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Id: biblio-1289389
Autor: Morales, Alberto Piamo; García Rojas, Mayra A.
Título: Efecto de la diabetes sobre la espermatogénesis / Effect of diabetes on spermatogenesis
Fonte: Rev. cuba. endocrinol;32(1):e154, 2021.
Idioma: es.
Resumo: La evidencia clínica que ha permitido relacionar la diabetes mellitus con la infertilidad se basa en la importancia del metabolismo de la glucosa durante el proceso de espermatogénesis, debido a que en los episodios tanto de hipoglucemia como de hiperglucemia pueden ocurrir cambios epigenéticos en algunas proteínas involucradas en la espermatogénesis. En la presente comunicación se describen los aspectos teóricos de los efectos de la diabetes sobre el líquido seminal con énfasis en la espermatogénesis(AU)

The clinical evidence that has made it possible to link diabetes mellitus with infertility is based on the importance of glucose metabolism during the spermatogenesis process, because in episodes of both hypoglycemia and hyperglycemia, epigenetic changes can occur in some proteins involved in spermatogenesis. This communication describes the theoretical aspects of the effects of diabetes on seminal fluid with emphasis on spermatogenesis(AU)
Descritores: Espermatogênese
Diabetes Mellitus/epidemiologia
Hiperglicemia/etiologia
Hipoglicemia/etiologia
Infertilidade/terapia
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-1279528
Autor: Abud, Luciane Lily; Schimming, Bruno Cesar.
Título: Seasonal variations of male reproductive parameters of Tomodon dorsatus from Southeastern Brazil / Variações sazonais dos parâmetros reprodutivos do macho de Tomodon dorsatus do sudeste brasileiro
Fonte: Pesqui. vet. bras = Braz. j. vet. res;41:e06725, 2021. graf.
Idioma: en.
Resumo: The morphology of the male reproductive tract of Tomodon dorsatus was described in the austral seasons of the year considering macroscopic and microscopic variables. For this purpose, 56 specimens from the herpetological collection of the "Instituto Butantan" were used. Fragments of the testes, kidneys and ductus deferens were collected and submitted to histological routine. The peak of the testicular volume was observed in the summer and the epithelium of the seminiferous tubules had higher height in the summer (p=0.001). The testes were active throughout the year, however, the spermiogenesis peaked in the summer. There were spermatozoa in the lumen of the ductus deferens in all seasons of the year. Renal length was higher in autumn (p=0.027), and renal width did not show a significant increase (p=0.237). The diameter and epithelial height of the sexual segment of the kidney (SSK) showed hypertrophy in winter and spring, coinciding with the mating period. Based on findings of this study, we can suggest that, at the population level, the reproductive cycle of T. dorsatus can be considered seasonal semi-synchronous, due to the peak of spermiogenic activity in the hot season, and discontinuous at the individual level.(AU)

A morfologia do trato reprodutivo do macho de Tomodon dorsatus foi descrita nas estações climáticas do ano com base em variáveis macroscópicas e microscópicas. Para isto, foram usados 56 espécimes oriundos da coleção herpetológica do Instituto Butantan. Fragmentos dos testículos, rins e ductos deferentes foram coletados e submetidos à rotina histológica. O volume testicular foi maior no verão e o epitélio dos túbulos seminíferos mostrou uma maior altura no verão (p=0.001). Os testículos estavam ativos durante todo o ano, contudo, a espermiogênese foi maior no verão. Espermatozoides foram encontrados no lúmen do ducto deferente em todas as estações do ano. O comprimento renal foi maior no outono (p=0.027), e a largura renal não mostrou um aumento significativo (p=0.237). O diâmetro e a altura epitelial do segmento sexual do rim (SSR) mostrou hipertrofia nas estações inverno e primavera, coincidindo com o período reprodutivo. Com base nestes resultados, pode-se sugerir que, em nível populacional, o ciclo reprodutivo de T. dorsatus possa ser considerado semi-sincrônico sazonal, devido à atividade espermiogênica na estação quente, e descontínuo em nível individual.(AU)
Descritores: Estações do Ano
Testículo
Ducto Deferente
Hipertrofia
-Espermatogênese
Bothrops
Parâmetros de Referência
Limites: Animais
Masculino
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-676151
Autor: Chehreie, Shima; Rabzia, Arezou; Farhadi-mesterkhani, Mohammad.
Título: Maternal water deprivation affects the spermatogenesis of the offspring rats / La privación del agua en la etapa materna de ratas afecta la espermatogénesis de sus crías
Fonte: Int. j. morphol;31(1):156-161, mar. 2013. ilus.
Idioma: es.
Projeto: Islamic Azad University and Kermanshah University of Medical Sciences.
Resumo: Prenatal stresses such as water deprivation affect developmental process of embryo. This study evaluated the effects of water deprivation in pregnant mother on histological parameters of testis of offspring. Pregnant rats were divided into two groups (control and experimental). In experimental animals, water was removed from the ewes for 48h at the end of third trimester of gestation (19-21th days). Histopathology and histomorphic analysis and also TUNEL assay on offspring's testes were performed at pubertal age (60 days). The sperm motility either compared between two groups. The results showed that prenatal water deprivation induced histopathological alteration such as epithelium vacuolization, sloughing and detachments (P<0.01). Morphometrical data showed that prenatal water deprivation decreased tubular diameter and reduce epithelium height (P<0.01). Johnsen's score showed poor spermatogenesis in experimental group (P=0.001). The percent of germ cell apoptosis was increased in offspring's testes of rats born to mothers exposed to stress during pregnancy (P=0.000). The increased number of Multinucleated cells in the seminiferous lumen (P=0.000) in parallel with decreased number of sertoli cells (P=0.03) showed adverse effect of prenatal water deprivation on blood testis barrier. Present study revealed that prenatal water deprivation had injurious effect on developmental process of testes that affects on both germ cells and sertoli cells and had noxious effect on sperm parameters. These data confirm that prenatal stress disrupts normal spermatogenesis of offspring.

El estrés prenatal, como la privación del agua, afecta el proceso de desarrollo embrionario. Este estudio evaluó los efectos de la falta de agua en la rata preñada sobre los parámetros histológicos del testículo de las crías. Las ratas preñadas fueron divididas en dos grupos (control y experimental). En los animales de experimentación, se eliminó el agua durante 48 h al final del término de la gestación (19-21 días). Junto al análisis histopatológico e histomorfométrico se realizó el ensayo TUNEL en los testículos de las crías en la edad puberal (60 días). La motilidad de los espermatozoides se comparó entre los dos grupos. Los resultados mostraron que la privación de agua prenatal induce alteraciones histopatológicas tales como vacuolización del epitelio, descamación y desunión (P<0,01). Los datos morfométricos mostraron que con la privación de agua prenatal hubo disminución del diámetro tubular y se redujo la altura del epitelio (P<0,01). El score de Johnsen mostró una espermatogénesis deficiente en el grupo experimental (p = 0,001). El porcentaje de apoptosis de las células germinales se incrementó en los testículos de las crías de las ratas nacidas de madres expuestas a estrés durante el embarazo (p = 0,000). Un aumento del número de células multinucleadas en el lumen seminífero (P = 0,000) junto a la disminución del número de células de Sustento (P = 0,03) demostró el efecto adverso de la privación de agua prenatal en la barrera hemato-testicular. El presente estudio reveló que la falta de agua prenatal tuvo un efecto perjudicial en el proceso de desarrollo de los testículos, lo que afecta a las células germinales y los sustentocitos, y tuvo un efecto nocivo sobre los parámetros seminales. Estos datos confirman que el estrés prenatal altera la espermatogénesis normal de la descendencia.
Descritores: Testículo/patologia
Privação de Água
-Efeitos Tardios da Exposição Pré-Natal
Espermatogênese
Ratos Sprague-Dawley
Marcação In Situ das Extremidades Cortadas
Microscopia
Limites: Animais
Masculino
Feminino
Gravidez
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1278437
Autor: Rodrigues, Cândido Carvalho; Caixeta, Maxwell Batista; Rocha, Thiago Lopes.
Título: New Insights into the Gametogenesis of Biomphalaria glabrata (Mollusca, Gastropoda, Pulmonata): Implications for Histopathological Assessment
Fonte: Braz. arch. biol. technol;64:e21200574, 2021. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de Goiás; . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Abstract The reproductive system has a fundamental role in population dynamics and several reproduction strategies have been shaped by the environment over time. Many environmental pressures are generated by releasing pollutants, as endocrine disruptors, that can affect the reproductive system of individuals, among them invertebrates. The freshwater snails Biomphalaria spp. are used as biomonitor in several ecotoxicological studies; however, there are few studies about gametogenesis and morphology of reproductive snail cells, which could be used as a new biomarker. In this sense, the current study aims to characterize Biomphalaria glabrata gametogenesis, bringing new histomorphometric parameters for germinative cells. Results showed that the hermaphrodite tissue is formed by several acini with simple pavement epithelium with germinative and somatic cells. Oogenesis was classified into five developmental stages (OI to OV) according to diameter, nucleus area, total area, and follicular cell development, and then classified into previtellogenic and vitellogenic oocytes. The spermatogenesis was classified into spermatogonia (Spg), spermatocytes (Spc) and spermatids that were subdivided into five stages (Spt I to Spt V) according to cytoplasm losing, and nucleus spiralization along with Sertoli cells development. Thus, the present study highlights the gametogenesis of B. glabrata with new histomorphometric parameters, which can be an important tool for ecotoxicological and molluscicidal developmental further studies.
Descritores: Oogênese
Caramujos
Espermatogênese
Organismos Hermafroditas
-Técnicas, Medidas, Equipamentos de Medição
Ecotoxicologia/métodos
Responsável: BR1.1 - BIREME


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Medeiros, Maria Angelina da Silva
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Id: biblio-892958
Autor: Barroso, Leocácio Venícius Sousa; Reges, Ricardo; Cerqueira, João Batista Gadelha; Miranda, Eduardo P; Alcantara, Rafael Jorge Alves de; Jamacaru, Francisco Vagnaldo F; Moraes, Manoel Odorico de; Medeiros, Maria Angelina da Silva; Gonzaga-Silva, Lúcio Flávio.
Título: Impact of testicular sperm extraction and testicular sperm aspiration on gonadal function in an experimental rat model
Fonte: Int. braz. j. urol;44(1):172-179, Jan.-Feb. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Purpose To assess the impact of sperm retrieval on the gonadal function of rats with impaired spermatogenesis by comparing testicular sperm extraction (TESE) to aspiration (TESA). The efficacy of these procedures to sperm obtainment was also compared. Materials and Methods A pilot study showed impaired spermatogenesis, but normal testosterone (T) production after a bilateral orchidopexy applied to 26 rats, which were randomly assigned into four groups: TESE (n=7), TESA (n=7), SHAM (n=6) and Control (n=6). The T levels were measured through comparative analysis after the orchidopexy. Results There was no statistical difference in the animal's baseline T levels after orchidopexy in comparison to the controls: the TESE and TESA groups, 6.66±4.67ng/mL; the SHAM group (orchidopexy only), 4.99±1.96ng/mL; and the Control, 4.75±1.45ng/mL, p=0.27. Accordingly, no difference was found in the postoperative T levels: TESE, 5.35±4.65ng/mL; TESA, 3.96±0.80ng/mL; SHAM, 3.70±1.27ng/mL; p=0.4. The number of sperm cells found through TESE (41.0±7.0) was significantly larger than that found through TESA (21.3±8.1, p=0.001). Moreover, higher tissue weight was found through TESE (0.09±0.02g versus 0.04±0.04g, p=0.04). Conclusions The testicular sperm capture performed in rats through extraction or aspiration, after orchidopexy, did not significantly decrease the T levels. The amount of sperm found through testicular sperm extraction was higher than that through testicular sperm aspiration.
Descritores: Motilidade Espermática/fisiologia
Espermatogênese/fisiologia
Espermatozoides/fisiologia
Testículo/fisiologia
Recuperação Espermática/efeitos adversos
-Testículo/cirurgia
Testosterona/biossíntese
Distribuição Aleatória
Projetos Piloto
Ratos Wistar
Modelos Animais
Orquidopexia/métodos
Limites: Animais
Masculino
Ratos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Boaventura, Gilson Teles
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Id: biblio-1019894
Autor: Corrêa, Lanna Beatriz Neves Silva; Costa, Carlos Alberto Soares da; Ribas, José Antônio Silva; Boaventura, Gilson Teles; Chagas, Mauricio Alves.
Título: Antioxidant action of alpha lipoic acid on the testis and epididymis of diabetic rats: morphological, sperm and immunohistochemical evaluation
Fonte: Int. braz. j. urol;45(4):815-824, July-Aug. 2019. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: ABSTRACT Introduction Chronic hyperglycemia is caused by diabetes mellitus-committed genital morphophysiology, and oxidative stress is one of the main factors involved in this process. Alpha lipoic acid (ALA) can prevent metabolic and morphological changes in diabetic individuals. Objectives In present study, we evaluated the effects of regular ALA consumption on the spermatogenesis and histoarchitecture in the male genital system of diabetic rats. Materials and Methods Thirty-two Wistar rats were divided into groups: Control (CG); Diabetic Control (DCG), receiving commercial diet: ALA Group (ALAG) and Diabetic ALA Group (DALAG), fed diets with added ALA (300 mg/Kg bw). The diabetic groups received a single injection of streptozotocin (60 mg/kg). After sixty days of the diet, the animals were euthanized, and semen, testis and epididymis samples were collected. A histomorphometric analysis was performed to determine the epithelial height, tubular and luminal diameter, tubular and luminal area of seminiferous tubules and each epididymal region. Sertoli cells were evidenced using the antivimenti antibody and were quantified. The results were statistically analyzed by the ANOVA test. Results At the end of the experiment, the DALAG glycemia was significantly lower than DCG. The histomorphometric parameters of the seminiferous and epididymal tubules did not show improvement in the DALAG. However, there was an improvement in the DALAG in terms of the concentration, motility and percentage of spermatic pathologies, as well as in the number of Sertoli cells (p<0.001). Conclusions The results demonstrated that supplementation with the ALA antioxidant retards testicular lesions and preserve the process of spermatogenesis in diabetes.
Descritores: Espermatozoides/efeitos dos fármacos
Testículo/efeitos dos fármacos
Ácido Tióctico/farmacologia
Diabetes Mellitus Experimental/patologia
Epididimo/efeitos dos fármacos
Antioxidantes/farmacologia
-Células de Sertoli
Contagem de Espermatozoides
Motilidade Espermática/efeitos dos fármacos
Espermatogênese/efeitos dos fármacos
Espermatogênese/fisiologia
Espermatozoides/fisiologia
Testículo/fisiopatologia
Testículo/patologia
Imuno-Histoquímica
Distribuição Aleatória
Reprodutibilidade dos Testes
Ratos Wistar
Diabetes Mellitus Experimental/fisiopatologia
Epididimo/patologia
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-950727
Autor: Urriola-Muñoz, Paulina; Lizama, Carlos; Lagos-Cabré, Raúl; Reyes, Juan G; Moreno, Ricardo D.
Título: Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
Fonte: Biol. Res;47:1-12, 2014. ilus, graf.
Idioma: en.
Projeto: FONDECYT.
Resumo: BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. RESULTS: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of spermatogenesis. Interestingly their protein levels and cell surface localization in adult rats were stage-specific, suggesting activation of these enzymes at particular events of rat spermatogenesis. CONCLUSIONS: Therefore, these results show that ADAM10 and ADAM17 protein levels and subcellular (cell surface) localization are regulated during rat spermatogenesis.
Descritores: Espermatogênese/fisiologia
Espermatozoides/metabolismo
Proteínas ADAM/metabolismo
-Túbulos Seminíferos/química
Células de Sertoli/citologia
Células de Sertoli/metabolismo
Espermátides/citologia
Espermátides/metabolismo
Testículo/anatomia & histologia
RNA Mensageiro/análise
Imuno-Histoquímica
Diferenciação Celular/fisiologia
Ratos Sprague-Dawley
Apoptose/fisiologia
Receptor fas/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proteínas ADAM/análise
Proteína ADAM10
Proteína ADAM17
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Machado, Márcia Rita Fernandes
Miglino, Maria Angélica
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Id: biblio-1134471
Autor: Simões, Luciana Senna; Sasahara, Taís Harumi de Castro; Favaron, Phelipe Oliveira; Leal, Leonardo Martins; Oliveira, Fabrício Singaretti de; Fratini, Paula; Berger, Trish; Machado, Marcia Rita Fernandes; Miglino, Maria Angélica.
Título: The morphology of paca (Cuniculus paca) testis with high dose of letrozole an aromatase inhibitor / La morfología del testículo de paca (Cuniculus paca) con altas dosis de letrozol, un inhibidor de la aromatasa
Fonte: Int. j. morphol;38(6):1525-1527, Dec. 2020. graf.
Idioma: en.
Resumo: SUMMARY: The study reported the influence of the high and acute dose of Letrozole on the testis morphology in paca (Cuniculus paca), an aromatase inhibitor that reduces the endogenous estrogen, the essential hormone for spermatogenesis. Morphological changes were observed in seminiferous epithelium with germ cells with apoptotic characteristics and presence of vacuoles and nuclei in pycnose.

RESUMEN: El objetivo de este estudio fue analizar la influencia de una dosis alta de Letrozol en la morfología de los testículos de la paca (Cuniculus paca), un inhibidor de la aromatasa que reduce el estrógeno endógeno, la hormona esencial para la espermatogénesis. Se observaron cambios morfológicos en el epitelio seminífero con células germinales con características apoptóticas y la presencia de vacuolas y núcleos en picnosis.
Descritores: Testículo/efeitos dos fármacos
Inibidores da Aromatase/administração & dosagem
Cuniculidae
Letrozol/administração & dosagem
-Epitélio Seminífero/efeitos dos fármacos
Espermatogênese/efeitos dos fármacos
Imuno-Histoquímica
Orquiectomia
Microscopia Eletrônica de Transmissão
Células Germinativas/efeitos dos fármacos
Limites: Animais
Masculino
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: biblio-950898
Autor: Diao, Ruiying; Cai, Xueyong; Liu, Lu; Yang, Lihua; Duan, YongGang; Cai, Zhiming; Gui, Yaoting; Mou, Lisha.
Título: In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
Fonte: Biol. Res;51:12, 2018. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Guangdong Natural Science Foundation; . Shenzhen Foundation of Science and Technology; . Medical Scientific Research Foundation of Guangdong.
Resumo: BACKGROUND: Chemokine (C-C motif) receptor 6 (CCR6) is present in sperm and plays a significant role in sperm motility and chemotaxis acting in the reproductive tracts. However, the expression and functional significance of CCR6 in testis are still poorly understood, especially in the process of spermatogenesis. METHODS AND RESULTS: CCR6 was expressed in spermatogenic cell lines and its expression was shown in an age-dependent upregulation manner from puberty to adulthood in mouse testis. Immunostaining results confirmed the localization of CCR 6 in testis. Further chemotaxis assays demonstrated that spermatogenic cells GC-1 and -2 exhibited a directional movement toward CCR6-specific ligand such as CCL20 or Sertoli cells in vitro. CONCLUSIONS: The present findings indicate that CCR6 is involved in the chemotaxis of spermatogenic cells in vitro and promotes chemotaxis under non-inflammatory conditions during normal spermatogenesis.
Descritores: Espermatogênese/fisiologia
Quimiotaxia/fisiologia
Criptorquidismo/metabolismo
Quimiocina CCL20/metabolismo
Receptores CCR6/metabolismo
-Células de Sertoli
Motilidade Espermática/fisiologia
Testículo/fisiologia
Imuno-Histoquímica
Western Blotting
Imunofluorescência
Camundongos Endogâmicos C57BL
Limites: Humanos
Animais
Masculino
Camundongos
Coelhos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1011418
Autor: Mohaqiq, Mahdi; Movahedin, Mansoureh; Mazaheri, Zohreh; Amirjannati, Naser.
Título: In vitro transplantation of spermatogonial stem cells isolated from human frozen-thawed testis tissue can induce spermatogenesis under 3-dimensional tissue culture conditions
Fonte: Biol. Res;52:16, 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Sperm production is one of the most complex biological processes in the body. In vitro production of sperm is one of the most important goals of researches in the field of male infertility treatment, which is very important in male cancer patients treated with gonadotoxic methods and drugs. In this study, we examine the progression of spermatogenesis after transplantation of spermatogonial stem cells under conditions of testicular tissue culture. RESULTS: Testicular tissue samples from azoospermic patients were obtained and then these were freeze-thawed. Spermatogonial stem cells were isolated by two enzymatic digestion steps and the identification of these cells was confirmed by detecting the PLZF protein. These cells, after being labeled with DiI, were transplanted in azoospermia adult mice model. The host testes were placed on agarose gel as tissue culture system. After 8 weeks, histomorphometric, immunohistochemical and molecular studies were performed. The results of histomorphometric studies showed that the mean number of spermatogonial cells, spermatocytes and spermatids in the experimental group was significantly more than the control group (without transplantation) (P < 0.05) and most of the cells responded positively to the detection of DiI. Immunohistochemical studies in host testes fragments in the experimental group express the PLZF, SCP3 and ACRBP proteins in spermatogonial cells, spermatocyte and spermatozoa, respectively, which confirmed the human nature of these cells. Also, in molecular studies of PLZF, Tekt1 and TP1, the results indicated that the genes were positive in the test group, while not in the control group. CONCLUSION: These results suggest that the slow freezing of SSCs can support the induction of spermatogenesis to produce haploid cells under the 3-dimensional testicular tissue culture.
Descritores: Espermatogênese/fisiologia
Espermatogônias/transplante
Testículo/citologia
Criopreservação/métodos
Transplante de Células-Tronco/métodos
Limites: Humanos
Animais
Masculino
Camundongos
Responsável: CL1.1 - Biblioteca Central



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