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Id: lil-739227
Autor: Martínez Ortiz, Carlos M; Sautié Castellanos, Miguel; Cuza Ferrer, Yordanka; Wisdom Viña, Yinette.
Título: Herramienta web para la clasificación de microsatélites polimórficos en genomas bacterianos / Web Tool for classfication of polymorphic microsatellites in bacteria genomes
Fonte: Rev. cuba. inform. méd;5(1):20-29, ene.-jun. 2013.
Idioma: es.
Resumo: Las secuencias repetidas en tándem, específicamente los mini y micro satélites, han demostrado ser muy eficaces en la clasificación de bacterias patogénicas como B. anthracis, M. tuberculosis y P. aeruginosa, entre otras. En humanos es manifiesta su participación estando relacionados con más de ochenta enfermedades, gran parte de ellas de tipo neurodegenerativas, musculares y algunos tipos de cáncer. La herramienta web que presentamos es el resultado de la detección computacional de estas secuencias en genomas bacterianos completos y su correspondiente anotación en la estructura genómica de acuerdo a las diferentes regiones donde estos se localizan. La herramienta tiene como fin primario brindar un sistema relacional que permita al investigador ubicar los microsatélites de diferentes especies bacterianas, con más de un genoma secuenciado para inferir su posible carácter polimórfico, dentro del contexto de la estructura genómica y así proveer un primer acercamiento al rol putativo que los microsatélites desempeñan desde el punto de vista funcional. La herramienta se puede aplicar no solo en estudios taxonómicos y epidemiológicos sino en la detección de posibles relaciones de estas secuencias con las funciones moleculares, procesos biológicos y, en última instancia, las diversas formas de evolución de estas especies. El sitio web brinda el servicio de consultas a la base de datos de microsatélites bacterianos de acuerdo al sistema de tablas relacionales y atributos propios de las mismas. Cuenta además con los servicios típicos de un sitio con estas características como: sistema de autenticación, foro, encuestas, enlaces y documentación sobre la metodología empleada y del tema en cuestión(AU)

The tandem repeat sequences, especially mini and microsatellites, have proven to be very effective in classification of pathogenic bacteria such as B. anthracis, M. tuberculosis and P. aeruginosa, among others. In human beings it is manifest its participation, being related with over eighty diseases, nearly all neurodegenerative and muscular, and some kinds of cancer. The web tool we are offering here is the result of computational detection of these sequences in whole bacteria genomes, and its respective annotation in the genomic structure according to the different regions where they are localized. The primary goal of this tool is to offer a relational system that allows mapping the microsatellites of bacterial species, all of them with more than one genome sequenced to infer their possible polymorphic character, in the context of genomic structure and thus providing a first approach to the putative role they perform from the functional point of view. The tool can be applied not only in taxonomical and epidemiological studies but in the detection of possible relationships of these sequences with the molecular functions, the biological processes and, as a last resort, the different forms of these species evolution. The web site offers the service of queries to the bacterial microsatellites database according to the related tables and its inherent attributes. It also has the typical services of this kind of site like: logging system, forum, polls, links and documentation about the employed methodology and the topic(AU)
Descritores: Bactérias
Repetições de Microssatélites/genética
Sequências de Repetição em Tandem/genética
Bases de Dados Genéticas
Limites: Seres Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-834828
Autor: Moreno Valencia, Sandra P; Pineda Monsalve, Cielo R.
Título: Pruebas de paternidad mediante ADN / DNA paternity test
Fonte: Med. lab;20(9-10):411-432, 2014. tab, graf, ilus.
Idioma: es.
Resumo: La investigación científica de la paternidad biológica es un caso especial de la determinación de la relación genética entre dos o más individuos, con base en los principios de la herencia mendeliana simple de los marcadores genéticos, en los que se establecen que los alelos se segregan en la meiosis de forma independiente y discreta. En la evaluación de las relaciones biológicas forenses la prueba de paternidad es el análisis más común, en el cual los perfiles genéticos de dos individuos (o tres si la madre está disponible) son utilizados para comparar la probabilidad relativa de que uno de ellos sea el padre contra la probabilidad de no estar relacionado con la ascendencia del otro individuo analizado. En este caso las repeticiones cortas en tándem (STR) son los marcadores genéticos no ligados más utilizados para la evaluación del parentesco debido a su alto polimorfismo, y a que los resultados obtenidos, usualmente con la aplicación de estadísticas robustas, favorecen la determinación de la relación. Las repeticiones cortas en tándem son típicamente examinadas por la técnica de reacción en cadena de la polimerasa (PCR) mediante plataformas comerciales de múltiples loci de repeticiones cortas en tándem, eficientes y con gran poder de discriminación. Esta revisión describe el estado actual de las pruebas de paternidad mediante ADN, la metodología, el informe de los resultados y su interpretación, para facilitar su comprensión a los profesionales relacionados de una o de otra manera con estos análisis.

Scientific study of biological paternity is a special case of genetic relationship determinationbetween individuals, based on simple mendelian inheritance principles of genetic markers, which establish that the alleles are segregate independently and discreetly during meiosis. The most common analysis of forensic biological relationships evaluation is the paternity test, which the genetic profiles of two individuals (or three if the mother is available) are uses to compare the relative probability of that one of them being the father against the probability of not is ancestrally related to the other analyzed individual. The short tandem repeats (STR) are the commonly used unlinked genetic markers for relationship evaluation due to their high polymorphism. Besides, the results usually favor the determinationof the relationship when a robust statistical analysis is applied. The short tandem repeats analysis is typically by the polymerase chain reaction (PCR), using multiple short tandem repeats loci on commercial platforms, which are efficient and with high discriminating power. This review describes the current state of DNA paternity test, the methodology, the results report, and the interpretation, to facilitate their understanding to related professionals with these types of analysis.
Descritores: Testes Genéticos
Paternidade
Reação em Cadeia da Polimerase
Sequências de Repetição em Tandem
Limites: Seres Humanos
Tipo de Publ: Revisão
Responsável: CO373.9 - EDIMECO - Editora Médica Colombiana S.A.


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Aguiar, Paulo Ricardo Loss
Weimer, Tania de Azevedo
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Id: lil-789892
Autor: Silveira, Juliano Coelho da; Passos, Daniel Thompsen; Glanzner, Werner Giehl; Aguiar, Paulo Ricardo Loss; Moraes, José Carlos Ferrugem; Weimer, Tania de Azevedo.
Título: Genome association analysis for pregnancy status following parturition in crossbred beef cattle / Estudo de associação genômica de prenhez pós-parto em vacas de corte
Fonte: Braz. j. vet. res. anim. sci;50(5):406-413, 2013. tab.
Idioma: en.
Resumo: The aim of this study was to evaluate genetic diversity of nine molecular markers, six short tandem repeats - STRs (BM4325, BMS3004, ILSTS002, IDVGA51, HEL5, AFZ1) and three single nucleotide polymorphisms (SNPs; LepSau3A1 A-B, LepSau3A1 1-2, and FSHRAlu1), linked to genes involved in reproductive function and their possible effect on reproductive performance. For this purpose, 81 crossbred beef cows were used in this study. The animals were classified into two groups (fertile and sub-fertile cows) based on their pregnancy status after two breeding seasons. High genetic diversity level was observed highlighted by the polymorphic content information ranging 0.23 to 0.87 and expected heterozygosity from 27 to 89%, with an average of 62%. Alleles BM4325 103, BMS3004 129, ILSTS002 137, IDVGA51 177, LEPSau3A1 A, LEPSau3A1 1, HEL5 149, AFZ1 119 and FSHRAlu1 G presented high frequencies. Two STRs (IDVGA51 and ILSTS002), linked to Leptin and LH genes, respectively, were associated to reproductive performance. These data support previous findings suggesting the potential use of IDVGA51 and ILSTS002 STRs for reproductive performance selection.

Foi avaliada a diversidade genética de nove marcadores moleculares, dos quais seis do tipo short tandem repeats - STR (BM4325, BMS3004, ILSTS002, IDVGA51, HEL5, AFZ1) e três do tipo single nucleotide polymorphisms - SNPs (LepSau3A1 A-B, LepSau3A1 1-2 e FSHRAlu1), ligados a genes envolvidos na reprodução e seus efeitos na performance reprodutiva. Foram examinadas amostras de sangue de 81 vacas sem raça definida, os animais foram classificados em dois grupos (vacas férteis e subférteis) baseado nas taxas de prenhez de duas estações reprodutivas. Alto nível de diversidade genética foi observado, revelando alto conteúdo de informação polimórfica, variando de 0,23 a 0,87 e heterozigosidade esperada de 27 a 89% com 62% em média. Os alelos mais frequentes foram BM4325 103*, BMS3004 129*, ILSTS002 137*, IDVGA51 177*, LEPSau3A1 A, LEPSau3A1 1, HEL5 149*, AFZ1 119* e FSHRAlu1 G. Os marcadores IDVGA51 e ILSTS002, ligados aos genes da leptina e LH, respectivamente, foram associados a performance reprodutiva. Esses dados suportam achados prévios que sugerem o potencial uso desses marcadores na seleção de animais com maior performance reprodutiva.
Descritores: Hormônio Luteinizante Subunidade beta/genética
Leptina/genética
Polimorfismo de Nucleotídeo Único/genética
Sequências de Repetição em Tandem/genética
Variação Genética/genética
-Técnicas de Reprodução Assistida/veterinária
Limites: Animais
Feminino
Gravidez
Bovinos
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-747152
Autor: AlNoury, Mohammed K.; Almuhayawi, Saad M.; Alghamdi, Khalid B.; Al-Noury, Khaled I..
Título: Preoperative Imaging Modalities to Predict the Risk of Regional Nodal Recurrence in Well-Differentiated Thyroid Cancers
Fonte: Int. arch. otorhinolaryngol. (Impr.);19(2):116-120, Apr-Jun/2015. tab.
Idioma: en.
Resumo: Introduction Thyroid cancer incidence has increased in the previous 2 decades. Preoperative identification of lymph node metastasis is a suggested risk factor associated with recurrence following thyroidectomy. Objectives We aimed to evaluate the accuracy of preoperative radiologic investigations of nodal status in determining the postoperative risk of regional nodal recurrence in cases of well-differentiated thyroid cancer. Methods This is a case series. We retrospectively reviewed data, including preoperative ultrasonography and/or computed tomography results, on patients who underwent total thyroidectomy for thyroid cancer at our hospital between 2006 and 2012. Prognostic factors for predicting recurrence, including age, sex, tumor diameter, and nodal diameter, were evaluated. Results Total thyroidectomy was performed on 24 male and 74 female patients (median age, 43 years). The median follow-up time was 21 months. Sixty-eight patients had papillary thyroid cancer, and 30 had follicular cancer. Nodal recurrence was evident in 30% of patients, and 4% of patients died. Identification of lymph node involvement during preoperative radiologic investigations was strongly prognostic for recurrence: 35.3% of patients with positive preoperative ultrasonography findings and 62.5% of those with positive preoperative computed tomography findings had recurrence (p = 0.01). Conclusions Preoperative identification of lymph node metastasis on radiologic studies was correlated with an increased risk of regional nodal recurrence in well-differentiated thyroid cancer. Computed tomography was superior to ultrasonography in detecting metastatic nodal involvement preoperatively and is therefore recommended for preoperative assessment and postoperative follow-up. .
Descritores: Hematopoese/genética
Leucemia Mieloide Aguda/genética
Proteínas de Peixe-Zebra/fisiologia
Peixe-Zebra/fisiologia
/fisiologia
FMS-LIKE TYROSINE KINASE ABATTOIRS/fisiologia
-Sequência de Aminoácidos
Animais Geneticamente Modificados
Sequência Conservada
Embrião não Mamífero
Dados de Sequência Molecular
Estrutura Terciária de Proteína/genética
Homologia de Sequência de Aminoácidos
Sequências de Repetição em Tandem
Transcriptoma
Proteínas de Peixe-Zebra/química
Peixe-Zebra/embriologia
/química
FMS-LIKE TYROSINE KINASE ABATTOIRS/química
Limites: Animais
Seres Humanos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-725670
Autor: Merzoni, Jóice; Ewald, Gisele Menezes; Paz, Alessandra Aparecida; Daudt, Liane Esteves; Jobim, Luiz Fernando Job.
Título: Quantification of mixed chimerism allows early therapeutic interventions
Fonte: Rev. bras. hematol. hemoter;36(5):369-372, Sep-Oct/2014. tab.
Idioma: en.
Resumo: Hematopoietic stem cell transplantation is the curative option for patients with myelodysplastic syndrome; however, it requires a long post-transplantation follow-up. A 53-year-old woman with a diagnosis of myelodysplastic syndrome underwent related donor allogeneic hematopoietic stem cell transplantation in July 2006. Three months after transplantation, a comparative short tandem repeat analysis between donor and recipient revealed full chimerism, indicating complete, healthy bone marrow reconstitution. Three years and ten months after hematopoietic stem cell transplantation, the patient developed leukopenia and thrombocytopenia. Another short tandem repeat analysis was carried out which showed mixed chimerism (52.62%), indicating relapsed disease. A donor lymphocyte infusion was administered. The purpose of donor lymphocyte infusion is to induce a graft-versus-leukemia effect; in fact, this donor's lymphocyte infusion induced full chimerism. Successive short tandem repeat analyses were performed as part of post-transplantation follow-up, and in July 2010, one such analysis again showed mixed chimerism (64.25%). Based on this finding, a second donor lymphocyte infusion was administered, but failed to eradicate the disease. In September 2011, the patient presented with relapsed disease, and a second related donor allogeneic hematopoietic stem cell transplantation was performed. Subsequent short tandem repeat analyses revealed full chimerism, indicating complete bone marrow reconstitution. We conclude that quantitative detection of mixed chimerism is an important diagnostic tool that can guide early therapeutic intervention...
Descritores: Transplante de Medula Óssea
Quimerismo
Doenças Mieloproliferativas-Mielodisplásicas
Sequências de Repetição em Tandem
Limites: Seres Humanos
Tipo de Publ: Relatos de Casos
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-723983
Autor: Peñuelas-Urquides, Katia; Martínez-Rodríguez, Herminia Guadalupe; Enciso-Moreno, José Antonio; Molina-Salinas, Gloria María; Silva-Ramírez, Beatriz; Padilla-Rivas, Gerardo Raymundo; Vera-Cabrera, Lucio; Torres-de-la-Cruz, Víctor Manuel; Martínez-Martínez, Yazmin Berenice; Ortega-García, Jorge Luis; Garza-Treviño, Elsa Nancy; Enciso-Moreno, Leonor; Saucedo-Cárdenas, Odila; Becerril-Montes, Pola; Said-Fernández/, Salvador.
Título: Correlations between major risk factors and closely related Mycobacterium tuberculosis isolates grouped by three current enotyping procedures: a population-based study in northeast Mexico
Fonte: Mem. Inst. Oswaldo Cruz;109(6):814-819, 09/09/2014. tab, graf.
Idioma: en.
Projeto: SALUD.
Resumo: The characteristics of tuberculosis (TB) patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB) isolates (120) were genotyped using the restriction fragment length polymorphism-IS6110 (R), spacer oligotyping (S) and mycobacterial interspersed repetitive units-variable number of tandem repeats (M) methods. The MTB isolates were clustered and the clusters were grouped according to the similarities of their genotypes. Spearman’s rank correlation coefficients between the groups of MTB isolates with similar genotypes and those patient characteristics indicating a risk for a pulmonary TB (PTB) chain transmission were ana- lysed. The isolates showing similar genotypes were distributed as follows: SMR (5%), SM (12.5%), SR (1.67%), MR (0%), S (46.67%), M (5%) and R (0%). The remaining 35 cases were orphans. SMR exhibited a significant correlation (p < 0.05) with visits to clinics, municipalities and comorbidities (primarily diabetes mellitus). S correlated with drug consumption and M with comorbidities. SMR is needed to identify a social network in metropolitan areas for PTB transmission and S and M are able to detect risk factors as secondary components of a transmission chain of TB.
Descritores: Técnicas de Genotipagem/métodos
Mycobacterium tuberculosis/genética
Tuberculose Pulmonar/microbiologia
Tuberculose Pulmonar/transmissão
-Cidades
Comorbidade
DNA Bacteriano/isolamento & purificação
Genótipo
Sequências Repetitivas Dispersas/genética
Testes de Sensibilidade Microbiana
México/epidemiologia
Epidemiologia Molecular/métodos
Mycobacterium tuberculosis/classificação
Mycobacterium tuberculosis/isolamento & purificação
Polimorfismo de Fragmento de Restrição/genética
Fatores de Risco
Fatores Sociológicos
Estatísticas não Paramétricas
Sequências de Repetição em Tandem/genética
Tuberculose Pulmonar/epidemiologia
Tuberculose Pulmonar/genética
População Urbana
Limites: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-705823
Autor: Loffler, Sylvia Grune; Pavan, Maria Elisa; Vanasco, Bibiana; Samartino, Luis; Suarez, Olga; Auteri, Carmelo; Romero, Graciela; Brihuega, Bibiana.
Título: Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina
Fonte: Mem. Inst. Oswaldo Cruz;109(2):163-167, abr. 2014. tab.
Idioma: en.
Projeto: Proyecto Nacional INTA.
Resumo: Leptospirosis is the most widespread zoonosis in the world and significant efforts have been made to determine and classify pathogenic Leptospira strains. This zoonosis is maintained in nature through chronic renal infections of carrier animals, with rodents and other small mammals serving as the most important reservoirs. Additionally, domestic animals, such as livestock and dogs, are significant sources of human infection. In this study, a multiple-locus variable-number tandem repeat analysis (MLVA) was applied to genotype 22 pathogenic Leptospira strains isolated from urban and periurban rodent populations from different regions of Argentina. Three MLVA profiles were identified in strains belonging to the species Leptospira interrogans (serovars Icterohaemorrhagiae and Canicola); one profile was observed in serovar Icterohaemorrhagiae and two MLVA profiles were observed in isolates of serovars Canicola and Portlandvere. All strains belonging to Leptospira borgpetersenii serovar Castellonis exhibited the same MLVA profile. Four different genotypes were isolated from urban populations of rodents, including both mice and rats and two different genotypes were isolated from periurban populations.
Descritores: Leptospira/genética
Roedores/microbiologia
-Argentina
Didelphis/microbiologia
Genótipo
Técnicas de Genotipagem/métodos
Leptospira interrogans serovar canicola/genética
Leptospira interrogans serovar icterohaemorrhagiae/genética
Leptospira interrogans serovar pomona/genética
Leptospira/classificação
Leptospira/isolamento & purificação
Leptospirose/transmissão
Sorogrupo
Sorotipagem
Sequências de Repetição em Tandem/genética
População Urbana
Virulência/genética
Limites: Animais
Camundongos
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-676928
Autor: Coletta, Rocio R.D.; Jorge, Alexander A.L.; D'Alva, Catarina Brasil; Pinto, Emília M.; Billerbeck, Ana Elisa C.; Pachi, Paulo R.; Longui, Carlos A.; Garcia, Ricardo M.; Boguszewski, Margaret; Arnhold, Ivo J.P.; Mendonca, Berenice B.; Costa, Elaine M.F..
Título: Insulin-like growth factor 1 gene (CA)n repeats and a variable number of tandem repeats of the insulin gene in Brazilian children born small for gestational age
Fonte: Clinics;68(6):785-791, jun. 2013. tab.
Idioma: en.
Projeto: FAPESP; . FAPESP; . CNPq; . CNPq; . CNPq; . CNPq; . CAPES.
Resumo: OBJECTIVE: To investigate the influence of (CA)n repeats in the insulin-like growth factor 1 gene and a variable number of tandem repeats of the insulin gene on birth size in children who are small or adequate-sized for gestational age and to correlate these polymorphisms with serum insulin-like growth factor 1 levels and insulin sensitivity in children who are small for gestational age, with and without catch-up growth. PATIENTS AND METHODS: We evaluated 439 infants: 297 that were adequate-sized for gestational age and 142 that were small for gestational age (66 with and 76 without catch-up). The number of (CA)n repeat in the insulin-like growth factor 1 gene and a variable number of tandem repeats in the insulin gene were analyzed using GENESCAN software and polymerase chain reaction followed by enzymatic digestion, respectively. Clinical and laboratory data were obtained from all patients. RESULTS: The height, body mass index, paternal height, target height and insulin-like growth factor 1 serum levels were higher in children who were small for gestational age with catch-up. There was no difference in the allelic and genotypic distributions of both polymorphisms between the adequate-sized and small infants or among small infants with and without catch-up. Similarly, the polymorphisms were not associated with clinical or laboratory variables. CONCLUSION: Polymorphisms of the (CA)n repeats of the insulin-like growth factor 1 gene and a variable number of tandem repeats of the insulin gene, separately or in combination, did not influence pre- or postnatal growth, insulin-like growth factor 1 serum levels or insulin resistance. .
Descritores: Recém-Nascido Pequeno para a Idade Gestacional
Fator de Crescimento Insulin-Like I/genética
Insulina/genética
Polimorfismo Genético
Sequências de Repetição em Tandem/genética
-Adenosina
Brasil
Peso ao Nascer/genética
Glicemia/genética
Estatura/genética
Peso Corporal/genética
Citosina
Resistência à Insulina/genética
Fator de Crescimento Insulin-Like I/análise
Fatores de Risco
Limites: Feminino
Seres Humanos
Recém-Nascido
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Lage, Andrey Pereira
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Id: lil-612807
Autor: Parreiras, Patrícia Martins; Andrade, Giovanna Ivo; Nascimento, Telma de Figueiredo do; Oelemann, Maraníbia Cardoso; Gomes, Harrison Magdinier; Alencar, Andrea Padilha de; Assis, Ronnie Antunes de; Mota, Pedro Moacyr Pinto Coelho; Pereira, Márcia Aparecida da Silva; Lobato, Francisco Carlos Faria; Lage, Andrey Pereira; Suffys, Philip Noel.
Título: Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil
Fonte: Mem. Inst. Oswaldo Cruz;107(1):64-73, Feb. 2012. mapas, tab.
Idioma: en.
Resumo: We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs) typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs in Brazil. High-quality genotypes were obtained with both procedures for 61 isolates that were obtained from 185 bovine tissue samples and all of these isolates were identified as M. bovis by conventional identification procedures. On the basis of the spoligotyping, 53 isolates were grouped into nine clusters and the remaining eight isolates were unique types, resulting in 17 spoligotypes. The majority of the Brazilian M. bovis isolates displayed spoligotype patterns that have been previously observed in strains isolated from cattle in other countries. MIRU-VNTR typing produced 16 distinct genotypes, with 53 isolates forming eight of the groups, and individual isolates with unique VNTR profiles forming the remaining eight groups. The allelic diversity of each VNTR locus was calculated and only two of the 12-MIRU-VNTR loci presented scores with either a moderate (0.4, MIRU16) or high (0.6, MIRU26) discriminatory index (h). Both typing methods produced similar discriminatory indexes (spoligotyping h = 0.85; MIRU-VNTR h = 0.86) and the combination of the two methods increased the h value to 0.94, resulting in 29 distinct patterns. These results confirm that spoligotyping and VNTR analysis are valuable tools for studying the molecular epidemiology of M. bovis infections in Brazil.
Descritores: Técnicas de Tipagem Bacteriana/métodos
Variação Genética/genética
Mycobacterium bovis/genética
Sequências de Repetição em Tandem/genética
-Alelos
DNA Bacteriano/genética
Genótipo
Mycobacterium bovis/classificação
Mycobacterium bovis/isolamento & purificação
Limites: Animais
Bovinos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-537109
Autor: Zheng, Lihong; Sun, Haiming; Wang, Jingwei; Li, Shilin; Bai, Jing; Jin, Yan; Yu, Yang; Chen, Feng; Jin, Li; Fu, Songbin.
Título: Y Chromosomal STR Polymorphism in Northern Chinese Populations
Fonte: Biol. Res;42(4):497-504, 2009. mapas, tab, graf, ilus.
Idioma: en.
Resumo: Y chromosomal STRs show sufficient variability among individduals in a population and a high degree of geographical differentiation, such that their polymorphic character makes them especially suited for population genetic studies. To investígate the polymorphism of a set of 17 Y-STR loci in northern China, we genotyped the 17 Y chromosomal STR loci in a population sample of 377 unrelated males from eight ethnic populations in northern China. We calculated the haplotype frequencies, Rst value and carried out the analysis of molecular variance (AMOVA). We then drew the multidimensional scaling analysis (MDS) plot and phylogenetic tree based on the Rst value. All populations showed a high level of haplotype diversity, with low inter-population variance as measured by an analysis of molecular variance. However, the genetic distances were significant when the eight populations were compared to other populations. By MDS and the phylogenetic tree, we found that the eight populations had a close relationship and Xibo had a northeast origination.
Descritores: Cromossomos Humanos Y/genética
Grupos Étnicos/genética
Genética Populacional
Polimorfismo Genético/genética
Sequências de Repetição em Tandem/genética
-China/etnologia
Genótipo
Haplótipos
Filogenia
Limites: Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde