Base de dados : LILACS
Pesquisa : G05.308.385 [Categoria DeCS]
Referências encontradas : 21 [refinar]
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  1 / 21 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-839310
Autor: Zhang, J; Ma, J; Wang, H; Guo, L; Li, J.
Título: Serum microRNA-30c levels are correlated with disease progression in Xinjiang Uygur patients with chronic hepatitis B
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(6):e6050, 2017. tab, graf.
Idioma: en.
Resumo: We aimed to investigate the potential role and mechanism of microRNA-30c (miR-30c) in the pathological development of chronic hepatitis B (CHB). The serum levels of miR-30c in hepatitis B virus (HBV) carrier Xinjiang Uygur patients with inactive, low-replicative, high-replicative and HBe antigen-positive CHB were investigated. HepG2 cells were co-transfected with pHBV1.3 and miR-30c mimic or inhibitor or scramble RNA. The effects of miR-30c dysregulation on HBV replication and gene expression, cell proliferation and cell cycle were then investigated. miR-30c was down-regulated in Xinjiang Uygur patients with CHB compared to healthy controls and its expression level discriminated HBV carrier patients with inactive, low-replicative, high-replicative and HBe antigen-positive risk for disease progression. Overexpression of miR-30c significantly inhibited HBV replication and the expressions of HBV pgRNA, capsid-associated virus DNA and Hbx in hepatoma cells. Moreover, overexpression of miR-30c significantly inhibited cell proliferation and delayed G1/S phase transition in hepatoma cells. Opposite effects were obtained after suppression of miR-30c. Our results indicate that miR-30c was down-regulated in Xinjiang Uygur patients with CHB, and miR-30c levels could serve as a marker for risk stratification of HBV infection. Down-regulation of miR-30c may result in the progression of CHB via promoting HBV replication and cell proliferation.
Descritores: Progressão da Doença
Vírus da Hepatite B/genética
Hepatite B Crônica/sangue
MicroRNAs/sangue
-Carcinoma Hepatocelular/genética
Carcinoma Hepatocelular/patologia
Proliferação Celular/genética
China
Regulação para Baixo
Regulação Viral da Expressão Gênica
Hepatite B Crônica/etnologia
Neoplasias Hepáticas/genética
Neoplasias Hepáticas/patologia
Aprendizagem em Labirinto
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Responsável: BR1.1 - BIREME


  2 / 21 LILACS  
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Texto completo SciELO Brasil
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Id: lil-788999
Autor: Silva Junior, Haroldo Cid da; Pestana, Cristiane Pinheiro; Galler, Ricardo; Medeiros, Marco Alberto.
Título: Solubility as a limiting factor for expression of hepatitis A virus proteins in insect cell-baculovirus system
Fonte: Mem. Inst. Oswaldo Cruz;111(8):535-538, Aug. 2016. graf.
Idioma: en.
Resumo: The use of recombinant proteins may represent an alternative model to inactivated vaccines against hepatitis A virus (HAV). The present study aimed to express the VP1 protein of HAV in baculovirus expression vector system (BEVS). The VP1 was expressed intracellularly with molecular mass of 35 kDa. The VP1 was detected both in the soluble fraction and in the insoluble fraction of the lysate. The extracellular expression of VP1 was also attempted, but the protein remained inside the cell. To verify if hydrophobic characteristics would also be present in the HAV structural polyprotein, the expression of P1-2A protein was evaluated. The P1-2A polyprotein remained insoluble in the cellular extract, even in the early infection stages. These results suggest that HAV structural proteins are prone to form insoluble aggregates. The low solubility represents a drawback for production of large amounts of HAV proteins in BEVS.
Descritores: Baculoviridae/química
Baculoviridae/metabolismo
Vírus da Hepatite A/química
Proteínas Virais/biossíntese
-Baculoviridae/genética
Regulação Viral da Expressão Gênica
Vetores Genéticos
Processamento de Proteína Pós-Traducional
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Solubilidade
Proteínas Virais/química
Proteínas Virais/genética
Responsável: BR1.1 - BIREME


  3 / 21 LILACS  
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Id: lil-759282
Autor: Zheng, Qi; Xu, Jun; Gao, Huihui; Tao, Ran; Li, Wei; Shang, Shiqiang; Gu, Weizhong.
Título: Receptor expression and responsiveness of human peripheral blood mononuclear cells to a human cytomegalovirus encoded CC chemokine
Fonte: Braz. j. infect. dis;19(4):403-409, July-Aug. 2015. tab, ilus.
Idioma: en.
Projeto: National Science and Technology Support Program of China; . Natural Science Foundation of Zhejiang Province.
Resumo: Human cytomegalovirus is a ubiquitous pathogen that infects the majority of the world's population. After long period of time co-evolving with human being, this pathogen has developed several strategies to evade host immune surveillance. One of the major trick is encoding homologous to those of the host organism or stealing host cellular genes that have significant functions in immune system. To date, we have found several viral immune analogous which include G protein coupled receptor, class I major histocompatibility complex and chemokine. Chemokine is a small group of molecules which is defined by the presence of four cysteines in highly conserved region. The four kinds of chemokines (C, CC, CXC, and CX3C) are classified based on the arrangement of 1 or 2 N-terminal cysteine residues. UL128 protein is one of the analogous that encoded by human cytomegalovirus that has similar amino acid sequences to the human CC chemokine. It has been proved to be one of the essential particles that involved in human cytomegalovirus entry into epithelial/endothelial cells as well as macrophages. It is also the target of potent neutralizing antibodies in human cytomegalovirus-seropositive individuals. We had demonstrated the chemotactic trait of UL128 protein in our previous study. Recombinant UL128 in vitrohas the ability to attract monocytes to the infection region and enhances peripheral blood mononuclear cell proliferation by activating the MAPK/ERK signaling pathway. However, the way that this viral encoded chemokine interacting with peripheral blood mononuclear cells and the detailed mechanism that involving the virus entry into host cells keeps unknown. Here we performed in vitroinvestigation into the effects of UL128 protein on peripheral blood mononuclear cell's activation and receptor binding, which may help us further understand the immunomodulatory function of UL128 protein as well as human cytomegalovirus diffusion mechanism.
Descritores: Quimiocinas CC
Citomegalovirus
Regulação Viral da Expressão Gênica/genética
Leucócitos Mononucleares/virologia
Glicoproteínas de Membrana/imunologia
Proteínas do Envelope Viral/imunologia
-Células Cultivadas
Reagentes para Ligações Cruzadas
Quimiocinas CC/genética
Quimiocinas CC/imunologia
Citomegalovirus/genética
Citomegalovirus/imunologia
Receptores de Quimiocinas/genética
Proteínas Recombinantes/imunologia
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 21 LILACS  
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Texto completo SciELO Saúde Pública
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Id: lil-744830
Autor: Gomes, Vanessa Santana; Amador, Tânia Alves.
Título: Studies published in indexed journals on lawsuits for medicines in Brazil: a systematic review / Estudios publicados en revistas indexadas acerca de decisiones judiciales para el acceso a los medicamentos en Brasil: una revisión sistemática / Estudos publicados em periódicos indexados sobre decisões judiciais para acesso a medicamentos no Brasil: uma revisão sistemática
Fonte: Cad. saúde pública = Rep. public health;31(3):451-462, 03/2015. tab, graf.
Idioma: en.
Resumo: The aim of this systematic review was to identify and characterize articles in indexed scientific journals with quantitative data surveys on administrative or legal proceedings for access to medicines. The SciELO, LILACS, MEDLINE via PubMed, Embase, and Scopus databases were used. We identified 45 articles, of which 17 were selected. The larger studies, each covering between 2,000 and 2,927 lawsuits, were done in the states of São Paulo, Rio de Janeiro, and Santa Catarina, Brazil. Eleven studies specified the type of legal representation, of which six examined cases with public attorneys and five with private attorneys. Only two studies reported whether the lawsuit was individual or class action, and in both the claims were individual. Since the majority of the medicines requested in the lawsuits were medium to high-cost, the review indicates that lawsuits contributed to the incorporation of these drugs into current pharmaceutical care in Brazil.

El objetivo de esta revisión sistemática fue identificar y caracterizar los artículos disponibles en revistas científicas indexadas en bases de datos electrónicas, que llevaron a cabo un estudio cuantitativo de datos, procedimientos administrativos o judiciales sobre la cuestión del acceso a los medicamentos a través de demandas judiciales. Los estudios fueron localizados en las bases de datos SciELO, LILACS, MEDLINE vía PubMed, Embase, Scopus. Se identificaron 45 artículos, de los cuales se seleccionaron 17. Los estudios que se llevaron a cabo engloban de 2.000 a 2.927 procesos judiciales en São Paulo, Río de Janeiro y Santa Catarina, Brasil. En once estudios se realizaron encuestas a los representantes legales de la acción judicial. En seis estudios predominó la representación pública legal y en cinco abogados privados. Sólo dos estudios examinaron si la acción era individual o colectiva y en los dos hubo prevalencia de acciones individuales. Como la mayoría de los medicamentos estaba involucrada en acciones legales de medio y alto coste, se cree que las demandas han contribuido a la incorporación de fármacos en la política pública actual.

O objetivo desta revisão sistemática foi identificar e caracterizar artigos disponíveis em periódicos científicos indexados em bases eletrônicas, que realizaram levantamento de dados quantitativo, em processos administrativos ou judiciais, sobre a questão do acesso a medicamentos por meio de ações judiciais. Foram usadas as bases de dados SciELO, LILACS, MEDLINE via PubMed, Embase e Scopus. Identificamos 45 artigos, dos quais foram selecionados 17 artigos. Os estudos com faixa de 2.000 a 2.927 processos foram conduzidos em São Paulo, Rio de Janeiro e Santa Catarina, Brasil. Em 11 estudos foram pesquisadas qual a representação jurídica da ação. Em seis estudos predominaram a representação de advogados públicos e em cinco particulares. Somente dois estudos observaram se a ação era coletiva ou individual, sendo que nas duas pesquisas a prevalência era de ações individuais. Como a maioria dos medicamentos envolvidos nas ações é de médio e alto custo, acredita-se que as demandas judiciais tenham contribuído para incorporação de medicamentos nas ações de assistência farmacêutica atuais.
Descritores: Bacteriófago lambda/genética
DNA Viral/fisiologia
Genes de Troca
Instabilidade Genômica
-Sítios de Ligação
DNA Viral/química
Regulação Viral da Expressão Gênica
Lisogenia/genética
Modelos Genéticos
Mutação
Conformação de Ácido Nucleico
Regiões Operadoras Genéticas
Processos Estocásticos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 21 LILACS  
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Texto completo SciELO Venezuela
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Id: lil-740328
Autor: Torres Espíndola, Luz María; Arellano Galindo, José; Velazquez Cruz, Rafael; Castillejos López, Manuel de Jesús.
Título: Factores que participan en la interacción huésped-agente patógeno en el riesgo de Linfoma de Hodgkin inducido por el virus Epstein Barr / Factors involved in host-pathogen interaction for the risk of Hodgkin lymphoma induced by Epstein Barr virus
Fonte: Invest. clín;54(3):311-324, sep. 2013. tab.
Idioma: es.
Resumo: El linfoma de Hodgkin (LH) es una neoplasia del sistema linfático. La incidencia mundial anual del LH es de 3-10/100,000 habitantes. El mecanismo mediante el cual se lleva a cabo la transformación celular no es completamente claro; sin embargo, algunas evidencias parecen indicar que ciertos virus oncogénicos como el virus Epstein Barr (VEB), pueden tener alto impacto en la patogénesis de la linfoproliferación. También algunos factores genéticos y ambientales pueden estar involucrados, pues se ha encontrado una alta incidencia de casos de LH entre individuos de una misma familia que comparten características genéticas y conviven en un mismo ambiente. En México se han realizado estudios encaminados a conocer la prevalencia del VEB en pacientes con LH y se ha encontrado la presencia de este virus hasta en el 64,2%. El VEB ha sido detectado en las Células Reed Sternberg (CRS) y en Células de Hodgkin (CH) en el 50% de los casos de LH clásico. No se ha dado hasta ahora una explicación satisfactoria, pero se ha propuesto que la variabilidad geográfica y la variabilidad inmunológica desempeñan un papel determinante en la positividad del VEB en LH. A pesar de los avances que hasta ahora se tienen, no existen suficientes evidencias que permitan establecer una clara asociación entre los factores del huésped, el medio ambiente y el agente patógeno en el riesgo de la linfoproliferación que conduce al desarrollo de LH. La presente revisión tiene como objetivo analizar algunos de los factores de riesgo que influyen durante la interacción huésped, agente patógeno y medio ambiente en la etiología del LH.

Hodgkin lymphoma (HL) is a neoplasm characterized by malignant cells called Reed Sternberg and Hodgkin's cells in the lymphatic system. Such cells comprise 1% of the tumor while the remainder is made up of lymphocytes, histiocytes, eosinophils and plasma non-neoplastic cells. The annual global incidence of HL is 3-10/100,000 inhabitants and is most commonly found in young adults. The mechanism by which cell transformation is accomplished is not entirely clear; however, some evidences suggest that oncogenic viruses like the Epstein Barr virus (EBV) may have a high impact on the pathogenesis of lymphoproliferation. Genetic and environmental factors could be involved, since it has been found a high incidence of HL among members of the same family. In Mexico, there have been studies to determine the prevalence of EBV in patients with HL and found the presence of this virus in up to 64.2% of the cases. EBV has been detected in the Reed Sternberg cells and Hodgkin cells in 50% of cases of classical HL. There is not a satisfactory explanation for this, but it has been proposed that geographic and immunological variabilities play a role in the positivity of EBV in HL. However, despite recent advances in the field, there is insufficient evidence to show a clear association between host factors, environment and pathogens, and the risk of lymphoproliferation leading to the development of HL. This review aims to give an overview about the risk factors that influence the interaction of host, pathogens and environment in the etiology of HL.
Descritores: Infecções por Vírus Epstein-Barr/virologia
Interações Hospedeiro-Patógeno
/fisiologia
HERPESVIRUS ABBREVIATIONS AS TOPIC, HUMAN/fisiologia
Doença de Hodgkin/virologia
-Biomarcadores Tumorais
Transformação Celular Viral
DNA Viral/genética
Infecções por Vírus Epstein-Barr/imunologia
Regulação Viral da Expressão Gênica
/genética
HERPESVIRUS ABBREVIATIONS AS TOPIC, HUMAN/genética
/imunologia
HERPESVIRUS ABBREVIATIONS AS TOPIC, HUMAN/imunologia
Doença de Hodgkin/diagnóstico
Doença de Hodgkin/epidemiologia
Evasão da Resposta Imune
Hospedeiro Imunocomprometido
Risco
Fatores de Risco
Células de Reed-Sternberg/virologia
Latência Viral
Proteínas Virais/fisiologia
Limites: Feminino
Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


  6 / 21 LILACS  
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Texto completo SciELO Brasil
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Id: lil-735849
Autor: Tavares, J.G.P.; Vasques, E.R.; Arida, R.M.; Cavalheiro, E.A.; Cabral, F.R.; Torres, L.B.; Menezes-Rodrigues, F.S.; Jurkiewicz, A.; Caricati-Neto, A.; Godoy, C.M.G.; Gomes da Silva, S..
Título: Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(2):140-145, 02/2015. tab, graf.
Idioma: en.
Projeto: CNPq, FAPESP.
Resumo: The present study evaluated electrocardiographic alterations in rats with epilepsy submitted to an acute myocardial infarction (AMI) model induced by cardiac ischemia and reperfusion. Rats were randomly divided into two groups: control (n=12) and epilepsy (n=14). It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure. In addition, significant alterations were observed in electrocardiogram intervals during the stabilization, ischemia, and reperfusion periods of rats with epilepsy compared to control rats. It was noted that rats with epilepsy presented a significant increase in the QRS interval during the stabilization period in relation to control rats (P<0.01). During the ischemia period, there was an increase in the QRS interval (P<0.05) and a reduction in the P wave and QT intervals (P<0.05 for both) in rats with epilepsy compared to control rats. During the reperfusion period, a significant reduction in the QT interval (P<0.01) was verified in the epilepsy group in relation to the control group. Our results indicate that rats submitted to an epilepsy model induced by pilocarpine presented electrical conductivity alterations of cardiac tissue, mainly during an AMI episode.
Descritores: Bacteriófago lambda/fisiologia
Escherichia coli/virologia
Proteínas Virais/metabolismo
-Sequência de Aminoácidos
Membrana Celular/metabolismo
Regulação Viral da Expressão Gênica/fisiologia
Dados de Sequência Molecular
Proteínas Virais/genética
Liberação de Vírus/fisiologia
Tipo de Publ: Research Support, N.I.H., Extramural
Responsável: BR1.1 - BIREME


  7 / 21 LILACS  
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Id: lil-678279
Autor: Memorias do Instituto Oswaldo Cruz; Martinez-Alvarez, Laura; Pina-Vazquez, Carolina; Zarco, Wilbert; Padilla-Noriega, Luis.
Título: The shift from low to high non-structural protein 1 expression in rotavirus-infected MA-104 cells
Fonte: Mem. Inst. Oswaldo Cruz;108(4):421-428, jun. 2013. graf.
Idioma: en.
Projeto: CONACyT; . CONACyT; . UNAM; . UNAM.
Resumo: A hallmark of group/species A rotavirus (RVA) replication in MA-104 cells is the logarithmic increase in viral mRNAs that occurs four-12 h post-infection. Viral protein synthesis typically lags closely behind mRNA synthesis but continues after mRNA levels plateau. However, RVA non-structural protein 1 (NSP1) is present at very low levels throughout viral replication despite showing robust protein synthesis. NSP1 has the contrasting properties of being susceptible to proteasomal degradation, but being stabilised against proteasomal degradation by viral proteins and/or viral mRNAs. We aimed to determine the kinetics of the accumulation and intracellular distribution of NSP1 in MA-104 cells infected with rhesus rotavirus (RRV). NSP1 preferentially localises to the perinuclear region of the cytoplasm of infected cells, forming abundant granules that are heterogeneous in size. Late in infection, large NSP1 granules predominate, coincident with a shift from low to high NSP1 expression levels. Our results indicate that rotavirus NSP1 is a late viral protein in MA-104 cells infected with RRV, presumably as a result of altered protein turnover.
Descritores: Proteínas do Capsídeo/metabolismo
Regulação Viral da Expressão Gênica
Rotavirus/metabolismo
Proteínas não Estruturais Virais/metabolismo
-Linhagem Celular
RNA Viral/genética
Rotavirus/fisiologia
Replicação Viral
Limites: Animais
Cobaias
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  8 / 21 LILACS  
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Texto completo SciELO Brasil
Beçak, W
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Id: lil-606544
Autor: Coimbra, E. C; Gomes, F. B; Campos, J. F; D’arc, M; Carvalho, J. C; Mariz, F. C; Jesus, A. L. S; Stocco, R. C; Beçak, W; Freitas, A. C.
Título: Production of L1 protein from different types of HPV in Pichia pastoris using an integrative vector
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;44(12):1209-1214, Dec. 2011. ilus, tab.
Idioma: en.
Resumo: Human papillomavirus (HPV) infection is the most common sexually transmitted disease in the world and is related to the etiology of cervical cancer. The most common high-risk HPV types are 16 and 18; however, the second most prevalent type in the Midwestern region of Brazil is HPV-33. New vaccine strategies against HPV have shown that virus-like particles (VLP) of the major capsid protein (L1) induce efficient production of antibodies, which confer protection against the same viral type. The methylotrophic yeast Pichia pastoris is an efficient and inexpensive expression system for the production of high levels of heterologous proteins stably using a wild-type gene in combination with an integrative vector. It was recently demonstrated that P. pastoris can produce the HPV-16 L1 protein by using an episomal vector associated with the optimized L1 gene. However, the use of an episomal vector is not appropriate for protein production on an industrial scale. In the present study, the vectors were integrated into the Pichia genome and the results were positive for L1 gene transcription and protein production, both intracellularly and in the extracellular environment. Despite the great potential for expression by the P. pastoris system, our results suggest a low yield of L1 recombinant protein, which, however, does not make this system unworkable. The achievement of stable clones containing the expression cassettes integrated in the genome may permit optimizations that could enable the establishment of a platform for the production of VLP-based vaccines.
Descritores: Alphapapillomavirus/imunologia
Proteínas do Capsídeo/biossíntese
Proteínas Oncogênicas Virais/biossíntese
Pichia/metabolismo
-Alphapapillomavirus/genética
Anticorpos Antivirais/imunologia
Proteínas do Capsídeo/genética
Transformação Celular Viral/fisiologia
Eletroforese em Gel de Poliacrilamida
Regulação Viral da Expressão Gênica
Proteínas Oncogênicas Virais/genética
Vacinas contra Papillomavirus/imunologia
Pichia/genética
Pichia/virologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  9 / 21 LILACS  
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Id: lil-571234
Autor: Martínez-Flores, Francisco; Jiménez-Orozco, Fausto Alejandro; Villegas-Castrejón, Hilda.
Título: Biología molecular de los vectores adenovirales / Molecular biology of adenoviral vectors
Fonte: Cir. & cir;74(6):483-493, nov.-dic. 2006. tab, ilus.
Idioma: es.
Resumo: La terapia con genes postula el uso terapéutico del DNA como una nueva alternativa de la biomedicina para el tratamiento de las enfermedades humanas. Todas las proteínas están codificadas en el DNA, y muchas enfermedades resultan de: a) la ausencia o expresión aberrante de uno o más genes; b) la ausencia de formas funcionales; c) alteraciones en su proceso de regulación, transporte o degradación. Por lo tanto, tales enfermedades pueden ser potencialmente tratadas, restableciendo la expresión de la proteína involucrada en las células afectadas. Sin embargo, para lograr una transferencia exitosa del material genético al sitio blanco y evitar la destrucción del DNA o del vehículo seleccionado antes de llegar al sitio de interés, se han desarrollado varios sistemas virales. Entre los virus más conocidos están: el virus del herpes simple, adenovirus tipo 5, virus adenoasociado y algunos retrovirus complejos (lentivirus). En este artículo se exponen las características biológicas, la manipulación genética y propiedades de los adenovirus, así como su empleo en la medicina actual como vectores para transferir genes y su potencial implicación en la terapia génica.

Gene therapy is based on the use of DNA as a therapeutic material as an alternative therapeutic tool for treatment of human diseases. All proteins are codified into the DNA and several diseases result from the absence or aberrant expression of one or related genes, absence of expression of functional proteins, and alterations for regulation process in transport and degradation mechanisms. In this regard, several diseases could be potentially treated through the expression of the normal form of the involved protein. However, the main objective is to achieve a successful genetic material delivery into the target site and avoid the destruction of DNA or the selected vehicle before arrival at the final destination. Several efficient viral gene transfer systems have been developed. Viral-mediated gene delivery for experimental models has been designed from herpes virus (HV), adenovirus (adenovirous), adeno-associated virus (AAV) and retroviruses (lentiviral vectors). In this review we will discuss the specific biological and cloning properties of adenoviral vectors as a gene transfer tool and potential medical implications for gene therapy.
Descritores: Doenças Genéticas Inatas/terapia
Mastadenovirus/genética
Vetores Genéticos/genética
-Regulação Viral da Expressão Gênica
Terapia Genética
Genoma Viral
Mastadenovirus/fisiologia
Mastadenovirus/ultraestrutura
Neoplasias do Colo do Útero/terapia
Transcrição Genética
Transdução Genética
Replicação Viral
Vetores Genéticos/uso terapêutico
Limites: Seres Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


  10 / 21 LILACS  
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Texto completo SciELO Brasil
Bonjardim, Claudio A
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Id: lil-547311
Autor: Andrade, Anderson A; Brasil, Bruno SAF; Pereira, Anna CTC; Ferreira, Paulo CP; Kroon, Erna G; Bonjardim, Cláudio A.
Título: Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
Fonte: Mem. Inst. Oswaldo Cruz;105(3):269-277, May 2010. ilus, graf.
Idioma: en.
Resumo: In this paper, we provide evidence that both the mRNA and protein levels of the cyclin-dependent kinase (CDK) inhibitor p21WAF1/CDK-interacting protein 1 (Cip1) increase upon infection of A431 cells with Vaccinia virus (VACV). In addition, the VACV growth factor (VGF) seems to be required for the gene expression because infection carried out with the mutant virus VACV-VGF- revealed that this strain was unable to stimulate its transcription. Our findings are also consistent with the notion that the VGF-mediated change in p21WAF1/Cip1 expression is dependent on tyrosine kinase pathway(s) and is partially dependent on mitogen-activated protein kinase/extracellular-signal regulated kinase 1/2. We believe that these pathways are biologically significant because VACV replication and dissemination was drastically affected when the infection was carried out in the presence of the relevant pharmacological inhibitors.
Descritores: /metabolismo
CYCLIN-DEPENDENT KINASE INHIBITOR PABORTION APPLICANTS/metabolismo
Vírus Vaccinia/fisiologia
-Linhagem Celular Tumoral
/genética
CYCLIN-DEPENDENT KINASE INHIBITOR PABORTION APPLICANTS/genética
Regulação Viral da Expressão Gênica/genética
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Proteínas Tirosina Quinases/genética
Proteínas Tirosina Quinases/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Transdução de Sinais/genética
Replicação Viral/genética
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde