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Id: biblio-968986
Autor: kirouani, Abderrezzak; Henkrar, Fatima; Udupa, Sripada M; Boukhalfoun, Leila; Bouzerzour, Hamenna.
Título: Genetic diversity in algerian durum wheat varieties (Triticum turgidum L var. durum) using microsatellite markers / Diversidade genética em variedades de trigo duro argelino (Triticum turgidum L var. durum) usando marcadores microssatélites
Fonte: Biosci. j. (Online);34(6):1575-1583, nov./dec. 2018. tab, graf, ilus.
Idioma: en.
Resumo: Characterization of germplasm by DNA-markers provides powerful tool to precise germplasm identification. This study aimed to quantify the genetic diversity and to estimate the phylogenetic relationship among genotypes in many crop species. The results of the present study realized between Nov and Dec 2016 in biotechnologie unit (ICARDA, Morocco) which aimed to characterize a subset of 14 Algerian selected durum wheat cultivars (Triticum turgidum L. var. durum), using 13 SSR (Single Sequence Repeat) indicated the presence of a total of 39 alleles. The genetic diversity at the 13 microsatellites loci varied from 0,142 for Xgwm337 to 0.735 for Xgwm213 with a mean of 0.444. Polymorphic information content (PIC) values ranged from 0.13 to 0.70 and the genetic distance among the cultivars from 0.15 to 0.77. Clustering analysis showed that the studied varieties were grouped according to their population of origin, suggesting a provenance effect in their ordination. In fact the most similar varieties were those introduced from CIMMYT-ICARDA breeding program, which may have common parents in their pedigree. Selections from local landraces were more similar to each other and dissimilar to CIMMYT-ICARDA material, showing an agro-ecological adaptation.

A caracterização de germoplasma por marcadores de DNA fornece uma ferramenta poderosa para a identificação precisa de germoplasma, quantificar a diversidade genética e estimar a relação filogenética entre genótipos em muitas espécies de culturas. Os resultados do presente estudo foram realizados entre novembro e dezembro de 2016 na unidade de biotecnologia (ICARDA, Marrocos) que objetivou caracterizar um subconjunto de 14 cultivares de trigo duro argelinos selecionados (Triticum turgidum L. var. durum), utilizando 13 SSR (Single Sequence Repeat ) indicou a presença de um total de 39 alelos. A diversidade genética nos 13 locos de microssatélites variou de 0,142 para Xgwm337 a 0,735 para Xgwm213 com uma média de 0,444. Os valores do conteúdo de informação polimórfica (PIC) variaram de 0,13 a 0,70 e a distância genética entre as cultivares de 0,15 a 0,77. A análise de agrupamento mostrou que as variedades estudadas foram agrupadas de acordo com sua população de origem, sugerindo um efeito de proveniência em sua ordenação. De fato, as variedades mais similares foram aquelas introduzidas no programa de criação CIMMYT-ICARDA, que podem ter pais comuns em seu pedigree. Seleções de variedades locais foram mais similares entre si e diferentes do material CIMMYT-ICARDA, mostrando uma adaptação agroecológica.
Descritores: Variação Genética
Triticum
Repetições de Microssatélites
Responsável: BR396.1 - Biblioteca Central


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Id: lil-536335
Autor: Calcagnotto, Daniela; DeSalle, Rob.
Título: Population genetic structuring in pacu (Piaractus mesopotamicus) across the Paraná-Paraguay basin: evidence from microsatellites
Fonte: Neotrop. ichthyol;7(4):607-616, 2009. mapas, tab.
Idioma: en.
Projeto: FAPESP.
Resumo: The Paraná-Paraguay basin encompasses central western Brazil, northeastern Paraguay, eastern Bolivia and northern Argentina. The Pantanal is a flooded plain with marked dry and rainy seasons that, due to its soil characteristics and low declivity, has a great water holding capacity supporting abundant fish fauna. Piaractus mesopotamicus, or pacu, endemic of the Paraná-Paraguay basin, is a migratory species economically important in fisheries and ecologically as a potential seed disperser. In this paper we employ eight microsatellite loci to assess the population structure of 120 pacu sampled inside and outside the Pantanal of Mato Grosso. Our main objective was to test the null hypothesis of panmixia and to verify if there was a different structuring pattern between the Pantanal were there were no physical barriers to fish movement and the heavily impounded Paraná and Paranapanema rivers. All loci had moderate to high levels of polymorphism, the number of alleles varied from three to 18. The average observed heterozygosity varied from 0.068 to 0.911. After the Bonferroni correction three loci remained significant for deviations from Hardy-Weinberg, and for those the frequency of null alleles was estimated. F ST and R ST pairwise comparisons detected low divergence among sampling sites, and differentiation was significant only between Paranapanema and Cuiabá and Paranapanema and Taquari. No correlation between genetic distance and the natural logarithm of the geographic distance was detected. Results indicate that for conservation purposes and for restoration programs small genetic differences detected in the Cuiabá and Paranapanema rivers should be taken in consideration.(AU)

A bacia Paraná-Paraguai compreende o oeste do Brasil, nordeste do Paraguai, leste da Bolívia e o norte da Argentina. O Pantanal do Mato Grosso é uma planície inundada com estações de chuva e seca bem definidas, as características do solo e baixa declividade favorecem a retenção de água proporcionando abrigo para uma abundante ictiofauna. O Piaractus mesopotamicus, ou pacu, endêmico da bacia do Paraná-Paraguai, é uma espécie migratória com importância econômica na pesca e ecológica como potencial dispersor de sementes. Neste estudo utilizamos oito loci de microssatélites para verificar a estrutura populacional de 120 pacus coletados dentro e fora do Pantanal do Mato Grosso. Nosso principal objetivo foi testar a hipótese de panmixia e verificar se haviam diferentes padrões de estruturação entre o Pantanal onde não existem barreiras físicas ao movimento migratório desses peixes em relação aos rios Paraná e Paranapanema com suas inúmeras barragens. Todos os loci apresentaram níveis de polimorfismo de moderado a alto e o número de alelos variou de três a 18. A heterozigosidade média observada variou de 0,068 a 0,911. Depois da correção usando o método de Bonferroni três loci permaneceram estatisticamente significantes para desvios de Hardy-Weinberg, para estes a frequência de alelos nulos foi calculada. Comparações par a par de F ST e R ST detectaram baixa divergência genética entre os locais de coleta e as diferenças foram significantes apenas entre amostras do Paranapanema e Cuiabá e Paranapanema e Taquari. Não foi detectada correlação entre a diversidade genética e o logaritmo natural da distância geográfica. Os resultados indicam que as pequenas diferenças genéticas encontradas nos rios Cuiabá e Paranapanema devem ser levadas em consideração quando se tratar de programas de conservação desta espécie.(AU)
Descritores: Caraciformes/genética
-Repetições de Microssatélites
Estruturas Genéticas
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-530305
Autor: Matsumoto, Cristianne Kayoko; Hilsdorf, Alexandre Wagner Silva.
Título: Microsatellite variation and population genetic structure of a neotropical endangered Bryconinae species Brycon insignis Steindachner, 1877: implications for its conservation and sustainable management
Fonte: Neotrop. ichthyol;7(3):395-402, Sept. 2009. mapas, tab.
Idioma: en.
Projeto: International Foundation for Science; . FAPESP.
Resumo: Piabanha (Brycon insignis) is a freshwater fish species from the drainages in Southeastern Brazil. During the 1950s, it was an important economic and food resource for local populations, but dramatic and continuous environmental degradation seriously jeopardized the B. insignis populations in the region. Microsatellite markers were used to assess the genetic structure of wild populations of B. insignis and compare the genetic variability and integrity of the wild populations with a captive population. Samples of DNA from 208 specimens from geographically isolated populations were analyzed. Population genetic structure was investigated using F ST, R ST estimates as well as AMOVA. All five loci used in this study were polymorphic with observed heterozygosity ranging from 0.77 (± 0.15) to 0.88 (± 0.07) in the wild population and 0.90 (± 0.09) in the captive population and the allelic richness average were 7.56 (± 0.27) and 5.80 (± 1.02), respectively. Overall genetic differences were significantly partitioned among populations (F ST = 0.072, p = 0.034). Evidence of a genetic bottleneck was found in some of the wild populations, but especially in the captive population. The results showed that genetic variability still can be found in B. insignis populations which are currently structured possibly due to anthropic actions. The implications of these findings for the management and conservation of B. insignis populations are discussed.(AU)

Piabanha (Brycon insignis) é uma espécie de peixe de água doce oriunda de drenagens da região sudeste do Brasil. Durante os anos de 1950, esta espécie foi um importante recurso econômico para populações locais. Contudo, a intensa e contínua degradação ambiental afetou seriamente as populações de B. insignis na região. Marcadores microssatélites foram usados para avaliar a estrutura genética de populações selvagens de B. insignis e comparar a variabilidade genética e integridade das populações selvagens com uma população de cativeiro. Amostras de DNA de 208 espécimes de populações geograficamente isoladas foram analisadas. A estrutura populacional foi investigada usando-se estimadores de F ST e R ST bem como AMOVA. Todos os loci usados neste estudo foram polimórficos com heterozigosidades observadas variando de 0.77 (± 0.15) a 0.88 (± 0.77) em populações selvagens e 0.90 (± 0.09) na população de cativeiro e a riqueza alélica média foi de 7.56 (± 0.27) e 5.80 (± 1.02), respectivamente. A maioria das diferenças genéticas foi significativa entre populações (F ST = 0.072, p = 0.034). Evidências de efeito gargalo foram observadas em algumas populações selvagens e especialmente também na população de cativeiro. Os resultados do presente estudo mostraram que as populações de B. insignis ainda apresentam variabilidade genética e que estas populações estão atualmente estruturadas geneticamente provavelmente devido a ações antrópicas. As implicações destes achados para o manejo e conservação das populações de B. insiginis são discutidas.(AU)
Descritores: Repetições de Microssatélites/genética
Characidae/genética
-Estruturas Genéticas
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1021453
Autor: Chen, Caijin; He, Wenchuang; Yacouba Nassirou, Tondi; Nsabiyumva, Athanase; Dong, Xilong; Nevame Adedze, Yawo Mawunyo; Jin, Deming.
Título: Molecular characterization and genetic diversity of different genotypes of Oryza sativa and Oryza glaberrima
Fonte: Electron. j. biotechnol;30:48-57, nov. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Fundamental Research Funds for the Central Universities; . Specialized Research Fund for the Doctoral Program of Higher Education.
Resumo: Background: Availability of related rice species is critical for rice breeding and improvement. Two distinct species of domesticated rice exist in the genus Oryza: Oryza sativa (Asian rice) and Oryza glaberrima (African rice). New rice for Africa (NERICA) is derived from interspecific crosses between these two species. Molecular profiling of these germplasms is important for both genetics and breeding studies. We used 30 polymorphic SSR markers to assess the genetic diversity and molecular fingerprints of 53 rice genotypes of O. sativa, O. glaberrima, and NERICA. Results: In total, 180 alleles were detected. Average polymorphism information content and Shannon's information index were 0.638 and 1.390, respectively. Population structure and neighbor-joining phylogenetic tree revealed that 53 genotypes grouped into three distinct subpopulations conforming to the original three groups, except three varieties (IR66417, WAB450-4, MZCD74), and that NERICA showed a smaller genetic distance from O. sativa genotypes (0.774) than from O. glaberrima genotypes (0.889). A molecular fingerprint map of the 53 accessions was constructed with a novel encoding method based on the SSR polymorphic alleles. Ten specific SSR markers displayed different allelic profiles between the O. glaberrima and O. sativa genotypes. Conclusions: Genetic diversity studies revealed that 50 rice types were clustered into different subpopulations whereas three genotypes were admixtures. Molecular fingerprinting and 10 specific markers were obtained to identify the 53 rice genotypes. These results can facilitate the potential utilization of sibling species in rice breeding and molecular classification of O. sativa and O. glaberrima germplasms.
Descritores: Oryza/genética
Variação Genética
-Polimorfismo Genético
Cruzamento
Impressões Digitais de DNA
Repetições de Microssatélites
Genótipo
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1021043
Autor: Mei, Zhiqiang; Zhang, Xianqin; Khan, Md. Asaduzzaman; Imani, Saber; Liu, Xiaoyan; Zou, Hui; Wei, Chunli; Fu, Junjiang.
Título: Genetic analysis of Penthorum chinense Pursh by improved RAPD and ISSR in China
Fonte: Electron. j. biotechnol;30:6-11, nov. 2017. ilus, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Research Foundation of the Science and Technology Department of Sichuan Province Joint Program of the Science & Technology Office; . of Luzhou and the Science & Technology Department of Sichuan.
Resumo: Background: Penthorum chinense Pursh (P. chinense) is a well-known traditional Chinese medicine (TCM) plant, which has long been used for the prevention and treatment of hepatic diseases. This study aimed to genetically characterize the varieties of P. chinense from different geographic localities of China by random amplification of polymorphic DNA (RAPD)-PCR technique and verified with inter-simple sequence repeat (ISSR) markers. Results: The P. chinense samples were collected from nine different geographic localities. Previously improved RAPD and ISSR markers were utilized for genetic analysis using DNA amplification. The genetic relationship dendrogram was obtained by conducting cluster analysis to the similarity coefficient of improved RAPD and ISSR markers. Improved RAPD yielded 185 scorable amplified products, of which 68.6% of the bands were polymorphic, with an average amplification of 9.25 bands per primer. The ISSR markers revealed 156 alleles with 7.8 bands per primers, where 59.7% bands were polymorphic. Furthermore, the similarity coefficient ranges of RAPD and ISSR markers were 0.71­0.91 and 0.66­0.89, respectively. Conclusions: This study indicated that improved RAPD and ISSR methods are useful tools for evaluating the genetic diversity and characterizing P. chinense. Our findings can provide the theoretical basis for cultivar identification, standardization, and molecular-assisted breeding of P. chinense for medicinal use.
Descritores: Plantas Medicinais/genética
Magnoliopsida/genética
-Polimorfismo Genético
Variação Genética
Marcadores Genéticos
China
DNA de Plantas/genética
Técnica de Amplificação ao Acaso de DNA Polimórfico
Repetições de Microssatélites
Medicina Tradicional Chinesa
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1017082
Autor: Mei, Lanju; Dong, Na; Li, Fosheng; Li, Na; Yao, Min; Chen, Fang; Tang, Lin.
Título: Transcriptome analysis of female and male flower buds of Idesia polycarpa Maxim. var. vestita Diels
Fonte: Electron. j. biotechnol;29:39-46, sept. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Science and Technology support projects of Sichuan Province.
Resumo: Background: Idesia polycarpa Maxim. var. vestita Diels, a dioecious plant, is widely used for biodiesel due to the high oil content of its fruits. However, it is hard to distinguish its sex in the seedling stage, which makes breeding and production problematic as only the female tree can produce fruits, and the mechanisms underlying sex determination and differentiation remain unknown due to the lack of available genomic and transcriptomic information. To begin addressing this issue, we performed the transcriptome analysis of its female and male flower. Results: 28,668,977 and 22,227,992 clean reads were obtained from the female and male cDNA libraries, respectively. After quality checks and de novo assembly, a total of 84,213 unigenes with an average length of 1179 bp were generated and 65,972 unigenes (78.34%) could be matched in at least one of the NR, NT, Swiss-Prot, COG, KEGG and GO databases. Functional annotation of the unigenes uncovered diverse biological functions and processes, including reproduction and developmental process, which may play roles in sex determination and differentiation. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed many unigenes annotated as metabolic pathways, biosynthesis of secondary metabolites pathways, plant­ pathogen interaction, and plant hormone signal transduction. Moreover, 29,953 simple sequence repeats were identified using the microsatellite software. Conclusion: This work provides the first detailed transcriptome analysis of female and male flower of I. polycarpa and lays foundations for future studies on the molecular mechanisms underlying flower bud development of I. polycarpa.
Descritores: Reprodução/genética
Salicaceae/genética
Transcriptoma
-Análise de Sequência de RNA
Genes de Plantas
Repetições de Microssatélites
Salicaceae/crescimento & desenvolvimento
Bases de Dados Genéticas
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1015852
Autor: Yan, Kuan; Wei, Qin; Feng, Ruizhang; Zhou, Wanhai; Chen, Fang.
Título: Transcriptome analysis of Cinnamomum longepaniculatum by high-throughput sequencing
Fonte: Electron. j. biotechnol;28:58-66, July. 2017. tab, graf, ilus.
Idioma: en.
Projeto: Sichuan Education Department; . Innovative Program of Sichuan Undergraduates; . Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education.
Resumo: Background: Cinnamomum longepaniculatum is an important commercial crop and the main source of volatile terpenoids. The biosynthesis of key bioactive metabolites of C. longepaniculatum is not well understood because of the lack of available genomic and transcriptomic information. To address this issue, we performed transcriptome sequencing of C. longepaniculatum leaves to identify factors involved in terpenoid metabolite biosynthesis. Results: Transcriptome sequencing of C. longepaniculatum leaves generated over 56 million raw reads. The transcriptome was assembled using the Trinity software and yielded 82,061 unigenes with an average length of 879.43 bp and N50 value of 1387 bp. Furthermore, Benchmarking Universal Single-Copy Orthologs analysis indicated that our assembly is 91% complete. The unigenes were used to query the nonredundant database depending on sequence similarity; 42,809 unigenes were homologous to known genes in different species, with an annotation rate of 42.87%. The transcript abundance and Gene Ontology analyses revealed that numerous unigenes were associated with metabolism, while others were annotated in functional categories including transcription, signal transduction, and secondary metabolism. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 19,260 unigenes were involved in 385 metabolic pathways, with 233 unigenes found to be involved in terpenoid metabolism. Moreover, 23,463 simple sequence repeats were identified using the microsatellite identification tool. Conclusion: This is the first detailed transcriptome analysis of C. longepaniculatum. The findings provide insights into the molecular basis of terpenoid biosynthesis and a reference for future studies on the genetics and breeding of C. longepaniculatum.
Descritores: Terpenos/metabolismo
Cinnamomum/genética
Sequenciamento de Nucleotídeos em Larga Escala
Transcriptoma
-Transcrição Genética
Cruzamento
Óleos Voláteis/metabolismo
Repetições de Microssatélites
Anotação de Sequência Molecular
Ontologia Genética
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1009451
Autor: Silva, Geice Ribeiro da; Pereira, Fábia de Mello; Souza, Bruno de Almeida; Lopes, Maria Teresa do Rego; Campelo, José Elivalto Guimarães; Diniz, Fábio Mendonça.
Título: Aspectos bioecológicos e genético-comportamentais envolvidos na conservação da abelha Jandaíra, Melipona subnitida Ducke (Apidae, Meliponini), e o uso de ferramentas moleculares nos estudos de diversidade / Bioecological and behavioral genetic aspects involved in the conservation of the stingless bee Melipona subnitida Ducke (Apidae, Meliponini) and the use of molecular tools in studies of diversity
Fonte: Arq. Inst. Biol;81(3):299-308, July-Sept. 2014. ilus.
Idioma: pt.
Projeto: Embrapa.
Resumo: A abelha sem ferrão Melipona subnitida atualmente está presente em quase toda a região nordeste, em função da boa adaptabilidade ao semiárido nordestino e do potencial econômico-ecológico proporcionado pela produção de mel e pela polinização de cultivos em condições de confinamento. Apesar disso, é uma espécie ameaçada devido a processos de degradação ambiental, dentre os quais estão o desmatamento, o uso indiscriminado de agrotóxicos e o extrativismo. Tais interferências tendem a isolar as populações de Jandaíra, provocando uma queda na variabilidade genética e, consequentemente, uma redução na capacidade adaptativa da espécie. Porém, técnicas de biologia molecular estão sendo implementadas, possibilitando que populações desse tipo sejam avaliadas quanto ao seu grau de variabilidade genética. Marcadores moleculares do tipo microssatélites de DNA vêm sendo bastante usados, porém, em função do alto custo exigido para seu desenvolvimento, diversos estudos vêm empregando microssatélites transferidos de táxons próximos com amplo sucesso em estudos voltados à caracterização e à diversidade genética. Dessa forma, a presente revisão objetivou avaliar os mais relevantes aspectos bioecológicos e genético-comportamentais envolvidos na conservação da abelha Jandaíra, a fim de auxiliar na avaliação do grau de diversidade genética da espécie, bem como da sua distribuição entre indivíduos e populações da abelha sem ferrão M. subnitida.(AU)

The stingless bee Melipona subnitida is now present almost everywhere in the Brazilian Northeastern, as a consequence of its good adaptability to the semiarid and economic and ecological potential offered by the honey production and pollination of commercial crops under confined conditions. Nevertheless, it is an endangered species due to environmental degradation processes, among which are: deforestation, indiscriminate use of pesticides and honey extraction. Such interference tends to isolate populations of Jandaíra causing a decrease in genetic variability, and therefore a reduction in the adaptive capacity of the species. However, advanced Molecular Biology techniques have been used allowing such populations to be assessed for their degree of genetic variability. Molecular markers such as microsatellite DNA are widely applied to genetic diversity studies. However, due to the high costs required for their development, several studies have been focused on the use of microsatellites transferred from closely related taxa with much success in studies on the genetic characterization of species and their populations. Therefore, this review aimed to evaluate the most relevant ecological and behavioral aspects in order to assist the population genetic studies of the stingless bee M. subnitida.(AU)
Descritores: Variação Genética
Abelhas
-Repetições de Microssatélites
Tipo de Publ: Revisão
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: biblio-1009005
Autor: Shen, Zhan; Zhang, Kaiquan; Ma, Luyi; Duan, Jie; Ao, Yan.
Título: Analysis of the genetic relationships and diversity among 11 populations of Xanthoceras sorbifolia using phenotypic and microsatellite marker data
Fonte: Electron. j. biotechnol;26:33-39, Mar. 2017. ilus, tab, graf.
Idioma: en.
Projeto: International S&T Cooperation Program of China.
Resumo: Background: Assessments of genetic diversity are essential for germplasm characterization and exploitation. Molecular markers are valuable tools for exploring genetic variation and identifying germplasm. They play key roles in a Xanthoceras sorbifolia breeding program. Results: We analyzed the genetic diversity of populations of this species using 23 simple sequence repeat (SSR) loci and data on kernel oil content. The 11 populations included in the study were distributed across a large geographic range in China. The kernel oil content differed significantly among populations. The SSR marker analysis detected high genetic diversity among the populations. All SSRs were polymorphic, and we identified 80 alleles across the populations. The number of alleles at each locus ranged from two to six, averaging 3.48 per primer pair. The polymorphism information content values ranged from 0.35 to 0.70, averaging 0.51. Expected heterozygosity, observed heterozygosity, and Shannon's information index calculations detected large genetic variations among populations of different provenance. The high average number of alleles per locus and the allelic diversity observed in the set of genotypes analyzed indicated that the genetic base of this species was relatively wide. The statistically significant positive correlation between genetic and geographic distances suggested adaptations to local conditions. Conclusions: Microsatellite markers can be used to efficiently distinguish X. sorbifolia populations and assess their genetic diversity. The information we have provided will contribute to the conservation and management of this important plant genetic resource.
Descritores: Variação Genética
Repetições de Microssatélites
Sapindaceae/genética
-Fenótipo
Polimorfismo Genético
Sementes/genética
Óleos Vegetais
Marcadores Genéticos
China
Reação em Cadeia da Polimerase
DNA de Plantas
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1008710
Autor: Lei, Chao; Bei, Weilie; Wang, Xueying; Du, Xiaodong; Jiao, Yu; Huang, Ronglian; Wang, Qingheng; Deng, Yuewen.
Título: Development of SSR marker by RNA-seq and its application in genotyping pearl sac in pearl oyster Pinctada fucata martensii
Fonte: Electron. j. biotechnol;25:70-74, ene. 2017. tab, graf, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Administration of Ocean and Fisheries of Guangdong Province.
Resumo: Background: Pearl oyster Pinctada fucata martensii is cultured for producing round nucleated pearls. Pearl production involves a surgical operation where a mantle tissue graft from a donor oyster and a round nucleus are implanted in the gonad of a host oyster. Whether the mantle graft implanted in the gonad of a host oyster contributes to the formation of a pearl sac that secretes pearl nacre to form a pearl should be determined. In April 2012, two full-sib families were separately used as donor and host oysters for a nucleus insertion operation. The pearl sac was sampled from the host oysters at day 60 after nucleus operation. A large number of simple sequence repeat (SSR) markers were developed using Illumina HiSeq™ 2000 platform. The two full-sib families were also used to mine diagnostic SSR markers for genotyping donor oyster, host oyster, and pearl sac. Results: A total of 3168 microsatellite loci were identified in 39,078 unigenes, and 1977 SSR primers were designed by Primer 3.0. Forty-seven SSR primers were validated, and the rate of successful amplification was 72.3%. Two diagnostic SSR primers could successfully genotype pearl sac, donor oyster, and host oyster. Donor and host oysters were both homogenous, and the alleles in pearl sac were identical to those in donor and host oysters. Conclusions: The present results confirmed that the mantle graft implanted in the gonad of host oyster contributed to the formation of the pearl sac in pearl oyster P. fucata martensii.
Descritores: Transplante
Repetições de Microssatélites/genética
Pinctada/genética
-Reação em Cadeia da Polimerase
Técnicas de Genotipagem
Limites: Animais
Responsável: CL1.1 - Biblioteca Central



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