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Id: biblio-950791
Autor: Shen, Wenlong; Wang, Dong; Ye, Bingyu; Shi, Minglei; Zhang, Yan; Zhao, Zhihu.
Título: A possible role of Drosophila CTCF in mitotic bookmarking and maintaining chromatin domains during the cell cycle
Fonte: Biol. Res;48:1-8, 2015. graf.
Idioma: en.
Projeto: National Basic Research Program of China; . National Natural Science Foundation of China; . National Key Technology R& D Program of China.
Resumo: BACKGROUND: The CCCTC-binding factor (CTCF) is a highly conserved insulator protein that plays various roles in many cellular processes. CTCF is one of the main architecture proteins in higher eukaryotes, and in combination with other architecture proteins and regulators, also shapes the three-dimensional organization of a genome. Experiments show CTCF partially remains associated with chromatin during mitosis. However, the role of CTCF in the maintenance and propagation of genome architectures throughout the cell cycle remains elusive. RESULTS: We performed a comprehensive bioinformatics analysis on public datasets of Drosophila CTCF (dCTCF). We characterized dCTCF-binding sites according to their occupancy status during the cell cycle, and identified three classes: interphase-mitosis-common (IM), interphase-only (IO) and mitosis-only (MO) sites. Integrated function analysis showed dCTCF-binding sites of different classes might be involved in different biological processes, and IM sites were more conserved and more intensely bound. dCTCF-binding sites of the same class preferentially localized closer to each other, and were highly enriched at chromatin syntenic and topologically associating domains boundaries. CONCLUSIONS: Our results revealed different functions of dCTCF during the cell cycle and suggested that dCTCF might contribute to the establishment of the three-dimensional architecture of the Drosophila genome by maintaining local chromatin compartments throughout the whole cell cycle.
Descritores: Proteínas Repressoras/fisiologia
Proteínas de Drosophila/fisiologia
Drosophila melanogaster/química
Genoma de Inseto/genética
-Sítios de Ligação
Sequência de Bases
Ciclo Celular/fisiologia
Sequência Conservada
Biologia Computacional
Montagem e Desmontagem da Cromatina/fisiologia
Anotação de Sequência Molecular
Conjuntos de Dados como Assunto
Fator de Ligação a CCCTC
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central

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Id: lil-750247
Autor: Cambuy, Diego Duque.
Título: Epidemiologia genômica de Bordetella pertussis no Brasil / Genomic epidemiology of bordetella pertussis in Brazil.
Fonte: Rio de Janeiro; s.n; 2014. xiii,85 p. ilus, graf, tab, mapas.
Idioma: pt.
Tese: Apresentada a Instituto Oswaldo Cruz para obtenção do grau de Mestre.
Resumo: A coqueluche, ou pertússis, é uma doença do trato respiratório causada principalmente pela bactéria Bordetella pertussis. Após 50 anos de vacinação, pertussis reemergiu, passando a ser a doença imunoprevinível mais frequente mesmo em países desenvolvidos. Várias são as hipóteses para a reemergência de pertússis, uma delas é a adaptação do patógeno frente à vacinação. Linhagens contemporâneas de B. pertussis diferem de linhagens do período pré-vacinal, especialmente em genes codificadores de proteínas usadas na produção de vacinas acelular. Esta re-emergência também tem sido observada no Brasil, assim, realizamos a caracterização genética por MLST baseado nesses genes, de 26 isolados B. pertussis de surtos de três regiões brasileiras (Norte, Sul e Nordeste). Foram identificados dois perfis alélicos, em 24 isolados: prn2-ptxS1A-fim3B-ptxP3, de surtos (2008-2013) de Alagoas, Pernambuco e Rio Grande do Sul - e o perfil prn2-ptxS1A-fim3A-ptxP3 , em dois isolados de Pará/2004. Análises filogenéticas agruparam esses perfis com isolados do período pós vacinal de outras partes do globo. Deste conjunto, três do perfil mais frequente e um do perfil menos frequente, tiveram seus genomas sequenciados na plataforma GS 454 Junior. A comparação desses genomas com outros genomas de B. pertussis disponíveis em dados públicos não identificou SNPs ou genes únicos que caracterizassem os isolados do Brasil. Este estudo desenvolveu uma metodologia que permitiu definir a posição da IS481 nos genomas, e uma delas corresponde a um gene relacionado a regulação da transcrição da família MarR, Análise filogenômica, baseada em 826 SNPs, demonstrou que os isolados recentes do Brasil da linhagem pandêmica que presente em todos os continentes, exceto a África...

Pertussis more commonly referred as whooping cough is respiratory tract diseasemainly caused by the bacteria B. pertussis. After 50 years of vaccination pertussisremerged, becoming the most frequent vaccine preventable disease in developedcountries. Many hypotheses have been proposed for the re-emergence of pertussis,one being the pathogen adaptation in a vaccinated environment. Current pertussisstrains are different than those from the prevaccination era, especially in genes thatcode for proteins used in acelluar pertussis vaccines. This re-emergence is alsoobserved in Brazil, therefore we characterized 26 isolates from 3 regions of Brazil(North,South,Northeast) using an MLST approach based on these genes. We identifiedtwo allelic profiles, 24 isolates from the states of Rio Grande do Sul (2008-2009),Alagoas (2008-2009), Pernambuco (2013) and Pará (2004) presented the prn2-ptxS1A-fim3B-ptxP3 allelic profile, while 2 isolates from Pará (2004) presented theprn2-ptxS1A-fim3A-ptxP3 allelic profile. Phylogenetic analysis branch these two allelicprofiles along with other post vaccination isolates around the globe. Four isolates, threefrom the dominant profile and one from the less frequent profile, had their genomescompleted sequenced on the GS 454 Junior Platform. We compared these genomeswith others available in public databases and no SNP or unique genes were identifiedin the Brazilian genomes. This study also developed a methodology that identifies thelocation of the repetitive region IS481, and what genes it interrupted. One of them wasthe MarR transcriptional regulator gene. Phylogenomic analysis based on 826 SNPsrevealed that Brazilian B. pertussis lineages are part of the current pandemic linagepresent in all continents, except Africa...
Descritores: Bordetella pertussis/genética
Bordetella pertussis/virologia
Vacinas contra Difteria, Tétano e Coqueluche Acelular
Limites: Humanos
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas

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Id: lil-482044
Autor: Campos, S. R; Rieger, T. T; Santos, J. F.
Título: Homology of polytene elements between Drosophila and Zaprionus determined by in situ hybridization in Zaprionus indianus
Fonte: Genet. mol. res. (Online);6(2):262-276, 2007. ilus, tab.
Idioma: en.
Resumo: The drosophilid Zaprionus indianus due to its economical importance as an insect pest in Brazil deserves more investigation into its genetics. Its mitotic karyotype and a line-drawing map of its polytene chromosomes are already available. This paper presents a photomap of Z. indianus polytene chromosomes, which was used as the reference map for identification of sections marked by in situ hybridization with gene probes. Hybridization signals for Hsp70 and Hsr-omega were detected, respectively, in sections 34B and 32C of chromosome V of Z. indianus, which indicates its homology to the chromosomal arm 3R of Drosophila melanogaster and, therefore, to Muller's element E. The main signal for Hsp83 gene probe hybridization was in section 17C of Z. indianus chromosome III, suggesting its homology to arm 3L of D. melanogaster and to element D of Muller. The Ubi probe hybridized in sections 10C of chromosome II and 17A of chromosome III. Probably the 17A is the polyubiquitin locus, with homology to arm 3L of D. melanogaster and to the mullerian D element, as suggested also by Hsp83 gene location. The Br-C gene was mapped in section 1D, near the tip of the X chromosome, indicating its homology to the X chromosome of D. melanogaster and to mullerian element A. The Dpp gene probe hybridized mainly in the section 32A of chromosome V and, at lower frequencies to other sections, although no signal was observed as expected in the correspondent mullerian B element. This result led to the suggestion of a rearrangement including the Dpp locus in Z. indianus, the secondary signals possibly pointing to related genes of the TGF-beta family. In conclusion, the results indicate that chromosomes X, III, V of Z. indianus are respectively correspondents to elements A, D, and E of Muller. At least chromosome V of Z. indianus seems to share synteny with the 3R arm of D. melanogaster, as indicated by the relative positions of Hsp70 and Hsr-omega, although the Dpp gene indicates a disruption of synteny in its distal region.
Descritores: Cromossomos
Mapeamento Cromossômico
Genes de Insetos
Hibridização In Situ
Limites: Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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