Base de dados : LILACS
Pesquisa : G04.146 [Categoria DeCS]
Referências encontradas : 219 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 22 ir para página                         

  1 / 219 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Mantovani, Mário Sérgio
Texto completo
Texto completo
Id: biblio-1283485
Autor: Biazi, Bruna Isabela; Zanetti, Thalita Alves; Marques, Lilian Areal; Baranoski, Adrivanio; Coatti, Giuliana Castello; Mantovani, Mário Sérgio.
Título: mRNAs biomarker related to the control of proliferation and cell death in HepG2/C3A spheroid and monolayer cultures treated with piperlongumine
Fonte: Appl. cancer res;40:1-13, Oct. 19, 2020. ilus.
Idioma: en.
Resumo: Background: Cell culture (spheroid and 2D monolayer cultures) is an essential tool in drug discovery. Piperlongumine (PLN), a naturally occurring alkaloid present in the long pepper (Piper longum), has been implicated in the regulation of GSTP1 activity. In vitro treatment of cancer cells with PLN increases ROS (reactive oxygen species) levels and induces cell death, but its molecular mode of action has not been entirely elucidated. Methods: In this study, we correlated the antiproliferative effects (2D and 3D cultures) of PLN (CAS 20069­09-4, Sigma-Aldrich) with morphological and molecular analyses in HepG2/C3A cell line. We performed assays for cytotoxicity (MTT), comet assays for genotoxicity, induction of apoptosis, analysis of the cell cycle phase, and analysis of the membrane integrity by flow cytometry. Relative expression of mRNA of genes related to proliferation, apoptosis, cell cycle control, metabolism of xenobiotics, and reticulum endoplasmic stress. Results: PLN reduced the cell proliferation by the cell cycle arrest in G2/M. Changes in the mRNA expression for CDKN1A (4.9x) and CCNA2 (0.5x) of cell cycle control genes were observed. Cell death occurred due to apoptosis, which may have been induced by increased expression of proapoptotic mRNAs (BAK1, 3.1x; BBC3, 2.4x), and by an increase in 9 and 3/7 active caspases. PLN induced cellular injury by ROS generation and DNA damage. DNA damage induced MDM2 signaling (3.0x) associated with the appearance of the monastral spindle in mitosis. Genes associated with ROS degradation also showed increased mRNA expression (GSR, 2.0x; SOD1, 2.1x). PLN induce endoplasmic reticulum stress with the increase in the mRNA expression of ERN1 (4.5x) and HSPA14 (2.2x). The xenobiotic metabolism showed increased mRNA expression for CYP1A2 (2.2x) and CYP3A4 (3.4x). In addition to 2D culture, PLN treatment also inhibited the growth of 3D culture (spheroids). Conclusion: Thus, the findings of our study show that several gene expression biomarkers (mRNAs) and monastral spindle formation indicated the many pathways of damage induced by PLN treatment that contributes to its antiproliferative effects
Descritores: RNA Mensageiro/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
Técnicas de Cultura de Células
Proliferação de Células/efeitos dos fármacos
Dioxolanos/farmacologia
Antineoplásicos/farmacologia
-Biomarcadores/análise
Expressão Gênica/efeitos dos fármacos
Esferoides Celulares/efeitos dos fármacos
Células Hep G2/efeitos dos fármacos
Limites: Humanos
Responsável: BR30.1 - Biblioteca


  2 / 219 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-753028
Autor: Gracitelli, Carolina Pelegrini Barbosa; Paranhos Junior, Augusto.
Título: Can glaucoma affect sleep quality? / O glaucoma pode afetar a qualidade do sono?
Fonte: Arq. bras. oftalmol;78(3):V-VI, May-Jun/2015.
Idioma: en.
Projeto: Brazilian National Research Council.
Descritores: Células Ganglionares da Retina
Glaucoma/complicações
Ritmo Circadiano/fisiologia
Morte Celular
Transtornos do Sono do Ritmo Circadiano/etiologia
-Reflexo Pupilar/fisiologia
Glaucoma/fisiopatologia
Transtornos do Sono do Ritmo Circadiano/fisiopatologia
Limites: Humanos
Responsável: BR1.1 - BIREME


  3 / 219 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-478282
Autor: Lima, Rafael Rodrigues; Costa, Ana Maria Rabelo; Souza, Renata Duarte de; Gomes-Leal, Walace.
Título: Degeneração neuronal secundária e excitotoxicidade / Secondary neurodegeneration and excitotoxicity
Fonte: Rev. para. med = Rev. Para. Med. (Impr.);21(1):27-31, jan.-mar. 2007.
Idioma: pt.
Resumo: Objetivo: caracterizar os principais eventos correlatos entre degeneração neuronal secundária e excitotoxicidade em doenças neurodegenerativas. Método: revisão de literatura através de pesquisa bibliográfica na base de dados PUBMED/MEDLINE. Considerações Finais: o dano tecidual proveniente de lesões isquêmicas ou traumáticas no sistema nervoso central não se restringe somente à lesão primária propriamente dita, mas também, e em grande intensidade do processo de degeneração neuronal secundária desencadeada, seja pela resposta inflamatória ou através de cascatas destrutivas ativadas durante a excitotoxicidade mediada por glutamato. O melhor entendimento molecular desse processo toma-se necessário para um bloqueio seletivo desses eventos patológicos, minimizando os efeitos colaterais, o que permitirá a elaboração de abordagens terapêuticas neuroprotetoras eficazes.

Objective: Review the main experimental evidences for a role of excitotoxic events on the pathophysiology neurodegenerative diseases. Methods: Literature review through bibliographical search in the databases PUBMED/MEDLINE. Final considerations: The tis sue damage occurring following ischemic and/or traumatic injuries in the central system nervous is not due the primary pathological events, but also, and more importantly, to the second neurodegeneration involving excitotoxicity, inflammation, oxidative stress, apoptosis. The understanding of t molecular mechanisms of these events is necessary for the more effective development of neuroprotectiveneuroregenerative therapies for CNS diseases.
Descritores: Degeneração Neural
Doenças Neurodegenerativas
Revisão
Ácido Glutâmico
-Morte Celular
Responsável: BR3.1 - Biblioteca Central


  4 / 219 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-952830
Autor: Strauss, Bryan E; Silva, Gissele Rolemberg Oliveira; de Luna Vieira, Igor; Cerqueira, Otto Luiz Dutra; Del Valle, Paulo Roberto; Medrano, Ruan Felipe Vieira; Mendonça, Samir Andrade.
Título: Perspectives for cancer immunotherapy mediated by p19Arf plus interferon-beta gene transfer
Fonte: Clinics;73(supl.1):e479s, 2018. graf.
Idioma: en.
Projeto: IVL; . MGL; . PRDV; . RFVM; . BES.
Resumo: While cancer immunotherapy has gained much deserved attention in recent years, many areas regarding the optimization of such modalities remain unexplored, including the development of novel approaches and the strategic combination of therapies that target multiple aspects of the cancer-immunity cycle. Our own work involves the use of gene transfer technology to promote cell death and immune stimulation. Such immunogenic cell death, mediated by the combined transfer of the alternate reading frame (p14ARF in humans and p19Arf in mice) and the interferon-β cDNA in our case, was shown to promote an antitumor immune response in mouse models of melanoma and lung carcinoma. With these encouraging results, we are now setting out on the road toward translational and preclinical development of our novel immunotherapeutic approach. Here, we outline the perspectives and challenges that we face, including the use of human tumor and immune cells to verify the response seen in mouse models and the incorporation of clinically relevant models, such as patient-derived xenografts and spontaneous tumors in animals. In addition, we seek to combine our immunotherapeutic approach with other treatments, such as chemotherapy or checkpoint blockade, with the goal of reducing dosage and increasing efficacy. The success of any translational research requires the cooperation of a multidisciplinary team of professionals involved in laboratory and clinical research, a relationship that is fostered at the Cancer Institute of Sao Paulo.
Descritores: Terapia Genética/métodos
Fases de Leitura/genética
Interferon beta/uso terapêutico
Técnicas de Transferência de Genes
Imunoterapia/métodos
Neoplasias/terapia
-Morte Celular/genética
Inibidor p16 de Quinase Dependente de Ciclina/genética
Proteína Supressora de Tumor p14ARF/genética
Neoplasias/imunologia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  5 / 219 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974950
Autor: Carvalho, Heloisa de Andrade; Villar, Rosangela Correa.
Título: Radiotherapy and immune response: the systemic effects of a local treatment
Fonte: Clinics;73(supl.1):e557s, 2018. tab, graf.
Idioma: en.
Resumo: Technological developments have allowed improvements in radiotherapy delivery, with higher precision and better sparing of normal tissue. For many years, it has been well known that ionizing radiation has not only local action but also systemic effects by triggering many molecular signaling pathways. There is still a lack of knowledge of this issue. This review focuses on the current literature about the effects of ionizing radiation on the immune system, either suppressing or stimulating the host reactions against the tumor, and the factors that interact with these responses, such as the radiation dose and dose / fraction effects in the tumor microenvironment and vasculature. In addition, some implications of these effects in cancer treatment, mainly in combined strategies, are addressed from the perspective of their interactions with the more advanced technology currently available, such as heavy ion therapy and nanotechnology.
Descritores: Radiação Ionizante
Radioterapia/efeitos adversos
Sistema Imunitário/efeitos da radiação
Neoplasias/imunologia
Neoplasias/radioterapia
-Radioterapia/tendências
Morte Celular/efeitos da radiação
Apoptose/efeitos da radiação
Apoptose/imunologia
Relação Dose-Resposta à Radiação
Imunoterapia/métodos
Imunoterapia/tendências
Necrose/etiologia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  6 / 219 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-950746
Autor: Bae, Seunghee; Lim, Kyung Mi; Cha, Hwa Jun; An, In-Sook; Lee, Jeong Pyo; Lee, Kwang Sik; Lee, Ghang Tai; Lee, Kun Kook; Jung, Ho Jung; Ahn, Kyu Joong; An, Sungkwan.
Título: Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
Fonte: Biol. Res;47:1-11, 2014. graf, tab.
Idioma: en.
Projeto: Korean Health Technology R& D Project; . Ministry of Health & Welfare, Republic of Korea.
Resumo: BACKGROUND: Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is also known alopecia. Arctiin is an active lignin isolated from Arctium lappa and has anti-inflammation, anti-microbial, and anti-carcinogenic effects. In the present study, we found that arctiin exerts anti-oxidative effects on human hair dermal papilla cells (HHDPCs). RESULTS: To better understand the mechanism, we analyzed the level of hydrogen peroxide (H2O2)-induced cytotoxicity, cell death, ROS production and senescence after arctiin pretreatment of HHDPCs. The results showed that arctiin pretreatment significantly inhibited the H2O2-induced reduction in cell viability. Moreover, H2O2-induced sub-G1 phase accumulation and G2 cell cycle arrest were also downregulated by arctiin pretreatment. Interestingly, the increase in intracellular ROS mediated by H2O2 was drastically decreased in HHDPCs cultured in the presence of arctiin. This effect was confirmed by senescence associated-beta galactosidase (SA-ß-gal) assay results; we found that arctiin pretreatment impaired H2O2-induced senescence in HHDPCs. Using microRNA (miRNA) microarray and bioinformatic analysis, we showed that this anti-oxidative effect of arctiin in HHDPCs was related with mitogen-activated protein kinase (MAPK) and Wnt signaling pathways. CONCLUSIONS: Taken together, our data suggest that arctiin has a protective effect on ROS-induced cell dysfunction in HHDPCs and may therefore be useful for alopecia prevention and treatment strategies.
Descritores: Envelhecimento/metabolismo
Espécies Reativas de Oxigênio/antagonistas & inibidores
Folículo Piloso/efeitos dos fármacos
MicroRNAs/metabolismo
Furanos/farmacologia
Glucosídeos/farmacologia
-Envelhecimento/efeitos dos fármacos
Regulação para Baixo/efeitos dos fármacos
Regulação para Cima/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
beta-Galactosidase/análise
Folículo Piloso/citologia
Folículo Piloso/metabolismo
Derme/citologia
Derme/efeitos dos fármacos
Derme/metabolismo
Análise de Sequência com Séries de Oligonucleotídeos
MicroRNAs/efeitos dos fármacos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Peróxido de Hidrogênio/farmacologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  7 / 219 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1132180
Autor: Ribeiro, Iára Mariana Léllis; Pereira, Wagner Luiz; Nogueira, Leonardo Brandão; Oliveira, Laser Antônio Machado; Teixeira, Róbson Ricardo; Nogueira, Katiane de Oliveira Pinto Coelho.
Título: Neuroprotective Effect of Isobenzofuranones on Hydrogen Peroxide-Mediated Redox Imbalance in Primary Cultures of Hippocampal Neurons
Fonte: Braz. arch. biol. technol;63:e20190072, 2020. graf.
Idioma: en.
Projeto: Minas Gerais Funding Foundation.
Resumo: Abstract In live organisms, there is a balance between the production of reactive oxygen species (ROS) and their neutralization. The increased level of these species leads to a condition called redox imbalance. The aim of this study was to evaluate the protective action of isobenzofuranones in primary cultures of hippocampal neurons subjected to redox imbalance. To accomplish this, MTT and LIVE/DEAD assays were initially performed. In the cultures pretreated with isobenzofuranones 1 and 2, there was a higher number of live cells when compared to that in the untreated ones. Regarding redox imbalance, there was a significant increase in the intracellular levels of ROS. The cultures pretreated with isobenzofuranones showed a reduction in ROS levels. Lipid peroxidation caused by oxidative damage was significantly reduced in the cultures pretreated with isobenzofuranones 1 and 2. Taken together, these data show the ability of isobenzofuranones 1 and 2 to significantly minimize cytotoxicity, cell death, intracellular levels of ROS and lipid peroxidation induced by redox imbalance. These results suggest that isobenzofuranones 1 and 2 represent a possible alternative therapy for the neurodegenerative disturbances that are triggered by ROS production increases.
Descritores: Oxirredução/efeitos dos fármacos
Benzofuranos/farmacologia
Espécies Reativas de Oxigênio
Fármacos Neuroprotetores/farmacologia
Peróxido de Hidrogênio
-Benzofuranos/síntese química
Morte Celular
Cultura Primária de Células
Hipocampo/citologia
Neurônios/metabolismo
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


  8 / 219 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-774689
Autor: Gomes, Marleide da Mota.
Título: The opportunity to remember William Richard Gowers (1845-1915) and his concept of abiotrophy, now best understood and called apoptosis or programmed cell death / A oportunidade de rememorar William Richard Gowers (1845-1915) e seu conceito de abiotrofia, agora melhor compreendido e denominado apoptose ou morte celular programada
Fonte: Rev. bras. neurol;51(4):106-109, out.-dez. 2015. ilus.
Idioma: en.
Resumo: William Richard Gowers (1845-1915) was one of the most prolific and insightful neurologists of all times. He gave major contributions to the development of the medicine through the presentation of signs, syndromes, new concepts and many publications, mainly based on his thorough examination of his patients at the National Hospital, London. In this paper, they were enrolled several Gowers' contribu-tions, besides, it is discussed his concept of abiotrophy. Gowers at his time already recognized what is now most deeply understood  and called programmed cell death or apoptosis that occurs normally du-ring the development or aging, but also under a range of stimuli and conditions, physiological or pathological, on the dependence of a cell selective vulnerability.

William Richard Gowers (1845-1915) foi um dos mais prolíficos e perspicazes neurologistas de todos os tempos. Ele deu grandes contribuições para o desenvolvimento da medicina, por meio da apresentação de sinais, síndromes, novos conceitos e muitas publicações, principalmente com base em sua análise aprofundada de seus pacientes no National Hospital, em Londres. Neste trabalho, foram registradas várias contribuições, além disso, discute-se o seu conceito de abiotrofia. Gowers, na sua época, já reconheceu o que agora é mais profundamente compreendido e denominado de morte celular programada ou apoptose, que ocorre normalmente durante o desenvolvimento ou envelhecimento, mas também sob uma gama de estímulos e condições, fisiológicas ou patológicas, na dependência de uma vulnerabilidade seletiva de células.
Descritores: Morte Celular
Apoptose
Abiotrophia
Neurologia/história
-Biografias como Assunto
Londres
Doenças do Sistema Nervoso/história
Limites: Humanos
História do Século XIX
História do Século XX
Responsável: BR14.1 - Biblioteca Central


  9 / 219 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
Id: biblio-964691
Autor: Ramos, Rogério Rodrigo; Paiva, Jeferson Leandro de; Boer, Nagib Pezati; Boer, Luis Fernando Ricci; Batigália, Fernando; Godoy, José Maria Pereira de.
Título: Evidências do efeito da terapia fotodinâmica sobre a Propionibacterium acnes / Evidence of the effect of photodynamic therapy on Propionibacterium acnes
Fonte: J. Health Biol. Sci. (Online);6(3):269-272, 02/07/2018. tab, graf.
Idioma: pt.
Resumo: Introdução: A Propionibacterium acnes é uma bactéria causadora da acne. Devido aos efeitos colaterais ou à falta de resposta ao tratamento da acne, foi proposta a terapia fotodinâmica como um tratamento alternativo para a acne. Objetivo: O objetivo foi evidenciar a ação fotodinâmica do LED vermelho 660 nm e do fotossensibilizador azul de metileno sobre Propionibacterium acnes in vitro. Métodos: Os ensaios foram constituídos por quatro grupos: 1. controle (sem aplicação de luz e sem fotossensibilizador); 2. com aplicação de luz; 3. com fotossensibilizador e sem aplicação de luz; 4. com fotossensibilizador e com aplicação de luz. Os ensaios foram submetidos a aplicação de luz por 4 ciclos de 5 minutos com intervalos de 3 minutos. Resultados: Houve redução estatisticamente significante (p<0,05) nas médias dos grupos 1, 2 e 4, ainda que o grupo 3 não tenha apresentado significância estatística, mas houve redução detectada nas médias. Conclusão: A ação fotodinâmica é eficiente para a destruição do material biológico por irradiação a 660nm atribuída ao processo de fotossensibilização pela presença do fotossensibilizador.(AU)

Introduction: Propionibacterium acnes is a bacterium that causes acne. Due to the side effects or the lack of response to acne treatment, photodynamic therapy was proposed as an alternative treatment for acne. Objective: To demonstrate the photodynamic action of the 660 nm red LED and the methylene blue photosensitizer on Propionibacterium acnes in vitro. Methods: Four groups were studied: 1. control (without light application and without photosensitizer); 2. with light application; 3. with photosensitizer and without light application; 4. with photosensitizer and light application. The assays were subjected to light application for 4 cycles of 5 minutes at 3 minute intervals. Results: There was a statistically significant reduction (p <0.05) in the means of groups 1, 2 and 4, although group 3 did not present statistical significance, but there was a reduction detected in the means. Conclusion: The photodynamic action is efficient for the destruction of the biological material by irradiation at 660nm attributed to the process of photosensitization by the presence of the photosensitizer.(AU)
Descritores: Fototerapia
Propionibacterium acnes
Fármacos Fotossensibilizantes
-Morte Celular
Oxigênio Singlete
Responsável: BR1780.2


  10 / 219 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-950847
Autor: Faisal, Mohammad; Saquib, Quaiser; Alatar, Abdulrahman A; Al-Khedhairy, Abdulaziz A; Ahmed, Mukhtar; Ansari, Sabiha M; Alwathnani, Hend A; Dwivedi, Sourabh; Musarrat, Javed; Praveen, Shelly.
Título: Cobalt oxide nanoparticles aggravate DNA damage and cell death in eggplant via mitochondrial swelling and NO signaling pathway
Fonte: Biol. Res;49:1-13, 2016. ilus, graf.
Idioma: en.
Projeto: National Plan for Science, Technology and Innovation; . King Abdul Aziz City for Science and Technology.
Resumo: BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron microscopy (TEM) demonstrated the uptake and translocation of Co3O4-NPs into the cytoplasm. Intracellular presence of Co3O4-NPs triggered subcellular changes such as degeneration of mitochondrial cristae, abundance of peroxisomes and excessive vacuolization. Flow cytometric analysis of Co3O4-NPs (1.0 mg/ml) treated root protoplasts revealed 157, 282 and 178 % increase in reactive oxygen species (ROS), membrane potential (APm) and nitric oxide (NO), respectively. Besides, the esterase activity in treated protoplasts was also found compromised. About 2.4-fold greater level of DNA damage, as compared to untreated control was observed in Comet assay, and 73.2 % of Co3O4-NPs treated cells appeared apoptotic in flow cytometry based cell cycle analysis. CONCLUSION: This study demonstrate the phytotoxic potential of Co3O4-NPs in terms of reduction in seed germination, root growth, greater level of DNA and mitochondrial damage, oxidative stress and cell death in eggplant. The data generated from this study will provide a strong background to draw attention on Co3O4-NPs environmental hazards to vegetable crops.
Descritores: Óxidos/toxicidade
Dano ao DNA/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
Cobalto/toxicidade
Solanum melongena/efeitos dos fármacos
Nanopartículas/toxicidade
Dilatação Mitocondrial/efeitos dos fármacos
Óxido Nítrico/metabolismo
-Óxidos/metabolismo
Análise de Variância
Espécies Reativas de Oxigênio/metabolismo
Cobalto/metabolismo
Ensaio Cometa
Solanum melongena/metabolismo
Microscopia Eletrônica de Transmissão
Nanopartículas/metabolismo
Citometria de Fluxo
Dilatação Mitocondrial/fisiologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central



página 1 de 22 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde