Base de dados : LILACS
Pesquisa : G02.111.570.820.709.275.500 [Categoria DeCS]
Referências encontradas : 12 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2 ir para página        

  1 / 12 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-751414
Autor: França, Monique Sedlmaier; Usón Junior, Pedro Luiz Serrano; Antunes, Yuri Philippe Pimentel Vieira; Prado, Bernard Lobato; Donnarumma, Carlos del Cistia; Mutão, Taciana Sousa; Rodrigues, Heloisa Veasey; Giglio, Auro del.
Título: Assessment of adherence to the guidelines for the management of nausea and vomiting induced by chemotherapy / Avaliação da aderência à diretriz de cuidados para náuseas e vômitos induzidos por quimioterapia
Fonte: Einstein (Säo Paulo);13(2):221-225, Apr-Jun/2015. tab.
Idioma: en.
Resumo: ABSTRACT Objective: To assess adherence of the prescribing physicians in a private cancer care center to the American Society of Clinical Oncology guideline for antiemetic prophylaxis, in the first cycle of antineoplastic chemotherapy. Methods: A total of 139 chemotherapy regimens, of 105 patients, were evaluated retrospectively from 2011 to 2013. Results: We observed 78% of non-adherence to the guideline rate. The main disagreements with the directive were the prescription of higher doses of dexamethasone and excessive use of 5-HT3 antagonist for low risk emetogenic chemotherapy regimens. On univariate analysis, hematological malignancies (p=0.005), the use of two or more chemotherapy (p=0.05) and high emetogenic risk regimes (p=0.012) were factors statistically associated with greater adherence to guidelines. Treatment based on paclitaxel was the only significant risk factor for non-adherence (p=0.02). By multivariate analysis, the chemotherapy of high emetogenic risk most correlated with adherence to guideline (p=0.05). Conclusion: We concluded that the adherence to guidelines is greater if the chemotherapy regime has high emetogenic risk. Educational efforts should focus more intensely on the management of chemotherapy regimens with low and moderate emetogenic potential. Perhaps the development of a computer generated reminder may improve the adherence to guidelines. .

RESUMO Objetivo: Avaliar a adesão dos médicos prescritores, de um centro privado especializado em oncologia, à diretriz de antiêmese profilática da American Society of Clinical Oncology, no primeiro ciclo de quimioterapia antineoplásica. Métodos: Foram avaliados retrospectivamente 139 esquemas de quimioterapia, de 105 pacientes, tratados no período de 2011 a 2013. Resultados: Foram observados 78% de taxa de não adesão à diretriz. As principais discordâncias com a diretriz foram prescrição de doses mais elevadas de dexametasona e uso excessivo de antagonista 5-HT3 para regimes de quimioterapia de risco emetogênico baixo. Pela análise univariada, malignidades hematológicas (p=0,005), uso de dois ou mais quimioterápicos (p=0,05) e regimes de alto risco emetogênico (p=0,012) foram fatores estatisticamente associados a maior adesão à diretriz. O tratamento baseado em paclitaxel foi o único fator estatisticamente significativo para a não adesão (p=0,02). Pela análise multivariada, a quimioterapia de alto risco emetogênico apresentou maior correlação com a adesão à diretriz (p=0,05). Conclusão: Houve maior aderência para a quimioterapia de alto risco emetogênico. Esforços educacionais devem se concentrar mais intensamente na gestão de regimes de quimioterapia com potencial emetogênico baixo e moderado. Talvez o desenvolvimento de lembretes gerados por sistemas informatizados possa melhorar a aderência à diretriz. .
Descritores: Dano ao DNA
Reparo de DNA por Recombinação
Ubiquitina-Proteína Ligases/química
-Motivos de Aminoácidos
Sequência de Aminoácidos
Proteína BRCA1/antagonistas & inibidores
Linhagem Celular
Quebra Cromossômica
Sequência Conservada
Reparo do DNA
Proteínas de Ligação a DNA/antagonistas & inibidores
Desoxirribonucleases/metabolismo
Histonas/metabolismo
Estrutura Terciária de Proteína
Ubiquitinação
Ubiquitina-Proteína Ligases/metabolismo
Limites: Animais
Seres Humanos
Camundongos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-751309
Autor: Pereira, Elisângela Vitoriano; Costa, Jorge de Assis; Alfenas, Rita de Cássia Gonçalves.
Título: Effect of glycemic index on obesity control
Fonte: Arch. endocrinol. metab. (Online);59(3):245-251, 06/2015. tab, graf.
Idioma: en.
Resumo: Objective Evaluate the effect of glycemic index (GI) on biochemical parameters, food intake, energy metabolism, anthropometric measures and body composition in overweight subjects.Materials and methods Simple blind study, in which nineteen subjects were randomly assigned to consume in the laboratory two daily low GI (n = 10) or high GI (n = 9) meals, for forty-five consecutive days. Habitual food intake was assessed at baseline. Food intake, anthropometric measures and body composition were assessed at each 15 days. Energy metabolism and biochemical parameters were evaluated at baseline and the end of the study.Results Low GI meals increased fat oxidation, and reduced waist circumference and HOMA-IR, while high GI meals increased daily dietary fiber and energy intake compared to baseline. There was a higher reduction on waist circumference and body fat, and a higher increase on postprandial fat oxidation in response to the LGI meals than after high GI meals. High GI meals increased fasting respiratory coefficient compared to baseline and low GI meals.Conclusion The results of the present study showed that the consumption of two daily low GI meals for forty-five consecutive days has a positive effect on obesity control, whereas, the consumption of high GI meals result has the opposite effect. Arch Endocrinol Metab. 2015;59(3):245-51.
Descritores: Proteínas de Bactérias/química
Escherichia coli/enzimologia
Proteínas de Membrana/química
Fenilalanina/química
-Motivos de Aminoácidos
Sequência de Aminoácidos
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Quimiotaxia
Sequência Conservada
Dimerização
Escherichia coli/química
Escherichia coli/genética
Escherichia coli/fisiologia
Dados de Sequência Molecular
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Conformação Proteica
Fenilalanina/genética
Fenilalanina/metabolismo
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME


  3 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-732608
Autor: Braga, Lucia Libanez Bessa Campelo; Oliveira, Maria Aparecida Alves de; Gonçalves, Maria Helane Rocha Batista; Chaves, Fernando Kennedy; Benigno, Tiago Gomes da Silva; Gomes, Adriana Dias; Silva, Cícero Igor Simões Moura; Anacleto, Charles; Batista, Sérgio de Assis; Queiroz, Dulciene Maria Magalhães.
Título: CagA phosphorylation EPIYA-C motifs and the vacA i genotype in Helicobacter pylori strains of asymptomatic children from a high-risk gastric cancer area in northeastern Brazil
Fonte: Mem. Inst. Oswaldo Cruz;109(8):1045-1049, 12/2014. tab.
Idioma: en.
Resumo: Helicobacter pylori infection is one of the most common infections worldwide and is associated with gastric diseases. Virulence factors such as VacA and CagA have been shown to increase the risk of these diseases. Studies have suggested a causal role of CagA EPIYA-C in gastric carcinogenesis and this factor has been shown to be geographically diverse. We investigated the number of CagA EPIYA motifs and the vacA i genotypes in H. pylori strains from asymptomatic children. We included samples from 40 infected children (18 females and 22 males), extracted DNA directly from the gastric mucus/juice (obtained using the string procedure) and analysed the DNA using polymerase chain reaction and DNA sequencing. The vacA i1 genotype was present in 30 (75%) samples, the i2 allele was present in nine (22.5%) samples and both alleles were present in one (2.5%) sample. The cagA-positive samples showed distinct patterns in the 3’ variable region of cagA and 18 of the 30 (60%) strains contained 1 EPIYA-C motif, whereas 12 (40%) strains contained two EPIYA-C motifs. We confirmed that the studied population was colonised early by the most virulent H. pylori strains, as demonstrated by the high frequency of the vacA i1 allele and the high number of EPIYA-C motifs. Therefore, asymptomatic children from an urban community in Fortaleza in northeastern Brazil are frequently colonised with the most virulent H. pylori strains. .
Descritores: Antígenos de Bactérias/genética
Proteínas de Bactérias/genética
Helicobacter pylori
Infecções por Helicobacter/microbiologia
Neoplasias Gástricas/microbiologia
-Alelos
Motivos de Aminoácidos
Infecções Assintomáticas
Antígenos de Bactérias/metabolismo
Proteínas de Bactérias/metabolismo
Brasil/epidemiologia
Doenças Endêmicas
Detecção Precoce de Câncer/métodos
Genótipo
Helicobacter pylori/genética
Helicobacter pylori/patogenicidade
Fosforilação
Fatores de Risco
População Urbana
Fatores de Virulência/genética
Virulência/genética
Limites: Adolescente
Criança
Feminino
Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-722173
Autor: Kong, X.D.; Liu, N.; Xu, X.J..
Título: Bioinformatics analysis of biomarkers and transcriptional factor motifs in Down syndrome
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(10):834-841, 10/2014. tab, graf.
Idioma: en.
Resumo: In this study, biomarkers and transcriptional factor motifs were identified in order to investigate the etiology and phenotypic severity of Down syndrome. GSE 1281, GSE 1611, and GSE 5390 were downloaded from the gene expression ominibus (GEO). A robust multiarray analysis (RMA) algorithm was applied to detect differentially expressed genes (DEGs). In order to screen for biological pathways and to interrogate the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, the database for annotation, visualization, and integrated discovery (DAVID) was used to carry out a gene ontology (GO) function enrichment for DEGs. Finally, a transcriptional regulatory network was constructed, and a hypergeometric distribution test was applied to select for significantly enriched transcriptional factor motifs. CBR1, DYRK1A, HMGN1, ITSN1, RCAN1, SON, TMEM50B, and TTC3 were each up-regulated two-fold in Down syndrome samples compared to normal samples; of these, SON and TTC3 were newly reported. CBR1, DYRK1A, HMGN1, ITSN1, RCAN1, SON, TMEM50B, and TTC3 were located on human chromosome 21 (mouse chromosome 16). The DEGs were significantly enriched in macromolecular complex subunit organization and focal adhesion pathways. Eleven significantly enriched transcription factor motifs (PAX5, EGR1, XBP1, SREBP1, OLF1, MZF1, NFY, NFKAPPAB, MYCMAX, NFE2, and RP58) were identified. The DEGs and transcription factor motifs identified in our study provide biomarkers for the understanding of Down syndrome pathogenesis and progression.
Descritores: Motivos de Aminoácidos/genética
Biologia Computacional/métodos
Síndrome de Down/genética
Redes Reguladoras de Genes/genética
Fatores de Transcrição/análise
-Algoritmos
Biomarcadores/análise
Bases de Dados Genéticas
Síndrome de Down/etiologia
Expressão Gênica
Ontologia Genética
Anotação de Sequência Molecular/métodos
Fenótipo
Análise Serial de Proteínas/métodos
Regulação para Cima/genética
Limites: Animais
Seres Humanos
Camundongos
Ratos
Responsável: BR1.1 - BIREME


  5 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-709431
Autor: Liu, G.; Li, D.Z.; Jiang, C.S.; Wang, W..
Título: Transduction motif analysis of gastric cancer based on a human signaling network
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(5):369-375, 02/05/2014. tab, graf.
Idioma: en.
Resumo: To investigate signal regulation models of gastric cancer, databases and literature were used to construct the signaling network in humans. Topological characteristics of the network were analyzed by CytoScape. After marking gastric cancer-related genes extracted from the CancerResource, GeneRIF, and COSMIC databases, the FANMOD software was used for the mining of gastric cancer-related motifs in a network with three vertices. The significant motif difference method was adopted to identify significantly different motifs in the normal and cancer states. Finally, we conducted a series of analyses of the significantly different motifs, including gene ontology, function annotation of genes, and model classification. A human signaling network was constructed, with 1643 nodes and 5089 regulating interactions. The network was configured to have the characteristics of other biological networks. There were 57,942 motifs marked with gastric cancer-related genes out of a total of 69,492 motifs, and 264 motifs were selected as significantly different motifs by calculating the significant motif difference (SMD) scores. Genes in significantly different motifs were mainly enriched in functions associated with cancer genesis, such as regulation of cell death, amino acid phosphorylation of proteins, and intracellular signaling cascades. The top five significantly different motifs were mainly cascade and positive feedback types. Almost all genes in the five motifs were cancer related, including EPOR, MAPK14, BCL2L1, KRT18, PTPN6, CASP3, TGFBR2, AR, and CASP7. The development of cancer might be curbed by inhibiting signal transductions upstream and downstream of the selected motifs.
Descritores: Mineração de Dados
Redes Reguladoras de Genes
Transdução de Sinais/genética
Neoplasias Gástricas/genética
-Motivos de Aminoácidos/genética
Morte Celular
Carcinogênese/genética
Retroalimentação Fisiológica
Regulação da Expressão Gênica
Ontologia Genética
Anotação de Sequência Molecular
Fosforilação
Neoplasias Gástricas/metabolismo
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  6 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-685483
Autor: Memorias do Instituto Oswaldo Cruz; Junqueira, Dennis Maletich; Medeiros, Rubia Marilia de; Leite, Thaysse Cristina Neiva Ferreira; Guimaraes, Monick Lindenmeyer; Graf, Tiago; Pinto, Aguinaldo Roberto; Almeida, Sabrina Esteves de Matos.
Título: Detection of the B"-GWGR variant in the southernmost region of Brazil: unveiling the complexity of the human immunodeficiency virus-1 subtype B epidemic
Fonte: Mem. Inst. Oswaldo Cruz;108(6):735-740, set. 2013. tab.
Idioma: en.
Resumo: Typical human immunodeficiency virus-1 subtype B (HIV-1B) sequences present a GPGR signature at the tip of the variable region 3 (V3) loop; however, unusual motifs harbouring a GWGR signature have also been isolated. Although epidemiological studies have detected this variant in approximately 17-50% of the total infections in Brazil, the prevalence of B"-GWGR in the southernmost region of Brazil is not yet clear. This study aimed to investigate the C2-V3 molecular diversity of the HIV-1B epidemic in southernmost Brazil. HIV-1 seropositive patients were ana-lysed at two distinct time points in the state of Rio Grande do Sul (RS98 and RS08) and at one time point in the state of Santa Catarina (SC08). Phylogenetic analysis classified 46 individuals in the RS98 group as HIV-1B and their molecular signatures were as follows: 26% B"-GWGR, 54% B-GPGR and 20% other motifs. In the RS08 group, HIV-1B was present in 32 samples: 22% B"-GWGR, 59% B-GPGR and 19% other motifs. In the SC08 group, 32 HIV-1B samples were found: 28% B"-GWGR, 59% B-GPGR and 13% other motifs. No association could be established between the HIV-1B V3 signatures and exposure categories in the HIV-1B epidemic in RS. However, B-GPGR seemed to be related to heterosexual individuals in the SC08 group. Our results suggest that the established B"-GWGR epidemics in both cities have similar patterns, which is likely due to their geographical proximity and cultural relationship.
Descritores: Infecções por HIV/epidemiologia
Infecções por HIV/transmissão
Soropositividade para HIV/virologia
HIV-1
-Motivos de Aminoácidos
Sequência de Aminoácidos
Transfusão de Sangue/efeitos adversos
Brasil/epidemiologia
Usuários de Drogas/estatística & dados numéricos
Heterossexualidade
HIV-1
Homossexualidade Masculina
Epidemiologia Molecular
Filogenia
Prevalência
Parceiros Sexuais
Alinhamento de Sequência/estatística & dados numéricos
Limites: Feminino
Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  7 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-659490
Autor: Gómez Restrepo, Carlos.
Título: Revista Colombiana de Psiquiatría: diez años de intensa labor y propuestas para el futuro / Revista Colombiana de Psiquiatría: Ten Years of Intense Work and Proposals for the Future
Fonte: Rev. colomb. psiquiatr;41(2):243-247, abr.-jun. 2012.
Idioma: es.
Descritores: Proteínas de Bactérias/genética
Etanol/farmacologia
Regulação Bacteriana da Expressão Gênica
Genes Reguladores
Proteoma/genética
Synechocystis/efeitos dos fármacos
-Adaptação Fisiológica
Motivos de Aminoácidos
Biocombustíveis
Proteínas de Bactérias/metabolismo
Etanol/metabolismo
Proteínas de Choque Térmico/genética
Proteínas de Choque Térmico/metabolismo
Anotação de Sequência Molecular
Dados de Sequência Molecular
Regiões Promotoras Genéticas
Ligação Proteica
Fotossíntese/efeitos dos fármacos
Proteoma/metabolismo
Simportadores de Sódio-Bicarbonato/genética
Simportadores de Sódio-Bicarbonato/metabolismo
Synechocystis/genética
Synechocystis/crescimento & desenvolvimento
Synechocystis/metabolismo
Transcrição Genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CO332 - Facultad de Medicina


  8 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-639942
Autor: Ayala Corredor, Catalina; Solís Castillo, Carolina.
Título: Otras posibles aplicaciones clínicas de fármacos con efecto 5HT2A y 3 en psiquiatría de enlace: reporte de casos / Other Possible Clinical Applications of Drugs with 5HT2A effect in Liaison Psychiatry: Cases Report
Fonte: Rev. colomb. psiquiatr;41(1):217-228, ene.-abr. 2012. ilus, graf, tab.
Idioma: es.
Resumo: Introducción: En psiquiatría de enlace se logra obtener una visión integral del tratamiento y de las necesidades de cada paciente prestando especial atención a las interacciones medicamentosas y a las contraindicaciones. Algunos casos particulares motivaron la descripción, reporte y revisión bibliográfica acerca de otras posibles aplicaciones de fármacos antagonistas de los recetores 5HT2A y 3, particularmente mirtazapina y olanzapina, en síndrome de hiperalgesia, tinitus y leucoencefalopatía multifocal progresiva por virus JC. Método: reporte de casos. Resultados y Conclusiones: Se describen los casos de tres pacientes en los cuales fue necesario usar mirtazapina y olanzapina no solo para el control de los síntomas psiquiátricos (afectivos, comportamentales y trastorno del sueño), sino también como coadyuvantes en las patologías de base de cada paciente. El uso de cualquier medicamento en psiquiatría de enlace debe tener en cuenta el contexto del paciente, la comorbilidad, las contraindicaciones y las interacciones farmacológicas para garantizar un desenlace positivo, además de promover el trabajo multidisciplinario entre especialistas.

Introduction: In liaison psychiatry it is possible to get an integral view of patient's treatment and needs, paying special attention to pharmacological interactions and contraindications. Some particular cases motivated the description, report and review about other possible applications of 5HT2A and 5HT3 antagonist, particularly Mirtazapine and Olanzapine, in hyperalgesia syndrome, tinnitus and Progressive Multifocal Leukoencephalopathy by JC virus. Method: Cases report. Results: We describe 3 cases of patients in which Mirtazapine and Olanzapine were necessary not only to control psychiatric symptoms (affective / behavioral symptoms and insomnia) but to act as adjuvant therapy in axis III diseases. The use of any drug in psychiatry must take in to account the context of the patient, the presence of comorbidity, contraindications and pharmacological interactions so as to grant a positive outcome also promoting the multidisciplinary work between specialists.
Descritores: Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Núcleo Celular/metabolismo
Cisteína/metabolismo
Neurônios/metabolismo
Tiorredoxinas/metabolismo
Fatores de Transcrição/metabolismo
-Motivos de Aminoácidos
Proteínas Adaptadoras de Transdução de Sinal/genética
Linhagem Celular Tumoral
Cisteína/química
Citoplasma/metabolismo
Dissulfetos/química
Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Anotação de Sequência Molecular
Dados de Sequência Molecular
Mutação
Neurônios/citologia
Oxirredução
Mapeamento de Interação de Proteínas
Transdução de Sinais
Transcrição Genética
Tiorredoxinas/genética
Fatores de Transcrição/genética
Limites: Seres Humanos
Tipo de Publ: Research Support, N.I.H., Extramural
Responsável: CO332 - Facultad de Medicina


  9 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-639808
Autor: Torres, Lino E; González, Lidice; Melián, Karelia; Alonso, Jordis; Moreno, Arlenis; Hernández, Mayrín; Reyes, Orlando; Bermúdez, Ludisleydis; Campos, Javier; Pérez-Pérez, Guillermo.
Título: EPIYA motif patterns among Cuban Helicobacter pylori CagA positive strains / Patrón de los motivos EPIYA de cepas cubanas de Helicobacter pylori positivas para CagA
Fonte: Biomédica (Bogotá);32(1):23-31, ene.-mar. 2012. ilus, tab.
Idioma: es.
Resumo: Introduction. It is known that polymorphisms in C-terminal region of CagA influence gastric disease development on Helicobacter pylori infection. Additionally, the geographic distribution of these polymorphisms has been associated with the appearance of more severe gastroduodenal pathologies. Objective. To determine the CagA phosphorylation motifs pattern (EPIYA pattern) in Cuban H. pylori isolates, and to study its association with patient´s pathologies. Materials and methods. DNAs from 95 H. pylori cagA-positive strains were used to amplify the 3´ variable region of cagA gene by PCR using two different strategies. Additionally, new primers were designed to identify either Western or Eastern CagAEPIYA motiftype by PCR. To confirm the PCR results, PCR products from 14 representative isolates were purified and sequenced Results. The distribution of the EPIYA motif found was: 2 AB (2.1 %), 1 AC (1.1 %), 1 BC (1.1 %), 70 ABC (73.6 %), 19 ABCC (20 %), and 2 ABCCC (2.1 %). Sequencing analysis confirmed the PCR classification in the 14 studied strains and showed three strains with unusual nucleotide sequences, not reported before. Distribution of the EPIYA-ABC pattern was equivalent in all pathologies (78.9 % in gastric ulcer, 72.5 % in duodenal ulcer and 72.2 % in non-ulcer dyspepsia). Conclusion. The PCR results using the new primers confirmed that all studied strains carried the Western CagA type. No specific EPIYA motif was associated with peptic ulcer. This is the first report that shows EPIYA motif distribution in H. pylori isolates from the Caribbean region.

Introducción. Se sabe que el polimorfismo en la región C-terminal de la citotoxina asociada al gen A (CagA) influye en el desarrollo de la enfermedad gástrica durante la infección por Helicobacter pylori. Objetivo. Determinar el número y el tipo de patrones de fosforilación de CagA (patrón EPIYA) en aislamientos cubanos de H. pylori, y estudiar su asociación con las enfermedades gástricas. Materiales y métodos. Se empleó el ADN de 95 cepas de H. pylori positivas paraCagA, para amplificar la región 3´ variable del gen cagA por PCR, mediante el empleo de diferentes estrategias. Además, se diseñaron nuevos cebadores para clasificar por PCR los aislamientos según el tipo de CagA, occidental o del este asiático. Los productos de PCR obtenidos de 14 aislamientos representativos se purificaron y secuenciaron para confirmar los resultados de la PCR. Resultados. La distribución de los patrones EPIYA encontrada, fue: 2 AB (2,1 %), 1 AC (1,1 %), 1 BC (1,1 %), 70 ABC (73,6 %), 19 ABCC (20 %), y 2 ABCCC (2,1 %). El análisis de la secuenciación confirmó las clasificaciones hechas por PCR en las 14 cepas estudiadas y demostró tres cepas con secuencias únicas de nucleótídos, no reportadas anteriormente. La distribución del patrón EPIYA-ABC fue equivalente en todas las enfermedades encontradas: 78,9 % en úlcera gástrica, 72,5 % en úlcera duodenal y 72,2 % en dispepsia no ulcerada. Conclusión. La mayoría de los aislamientos cubanos presentaron las combinaciones de motivos EPIYA menos virulentas (ABC). Los resultados del empleo de los nuevos cebadores y el análisis de la secuenciación, confirmaron que todas las cepas estudiadas portaban el gen cagA de tipo occidental. Ninguno de los patrones específicos de EPIYA se asoció con úlcera péptica. Este es el primer reporte que muestra la distribución de los motivos EPIYA en los aislamientos de H. pylori de la región del Caribe.
Descritores: Antígenos de Bactérias/química
Proteínas de Bactérias/química
Gastrite/microbiologia
Infecções por Helicobacter/microbiologia
Helicobacter pylori/genética
Processamento de Proteína Pós-Traducional
Úlcera Péptica/microbiologia
-Motivos de Aminoácidos
Sequência de Aminoácidos
ABATTOIRS' UNTRANSLATED REGIONS
Antígenos de Bactérias/genética
Antígenos de Bactérias/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Cuba/epidemiologia
Primers do DNA
DNA Bacteriano/genética
Dispepsia/microbiologia
Infecções por Helicobacter/epidemiologia
Helicobacter pylori/isolamento & purificação
Helicobacter pylori/patogenicidade
Dados de Sequência Molecular
Fosforilação
Reação em Cadeia da Polimerase
/metabolismo
PROTEIN TYROSINE PHOSPHATASE, NON-RECEPTOR TYPE ABELSON MURINE LEUKEMIA VIRUS/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Virulência
Limites: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CO332 - Facultad de Medicina


  10 / 12 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-638558
Autor: Tan, You-Wen; Ge, Guo-Hong; Zhao, Wei; Gan, Jian-He; Zhao, Yun; Niu, Zhi-Lin; Zhang, Dong-Jun; Chen, Li; Yu, Xue- Jun; Yang, Li-Jun.
Título: YMDD motif mutations in chronic hepatitis B antiviral treatment naïve patients: a multi-center study
Fonte: Braz. j. infect. dis;16(3):250-255, May-June 2012. tab.
Idioma: en.
Projeto: Zhenjiang Municipal Science & Technology Commission.
Resumo: OBJECTIVE: This study aimed to determine the natural prevalence of variants of tyrosine-methionine-aspartic acid-aspartic acid (YMDD) motif in patients with chronic hepatitis B (CHB), and to explore its relation with demographic and clinical features, hepatitis B virus (HBV) genotypes, and HBV DNA levels. METHODS: A total of 1,042 antiviral treatment naïve CHB patients (including with lamivudine [LAM]) in the past year were recruited from outpatient and inpatient departments of six centers from December 2008 to June 2010. YMDD variants were analyzed using the HBV drug resistance line probe assay (Inno-Lipa HBV-DR). HBV genotypes were detected with polymerase chain reaction (PCR) microcosmic nucleic acid cross-ELISA, and HBV deoxyribonucleic acid (DNA) was quantitated with real-time PCR. All serum samples underwent tests for HBV, HCV, and HDV with ELISA. RESULTS: YMDD variants were detected in 23.3% (243/1042) of CHB patients. YMDD mutation was accompanied by L180M mutation in 154 (76.9%) patients. Both wild-type HBV and YMDD variant HBV were present in 231 of 243 patients. Interestingly, 12 patients had only YIDD and/or YVDD variants without wild YMDD motif. In addition, 27.2% (98/359) of HbeAg-positive patients had YMDD mutations, which was higher than that in HbeAg-negative patients (21.2%, 145/683). The incidence of YMDD varied among patients with different HBV genotypes, but the difference was not significant. Moreover, the incidence of YMDD in patients with high HBV DNA level was significantly higher than that in those with low HBV DNA level. CONCLUSION: Mutation of YMDD motif was detectable at a high rate in CHB patients in this study. The incidence of YMDD may be correlated with HBeAg and HBV DNA level.
Descritores: Antivirais/uso terapêutico
Ácido Aspártico/genética
Vírus da Hepatite B/genética
Hepatite B Crônica/tratamento farmacológico
Metionina/genética
Mutação/genética
Tirosina/genética
-Motivos de Aminoácidos/efeitos dos fármacos
Motivos de Aminoácidos/genética
DNA Viral/análise
Genótipo
Vírus da Hepatite B/efeitos dos fármacos
Hepatite B Crônica/virologia
Reação em Cadeia da Polimerase
Limites: Adulto
Feminino
Seres Humanos
Masculino
Tipo de Publ: Estudo Multicêntrico
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



página 1 de 2 ir para página        
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde