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Pesquisa : G04.152 [Categoria DeCS]
Referências encontradas : 396 [refinar]
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Plapler, Hélio
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Id: biblio-989058
Autor: Morsoleto, Maria Jose Misael da Silva; Sella, Valeria; Machado, Paula; Bomfim, Fernando do; Fernandes, Maria Helena; Morgado, Fernando; Lopes Filho, Gaspar de Jesus; Plapler, Helio.
Título: Effect of low power laser in biomodulation of cultured osteoblastic cells of Wistar rats
Fonte: Acta cir. bras;34(2):e201900210, 2019. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract Purpose: To analyze aspects of the biomodulating effect of light in biological tissues, bone cells from surgical explants of the femur of rats were irradiated with low intensity laser. Methods: Bone cells were cultured and irradiated with LASER light (GaAlAs). Growth, cell viability, mineralized matrix formation, total protein dosage, immunostimulatory properties, cytochemical analysis, gene expression of bone proteins were examined using live cell imaging and cell counting by colorimetric assay. The gene expression of: alkaline phosphatase (ALP), type 1 collagen, osteocalcin and osteopontin through the real-time polymerase chain reaction. Results: At 8 days, the viability of the irradiated culture was 82.3% and 72.4% in non-irradiated cells. At 18 days, the cellular viability (with laser) was 77.42% and 47.62% without laser. At 8 days, the total protein concentration was 21.622 mg / mol in the irradiated group and 16, 604 mg / mol in the non-irradiated group and at 18 days the concentration was 37.25 mg / mol in the irradiated group and 24, 95 mg / mol in the non-irradiated group. Conclusion: The laser interfered in the histochemical reaction, cell viability, matrix mineralization, and maintained the cellular expression of proteins
Descritores: Osteoblastos/efeitos da radiação
Diferenciação Celular/efeitos da radiação
Sobrevivência Celular/efeitos da radiação
Terapia com Luz de Baixa Intensidade/métodos
-Fatores de Tempo
Células Cultivadas
Ratos Wistar
Relação Dose-Resposta à Radiação
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-887148
Autor: Mansouri, Mahnaz; Mansouri, Parvine; Raze, Abbas Ali; Jadali, Zohreh.
Título: The potential role of Th17 lymphocytes in patients with psoriasis
Fonte: An. bras. dermatol;93(1):63-66, Jan.-Feb. 2018. tab, graf.
Idioma: en.
Resumo: Abstract: Background: Psoriasis is a chronic inflammatory disorder, characterized by increased keratinocyte proliferation due to abnormal differentiation of basal keratinocytes. The etiology of the disease is unclear, and according to the survey results, it is hypothesized that a combination of genetic and environmental factors prompts an abnormal immune response in patients with psoriasis. CD4+ Th cells play a multifaceted role in both immune defense and pathogenesis of certain diseases such as psoriasis. Nonetheless, the exact contribution of different subpopulations of Th cells in psoriasis is still not clear. Objective: The aim of present study was to determine the mRNA expression level of RORC as potential inducer of Th17 cell differentiation and expression pattern of Th17-signature cytokines (IL-17A and IL-22). Methods: Twenty patients with psoriasis and twenty-one healthy subjects were included in the study. Peripheral blood mononuclear cells (PBMCs) were separated and expression of three genes were determined by quantitative real-time reverse transcriptase PCR (qRT-PCR). Plasma levels of IL-17 and IL-22 were also evaluated by ELISA. Results: RORC, IL-17A and IL-22 gene expression was significantly higher in patients with psoriasis compared with healthy controls (P<0.05). In addition, a marked increase in plasma IL-17A and IL-22 levels was observed in patient group compared to controls (P<0.001). Study limitations: small number of patients. Conclusion: These data suggest that Th17 response may contribute to the pathogenesis of psoriasis.
Descritores: Psoríase/metabolismo
Queratinócitos/fisiologia
Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/fisiologia
Células Th17/metabolismo
-Psoríase/etiologia
Índice de Gravidade de Doença
RNA Mensageiro/metabolismo
Estudos de Casos e Controles
Expressão Gênica
Queratinócitos/citologia
Diferenciação Celular
Interleucinas/sangue
Interleucina-17/sangue
Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/metabolismo
Células Th17/imunologia
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME


  3 / 396 LILACS  
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Id: biblio-839349
Autor: Martins, Gabrielle R; Marinho, Rebeca C; Bezerra Junior, Rosivaldo Q; Alves, Antoniel de O; Câmara, Lilia M. C; Albuquerque-Pinto, Luiz C; Teixeira, Maria F. da S.
Título: Goat umbilical cord cells are permissive to small ruminant lentivirus infection in vitro
Fonte: Braz. j. microbiol;48(1):125-131, Jan.-Mar. 2017. graf.
Idioma: en.
Projeto: National Council of Scientific and Technological Development.
Resumo: Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture. To accomplish this goal, cells were isolated from umbilical cords; characterized phenotypically by flow cytometry analysis; differentiate into osteogenic, chondrogenic and adipogenic lineage; and submitted to viral challenge. The proliferation of goat umbilical cord cells was fast and cell monolayers formed after 15 days. These cells exhibited morphology, immunophenotype, growth characteristics, and lineage differentiation potential similar to mesenchymal stem cells of other origins. The goat umbilical cord derived cells stained positive for vimentin and CD90, but negative for cytokeratin, CD34 and CD105 markers. Syncytia and cell lysis were observed in cell monolayers infected by CAEV-Cork and MVV-K1514, showing that the cells are permissive to small ruminant lentivirus infection in vitro. These data demonstrate the proliferative competence of cells derived from goat umbilical cords and provide a sound basis for future research to standardize this cell lineage.
Descritores: Cordão Umbilical/citologia
Lentivirus/fisiologia
Células-Tronco Mesenquimais/virologia
-Osteogênese
Replicação Viral
Técnicas In Vitro
Cabras
Biomarcadores
Diferenciação Celular
Células Cultivadas
Imunofenotipagem
Técnicas de Cultura de Células
Condrogênese
Efeito Citopatogênico Viral
Adipogenia
Células-Tronco Mesenquimais/citologia
Células-Tronco Mesenquimais/metabolismo
Células-Tronco Mesenquimais/patologia
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-886180
Autor: Noronha, Samuel Marcos Ribeiro; Gragnani, Alfredo; Pereira, Thiago Antônio Calado; Correa, Silvana Aparecida Alves; Bonucci, Jessica; Ferreira, Lydia Masako.
Título: Aldefluor protocol to sort keratinocytes stem cells from skin
Fonte: Acta cir. bras;32(11):984-994, Nov. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract Purpose: To investigate the use Aldefluor® and N, N - Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor® aliquots were prepared and maintained at temperature between 2 to 8°C, or stored at -20°C. Next, the cells were collected following the standard protocol of sample preparation. Results: Best results were obtained with Aldefluor® 1.5µl and DEAB 15 µl for 1 x 106 cells, incubated at 37°C for 15 minutes. Flow cytometer range for keratinocyte stem cells separation was evaluated. There were 14.8% of stem cells separated in one sample of keratinocyte culture used to pattern the protocol. After being defined the ideal concentration, the same test pattern was performed in other keratinocyte samples. We observed a final mean of 10.8%. Conclusion: Aldefluor® has been shown as a favorable marking of epidermal keratinocyte stem cells for subsequent separation on a flow cytometer, with detection of 10.8% of epidermal keratinocyte stem cells, in this protocol.
Descritores: Células-Tronco/citologia
Queratinócitos/citologia
Diferenciação Celular/fisiologia
Citometria de Fluxo/métodos
-Pele/citologia
Biomarcadores/análise
Células Cultivadas
Protocolos Clínicos
Técnicas de Cultura de Células
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


  5 / 396 LILACS  
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Id: lil-233915
Autor: Telló, Marcos; Dias, Guilherme A. D; Haffner, Sérgio L.
Título: Modelo numérico para detecçäo de tumores / Numerical model to detect tumor
Fonte: In: Schiabel, Homero; Slaets, Annie France Frère; Costa, Luciano da Fontoura; Baffa Filho, Oswaldo; Marques, Paulo Mazzoncini de Azevedo. Anais do III Fórum Nacional de Ciência e Tecnologia em Saúde. Säo Carlos, s.n, 1996. p.654-654, ilus.
Idioma: pt.
Conferência: Apresentado em: Fórum Nacional de Ciência e Tecnologia em Saúde, 3 e Congresso Brasileiro de Engenharia Biomédica, 15 e Congresso Brasileiro de Físicos em Medicina , 6 e Congresso Brasileiro de Informática em Saúde, 5 e Encontro Brasileiro de Proteçäo Radiológica, Campos do Jordäo, 13-17 out. 1996.
Resumo: A intenção deste trabalho é a de sugerir um modelo numérico para a detecção de tumores em regiões do corpo humano. Assim, empregando a técnica dos dois eletrodos (TDE) na região em teste (RET), indica-se o Método dos Elementos Finitos (MEF), associado com um método probabilístico, para predizer o espalhamento dos potenciais elétrico medidos (calculados). Muitos eventos aleatórios afetam os potenciais e, devido a isto, o resultado do método numérico empregado (diagnóstico) caracteriza a probabilidade de detecção do tumor no ser humano.
Descritores: Eletrodos/estatística & dados numéricos
Eletrofisiologia
Neoplasias/fisiopatologia
Modelos Estatísticos
Modelos Teóricos
-Mitose
Antígenos de Diferenciação
Diferenciação Celular
Metástase Neoplásica
Método de Monte Carlo
Limites: Humanos
Responsável: BR1.1 - BIREME
BR1.1/3012.107


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Id: lil-779903
Autor: RAHMAN, Mohammad Zeshaan; SHIGEISHI, Hideo; SASAKI, Kazuki; OTA, Akira; OHTA, Kouji; TAKECHI, Masaaki.
Título: Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis
Fonte: J. appl. oral sci;24(2):153-161, Mar.-Apr. 2016. graf.
Idioma: en.
Projeto: Ministry of Education, Culture, Sports, Science and Technology.
Resumo: ABSTRACT Objective Biocompatible materials such as interconnected porous hydroxyapatite ceramics (IP-CHA) loaded with osteogenic cells and bioactive agents are part of an evolving concept for overcoming craniofacial defects by use of artificial bone tissue regeneration. Amongst the bioactive agents, melatonin (MEL) and basic fibroblast growth factor (FGF-2) have been independently reported to induce osteoblastic activity. The present in vitro study was undertaken to examine the relationship between these two bioactive agents and their combinatory effects on osteoblastic activity and mineralization in vitro. Material and Methods Mouse preosteoblast cells (MC3T3-E1) were seeded and cultured within cylindrical type of IP-CHA block (ø 4x7 mm) by vacuum-assisted method. The IP-CHA/MC3T3 composites were subjected to FGF-2 and/or MEL. The proliferation assay, alkaline phosphatase enzyme activity (ALP), mRNA expressions of late bone markers, namely Osteocalcin (OCN) and Osteopontin (OPN), and Alizarin Red staining were examined over a period of 7 days. Results FGF-2 mainly enhanced the proliferation of MC3T3-E1 cells within the IP-CHA constructs. MEL mainly induced the mRNA expression of late bone markers (OCN and OPN) and showed increased ALP activity of MC3T3 cells cultured within IP-CHA construct. Moreover, the combination of FGF-2 and MEL showed increased osteogenic activity within the IP-CHA construct in terms of cell proliferation, upregulated expressions of OCN and OPN, increased ALP activity and mineralization with Alizarin Red. The synergy of the proliferative potential of FGF-2 and the differentiation potential of MEL showed increased osteogenic activity in MC3T3-E1 cells cultured within IP-CHA constructs. Conclusion These findings indicate that the combination of FGF-2 and MEL may be utilized with biocompatible materials to attain augmented osteogenic activity and mineralization.
Descritores: Osteoblastos/efeitos dos fármacos
Fator 2 de Crescimento de Fibroblastos/farmacologia
Durapatita/farmacologia
Substitutos Ósseos/farmacologia
Melatonina/farmacologia
-Fatores de Tempo
Teste de Materiais
Calcificação Fisiológica/efeitos dos fármacos
Microscopia Eletrônica de Varredura
Diferenciação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proliferação de Células/efeitos dos fármacos
Fosfatase Alcalina/análise
Limites: Animais
Camundongos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1009000
Autor: Liu, Chunming; Yang, Bowen; Ming, Yuetong; Liu, Jianfeng; Cheng, Yunqing.
Título: Construction of an RNAi vector for knockdown of GM-ACS genes in the cotyledonary nodes of soybean
Fonte: Electron. j. biotechnol;26:40-45, Mar. 2017. ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Special Foundation for Young Scientists of Jilin Province, China.
Resumo: Background: Ethylene plays an important role in the regulation of floral organ development in soybean, and 1-aminocyclopropane-1-carboxylate synthase (ACS) is a rate-limiting enzyme for ethylene biosynthesis. However, whether ACS also regulates floral organ differentiation in soybean remains unknown. To address this, we constructed an RNAi vector to inhibit ACS expression in cotyledonary nodes. Linear DNA cassettes of RNAi-ACS obtained by PCR were used to transform soybean cotyledonary nodes. Results: In total, 131 of 139 transiently transformed plants acquired herbicide resistance and displayed GUS activities in the new buds. In comparison to untransformed seedling controls, a greater number of flower buds were differentiated at the cotyledonary node; GM-ACS1 mRNA expression levels and ethylene emission in the transformed buds were reduced. Conclusion: These results indicate that the cotyledonary node transient transformation system may be suitable for stable transformation and that the inhibition of ACS expression may be an effective strategy for promoting floral organ differentiation in soybean.
Descritores: Soja/enzimologia
Soja/genética
Interferência de RNA
Liases/metabolismo
-Soja/crescimento & desenvolvimento
Transformação Genética
Expressão Gênica
Diferenciação Celular
Reação em Cadeia da Polimerase
Regulação da Expressão Gênica de Plantas
Etilenos/biossíntese
Resistência a Herbicidas
Vetores Genéticos
Glucuronidase
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-893672
Autor: Rodini, Camila Oliveira; Lopes, Nathália Martins; Lara, Vanessa Soares; Mackenzie, Ian Campbell.
Título: Oral cancer stem cells - properties and consequences
Fonte: J. appl. oral sci;25(6):708-715, Nov.-Dec. 2017. graf.
Idioma: en.
Projeto: FAPESP (São Paulo Research Foundation).
Resumo: Abstract Research on cancer stem cells (CSCs) has greatly increased in the field of medicine and pathology; however, some conceptual misunderstandings are still present among the public as well as within the general scientific community that is not yet familiar with the subject. The very first problem is the misinterpretation of CSCs as a synonym of their normal counterparts, the well-known stem cells (SCs). Particularly in Dentistry, another common mistake is the misinterpretation of oral CSCs as normal tooth-derived SCs. The present review aims to clarify important concepts related to normal SCs and CSCs, as well as discuss the relevance of CSCs to the development, metastasis and therapy resistance of oral squamous cell carcinoma.
Descritores: Células-Tronco Neoplásicas/patologia
Neoplasias Bucais/patologia
Carcinoma de Células Escamosas/patologia
-Diferenciação Celular
Transformação Celular Neoplásica
Transição Epitelial-Mesenquimal
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-893662
Autor: Öncel Torun, Zeynep; Torun, Deniz; Baykal, Barış; Öztuna, Ali; Yeşildal, Fatih; Avcu, Ferit.
Título: Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells
Fonte: J. appl. oral sci;25(6):631-640, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Scientific and Technological Research Council of Turkey.
Resumo: Abstract Objectives: The primary purpose of this study was to examine the effects of triethylene glycol dimethacrylate (TEGDMA) on odontoclastic differentiation in the dental pulp tissue. Material and Methods: The effects of different TEGDMA dosages on the odontoclastic differentiation capability of dental pulp cells were analyzed in vitro using the following methodologies: i) flow cytometry and tartrate-resistant acid phosphatase (TRAP) staining; ii) apoptotic effects using Annexin V staining; iii) mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor (NF)-kB ligand (RANKL) genes by quantitative Real-time PCR (qRT-PCR); and iv) OPG and RANKL protein expression by enzyme-linked immunosorbent assay (ELISA). Results: TEGDMA caused relatively less odontoclastic differentiation in comparison with the control group; however, odontoclastic differentiation augmented with increasing doses of TEGDMA (p<0.05). The mRNA and protein expression of OPG was lower in TEGDMA treated pulp cells than in the control group (p<0.05). While the mRNA expression of RANKL remained unchanged compared to the control group (p>0.05), its protein expression was higher than the control group (p<0.05). In addition, TEGDMA increased the apoptosis of dental pulp cells dose dependently. Conclusions: TEGDMA reduced the odontoclastic differentiation ability of human dental pulp cells. However, odontoclastic differentiation ratios increased proportionally with the increasing dose of TEGDMA.
Descritores: Polietilenoglicóis/farmacologia
Ácidos Polimetacrílicos/farmacologia
Diferenciação Celular/efeitos dos fármacos
Polpa Dentária/efeitos dos fármacos
Fosfatase Ácida Resistente a Tartarato/efeitos dos fármacos
-Ensaio de Imunoadsorção Enzimática
Receptores de Lipopolissacarídeos/metabolismo
Polpa Dentária/citologia
Ligante RANK/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-893619
Autor: TANG, Jia; SAITO, Takashi.
Título: Human plasma fibronectin promotes proliferation and differentiation of odontoblast
Fonte: J. appl. oral sci;25(3):299-309, May-June 2017. graf.
Idioma: en.
Projeto: Japanese Society for the Promotion of Science; . Japanese Society for the Promotion of Science.
Resumo: Abstract Objective To assess the effect of fibronectin (Fn) and porcine type I collagen (PCOL) on odontoblast-like cells in vitro. Material and Methods Rat odontoblast-like cells (MDPC-23 cells) were inoculated and cultured on Fn-coated or type I collagen-coated substrates. Proliferation assay, alkaline phosphatase activity (ALP activity), mRNA expression of hard tissue-forming markers, and Alizarin red staining were investigated over a period of 10 days. Results Cells maintained a high proliferation activity on Fn and PCOL even at a low seeding concentration (0.5×104/mL) as demonstrated by CCK-8 assay. The proliferation activity of cells on Fn increases in a concentration-dependent manner while it reached a plateau after 10 µg/mL. Cells adopted long, thin and spindle shape on Fn(10-50) and PCOL. Parallel actin filaments were observed in MDPC-23 cells cultured on Fn and PCOL. ALP activity was markedly up-regulated on Fn and PCOL-coated surfaces. Importantly, gene expression of BSP (Fn10: 2.44±0.32; Fn20: 3.05±0.01; Fn30: 2.90±0.21; Fn40: 2.74±0.30; Fn50: 2.64±0.12; PCOL: 2.20±0.03) and OCN (Fn10: 2.52±0.23; Fn20: 2.28±0.24; Fn30: 2.34±0.21; Fn40: 2.34±0.25; Fn50: 2.20±0.22; PCOL: 1.56±0.16) was significantly enhanced on Fn and PCOL substrates as compared with control; moreover, expression of integrin beta 1 (ITGB1), an ubiquitous cell surface receptor was augmented in Fn(10-50) and PCOL groups simultaneously. In accordance with the ALP activity and gene expression data, calcific deposition in cells grown on Fn(10-50) and PCOL was observed as well. Conclusion Despite the limitation of this study, the findings indicate that a surface coating of Fn enhances the proliferation, differentiation and mineralization of odontoblast-like cells by activation of integrin beta 1 (ITG B1). The promoting effects of Fn on MDPC-23 cells were achieved at a comparatively lower coating concentration than type I collagen (300 µg/mL). Specifically, it is suggested that the optimum coating concentration of Fn to be 10 µg/mL.
Descritores: Diferenciação Celular/efeitos dos fármacos
Fibronectinas/farmacologia
Proliferação de Células/efeitos dos fármacos
Odontoblastos/efeitos dos fármacos
-Fatores de Tempo
Expressão Gênica
Células Cultivadas
Reprodutibilidade dos Testes
Imunofluorescência
Antraquinonas
Integrina beta1/farmacologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Colágeno Tipo I/farmacologia
Fosfatase Alcalina/análise
Limites: Humanos
Animais
Ratos
Responsável: BR1.1 - BIREME



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