Base de dados : LILACS
Pesquisa : G02.700 [Categoria DeCS]
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Id: biblio-843175
Autor: Coletti Zabala, Tamara L; Zerbatto, María E; Perotti, Elda B. R; Smacchia, Ana M; Ombrella, Adriana; Pidello, Alejandro R.
Título: Efecto del metronidazol suplementado con hidroquinona en la adherencia de Lactobacillus acidophilus en células vaginales ovinas / Effect of metronidazole supplemented with hydroquinone on the adhesion of Lactobacillus acidophilus in ovine vaginal cells
Fonte: Rev. argent. microbiol;48(4):313-319, dic. 2016. ilus, graf, tab.
Idioma: en.
Resumo: This work demonstrates that the addition of metronidazole together with a ubiquitous quinone compound reduces adherence of Lactobacillus acidophilus to ovine vaginal cells. Spectrophotometric and voltammetric studies have shown that neoformed compounds were observed in these systems; there were also changes in their electroactive composition, and the oxidant status had a significantly higher value compared to the control (p < 0.05). Based on reduction potential (E; mV), the distribution of electroactive compound concentrations suggests that the compounds with low reduction potential induce this behavior, which would indicate that the addition of metronidazole with a ubiquitous quinone compound to the vaginal system might increase the reductive capacity of these systems. This work shows that the study of behavior and fluctuations of the redox compounds that compose the vaginal environment, in terms of concentration and species of redox molecules, must be hierarchized in order to better understand the early stages of colonization by microorganisms.

Este trabajo demuestra que la incorporación de metronidazol conjuntamente con un compuesto quinónico ubicuo disminuye la adherencia de Lactobacillus acidophilus a células vaginales ovinas. Los estudios espectrofotométricos y voltamétricos mostraron que en estos sistemas aparecieron compuestos neoformados y que hubo modificaciones en la composición electroactiva; asimismo, el estatus oxidante presentó un valor significativamente superior con respecto al control (p < 0,05). Según los potenciales de reducción (E; mV), la distribución de las concentraciones de los compuestos electroactivos muestra que los compuestos con bajos potenciales de reducción inducen este comportamiento. Esto indicaría que la incorporación de esta mezcla al sistema vaginal aumentaría su capacidad reductora. El trabajo muestra que el estudio del comportamiento y las fluctuaciones de los compuestos redox que componen el ambiente vaginal, en términos de concentración y especies moleculares, debe ser jerarquizado para comprender mejor las primeras etapas de la colonización de este ambiente por parte de los microorganismos.
Descritores: Doenças dos Ovinos/prevenção & controle
Doenças Vaginais/veterinária
Aderência Bacteriana/efeitos dos fármacos
Metronidazol/análise
-Oxirredução/efeitos dos fármacos
Espectrofotometria/métodos
Ovinos/microbiologia
Metronidazol/farmacologia
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Estudo Observacional Veterinário
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-906720
Autor: Fernandes, Carolina Gonçalves; Laurindo, Francisco Rafael Martins.
Título: Estresse oxidativo como mecanismo comum a várias doenças vasculares: uma análise crítica / Oxidative stress as a mechanism common to several cardiovascular diseases: a critical analysis
Fonte: Rev. Soc. Cardiol. Estado de Säo Paulo;28(1), jan.-mar. 2018. ilus.
Idioma: pt.
Resumo: Estresse oxidativo é um conceito em evolução. Muito mais do que um simples desbalanço entre oxidantes e antioxidantes, representa um desequilíbrio de vias de sinalização celular redox. Sinalização redox envolve a produção de espécies reativas de oxigênio, radicalares e não radicalares, assim como vias não radicalares de oxidação por dois elétrons. Está claro que essas espécies podem ser produzidas por mecanismos enzimáticos ancestrais e ubíquos e, portanto, não representam acidentes, tendo vários efeitos fisiológicos benéficos. Novas vias de sinalização redox têm sido descritas, com ênfase no papel de peroxiredoxinas, outras tiolproteínas e estados intermediários de oxidação de tióis. Estes fatos levaram à necessidade de reavaliar a definição e a abrangência do conceito de antioxidantes. Apesar da ineficácia dos estudos clínicos com antioxidantes clássicos, há novas perspectivas, que incluem inibidores de enzimas, geradores de geram oxidantes, como NADPH oxidases e vias mitocondriais. Além disso, o uso de flavonóides e compostos relacionados, capazes de ativar vias horméticas de proteção antioxidante, é um caminho importante. Esses fatos indicam que a ciência da área deve entrar em um novo ciclo de estudos para testar novas intervenções clínicas resultantes desses avanços

Oxidative stress is an evolving concept. Much more than a simple imbalance between oxidants and antioxidants, it represents an imbalance of the cellular redox signaling pathways. Redox signaling involves the production of reactive oxygen species, both radicular and non-radicular, as well as non-radicular two-electron oxidation pathways. It is clear that these species can be produced by ancestral and ubiquitous enzymatic mechanisms, therefore they do not represent accidents, and have various beneficial physiological effects. New redox signaling pathways have been described, with emphasis on the role of peroxiredoxins, other protein thiols, and intermediary states of thiol oxidation. These facts have created a need to reassess the definition and scope of the concept of antioxidants. Despite the inefficiency of clinical studies with classic antioxidants, there are new perspectives, which include oxidant-producing enzyme inhibitors, such as NADPH oxidases and mitochondrial pathways. Furthermore, the use of flavonoids and related compounds, capable of activating hormetic pathways of antioxidant protection, is an important route. These facts indicate that the science in the area should enter a new cycle of studies, testing new clinical interventions resulting from these advances
Descritores: Endotélio Vascular
Doenças Cardiovasculares/fisiopatologia
Estresse Oxidativo/fisiologia
-Oxirredução
Flavonoides
Enzimas
Aterosclerose
Inflamação
Antioxidantes
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: BR44.1 - Serviço de Biblioteca, Documentação Científica e Didática Prof. Dr. Luiz Venere Décourt


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Id: biblio-1045580
Autor: Alleyne, T; Ashe, D.
Título: Hydrogen/rydride ion relay - a mechanism for early electron transfer in cytochrome c oxidases / El reléuónico hidrógeno-hidruro: un mecanismo de transferencia temprana de electrones en las citocromo c oxidasas
Fonte: West Indian med. j;62(1):3-11, Jan. 2013. ilus, tab.
Idioma: en.
Resumo: Cytochrome c oxidase (COX) employs electrons obtained from cytochrome c to bring about the reduction of oxygen to water. It is known that the electrons originate from the haem edge of cytochrome c and enters bovine COX at Trp-104. It is also known that Tyr-105, Glu-198 and Asp-158 of COX subunit II play roles in the enzyme's catalysis but how these roles are linked to electron transfer remain unclear. Recently, we proposed that electrons travel from the haem edge of cytochrome c to CuA, the first metal redox centre of COX, by a hydrogen/hydride ion relay using six residues. Now using a similar computer assisted approach, we investigate the extent to which this hydride/hydrogen ion mechanism is common amongst oxidases. The crystal structures of COX from P denitrificans, R sphaeroides and T thermophilus and quinol oxidase from E coli were downloaded and their binding domains analysed. As with bovine, all four oxidases had only nine amino acid residues in that region and both the sequences and three-dimensional structures were highly conserved. We propose that these residues function as a hydrogen/hydride ion relay, participating directly in electron transfer to CuA. We further suggest that this electron transfer mechanism might be a common feature in oxidases.

La citocromo c oxidasa (COX) emplea electrones obtenidos del citocromo c para producir la reducción del oxígeno a agua. Se sabe que los electrones originan a partir del hemo del citocromo c, y entran en la COX bovina en Trp-104. También se conoce que Tyr-105, Glu-198 y Asp-158 de la subunidad II de COX, desempeñan papeles en la catálisis de la enzima, pero no hay todavía claridad en cuanto a cómo estos papeles se hallan vinculados con la transferencia de electrones. Recientemente, sugerimos que los electrones viajan del borde del hemo del citocromo c al CuA, el primer centro metálico de reacción redox de la COX, por un relé iónico hidrógeno-hidruro, usando seis residuos. Ahora, usando un enfoque similar computarizado, investigamos hasta que punto este mecanismo de iones hidrógeno/hidruro es común entre las oxidasas. Se bajaron y analizaron los dominios de unión de las estructuras cristalinas de la COX de P denitrificans, R sphaeroides, y T thermophilus, y de la quinol oxidasa de la E coli. Como en el caso de la bovina, las cuatro oxidasas tenían sólo nueve residuos de aminoácido en esa región, y tanto las secuencias como las estructuras tridimensionales presentaban un alto grado de conservación. Proponemos que estos residuos funcionan como un relé iónico hidrógeno-hidruro, participando directamente en una transferencia de electrones al CuA. Asimismo, sugerimos que este mecanismo de transferencia de electrones podría ser un rasgo común de las oxidasas.
Descritores: Complexo IV da Cadeia de Transporte de Elétrons/metabolismo
Citocromos c/metabolismo
Heme/química
Hidrogênio/metabolismo
-Oxirredução
Paracoccus denitrificans/enzimologia
Prótons
Rhodobacter sphaeroides/enzimologia
Sequência de Aminoácidos
Thermus thermophilus/enzimologia
Escherichia coli/enzimologia
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


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Id: biblio-974905
Autor: Low, Sandra Trindade; Ferraz, Ana Paula Costa Rodrigues; Maio, Regiane; Francisqueti, Fabiane Valentini; Pierine, Damiana Tortolero; Borges, Weidylla Natália Silva; Carvalho, Ariel Dandara de; Lima, Gedalva Pereira de; Ferreira, Ana Lúcia A; dos Santos, Klinsmann Carolo; Corrêa, Camila Renata.
Título: Factors that influence the redox state in children: An exploratory study
Fonte: Clinics;73:e335, 2018. tab.
Idioma: en.
Projeto: Processo AUX-PE-DINTER.
Resumo: OBJECTIVE: The aim of the present study was to investigate the association of the redox state via malondialdehyde (MDA) as a lipid peroxidation biomarker and hydrophilic antioxidant capacity (HAC) with dietary, anthropometric, demographic, socio-economic and clinical variables as well as the serum concentrations of vitamins in children aged 20-36 months. This cross-sectional study was conducted from May 2013 to May 2014 and included a total of 100 children. METHODS: The variables studied included anthropometric measurements, dietary intake by the Food Frequency Questionnaire (FFQ), socio-demographic features, clinical attributes, serum redox status, and serum vitamin concentrations. RESULTS: Children with a family income above the minimum wage and adequate body mass index (BMI) presented higher HAC. The MDA concentration was higher in children older than 24 months. Breastfeeding for up to 120 days provided greater antioxidant capacity. Children classified in the 2nd tertile for "fruit and vegetables" and "milk and dairy products" consumption showed lower levels of MDA. There was a positive correlation of MDA with serum vitamin A levels. These results show that among children in the 20-36 months age group, family income, breastfeeding, BMI and intake of fruits and vegetables can have an influence on the imbalance of the redox state. CONCLUSION: One strategy to prevent the imbalance between oxidants and antioxidants could be for health professionals to raise awareness among families, as such knowledge could repress/prevent the progression/initiation of several diseases in adult life.
Descritores: Peroxidação de Lipídeos/efeitos dos fármacos
Estado Nutricional/fisiologia
Comportamento Alimentar/fisiologia
Antioxidantes/administração & dosagem
-Oxirredução
Fatores Socioeconômicos
Peroxidação de Lipídeos/fisiologia
Estudos Transversais
Limites: Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Responsável: BR1.1 - BIREME


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Id: biblio-950720
Autor: Chaudhuri, Dipankar; Ghate, Nikhil Baban; Deb, Shampa; Panja, Sourav; Sarkar, Rhitajit; Rout, Jayashree; Mandal, Nripendranath.
Título: Assessment of the phytochemical constituents and antioxidant activity of a bloom forming microalgae Euglena tuba
Fonte: Biol. Res;47:1-11, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Unstable generation of free radicals in the body are responsible for many degenerative diseases. A bloom forming algae Euglena tuba growing abundantly in the aquatic habitats of Cachar district in the state of Assam in North-East India was analysed for its phytochemical contents, antioxidant activity as well as free radical scavenging potentials. RESULTS: Based on the ability of the extract in ABTS•+ radical cation inhibition and Fe3+ reducing power, the obtained results revealed the prominent antioxidant activity of the algae, with high correlation coefficient of its TEAC values to the respective phenolic and flavonoid contents. The extract had shown its scavenging activity for different free radicals and 41.89 ± 0.41 µg/ml, 5.83 ± 0.07 µg/ml, 278.46 ± 15.02 µg/ml and 223.25 ± 4.19 µg/ml were determined as the IC50 values for hydroxyl, superoxide, nitric oxide and hypochlorous acid respectively, which are lower than that of the corresponding reference standards. The phytochemical analysis also revealed that the phenolics, flavonoids, alkaloids, tannins and carbohydrates are present in adequate amount in the extract which was confirmed by HPLC analysis. CONCLUSIONS: The results showed that 70% methanol extract of the algae possesses excellent antioxidant and free radical scavenging properties.
Descritores: Extratos Celulares/química
Sequestradores de Radicais Livres/metabolismo
Substâncias Redutoras/metabolismo
Euglena/química
Antioxidantes/metabolismo
-Oxirredução
Fenóis/análise
Ácido Ascórbico/análise
Taninos/análise
Flavonoides/análise
Peroxidação de Lipídeos/efeitos dos fármacos
Cromanos/metabolismo
Cromatografia Líquida de Alta Pressão
Metanol
Alcaloides/análise
Microalgas
Glucose/análise
Índia
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1134303
Autor: Cicek, Ozge Senem Yucel; Kaya, Gozde; Alyuruk, Begum; Doger, Emek; Girisen, Tugba; Filiz, Serdar.
Título: The association of seminal oxidation reduction potential with sperm parameters in patients with unexplained and male factor infertility
Fonte: Int. braz. j. urol;47(1):112-119, Jan.-Feb. 2021. tab, graf.
Idioma: en.
Resumo: ABSTRACT Purpose: Understanding the effects of high oxidation reduction potential (ORP) levels on sperm parameters will help to identify patients with unexplained and male factor infertility who may have seminal oxidative stress and determine if ORP testing is needed. This study aimed to evaluate the association between seminal ORP and conventional sperm parameters. Materials and Methods: A total of 58 patients who provided a semen sample for simultaneous evaluation of sperm parameters and ORP between January and September 2019 were enrolled in this retrospective study. To identify normal and high ORP levels, a static ORP (sORP) cut-off value of 1.36mV/106sperm/mL was used. Sperm parameters were compared between infertile men with normal sORP (control group, n=23) and high sORP values (study group, n=35). Results: Men with sORP values >1.36mV/106sperm/mL had significantly lower total sperm count (TSC) (p <0.001), sperm concentration (p <0.001) and total motile sperm count (TMSC) (p <0.001). In addition, progressive motility (p=0.04) and fast forward progressive motility (p <0.001) were significantly lower in the study group. A negative correlation was found between sORP and TSC (r=-0.820, p <0.001), sperm concentration (r=-0.822, p <0.001), TMSC (r=-0.808, p <0.001) and progressive motility (r=-0.378, p=0.004). Non-progressive motility positively correlated with sORP (r=0.344, p=0.010). Conclusions: This study has shown that TSC, sperm concentration, progressive motility and TMSC are associated with seminal oxidative stress, indicated by a sORP cut-off of 1.36mV/106sperm/mL. Presence of oligozoospermia, reduced progressive motilty or low TMSC in sperm analysis should raise the suspicion of oxidative stress and warrants seminal ROS testing.
Descritores: Motilidade Espermática
Infertilidade Masculina
-Oxirredução
Sêmen
Contagem de Espermatozoides
Espermatozoides
Estudos Retrospectivos
Limites: Humanos
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-1132180
Autor: Ribeiro, Iára Mariana Léllis; Pereira, Wagner Luiz; Nogueira, Leonardo Brandão; Oliveira, Laser Antônio Machado; Teixeira, Róbson Ricardo; Nogueira, Katiane de Oliveira Pinto Coelho.
Título: Neuroprotective Effect of Isobenzofuranones on Hydrogen Peroxide-Mediated Redox Imbalance in Primary Cultures of Hippocampal Neurons
Fonte: Braz. arch. biol. technol;63:e20190072, 2020. graf.
Idioma: en.
Projeto: Minas Gerais Funding Foundation.
Resumo: Abstract In live organisms, there is a balance between the production of reactive oxygen species (ROS) and their neutralization. The increased level of these species leads to a condition called redox imbalance. The aim of this study was to evaluate the protective action of isobenzofuranones in primary cultures of hippocampal neurons subjected to redox imbalance. To accomplish this, MTT and LIVE/DEAD assays were initially performed. In the cultures pretreated with isobenzofuranones 1 and 2, there was a higher number of live cells when compared to that in the untreated ones. Regarding redox imbalance, there was a significant increase in the intracellular levels of ROS. The cultures pretreated with isobenzofuranones showed a reduction in ROS levels. Lipid peroxidation caused by oxidative damage was significantly reduced in the cultures pretreated with isobenzofuranones 1 and 2. Taken together, these data show the ability of isobenzofuranones 1 and 2 to significantly minimize cytotoxicity, cell death, intracellular levels of ROS and lipid peroxidation induced by redox imbalance. These results suggest that isobenzofuranones 1 and 2 represent a possible alternative therapy for the neurodegenerative disturbances that are triggered by ROS production increases.
Descritores: Oxirredução/efeitos dos fármacos
Benzofuranos/farmacologia
Espécies Reativas de Oxigênio
Fármacos Neuroprotetores/farmacologia
Peróxido de Hidrogênio
-Benzofuranos/síntese química
Morte Celular
Cultura Primária de Células
Hipocampo/citologia
Neurônios/metabolismo
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Castro, M
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Id: biblio-1124211
Autor: Muñoz-Villagrán, C; Contreras, F; Cornejo, F; Figueroa, M; Valenzuela-Bezanilla, D; Luraschi, R; Reinoso, C; Rivas-Pardo, J; Vásquez, C; Castro, M; Arenas, F.
Título: Understanding gold toxicity in aerobically-grown Escherichia coli
Fonte: Biol. Res;53:26, 2020. graf.
Idioma: en.
Projeto: FONDECYT (Fondo Nacional de Ciencia y Tecnología); . DICYT; . USA1799 Vridei (Vicerrectoría de investigación, desarrollo e innovación).
Resumo: BACKGROUND: There Is an emerging field to put Into practice new strategies for developing molecules with antimicrobial properties. In this line, several metals and metalloids are currently being used for these purposes, although their cellular effect(s) or target(s) in a particular organism are still unknown. Here we aimed to investigate and analyze Au3+ toxicity through a combination of biochemical and molecular approaches. RESULTS: We found that Au3+ triggers a major oxidative unbalance in Escherichia coli, characterized by decreased intracellular thiol levels, increased superoxide concentration, as well as by an augmented production of the antioxidant enzymes superoxide dismutase and catalase. Because ROS production is, in some cases, associated with metal reduction and the concomitant generation of gold-containing nanostructures (AuNS), this possibility was evaluated in vivo and in vitro. CONCLUSIONS: Au3+ is toxic for E. coli because it triggers an unbalance of the bacterium's oxidative status. This was demonstrated by using oxidative stress dyes and antioxidant chemicals as well as gene reporters, RSH concentrations and AuNS generation.
Descritores: Oxirredução/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Escherichia coli/efeitos dos fármacos
Nanopartículas Metálicas/toxicidade
Ouro/toxicidade
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1136263
Autor: Alghadir, Ahmad H; Gabr, Sami A.
Título: Physical activity impact on motor development and oxidative stress biomarkers in school children with intellectual disability
Fonte: Rev Assoc Med Bras (1992);66(5):600-606, 2020. tab, graf.
Idioma: en.
Resumo: SUMMARY OBJECTIVE Lower physical fitness and poor motor performance were shown to be linked with higher levels of oxidative stress in children and adolescents with intellectual disabilities. Therefore, a moderate aerobic exercise for 12-weeks was performed to evaluate the effects of physical activity scores on motor functions, oxidative stress, and intelligence quotients (IQ) in school children with intellectual disability. METHODS A total of 65 school children aged (12-18 Yrs) were randomly included in this study. Intellectual disability (ID),motor skills,physical fitness(VO2max), total energy expenditure (TEE), MDA, 8-OHdG, TAC, NO, and total oxidative stress(OS)were assessed using pre-validated WISC-IQ score test, BOT-2 test, PA questionnaire, and immunoassay techniques respectively. RESULTS WISC-IQ and BOT-2 set scores of intellectual and motor skills performance showed a significant correlation with physical activity status and the regulation of oxidative stress-free radicals in school children with mild and moderate ID following 12 weeks of moderate exercise. The intellectual and motor skills performance of the participants correlated positively with the increase in TAC activity and physical fitness scores and negatively with MDA, 8-OHdG, NO, and Total-OS, respectively. Stepwise multiple regression analysis of the demographic, physical status and oxidative stress parameters explained around78.0 to 93.4 % of intellectual disability variation among schoolchildren. CONCLUSION Moderate aerobic training for12 weeks has a positive impact on improving intellectual ability of schoolchildren with ID via modulating redox status, improves physical fitness, and motor skills proficiency.

RESUMO OBJETIVO A baixa aptidão física e o baixo desempenho motor mostraram-se associados a níveis mais altos de estresse oxidativo em crianças e adolescentes com deficiência intelectual. Portanto, foi realizado um exercício aeróbico moderado por 12 semanas para avaliar os efeitos dos escores de atividade física nas funções motoras, estresse oxidativo e quocientes de inteligência (QI) em escolares com deficiência intelectual. MÉTODOS Um total de 65 crianças em idade escolar (12 a 18 anos) foi incluído aleatoriamente neste estudo. A incapacidade intelectual (DI), habilidades motoras, aptidão física (VO2máx), gasto energético total (ETE), MDA, 8-OHdG, TAC, NO e estresse oxidativo total (SG) foram avaliados pelo teste de pontuação Wisc-IQ pré-validado, teste BOT-2, questionário de PA e técnicas de imunoensaio, respectivamente. RESULTADOS Os escores do conjunto Wisc-IQ e BOT-2 do desempenho das habilidades intelectuais e motoras mostraram uma correlação significativa com o status da atividade física e a regulação dos radicais livres do estresse oxidativo em escolares com DI leve e moderada após 12 semanas de exercício moderado. O desempenho das habilidades intelectuais e motoras dos participantes correlacionou-se positivamente com o aumento dos escores de atividade TAC e aptidão física e negativamente com MDA, 8-OHdG, NO e Total-OS, respectivamente. Houve uma melhora significativa nas habilidades motoras, como áreas específicas de precisão motora fina, velocidade de corrida, agilidade, coordenação de membros superiores, força, coordenação bilateral e equilíbrio entre crianças em idade escolar após o programa de exercícios. A análise de regressão múltipla passo a passo dos parâmetros demográficos, do estado físico e do estresse oxidativo explicou em torno de 78,0 a 93,4% da variação da incapacidade intelectual entre os escolares. CONCLUSÃO O treinamento aeróbico moderado por 12 semanas tem um impacto positivo na melhoria da capacidade intelectual de escolares com DI por meio da modulação do status redox, melhora da aptidão física e proficiência em habilidades motoras.
Descritores: Aptidão Física
Deficiência Intelectual
-Oxirredução
Biomarcadores/metabolismo
Exercício Físico
Estresse Oxidativo/fisiologia
Limites: Humanos
Criança
Adolescente
Responsável: BR1.1 - BIREME


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Id: biblio-950886
Autor: Vorphal, María Alejandra; Bruna, Carola; Wandersleben, Traudy; Dagnino-Leone, Jorge; Lobos-González, Francisco; Uribe, Elena; Martínez-Oyanedel, José; Bunster, Marta.
Título: Molecular and functional characterization of ferredoxin NADP(H) oxidoreductase from Gracilaria chilensis and its complex with ferredoxin
Fonte: Biol. Res;50:39, 2017. tab, graf.
Idioma: en.
Projeto: FONDECYT.
Resumo: BACKGROUND: Ferredoxin NADP(H) oxidoreductases (EC 1.18.1.2) (FNR) are flavoenzymes present in photosynthetic organisms; they are relevant for the production of reduced donors to redox reactions, i.e. in photosynthesis, the reduction of NADP+ to NADPH using the electrons provided by Ferredoxin (Fd), a small FeS soluble protein acceptor of electrons from PSI in chloroplasts. In rhodophyta no information about this system has been reported, this work is a contribution to the molecular and functional characterization of FNR from Gracilaria chilensis, also providing a structural analysis of the complex FNR/Fd. METHODS: The biochemical and kinetic characterization of FNR was performed from the enzyme purified from phycobilisomes enriched fractions. The sequence of the gene that codifies for the enzyme, was obtained using primers designed by comparison with sequences of Synechocystis and EST from Gracilaria. 5'RACE was used to confirm the absence of a CpcD domain in FNRPBS of Gracilaria chilensis. A three dimensional model for FNR and Fd, was built by comparative modeling and a model for the complex FNR: Fd by docking. RESULTS: The kinetic analysis shows KMNADPH of 12.5 M and a kcat of 86 s-1, data consistent with the parameters determined for the enzyme purified from a soluble extract. The sequence for FNR was obtained and translated to a protein of 33646 Da. A FAD and a NADP+ binding domain were clearly identified by sequence analysis as well as a chloroplast signal sequence. Phycobilisome binding domain, present in some cyanobacteria was absent. Transcriptome analysis of Gch revealed the presence of two Fd; FdL and FdS, sharing the motif CX5CX2CX29X. The analysis indicated that the most probable partner for FNR is FdS. CONCLUSION: The interaction model produced, was consistent with functional properties reported for FNR in plants leaves, and opens the possibilities for research in other rhodophyta of commercial interest.
Descritores: Gracilaria/enzimologia
Ferredoxina-NADP Redutase/química
Ferredoxinas/metabolismo
-Oxirredução
Fotossíntese/fisiologia
Sequência de Aminoácidos
Gracilaria/química
Eletroforese em Gel de Poliacrilamida
Ferredoxina-NADP Redutase/genética
Ferredoxina-NADP Redutase/farmacocinética
Responsável: CL1.1 - Biblioteca Central



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