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Pesquisa : G02.111.810.200 [Categoria DeCS]
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Id: lil-752510
Autor: Teixeira, Carla Regina de Souza; Pereira, Marta Cristiane Alves; Kusumota, Luciana; Gaioso, Vanessa Pirani; Mello, Carolina Lima de; Carvalho, Emília Campos de.
Título: Avaliação dos estudantes de enfermagem sobre a aprendizagem com a simulação clínica / Evaluación de los estudiantes de enfermería sobre el aprendizaje con la simulación clínica / Evaluation of nursing students about learning with clinical simulation
Fonte: Rev. bras. enferm;68(2):311-319, Mar-Apr/2015. tab.
Idioma: pt.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: RESUMO Objetivo: descrever as contribuições da simulação clínica para aprendizagem de atributos cognitivos e procedimentais, por meio do debriefing, na perspectiva dos estudantes de enfermagem. Método: estudo descritivo exploratório. Participaram 20 estudantes de Graduação em Enfermagem de uma universidade do interior paulista. Na coleta de dados, realizada na etapa do debriefing, foi registrada a percepção do aluno sobre a simulação, aspectos positivos e o que poderia ser feito de forma diferente. Os relatos foram agrupados em categorias temáticas centrais, segundo referencial de análise de conteúdo de Bardin (2011), analisadas por meio de estatística descritiva. Resultados: identificada valorização da aprendizagem ativa, crítica e reflexiva (47,5%) em decorrência da aproximação à realidade assistencial (20,3%), manifestação dos sentimentos vivenciados durante a simulação (16,9%) e composição do cenário (15,3%). Conclusão: a simulação clínica seguida do debriefing favorece a compreensão da relação entre ação e resultados alcançados na aprendizagem. .

RESUMEN Objetivo: describir las contribuciones de simulación clínica para aprender atributos cognitivos y de procedimiento, a través de debriefing, desde la perspectiva de los estudiantes de enfermería. Método: estudio exploratorio descriptivo. 20 estudiantes participaron en el Pregrado en Enfermería de una universidad de São Paulo. Durante la recolección de datos, que se aplicó durante el debriefing, fue grabado en la percepción de los estudiantes de la simulación, los aspectos positivos y lo que podría hacerse de otra manera. Los informes de los estudiantes se agrupan de acuerdo a los temas centrales, según el referencial de análisis de contenido de Bardin (2011) y analizados mediante estadística descriptiva. Resultados: identificado la mejora de aprendizaje activo, crítico y reflexivo (47,5%) debido a la aproximación a la realidad en la atención de enfermería (20,3%), un resultado de la composición del escenario (16,9%), lo que favorece el desarrollo de sentimientos experimentados durante la simulación (15,3%). Conclusión: la simulación clínica seguida de debriefing favorece la comprensión de la relación entre la acción y los resultados obtenidos en el aprendizaje. .

ABSTRACT Objective: to describe the contributions of clinical simulation for learning cognitive and procedural attributes through debriefi ng, from the perspective of nursing students. Method: descriptive exploratory study. Twenty nursing undergraduate students from a university in the interior of the state of São Paulo participated in this study. Data collection was performed at the debriefi ng stage. Student’s perceptions about the simulation, positive aspects and what they could have done differently were registered. The students’ statements were grouped according to the central themes and the framework of Bardin’s content analysis (2011) and were analyzed using descriptive statistics. Results: enhancement of active, critical and refl ective learning (47.5%) was identifi ed due to the closeness to reality in nursing care (20.3%), manifestation of feelings experienced during the simulation (15.3%) and composition of the scenario (15.3%). Conclusion: the clinical simulation followed by debriefi ng promotes the understanding of the link between action and achievements in learning. .
Descritores: Proteínas de Arabidopsis/metabolismo
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/imunologia
Imunidade Inata/imunologia
Fragmentos de Peptídeos/imunologia
Imunidade Vegetal/imunologia
Receptores de Reconhecimento de Padrão/imunologia
-Sequência de Aminoácidos
Arabidopsis/genética
Western Blotting
Regulação da Expressão Gênica de Plantas
Dados de Sequência Molecular
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/imunologia
Raízes de Plantas/metabolismo
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Receptores de Reconhecimento de Padrão/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência de Aminoácidos
Transdução de Sinais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-618186
Autor: Wei, C. B; Chen, J.
Título: A novel lipocalin homologue from the venom gland of Deinagkistrodon acutus similar to mammalian lipocalins
Fonte: J. venom. anim. toxins incl. trop. dis;18(1):16-23, 2012. ilus.
Idioma: en.
Projeto: Natural Scientific Foundation of Anhui Province Education Commission.
Resumo: Lipocalins are involved in a variety of functions including retinol transport, cryptic coloration, olfaction, pheromone transport, prostaglandin synthesis, regulation of the immune response and cell homeostatic mediation. A full-length cDNA clone (named d-lipo), isolated from the venom gland cDNA library of Deinagkistrodon acutus, contained an insert of 664 bp including an open reading frame that encodes a lipocalin homologue of 177 amino acids. Comparison of d-lipo and other related proteins revealed an overall amino acid identity of less than 21.5 percent. Primary structures of d-lipo carried three structurally conserved regions (SCR) showing homologies to those of lipocalins. The first conserved Cys residue - the essential amino acid residue for the catalytic activity and unique to lipocalin-type prostaglandin D synthase (L-PGDS) in the lipocalin protein family - was identified in d-lipo at amino acid position 58. Phylogenetic tree analysis showed that d-lipo was in-between the large L-PGDS cluster and the small von Ebner's-gland proteins (VEGP) cluster. Moreover, d-lipo gene presented a high-level expression in the venom gland and a low-level expression in the brain and its expression was significantly increased under pathological conditions, suggesting a possible relationship between d-lipo mRNA expression and the venom gland inflammatory disease. This is also the first report of a lipocalin homologous gene identified in the venom gland of a snake.(AU)
Descritores: Venenos de Serpentes
Homologia de Sequência de Aminoácidos
Lipocalinas/química
-RNA Mensageiro
Biblioteca Gênica
Análise de Sequência de DNA
Limites: Animais
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-1022119
Autor: Luchs, A; Timenetsky, M do C S T.
Título: Phylogenetic analysis of human group C rotavirus circulating in Brazil reveals a potential unique NSP4 genetic variant and high similarity with Asian strains
Fonte: Mol Genet Genomics;290(3):969-986, 2015.
Idioma: en.
Resumo: Group C rotaviruses (RVC) cause gastroenteritis in humans and animals worldwide, and the evidence for a possible zoonotic role has been recently provided. To gain information on the genetic diversity and relationships between human and animal RVC, we sequenced the VP4, VP7, and NSP4 genes of 12, 19, and 15 human strains, respectively, detected in São Paulo state during historical (1988 and 1993) and recent (2007 and 2008) Brazilian rotavirus surveillance. All RVC strains analyzed in the present study grouped into human genotype (G4-P[2]-E2), and did not show any evidence of animal ancestry. Phylogenetic analysis showed that RVC samples detected in 1988 and 1993 clustered together with strains from distinct continents, indicating that historical RVC strains circulating in São Paulo were closely related to those strains circulating worldwide. All three genes (VP7, VP4 and NSP4) of São Paulo RVC strains isolated in 2007-2008 exhibited close phylogenetic relationship with human RVC strains isolated in China and Japan, suggesting that they are genetically linked, and that a gene flow could be occurring between this Asian countries and Brazil. We identified two distinct clusters in the NSP4 phylogenetic tree. One cluster formed exclusively by human Brazilian strains detected in 1997 and 2003-2004 in Rio de Janeiro, Bahia, and Rio Grande do Sul states (Subgroup II) previously described in a different study, that displayed low sequence identities to other human strains formerly published, and to the Brazilian RVC strains (Subgroup I) characterized in the present study. These data suggests the circulation of two genetic profiles of the NSP4 gene in Brazil. High sequence diversity in NSP4 gene was previously reported in Asia, and additional diversity in NSP4 RVC strains spreading in the world should be expected. More in-depth molecular and epidemiological analysis of human RVC throughout the world will be needed to understand their diversity and clarify their evolution, as well as to develop classifications schemes.
Descritores:
Filogenia
Infecções por Rotavirus/virologia
Toxinas Biológicas/genética
Variação Genética
Brasil/epidemiologia
Humanos
RNA
RNA Viral/isolamento & purificação
Dados de Sequência Molecular
Sequência de Bases
Glicoproteínas
Glicoproteínas/genética
Glicoproteínas/química
Criança
Pré-Escolar
Demografia
Alinhamento de Sequência
Adolescente
Sequência de Aminoácidos
Proteínas não Estruturais Virais
Proteínas não Estruturais Virais/genética
Homologia de Sequência de Aminoácidos
Análise de Sequência de DNA
Rotavirus
Adulto
Proteínas do Capsídeo/química
Gastroenterite/virologia
Genótipo
Lactente
Animais
Pessoa de Meia-Idade
Antígenos Virais/genética
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-889234
Autor: Toufiq, Nida; Tabassum, Bushra; Bhatti, Muhammad Umar; Khan, Anwar; Tariq, Muhammad; Shahid, Naila; Nasir, Idrees Ahmad; Husnain, Tayyab.
Título: Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Fonte: Braz. j. microbiol;49(2):414-421, Apr.-June 2018. tab, graf.
Idioma: en.
Resumo: Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I gene was isolated from the genomic DNA of Barley (Hordeum vulgare L.) cultivar, Haider-93. The isolated DNA was used as template for the amplification of the ∼935 bp full-length chitinase I gene. Based on the sequence of the amplified gene fragment, class I barley chitinase shares 93% amino acid sequence homology with class II wheat chitinase. Interestingly, barley class I chitinase and class II chitinase do not share sequence homology. Furthermore, the amplified fragment was expressed in Escherichia coli Rosetta strain under the control of T7 promoter in pET 30a vector. Recombinant chitinase protein of 35 kDa exhibited highest expression at 0.5 mM concentration of IPTG. Expressed recombinant protein of 35 kDa was purified to homogeneity with affinity chromatography. Following purification, a Western blot assay for recombinant chitinase protein measuring 35 kDa was developed with His-tag specific antibodies. The purified recombinant chitinase protein was demonstrated to inhibit significantly the important phytopathogenic fungi Alternaria solani, Fusarium spp, Rhizoctonia solani and Verticillium dahliae compared to the control at concentrations of 80 µg and 200 µg.
Descritores: Antifúngicos/farmacologia
Quitinases/farmacologia
Hordeum/enzimologia
Proteínas Recombinantes/metabolismo
-Antifúngicos/química
Antifúngicos/isolamento & purificação
Western Blotting
Quitinases/química
Quitinases/genética
Quitinases/isolamento & purificação
Cromatografia de Afinidade
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Hordeum/genética
Peso Molecular
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Homologia de Sequência de Aminoácidos
Responsável: BR1.1 - BIREME


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Id: lil-749724
Autor: Mori, Enio; Lara, Maria do Carmo C.S.H.; Cunha, Elenice M.S.; Villalobos, Eliana M.C.; Mori, Claudia M.C.; Soares, Rodrigo M.; Brandão, Paulo E.; Fernandes, Wilson R.; Richtzenhain, Leonardo J..
Título: Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
Fonte: Braz. j. microbiol;46(2):565-570, Apr-Jun/2015. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation; . National Council for Scientific and Technological Development.
Resumo: Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1 archival strains (A3/97, A4/72, A9/92) and three clinical samples (Iso07/05, Iso11/06 and Iso33/06) suggested that among Brazilian EHV-1 strains, the amplified region of the gD gene sequence is highly conserved. Additionally, the analysis of ICP4 gene showed high nucleotide and amino acid identities when compared with genotype P strains, suggesting that the EHV-1 Brazilian strains belonged to the same group. All the EHV-1 Brazilian strains were classified as non-neuropathogenic variants (N752) based on the ORF30 analysis. These findings indicate a high conservation of the gD-, ICP4- and ORF30-encoding sequences. Different pathotypes of the EHV-1 strain might share identical genes with no specific markers, and tissue tropism is not completely dependent on the gD envelope, immediate-early ICP4 and DNA polymerase proteins.
Descritores: Variação Genética
Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1/classificação
Herpesvirus Equídeo 1/genética
Doenças dos Cavalos/virologia
-Brasil
Análise por Conglomerados
Sequência Conservada
DNA Viral/química
DNA Viral/genética
Genótipo
Cavalos
Infecções por Herpesviridae/virologia
Dados de Sequência Molecular
Fases de Leitura Aberta
Filogenia
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Homologia de Sequência do Ácido Nucleico
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-748256
Autor: Gomaa, Ola M.; Momtaz, Osama A..
Título: Copper induction and differential expression of laccase in Aspergillus flavus
Fonte: Braz. j. microbiol;46(1):285-292, 05/2015. tab, graf.
Idioma: en.
Resumo: Aspergillus flavus was isolated from soil and exhibited laccase activity under both constitutive and copper induced conditions. Spiking the medium with 1 mM copper sulfate resulted in an increase in the activity which reached 51.84 U/mL, a distinctive protein band was detected at 60 kDa. The extracellular enzyme was purified 81 fold using gel filtration chromatography and resulted in two different laccase fractions L1 and L2, the latter had a higher enzymatic activity which reached 79.57 U/mL and specific activity of 64.17 U/μg protein. The analysis of the spectrum of the L2 fraction showed a shoulder at 330 nm which is characteristic for T2/T3 copper centers; both copper and zinc were detected suggesting that this is an unconventional white laccase. Primers of laccase gene were designed and synthesized to recover specific gene from A. flavus. Sequence analysis indicated putative laccase (Genbank ID: JF683612) at the amino acid level suggesting a close identity to laccases from other genera containing the copper binding site. Decolorization of textile waste water under different conditions showed possible application in bioremediation within a short period of time. The effect of copper on A. flavus was concentration dependent.
Descritores: Aspergillus flavus/efeitos dos fármacos
Aspergillus flavus/enzimologia
Cobre/metabolismo
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Lacase/biossíntese
Ativação Transcricional/efeitos dos fármacos
-Aspergillus flavus/genética
Aspergillus flavus/isolamento & purificação
Cromatografia em Gel
Meios de Cultura/química
DNA Fúngico/genética
Eletroforese em Gel de Poliacrilamida
Resíduos Industriais
Lacase/química
Lacase/isolamento & purificação
Dados de Sequência Molecular
Peso Molecular
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Microbiologia do Solo
Análise Espectral
Purificação da Água
Responsável: BR1.1 - BIREME


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Id: lil-747152
Autor: AlNoury, Mohammed K.; Almuhayawi, Saad M.; Alghamdi, Khalid B.; Al-Noury, Khaled I..
Título: Preoperative Imaging Modalities to Predict the Risk of Regional Nodal Recurrence in Well-Differentiated Thyroid Cancers
Fonte: Int. arch. otorhinolaryngol. (Impr.);19(2):116-120, Apr-Jun/2015. tab.
Idioma: en.
Resumo: Introduction Thyroid cancer incidence has increased in the previous 2 decades. Preoperative identification of lymph node metastasis is a suggested risk factor associated with recurrence following thyroidectomy. Objectives We aimed to evaluate the accuracy of preoperative radiologic investigations of nodal status in determining the postoperative risk of regional nodal recurrence in cases of well-differentiated thyroid cancer. Methods This is a case series. We retrospectively reviewed data, including preoperative ultrasonography and/or computed tomography results, on patients who underwent total thyroidectomy for thyroid cancer at our hospital between 2006 and 2012. Prognostic factors for predicting recurrence, including age, sex, tumor diameter, and nodal diameter, were evaluated. Results Total thyroidectomy was performed on 24 male and 74 female patients (median age, 43 years). The median follow-up time was 21 months. Sixty-eight patients had papillary thyroid cancer, and 30 had follicular cancer. Nodal recurrence was evident in 30% of patients, and 4% of patients died. Identification of lymph node involvement during preoperative radiologic investigations was strongly prognostic for recurrence: 35.3% of patients with positive preoperative ultrasonography findings and 62.5% of those with positive preoperative computed tomography findings had recurrence (p = 0.01). Conclusions Preoperative identification of lymph node metastasis on radiologic studies was correlated with an increased risk of regional nodal recurrence in well-differentiated thyroid cancer. Computed tomography was superior to ultrasonography in detecting metastatic nodal involvement preoperatively and is therefore recommended for preoperative assessment and postoperative follow-up. .
Descritores: Hematopoese/genética
Leucemia Mieloide Aguda/genética
Proteínas de Peixe-Zebra/fisiologia
Peixe-Zebra/fisiologia
/fisiologia
FMS-LIKE TYROSINE KINASE ABATTOIRS/fisiologia
-Sequência de Aminoácidos
Animais Geneticamente Modificados
Sequência Conservada
Embrião não Mamífero
Dados de Sequência Molecular
Estrutura Terciária de Proteína/genética
Homologia de Sequência de Aminoácidos
Sequências de Repetição em Tandem
Transcriptoma
Proteínas de Peixe-Zebra/química
Peixe-Zebra/embriologia
/química
FMS-LIKE TYROSINE KINASE ABATTOIRS/química
Limites: Animais
Humanos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-746683
Autor: Rovira Forns, Joan.
Título: Precios de los medicamentos: cómo se establecen y cuáles son sus sistemas de control / Drug prices: how they are established and existing price control systems
Fonte: Salud colect;11(1):35-48, ene.-mar. 2015.
Idioma: es.
Resumo: El precio es una de las principales barreras de acceso a los medicamentos. Por ello es importante conocer cómo se forman los precios y qué factores determinan su cuantía y también qué formas de intervención y regulación son las más adecuadas teniendo en cuenta sus efectos, tanto sobre el acceso, como sobre la innovación, la producción local y otros posibles objetivos de la política de medicamentos. El análisis económico ha desarrollado un conjunto de modelos de mercado que permiten explicar el comportamiento de los precios, aunque los mercados reales divergen sustancialmente de los modelos teóricos. La regulación de precios está justificada por los llamados "fallos de mercado"; la regulación de precios basada en el costo de producción, la modalidad de control de precios más tradicional, ha caído en desuso a favor de los sistemas de precios de referencia internacionales y por la fijación del precio basada en el valor.

Price is one of the main barriers of access to medicines. It is therefore important to understand how prices are formed and what factors determine the amount, as well as what interventions and regulations are the most appropriate considering their effects on access, innovation, local production and other potential objectives of drug policy. Economic analysis has developed a set of market models that can explain the behavior of prices, although actual markets diverge substantially from the theoretical models. Price regulation is justified by the so-called "market failures." Price regulation based on the cost of production, the most traditional form of price control, has fallen into disuse in favor of systems of international reference pricing and value-based pricing.
Descritores: /química
ABATTOIRS',ABDOMEN'-CYCLIC-AMP PHOSPHODIESTERASES/química
/metabolismo
ABATTOIRS',ABDOMEN'-CYCLIC-AMP PHOSPHODIESTERASES/metabolismo
Drosophila melanogaster/enzimologia
Regulação Enzimológica da Expressão Gênica/fisiologia
-Sequência de Aminoácidos
Domínio Catalítico
Sequência Conservada
Evolução Molecular
Dados de Sequência Molecular
Homologia de Sequência de Aminoácidos
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-742570
Autor: Pulgar B, Dahiana; Jara C, Aquiles; González V, Gilberto; González D, Hernán.
Título: Tratamiento quirúrgico del hiperparatiroidismo asociado a insuficiencia renal crónica: Experience in 71 patients / Surgical treatment of renal hyperparathyroidism
Fonte: Rev. méd. Chile;143(2):190-196, feb. 2015. ilus, tab.
Idioma: es.
Resumo: Background: Surgery is an effective method for the management of renal hyperparathyroidism. Aim: To report the clinical presentation and results of surgical treatment of renal hyperparathyroidism. Material and Methods: Retrospective analysis of 58 patients aged 46 ± 11 years with secondary hyperparathyroidism (HPT2) and 13 patients aged 53 ± 11 years with tertiary hyperparathyroidism (HPT3), operated at a clinical hospital. Results: In 55 cases (77.4%) the indications for surgery were complications of excess parathyroid hormone (PTH) and in 16 patients (22.6%) a failure of medical treatment. Total parathyroidectomy with intraoperative measurement of PTH (PTHop) plus a forearm parathyroid autograft was performed in 54 (93.1%) patients with HPT2 and in all patients with HPT3. PTHop decreased ≥ 75% in 51 patients (88%) with HPT2 and in 9 patients (69.2%) with HPT3, respectively. Cure of the disease was achieved in 52 (89.7%) and 11 (84.6%) patients with HPT2 and 3, respectively. Median follow-up was 41 months. Five (9.6%) patients with HPT2 and two patients (18.2%) with HPT3 had a recurrence of the disease. Conclusions: In patients with renal hyperparathyroidism, the primary indication for surgery was the presence of complications of PTH excess. A drop in PTHop ≥ 75% from baseline predicts healing in 98% and 100% of cases with secondary or tertiary HPT respectively. Surgery was a safe and effective treatment in both groups.
Descritores: Espessura Intima-Media Carotídea
Desidrogenases de Carboidrato/genética
Polimorfismo de Nucleotídeo Único
-Sequência de Aminoácidos
Aterosclerose/genética
Saúde da Família
Frequência do Gene
Genótipo
Predisposição Genética para Doença/genética
HEKABORTION, INCOMPLETEABATTOIRS CELLS
Modelos Lineares
Dados de Sequência Molecular
Fatores de Risco
Homologia de Sequência de Aminoácidos
Limites: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Landgraf, Mariza
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Id: lil-741311
Autor: Furtado, Danielle N.; Todorov, Svetoslav D.; Landgraf, Mariza; Destro, Maria T.; Franco, Bernadette D.G.M..
Título: Bacteriocinogenic Lactococcus lactis subsp. lactis DF04Mi isolated from goat milk: characterization of the bacteriocin
Fonte: Braz. j. microbiol;45(4):1541-1550, Oct.-Dec. 2014. tab.
Idioma: en.
Resumo: Lactic acid bacteria capable of producing bacteriocins and presenting probiotic potential open innovative technological applications in the dairy industry. In this study, a bacteriocinogenic strain (Lactococcus lactis subsp. lactis DF4Mi) was isolated from goat milk, and studied for its antimicrobial activity. The bacteriocin presented a broad spectrum of activity, was sensitive to proteolytic enzymes, resistant to heat and pH extremes, and not affected by the presence of SDS, Tween 20, Tween 80, EDTA or NaCl. Bacteriocin production was dependent on the components of the culture media, especially nitrogen source and salts. When tested by PCR, the bacteriocin gene presented 100% homology to nisin Z gene. These properties indicate that this L. lactis subsp. lactis DF4Mi can be used for enhancement of dairy foods safety and quality.
Descritores: Antibacterianos/metabolismo
Bacteriocinas/metabolismo
Lactococcus lactis/metabolismo
Leite/microbiologia
-Sequência de Aminoácidos
Antibacterianos/química
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Bacteriocinas/química
Bacteriocinas/genética
Meios de Cultura/química
Detergentes
DNA Bacteriano/genética
Cabras
Concentração de Íons de Hidrogênio
Lactococcus lactis/crescimento & desenvolvimento
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
Estabilidade Proteica
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Cloreto de Sódio/metabolismo
Temperatura
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde