Base de dados : LILACS
Pesquisa : G05.308.800 [Categoria DeCS]
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Id: biblio-1133439
Autor: Vieira, Sandra Elisabete; Bando, Silvia Yumi; Lauterbach, Gerhard da Paz; Moreira-Filho, Carlos Alberto.
Título: Human Leukocyte Transcriptional Response to SARS-CoV-2 Infection
Fonte: Clinics;75:e2078, 2020.
Idioma: en.
Projeto: FAPESP; . CNPq.
Descritores: Coronavirus
Leucócitos
-Pneumonia Viral/epidemiologia
Transcrição Genética
Ativação Transcricional
Infecções por Coronavirus/epidemiologia
Síndrome Respiratória Aguda Grave
Pandemias
Betacoronavirus
SARS-CoV-2
COVID-19
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Editorial
Responsável: BR1.1 - BIREME


  2 / 31 LILACS  
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Id: biblio-1124210
Autor: Sadeghi, Fatemeh; Kardar, Gholam Ali; Bolouri, Mohammad Reza; Nasri, Farzad; Sadri, Maryam; Falak, Reza.
Título: Overexpression of bHLH domain of HIF-1 failed to inhibit the HIF-1 transcriptional activity in hypoxia
Fonte: Biol. Res;53:25, 2020. tab, graf.
Idioma: en.
Projeto: Medical faculty, Iran University of Medical Sciences.
Resumo: BACKGROUND: Hypoxia inducible factor-1 (HIF-1) is considered as the most activated transcriptional factor in response to low oxygen level or hypoxia. HIF-1 binds the hypoxia response element (HRE) sequence in the promoter of different genes, mainly through the bHLH domain and activates the transcription of genes, especially those involved in angiogenesis and EMT. Considering the critical role of bHLH in binding HIF-1 to the HRE sequence, we hypothesized that bHLH could be a promising candidate to be targeted in hypoxia condition. METHODS: We inserted an inhibitory bHLH (ibHLH) domain in a pIRES2-EGFP vector and transfected HEK293T cells with either the control vector or the designed construct. The ibHLH domain consisted of bHLH domains of both HIF-1a and Arnt, capable of competing with HIF-1 in binding to HRE sequences. The transfected cells were then treated with 200 µM of cobalt chloride (CoCl2) for 48 h to induce hypoxia. Real-time PCR and western blot were performed to evaluate the effect of ibHLH on the genes and proteins involved in angiogenesis and EMT. RESULTS: Hypoxia was successfully induced in the HEK293T cell line as the gene expression of VEGF, vimentin, and ß-catenin were significantly increased after treatment of untransfected HEK293T cells with 200 µM CoCl2. The gene expression of VEGF, vimentin, and ß-catenin and protein level of ß-catenin were significantly decreased in the cells transfected with either control or ibHLH vectors in hypoxia. However, ibHLH failed to be effective on these genes and the protein level of ß-catenin, when compared to the control vector. We also observed that overexpression of ibHLH had more inhibitory effect on gene and protein expression of N-cadherin compared to the control vector. However, it was not statistically significant. CONCLUSION: bHLH has been reported to be an important domain involved in the DNA binding activity of HIF. However, we found that targeting this domain is not sufficient to inhibit the endogenous HIF-1 transcriptional activity. Further studies about the function of critical domains of HIF-1 are necessary for developing a specific HIF-1 inhibitor.
Descritores: Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Fator 1 Induzível por Hipóxia/metabolismo
Hipóxia/metabolismo
-Expressão Gênica
Ativação Transcricional/genética
Western Blotting
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Fator 1 Induzível por Hipóxia/genética
Células HEK293
Reação em Cadeia da Polimerase em Tempo Real
Hipóxia/genética
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


  3 / 31 LILACS  
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Id: biblio-1011422
Autor: Wei, Fan; Tang, Danfeng; Li, Zengqiang; Kashif, Muhammad Haneef; Khan, Aziz; Lu, Hai; Jia, Ruixing; Chen, Peng.
Título: Molecular cloning and subcellular localization of six HDACs and their roles in response to salt and drought stress in kenaf (Hibiscus cannabinus L)
Fonte: Biol. Res;52:20, 2019. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Modern Agro-industry Technology Research System.
Resumo: BACKGROUND: Histone acetylation is an important epigenetic modification that regulates gene activity in response to stress. Histone acetylation levels are reversibly regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). The imperative roles of HDACs in gene transcription, transcriptional regulation, growth and responses to stressful environment have been widely investigated in Arabidopsis. However, data regarding HDACs in kenaf crop has not been disclosed yet. RESULTS: In this study, six HDACs genes (HcHDA2, HcHDA6, HcHDA8, HcHDA9, HcHDA19, and HcSRT2) were isolated and characterized. Phylogenetic tree revealed that these HcHDACs shared high degree of sequence homology with those of Gossypium arboreum. Subcellular localization analysis showed that GFP-tagged HcHDA2 and HcHDA8 were predominantly localized in the nucleus, HcHDA6 and HcHDA19 in nucleus and cytosol. The HcHDA9 was found in both nucleus and plasma membranes. Real-time quantitative PCR showed that the six HcHDACs genes were expressed with distinct expression patterns across plant tissues. Furthermore, we determined differential accumulation of HcHDACs transcripts under salt and drought treatments, indicating that these enzymes may participate in the biological process under stress in kenaf. Finally, we showed that the levels of histone H3 and H4 acetylation were modulated by salt and drought stress in kenaf. CONCLUSIONS: We have isolated and characterized six HDACs genes from kenaf. These data showed that HDACs are imperative players for growth and development as well abiotic stress responses in kenaf.
Descritores: Estresse Fisiológico/fisiologia
Hibiscus/enzimologia
Histona Acetiltransferases/fisiologia
Secas
Histona Desacetilases/fisiologia
-Ativação Transcricional/fisiologia
Clonagem Molecular
Hibiscus/crescimento & desenvolvimento
Hibiscus/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


  4 / 31 LILACS  
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Id: biblio-839254
Autor: Wang, H; Shan, XB; Qiao, YJ.
Título: PDK2 promotes chondrogenic differentiation of mesenchymal stem cells by upregulation of Sox6 and activation of JNK/MAPK/ERK pathway
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(2):e5988, 2017. graf.
Idioma: en.
Resumo: This study was undertaken to clarify the role and mechanism of pyruvate dehydrogenase kinase isoform 2 (PDK2) in chondrogenic differentiation of mesenchymal stem cells (MSCs). MSCs were isolated from femurs and tibias of Sprague-Dawley rats, weighing 300-400 g (5 females and 5 males). Overexpression and knockdown of PDK2 were transfected into MSCs and then cell viability, adhesion and migration were assessed. Additionally, the roles of aberrant PDK2 in chondrogenesis markers SRY-related high mobility group-box 6 (Sox6), type ΙΙ procollagen gene (COL2A1), cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), type ΙX procollagen gene (COL9A2) and collagen type 1 alpha 1 (COL1A1) were measured by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The expressions of c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK) and extracellular regulated protein kinase (ERK) were measured. Overexpressing PDK2 promoted cell viability, adhesion and inhibited cell migration in MSCs (all P<0.05). qRT-PCR assay showed a potent increase in the mRNA expressions of all chondrogenesis markers in response to overexpressing PDK2 (P<0.01 or P<0.05). PDK2 overexpression also induced a significant accumulation in mRNA and protein expressions of JNK, p38MAPK and ERK in MSCs compared to the control (P<0.01 or P<0.05). Meanwhile, silencing PDK2 exerted the opposite effects on MSCs. This study shows a preliminary positive role and potential mechanisms of PDK2 in chondrogenic differentiation of MSCs. It lays the theoretical groundwork for uncovering the functions of PDK2 and provides a promising basis for repairing cartilage lesions in osteoarthritis.
Descritores: Condrogênese/fisiologia
Proteínas Quinases JNK Ativadas por Mitógeno/fisiologia
Sistema de Sinalização das MAP Quinases/fisiologia
Células-Tronco Mesenquimais/fisiologia
Proteínas Serina-Treonina Quinases/fisiologia
Fatores de Transcrição SOXE/fisiologia
-Diferenciação Celular
Ratos Sprague-Dawley
Ativação Transcricional
Regulação para Cima
Limites: Animais
Masculino
Feminino
Ratos
Responsável: BR1.1 - BIREME


  5 / 31 LILACS  
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Id: lil-763064
Autor: Wang, Chenghe; Chen, Zhong; Wu, Jia; Zhang, Yan; Hu, Jia; Ge, Qiangqiang; Wang, Tao; Yang, Weimin; Xu, Hua; Liu, Jihong; Ye, Zhangqun.
Título: Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD
Fonte: Int. braz. j. urol;41(4):764-772, July-Aug. 2015. graf.
Idioma: en.
Projeto: National Natural Science Foundation of PR China.
Resumo: ABSTRACTPurpose:RNA activation (RNAa) is a mechanism of gene activation triggered by promoter-targeted small double stranded RNAs (dsRNAs), also known as small activating RNAs (saRNAs). Myogenic regulatory factor MyoD is regarded as the master activator of myogenic differentiation cascade by binding to enhancer of muscle specific genes. Stress urinary incontinence (SUI) is a condition primarily resulted from urethral sphincter deficiency. It is thus expected that by promoting differentiation of adipose-derived stem cells (ADSCs) into myoblasts by activating MyoD gene through RNAa may offer benefits to SUI.Materials and Methods:Rats ADSCs were isolated, proliferated in vitro, and identified by flow cytometry. Purified ADSCs were then transfected with a MyoD saRNA or control transfected. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to detect MyoD mRNA and protein expression, respectively. Immunocytochemical staining was applied to determine the expression of desmin protein in transfected cells. Cell viability was measured by using CellTiter 96® AQueous One Solution Cell Proliferation Assay kit.Results:Transfection of a MyoD saRNA (dsMyoD) into ADSCs significantly induced the expression of MyoD at both the mRNA and protein levels, and inhibited cell proliferation. Desmin protein expression was detected in dsMyoD treated ADSCs 2 weeks later.Conclusion:Our findings show that RNAa mediated overexpression of MyoD can promote transdifferentiation of ADSCs into myoblasts and may help treat stress urinary incontinence (SUI)–a condition primarily resulted from urethral sphincter deficiency.
Descritores: Tecido Adiposo/citologia
Diferenciação Celular/genética
Desmina/metabolismo
Proteína MyoD/genética
Mioblastos/citologia
RNA de Cadeia Dupla
Células-Tronco/citologia
-Western Blotting
Sobrevivência Celular
Citometria de Fluxo
Expressão Gênica
Imuno-Histoquímica
Proteína MyoD/metabolismo
Mioblastos/metabolismo
Cultura Primária de Células
Regiões Promotoras Genéticas/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Células-Tronco/metabolismo
Transfecção
Ativação Transcricional/fisiologia
Uretra/patologia
Incontinência Urinária por Estresse/genética
Incontinência Urinária por Estresse/metabolismo
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 31 LILACS  
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Id: lil-748256
Autor: Gomaa, Ola M.; Momtaz, Osama A..
Título: Copper induction and differential expression of laccase in Aspergillus flavus
Fonte: Braz. j. microbiol;46(1):285-292, 05/2015. tab, graf.
Idioma: en.
Resumo: Aspergillus flavus was isolated from soil and exhibited laccase activity under both constitutive and copper induced conditions. Spiking the medium with 1 mM copper sulfate resulted in an increase in the activity which reached 51.84 U/mL, a distinctive protein band was detected at 60 kDa. The extracellular enzyme was purified 81 fold using gel filtration chromatography and resulted in two different laccase fractions L1 and L2, the latter had a higher enzymatic activity which reached 79.57 U/mL and specific activity of 64.17 U/μg protein. The analysis of the spectrum of the L2 fraction showed a shoulder at 330 nm which is characteristic for T2/T3 copper centers; both copper and zinc were detected suggesting that this is an unconventional white laccase. Primers of laccase gene were designed and synthesized to recover specific gene from A. flavus. Sequence analysis indicated putative laccase (Genbank ID: JF683612) at the amino acid level suggesting a close identity to laccases from other genera containing the copper binding site. Decolorization of textile waste water under different conditions showed possible application in bioremediation within a short period of time. The effect of copper on A. flavus was concentration dependent.
Descritores: Aspergillus flavus/efeitos dos fármacos
Aspergillus flavus/enzimologia
Cobre/metabolismo
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Lacase/biossíntese
Ativação Transcricional/efeitos dos fármacos
-Aspergillus flavus/genética
Aspergillus flavus/isolamento & purificação
Cromatografia em Gel
Meios de Cultura/química
DNA Fúngico/genética
Eletroforese em Gel de Poliacrilamida
Resíduos Industriais
Lacase/química
Lacase/isolamento & purificação
Dados de Sequência Molecular
Peso Molecular
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Microbiologia do Solo
Análise Espectral
Purificação da Água
Responsável: BR1.1 - BIREME


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Id: lil-746871
Autor: Rocha, Natália Pereira da.
Título: Identificação do polimorfismo no receptor para TNF-alfa (TNFR1 posição +36A/G) e sua expressão em lesões de colo uterino / Identification of TNF-alfa polymorphism (TNFR1+36A/G) and its expression in uterine cervix lesions.
Fonte: Rio de Janeiro; s.n; 2013. xv,86 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Instituto de Pesquisa Clínica Evandro Chagas para obtenção do grau de Mestre.
Resumo: A infecção pelo HPV é o principal fator de risco para o desenvolvimento do carcinoma cervical. Contudo, a maioria das mulheres permanece assintomática, desenvolvendo lesões intraepiteliais de baixo e alto grau. Estudos demonstram que polimorfismos nas regiões promotoras de genes de citocinas estão associados com a gravidade de muitas doenças infecciosas. Dentre estas, podemos citar a citocina pró-inflamatória TNF-alfa, onde suas atividades celulares são mediadas pela interação entre a citocina e seus receptores (TNFR1 e R2). Assim, tivemos como objetivo neste estudo, identificar polimorfismos dos receptores de TNF-alfa associar ao aparecimento e progressão da lesão cervical, além de avaliar a expressao da proteína TNFR1 em lesoes cervicais. As técnicas de PCR e RFLP foram realizadas para identificar o polimorfismo de base única (SNP) do gene TNFR1 na posição +36A/G. Para a expressão da proteína foi realizada a imunohistoquimica em fragmentos de tecido parafinado provenientes de lesões cervicais e histerectomias (controle)Foram incluídas 365 mulheres neste estudo, sendo 183 pacientes e 182 controles. O genótipo AA TNFR1+36A/G foi associado com a proteção ao desenvolvimento de lesões de baixo grau (p=0,03) Para a análise da expressão de TNFR1, foram selecionadas 46 mulheres (17 controles, 15 HSIL e 14 LSIL). Foi observado um aumento na expressão deste receptor em células inflamatórias no corion de mulheres portadores de HSIL quando comparadas com o grupo controle (p=0,001). Ao associarmos os genótipos com a expressão da proteina, foi observado que portadoras de TNFR1+36AA não apresentam alterações na expressão TNFR1 independente do grau de lesão cervical. Assim, a presença de TNFR1 pode estar sendo modulada negativamente pela infecção por HPV e este marcador pode ser utilizado como indicador para a progressão de lesões pré-malignas cervicais.

HPV infection is the main risk factor for development of cervical carcinoma. However,most women remain asymptomatic, developing high grade and low intraepitheliallesions. Studies demonstrated that polymorphisms in promoter regions of cytokinesgenes are associated with the severity of many infectious diseases. Among these, wemention the proinflammatory cytokine TNF-alfa, where their cellular activities aremediated by cytokine and their receptors (TNFR1 and R2) interaction. Thus, in thisstudy, we have the aim to identify TNF-alfa receptors polymorphisms and associate withthe onset and progression of cervical lesions, and to evaluate the TNFR1 expression incervical lesions. PCR and RFLP techniques were performed to identify the single basepolymorphism (SNP) TNFR1 gene at position +36 A/G. For protein expression, it wasperformed immunohistochemistry on paraffin tissue fragments from cervical lesionsand hysterectomies (control). Three hundred and sixty five women were included, 183patients and 182 controls. The AA genotype TNFR1 +36 A /G was associated withprotection from developing low-grade lesions (p = 0.03) for the analysis of TNFR1expression, we selected 46 women (17 controls, 15 HSIL and 14 LSIL). It wasobserved an increase in the receptor expression on inflammatory cells from corion ofHSIL women when compared with the control group (p = 0.001). By associatinggenotypes with expression of the protein, it was observed that carriers of TNFR1 +36AA did not show changes in the TNFR1expression, independent cervical lesion degree.Thus, the TNFR1 presence may be negatively being modulated by HPV infection andthis marker can be used as an indicator of premalignant cervical lesions progression.
Descritores: Neoplasia Intraepitelial Cervical
Polimorfismo Genético
Ativação Transcricional
Fator de Necrose Tumoral alfa
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas


  8 / 31 LILACS  
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Id: lil-742546
Autor: Marzi, Marta; Weitz, Darío; Avila, Aylén; Molina, Gabriel; Caraballo, Lucía; Piskulic, Laura.
Título: Efectos adversos cardíacos de la domperidona en pacientes adultos: revisión sistemática / Cardiac adverse effects of domperidone in adult patients: a systematic review
Fonte: Rev. méd. Chile;143(1):14-21, ene. 2015. ilus, tab.
Idioma: es.
Resumo: Background: Domperidone is widely prescribed in patients with gastrointestinal disorders but some cardiac adverse effects have been recently reported. Aim: To evaluate the risk of QT prolongation, ventricular arrhythmias and sudden cardiac death associated with the use of oral domperidone in adults without cancer. Material and Methods: Systematic searches in MEDLINE, LILACS, SciELO, the Cochrane Library and regulatory agencies websites were performed, followed by a manual search of cited references. The search strategy consisted of combining free and indexed text words without any date or language restriction. Results: Three case-control studies met the inclusion criteria; none of them evaluated QT interval prolongation. With low risk of bias, each study quantified the risk of ventricular arrhythmia or sudden cardiac death (VA/SCD). The odds ratios for these events in these studies were 4.7 (95% confidence interval (CI): 1.4-16), 1.59 (95% CI: 1.28-1.98) and 11.02 (95% CI: 2.02-62.3) respectively. A significantly increased risk was observed in patients older than 60 years of age or receiving doses > 30 mg/day. Conclusions: Heterogeneity between selected studies did not allow the computation of a summary measure. However, evidence was found that an increased risk of VA/SCD is associated with the use of oral domperidone in adults.
Descritores: Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
Neoplasias da Mama/tratamento farmacológico
Diterpenos/administração & dosagem
Compostos de Epóxi/administração & dosagem
Paclitaxel/administração & dosagem
-Protocolos de Quimioterapia Combinada Antineoplásica/química
Apoptose/efeitos dos fármacos
Neoplasias da Mama/genética
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Linhagem Celular Tumoral
Sinergismo Farmacológico
Diterpenos/química
Compostos de Epóxi/química
Lactonas/administração & dosagem
Lactonas/química
Camundongos Nus
Estresse Oxidativo/efeitos dos fármacos
Poli(ADP-Ribose) Polimerases/genética
Poli(ADP-Ribose) Polimerases/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Relação Estrutura-Atividade
Carga Tumoral
Ativação Transcricional/efeitos dos fármacos
Ensaios Antitumorais Modelo de Xenoenxerto
Limites: Animais
Feminino
Humanos
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  9 / 31 LILACS  
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Id: lil-731050
Autor: Mello, Victor Villaça Cardoso de; Barbosa, André Cavalcante da Silva; Morais, Mariana Pacheco Lima de Assis; Gomes, Simone Guimarães Farias; Vasconcelos, Márcia Maria Vendiciano Barbosa; Caldas Júnior, Arnaldo de França.
Título: Temporomandibular Disorders in a Sample Population of the Brazilian Northeast
Fonte: Braz. dent. j;25(5):442-446, Sep-Oct/2014. tab, graf.
Idioma: en.
Resumo: Temporomandibular disorder (TMD) is a common condition. This study is part of a research group and it investigated the prevalence of TMD and myofascial pain and its association with gender, age and socioeconomic class. The sample comprised 100 subjects, aged 15 to 70, users of the Family Health Units' services, in the city of Recife, PE, Brazil. The TMD degree was evaluated using the Research Diagnostic Criteria for TMD and socioeconomic class by the Economic Classification Criteria Brazil. Categorical variables were analyzed by chi-square test for proportions and Fisher's exact test for 2x2 tables, and binary logistic analysis to track the relationship between the independent and dependent variables. According to the results, 42% of the subjects had TMD and 14% myofascial pain. No statistically significant association could be found between TMD and gender or socioeconomic class, but it was found to have statistically significant association with age, and myofascial pain was associated with socioeconomic class. Considering that the results of the present study should be confirmed by further studies and the fact that this was a pilot study, the prevalence must be analyzed with caution.

Disfunção temporomandibular (DTM) é uma condição comum. Este estudo é parte de um grupo de pesquisa e investigou a prevalência de DTM e dor miofascial e suas associações com sexo, idade e classe socioeconômica. A amostra foi composta por 100 indivíduos, com idades entre 15 e 70 anos, usuários das Unidades de Saúde da Família, na cidade de Recife, PE. O grau de DTM foi avaliado usando os Critérios de Diagnósticos Científicos em DTM, e classe socioeconômica com o Critério de Classificação Econômica Brasil. As variáveis categóricas foram analisadas pelo teste do qui-quadrado para proporções e teste exato de Fisher para tabelas 2x2, e a análise logística binária para traçar a relação entre as variáveis independentes e dependentes. De acordo com os resultados, 42% dos indivíduos tinham DTM e 14% dor miofascial. Não houve associação estatisticamente significativa entre DTM e sexo ou classe socioeconômica, mas houve associação estatisticamente significativa com a idade e a dor miofascial foi associada com a classe socioeconômica. Considerando-se que os resultados do presente estudo devam ser confirmados em outros estudos e por causa de sua natureza piloto, a prevalência deve ser analisada com cautela.
Descritores: Regulação Bacteriana da Expressão Gênica
Óperon
Fenilacetatos/metabolismo
Pseudomonas putida/genética
Pseudomonas putida/crescimento & desenvolvimento
-Técnicas de Cultura de Células
Divisão Celular
Meios de Cultura
Carbono/metabolismo
Coenzima A Ligases/biossíntese
Coenzima A Ligases/genética
Compostos Inorgânicos/metabolismo
Oxigenases/biossíntese
Oxigenases/genética
Pseudomonas putida/metabolismo
Reprodutibilidade dos Testes
Ácido Succínico/metabolismo
Ativação Transcricional
Responsável: BR1.1 - BIREME


  10 / 31 LILACS  
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Id: lil-665796
Autor: Brazilian Journal of Medical and Biological Research; Zhang, Yafei; Zhang, Bicheng; Zhang, Anran; Li, Xiaohua; Liu, Jian; Zhao, Jie; Zhao, Yong; Gao, Jianfei; Fang, Dianchun; Rao, Zhiguo.
Título: IL-6 upregulation contributes to the reduction of miR-26a expression in hepatocellular carcinoma cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;46(1):32-38, 11/jan. 2013. graf.
Idioma: en.
Projeto: National Foundation of Natural Sciences of China; . China Postdoctoral Science Foundation; . Natural Science Foundation of Hubei Province of China.
Resumo: A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be a predictor of survival up to 5 years for hepatocellular carcinoma patients (log-rank test, P < 0.05). We observed that the serum IL-6 concentration was inversely correlated with miR-26a expression in cancerous tissues (Pearson correlation test, r = -0.651, P < 0.01). Furthermore, by in vitro experiments with HepG2 cells, we showed that IL-6 stimulation can lead to miR-26a suppression via c-Myc activation, whereas in normal hepatocyte LO2 cells incubation with IL-6 had no significant effect on miR-26a expression. Taken together, these results indicate that miR-26a reduction in hepatocellular carcinoma might be due to IL-6 upregulation.
Descritores: Carcinoma Hepatocelular/metabolismo
/metabolismo
INTERLEUKIN-ABDOMEN, ACUTE/metabolismo
Neoplasias Hepáticas/metabolismo
MicroRNAs/metabolismo
-Estudos de Casos e Controles
Carcinoma Hepatocelular/genética
Regulação Neoplásica da Expressão Gênica
Imuno-Histoquímica
/genética
INTERLEUKIN-ABDOMEN, ACUTE/genética
Neoplasias Hepáticas/genética
MicroRNAs/genética
MicroRNAs/fisiologia
Recidiva
Ativação Transcricional/genética
Regulação para Cima
Limites: Adulto
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde