneumonia and flu-like symptoms, endocarditis, osteoarticular manifestations and severe disease are possible, especially when the patient has a suppressed immune system; however, these severe complications are typically neglected. This study reports the sequencing of the repetitive element IS1111 of the transposase gene of C. burnetii from blood and bronchoalveolar lavage (BAL) samples from a patient with severe pneumonia following methotrexate therapy, resulting in the molecular diagnosis of Q fever in a patient who had been diagnosed with active seronegative polyarthritis two years earlier. To the best of our knowledge, this represents the first documented case of the isolation of C. burnetii DNA from a BAL sample.
Descritores: Artrite/microbiologia
Coxiella burnetii/genética
DNA Bacteriano/genética
Febre Q/diagnóstico
Sequências Repetitivas de Ácido Nucleico/genética
Transposases/genética
-Doença Aguda
Lavagem Broncoalveolar
Coxiella burnetii/isolamento & purificação
Limites: Adulto
Seres Humanos
Masculino
Tipo de Publ: Relatos de Casos
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-634551
Autor: Morcillo, N.; Zumarraga, M.; Imperiale, B.; Di Giulio, B.; Chirico, C.; Kuriger, A.; Alito, A.; Kremer, K.; Cataldi, A..
Título: Tuberculosis transmission of predominant genotypes of Mycobacterium tuberculosis in Northern suburbs of Buenos Aires city region / Transmisión de la tuberculosis por genotipos predominantes de Mycobacterium tuberculosis en la región Gran Buenos Aires Norte
Fonte: Rev. argent. microbiol;39(3):145-150, jul.-sep. 2007. ilus, tab.
Idioma: en.
Projeto: Roemmers Foundation Argentina; . European Commission. ICA4-CT-2001-10087.
Resumo: In 2003, the incidence of tuberculosis in Argentina showed an increase compared to 2002. The severe national crisis at the end of the 90s has probably strongly contributed to this situation. The goal of this work was to estimate the extent of the spread of the most predominant Mycobacterium tuberculosis strains and to assess the spread of predominant M. tuberculosis clusters as determined by spoligotyping and IS6110 RFLP. The study involved 590 pulmonary, smear-positive TB cases receiving medical attention at health centers and hospitals in Northern Buenos Aires (NBA) suburbs, from October 2001 to December 2002. From a total of 208 clinical isolates belonging to 6 major clusters, 63 (30.2%) isolates had identical spoligotyping and IS6110 RFLP pattern. Only 22.2% were shown to have epidemiological connections with another member of their respective cluster. In these major clusters, 30.2% of the 208 TB cases studied by both molecular techniques and contact tracing could be convincingly attributable to a recently acquired infection. This knowledge may be useful to assess the clonal distribution of predominant M. tuberculosis clusters in Argentina, which may make an impact on TB control strategies.

La incidencia de la tuberculosis en Argentina mostró en 2003 un incremento en comparación con 2002. La grave crisis nacional a fines de los 90 ha probablemente contribuido en gran medida a esta situación. El objetivo del presente trabajo fue determinar la diversidad genética de aislamientos de Mycobacterium tuberculosis y el grado de dispersión de algunas cepas mayoritarias genéticamente relacionadas. El estudio involucró 590 aislamientos clínicos provenientes de muestras respiratorias con examen directo positivo, de pacientes atendidos en los hospitales y centros de salud que conforman la región Gran Buenos Aires Norte (NBA), de octubre de 2001 a diciembre de 2002. De 208 aislamientos que se encontraron en los 6 mayores clusters, 63 (30,2%) tenían patrones idénticos de spoligotyping y de IS6110 RFLP. En el 22,2% de los casos fue posible verificar la conexión epidemiológica con otro miembro del respectivo cluster. Concluimos que el 30,2% de estos agrupamientos principales pueden ser atribuidos a una infección reciente. Estos resultados pueden ser útiles para determinar la distribución clonal de los grupos predominantes de M. tuberculosis en Argentina, lo que puede impactar en las estrategias de control de la tuberculosis.
Descritores: Transmissão de Doença Infecciosa
Mycobacterium tuberculosis/genética
Tuberculose/microbiologia
-Argentina/epidemiologia
Técnicas de Tipagem Bacteriana/métodos
Análise por Conglomerados
Elementos de DNA Transponíveis/genética
DNA Bacteriano/genética
Genótipo
Pessoal de Saúde
Infecções por HIV/epidemiologia
Incidência
Mycobacterium tuberculosis/classificação
Mycobacterium tuberculosis/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Sequências Repetitivas de Ácido Nucleico
População Suburbana
Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia
Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
Tuberculose Resistente a Múltiplos Medicamentos/transmissão
Tuberculose/epidemiologia
Tuberculose/transmissão
Limites: Adulto
Criança
Feminino
Seres Humanos
Masculino
Tipo de Publ: Estudo Multicêntrico
Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-597710
Autor: Varma-Basil, Mandira; Kumar, Sujeet; Arora, Jyoti; Angrup, Archana; Zozio, Thierry; Banavaliker, Jayant Nagesh; Singh, Urvashi Balbir; Rastogi, Nalin; Bose, Mridula.
Título: Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic diversity of Mycobacterium tuberculosis in Delhi, North India
Fonte: Mem. Inst. Oswaldo Cruz;106(5):524-535, Aug. 2011.
Idioma: en.
Projeto: ERDF/FEDER; . Regional Council of Guadeloupe. Biodiversity project, CR08/031380.
Resumo: The aim of the present study was to compare polymerase chain reaction (PCR)-based methods - spoligotyping and mycobacterial interspersed repetitive units (MIRU) typing - with the gold-standard IS6110 restriction fragment length polymorphism (RFLP) analysis in 101 isolates of Mycobacterium tuberculosis to determine the genetic diversity of M. tuberculosis clinical isolates from Delhi, North India. Spoligotyping resulted in 49 patterns (14 clusters); the largest cluster was composed of Spoligotype International Types (SITs)26 [Central-Asian (CAS)1-Delhi lineage], followed by SIT11 [East-African-Indian (EAI) 3-Indian lineage]. A large number of isolates (75 percent) belonged to genotypic lineages, such as CAS, EAI and Manu, with a high specificity for the Indian subcontinent, emphasising the complex diversity of the phylogenetically coherent M. tuberculosis in North India. MIRU typing, using 11 discriminatory loci, was able to distinguish between all but two strains based on individual patterns. IS6110-RFLP analysis (n = 80 strains) resulted in 67 unique isolates and four clusters containing 13 strains. MIRUs discriminated all 13 strains, whereas spoligotyping discriminated 11 strains. Our results validate the use of PCR-based molecular typing of M. tuberculosis using repetitive elements in Indian isolates and demonstrate the usefulness of MIRUs for discriminating low-IS6110-copy isolates, which accounted for more than one-fifth of the strains in the present study.
Descritores: DNA Bacteriano
Variação Genética
Repetições Minissatélites
Mycobacterium tuberculosis
-Técnicas de Tipagem Bacteriana
Análise por Conglomerados
Genótipo
Índia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Sequências Repetitivas de Ácido Nucleico
Limites: Adulto
Feminino
Seres Humanos
Masculino
Adulto Jovem
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Romanha, Alvaro José
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Id: lil-554803
Autor: Bahia, Diana; Rodrigues, Nilton B; Araújo, Flávio Marcos G; Romanha, Álvaro José; Ruiz, Jerônimo C; Johnston, David A; Oliveira, Guilherme.
Título: CA88, a nuclear repetitive DNA sequence identified in Schistosoma mansoni, aids in the genotyping of nine Schistosoma species of medical and veterinary importance
Fonte: Mem. Inst. Oswaldo Cruz;105(4):391-397, July 2010. tab, ilus.
Idioma: en.
Projeto: FAPESP; . WHO. TDR; . NIH-Fogarty.
Resumo: CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma mansoni. The assembled S. mansoni sequence, which contains the CA88 repeat, has 8,887 nucleotides and at least three repeat units of approximately 360 bp. In addition, CA88 also possesses an internal CA microsatellite, identified as SmBr18. Both PCR and BLAST analysis have been used to analyse and confirm the CA88 sequence in other S. mansoni sequences in the public database. PCR-acquired nuclear repetitive DNA sequence profiles from nine Schistosoma species were used to classify this organism into four genotypes. Included among the nine species analysed were five sequences of both African and Asian lineages that are known to infect humans. Within these genotypes, three of them refer to recognised species groups. A panel of four microsatellite loci, including SmBr18 and three previously published loci, has been used to characterise the nine Schistosoma species. Each species has been identified and classified based on its CA88 DNA fingerprint profile. Furthermore, microsatellite sequences and intra-specific variation have also been observed within the nine Schistosoma species sequences. Taken together, these results support the use of these markers in studying the population dynamics of Schistosoma isolates from endemic areas and also provide new methods for investigating the relationships between different populations of parasites. In addition, these data also indicate that Schistosoma magrebowiei is not a sister taxon to Schistosoma mattheei, prompting a new designation to a basal clade.
Descritores: DNA de Helmintos
Repetições de Microssatélites
Sequências Repetitivas de Ácido Nucleico
Schistosoma
-Impressões Digitais de DNA
Genótipo
Filogenia
Reação em Cadeia da Polimerase
Schistosoma mansoni
Schistosoma
Limites: Animais
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-504118
Autor: Hakki, Erdogan E; Kayis, Seyit A; Pinarkara, Emine; Sag, Ayla.
Título: Inter simple sequence repeats separate efficiently hemp from marijuana ( Cannabis sativa L. )
Fonte: Electron. j. biotechnol;10(4):570-581, oct. 2007. ilus, graf, tab.
Idioma: en.
Projeto: Turkish State Planning Organization; . The Scientific and Technical Research Council of Turkey; . The Scientific Research Coordination Center of Selcuk University.
Resumo: Cannabis sativa L. is a multiple-use plant that provides raw material for the production of seed oil, natural fiber for textiles, automotive and pulp industries. It has also been used in insulating boards, ropes, varnishes, animal feed, and as medicinal agents. Cannabis has potential to be used for phytoremediation: however, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 23 different locations of Turkey, and nine hemp type Cannabis accessions, as well as an unknown accession were used. Our interest was to identify the genetic relatedness of the seized samples and to separate drug and hemp type plants. Inter Simple Sequence Repeats (ISSRs) were employed for analysis based on single plant material (SET1) and bulked samples of them (SET2). Data was analysed via cluster analysis and principal coordinate analysis (PCoA). PCoA analyses, by using SET1 and SET2, were able to efficiently discriminate the seized samples from the fiber type accessions. However, separation of the plants was not clear via unweighted pair-group method using arithmetic average (UPGMA) dendogram in SET1, while they were clearly separated in SET2. Hemp type accessions showed high levels of variation compared to drug type Cannabis both in SET1 and SET2.
Descritores: Cannabis/genética
Primers do DNA
Variação Genética
Técnicas de Amplificação de Ácido Nucleico
Repetições de Microssatélites/genética
-Biologia Molecular/métodos
DNA de Plantas
Marcadores Genéticos
Reação em Cadeia da Polimerase
Polimorfismo Genético
Sequências Repetitivas de Ácido Nucleico/genética
Responsável: CL1.1 - Biblioteca Central


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Id: lil-501732
Autor: Cuenca, Patricia; Morales, Fernando.
Título: Las mutaciones inestables, nuevo reto para el consejo genético de enfermedades hereditarias: [revisión] / Unstable mutations, new challenges for genetic counseling of inherited disorders: [review]
Fonte: Rev. biol. trop;52(3):491-499, sept. 2004. tab.
Idioma: es.
Resumo: Unstable mutations or amplification of DNA tandem repeats sequences constitute a new kind of genetic alteration discovered in the 90's that cause hereditary diseases. This mutation has been found inside or near important genes involved in the normal neurological function in human beings. In some cases, the presence of the amplification causes altered expression of the genes, their inactivation or the synthesis of a protein with new functions. Some common characteristics of these diseases are that they affect the central nervous system and are degenerative in nature. Most of them show genetic anticipation meaning that the severity of the manifestations increases in each generation and appear at an earlier age. In most cases, the severity of the symptoms is positively correlated with the size of the amplification. Twenty illnesses caused by this kind of mutations have been identified so far. Briefly, this work reviews the current knowledge about this topic.
Descritores: Aconselhamento Genético
Mutação/genética
Sequências Repetitivas de Ácido Nucleico/genética
Transtornos Heredodegenerativos do Sistema Nervoso/genética
-Predisposição Genética para Doença
Valor Preditivo dos Testes
Transtornos Heredodegenerativos do Sistema Nervoso/diagnóstico
Limites: Seres Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Texto completo
Id: lil-456611
Autor: Mancuso, Monique; Avendaño-Herrera, Rubén; Zaccone, R; Toranzo, Alicia e; Magariños, Beatriz.
Título: Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
Fonte: Biol. Res;40(1):85-92, 2007. ilus, tab.
Idioma: en.
Projeto: Spain. Ministerio de Ciencia y Tecnología.
Resumo: This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections.
Descritores: Técnicas de Tipagem Bacteriana/métodos
Impressões Digitais de DNA/métodos
Variação Genética
Photobacterium/classificação
-DNA Bacteriano/genética
Peixes/microbiologia
Photobacterium/genética
Reação em Cadeia da Polimerase/métodos
Técnica de Amplificação ao Acaso de DNA Polimórfico
Sequências Repetitivas de Ácido Nucleico/genética
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde