Base de dados : LILACS
Pesquisa : G04.022 [Categoria DeCS]
Referências encontradas : 206 [refinar]
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Id: lil-794669
Autor: Zhu, Yong-tong; Pang, Shi-yu; Luo, Yang; Chen, Wei; Bao, Ji-ming; Tan, Wan-long.
Título: A modified method by differential adhesion for enrichment of bladder cancer stem cells
Fonte: Int. braz. j. urol;42(4):817-824, July-Aug. 2016. tab, graf.
Idioma: en.
Projeto: Education Department in Guangdong Province; . Southern Medical University.
Resumo: ABSTRACT Purpose: In a previous study the vaccine was effective against bladder cancer in a mouse model. However, a small portion of tumors regrew because the vaccine could not eliminate bladder cancer stem cells (CSCs). In this study, we showed a modified method for the isolation of bladder CSCs using a combination of differential adhesion method and serum-free culture medium (SFM) method. Materials and Methods: Trypsin-resistant cells and trypsin-sensitive cells were isolated from MB49, EJ and 5637 cells by a combination of differential adhesion method and SFM method. The CSCs characterizations of trypsin-resistant cells were verified by the flow cytometry, the western blotting, the quantitative polymerase chain reaction, the resistance to chemotherapy assay, the transwell assay, and the tumor xenograft formation assay. Results: Trypsin-resistant cells were isolated and identified in CSCs characters, with high expression of CSCs markers, higher resistance to chemotherapy, greater migration in vitro, and stronger tumorigenicity in vivo. Conclusion: Trypsin-resistant cells displayed specific CSCs properties. Our study showed trypsin-resistant cells were isolated successfully with a modified method using a combination of differential adhesion method and SFM method.
Descritores: Células-Tronco Neoplásicas/citologia
Neoplasias da Bexiga Urinária/patologia
Tripsina/farmacologia
Adesão Celular/efeitos dos fármacos
Separação Celular/métodos
Técnicas de Cultura de Células/métodos
-Células-Tronco Neoplásicas/química
Biomarcadores Tumorais
Diferenciação Celular
Meios de Cultura Livres de Soro
Vacinas Anticâncer/imunologia
Linhagem Celular Tumoral
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Camundongos Nus
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


  2 / 206 LILACS  
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Id: biblio-989635
Autor: Ghani, Siti Madiani Abdul; Goon, Jo Aan; Azman, Nor Helwa Ezzah Nor; Zakaria, Siti Nor Asyikin; Hamid, Zalina; Ngah, Wan Zurinah Wan.
Título: Comparing the effects of vitamin E tocotrienol-rich fraction supplementation and α-tocopherol supplementation on gene expression in healthy older adults
Fonte: Clinics;74:e688, 2019. tab, graf.
Idioma: en.
Projeto: UKM Economic Transformation Programme.
Resumo: OBJECTIVES This study aims to compare the differential gene expression resulting from tocotrienol-rich fraction and α-tocopherol supplementation in healthy older adults. METHODS A total of 71 eligible subjects aged 50 to 55 years from Gombak and Kuala Lumpur, Malaysia, were divided into three groups and supplemented with placebo (n=23), α-tocopherol (n=24) or tocotrienol-rich fraction (n=24). Blood samples were collected at baseline and at 3 and 6 months of supplementation for microarray analysis. RESULTS The number of genes altered by α-tocopherol was higher after 6 months (1,410) than after 3 months (273) of supplementation. α-Tocopherol altered the expression of more genes in males (952) than in females (731). Similarly, tocotrienol-rich fraction modulated the expression of more genes after 6 months (1,084) than after 3 months (596) and affected more genes in males (899) than in females (781). α-Tocopherol supplementation modulated pathways involving the response to stress and stimuli, the immune response, the response to hypoxia and bacteria, the metabolism of toxins and xenobiotics, mitosis, and synaptic transmission as well as activated the mitogen-activated protein kinase and complement pathways after 6 months. However, tocotrienol-rich fraction supplementation affected pathways such as the signal transduction, apoptosis, nuclear factor kappa B kinase, cascade extracellular signal-regulated kinase-1 and extracellular signal-regulated kinase-2, immune response, response to drug, cell adhesion, multicellular organismal development and G protein signaling pathways. CONCLUSION Supplementation with either α-tocopherol or tocotrienol-rich fraction affected the immune and drug response and the cell adhesion and signal transduction pathways but modulated other pathways differently after 6 months of supplementation, with sex-specific responses.
Descritores: Expressão Gênica/efeitos dos fármacos
Suplementos Nutricionais
alfa-Tocoferol/farmacologia
Tocotrienóis/farmacologia
Antioxidantes/farmacologia
-Proteínas Quinases/efeitos dos fármacos
Fatores de Tempo
Transdução de Sinais/efeitos dos fármacos
Adesão Celular/efeitos dos fármacos
Método Simples-Cego
Fatores Sexuais
Regulação da Expressão Gênica/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Sistema Imunitário/efeitos dos fármacos
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Tipo de Publ: Estudo Comparativo
Ensaio Clínico Controlado Aleatório
Responsável: BR1.1 - BIREME


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Id: lil-792796
Autor: Singh, Shweta; Fatima, Zeeshan; Hameed, Saif.
Título: Citronellal-induced disruption of membrane homeostasis in candida albicans and attenuation of its virulence attributes
Fonte: Rev. Soc. Bras. Med. Trop;49(4):465-472, July-Aug. 2016. graf.
Idioma: en.
Projeto: Science and Engineering Research Board.
Resumo: Abstract: INTRODUCTION There is an increasing burden of multidrug resistance. As a result, deciphering the mechanisms of action of natural compounds with antifungal activity has gained considerable prominence. We aimed to elucidate the probable mechanism of action of citronellal, a monoterpenoid found in the essential oil extracted from Cymbopogon plants, against Candida albicans. METHODS Drug susceptibility was measured by broth microdilution and spot assays. Ergosterol levels were estimated using the alcoholic potassium hydroxide method and H+ extrusion was assessed by monitoring the glucose-induced acidification of the external medium. Virulence traits were studied by hyphal morphogenesis and biofilm formation, along with fungal cell adherence to polystyrene surface and human oral epithelial cells. RESULTS Citronellal showed anticandidal activity against C. albicans and non-albicans species of Candida at a minimum inhibitory concentration of 1 mg/ml. Citronellal interfered with membrane homeostasis, which is the major target of known antifungal drugs, by increasing the hypersensitivity of the fungi to membrane-perturbing agents, reducing ergosterol levels, and diminishing glucose-induced H+ extrusion. In addition, oxidative and genotoxic stresses were induced via an increased production of reactive oxygen species. Furthermore, citronellal inhibited the virulent attributes of yeast-to-hypha transition and biofilm formation. It also reduced cell adherence to polystyrene surface and the human oral epithelial cells. CONCLUSIONS This is the first study to propose the cell membrane, morphogenetic switching, biofilm formation, and cell adherence of Candida albicans as potential targets for the anticandidal activity of citronellal. However, clinical investigations on the therapeutic applications of citronellal are required.
Descritores: Virulência/efeitos dos fármacos
Candida albicans/efeitos dos fármacos
Monoterpenos/farmacologia
Aldeídos/farmacologia
Homeostase/efeitos dos fármacos
Antifúngicos/farmacologia
-Candida albicans/patogenicidade
Testes de Sensibilidade Microbiana
Adesão Celular/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
Monoterpenos Acíclicos
Limites: Humanos
Responsável: BR1.1 - BIREME


  4 / 206 LILACS  
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Id: biblio-829669
Autor: Romero, Cintia Mariana; Vivacqua, Cristian Germán; Abdulhamid, María Belén; Baigori, Mario Domingo; Slanis, Alberto Carlos; Allori, María Cristina Gaudioso de; Tereschuk, María Laura.
Título: Biofilm inhibition activity of traditional medicinal plants from Northwestern Argentina against native pathogen and environmental microorganisms
Fonte: Rev. Soc. Bras. Med. Trop;49(6):703-712, Dec. 2016. tab, graf.
Idioma: en.
Projeto: Agencia de Promoción Científica y Tecnológica; . Programa de Investigación de la Universidad Nacional de Tucumán.
Resumo: Abstract: INTRODUCTION: Plants have been commonly used in popular medicine of most cultures for the treatment of disease. The in vitro antimicrobial activity of certain Argentine plants used in traditional medicine has been reported. The aim of this study was to investigate the antimicrobial, anti-biofilm, and anti-cell adherence activities of native plants (Larrea divaricata, Tagetes minuta, Tessaria absinthioides, Lycium chilense, and Schinus fasciculatus) collected in northwestern Argentina. METHODS: The activities of the five plant species were evaluated in Bacillus strains and clinical strains of coagulase-negative Staphylococcus isolated from northwestern Argentina and identified by 16S rDNA. RESULT: Lycium chilense and Schinus fasciculatus were the most effective antimicrobial plant extracts (15.62µg/ml and 62.50µg/ml for Staphylococcus sp. Mcr1 and Bacillus sp. Mcn4, respectively). The highest (66%) anti-biofilm activity against Bacillus sp. Mcn4 was observed with T. absinthioides and L. divaricate extracts. The highest (68%) anti-biofilm activity against Staphylococcus sp. Mcr1 was observed with L. chilense extract. T. minuta, T. absinthioides, and L. divaricata showed percentages of anti-biofilm activity of between 55% and 62%. The anti-adherence effects of T. minuta and L. chilense observed in Bacillus sp. Mcn4 reflected a difference of only 22% and 10%, respectively, between anti-adherence and biofilm inhibition. Thus, the inhibition of biofilm could be related to cell adherence. In Staphylococcus sp. Mcr1, all plant extracts produced low anti-adherence percentages. CONCLUSION: These five species may represent a source of alternative drugs derived from plant extracts, based on ethnobotanical knowledge from northwest Argentina.
Descritores: Plantas Medicinais/química
Staphylococcus/efeitos dos fármacos
Bacillus/efeitos dos fármacos
Extratos Vegetais/farmacologia
Biofilmes/efeitos dos fármacos
-Argentina
Plantas Medicinais/classificação
Testes de Sensibilidade Microbiana
Adesão Celular/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Anacardiaceae/química
Tagetes/química
Lycium/química
Larrea/química
Microbiologia Ambiental
Limites: Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: biblio-950798
Autor: Berrocal, Liliana; Fuentes, Juan A; Nicole Trombert, A; Jofré, Matías R; Villagra, Nicolás A; Valenzuela, Luis M; Mora, Guido C.
Título: stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
Fonte: Biol. Res;48:1-8, 2015. graf.
Idioma: en.
Projeto: Government of Chile. National Fund for Development of Science and Technology; . UNAB.
Resumo: BACKGROUND: Salmonella enterica serovar Typhi (S. Typhi) stg operon, encoding a chaperone/usher fimbria (CU), contributes to an increased adherence to human epithelial cells. However, one report suggests that the presence of the Stg fimbria impairs the monocyte-bacteria association, as deduced by the lower level of invasion to macrophage-like cells observed when the stg fimbrial cluster was overexpressed. Nevertheless, since other CU fimbrial structures increase the entry of S. Typhi into macrophages, and considering that transcriptomic analyses revealed that stg operon is indeed expressed in macrophages, we reassessed the role of the stg operon in the interaction between S. Typhi strain STH2370 and human cells, including macrophage-like cells and mononuclear cells directly taken from human peripheral blood. RESULTS: We compared S. Typhi STH2370 WT, a Chilean clinical strain, and the S. Typhi STH2370 Astg mutant with respect to association and invasion using epithelial and macrophage-like cells. We observed that deletion of stg operon reduced the association and invasion of S. Typhi, in both cellular types. The presence of the cloned stg operon restored the WT phenotype in all the cases. Moreover, we compared Salmonella enterica sv. Typhimurium 14028s (S. Typhimurium, a serovar lacking stg operon) and S. Typhimurium heterologously expressing S. Typhi stg. We found that the latter presents an increased cell disruption of polarized epithelial cells and an increased association in both epithelial and macrophage-like cells. CONCLUSIONS: S. Typhi stg operon encodes a functional adhesin that participates in the interaction bacteria-eukary-otic cells, including epithelial cells and macrophages-like cells. The phenotypes associated to stg operon include increased association and consequent invasion in bacteria-eukaryotic cells, and cell disruption.
Descritores: Óperon/fisiologia
Óperon/genética
Salmonella typhi/genética
Fímbrias Bacterianas/genética
Células Epiteliais/microbiologia
Macrófagos/microbiologia
-Salmonella typhi/fisiologia
Adesão Celular
Fímbrias Bacterianas/fisiologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1057282
Autor: Ataides, Fábio Silvestre; Costa, Carolina Rodrigues; Santos, Andressa Santana; Freitas, Vivianny Aparecida Queiroz; Silva, Thaisa Cristina; Zara, Ana Laura Sene Amâncio; Jesuino, Rosália Santos Amorim; Silva, Maria Rosário Rodrigues.
Título: In vitro characterization of virulence factors among species of the Candida parapsilosis complex
Fonte: Rev. Soc. Bras. Med. Trop;53:e20190336, 2020. tab, graf.
Idioma: en.
Projeto: National Counsel of Technological and Scientific Development.
Resumo: Abstract INTRODUCTION: Candida parapsilosis complex species differ from each other with regard to their prevalence and virulence. METHODS: The hydrolytic enzyme activity, biofilm production, and adhesion to epithelial cells were analyzed in 87 C. parapsilosis complex strains. RESULTS: Among the studied isolates, 97.7%, 63.2%, and 82.8% exhibited very strong proteinase, esterase, and hemolysin activity, respectively. All the C. parapsilosis complex isolates produced biofilms and presented an average adherence of 96.0 yeasts/100 epithelial cells. CONCLUSIONS: Our results show that Candida parapsilosis complex isolates showed different levels of enzyme activity, biofilm production, and adhesion to epithelial cells.
Descritores: Fatores de Virulência/análise
Candida parapsilosis/patogenicidade
-Adesão Celular
Técnicas de Tipagem Micológica
Biofilmes/crescimento & desenvolvimento
Candida parapsilosis/isolamento & purificação
Candida parapsilosis/classificação
Candida parapsilosis/enzimologia
Hidrolases/biossíntese
Limites: Humanos
Responsável: BR1.1 - BIREME


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Alves Júnior, Clodomiro
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Id: lil-744265
Autor: Coelho, Keylla Dayanne; Alves Junior, Clodomiro; Rocha, Hugo Alexandre de Oliveira; Queiroz Neto, Moacir Francisco de; Vitoriano, Jussier Oliveira; Silva, José Sandro Pereira da.
Título: Influência da composição dos gases durante a modificação da superfície por plasma na adesão de células primárias da polpa dental / Influence of the composition of gases during plasma surface modification on adhesion of primary cells from dental pulp
Fonte: Rev. bras. odontol;71(1):67-71, Jan.-Jun. 2014. ilus, tab.
Idioma: pt.
Resumo: Amostras de titânio foram nitretadas por plasma usando duas composições gasosas: 80%H2-20%N2 e 20%H2-80%N2 e as propriedades físicas químicas desse tratamento foram estudas, bem como sua resposta biológica quando submetidas à cultura de células primárias da polpa dentária. Análise por difração de raios X indicam a formação de TiN nas duas amostras nitretadas ao contrário da superfície não nitretada que continha apenas a fase Ti-α. Verificaram-se modificações na dureza, topografia e molhabilidade dessas amostras quando comparadas às amostras não nitretadas. A adesão celular nessas amostras indica que o tratamento aumenta seu valor sendo superior para amostras tratadas em maior concentração de hidrogênio.

Samples of titanium were nitrided using plasma and a composition of two gases: 80% H2-20% N2 and 20% H2-80% N2. The influence of these treatments on their physical chemical properties and biological response when subjected to the primary cell culture of dental pulp were studied. Analyses by X-ray diffraction indicate the formation of TiN in both nitrided samples in contrast to the non-nitrided surface samples that contained only the Ti-α phase. Changes in hardness, topography and wettability of these samples compared to non-nitrided samples were found as well. Finally, the analysis of cell's adhesion in these samples indicate that treatment increases its value being higher for samples treated at higher hydrogen concentration.
Descritores: Adesão Celular
Cultura Primária de Células
Gases
-Plasma
Titânio
Responsável: BR485.1 - Biblioteca Mário Badan


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Id: biblio-950863
Autor: Lins, Marvin Paulo; Vieira, Larissa Fernanda de Araújo; Rosa, Alfredo Aurélio Marinho; Smaniotto, Salete.
Título: Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
Fonte: Biol. Res;49:1-9, 2016. ilus, graf.
Idioma: en.
Resumo: BACKGROUND: Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocytes and thymic epithelial cells (TEC) in the presence of laminin. RESULTS: GH increased thymocyte adhesion on BSA-coated and further on laminin-coated surfaces. The number of migrating cells in laminin-coated membrane was higher in GH-treated thymocyte group. In both results, VLA-6 expression on thymocytes was constant. Also, treatment with GH enhanced laminin production by TEC after 24 h in culture. However, VLA-6 integrin expression on TEC remained unchanged. Finally, TEC/thymocyte co-culture model demonstrated that GH elevated absolute number of double-negative (CD4-CD8-) and single-positive CD4+ and CD8+ thymocytes. A decrease in cell number was noted in double-positive (CD4+CD8+) thymocytes. CONCLUSIONS: The results of this study demonstrate that GH is capable of enhancing the migratory capacity of human thymocytes in the presence of laminin and promotes modulation of thymocyte subsets after co-culture with TEC.
Descritores: Timo/citologia
Hormônio do Crescimento/farmacologia
Laminina/biossíntese
Células Epiteliais/efeitos dos fármacos
Timócitos/efeitos dos fármacos
-Valores de Referência
Timo/metabolismo
Fatores de Tempo
Imuno-Histoquímica
Linfócitos T CD4-Positivos
Adesão Celular/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Movimento Celular/efeitos dos fármacos
Células Cultivadas
Análise de Variância
Laminina/efeitos dos fármacos
Linfócitos T CD8-Positivos
Técnicas de Cocultura
Integrina alfa6beta1/análise
Integrina alfa6beta1/metabolismo
Citometria de Fluxo/métodos
Limites: Humanos
Recém-Nascido
Lactente
Pré-Escolar
Criança
Tipo de Publ: Estudo de Avaliação
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-870237
Autor: Andrade, Juliana Bartholo de.
Título: Co-expressão de CD44 e proteínas da família ERM (Ezrina/Moesina) no carcinoma de pênis / Co-expression of CD44 and ERM family proteins (Ezrin/Moesin) in penile carcinoma.
Fonte: São Paulo; s.n; 2015. 113 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: Na história natural do carcinoma de pênis, o envolvimento loco regional é considerado a complicação mais grave e principal causa de morte da doença. Compreender os processos moleculares que possam estar envolvidos nos mecanismos de invasão e disseminação metastática pode levar a um aprimoramento das ferramentas de prognóstico para uma melhor avaliação dos pacientes, evitando-se, assim, a morbidade de cirurgias desnecessárias. Alguns estudos têm enfatizado a influência da adesão celular no desenvolvimento tumoral e metastático, sendo as proteínas do complexo CD44/ERM (Ezrina, Radixina e Moesina) relatadas como envolvidas nestes processos. Objetivos: Analisar a co-expressão de CD44- Ezrina e de CD44-Moesina no carcinoma epidermóide de pênis, correlacionando esta co-expressão aos critérios clínico patológicos. Casuística, Materiais e Métodos: Foram estudados, retrospectivamente 76 pacientes submetidos à cirurgia para carcinoma epidermóide de pênis no AC Camargo Cancer Center. A avaliação das expressões isoladas de CD44, CD44V6, Ezrina e Moesina foi realizada por imunoistoquímica, no equipamento Ventana®, e a imunorreatividade de cada proteína foi categorizada em positiva e negativa. As co-expressões de Ezrina/CD44, Ezrina/CD44V6, Moesina/CD44 e Moesina/CD44V6 foram avaliadas...

The loco regional involvement is considered the most severe complication and main cause of death in the natural history of penile carcinoma. The understanding of molecular processes that may be involved in the invasion mechanism and metastatic dissemination would improve the prognostic tools to a better patient evaluation, avoiding the morbidity of unnecessary surgery. Some studies have emphasized the influence of cellular adhesion in the tumor and metastatic development, being the complex CD44/ERM proteins (Ezrin/Radixin/Moesin), reported as involved in these processes. Objectives: To analyze the co-expression of CD44-Ezrin and CD44-Moesin in penile squamous cell carcinoma, correlating this co-expression to pathological clinical criteria. Samples, Materials and Methods: We retrospectively studied 76 patients undergoing surgery for penile squam...
Descritores: Adesão Celular
ANTIGENOS CDABSENTEEISM
Carcinoma
Neoplasias Penianas
Limites: Humanos
Responsável: BR30.1 - Biblioteca
BR30.1


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Id: biblio-983943
Autor: Chae, Dong-Sik; Lee, Chang Youn; Lee, Jiyun; Seo, Hyang-Hee; Choi, Chong-Hyuk; Lee, Seahyoung; Hwang, Ki-Chul.
Título: Priming stem cells with protein kinase C activator enhances early stem cell-chondrocyte interaction by increasing adhesion molecules
Fonte: Biol. Res;51:41, 2018. graf.
Idioma: en.
Projeto: Korea Ministry of Science, ICT and Future Planning; . Ministry of Health & Welfare; . Catholic Kwandong University International St. Mary's Hospital.
Resumo: BACKGROUND: Osteoarthritis (OA) can be defined as degradation of articular cartilage of the joint, and is the most common degenerative disease. To regenerate the damaged cartilage, different experimental approaches including stem cell therapy have been tried. One of the major limitations of stem cell therapy is the poor post-transplantation survival of the stem cells. Anoikis, where insufficient matrix support and adhesion to extracellular matrix causes apoptotic cell death, is one of the main causes of the low post-transplantation survival rate of stem cells. Therefore, enhancing the initial interaction of the transplanted stem cells with chondrocytes could improve the therapeutic efficacy of stem cell therapy for OA. Previously, protein kinase C activator phorbol 12-myristate 13-acetate (PMA)- induced increase of mesenchymal stem cell adhesion via activation of focal adhesion kinase (FAK) has been reported. In the present study, we examine the effect PMA on the adipose-derived stem cells (ADSCs) adhesion and spreading to culture substrates, and further on the initial interaction between ADSC and chondrocytes. RESULTS: PMA treatment increased the initial adhesion of ADSC to culture substrate and cellular spreading with increased expression of adhesion molecules, such as FAK, vinculin, talin, and paxillin, at both RNA and protein level. Priming of ADSC with PMA increased the number of ADSCs attached to confluent layer of cultured chondrocytes compared to that of untreated ADSCs at early time point (4 h after seeding). CONCLUSION: Taken together, the results of this study suggest that priming ADSCs with PMA can increase the initial interaction with chondrocytes, and this proof of concept can be used to develop a non-invasive therapeutic approach for treating OA. It may also accelerate the regeneration process so that it can relieve the accompanied pain faster in OA patients. Further in vivo studies examining the therapeutic effect of PMA pretreatment of ADSCs for articular cartilage damage are required.
Descritores: Células-Tronco/efeitos dos fármacos
Proteína Quinase C/farmacologia
Cartilagem Articular/citologia
Condrócitos/citologia
-Adesão Celular
Comunicação Celular
Diferenciação Celular
Sobrevivência Celular
Western Blotting
Técnicas de Cultura de Células
Condrócitos/efeitos dos fármacos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central



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