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Id: biblio-1026852
Autor: Gutiérrez-Velázquez, Marcela Verónica; Almaraz-Abarca, Norma; Herrera-Arrieta, Yolanda; Ávila-Reyes, José Antonio; González-Valdez, Laura Silvia; Torres-Ricario, Rene; Uribe-Soto, José Natividad; Monreal-García, Hugo Manuel.
Título: Comparison of the phenolic contents and epigenetic and genetic variability of wild and cultivated watercress (Rorippa nasturtium var. aquaticum L.)
Fonte: Electron. j. biotechnol;34:9-16, july. 2018. tab, graf.
Idioma: en.
Projeto: Consejo Nacional de Ciencia y Tecnología.
Resumo: Background: Epigenetic modifications are key factors modulating the expression of genes involved in the synthesis of phytochemicals. The knowledge of plant epigenetic and genetic variations can contribute to enhance the production of bioactive compounds. These issues have been little explored thus far in Rorippa nasturtium var. aquaticum L. (watercress), an edible and medicinal plant. The aim of the current study was to determine and compare the phenolic composition and epigenetic and genetic variations between wild and cultivated watercress. Results: Significant differences were found in the quantitative phenolic composition between wild and cultivated watercress. The eight primer combinations used in the methylation-sensitive amplification polymorphism (MSAP) method revealed different epigenetic status for each watercress type, the cultivated one being the most epigenetically variable. The genetic variability revealed by the EcoRI/MspI amplification profile and also by eight inter-simple sequence repeat (ISSR) primers was different between the two types of watercress. The results of the Mantel test showed that the correlation between genetic and epigenetic variations has diminished in the cultivated type. Cluster analyses showed that the epigenetic and genetic characterizations clearly discriminated between wild and cultivated watercress. Conclusions: Relevant chemical, epigenetic, and genetic differences have emerged between wild and cultivated watercress. These differences can contribute to fingerprint and develop quality control tools for the integral and safety use and the commercialization of watercress. The richness of epialleles could support the development of tools to manipulate the watercress epigenome to develop high bioproduct­producing cultivars
Descritores: Nasturtium/genética
Nasturtium/química
-Plantas Comestíveis
Variação Genética
Análise por Conglomerados
Repetições de Microssatélites
Metilação de DNA
Brassicaceae/genética
Brassicaceae/química
Citosina/metabolismo
Compostos Fenólicos/análise
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Epigenômica
Compostos Fitoquímicos
Responsável: CL1.1 - Biblioteca Central


  2 / 290 LILACS  
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Id: biblio-1047452
Autor: Jiang, Liwen; Nie, Hongtao; Li, Chen; Li, Dongdong; Huo, Zhongming; Yan, Xiwu.
Título: The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
Fonte: Electron. j. biotechnol;34:17-21, july. 2018. tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Modern Agro-industry Technology Research System; . Program for Liaoning Excellent Talents in University; . Cultivation Plan for Youth Agricultural Science and Technology Innovative Talents of Liaoning Province; . Natural Science Foundation of Dalian; . Dalian Youth Science and Technology Star Project Support Program; . Scientific Research project of Liaoning Education Department.
Resumo: Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via highthroughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy­Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.
Descritores: Variação Genética
Bivalves/genética
Repetições de Microssatélites
-Polimorfismo Genético
Aquicultura
Loci Gênicos
Genética Populacional
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  3 / 290 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-1287437
Autor: García-Castro, Kevin León; Rangel-Medrano, José David; Landínez-García, Ricardo Marcel; Márquez, Edna Judith.
Título: Population genetics of the endangered catfish Pseudoplatystoma magdaleniatum (Siluriformes: Pimelodidae) based on species-specific microsatellite loci
Fonte: Neotrop. ichthyol;19(1):e200120, 2021. tab, graf.
Idioma: en.
Projeto: Universidad Nacional de Colombia Sede Medellín and Empresas Públicas de Medellín.
Resumo: The Neotropical catfish genus Pseudoplatystoma comprises eight species of large size, widely distributed in South American basins. The endangered species P. magdaleniatum is endemic to Magdalena basin (Colombia), experiences high fishing pressure and its population genetics is relatively unknown. To study the genetic status and structure of P. magdaleniatum, 25 species-specific polymorphic microsatellite loci were developed using next-generation sequencing and then tested in samples collected in the Magdalena-Cauca basin. Based on 15 of these loci, P. magdaleniatum showed a high number of alleles per locus (9-10), high values of observed (0.762-0.798) and expected (0.770-0.791) heterozygosities, recent reduction of population size and gene flow. These findings constitute a baseline to measure potential changes in genetic diversity and structure of this commercially important species in a basin undergoing high anthropogenic activities.(AU)

El género de bagres neotropicales Pseudoplatystoma comprende ocho especies de gran tamaño, ampliamente distribuidas en las cuencas de Suramérica. La especie en peligro de extinción P. magdaleniatum es endémica de la Cuenca del Magdalena (Colombia), experimenta una alta presión pesquera y su genética poblacional es relativamente desconocida. Para estudiar el estado y estructura genética de P. magdaleniatum, se desarrollaron 25 loci microsatélites polimórficos especie específicos utilizando secuenciación de próxima generación y se evaluaron en muestras recolectadas en la Cuenca del Magdalena-Cauca. Con base en 15 loci, P. magdaleniatum mostró un alto número de alelos por locus (9-10), valores altos de heterocigosidad observada (0.762-0.798) y esperada (0.770-0.791), reducción reciente del tamaño poblacional y flujo génico. Estos hallazgos constituyen una línea de base para medir cambios potenciales en la diversidad y estructura genética de esta especie comercialmente importante en una cuenca sometida a altas actividades antropogénicas.(AU)
Descritores: Variação Genética
Pesos e Medidas
Peixes-Gato
Repetições de Microssatélites
-Espécies em Perigo de Extinção
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  4 / 290 LILACS  
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Id: biblio-1253080
Autor: Liu, Fei; Qu, Yun-Kun; Geng, Chao; Wang, Ai-Ming; Zhang, Jia-Hong; Li, Jin-Feng; Chen e, Kai-Jian; Liu, Bo; Tian, Hong-Yan; Yang, Wen-Ping; Yu, Ye-Bing.
Título: Analysis of the population structure and genetic diversity of the red swamp crayfish (Procambarus clarkii) in China using SSR markers
Fonte: Electron. j. biotechnol;47:59-71, sept. 2020. tab, ilus, graf.
Idioma: en.
Projeto: Jiangsu Province Agricultural Science and Technology Independent Innovation Fund Project; . Jiangsu Agricultural Industry Technology System (Red Swamp Crayfish; . China Agriculture Research System; . Yancheng Institute of Technology Talent Introduction Project; . Jiangsu Science and Technology Project - Northern; . Jiangsu Science and Technology Special Project.
Resumo: BACKGROUND: Procambarus clarkii produces high-quality, delicious meat that is high in protein, low in fat, and rich in calcium and phosphorus. It has become an important aquatic resource in China. Our objectives are (i) to analyze the level of genetic diversity of P. clarkii populations; (ii) to explore the genetic differentiation (Gst); and (iii) to propose appropriate strategies for the conservation. RESULTS: In this study, Shannon's index (I) and Nei's gene diversity index (H) for P. clarkii were high (I = 0.3462 and H = 0.2325 on average and I = 0.6264, H = 0.4377 at the species level) based on the SSR markers. The expected heterozygosity value of 17 microsatellite loci in 25 crayfish populations was 0.9317, the observed heterozygosity value was 0.9121, and the observed number of alleles per locus was 2.000; and the effective number of alleles per locus was 1.8075. Among the P. clarkii populations, the inbreeding coefficient within populations (Fis) was 0.2315, overall inbreeding coefficient (Fit) was 0.4438, genetic differentiation coefficient among populations (Fst) was 0.3145 and gene differentiation (Gst) was 0.4785 based on SSR analyses. The cluster analysis results obtained by unweighted pair-group method with arithmetic mean (UPGMA) analysis, principal coordinate analysis (PCoA) and STRUCTURE analysis were similar. A mantel test showed that the isolation-by-distance pattern was not significant. CONCLUSIONS: The high Gst among P. clarkii populations is attributed to genetic drift and geographic isolation. The results indicated that more P. clarkii populations should be collected when formulating conservation and aquaculture strategies.
Descritores: Variação Genética
Repetições de Microssatélites
Astacoidea/genética
-Filogenia
China
Reação em Cadeia da Polimerase
Aquicultura
Ambiente Aquático
Áreas Alagadas
Triagem de Portadores Genéticos
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  5 / 290 LILACS  
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Id: biblio-1049063
Autor: Wisniewska, Halina; Majka, Maciej; Kwiatek, Michal; Gawlowska, Magdalena; Surma, Maria; Adamski, Tadeusz; Kaczmarek, Zygmunt; Drzazga, Tadeusz; Lugowska, Boguslawa; Korbas, Marek; Belter, Jolanta.
Título: Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection
Fonte: Electron. j. biotechnol;37:11-17, Jan. 2019. tab, ilus.
Idioma: en.
Projeto: National Centre for Research and Development.
Resumo: BACKGROUND: Wheat is one of the most important crops cultivated all over the world. New high-yielding cultivars that are more resistant to fungal diseases have been permanently developed. The present study aimed at the possibility of accelerating the process of breeding new cultivars, resistant to eyespot, by using doubled haploids (DH) system supported by marker-assisted selection. RESULTS: Two highly resistant breeding lines (KBP 0916 and KBH 4942/05) carrying Pch1 gene were crossed with the elite wheat genotypes. Hybrid plants of early generations were analyzed using endopeptidase EpD1 and two SSR markers linked to the Pch1 locus. Selected homozygous and heterozygous genotypes for the Pch1-linked EpD1b allele were used to produce haploid plants. Molecular analyses were performed on haploids to identify plants possessing Pch1 gene. Chromosome doubling was performed only on haploid plants with Pch1 gene. Finally, 65 DH lines carrying eyespot resistance gene Pch1 and 30 lines without this gene were chosen for the eyespot resistance phenotyping in a field experiment. CONCLUSIONS: Results of the experiment confirmed higher resistance to eyespot of the genotypes with Pch1 in comparison to those without this gene. This indicates the efficiency of selection at the haploid level.
Descritores: Seleção Genética
Triticum/genética
Triticum/metabolismo
Haploidia
-Doenças das Plantas
Cruzamento/métodos
Expressão Gênica
Repetições de Microssatélites
Genótipo
Responsável: CL1.1 - Biblioteca Central


  6 / 290 LILACS  
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Id: biblio-1254683
Autor: Xu, Qiaoyue; Zheng, Junhong; Yan, Xiwu; Nie, Hongtao.
Título: Genetic diversity and differentiation of nine populations of the hard clam (Meretrix petechialis) assessed by EST-derived microsatellites
Fonte: Electron. j. biotechnol;48:23-28, nov. 2020. tab, ilus.
Idioma: en.
Projeto: Chinese Ministry of Science and Technology through the National Key Research and Development Programme of China; . Modern Agro-industry Technology Research System; . Dalian Youth Science and Technology Star Project Support Program.
Resumo: BACKGROUND: Meretrix petechialis is one of the commercially important marine bivalves. In this study, we selected six highly polymorphic EST-derived microsatellite markers to assess the genetic diversity and population differentiation on nine wild populations of Meretrix petechialis. RESULTS: The number of alleles detected per-locus ranged from 4 to 30 (mean NA = 27.5) with a total of 165 alleles. The mean value of observed and expected heterozygosities varied from 0.717 to 0.861 and from 0.797 to 0.856, respectively. Meanwhile, the result of Neighbor-joining and overall FST = 0.214 (P < 0.01) reveled that M. petechialis populations from GX are the farthest populations, a certain degree of genetic variation among individuals in each population and the genetic differentiation is significant. CONCLUSIONS: GX population has high genetic diversity among individual, and there are certain differences in genetic characteristics among different populations. This study will provide a basis for the domestication and cultivation of genetic diversity of M. petechialis population and the protection of clam germplasm resources.
Descritores: Variação Genética
Bivalves/genética
Repetições de Microssatélites
Etiquetas de Sequências Expressas
-População
Alelos
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  7 / 290 LILACS  
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Id: biblio-1223243
Autor: Aziz, Sadia; Firdous, Syeda; Rahman, Hifz; Iqbal Awan, Shahid; Michael, Vincent; Meru, Geoffrey.
Título: Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan
Fonte: Electron. j. biotechnol;46:50-54, jul. 2020. tab, graf, ilus.
Idioma: en.
Resumo: BACKGROUND: Pomegranate (Punica granatum L.), one of the most important tropical fruits in Azad Jammu and Kashmir regions of Pakistan, is highly valued for its nutrition and medicinal purposes. Although pomegranate is native to this region, the genetic diversity among wild pomegranate accessions is currently unknown. Such information would be vital for germplasm conservation and breeding efforts. In the current study, genetic diversity among forty-eight wild pomegranate accessions collected from different agro-ecological zones of Azad Jammu and Kashmir was assessed using 41 simple sequence repeat (SSR) markers. RESULTS: The markers revealed 303 alleles averaging 7.39 alleles per marker. Polymorphic information content ranged from 0.12 (PGCT093B) to 0.88 (Pom006), with a mean of 0.54. The average genetic distance (GD) across all genotypes was 0.52, and was lowest between Chattar Class and Thorar genotypes (GD = 0.27), but highest between Khun Bandway and Akhor Ban (GD = 0.74). A neighbor-joining dendrogram separated the genotypes into three major clusters, with further sub-clustering within each cluster. CONCLUSIONS: Overall, the results presented here show significant genetic diversity among wild pomegranate accessions in Azad Jammu and Kashmir region of Pakistan. These accessions present a valuable genetic resource to breeding and cultivar improvement programs within the region.
Descritores: Variação Genética
Romã (Fruta)/genética
-Paquistão
DNA
Repetições de Microssatélites
Alelos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1142426
Autor: Mogollón, Fernanda; Casas Vargas, Andrea; Rodríguez, Fredy; Usaquén, William.
Título: Twins from different fathers: A heteropaternal superfecundation case report in Colombia / Gemelos de diferentes padres: un caso de superfecundación heteropaternal en Colombia
Fonte: Biomédica (Bogotá);40(4):604-608, oct.-dic. 2020. tab.
Idioma: en.
Resumo: Abstract: Heteropaternal superfecundation is an extremely rare phenomenon that occurs when a second ova released during the same menstrual cycle is additionally fertilized by the sperm cells of a different man in separate sexual intercourse. In August, 2018, the Grupo de Genética de Poblaciones e Identificación at Universidad Nacional de Colombia received a request to establish the paternity of a pair of male twins with genetic markers. The following analyses were performed: amelogenin gene, autosomal short tandem repeat (STR), and Y-STR analyses by means of human identification commercial kits, paternity index, and the probability of paternity calculation and interpretation. A paternity index of 2.5134E+7 and a probability of paternity of 99.9999% for twin 2 were obtained while 14 out of 17 Y-chromosome markers and 14 out of 21 autosomal short tandem repeats were excluded for twin 1. The results indicated that the twins have different biological fathers. Although heteropaternal superfecundation is rarely observed among humans given its low frequency, in paternity disputes for dizygotic twins it is mandatory to demand the presence of the two twins in the testing to avoid wrong conclusions.

Resumen: La superfecundación heteropaternal es un fenómeno extremadamente raro que se produce cuando un segundo óvulo, liberado durante el mismo ciclo menstrual, es fertilizado por un espermatozoide de un hombre diferente en relaciones sexuales separadas. En agosto de 2018, el Grupo de Genética de Poblaciones e Identificación de la Universidad Nacional de Colombia recibió una solicitud para establecer la paternidad mediante marcadores genéticos de un par de mellizos varones, en quienes se hizo el análisis del gen de amelogenina, el análisis de repeticiones cortas en tándem (Short Tandem Repeats, STR) autosómicas y del cromosoma Y (Y-STR) mediante kits comerciales de identificación humana y cálculos e interpretación del índice de paternidad y probabilidad de paternidad. Se obtuvo un índice de paternidad de 2,5134E+7 y una probabilidad de paternidad de 99,9999 % para el gemelo 2, en tanto que en el gemelo 1 se excluyeron 14 de los 17 marcadores del cromosoma Y y 14 de los 21 sistemas STR autosómicos evaluados. Los resultados indicaron que los gemelos tienen diferentes padres biológicos. A pesar de que la superfecundación heteropaternal rara vez se observa en humanos debido a su baja frecuencia, en las disputas de paternidad para los gemelos dicigóticos, es obligatorio exigir en la prueba la presencia de los dos gemelos para evitar conclusiones incorrectas.
Descritores: Gêmeos Dizigóticos
-Paternidade
Impressões Digitais de DNA
Repetições de Microssatélites
Fertilização
Tipo de Publ: Relatos de Casos
Responsável: CO42.1 - Biblioteca Nacional de Salud José Celestino Mutis


  9 / 290 LILACS  
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Texto completo SciELO Chile
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Id: biblio-1124215
Autor: Fernandes-Rocha, Lucas; Ribeiro-Paula, Natália; Gonçalves-Nazareno, Alison; Carvalho, Dulcinéia de.
Título: Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
Fonte: Biol. Res;53:30, 2020. tab.
Idioma: en.
Projeto: FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais); . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES).
Resumo: BACKGROUND: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. RESULTS: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed ( HO ) and expected ( HE ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. CONCLUSIONS: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.
Descritores: Espécies em Perigo de Extinção
Repetições de Microssatélites/genética
Asteraceae/genética
-Brasil
Alelos
Responsável: CL1.1 - Biblioteca Central


  10 / 290 LILACS  
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Texto completo SciELO Chile
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Id: biblio-1124206
Autor: Li, Bin; Lin, Furong; Huang, Ping; Guo, Wenying; Zheng, Yongqi.
Título: Development of nuclear SSR and chloroplast genome markers in diverse Liriodendron chinense germplasm based on low-coverage whole genome sequencing
Fonte: Biol. Res;53:21, 2020. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Forest and Grass Germplasm Resources Bank Program.
Resumo: BACKGROUND: Liriodendron chinense ranges widely in subtropical China and northern Vietnam; however, it inhabits several small, isolated populations and is now an endangered species due to its limited seed production. The objective of this study was to develop a set of nuclear SSR (simple sequence repeats) and multiple chloroplast genome markers for genetic studies in L. chinense and their characterization in diverse germplasm. RESULTS: We performed low-coverage whole genome sequencing of the L. chinense from four genotypes, assembled the chloroplast genome and identified nuclear SSR loci by searching in contigs for SSR motifs. Comparative analysis of the four chloroplast genomes of L. chinense revealed 45 SNPs, 17 indels, 49 polymorphic SSR loci, and five small inversions. Most chloroplast intraspecific polymorphisms were located in the interspaces of single-copy regions. In total, 6147 SSR markers were isolated from low-coverage whole genome sequences. The most common SSR motifs were dinucleotide (70.09%), followed by trinucleotide motifs (23.10%). The motif AG/TC (33.51%) was the most abundant, followed by TC/AG (25.53%). A set of 13 SSR primer combinations were tested for amplification and their ability to detect polymorphisms in a set of 109 L. chinense individuals, representing distinct varieties or germplasm. The number of alleles per locus ranged from 8 to 28 with an average of 21 alleles. The expected heterozygosity (He) varied from 0.19 to 0.93 and the observed heterozygosity (Ho) ranged from 0.11 to 0.79. CONCLUSIONS: The genetic resources characterized and tested in this study provide a valuable tool to detect polymorphisms in L. chinense for future genetic studies and breeding programs.
Descritores: Polimorfismo Genético/genética
Genoma de Planta/genética
Liriodendron/genética
Genoma de Cloroplastos/genética
-Primers do DNA/genética
DNA de Plantas/genética
Repetições de Microssatélites
Alelos
Sequenciamento Completo do Genoma
Genótipo
Responsável: CL1.1 - Biblioteca Central



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