Base de dados : LILACS
Pesquisa : G05.360.337.500 [Categoria DeCS]
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Texto completo SciELO Chile
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Id: lil-551371
Autor: Chibeu, Andrew; Lavigne, Rob; Mattheus, Wesley; Matthijs, Sandra; Cornelis, Pierre; Volckaert, Guido.
Título: Genetic and physical map of broad host range cosmid pRG930cm
Fonte: Electron. j. biotechnol;12(2):10-11, Apr. 2009. ilus, tab.
Idioma: en.
Resumo: We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries.
Descritores: Azorhizobium caulinodans/genética
Escherichia coli/genética
Agrobacterium tumefaciens/genética
-Resistência ao Cloranfenicol
Engenharia Genética
Tipo de Publ: Relatório Técnico
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Brasil
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Id: lil-197224
Autor: Tanaka, Manani; Tanaka, Tomoo; Inazawa, Johji; Nagafuchi, Shigeo; Matsui, Youji; Kaukas, Anne; Johnston, David A.
Título: Proceedings of the schistosome genome project
Fonte: Mem. Inst. Oswaldo Cruz;92(6):829-34, Nov.-Dec. 1997. ilus.
Idioma: en.
Conferência: Apresentado em: Parasite Genome Projects: Strategies and Methods, Buenos Aires, Nov. 13-24, 1995.
Resumo: "The host-parasite relationship" is a vast and diverse research field which, despite huge human and financial input over many years, remains largely shrouded in mystery. Clearly, the adaptation of parasites to their different host species, and to the different environomental stresses that they represent, depends on interactions with, and responses to, various molecules of host and/or parasite origin. The schistosome genome project is a primary strategy to reach the goal; this systematic research project has successfully developed novel technologies for qualitative and quantitative characterization of schistosome genes and genome organization by extensive international collaboration between top quality laboratories. Schistosomes are a family of parasitic blood flukes (Phylum Platyhelminthes), which have seven pairs of autosomal chromosomes and one pair of sex chromosomes (ZZ for a male worm an ZW for a female), of a haploid genome size of 2.7X10 8 base pairs (Simpson et al. 1982). Schistosomes are ideal model organisms for the development of genome mapping strategies since they have a small genome size comparable to that of well-characterized model organisms such as Caenorhabditis elegans (100 Mb) and Drosophila (165Mb), and contain functional genes with a high level of homology to the host mammalian genes. Here we summarize the current progress in the schistosome genome project, the information of 3.047 transcribed genes (Expresses Sequence Tags; EST), complete sets of cDNA and genomic DNA libraries (including YAC and cosmid libraries) with a mapping technique to the well defined schistosome chromosomes. The schistosome genome project will further identify and charaterize the key molecules that are responsable for host-parasite adaptation, i.e., successful growth developement, maturation and reproduction of the parasite within its host in the near future.
Descritores: Genoma
-Cromossomos Artificiais de Levedura
Hibridização In Situ
Limites: Animais
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas

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Id: lil-138276
Autor: Turco, S; Descoteaux, A; Ryan, K; Garraway, L; Beverley, S.
Título: Isolation of virulence genes directing GPI synthesis by functional complementation of Leishmania
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;27(2):133-8, Feb. 1994. ilus.
Idioma: en.
Conferência: Apresentado em: GPI-Anchor Proteins: Structure and Function - 1994 Meeting, Angra dos Reis, 20-26 Feb. 1994.
Resumo: Recent advances in molecular genetics of Leishmania parasites prompted us to develop methods of functional genetic complementation in Leishmania and apply them to the isolation of genes involved in the biosynthesis of the virulence determinant LPG, an abundant GPI-anchored polysaccharide. LPG1, the gene product identified by complementation of our R2D2 LPG- mutant, may be a glycosyltransferase responsible for the addition of galactofuranose to the nascent chain. As galactofuranose is not found in mammalian cells, inhibition of the addition of this sugar could be exploited for chemotherapy. Overall, the success of the functional complementation approach opens the way to the identification of a variety of genes involved in pathogenesis and parasitism
Descritores: Fosfatidilinositóis/biossíntese
Teste de Complementação Genética
Leishmania donovani/genética
Sequência de Aminoácidos
Anticorpos Monoclonais
Sequência de Bases
DNA de Protozoário/genética
DNA de Protozoário/metabolismo
Biblioteca Gênica
Leishmania donovani/patogenicidade
Dados de Sequência Molecular
Limites: Animais
Responsável: BR26.1 - Biblioteca Central

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