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  1 / 21 LILACS  
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Id: biblio-1126572
Autor: de la Puente López, Vivian; Cutiño Jiménez, Ania M; López González, Tania.
Título: Marcadores moleculares para la taxonomía e identificación del género Brucella (Alphaproteobacteria) / Molecular markers for the taxonomy and identification of the genus Brucella (Alphaproteobacteria)
Fonte: Rev. cuba. invest. bioméd;39(1):e336, ene.-mar. 2020. graf.
Idioma: es.
Resumo: Introducción: El género Brucella está incluido en la familia Brucellaceae que pertenece al orden Rhizobiales y es reconocido por su alto grado de patogenicidad. Las bacterias de este género son responsables de la brucelosis, enfermedad que ha sido reportada como una de las zoonosis más importantes a nivel mundial por su incidencia en el ganado y el hombre. Los estudios previos para la clasificación taxonómica del género, se han basado fundamentalmente en el análisis del gen 16S ARNr. Sin embargo, pocas investigaciones se han dirigido a la identificación de marcadores moleculares que distingan a sus miembros de otros grupos de bacterias. Objetivo: Identificar inserciones en secuencias de proteínas conservadas, que pudieran ser utilizados como marcadores moleculares para la taxonomía y diagnóstico de especies del género Brucella. Métodos: Las secuencias homólogas de las proteínas analizadas fueron obtenidas de bases de datos internacionales y, posteriormente, alineadas con el programa ClustalX2, para ello fueron considerados los parámetros sugeridos en la literatura. Resultados: Se identificaron inserciones en las proteínas oxoglutarato deshidrogenasa (componente E1) y ADN ligasa A específicas del género Brucella. Conclusiones: Las inserciones halladas pueden ser empleadas como complemento a los métodos tradicionales de clasificación taxonómica y para el diagnóstico molecular de bacterias incluidas en el género Brucella(AU)

Introduction: Brucella is a genus from the Brucellaceae family, Rhizobiales order. This genus is recognized for its high pathogenicity. Brucella bacteria cause brucellosis, a disease reported as one of the most important zoonoses worldwide due to its incidence in cattle and people. Previous studies on taxonomic classification of the genus have been mainly based on the analysis of gene 16S rDNA. However, few studies have been aimed at identification of molecular markers distinguishing its members from other groups of bacteria. Objective: Identify insertions in preserved protein sequences which could be used as molecular markers for the taxonomy and diagnosis of species from the Brucella genus. Methods: The homologous sequences for the proteins analyzed were obtained from international databases and aligned with the software ClustalX2, considering the parameters suggested in the literature. Results: Insertions were identified in the proteins oxoglutarate dehydrogenase (component E1) and DNA ligase A, specific of the genus Brucella. Conclusions: The insertions found may be used as complements to the traditional methods for taxonomic classification and for the molecular diagnosis of bacteria from the genus Brucella(AU)
Descritores: Homologia de Sequência
Complexo Cetoglutarato Desidrogenase
-Brucella/patogenicidade
Marcadores Genéticos/genética
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


  2 / 21 LILACS  
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Id: biblio-839381
Autor: Martins, Maira de S. N; Castro, Alessandra M. M. G. de; Lima, Michele dos S; Pinto, Vivian da S. C; Silva, Thaís G. da; Fava, Claudia Del; Depes, Claudio Regis; Okuda, Liria H; Pituco, Edviges M.
Título: Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
Fonte: Braz. j. microbiol;48(2):366-372, April.-June 2017. tab, graf.
Idioma: en.
Resumo: Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
Descritores: Filogenia
Técnicas de Diagnóstico Molecular/métodos
Herpesviridae/isolamento & purificação
Febre Catarral Maligna/diagnóstico
Febre Catarral Maligna/patologia
-Brasil
Bovinos
Análise por Conglomerados
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
Homologia de Sequência
Análise de Sequência de DNA
Genótipo
Herpesviridae/classificação
Herpesviridae/genética
Histocitoquímica
Microscopia
Limites: Animais
Responsável: BR1.1 - BIREME


  3 / 21 LILACS  
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Id: biblio-839368
Autor: Passos-Castilho, Ana Maria; Granato, Celso Francisco Hernandes.
Título: High frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolates
Fonte: Braz. j. microbiol;48(2):373-379, April.-June 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Abstract Hepatitis E virus is responsible for acute and chronic liver infections worldwide. Swine hepatitis E virus has been isolated in Brazil, and a probable zoonotic transmission has been described, although data are still scarce. The aim of this study was to investigate the frequency of hepatitis E virus infection in pigs from a small-scale farm in the rural area of Paraná State, South Brazil. Fecal samples were collected from 170 pigs and screened for hepatitis E virus RNA using a duplex real-time RT-PCR targeting a highly conserved 70 nt long sequence within overlapping parts of ORF2 and ORF3 as well as a 113 nt sequence of ORF2. Positive samples with high viral loads were subjected to direct sequencing and phylogenetic analysis. hepatitis E virus RNA was detected in 34 (20.0%) of the 170 pigs following positive results in at least one set of screening real-time RT-PCR primers and probes. The swine hepatitis E virus strains clustered with the genotype hepatitis E virus-3b reference sequences in the phylogenetic analysis and showed close similarity to human hepatitis E virus isolates previously reported in Brazil.
Descritores: Doenças dos Suínos/epidemiologia
Vírus da Hepatite E/isolamento & purificação
Vírus da Hepatite E/classificação
Hepatite E/veterinária
-Filogenia
Suínos
Doenças dos Suínos/virologia
Brasil
RNA Viral/análise
RNA Viral/genética
Análise por Conglomerados
Prevalência
Vírus da Hepatite E/genética
Hepatite E/epidemiologia
Hepatite E/virologia
Homologia de Sequência
Análise de Sequência de DNA
Fezes/virologia
Reação em Cadeia da Polimerase em Tempo Real
Limites: Animais
Responsável: BR1.1 - BIREME


  4 / 21 LILACS  
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Id: biblio-1022299
Autor: Luchs, A; Cilli, A; Morillo, S G; Ribeiro, Cibele Daniel; Carmona, Rita de Cássia C; Timenetsky, M do C S T.
Título: Rotavirus genotypes and the indigenous children of Brazilian midwest in the vaccine era, 2008-2012: footprints of animal genome
Fonte: J. med. virol;87(11):1881-1889, 2015.
Idioma: en.
Resumo: World group A rotavirus (RVA) surveillance data provides useful estimates of the disease burden, however, indigenous population might require special consideration. The aim of this study was to describe the results of G­ and P­types from Brazilian native children ≤3 years. Furthermore, selected strains have been analyzed for the VP7, VP6, VP4, and NSP4 encoding genes in order to gain insight into genetic variability of Brazilian strains. A total of 149 samples, collected during 2008­2012, were tested for RVA using ELISA and PAGE, following by RT­PCR and sequencing. RVA infection was detected in 8.7% of samples (13/149). Genotype G2P[4] was detected in 2008 and 2010, G8P[6] in 2009, and G3P[8] in 2011. The phylogenetic analysis of the VP7 and VP4 genes grouped the Brazilian G2P[4] and G3P[8] strains within the lineages currently circulating in humans worldwide. However, the phylogenetic analysis of the VP6 and NSP4 from the Brazilian G2P[4] strains, and the VP7 and NSP4 from the Brazilian G3P[8] strains suggest a distant common ancestor with different animal strains (bovine, caprine, and porcine). The epidemiological and genetic information obtained in the present study is expected to provide an updated understanding of RVA genotypes circulating in the native infant population, and to formulate policies for the use of RVA vaccines in indigenous Brazilian people. Moreover, these results highlight the great diversity of human RVA strains circulating in Brazil, and an in­depth surveillance of human and animal RVA will lead to a better understanding of the complex dynamics of RVA evolution
Descritores: Filogenia
Infecções por Rotavirus/virologia
Variação Genética
Proteínas Virais/genética
Brasil
Humanos
Ensaio de Imunoadsorção Enzimática
Dados de Sequência Molecular
Pré-Escolar
Reação em Cadeia da Polimerase
Homologia de Sequência
Análise de Sequência de DNA
Rotavirus/isolamento & purificação
Rotavirus/genética
Rotavirus/química
Evolução Molecular
Grupos Populacionais
Genótipo
Lactente
Responsável: BR91.2 - Centro de Documentação


  5 / 21 LILACS  
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Id: biblio-889243
Autor: Biswas, Subhajit; Jackson, Philippa; Shannon, Rebecca; Dulwich, Katherine; Sukla, Soumi; Dixon, Ronald A.
Título: Molecular screening of blue mussels indicated high mid-summer prevalence of human genogroup II Noroviruses, including the pandemic "GII. 4 2012" variants in UK coastal waters during 2013
Fonte: Braz. j. microbiol;49(2):279-284, Apr.-June 2018. graf.
Idioma: en.
Resumo: Abstract This molecular study is the first report, to the best of our knowledge, on identification of norovirus, NoV GII.4 Sydney 2012 variants, from blue mussels collected from UK coastal waters. Blue mussels (three pooled samples from twelve mussels) collected during the 2013 summer months from UK coastal sites were screened by RT-PCR assays. PCR products of RdRP gene for noroviruses were purified, sequenced and subjected to phylogenetic analysis. All the samples tested positive for NoVs. Sequencing revealed that the NoV partial RdRP gene sequences from two pooled samples clustered with the pandemic "GII.4 Sydney variants" whilst the other pooled sample clustered with the NoV GII.2 variants. This molecular study indicated mussel contamination with pathogenic NoVs even during mid-summer in UK coastal waters which posed potential risk of NoV outbreaks irrespective of season. As the detection of Sydney 2012 NoV from our preliminary study of natural coastal mussels interestingly corroborated with NoV outbreaks in nearby areas during the same period, it emphasizes the importance of environmental surveillance work for forecast of high risk zones of NoV outbreaks.
Descritores: Genótipo
Mytilus edulis/virologia
Norovirus/classificação
Norovirus/isolamento & purificação
-Organismos Aquáticos/virologia
Análise por Conglomerados
Programas de Rastreamento
Norovirus/genética
Filogenia
Prevalência
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Polimerase Dependente de RNA/genética
Estações do Ano
Análise de Sequência de DNA
Homologia de Sequência
Reino Unido
Limites: Animais
Responsável: BR1.1 - BIREME


  6 / 21 LILACS  
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Id: biblio-889226
Autor: Thomas, Lebin; Ram, Hari; Singh, Ved Pal.
Título: Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus
Fonte: Braz. j. microbiol;49(2):429-442, Apr.-June 2018. tab, graf.
Idioma: en.
Resumo: Abstract Bacteria are important sources of cellulases with various industrial and biotechnological applications. In view of this, a non-hemolytic bacterial strain, tolerant to various environmental pollutants (heavy metals and organic solvents), showing high cellulolytic index (7.89) was isolated from cattle shed soil and identified as Bacillus sp. SV1 (99.27% pairwise similarity with Bacillus korlensis). Extracellular cellulases showed the presence of endoglucanase, total cellulase and β-glucosidase activities. Cellulase production was induced in presence of cellulose (3.3 times CMCase, 2.9 times FPase and 2.1 times β-glucosidase), and enhanced (115.1% CMCase) by low-cost corn steep solids. An in silico investigation of endoglucanase (EC 3.2.1.4) protein sequences of three Bacillus spp. as query, revealed their similarities with members of nine bacterial phyla and to Eukaryota (represented by Arthropoda and Nematoda), and also highlighted of a convergent and divergent evolution from other enzymes of different substrate [(1,3)-linked beta-d-glucans, xylan and chitosan] specificities. Characteristic conserved signature indels were observed among members of Actinobacteria (7 aa insert) and Firmicutes (9 aa insert) that served as a potential tool in support of their relatedness in phylogenetic trees.
Descritores: Bacillus/enzimologia
Celulase/genética
Celulase/metabolismo
Evolução Molecular
-Bacillus/crescimento & desenvolvimento
Bacillus/isolamento & purificação
Celulose/metabolismo
Biologia Computacional
Fezes/microbiologia
Regulação Bacteriana da Expressão Gênica
Regulação Enzimológica da Expressão Gênica
Mutação INDEL
Análise de Sequência de DNA
Homologia de Sequência
Especificidade por Substrato
Zea mays/metabolismo
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


  7 / 21 LILACS  
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Id: lil-775121
Autor: Jan, Arif Tasleem; Azam, Mudsser; Choi, Inho; Ali, Arif; Haq, Qazi Mohd. Rizwanul.
Título: Analysis for the presence of determinants involved in the transport of mercury across bacterial membrane from polluted water bodies of India
Fonte: Braz. j. microbiol;47(1):55-62, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Mercury, which is ubiquitous and recalcitrant to biodegradation processes, threatens human health by escaping to the environment via various natural and anthropogenic activities. Non-biodegradability of mercury pollutants has necessitated the development and implementation of economic alternatives with promising potential to remove metals from the environment. Enhancement of microbial based remediation strategies through genetic engineering approaches provides one such alternative with a promising future. In this study, bacterial isolates inhabiting polluted sites were screened for tolerance to varying concentrations of mercuric chloride. Following identification, several Pseudomonas and Klebsiella species were found to exhibit the highest tolerance to both organic and inorganic mercury. Screened bacterial isolates were examined for their genetic make-up in terms of the presence of genes (merP and merT) involved in the transport of mercury across the membrane either alone or in combination to deal with the toxic mercury. Gene sequence analysis revealed that the merP gene showed 86–99% homology, while the merT gene showed >98% homology with previously reported sequences. By exploring the genes involved in imparting metal resistance to bacteria, this study will serve to highlight the credentials that are particularly advantageous for their practical application to remediation of mercury from the environment.
Descritores: Klebsiella/metabolismo
Proteínas de Membrana Transportadoras/genética
Proteínas de Membrana Transportadoras/metabolismo
Mercúrio/metabolismo
Pseudomonas/metabolismo
Poluentes Químicos da Água/metabolismo
-Tolerância a Medicamentos
Genes Bacterianos
Índia
Klebsiella/efeitos dos fármacos
Klebsiella/genética
Dados de Sequência Molecular
Mercúrio/toxicidade
Pseudomonas/efeitos dos fármacos
Pseudomonas/genética
Análise de Sequência de DNA
Homologia de Sequência
Poluentes Químicos da Água/toxicidade
Limites: Humanos
Responsável: BR1.1 - BIREME


  8 / 21 LILACS  
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Id: lil-742762
Autor: ACQUAFRESCA, Pablo A.; PALERMO, Mariano; ROGULA, Tomasz; DUZA, Guillermo E.; SERRA, Edgardo.
Título: Early surgical complications after gastric by-pass: a literature review / Complicações cirúrgicas precoces após bypass gástrico: revisão da literatura
Fonte: ABCD arq. bras. cir. dig;28(1):74-80, 2015. tab, graf.
Idioma: en.
Resumo: INTRODUCTION: Gastric bypass is today the most frequently performed bariatric procedure,but, despite of it, several complications can occur with varied morbimortality. Probably all bariatric surgeons know these complications, but, as bariatric surgery continues to spread, general surgeon must be familiarized to it and its management. Gastric bypass complications can be divided into two groups: early and late complications, taking into account the two weeks period after the surgery. This paper will focus the early ones. METHOD: Literature review was carried out using Medline/PubMed, Cochrane Library, SciELO, and additional information on institutional sites of interest crossing the headings: gastric bypass AND complications; follow-up studies AND complications; postoperative complications AND anastomosis, Roux-en-Y; obesity AND postoperative complications. Search language was English. RESULTS: There were selected 26 studies that matched the headings. Early complications included: anastomotic or staple line leaks, gastrointestinal bleeding, intestinal obstruction and incorrect Roux limb reconstruction. CONCLUSION: Knowledge on strategies on how to reduce the risk and incidence of complications must be acquired, and every surgeon must be familiar with these complications in order to achieve an earlier recognition and perform the best intervention. .

INTRODUÇÃO: O bypass gástrico é hoje o procedimento bariátrico mais realizado, mas, apesar disso, várias complicações podem ocorrer com variada morbimortalidade. Provavelmente todos os cirurgiões bariátricos conhecem essas complicações, mas como a cirurgia bariátrica continua a se espalhar, o cirurgião geral deve estar familiarizado com essas complicações e seu manuseio. As complicações do bypass gástrico podem ser divididas em dois grupos: as precoces e tardias, tendo em conta o período de duas semanas após a operação. Este artigo irá focar as precoces. MÉTODO: Foi realizada revisão da literatura utilizando as bases Medline/PubMed, Cochrane Library, SciELO, e informações adicionais sobre sites institucionais de interesse cruzando os descritores: bypass gástrico AND complicações; seguimento AND complicações; complicações pós-operatórias AND anastomose, Roux-en-Y; obesidade AND complicações pós-operatórias. A língua usada para a busca foi o inglês. RESULTADOS: Foram selecionados 26 artigos que combinavam com os descritores. As complicações imediatas foram: fístula na linha de grampeamento, sangramento gastrointestinal, obstrução intestinal e reconstrução incorreta da alça em Roux. CONCLUSÃO: O conhecimento sobre as estratégias de como reduzir o risco e incidência das complicações deve ser adquirido ao longo do tempo, e cada cirurgião deve estar familiarizado com essas complicações, a fim de reconhecê-las precocemente e realizar a melhor intervenção. .
Descritores: Linfócitos B/fisiologia
Poli(ADP-Ribose) Polimerases/fisiologia
-Formação de Anticorpos/efeitos dos fármacos
Formação de Anticorpos/genética
Apoptose/genética
Apoptose/imunologia
Linfócitos B/efeitos dos fármacos
Linfócitos B/metabolismo
Diferenciação Celular/efeitos dos fármacos
Diferenciação Celular/genética
Sobrevivência Celular/genética
Imunoglobulina A/imunologia
/farmacologia
INTERLEUKIN-ABBREVIATIONS AS TOPIC/farmacologia
Camundongos Knockout
Família Multigênica
MICE, INBRED CABDOMENABDOMINAL INJURIESBL
Proteínas de Neoplasias/genética
Proteínas de Neoplasias/metabolismo
Proteínas de Neoplasias/fisiologia
Poli(ADP-Ribose) Polimerases/química
Poli(ADP-Ribose) Polimerases/genética
Poli(ADP-Ribose) Polimerases/metabolismo
Homologia de Sequência
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  9 / 21 LILACS  
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Id: lil-741309
Autor: Lilenbaum, Walter; Kremer, Frederico; Ristow, Paula; Dellagostin, Odir; Bourhy, Pascale; Hartskeerl, Rudy; Vasconcellos, Silvio.
Título: Molecular characterization of the first leptospires isolated from goats in Brazil
Fonte: Braz. j. microbiol;45(4):1527-1530, Oct.-Dec. 2014. ilus.
Idioma: en.
Resumo: Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca.
Descritores: Infecções Assintomáticas
Leptospira/isolamento & purificação
Leptospirose/veterinária
-Sequência de Bases
Brasil
Proteínas de Bactérias/genética
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
RNA Polimerases Dirigidas por DNA/genética
Cabras
Leptospira/classificação
Leptospira/genética
Leptospirose/microbiologia
Dados de Sequência Molecular
Análise de Sequência de DNA
Homologia de Sequência
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  10 / 21 LILACS  
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Id: lil-726799
Autor: Gunturiz, María Luz; Gómez, Luis Alberto.
Título: Identificación y expresión diferencial del factor de transcripción asociado a microftalmia en corazón y cardiomiocitos aislados de cobayo: su posible papel en la hipertrofia y la viabilidad / Identification and differential expression of the microphthalmia-associated transcription factor in heart and isolated cardiomyocytes from Guinea pigs: Possible role in hypertrophy and viability
Fonte: Biomédica (Bogotá);34(3):387-402, July-Sept. 2014. ilus.
Idioma: es.
Resumo: Introducción. El factor de transcripción asociado a la microftalmia ( Microphtalmia-Associated Transcription Factor , MITF) regula la expresión de genes específicos, pero no se conoce su expresión y su función a nivel cardiaco. Objetivos. Identificar la expresión del MITF en corazón y en cardiomiocitos aislados de cobayo, describir los cambios morfológicos asociados con su disminución y evaluar los niveles relativos de su expresión en cardiomiocitos aislados en condiciones de preacondicionamiento isquémico. Materiales y métodos. El análisis de la expresión relativa de la isoforma específica de tejido cardiaco ( heart-type MITF, MITF-H), se determinó mediante reacción en cadena de la polimerasa (PCR) en tiempo real semicuantitativa, secuenciación y Western blot . La disminución del ARNm del MITF se indujo con un ARN pequeño de interferencia ( short hairpin RNA interference , shRNAi) específico. El tamaño, el diámetro y el número de fibras musculares se evaluaron por observación directa con microscopía de luz. Resultados. Se amplificó un fragmento de 281 pb de ADNc; el análisis de la secuencia confirmó la identidad del exón 1 y la isoforma H del MITF. La interferencia del ARNm del MITF se asoció con un mayor índice cardiaco (peso corazón/peso corporal: 5,46 x 10 -3 Vs. 4,6 x 10 -3 ) y un incremento del diámetro de las fibras cardiacas (50,2±16 µm Vs. 38,7±14,7 µm; p<0,05, n=150). En los cardiomiocitos aislados en condiciones de preacondicionamiento isquémico, se observó una expresión relativa del MITF-H mayor que en los miocitos en normoxia y expuestos a lesión por isquemia simulada (80 y 100 veces más, n=5, p<0,05, n=3). Conclusión. Los resultados sugieren que el MITF-H podría estar involucrado en la hipertrofia, la respuesta al estrés por isquemia y la supervivencia de cardiomiocitos de cobayo.

Introduction: The microphthalmia -associated transcription factor ( MITF ) regulates the expression of specific genes and its cardiac expression and function is not known. Objectives: To identify the expression of MITF in hearts and isolated cardiomyocytes from Guinea pigs, to describe morphological changes associated with mRNA interference of MITF and to evaluate their relative changes in expression in isolated cardiomyocytes under ischemic preconditioning. Materials and methods: The cardiac specific isoform, MITF-H, and relative expression level analysis, was determined by semi-quantitative real time PCR, sequencing and Western blotting. Reduction of mRNA-MITF-H was induced by transduction of specific-MITF-shRNAi interference. The cardiac morphological changes, diameter and number of cardiac fibers were evaluated by direct observation and light microscopy. Results: A cDNA fragment of 281 bp was amplified from heart and isolated ventricular cardiac myocytes. Sequence analysis confirmed the identity of the isoform MITF-H, exon 1. The MITF silencing was associated with an increase in cardiac index (heart weight/body weight vs . 5.46 x 10 -3 vs 4.6 x 10 -3 ) and higher diameter of cardiac fibers (50.2±16 µ m vs 38,7±14,7 µ m p<0.05, n=150). In isolated cardiac myocytes under ischemic preconditioning we observed a higher relative expression compared with that measured in myocytes exposed to normoxia and simulated ischemia (eighty and one hundred times, p <0.05, n = 5). Conclusion. The results suggest that MITF-H isoform may be involved in Guinea pig cardiac hypertrophy, response to stress by ischemia and cardiomyocytes survival.
Descritores: Cardiomiopatia Hipertrófica/metabolismo
Fator de Transcrição Associado à Microftalmia/fisiologia
Miocárdio/metabolismo
Miócitos Cardíacos/metabolismo
-Sequência de Aminoácidos
Sequência de Bases
Sobrevivência Celular
Células Cultivadas
Cardiomiopatia Hipertrófica/genética
DNA Complementar/genética
Regulação da Expressão Gênica
Precondicionamento Isquêmico Miocárdico
Dados de Sequência Molecular
Fator de Transcrição Associado à Microftalmia/antagonistas & inibidores
Fator de Transcrição Associado à Microftalmia/biossíntese
Fator de Transcrição Associado à Microftalmia/genética
Isquemia Miocárdica/genética
Isquemia Miocárdica/metabolismo
Miócitos Cardíacos/patologia
Oxigênio/farmacologia
Isoformas de Proteínas/biossíntese
Isoformas de Proteínas/genética
Isoformas de Proteínas/fisiologia
Interferência de RNA
RNA Interferente Pequeno/farmacologia
Alinhamento de Sequência
Homologia de Sequência
Limites: Animais
Feminino
Cobaias
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CO332 - Facultad de Medicina



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