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Pesquisa : G03.295.770.500 [Categoria DeCS]
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Id: biblio-889447
Autor: Spindola, Daniel Gonsales; Hinsberger, Andre; Antunes, Valéria Maria de Souza; Michelin, Luis Felipe Gomes; Bincoletto, Claudia; Oliveira, Carlos Rocha.
Título: In vitro cytotoxicity of chemical preservatives on human fibroblast cells
Fonte: Braz. J. Pharm. Sci. (Online);54(1):e00031, 2018. graf.
Idioma: en.
Resumo: Preservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MTT assay and propidium iodide staining (IMU>methylparaben>sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in the treated cells. Methylparaben and sodium benzoate were shown to have a very low cytotoxic activity. Taken together, our results suggest that IMU induces programed cell death in human fibroblasts by a canonical intrinsic pathway via mitochondrial perturbation and subsequent release of proapoptotic factors
Descritores: Conservantes Farmacêuticos/análise
Aditivos em Cosméticos
-Ciclo Celular
Citotoxicidade Imunológica
Potencial da Membrana Mitocondrial
Fibroblastos
Citometria de Fluxo/métodos
Tipo de Publ: Técnicas In Vitro
Estudo Comparativo
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: lil-714336
Autor: Cabrera, Paulina; Sánchez, Raúl; Risopatrón, Jennie.
Título: Selección espermática en semen congelado/descongelado de equino: evaluación de las membranas plasmática, acrosomal y potencial de membrana mitocondrial / Sperm selection in frozen/thawed semen of equine: evaluation of plasma, acrosome membranes and mitochondrial membrane potential
Fonte: Int. j. morphol;32(2):725-731, jun. 2014. ilus.
Idioma: es.
Resumo: Los procedimientos de criopreservación inducen cambios morfofuncionales en los espermatozoides. Es importante post descongelación espermática utilizar procedimientos de selección que permitan recuperar espermatozoides altamente funcionales. El objetivo del presente estudio fue comparar la eficiencia del Swim-up y Equipure® en la selección de espermatozoides funcionales en semen descongelado de equino. Semen de 4 potros reproductores Criollos Chilenos (A, B, C y D), fueron descongelados separadamente y procesados (n=15) por: I.- Swim-up (SU) y II.- Equipure® (EQ). Post descongelación se determinó por citometría de flujo la viabilidad e integridad de membrana plasmática (SYBR-14/PI), potencial de membrana mitocondrial (YDm; JC-1), integridad de la membrana acrosomal (FITC-PSA/PI). La motilidad progresiva (%) en dos animales fue más alta (P<0,05) por SU comparado con EQ: A (55,7±5,8% v/s 38,17±3,7%) y C (37,5±7% vs. 32±2,1%, respectivamente). La integridad de la membrana plasmática (%), tres animales presentaron diferencias (P<0,05), siendo más alta por SU en dos animales comparado con EQ (A: 54,3±1,7 vs. 36,7±1,9, C: 36,1±5,7 vs. 29,4±4,8 y D: 34,4±9,4 vs. 52,7±5,2; respectivamente), solamente un animal fue superior EQ. En el YDm (%), diferencias significativas (P<0,05) fueron detectadas en los cuatro animales, siendo más altos en SU comparado con EQ (A: 69,1±8,6% vs. 47,4±3,3%, B: 59,34±12,3% vs. 24,8±1,5%, C: 54,9±12,3% vs. 43,2±3,1% y D: 53,1±17,6% vs. 37,5±5,7%; respectivamente). Los resultados obtenidos en el presente estudio demostraron que los métodos de selección espermática Swim-up y Equipure® permiten recuperar espermatozoides de diferente calidad funcional en semen congelado-descongelado de equino, presentándose diferencias individuales entre los animales con respecto a los métodos. Se observó una tendencia del método Swim-up en seleccionar espermatozoides de equino descongelados con mayor calidad funcional comparado con Equipure®.

Freeze-thaw procedures induce structural and functional changes in sperm. It is important to use post thaw sperm selection procedures that can retrieve highly functional sperm. The aim of this study was to compare the efficiency of the Swim-up and Equipure® in the selection of functional sperm of thawed equine semen. Semen of four Chilean Criollo reproductive stallions (A, B , C and D) were frozen and thawed using a standard protocol and processed separately (n = 15) : I. Swim-up (SU) and II. Equipure® (EQ). Post sperm selection,was determined by flow cytometry. Viability and plasma membrane integrity (SYRB-14/PI), mitochondrial membrane potential (YDm, JC -1), acrosome membrane integrity (FITC-PSA/PI). Progressive motility (%) was higher (P<0.05) in two animals per SU compared with EQ, A (55.7±5.8% vs. 38.17±3.7%) and C (37.5±7.0% vs. 32±2.1%, respectively). The viability and integrity of the plasma membrane (%), three animals showed differences (P<0.05), being higher for SU in two animals compared with EQ (A: 54.3±1.7 vs. 36.7±1.9, C: 36.1±5.7 vs. 29.4±4.8 and D: 34.4±9.4 vs. 52.7±5.2, respectively), only one animal was higher EQ. In YDm (%), significant differences were detected (P<0.05) in all four animals, being higher in SU compared with EQ (A: 69.1±8.6% vs. 47.4±3.3% B: 59.34±12.3% vs. 24.8±1.5%, C: 54.9±12.3% vs. 43.2±3.1% and D: 53.1±17.6% vs. 37.5±5.7%, respectively). The results obtained in this study showed that sperm selection methods Swim-up and Equipure® can retrieve different functional sperm quality in frozen-thawed equine semen, and that individual differences were registered among animals with respect to methods. In the Swim-up method a tendency for selecting higher functional quality in thawed equine sperm was observed when compared to Equipure®.
Descritores: Espermatozoides/fisiologia
Criopreservação/veterinária
Cavalos
-Preservação do Sêmen
Motilidade Espermática/fisiologia
Acrossomo/fisiologia
Membrana Celular/fisiologia
Potencial da Membrana Mitocondrial/fisiologia
Análise do Sêmen
Citometria de Fluxo
Limites: Animais
Masculino
Responsável: CL1.1 - Biblioteca Central


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Id: lil-694715
Autor: Pronsato, Lucia; La Colla, Anabela; Ronda, Ana Carolina; Milanesi, Lorena; Boland, Ricardo; Vasconsuelo, Andrea.
Título: High passage numbers induce resistance to apoptosis in C2C12 muscle cells
Fonte: Biocell;37(1):1-9, Apr. 2013. ilus, graf.
Idioma: en.
Resumo: Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.
Descritores: Apoptose/efeitos dos fármacos
Peróxido de Hidrogênio/farmacologia
Mitocôndrias/patologia
Mioblastos Esqueléticos/patologia
Oxidantes/farmacologia
-Western Blotting
Técnicas de Cultura de Células
Células Cultivadas
/metabolismo
CASPASE ABATTOIRS/metabolismo
Divisão Celular/efeitos dos fármacos
Imunoprecipitação
Microscopia de Fluorescência
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Mioblastos Esqueléticos/efeitos dos fármacos
Mioblastos Esqueléticos/metabolismo
/metabolismo
BCL-TEMEFOS-ASSOCIATED X PROTEIN/metabolismo
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-650570
Autor: Li, Yiwen; Xu, Yuqing; Lei, Bo; Wang, Wenxiu; Ge, Xin; Li, Jingrui.
Título: Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;45(11):1052-1059, Nov. 2012. ilus.
Idioma: en.
Resumo: Rhein is a primary anthraquinone found in the roots of a traditional Chinese herb, rhubarb, and has been shown to have some anticancer effects. The aim of the present study was to investigate the effect of rhein on the apoptosis of the human gastric cancer line SGC-7901 and to identify the mechanism involved. SGC-7901 cells were cultured and treated with rhein (0, 50, 100, 150, and 200 µM) for 24, 48, or 72 h. Relative cell viability assessed by the MTT assay after treatment was 100, 99, 85, 79, 63% for 24 h; 100, 98, 80, 51, 37% for 48 h, and 100, 97, 60, 36, 15% for 72 h, respectively. Cell apoptosis was detected with TUNEL staining and quantified with flow cytometry using annexin FITC-PI staining at 48 h after 100, 200 and 300 µm rhein. The percentage of apoptotic cells was 7.3, 21.9, 43.5%, respectively. We also measured the mRNA levels of caspase-3 and -9 using real-time PCR. Treatment with 100 µM rhein for 48 h significantly increased mRNA expression of caspase-3 and -9. The levels of apoptosis-related proteins including Bcl-2, Bax, Bcl-xL, and pro-caspase-3 were evaluated in rhein-treated cells. Rhein increased the Bax:Bcl-2 ratio but decreased the protein levels of Bcl-xL and pro-caspase-3. Moreover, rhein significantly increased the expression of cytochrome c and apoptotic protease activating factor 1, two critical components involved in mitochondrial pathway-mediated apoptosis. We conclude that rhein inhibits SGC-7901 proliferation by inducing apoptosis and this antitumor effect of rhein is mediated in part by an intrinsic mitochondrial pathway.
Descritores: Antraquinonas/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Neoplasias Gástricas/patologia
-Linhagem Celular Tumoral
Caspase 9/metabolismo
/metabolismo
CASPASE ABATTOIRS/metabolismo
Citometria de Fluxo
Marcação In Situ das Extremidades Cortadas
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Reação em Cadeia da Polimerase em Tempo Real
Neoplasias Gástricas/metabolismo
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-625263
Autor: Bruges, Gustavo; Betancourt, Meyerling; March, Mariana; Sanchez, Evangelina; Mijares, Alfredo.
Título: Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi / Actividad proapoptótica de la estaurosporina en cultivos axénicos de Trypanosoma evansi
Fonte: Rev. Inst. Med. Trop. Säo Paulo;54(2):103-108, Mar.-Apr. 2012. ilus.
Idioma: en.
Resumo: Trypanosoma evansi is a blood protozoan parasite of the genus Trypanosoma which is responsible for surra (Trypanosomosis) in domestic and wild animals. This study addressed apoptotic-like features in Trypanosoma evansi in vitro. The mechanism of parasite death was investigated using staurosporine as an inducing agent. We evaluated its effects through several cytoplasmic features of apoptosis, including cell shrinkage, phosphatidylserine exposure, maintenance of plasma membrane integrity, and mitochondrial trans-membrane potential. For access to these features we have used the flow cytometry and fluorescence microscopy with cultures in the stationary phase and adjusted to a density of 10(6) cells/mL. The apoptotic effect of staurosporine in T. evansi was evaluated at 20 nM final concentration. There was an increase of phosphatidylserine exposure, whereas mitochondrial potential was decreased. Moreover, no evidence of cell permeability increasing with staurosporine was observed in this study, suggesting the absence of a necrotic process. Additional studies are needed to elucidate the possible pathways associated with this form of cell death in this hemoparasite.

Trypanosoma evansi es un hemoparásito, el cual es el agente causal de la surra (tripanosomiasis) en mamíferos, perteneciente al orden Kinetoplastidae. Este estudio se oriento a caracterizar la muerte celular similar a apoptosis en cultivos in vitro de Trypanosoma evansi a través del uso del inductor esturosporina. Este efecto se evaluó a través de diversos aspectos fenotípicos de la apoptosis: el encogimiento celular, la exposición de fosfatidilserina, el mantenimiento de la integridad de la membrana plasmática y el potencial de membrana mitocondrial. Para evaluar estas características se utilizaron técnicas de citometría de flujo y microscopía de fluorescencia con cultivos en fase estacionaria ajustados a una densidad de 10(6) células/mL. El efecto apoptótico de la estaurosporina en Trypanosoma evansi fue evaluado a una concentración de 20 nM. Se evidenció un aumento de la exposición a fosfatidilserina, mientras que el potencial mitocondrial disminuyó. Por otra parte, no hay evidencias de aumento de la permeabilidad celular con estaurosporina, sugiriendo la ausencia de un proceso necrótico. Estudios adicionales son necesarios para dilucidar las posibles vías asociadas con esta forma de muerte celular en este hemoparásito.
Descritores: Apoptose
Inibidores Enzimáticos/farmacologia
Estaurosporina/farmacologia
Trypanosoma/efeitos dos fármacos
-Cultura Axênica
Citometria de Fluxo
Microscopia de Fluorescência
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Trypanosoma/enzimologia
Responsável: BR1.1 - BIREME


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Id: lil-604283
Autor: Meinerz, D. F; de Paula, M. T; Comparsi, B; Silva, M. U; Schmitz, A. E; Braga, H. C; Taube, P. S; Braga, A. L; Rocha, J. B. T; Dafre, A. L; Farina, M; Franco, J. L; Posser, T.
Título: Protective effects of organoselenium compounds against methylmercury-induced oxidative stress in mouse brain mitochondrial-enriched fractions
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;44(11):1156-1163, Nov. 2011. ilus.
Idioma: en.
Resumo: We evaluated the potential neuroprotective effect of 1-100 µM of four organoselenium compounds: diphenyl diselenide, 3’3-ditri-fluoromethyldiphenyl diselenide, p-methoxy-diphenyl diselenide, and p-chloro-diphenyl diselenide, against methylmercury-induced mitochondrial dysfunction and oxidative stress in mitochondrial-enriched fractions from adult Swiss mouse brain. Methylmercury (10-100 µM) significantly decreased mitochondrial activity, assessed by MTT reduction assay, in a dose-dependent manner, which occurred in parallel with increased glutathione oxidation, hydroperoxide formation (xylenol orange assay) and lipid peroxidation end-products (thiobarbituric acid reactive substances, TBARS). The co-incubation with diphenyl diselenide (100 µM) completely prevented the disruption of mitochondrial activity as well as the increase in TBARS levels caused by methylmercury. The compound 3’3-ditrifluoromethyldiphenyl diselenide provided a partial but significant protection against methylmercury-induced mitochondrial dysfunction (45.4 ± 5.8 percent inhibition of the methylmercury effect). Diphenyl diselenide showed a higher thiol peroxidase activity compared to the other three compounds. Catalase blocked methylmercury-induced TBARS, pointing to hydrogen peroxide as a vector during methylmercury toxicity in this model. This result also suggests that thiol peroxidase activity of organoselenium compounds accounts for their protective actions against methylmercury-induced oxidative stress. Our results show that diphenyl diselenide and potentially other organoselenium compounds may represent important molecules in the search for an improved therapy against the deleterious effects of methylmercury as well as other mercury compounds.
Descritores: Encéfalo/efeitos dos fármacos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Intoxicação do Sistema Nervoso por Mercúrio/prevenção & controle
Compostos de Metilmercúrio/toxicidade
Mitocôndrias/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Compostos Organosselênicos/farmacologia
Estresse Oxidativo/efeitos dos fármacos
-Análise de Variância
Derivados de Benzeno/farmacologia
Fracionamento Celular
Modelos Animais
Fármacos Neuroprotetores/classificação
Compostos Organosselênicos/química
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-604196
Autor: Barbosa, Ailton Jose; Santana, Ana Carolina Delazia Albuquerque; Castro e Silva, Tiago; Kurachi, Cristina; Inada, Natalia; Bagnato, Vanderlei Salvador; Silva Júnior, Orlando de Castro e.
Título: Effect of laser on the remnant liver after the first 24 hours following 70 percent hepatectomy in rats / Efeito do laser no fígado remanescente nas primeiras 24 horas após hepatectomia a 70 por cento em ratos
Fonte: Acta cir. bras;26(6):470-474, Nov.-Dec. 2011. ilus.
Idioma: en.
Resumo: PURPOSE: To evaluate the mitochondrial function of the remnant liver (RL) in the early phase of liver regeneration in rats after 70 percent partial hepatectomy (PH). METHODS: Sixty male Wistar rats (200-250g) submitted to 70 percent PH were divided into five groups according to the time of euthanasia and application or not of laser light: C = Control, time zero; 2 minutes, 4, 6 and 24 hours after PH. The dose of laser radiation was 22.5 J/cm², wavelength of 660 nm (visible/red), in the remnant liver. We studied the respiration activated by ADP (state 3), basal mitochondrial respiration (state 4), respiratory control ratio (RCR) and mitochondrial membrane potential (MMP). RESULTS: The mitochondrial function of RL changed at 4 and 6 hours after PH, with a significant increase in state 3 and a concomitant increase in state 4 and with maintenance of RCR. MMP differed significantly between the groups biostimulated with laser radiation and the control group 4 hours after HP, with a substantial reduction in the non-laser groups. CONCLUSION: The laser light at the dose used in this study did not induce additional damage to the RL and seems to have delayed the hepatocellular metabolic overload of the remnant liver.

OBJETIVO: Avaliar a função mitocondrial do fígado remanescente (FR) na fase precoce da regeneração hepática em ratos após hepatectomia parcial (HP) a 70 por cento. MÉTODOS: Sessenta ratos machos Wistar (200 - 250g) submetidos à HP a 70 por cento, foram distribuídos em cinco grupos de acordo com o tempo de eutanásia e com aplicação ou não de luz laser: C= Controle,tempo zero; 2 minutos, 4, 6 e 24 horas após HP. O laser foi utilizado na dose 22.5 J/cm², 660 nm, no FR.Estudou-se o estado 3 (respiração ativada por ADP), estado 4 (respiração mitocondrial basal), razão de controle respiratório,estado 3/estado 4 (RCR) e o potencial de membrana mitocondrial(PMM). RESULTADOS: A função mitocondrial do FR alterou-se no período de 4 e 6 horas após a HP com aumento significativo do estado 3 e aumento concomitante do estado 4, com manutenção da RCR. O PMM apresentou diferença significativa entre os grupos bioestimulados com laser e o controle a partir de 4 horas pós HP, com queda importante do grupo sem laser e tendência a equiparação dos valores após 24 horas. CONCLUSÃO: A luz laser, na dose utilizada no presente estudo, não induziu lesão adicional ao FR e parece ter retardado a sobrecarga hepatocelular do fígado remanescente.
Descritores: Hepatectomia/métodos
Terapia a Laser/métodos
Regeneração Hepática/efeitos da radiação
Potencial da Membrana Mitocondrial/efeitos da radiação
Mitocôndrias Hepáticas/efeitos da radiação
-Fígado/metabolismo
Fígado/efeitos da radiação
Consumo de Oxigênio/efeitos da radiação
Ratos Wistar
Fatores de Tempo
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-586506
Autor: Song, Rui; Bian, Hui-Ning; Lai, Wen; Chen, Hua-De; Zhao, Ke-Seng.
Título: Normal skin and hypertrophic scar fibroblasts differentially regulate collagen and fibronectin expression as well as mitochondrial membrane potential in response to basic fibroblast growth factor
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;44(5):402-410, May 2011. ilus.
Idioma: en.
Resumo: Basic fibroblast growth factor (bFGF) regulates skin wound healing; however, the underlying mechanism remains to be defined. In the present study, we determined the effects of bFGF on the regulation of cell growth as well as collagen and fibronectin expression in fibroblasts from normal human skin and from hypertrophic scars. We then explored the involvement of mitochondria in mediating bFGF-inducedeffects on the fibroblasts. We isolated and cultivated normal and hypertrophic scar fibroblasts from tissue biopsies of patients who underwent plastic surgery for repairing hypertrophic scars. The fibroblasts were then treated with different concentrations of bFGF (ranging from 0.1 to 1000 ng/mL). The growth of hypertrophic scar fibroblasts became slower with selective inhibition of type I collagen production after exposure to bFGF. However, type III collagen expression was affected in both normal and hypertrophic scar fibroblasts. Moreover, fibronectin expression in the normal fibroblasts was up-regulated after bFGF treatment. bFGF (1000 ng/mL) also induced mitochondrial depolarization in hypertrophic scar fibroblasts (P < 0.01). The cellular ATP level decreased in hypertrophic scar fibroblasts (P < 0.05), while it increased in the normal fibroblasts following treatment with bFGF (P < 0.01). These data suggest that bFGF has differential effects and mechanisms on fibroblasts of the normal skin and hypertrophic scars, indicating that bFGF may play a role in the early phase of skin wound healing and post-burn scar formation.
Descritores: Cicatriz Hipertrófica/patologia
Colágeno Tipo I/metabolismo
Colágeno Tipo III/metabolismo
/farmacologia
FIBROBLAST GROWTH FACTOR TEMEFOS/farmacologia
Fibroblastos/efeitos dos fármacos
Fibronectinas/metabolismo
Pele/citologia
-Células Cultivadas
Cicatriz Hipertrófica/metabolismo
Colágeno Tipo I/ultraestrutura
Colágeno Tipo III/ultraestrutura
Fibroblastos/metabolismo
Fibroblastos/ultraestrutura
Fibronectinas/ultraestrutura
Microscopia Eletrônica de Transmissão
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Potencial da Membrana Mitocondrial/fisiologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Pele/metabolismo
Regulação para Cima
Cicatrização
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-438800
Autor: Pacheco, Eduardo Garcia; Gomes, Maria Cecília Jordani; Rodrigues, Gustavo Ribeiro; Campos, Walter; Kemp, Rafael; Castro e Silva, Orlando de.
Título: Effect of liver ischemic preconditioning in cirrhotic rats submitted to hepatic ischemia/reperfusion injury / Efeito do pré-condicionamento isquêmico hepático submetidos a lesão de isquemia/reperfusão do fígado
Fonte: Acta cir. bras;21(supl.1):24-28, 2006. graf.
Idioma: en; pt.
Projeto: CNPq; . FAPESP.
Resumo: PURPOSE: The main aim of this study was to determine the influence of ischemic preconditioning (IPC) on rat liver cirrhosis. METHODS: Cirrhosis was induced in Wistar rats by occlusion of the hepatic duct. The animals were divided into four groups of six animals each: non-cirrhotic group (simulated operation only), cirrhotic control group (simulated operation in cirrhotic rats), I/R group (40-minute ischemia without IPC), and IPC group (cirrhotic rats with ischemia, previously submitted to IPC). The IPC procedure consisted of partial hepatic ischemia for five minutes, followed by 10 minutes of reperfusion. In the case of the IPC group, the animals were submitted to liver ischemia for 40 minutes after the preconditioning procedure, followed by 2 hours of reperfusion. Blood samples were collected for measurement of serum aminotransferases (ALT and AST). The respiratory control ratio (RCR), the mitochondrial membrane potential (MMP), and malondialdehyde (MDA) values in the hepatic tissue were analyzed. Nonparametric statistical analysis was used and a value of p<0.05 was considered statistically significant. RESULTS: Ischemia did not induce significant increase in ALT and AST levels. MDA values were significantly higher in cirrhotic animals. MMP did not significantly change in cirrhosis and liver ischemia. Mitochondrial RCR decreased in liver cirrhosis, accentuated upon liver ischemia, and did not significantly change with IPC. CONCLUSION: Ischemic preconditioning does not protect the liver from hepatic injury induced by the ischemia/ reperfusion process.

OBJETIVO: O objetivo deste estudo foi determinar a influência do pré-condicionamento isquêmico (IPC) em fígados de ratos cirróticos. MÉTODOS: A cirrose hepática foi induzida em ratos Wistar machos (250 a 300g) por oclusão, durante 30 dias, do ducto hepático comum.A seguir, os animais cirróticos foram divididos em três grupos de seis; Grupo controle cirrótico (operação simulada para isquemia/reperfusão/pré-condicionamento), Grupo I/R, isquemia de 40 minutos sem pré-condicionamento (IPC) e grupo IPC com isquemia precedida por IPC. O IPC consistiu de uma isquemia parcial por cinco minutos, seguida por 10 minutos de reperfusão. No grupo IPC, após o pré-condicionamento, os animais foram submetidos à isquemia hepática de 40 minutos seguida de 2 horas de reperfusão. Foram colhidas amostras de sangue para dosagem sérica de aminotransferases (ALT e AST). Razão de controle respiratório (RCR), potencial de membrana das mitocôndrias (PMM) e dosagem de malondialdeído (MDA) foram analisadas no tecido hepático. Analise estatística não paramétrica foi utilizada com nível de significância de 5 por cento para as comparações entre grupos. RESULTADOS: A isquemia não induziu aumento significativo das aminotransferases. Houve aumento significativo de MDA nos animais cirróticos.O PMM não se alterou significativamente na cirrose e na isquemia hepática. A RCR mitocondrial diminuiu na cirrose hepática e acentuou-se com a isquemia do fígado e não se alterou significativamente com o IPC. CONCLUSÃO: O pré-condicionamento isquêmico não protegeu o fígado das lesões hepáticas induzidas pelo processo de isquemia/reperfusão.
Descritores: Precondicionamento Isquêmico
Cirrose Hepática Experimental/metabolismo
Fígado/irrigação sanguínea
Traumatismo por Reperfusão/metabolismo
-Modelos Animais de Doenças
Testes de Função Hepática
Fígado/química
Malondialdeído/análise
Potencial da Membrana Mitocondrial/fisiologia
Mitocôndrias Hepáticas/química
Ratos Wistar
Estatísticas não Paramétricas
Transaminases/sangue
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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