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Id: lil-703649
Autor: Pereira, Roberta Verciano; Vieira, Helaine Graziele Santos; Oliveira, Victor Fernandes de; Gomes, Matheus de Souza; Passos, Liana Konovaloff Jannotti; Borges, William de Castro; Guerra-Sa, Renata.
Título: Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni
Fonte: Mem. Inst. Oswaldo Cruz;109(1):1-8, 02/2014. tab, graf.
Idioma: en.
Projeto: FAPEMIG.
Resumo: Several genes related to the ubiquitin (Ub)-proteasome pathway, including those coding for proteasome subunits and conjugation enzymes, are differentially expressed during the Schistosoma mansoni life cycle. Although deubiquitinating enzymes have been reported to be negative regulators of protein ubiquitination and shown to play an important role in Ub-dependent processes, little is known about their role in S. mansoni . In this study, we analysed the Ub carboxyl-terminal hydrolase (UCHs) proteins found in the database of the parasite’s genome. An in silico ana- lysis (GeneDB and MEROPS) identified three different UCH family members in the genome, Sm UCH-L3, Sm UCH-L5 and Sm BAP-1 and a phylogenetic analysis confirmed the evolutionary conservation of the proteins. We performed quantitative reverse transcription-polymerase chain reaction and observed a differential expression profile for all of the investigated transcripts between the cercariae and adult worm stages. These results were corroborated by low rates of Z-Arg-Leu-Arg-Gly-Gly-AMC hydrolysis in a crude extract obtained from cercariae in parallel with high Ub conjugate levels in the same extracts. We suggest that the accumulation of ubiquitinated proteins in the cercaria and early schistosomulum stages is related to a decrease in 26S proteasome activity. Taken together, our data suggest that UCH family members contribute to regulating the activity of the Ub-proteasome system during the life cycle of this parasite.
Descritores: Endopeptidases/genética
Schistosoma mansoni/enzimologia
Ubiquitina Tiolesterase/genética
-Cercárias/enzimologia
Cercárias/genética
Sequência Conservada/genética
Evolução Molecular
Expressão Gênica
Genoma Helmíntico/genética
Genoma/genética
Estágios do Ciclo de Vida/genética
Camundongos Endogâmicos BALB C
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Alinhamento de Sequência
Schistosoma mansoni/genética
Schistosoma mansoni/crescimento & desenvolvimento
Transcriptoma/fisiologia
Transcitose/fisiologia
Ubiquitina Tiolesterase/classificação
Proteases Específicas de Ubiquitina/genética
Ubiquitinação/fisiologia
Limites: Animais
Responsável: BR1.1 - BIREME



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