Base de dados : LILACS
Pesquisa : G04.144 [Categoria DeCS]
Referências encontradas : 202 [refinar]
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  1 / 202 LILACS  
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Id: biblio-1283597
Autor: Velázquez-Hernández, María Elena; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E.
Título: Defensin c-thionin from Capsicum chinense improves butyrate cytotoxicity on human colon adenocarcinoma cell line Caco-2
Fonte: Electron. j. biotechnol;52:76-84, July. 2021. graf, ilus.
Idioma: en.
Projeto: CONACyT; . CIC14.5 to JELM; ICGEB.
Resumo: BACKGROUND: Butyrate is a histone deacetylase inhibitor that induces apoptosis and inhibits cell proliferation of colorectal cancer cells. To improve its anticancer activity, butyrate has been evaluated mixed with drugs and different molecules. Plant antimicrobial peptides are attractive anticancer alternative molecules because they show selective cytotoxic activity against different cancer cell lines. In this work, we explore if the plant defensin c-thionin (Capsicum chinense) can improve butyrate activity on Caco-2 cell line and we also determined the mechanism of death activated. RESULTS: The combined treatment of c-thionin (3.5 mM) and butyrate (50 mM) showed higher cytotoxicity on Caco-2 cells with respect to single treatments. Also, the combined treatment reduced cell proliferation and exhibited a higher rate of apoptosis than single treatments. Combined treatment induced caspases 8 and 9 activation to an extent comparable with that of butyrate while c-thionin did not activate caspases. Additionally, reactive oxygen species generation preceded the onset of apoptosis, and superoxide anion production was higher in cells treated with the combined treatment. CONCLUSIONS: The c-thionin from Habanero chili pepper improved the butyrate cytotoxicity on Caco-2 cells. This effect occurred through apoptosis induction associated with reactive oxygen species production. Therefore, the combination of butyrate with cytotoxic antimicrobial peptides could be an attractive strategy for cancer therapy.
Descritores: Butiratos
Capsicum/química
Adenocarcinoma
Neoplasias do Colo
-Ciclo Celular
Espécies Reativas de Oxigênio
Apoptose
Células CACO-2
Defensinas
Tioninas
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


  2 / 202 LILACS  
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Id: biblio-950760
Autor: Ruz, Gonzalo A; Timmermann, Tania; Barrera, Javiera; Goles, Eric.
Título: Neutral space analysis for a Boolean network model of the fission yeast cell cycle network
Fonte: Biol. Res;47:1-12, 2014. ilus, graf, tab.
Idioma: en.
Projeto: CONICYT-Chile; . ANILLO.
Resumo: BACKGROUND: Interactions between genes and their products give rise to complex circuits known as gene regulatory networks (GRN) that enable cells to process information and respond to external stimuli. Several important processes for life, depend of an accurate and context-specific regulation of gene expression, such as the cell cycle, which can be analyzed through its GRN, where deregulation can lead to cancer in animals or a directed regulation could be applied for biotechnological processes using yeast. An approach to study the robustness of GRN is through the neutral space. In this paper, we explore the neutral space of a Schizosaccharomyces pombe (fission yeast) cell cycle network through an evolution strategy to generate a neutral graph, composed of Boolean regulatory networks that share the same state sequences of the fission yeast cell cycle. RESULTS: Through simulations it was found that in the generated neutral graph, the functional networks that are not in the wildtype connected component have in general a Hamming distance more than 3 with the wildtype, and more than 10 between the other disconnected functional networks. Significant differences were found between the functional networks in the connected component of the wildtype network and the rest of the network, not only at a topological level, but also at the state space level, where significant differences in the distribution of the basin of attraction for the G1 fixed point was found for deterministic updating schemes. CONCLUSIONS: In general, functional networks in the wildtype network connected component, can mutate up to no more than 3 times, then they reach a point of no return where the networks leave the connected component of the wildtype. The proposed method to construct a neutral graph is general and can be used to explore the neutral space of other biologically interesting networks, and also formulate new biological hypotheses studying the functional networks in the wildtype network connected component.
Descritores: Schizosaccharomyces/fisiologia
Ciclo Celular/fisiologia
Quinases Ciclina-Dependentes/metabolismo
Redes Reguladoras de Genes/fisiologia
Modelos Biológicos
-Schizosaccharomyces/genética
Gráficos por Computador
Simulação por Computador
Fase G1/fisiologia
Redes Neurais de Computação
Proteínas de Ciclo Celular/metabolismo
Biologia Computacional
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  3 / 202 LILACS  
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Id: biblio-950791
Autor: Shen, Wenlong; Wang, Dong; Ye, Bingyu; Shi, Minglei; Zhang, Yan; Zhao, Zhihu.
Título: A possible role of Drosophila CTCF in mitotic bookmarking and maintaining chromatin domains during the cell cycle
Fonte: Biol. Res;48:1-8, 2015. graf.
Idioma: en.
Projeto: National Basic Research Program of China; . National Natural Science Foundation of China; . National Key Technology R& D Program of China.
Resumo: BACKGROUND: The CCCTC-binding factor (CTCF) is a highly conserved insulator protein that plays various roles in many cellular processes. CTCF is one of the main architecture proteins in higher eukaryotes, and in combination with other architecture proteins and regulators, also shapes the three-dimensional organization of a genome. Experiments show CTCF partially remains associated with chromatin during mitosis. However, the role of CTCF in the maintenance and propagation of genome architectures throughout the cell cycle remains elusive. RESULTS: We performed a comprehensive bioinformatics analysis on public datasets of Drosophila CTCF (dCTCF). We characterized dCTCF-binding sites according to their occupancy status during the cell cycle, and identified three classes: interphase-mitosis-common (IM), interphase-only (IO) and mitosis-only (MO) sites. Integrated function analysis showed dCTCF-binding sites of different classes might be involved in different biological processes, and IM sites were more conserved and more intensely bound. dCTCF-binding sites of the same class preferentially localized closer to each other, and were highly enriched at chromatin syntenic and topologically associating domains boundaries. CONCLUSIONS: Our results revealed different functions of dCTCF during the cell cycle and suggested that dCTCF might contribute to the establishment of the three-dimensional architecture of the Drosophila genome by maintaining local chromatin compartments throughout the whole cell cycle.
Descritores: Proteínas Repressoras/fisiologia
Cromatina/fisiologia
Proteínas de Drosophila/fisiologia
Drosophila melanogaster/química
Genoma de Inseto/genética
Mitose/fisiologia
-Sítios de Ligação
Sequência de Bases
Ciclo Celular/fisiologia
Sequência Conservada
Biologia Computacional
Sintenia
Montagem e Desmontagem da Cromatina/fisiologia
Anotação de Sequência Molecular
Conjuntos de Dados como Assunto
Fator de Ligação a CCCTC
Interfase/fisiologia
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  4 / 202 LILACS  
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Id: biblio-896296
Autor: Cheng, Yi; Gao, Yang; Zhao, Lu; Gao, Shunqiang; Zhang, Guoqiang; Zhang, Yan.
Título: Knockout of p16INK4a promotes aggregative growth of dermal papilla cells
Fonte: Rev. Assoc. Med. Bras. (1992);63(10):883-889, Oct. 2017. tab, graf.
Idioma: en.
Resumo: Summary Objective: Dermal papilla cells (DPCs) are located in the hair follicles and play an important role in hair growth. These cells have the ability to induce hair follicle formation when they display aggregative behavior. DPCs derived from the androgenetic alopecia (AGA) area undergo premature senescence in vitro, associated with p16INK4a expression. The aim of the current study was to investigate the expression of p16INK4a in aggregative and non-aggregative DPCs and the effect of p16INK4a down-regulation in these cells by adenovirus-mediated RNA interference (RNAi). Method: DPCs were isolated and cultured from healthy human scalp. p16INK4a gene and protein were detected in aggregative and non-aggregative cells. Expression of p16INK4a in DPCs was silenced by infection with rAd5-CDKN1A-1p2shRNA. Cell fate was monitored after infection. The growth of cells was measured by MTT assay. Cell cycle was evaluated by flow cytometry (FCM). Results: DPCs were isolated by digestion and showed aggregative behavior for six passages. The expression of p16INK4a showed a clear upward trend in non-aggregative cells when compared with aggregative group. p16INK4a expression was silenced by rAd5-CDKN1A-1p2shRNA (p<0.05). The p16INK4a-silenced cells grew more rapidly and exhibited a trend towards aggregative growth. There was an increase in the proportion of cells in G1 phase, while those in S phase were reduced after p16INK4a gene silencing (p<0.05). Conclusion: Our results suggest that p16INK4a plays an important role in the premature senescence and aggregative behavior of DPCs. These observations can lead to novel therapeutic strategies for treatment of AGA.
Descritores: Couro Cabeludo/citologia
Folículo Piloso/citologia
Genes p16/fisiologia
-Valores de Referência
Fatores de Tempo
Imuno-Histoquímica
Transfecção
Agregação Celular/genética
Ciclo Celular/genética
Células Cultivadas
Senescência Celular/genética
Derme/citologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proliferação de Células/genética
Alopecia/genética
Técnicas de Inativação de Genes/métodos
Citometria de Fluxo
Limites: Humanos
Masculino
Responsável: BR1.1 - BIREME


  5 / 202 LILACS  
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Id: biblio-1179932
Autor: Costa, Felipe D'Almeida.
Título: Comparação entre os perfis de expressão de microRNAs em Linfomas de Hodgkin Clássico / Comparison between microRNA expression profiles in Classic Hodgkin Lymphoma.
Fonte: São Paulo; s.n; 2019. 105 p. ilust, tabelas.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: Introdução: Linfoma de Hodgkin (LH) é um dos linfomas mais frequentes no mundo ocidental, com cerca de 3 a 4 novos casos a cada 100 mil pessoas. Os microRNAs são pequenas moléculas que regulam a transcrição gênica e estão associadas ao desenvolvimento e progressão das neoplasias. Em LH clássico (LHC), alguns microRNAs já foram identificados a partir de células infectadas pelo EBV. Além destes, outros microRNAs já foram descritos em LHC, tanto em estudos onde foram realizadas esta avaliação em culturas celulares como utilizando a massa tumoral total de linfonodos, mas com pouca correlação com o comportamento biológico. Objetivo: O presente estudo tem como objetivo principal avaliar o perfil de expressão de microRNA em LHC, comparando casos livres de doença após a terapia com casos que exibiram recidivas. Material e métodos: Foram avaliados 106 pacientes com o diagnóstico de LHC, sendo coletados dados clínicos e avaliados os marcadores imuno-histoquímicos CD30, CD15, CD20 e CD15 destes casos, além da presença de EBV por CISH. Vinte casos foram selecionados para a avaliação da expressão de 377 microRNAs, 10 casos com recidiva e 10 casos sem evidência de doença. Como grupos comparadores, foram selecionadas 8 amostras de linfonodos reativos, 4 com hiperplasia folicular e 4 com hiperplasia paracortical. Os resultados do perfil de expressão foram submetidos ao método de agrupamento hierárquico não supervisionado. Resultados e discussão: Os parâmetros clínico-patológicos desta coorte não diferiram daqueles encontrados na literatura. A sobrevida global e a sobrevida livre de progressão foram de 92,1% e 83,6% em 5 anos, respectivamente. Os fatores associados ao prognóstico em análise multivariada foram idade, níveis séricos de albumina e DHL e estadiamento. O perfil de expressão dos microRNAs de todos os casos de LHC possibilitaram a separação completa dos mesmos em relação aos linfonodos reativos, independentemente do tipo de hiperplasia. Na comparação entre os casos de LHC, foram identificados 3 microRNAs diferencialmente expressos: miR-502-3p e miR-363, ambos hipoexpressos no grupo que continha todos os casos com recidivas e miR-886-5p, hiperexpresso neste grupo. Os dois primeiros microRNAs estão associados a supressão tumoral, através da inibição da proliferação e migração celular. O miR-886-5p é associado ao aumento da proliferação celular e inibição da apoptose. Em linfomas, este já foi descrito como estando hiperexpresso em linfomas T, incluindo linfoma anaplásico de grandes células. Conclusão: Os LHC possuem um perfil distinto de expressão de microRNAs, com muitos deles envolvendo mecanismos fundamentais na oncogênese, como ciclo celular e apoptose. Os microRNAs encontrados diferencialmente expressos nos casos recidivados no presente estudo podem estar associados ao comportamento menos indolente destas neoplasias, podendo ser alvo de futuras investigações, incluindo seu uso como potenciais alvos de terapia

Introduction: Hodgkin lymphoma (HL) is one of the most common lymphomas in the western world, with about 3 to 4 new cases per 100,000 people. MicroRNAs are small molecules that regulate gene transcription and are associated with the development and progression of neoplasms. In classic HL (CHL), some microRNAs have already been identified from EBV-infected cells. In addition, other microRNAs have already been described in CHL, both in studies where this evaluation was performed in cell cultures and using whole lymph node tumor tissue, but with little correlation with biological behavior. Objective: The present study aims to evaluate the microRNA expression profile in CHL, comparing disease-free cases after therapy with cases that showed recurrences. A total of 106 patients diagnosed with CHL were evaluated. Clinical data were collected and the immunohistochemical markers CD30, CD15, CD20 and CD15 of these cases were evaluated, as well as the presence of EBV by CISH. Twenty cases were selected for the analysis of the expression of 377 microRNAs, 10 cases with recurrences and 10 cases without evidence of disease. For comparison, 8 samples of reactive lymph nodes were selected, 4 with follicular hyperplasia and 4 with paracortical hyperplasia. The expression profile results were submitted to the unsupervised hierarchical clustering method. Results and discussion: The clinico-pathological parameters of this cohort did not differ from those found in the literature. Overall survival and progression-free survival were 92.1% and 83.6% at 5 years, respectively. Factors associated with prognosis in multivariate analysis were age, serum albumin and DHL levels, and staging. The microRNA expression profile of all CHL cases allowed their complete separation from reactive lymph nodes, regardless of the type of hyperplasia. When comparing the cases of CHL, 3 differentially expressed microRNAs were identified: miR-502-3p and miR-363, both hypoexpressed in the group containing all relapsed cases and miR-886-5p, hyperexpressed in this group. The first two microRNAs are associated with tumor suppression through inhibition of cell proliferation and migration. The miR-886-5p is associated with increased cell proliferation and inhibition of apoptosis. In lymphomas, it has been described as being overexpressed in T-cell lymphomas, including anaplastic large cell lymphoma. Conclusion: CHLs have a distinct microRNA expression profile, with many involving key mechanisms in oncogenesis, such as cell cycle and apoptosis. The differentially expressed microRNAs found in the relapsed cases in the present study may be associated with the less indolent behavior of these neoplasms and may be the subject of future investigations, including their use as potential therapeutic targets
Descritores: Transcrição Genética
Doença de Hodgkin
Imuno-Histoquímica
Perfilação da Expressão Gênica
MicroRNAs
Linfoma
-Recidiva
Ciclo Celular
Apoptose
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: BR30.1 - Biblioteca
BR30.1


  6 / 202 LILACS  
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Id: biblio-950852
Autor: Wang, Min; Yan, Jingjun; He, Xingxing; Zhong, Qiang; Zhan, Chengye; Li, Shusheng.
Título: Candidate genes and pathogenesis investigation for sepsis-related acute respiratory distress syndrome based on gene expression profile
Fonte: Biol. Res;49:1-9, 2016. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Acute respiratory distress syndrome (ARDS) is a potentially devastating form of acute inflammatory lung injury as well as a major cause of acute respiratory failure. Although researchers have made significant progresses in elucidating the pathophysiology of this complex syndrome over the years, the absence of a universal detail disease mechanism up until now has led to a series of practical problems for a definitive treatment. This study aimed to predict some genes or pathways associated with sepsis-related ARDS based on a public microarray dataset and to further explore the molecular mechanism of ARDS. RESULTS: A total of 122 up-regulated DEGs and 91 down-regulated differentially expressed genes (DEGs) were obtained. The up- and down-regulated DEGs were mainly involved in functions like mitotic cell cycle and pathway like cell cycle. Protein-protein interaction network of ARDS analysis revealed 20 hub genes including cyclin B1 (CCNB1), cyclin B2 (CCNB2) and topoisomerase II alpha (TOP2A). A total of seven transcription factors including forkhead box protein M1 (FOXM1) and 30 target genes were revealed in the transcription factor-target gene regulation network. Furthermore, co-cited genes including CCNB2-CCNB1 were revealed in literature mining for the relations ARDS related genes. CONCLUSIONS: Pathways like mitotic cell cycle were closed related with the development of ARDS. Genes including CCNB1, CCNB2 and TOP2A, as well as transcription factors like FOXM1 might be used as the novel gene therapy targets for sepsis related ARDS
Descritores: Transtornos Respiratórios/genética
Sepse/complicações
Sepse/genética
Estudos de Associação Genética
Transcriptoma
-Fatores de Transcrição
Regulação para Baixo
Ciclo Celular/genética
Regulação para Cima
Marcação de Genes
Perfilação da Expressão Gênica
Bases de Dados Genéticas
Mapas de Interação de Proteínas
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


  7 / 202 LILACS  
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Id: biblio-950868
Autor: Wang, Hui; Liu, Zheng; Zhang, Wenxiu; Yuan, Ziao; Yuan, Hongyi; Liu, Xueting; Yang, Chunwen; Guan, Weijun.
Título: Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis
Fonte: Biol. Res;49:1-14, 2016. ilus, graf.
Idioma: en.
Projeto: Natural Science Foundation of Liaoning Province; . Agricultural Science and Technology Innovation Program.
Resumo: BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 µM) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It also increased K+ efflux and altered the mRNA levels of Bax, Bcl-2, caspase-3, caspase-8, Fas, and p53. CONCLUSIONS: Our results suggest that Cd2+ triggers the apoptosis of Siberian tiger fibroblasts by disturbing intracellular homeostasis. These results will aid in our understanding of the effects of Cd2+ on Siberian tigers and in developing interventions to treat and prevent cadmium poisoning.
Descritores: Cádmio/toxicidade
Apoptose/efeitos dos fármacos
Espaço Intracelular/efeitos dos fármacos
Tigres
Fibroblastos/efeitos dos fármacos
Homeostase/efeitos dos fármacos
-Sibéria
Dano ao DNA
Ciclo Celular/efeitos dos fármacos
Células Cultivadas
Reação em Cadeia da Polimerase
Espécies Reativas de Oxigênio/análise
Apoptose/genética
Caspases/análise
Caspases/efeitos dos fármacos
Ensaio Cometa/veterinária
Microscopia Eletrônica de Transmissão
Transcrição Reversa
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Fibroblastos/fisiologia
Homeostase/fisiologia
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950869
Autor: Visagie, Michelle Helen; Jaiswal, Seema Rummurat; Joubert, Anna Margaretha.
Título: In vitro assessment of a computer-designed potential anticancer agent in cervical cancer cells
Fonte: Biol. Res;49:1-13, 2016. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Computer-based technology is becoming increasingly essential in biological research where drug discovery programs start with the identification of suitable drug targets. 2-Methoxyestradiol (2ME2) is a 17ß-estradiol metabolite that induces apoptosis in various cancer cell lines including cervical cancer, breast cancer and multiple myeloma. Owing to 2ME2's poor in vivo bioavailability, our laboratory in silico-designed and subsequently synthesized a novel 2ME2 analogue, 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10),15-tetraen-17-ol (ESE-15-ol), using receptor- and ligand molecular modeling. In this study, the biological effects of ESE-15-ol (180 nM) and its parent molecule, 2ME2 (1 µM), were assessed on morphology and apoptosis induction in cervical cancer cells. RESULTS: Transmission electron microscopy, scanning electron microscopy and polarization-optical transmitted light differential interference contrast (PlasDIC) images demonstrated morphological hallmarks of apoptosis including apoptotic bodies, shrunken cells, vacuoles, reduced cell density and cell debris. Flow cytometry analysis showed apoptosis induction by means of annexin V-FITC staining. Cell cycle analysis showed that ESE-15-ol exposure resulted in a statistically significant increase in the G2M phase (72%) compared to 2ME2 (19%). Apoptosis induction was more pronounced when cells were exposed to ESE-15-ol compared to 2ME2. Spectrophotometric analysis of caspase 8 activity demonstrated that 2ME2 and ESE-15-ol both induced caspase 8 activation by 2- and 1.7-fold respectively indicating the induction of the apoptosis. However, ESE-15-ol exerted all of the above-mentioned effects at a much lower pharmacological concentration (180 nM) compared to 2ME2 (1 µM physiological concentration). CONCLUSION: Computer-based technology is essential in drug discovery and together with in vitro studies for the evaluation of these in silico-designed compounds, drug development can be improved to be cost effective and time consuming. This study evaluated the anticancer potential of ESE-15-ol, an in silico-designed compound in vitro. Research demonstrated that ESE-15-ol exerts antiproliferative activity accompanied with apoptosis induction at a nanomolar concentration compared to the micromolar range required by 2ME2. This study is the first study to demonstrate the influence of ESE-15-ol on morphology, cell cycle progression and apoptosis induction in HeLa cells. In silico-design by means of receptor- and ligand molecular modeling is thus effective in improving compound bioavailability while preserving apoptotic activity in vitro.
Descritores: Sulfonamidas/farmacologia
Inibidores da Anidrase Carbônica/farmacologia
Neoplasias do Colo do Útero/tratamento farmacológico
Desenho Assistido por Computador
Estradiol/análogos & derivados
Antineoplásicos/farmacologia
-Fatores de Tempo
Células HeLa
Microscopia Eletrônica de Varredura
Ciclo Celular/efeitos dos fármacos
Ciclo Celular/fisiologia
Células Cultivadas
Neoplasias do Colo do Útero/patologia
Reprodutibilidade dos Testes
Apoptose/efeitos dos fármacos
Meios de Cultura
Microscopia Eletrônica de Transmissão
Estradiol/farmacologia
Caspase 8/metabolismo
Citometria de Fluxo/métodos
2-Metoxiestradiol
Microscopia de Polarização
Limites: Humanos
Feminino
Tipo de Publ: Estudo de Avaliação
Responsável: CL1.1 - Biblioteca Central


  9 / 202 LILACS  
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Id: biblio-870261
Autor: Silva, Carlos Augusto Moreira.
Título: Caracterização da expressão proteica por imuno-histoquímica de genes relacionados no cromossomo 9P em linfoma de grandes células b primáriodo mediastino e sua relação com marcadores de proliferação e ciclo celular / Characterization of protein expression by immunohistochemistry of genes related to changes in chromosome 9p in primary mediastinal large Bcell lymphoma and its relationship with proliferation markers and cell cycle.
Fonte: São Paulo; s.n; 2015. 89 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: O linfoma de grandes células B primário mediastinal tímico (LBPM) possui alterações genéticas particulares, dentre as quais, amplificação da região cromossômica 9p. Recentes estudos correlacionam à patogênese do LBPM alguns genes (JMJD2c, SOCS1, STAT6, JAK2 e PDL1) associados direta ou indiretamente a esta região cromossômica. Estes são responsáveis por ativarem ou bloquearem diversas vias de sinalização que promovem aumento da atividade proliferativa celular e/ou escape a mecanismos imunes de defesa do hospedeiro, originando fenômenos oncogênicos. O objetivo deste estudo foi avaliar a expressão proteica por imuno-histoquímica de genes relacionados a alterações cromossômica no 9p e correlacioná-las à expressão de marcadores relacionados à proliferação e ciclo celular, bem como a parâmetros clínicos e evolutivos. Para tanto, avaliamos 63 pacientes procedentes de 5 instituições do Estado de São Paulo diagnosticados no período de 1975 a 2011. Expressão de JAK2 (p=0,0009), PDL1 (p=0,0007) e p16 (p=0,0058) bem como estádio clínico agrupado (p=0,0015) e IPI (p<0,0001), foram fatores prognósticos relacionados a melhor sobrevida global...

Thymic primary mediastinal large B-cell lymphoma (LBPM) has particular genetic changes, among which, amplification of chromosome 9p region. Recent studies correlate some genes (JMJD2c, SOCS1, STAT6, JAK2 and PDL1) directly or indirectly related to this chromosome region to the pathogenesis of LBPM. These are responsible for activating or blocking several signaling pathways that result in increased cell proliferative activity and / or escape the immune defense mechanisms of the host, leading to oncogenic phenomena. The objective of this study was to evaluate the protein expression by immunohistochemistry of genes related to chromosomal alterations in 9p and correlates them the expression of markers related to proliferation and cell cycle as well as clinical and outcome parameters. Therefore, we evaluated 63 patients coming from 5 institutions of the State of São Paulo diagnosed from 1975 to 2011. Expression JAK2 (p = 0.0009), PDL1 (p = 0.0007) and p16 (p = 0.0058 ) grouped clinical stage (p = 0.0015) and IPI (p <0.0001) were prognostic factors related to better overall survival...
Descritores: Cromossomos Humanos Par 9
Imuno-Histoquímica
Linfoma
Mediastino
Prognóstico
Sobrevida
-Ciclo Celular
Proliferação de Células
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Guia
Responsável: BR30.1 - Biblioteca
BR30.1


  10 / 202 LILACS  
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Id: biblio-961283
Autor: Sierra Benítez, Enrique Marcos; León Pérez, Mairianny Quianella; Laud Rodríguez, Lenier; Carrillo Comas, Alberto Lázaro; Pérez Ortiz, Letier; Rodríguez Ramos, Eglys.
Título: Gliomas malignos: biología molecular y detalles oncogenéticos / Wicked gliomas: molecular biology and oncogenetics detail
Fonte: Rev. medica electron;40(4):1100-1111, jul.-ago. 2018. ilus.
Idioma: es.
Resumo: RESUMEN La biología de los gliomas malignos se asocia con el balance de la expresión de las proteínas que controlan de manera positiva o negativa el ciclo celular, la proliferación, la motilidad, la neoformación vascular y el reconocimiento del sistema inmune. La frecuencia de las alteraciones genéticas que están presentes en GBM2 y GBM1 son diferentes así como la edad de los pacientes en la que se presentan. Mientras que los GBM1 suelen aparecer en edades más tardías, alrededor de los 60-70 años, los GBM2 suelen presentarse en edades más tempranas, 40-50 años. En la génesis del glioblastoma existen alteraciones moleculares a nivel de genes supresores de tumores, oncogenes y genes reparadores de ADN (AU).

ABSTRACT The glioblastoma it is the primary wicked tumor of the central nervous system more common in adults and it invariably associates to a bad presage. The biology of the wicked gliomas associates with the balance of the expression of the proteins that they control of positive way or negative the cellular cycle, the proliferation, the motility, the vascular neoformation and the recognition of the immune system. The frequency of the genetic alterations that they are present in GBM2 and GBM1 is different. While the GBM1 usually appears in later ages, around the 60-70 years, the GBM2 usually presents in earlier ages, 40-50 years. In the genesis of the glioblastoma exist molecular alterations at level of suppressive genes of tumors (GST), oncogenes and reparative genes of DNA (AU).
Descritores: Oncogenes/genética
Biologia/classificação
DNA/classificação
-Pacientes
Proteínas
Ciclo Celular
Genes Supressores
Glioblastoma
Genes/genética
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CU424.1 - Centro Provincial de Información de Ciencias Médicas



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