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Texto completo SciELO Brasil
AVILA-CAMPOS, Mario Julio
Texto completo
Id: lil-531394
Autor: Vieira, Evanice Menezes Marçal; Raslan, Suzane A; Wahasugui, Thais Cristina; Avila-Campos, Mario Julio; Marvulle, Valdecir; Gaetti-Jardim Júnior, Elerson.
Título: Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian indians from Umutina Reservation, Mato Grosso, Brazil
Fonte: J. appl. oral sci;17(5):440-445, Sept.-Oct. 2009. graf.
Idioma: en.
Projeto: Fundação do Amparo à Pesquisa do Estado de São Paulo.
Resumo: Aggregatibacter actinomycetemcomitans is associated with periodontal disease, especially localized aggressive periodontitis, produces a potent leukotoxin and its distribution is influenced by ethnic characteristics of the population. Objective: Using culture and polymerase chain reaction (PCR) techniques, this study evaluated the occurrence of this microorganism and the distribution of leukotoxic strains isolated from Indians belonging to the Umutima Reservation, Mato Grosso, Brazil. MATERIAL AND METHODS: Forty-eight native Brazilians with gingivitis and 38 with chronic periodontitis, belonging to Umutina, Paresi, Bororo, Bakairi, Kayabi, Irantxe, Nambikwara and Terena ethnicities, were studied. Subgingival, supragingival and saliva samples of each patient were collected and transferred to VMGA III medium and to ultra pure Milli Q water. Bacteria were grown on TSBV agar and incubated in anaerobiosis (90 percent N2 + 10 percent CO2) at 37ºC for 72 h. The presence of the ltx promoter was determined by PCR, and a 530 bp deletion in the promoter was evaluated by using specific primers. RESULTS: A. actinomycetemcomitans was isolated from 8.33 percent of saliva, supragingival and subgingival samples from patients with gingivitis and from 18.42 percent of saliva and supragingival biofilm, and 26.32 percent subgingival biofilm from patients with chronic periodontitis. By PCR, the bacterial DNA was detected in 8.33 percent of saliva, supragingival and subgingival biofilms from patients with gingivitis and from 23.68 percent of saliva, 28.95 percent supragingival biofilm and 34.21 percent subgingival biofilm from patients with periodontitis. All strains were grouped as non-JP2 clones based on the absence of deletion in the leukotoxin promoter. Differences among the microbial and clinical parameters in patients were analyzed by using the Mann-Whitney, Chi-square or Fisher's exact tests. CONCLUSIONS: The present results suggest that A. actinomycetemcomitans ...
Descritores: Aggregatibacter actinomycetemcomitans/isolamento & purificação
Periodontite Crônica/microbiologia
Gengivite/microbiologia
Índios Sul-Americanos
-Fatores Etários
Aggregatibacter actinomycetemcomitans/classificação
Biofilmes
Toxinas Bacterianas/análise
Pareamento de Bases/genética
Sequência de Bases/genética
Brasil/etnologia
Dispositivos para o Cuidado Bucal Domiciliar
Placa Dentária/microbiologia
Exotoxinas/análise
Gengiva/microbiologia
Hemorragia Gengival/microbiologia
Perda da Inserção Periodontal/microbiologia
Bolsa Periodontal/microbiologia
Regiões Promotoras Genéticas/genética
Saliva/microbiologia
Deleção de Sequência/genética
Escovação Dentária
Adulto Jovem
Limites: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Humanos
Pessoa de Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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