Base de dados : LILACS
Pesquisa : G02.300 [Categoria DeCS]
Referências encontradas : 1009 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 101 ir para página                         

  1 / 1009 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1038257
Autor: Gonçalves, Giovana M; Brianezi, Gabrielli; Miot, Hélio Amante.
Título: The pH of the main Brazilian commercial moisturizers and liquid soaps: considerations on the repair of the skin barrier
Fonte: An. bras. dermatol;92(5):736-738, Sept.-Oct. 2017. tab.
Idioma: en.
Resumo: Abstract: The pH of the skin is slightly acidic (4.6 to 5.8) which is important for appropriate antibacterial, antifungal, constitution of barrier function, as well as structuring and maturation of the stratum corneum. This study aimed to evaluate the pH of the main commercial moisturizers and liquid soaps in Brazil. Thus, pH of the products was quantified by pH meter in three measurements. A total of 38 moisturizers and six commercial liquid soaps were evaluated. Mean pH of 63% and 50% of the moisturizing and liquid soaps presented results above 5.5, disfavoring repair, function, and synthesis of dermal barrier.
Descritores: Sabões/química
Epiderme/efeitos dos fármacos
Concentração de Íons de Hidrogênio
-Brasil
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  2 / 1009 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951810
Autor: Rungrattanakasin, Budsayachat; Premjet, Siripong; Thanonkeo, Sudarat; Klanrit, Preekamol; Thanonkeo, Pornthap.
Título: Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis
Fonte: Braz. j. microbiol;49(3):647-655, July-Sept. 2018. graf.
Idioma: en.
Resumo: Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and encoded a protein of 460 amino acid residues. The predicted molecular weight and the isoelectric point of the A. fumigatus Cel7 gene product were 48.19 kDa and 5.03, respectively. A catalytic domain in the N-terminal region and a fungal type cellulose-binding domain/module in the C-terminal region were detected in the predicted polypeptide sequences. Furthermore, a signal peptide with 20 amino acid residues at the N-terminus was also detected in the deduced amino acid sequences of the endoglucanase from A. fumigatus DBiNU-1. The endoglucanase from A. fumigatus DBiNU-1 was successfully expressed in K. lactis, and the purified recombinant enzyme exhibited its maximum activity at pH 5.0 and 60 °C. The enzyme was very stable in a pH range from 4.0 to 8.0 and a temperature range from 30 to 60 °C. These features make it suitable for application in the paper, biofuel, and other chemical production industries that use cellulosic materials.
Descritores: Aspergillus fumigatus/enzimologia
Proteínas Fúngicas/genética
Proteínas Fúngicas/química
Expressão Gênica
Celulase/genética
Celulase/química
Clonagem Molecular
-Aspergillus fumigatus/genética
Especificidade por Substrato
Estabilidade Enzimática
Kluyveromyces/genética
Kluyveromyces/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/química
Proteínas Fúngicas/metabolismo
Celulase/metabolismo
Temperatura Alta
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME


  3 / 1009 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974326
Autor: Fraga, Martín; Fernández, Sofía; Perelmuter, Karen; Pomiés, Nicolle; Cajarville, Cecilia; Zunino, Pablo.
Título: The use of Prevotella bryantii 3C5 for modulation of the ruminal environment in an ovine model
Fonte: Braz. j. microbiol;49(supl.1):101-106, 2018. tab, graf.
Idioma: en.
Projeto: Agencia Nacional de Investigación.
Resumo: Abstract In the Southern Hemisphere, ruminants are mostly raised in grazing systems where animals consume forage and are supplemented with low amounts of concentrates. Concentrates are usually given separately and are rapidly ingested. This practice leads to changing rumen environment conditions during the day, may alter the rumen microbial metabolism and could affect host performance. The native ruminal Prevotella bryantii strain 3C5 was administered every 48 h to wethers under experimental conditions simulating Southern-Hemisphere feeding to evaluate its potential as a rumen fermentation modulator. The inoculum potential was assessed on day 17. The ammonia nitrogen (NH3-N), volatile fatty acids and ruminal pH were monitored on a 24-h basis 19 days after the beginning of the experiment, and the microbial community structure was assessed by pyrosequencing. The administration of P. bryantii modified the fermentation products and daily pH values compared to the control. The NH3-N concentration in the rumen of treated animals was significantly higher than that of the untreated animals. Modification of the ruminal environment and fermentation pathways was achieved without altering the general structure of the microbial community or the potential methane production. P. bryantii 3C5 could be considered in potential probiotic formulations for ruminants in semi-intensive systems.
Descritores: Rúmen/microbiologia
Prevotella/metabolismo
-Rúmen/metabolismo
Rúmen/química
Ovinos
Prevotella/genética
Digestão
Ácidos Graxos Voláteis/metabolismo
Fermentação
Amônia/metabolismo
Concentração de Íons de Hidrogênio
Modelos Biológicos
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


  4 / 1009 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974320
Autor: Yuwa-amornpitak, Thalisa; Chookietwatana, Kannika.
Título: Bioconversion of waste cooking oil glycerol from cabbage extract to lactic acid by Rhizopus microsporus
Fonte: Braz. j. microbiol;49(supl.1):178-184, 2018. tab, graf.
Idioma: en.
Projeto: Mahasarakham University.
Resumo: Abstract Glycerol from spent oil was processed by transesterification for biodiesel production. Although glycerol contains many types of impurities, it can be used as a C-source for lactic acid production by fungi, such as Rhizopus microsporus. In this study, we found that wild type R. microsporus (LTH23) produced more lactic acid than the mutant strains on cabbage glycerol media (CG media). More lactic acid was produced on CG media than on cabbage extract media (C media) by about two-fold in batch fermentation conditions. In addition, we found that lactic acid production in a fed-batch process was also slightly higher than in a batch process. To study the combined effects of pH, urea, and glycerol waste concentration on lactic acid production, a response surface methodology was used. The optimum pH, urea, and glycerol waste concentrations were pH 6.5, 3.75 g/L, and 17 g/L, respectively. The maximum lactic acid production predicted by this equation model was 4.03 g/L.
Descritores: Rhizopus/metabolismo
Brassica/química
Ácido Láctico/metabolismo
Glicerol/metabolismo
-Resíduos/análise
Brassica/metabolismo
Brassica/microbiologia
Biotransformação
Culinária
Meios de Cultura/metabolismo
Meios de Cultura/química
Biocombustíveis/análise
Fermentação
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME


  5 / 1009 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974316
Autor: Reis, Cristiane Bianchi Loureiro dos; Morandini, Liziane Maria Barassuol; Bevilacqua, Caroline Borges; Bublitz, Fabricio; Ugalde, Gustavo; Mazutti, Marcio Antonio; Jacques, Rodrigo Josemar Seminoti.
Título: First report of the production of a potent biosurfactant with α, ß-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation
Fonte: Braz. j. microbiol;49(supl.1):185-192, 2018. tab, graf.
Idioma: en.
Resumo: Abstract Biosurfactants have many advantages over synthetic surfactants but have higher production costs. Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost. The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule. The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi. To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used. The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose. The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry. F. fujikuroi reduced surface tension from 72 to 20 mN m−1 under the optimized conditions of pH 5.0, 37 °C and 7 days of incubation with 190 rpm agitation. The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose. The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.
Descritores: Tensoativos/metabolismo
Trealose/metabolismo
Microbiologia Industrial/métodos
Fusarium/metabolismo
-Tensoativos/química
Temperatura Ambiente
Meios de Cultura/metabolismo
Fermentação
Fusarium/crescimento & desenvolvimento
Fusarium/química
Concentração de Íons de Hidrogênio
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


  6 / 1009 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-828208
Autor: Munaganti, Rajesh Kumar; Muvva, Vijayalakshmi; Konda, Saidulu; Naragani, Krishna; Mangamuri, Usha Kiranmayi; Dorigondla, Kumar Reddy; Akkewar, Dattatray. M.
Título: Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards
Fonte: Braz. j. microbiol;47(4):1030-1038, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract An actinobacterial strain VL-RK_09 having potential antimicrobial activities was isolated from a mango orchard in Krishna District, Andhra Pradesh (India) and was identified as Arthrobacter kerguelensis. The strain A. kerguelensis VL-RK_09 exhibited a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. Production of bioactive metabolites by the strain was the highest in modified yeast extract malt extract dextrose broth, as compared to other media tested. Lactose (1%) and peptone (0.5%) were found to be the most suitable carbon and nitrogen sources, respectively, for the optimum production of the bioactive metabolites. The maximum production of the bioactive metabolites was detected in the culture medium with an initial pH of 7, in which the strain was incubated for five days at 30 °C under shaking conditions. Screening of secondary metabolites obtained from the culture broth led to the isolation of a compound active against a wide variety of Gram-positive and negative bacteria and fungi. The structure of the first active fraction was elucidated using Fourier transform infrared spectroscopy, electrospray ionization mass spectrometry, 1H and 13C nuclear magnetic resonance spectroscopy. The compound was identified as S,S-dipropyl carbonodithioate. This study is the first report of the occurrence of this compound in the genus Arthrobacter.
Descritores: Arthrobacter/isolamento & purificação
Arthrobacter/metabolismo
Mangifera/microbiologia
Anti-Infecciosos/metabolismo
Anti-Infecciosos/farmacologia
-Sais/metabolismo
Temperatura Ambiente
Carbono/metabolismo
Testes de Sensibilidade Microbiana
Metaboloma
Metabolômica/métodos
Concentração de Íons de Hidrogênio
Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/química
Minerais/metabolismo
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  7 / 1009 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-828205
Autor: Aliabadi, Nasrin; Aminzadeh, Saeed; Karkhane, Ali Asghar; Haghbeen, Kamahldin.
Título: Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
Fonte: Braz. j. microbiol;47(4):931-940, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: International Foundation for Science; . Committee on Scientific and Technological Cooperation; . National Institute of Genetic Engineering and Biotechnology.
Resumo: Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and temperature of incubation were the most significant factors influencing chitinase production. From the tested values, the best pH/temperature was obtained at pH 5 and 70 °C, with Km and V max values of chitinase to be 5.6 mg/mL and 0.87 µmol/min, respectively. Ag+, Co2+, iodoacetamide, and iodoacetic acid inhibited the enzyme activity, whereas Mn2+, Cu2+, Tweens (20 and 80), Triton X-100, and EDTA increased the same. In addition, the study of the morphological alteration of chitin treated by enzyme by SEM revealed cracks and pores on the chitin surface, indicating a potential application of this enzyme in several industries.
Descritores: Bacillus/metabolismo
Quitinases/metabolismo
-Fósforo/metabolismo
Temperatura Ambiente
Bacillus/isolamento & purificação
Bacillus/genética
Bacillus/ultraestrutura
Estabilidade Enzimática/efeitos dos fármacos
Carbono/metabolismo
RNA Ribossômico 16S/genética
Cinética
Quitinases/química
Análise de Sequência de DNA
Ativação Enzimática
Concentração de Íons de Hidrogênio
Íons
Metais
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  8 / 1009 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-828202
Autor: Jha, Sujata S; Joshi, Sanket J; S. J., Geetha.
Título: Lipopeptide production by Bacillus subtilis R1 and its possible applications
Fonte: Braz. j. microbiol;47(4):955-964, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract The possible application of a bacterial strain - Bacillus subtilis R1, isolated from an oil contaminated desert site in India, as biocontrol agent and its biosurfactant in microbial enhanced oil recovery are discussed. The biosurfactant production in minimal medium was carried out at different temperatures and salt concentrations, where it produced an efficient biosurfactant at 30-45 °C and in presence of up to 7% salt. It significantly reduced the surface tension from 66 ± 1.25 mN/m to 29 ± 0.85 mN/m within 24 h. In order to enhance the biosurfactant production, random mutagenesis of B. subtilis R1 was performed using chemical mutagen - ethyl methanesulfonate. Majority of the isolated 42 mutants showed biosurfactant production, but the difference was statistically insignificant as compared with parent strain R1. Therefore none of the mutants were selected for further study, and only parent strain R1 was studied. The biosurfactant was quite stable under harsh conditions for up to 10 days. The biosurfactant was extracted and characterized as similar to the lipopeptide group - surfactins and fengycin. The crude oil displacement experiments using biosurfactant broth in sand pack glass columns showed 33 ± 1.25% additional oil recovery. The strain also showed inhibition of various plant pathogenic fungi on potato dextrose agar medium.
Descritores: Bacillus subtilis/metabolismo
Lipopeptídeos/biossíntese
-Tensoativos/metabolismo
Tensoativos/farmacologia
Bacillus subtilis/classificação
Bacillus subtilis/genética
RNA Ribossômico 16S/genética
Testes de Sensibilidade Microbiana
Mutagênese
Espectroscopia de Infravermelho com Transformada de Fourier
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Lipopeptídeos/farmacologia
Engenharia Metabólica
Concentração de Íons de Hidrogênio
Antifúngicos/metabolismo
Antifúngicos/farmacologia
Responsável: BR1.1 - BIREME


  9 / 1009 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-788980
Autor: El-Gendy, Mervat Morsy Abbas Ahmed; El-Bondkly, Ahmed Mohamed Ahmed.
Título: Evaluation and enhancement of heavy metals bioremediation in aqueous solutions by Nocardiopsis sp. MORSY1948, and Nocardia sp. MORSY2014
Fonte: Braz. j. microbiol;47(3):571-586, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT An analysis of wastewater samples collected from different industrial regions of Egypt demonstrated dangerously high levels of nickel (0.27-31.50 mg L-1), chromium (1.50-7.41 mg L-1) and zinc (1.91-9.74 mg L-1) in the effluents. Alarmingly, these heavy metals are among the most toxic knownones to humans and wildlife. Sixty-nine Actinomycete isolates derived from contaminated sites were evaluated under single, binary, and ternary systems for their biosorption capacity for Ni2+, Cr6+ and Zn2+ from aqueous solutions. The results of the study identified isolates MORSY1948 and MORSY2014 as the most active biosorbents. Phenotypic and chemotypic characterization along with molecular phylogenetic evidence confirmed that the two strains are members of the Nocardiopsis and Nocardia genera, respectively. The results also proved that for both the strains, heavy metal reduction was more efficient with dead rather than live biomass. The affinity of the dead biomass of MORSY1948 strain for Ni2+, Cr6+ and Zn2+ under the optimized pH conditions of 7, 8 and 7, respectively at 40 °C temperature with 0.3% biosorbent dosage was found to be as follows: Ni2+ (87.90%) > Zn2+ (84.15%) > Cr6+ (63.75%). However, the dead biomass of MORSY2014 strain under conditions of pH 8 and 50 °C temperature with 0.3% biosorbent dose exhibited the highest affinity which was as follows: Cr6+ (95.22%) > Ni2+ (93.53%) > Zn2+ (90.37%). All heavy metals under study were found to be removed from aqueous solutions in entirety when the sorbent dosage was increased to 0.4%.
Descritores: Metais Pesados/metabolismo
Nocardia/classificação
Nocardia/metabolismo
-Temperatura Ambiente
Fatores de Tempo
Poluentes Químicos da Água/metabolismo
Biodegradação Ambiental
RNA Ribossômico 16S/genética
Biomassa
Metais Pesados/toxicidade
Adsorção
Egito
Águas Residuais/microbiologia
Concentração de Íons de Hidrogênio
Resíduos Industriais
Nocardia/isolamento & purificação
Nocardia/genética
Responsável: BR1.1 - BIREME


  10 / 1009 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-788974
Autor: Gururaj, P; Ramalingam, Subramanian; Devi, Ganesan Nandhini; Gautam, Pennathur.
Título: Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
Fonte: Braz. j. microbiol;47(3):647-657, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: Department of Biotechnology, Government of India.
Resumo: ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a 0.5% (v/v) inoculum, 2% (v/v) castor oil (inducer), and agitation 150 rpm. The optimized conditions from the shake flask experiments were validated in a 3 L lab scale bioreactor, and the lipase production increased to 48 U/mL. The enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography and the overall yield was 36%. SDS-PAGE indicated a molecular weight of 45 kDa for the purified protein, and Matrix assisted laser desorption/ionization time of flight analysis of the purified lipase showed sequence similarity with GDSL family of lipases. The optimum temperature and pH for activity of the enzyme was found to be 50 ºC and 8.0, respectively. The lipase was completely inhibited by phenylmethylsulfonyl fluoride but minimal inhibition was observed when incubated with ethylenediaminetetraacetic acid and dithiothreitol. The enzyme was stable in the presence of non-polar hydrophobic solvents. Detergents like SDS inhibited enzyme activity; however, there was minimal loss of enzyme activity when incubated with hydrogen peroxide, Tween 80 and Triton X-100. The kinetic constants (Km and Vmax) revealed that the hydrolytic activity of the lipase was specific to moderate chain fatty acid esters. The Vmax, Km and Vmax/Km ratio of the enzyme were 16.98 U/mg, 0.51 mM, and 33.29, respectively when 4-nitrophenyl palmitate was used as a substrate.
Descritores: Compostos Orgânicos
Solventes
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/biossíntese
Acinetobacter/enzimologia
Lipase/isolamento & purificação
Lipase/biossíntese
-Compostos Orgânicos/química
Solventes/química
Especificidade por Substrato
Temperatura Ambiente
Proteínas de Bactérias/química
Estabilidade Enzimática
Cinética
Cromatografia por Troca Iônica
Ativação Enzimática
Espaço Extracelular/enzimologia
Concentração de Íons de Hidrogênio
Íons
Lipase/química
Lipólise
Metais
Peso Molecular
Responsável: BR1.1 - BIREME



página 1 de 101 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde