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Id: biblio-1047375
Autor: Li, Shanshan; Qin, Kun; Li, Huaying; Guo, Jin; Li, Dejin; Liu, Fang; Tan, Zhilei; Yan, Wei; Qu, Shuling; Zhao, Huabing.
Título: Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6
Fonte: Electron. j. biotechnol;34:83-90, july. 2018. tab, ilus, graf.
Idioma: en.
Projeto: Tianjin Research Program of Application Foundation and Advanced Technology; . National Natural Science Foundation of China.
Resumo: Background: Although the functional redundancy of catechol 1,2-dioxygenase (C12O) genes has been reported in several microorganisms, limited enzymes were characterised, let alone the advantage of the coexistence of the multiple copies of C12O genes. Results: In this study, four novel C12O genes, designated catA, catAI, catAII and catAIII, in the naphthalene-degrading strain Pseudomonas putida ND6, were cloned and characterised. Phylogenetic analysis of their deduced amino acid sequences revealed that the four C12O isozymes each formed independent subtrees, together with homologues from other organisms. All four enzymes exhibited maximum activity at pH 7.4 and higher activity in alkaline than in acidic conditions. Furthermore, CatA, CatAI and CatAIII were maximally active at a temperature of 45°C, whereas a higher optimum temperature was observed for CatAII at a temperature of 50°C. CatAI exhibited superior temperature stability compared with the other three C12O isozymes, and kinetic analysis indicated similar enzyme activities for CatA, CatAI and CatAII, whereas that of CatAIII was lower. Significantly, among metal ions tested, only Cu2+ substantially inhibited the activity of these C12O isozymes, thus indicating that they have potential to facilitate bioremediation in environments polluted with aromatics in the presence of metals. Moreover, gene expression analysis at the mRNA level and determination of enzyme activity clearly indicated that the redundancy of the catA genes has increased the levels of C12O. Conclusion: The results clearly imply that the redundancy of catA genes increases the available amount of C12O in P. putida ND6, which would be beneficial for survival in challenging environments.
Descritores: Pseudomonas putida/enzimologia
Pseudomonas putida/genética
Catecol 1,2-Dioxigenase/genética
-Temperatura
Biodegradação Ambiental
Clonagem Molecular
Catecol 1,2-Dioxigenase/análise
Catecol 1,2-Dioxigenase/metabolismo
Genes Bacterianos
Concentração de Íons de Hidrogênio
Isoenzimas
Metais
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Id: biblio-1177447
Autor: Bai, Yu; Li, Wenliang; Xu, Guangyu; Cui, Guihua.
Título: A bioinformatics approach revealed the transcription factors of Helicobacter pylori pathogenic genes and their regulatory network nodes
Fonte: Electron. j. biotechnol;45:53-59, May 15, 2020. tab, ilus.
Idioma: en.
Projeto: Department of Education of Jilin Province; . Jilin Province Traditional Chinese Medicine Science and Technology Project; . Jilin Province Health and Family Planning Commission; . Jilin Science and Technology Innovation Development Plan Project.
Resumo: BACKGROUND: Helicobacter pylori is a chronic pathogenic bacteria that causes gastric mucosal damage through various host-related and pathogen-related factors. Thus, a single gene research cannot fully explain its pathogenicity. PURPOSE OF STUDY: It is necessary to establish a Helicobacter pylori pathogenic gene transcription factor regulatory network (TFRN) and study its central nodes. RESULTS: The expression data of Helicobacter pylori pathogenic genes were obtained through GEO Datasets of NCBI. The genes were screened using linear model-empirical Bayesian statistics in R language Limma package combined with the conventional t-test; the results identified 1231 differentially expressed genes. The functional analysis (gene ontology-analysis) and signal pathway analysis (pathway-analysis) of differentially expressed genes were performed using the DAVID and KEGG databases, respectively. The pathogenic gene regulatory network was constructed by integrating transcriptional regulatory element database (TRED); the disease-related analysis of the pathogenic genes was conducted using the DAVID annotation tool. Five pathogenic genes (Nos2, Il5, Colla1, Tnf, and Nfkb1) and their transcription factors (Jun, Cebpa, Egrl, Ppara, and Il6) were found to suppress the host immune function and enhance the pathogenicity of Helicobacter pylori by regulating the host immune system. CONCLUSIONS: This effect was largely mediated via three signaling pathways: Tnf pathway, PI3K Akt pathway, and Jak­STAT pathway. The pathogenicity of Helicobacter pylori is closely related to the body's immune and inflammatory system. A better understanding of the correlation of the pathogenic factors with the host immune and inflammatory factors may help to determine the precise pathogenic mechanism of H. pylori infection.
Descritores: Helicobacter pylori/genética
Helicobacter pylori/patogenicidade
Biologia Computacional
-Fatores de Transcrição
Citocinas
Fatores de Virulência
Gastrite/imunologia
Gastrite/microbiologia
Genes Bacterianos
Sistema Imunitário
Inflamação
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-899771
Autor: Aguayo-Reyes, Alejandro; Quezada-Aguiluz, Mario; Mella, Sergio; Riedel, Gisela; Opazo-Capurro, Andrés; Bello-Toledo, Helia; Domínguez, Mariana; González-Rocha, Gerardo.
Título: Bases moleculares de la resistencia a meticilina en Staphylococcus aureus / Molecular basis of methicillin-resistance in Staphylococcus aureus
Fonte: Rev. chil. infectol;35(1):7-14, 2018. tab, graf.
Idioma: es.
Projeto: FONDECYT.
Resumo: Resumen Desde el inicio de la era antimicrobiana se han ido seleccionando gradualmente cepas de Staphylococcus aureus resistentes a antimicrobianos de amplio uso clínico. Es así como en 1960 se describen en Inglaterra las primeras cepas resistentes a meticilina, y algunos años después son informadas en hospitales de Chile. Actualmente, S. aureus resistente a penicilinas antiestafilocóccicas es endémico en los hospitales de nuestro país y del mundo, siendo responsable de una alta morbimortalidad. La resistencia es mediada habitualmente por la síntesis de una nueva transpeptidasa, denominada PBP2a o PBP2' que posee menos afinidad por el β-lactámico, y es la que mantiene la síntesis de peptidoglicano en presencia del antimicrobiano. Esta nueva enzima se encuentra codificada en el gen mecA, a su vez inserto en un cassette cromosomal con estructura de isla genómica, de los cuales existen varios tipos y subtipos. La resistencia a meticilina se encuentra regulada, principalmente, por un mecanismo de inducción de la expresión del gen en presencia del β-lactámico, a través de un receptor de membrana y un represor de la expresión. Si bien se han descrito mecanismos generadores de resistencia a meticilina mec independientes, son categóricamente menos frecuentes.

Staphylococcus aureus isolates resistant to several antimicrobials have been gradually emerged since the beginning of the antibiotic era. Consequently, the first isolation of methicillin-resistant S. aureus occurred in 1960, which was described a few years later in Chile. Currently, S. aureus resistant to antistaphylococcal penicillins is endemic in Chilean hospitals and worldwide, being responsible for a high burden of morbidity and mortality. This resistance is mediated by the expression of a new transpeptidase, named PBP2a or PBP2', which possesses lower affinity for the β-lactam antibiotics, allowing the synthesis of peptidoglycan even in presence of these antimicrobial agents. This new enzyme is encoded by the mecA gene, itself embedded in a chromosomal cassette displaying a genomic island structure, of which there are several types and subtypes. Methicillin resistance is mainly regulated by an induction mechanism activated in the presence of β-lactams, through a membrane receptor and a repressor of the gene expression. Although mec-independent methicillin resistance mechanisms have been described, they are clearly infrequent.
Descritores: Proteínas de Bactérias/genética
Estruturas Genéticas/genética
Proteínas de Ligação às Penicilinas/genética
Staphylococcus aureus Resistente à Meticilina/genética
-Proteínas de Bactérias/efeitos dos fármacos
Estrutura Molecular
Cromossomos Bacterianos/efeitos dos fármacos
Proteínas de Ligação às Penicilinas/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Genes Bacterianos/efeitos dos fármacos
Meticilina/farmacologia
Meticilina/química
Antibacterianos/farmacologia
Antibacterianos/química
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: lil-792801
Autor: Shahraki-Zahedani, Shahram; Rigi, Shahnaz; Bokaeian, Mohammad; Ansari-Moghaddam, Alireza; Moghadampour, Mehdi.
Título: First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran
Fonte: Rev. Soc. Bras. Med. Trop;49(4):441-445, July-Aug. 2016. tab, graf.
Idioma: en.
Resumo: Abstract: INTRODUCTION: Extended-spectrum beta-lactamases (ESBLs) are bacterial enzymes capable of hydrolyzing beta-lactams. The aim of this study was to describe the prevalence of TEM- and SHV-type ESBL-producing Klebsiella pneumoniae strains in Zahedan, Southeast Iran. METHODS: A total of 170 non-repetitive K. pneumoniae strains were collected from patients referred to three teaching hospitals of Zahedan. Antibiotic susceptibility testing was determined for 17 antibiotics using the Kirby-Bauer disc diffusion method. The frequency of ESBL-producing strains was calculated, and minimum inhibitory concentrations of ESBL-producing strains were determined for cefotaxime, ceftazidime, ceftriaxone, and cefpodoxime. The presence of bla TEM and bla SHV genes was tested in all ESBL-producing strains using polymerase chain reaction and DNA sequencing. RESULTS: Among the 170 K. pneumoniae clinical isolates, 55 (32.4%) were ESBL producers; 92.7% (n=51) and 72.7% (n=40) of the isolates carried the bla SHV and bla TEM genes, respectively, and 67.3% (n=37) carried both genes. The sequencing results showed that all bla TEM types were bla TEM-1, except for two isolates that were bla TEM-104. The bla SHV types were bla SHV-1, bla SHV-11, bla SHV-12, bla SHV-99, bla SHV-108, and bla SHV-110. CONCLUSIONS: The percentage of bla TEM and bla SHV among ESBL-producing K. pneumoniae isolates from Zahedan is relatively high, indicating the need for further surveillance and consideration in antibiotic use. To the best of our knowledge, this is the first report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-type ESBLs among clinical isolates of K. pneumoniae from Iran, and TEM-1, SHV-1, SHV-11, and SHV-12 appear to be the dominant ESBLs in this region.
Descritores: Proteínas de Bactérias/genética
beta-Lactamases/biossíntese
DNA Bacteriano/genética
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/genética
Antibacterianos/farmacologia
-Fenótipo
Infecções por Klebsiella/microbiologia
Análise de Sequência de DNA
Farmacorresistência Bacteriana Múltipla/genética
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Genes Bacterianos
Genótipo
Irã (Geográfico)
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-959424
Autor: García, José; Martínez, Dianny; Caña, Luisa; González, Diorelis; Rodríguez, Lucy; Rodulfo, Hectorina; Donato, Marcos De; Guzmán, Militza.
Título: Genes qnr en Enterobacteriaceae aisladas en un hospital de Venezuela / qnr genes in Enterobacteriaceae isolated from at a hospital in Venezuela
Fonte: Rev. chil. infectol;35(2):147-154, abr. 2018. tab, graf.
Idioma: es.
Projeto: Misión Ciencia.
Resumo: Resumen Introducción: La resistencia de enterobacterias a quinolonas se ha difundido por el mundo, fenómeno presente también en Venezuela. El mecanismo de esta resistencia pudiera estar mediado por genes incluidos en el cromosoma bacteriano o transmitirse en el interior de plásmidos. Objetivo: Evaluar la resistencia a quino-lonas, codificada por genes qnr, presentes en cepas de enterobacterias, aisladas en el Hospital Universitario de Cumaná, Venezuela. Métodos: A las cepas obtenidas se les realizaron pruebas de susceptibilidad antimicrobiana a quinolonas, β-lactámicos y aminoglucósidos. La presencia del gen qnr se determinó por RPC. Las enterobacterias portadoras del gen qnr fueron sometidas al proceso de conjugación bacteriana para comprobar su capacidad de transferencia. A las transconjugantes obtenidas se les realizó pruebas de susceptibilidad antimicrobiana y RPC para comprobar la transferencia de los genes. Resultados: Se encontraron elevados porcentajes de resistencia antimicrobiana a quinolonas y betalactámicos. El 33,6% de las cepas eran portadoras del gen qnrB, y 0,9% del gen qnrA. Se obtuvieron 23 cepas transconjugantes; de éstas, 20 portaban el gen qnrB, no se observó la presencia de qnrA. Discusión: En conclusión, el elevado porcentaje de genes qnr encontrado en las enterobacterias aisladas, y comprobada la presencia de éstos en plásmidos transferibles, complica la aplicación de tratamientos basados en quinolonas y fluoroquinolonas, por lo que es recomendable el uso racional de estos antimicrobianos, y proponer la rotación de la terapia antimicrobiana, a fin de evitar la selección de cepas resistentes.

Background: Enterobacteria resistant to quinolones is increasing worldwide, including Venezuela. The mechanism for this resistance could be due to genes included in the chromosome or in transmissible plasmids. Aim: To evaluate the resistance to quinolones, coded by qnr genes present in enterobacteria species, isolated in the University Hospital of Cumana, Venezuela. Methods: Antimicrobial susceptibility tests to quinolones, beta-lactams and aminoglycosides were carried out to all the isolates. The presence of qnr genes were determined by PCR. The isolates carrying the qnr genes were used for bacterial conjugation tests to determine the presence of transferable plasmids. Antimicrobial susceptibility tests and PCR were carried out in the transconjugants to verify the transfer of the genes. Results: High levels of antimicrobial resistance to quinolones and beta-lactams were found among the isolates. We found that 33.6% of the isolates carry the qnrB gene and 0.9% qnr A gene. Of the 23 transconjugants, 20 showed to have qnrB gene, but none qnrA. Discussion: We concluded that the high frequency of qnr genes found in the enterobacteria isolates and their presence on transferable plasmids, complicate the use of quinolones for the treatment of bacterial infections, thus, a treatment plan should be designed with the rational use and the rotation of different types of antimicrobials, in order to avoid the selection of increasingly resistant strains.
Descritores: Plasmídeos
Quinolonas/farmacologia
Resistência beta-Lactâmica/genética
Farmacorresistência Bacteriana/genética
Enterobacteriaceae/genética
Infecções por Enterobacteriaceae/genética
Bactérias Gram-Negativas/genética
Antibacterianos/farmacologia
-Venezuela
beta-Lactamases/genética
DNA Bacteriano/genética
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Proteínas de Escherichia coli
Enterobacteriaceae/isolamento & purificação
Infecções por Enterobacteriaceae/microbiologia
Genes Bacterianos
Bactérias Gram-Negativas/classificação
Hospitais Universitários
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-896964
Autor: Amirkamali, Sahar; Naserpour-Farivar, Taghi; Azarhoosh, Khadijeh; Peymani, Amir.
Título: Distribution of the bla OXA, bla VEB-1, and bla GES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran
Fonte: Rev. Soc. Bras. Med. Trop;50(3):315-320, May-June 2017. tab.
Idioma: en.
Projeto: Qazvin University of Medical Sciences.
Resumo: Abstract INTRODUCTION: Pseudomonas aeruginosa is one of the most common nosocomial pathogens. The emergence of extended spectrum β-lactamases (ESBLs) has been increasingly reported as a major clinical concern worldwide. The main aim of the present study was to determine the distribution of bla OXA, bla PER-1, bla VEB-1, and bla GES-1 genes among ESBL-producing P. aeruginosa isolated from two distinct provinces in Iran. METHODS: In this study, a total of 75 (27.5%) ESBL-producing isolates were identified from 273 P. aeruginosa isolates collected from patients in Qazvin and Tehran. Phenotypic detection of ESBLs and antimicrobial susceptibility testing were performed according to the Clinical and Laboratory Standards Institute guidelines. PCR and sequencing were employed to detect bla OXA-1, bla OXA, bla GES-1, bla PER-1, and bla VEB-1 genes. Isolate genetic relationships were evaluated by repetitive extragenic palindromic sequence-based PCR (REP-PCR). RESULTS: In total, 59 (78.7%) of the ESBL-producing isolates showed multidrug resistance. The highest rates of susceptibility were observed against colistin (75 isolates, 100%) and polymyxin B (75, 100%) followed by amikacin (44, 58.7%), and piperacillin-tazobactam (40, 53.3%). The bla OXA-1 (37.3%) gene was the most common of the genes investigated, followed by bla OXA-4 (32%), bla GES-1 (16%), and bla VEB-1 (13.3%). REP-PCR identified three different genotypes: types A (89.3%), B (6.7%), and C (4%). CONCLUSIONS: We found a significant presence of bla OXA-1, bla OXA-4, bla GES-1, and bla VEB-1 genes among P. aeruginosa isolates, highlighting the need for suitable infection control strategies to effectively treat patients and prevent the further distribution of these resistant organisms.
Descritores: Pseudomonas aeruginosa/genética
beta-Lactamases/genética
Farmacorresistência Bacteriana Múltipla/genética
Antibacterianos/farmacologia
-Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/enzimologia
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Proteínas de Escherichia coli/genética
Genes Bacterianos
Genótipo
Irã (Geográfico)
Limites: Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Id: biblio-957466
Autor: Sohail, Muhammad; Latif, Zakia.
Título: Molecular analysis, biofilm formation, and susceptibility of methicillin-resistant staphylococcus aureus strains causing community- and health care-associated infections in central venous catheters
Fonte: Rev. Soc. Bras. Med. Trop;51(5):603-609, Sept.-Oct. 2018. tab, graf.
Idioma: en.
Projeto: Higher Education Commission.
Resumo: Abstract INTRODUCTION: The behavior of methicillin-resistant Staphylococcus aureus (MRSA) isolated from central venous catheter-related infection was evaluated to determine its biofilm potential, antimicrobial resistance, and adhesion genes. METHODS: A total of 1,156 central venous catheters (CVC) were evaluated to screen for pathogens. Antimicrobial sensitivity, biofilm formation potential, and molecular analysis of MRSA were examined following standard guidelines. RESULTS: Of the 1,156 samples, 882 (76%) were colonized by bacteria or candida. Among the infected patients, 69% were male and 36% were female with median age of 32 years. Staphylococcus aureus infected 39% (344/882) of CVCs in patients. Of the 59% (208/344) of patients with MRSA, 57% had community acquired MRSA and 43% had hospital acquired MRSA. Linezolid and vancomycin killed 100% of MRSA; resistance levels to fusidic acid, doxycycline, clindamycin, azithromycin, amikacin, trimethoprim-sulfamethoxazole, gentamycin, tobramycin, and ofloxacin were 21%, 42%, 66%, 68%, 72%, 85%, 95%, 97%, and 98% respectively. Strong biofilm was produced by 23% of samples, moderate by 27%, and weak by 50% of MRSA. The presence of adhesion genes, sdrC and sdrD (90%), eno (87%), fnbA (80%), clfA and sdrE (67%), fnbB, sdrD (61%), and cna (51%), in most MRSA samples suggested that the adhesion genes are associated with biofilm synthesis. CONCLUSIONS: The superbug MRSA is a major cause of CVC-related infection. Antibiotic resistance to major classes of antibiotics and biofilm formation potential enhanced superbug MRSA virulence, leading to complicated infection. MRSA causes infection in hospitals, communities, and livestock.
Descritores: Infecções Estafilocócicas/microbiologia
Infecção Hospitalar/microbiologia
Infecções Comunitárias Adquiridas/microbiologia
Biofilmes/crescimento & desenvolvimento
Staphylococcus aureus Resistente à Meticilina/fisiologia
Infecções Relacionadas a Cateter/microbiologia
Antibacterianos/farmacologia
-Aderência Bacteriana/genética
Testes de Sensibilidade Microbiana
Biofilmes/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Genes Bacterianos/genética
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1042662
Autor: Silvagni, Marlene; Guillén, Rosa; Rodríguez, Fátima; Espínola, Carmen; Grau, Lorena; Velázquez, Gladys.
Título: Resistencia inducible a clindamicina en Staphylococcus aureus resistentes a meticilina aislados de pacientes pediátricos en Paraguay / Inducible resistance to clindamycin in methicillin-resistant Staphylococcus aureus isolated from Paraguayan children
Fonte: Rev. chil. infectol;36(4):455-460, ago. 2019. tab, graf.
Idioma: es.
Resumo: Resumen Introducción: El método de difusión de doble disco se presenta como una alternativa diagnóstica que permite identificar aislados de Staphylococcus aureus susceptibles a clindamicina, ante el aumento de resistencia a meticilina, reduciendo así la posibilidad de fallo en el tratamiento. Objetivo: Determinar la frecuencia de resistencia a clindamicina inducida por eritromicina en S. aureus resistentes a meticilina (SARM) aislados de niños paraguayos. Materiales y Métodos: Estudio observacional, descriptivo, de corte transversal. Se colectaron 145 aislados S. aureus que causaron infecciones de piel y tejidos blandos y osteo-articulares en pacientes pediátricos del Hospital Central del Instituto de Previsión Social en el período de diciembre-2012 a noviembre-2013. La resistencia a clindamicina se determinó por métodos automatizados y de difusión de doble disco. Se realizó reacción de polimerasa en cadena para genes ermA, ermB, ermC y msrA de aislados representativos. Resultados: La resistencia global a meticilina y clindamicina fue de 67 y 13%, respectivamente (11% atribuible al mecanismo de resistencia a clindamicina inducible). Los genes ermC y msrA fueron detectados individualmente en 25 y 17% de los aislados, respectivamente, mientras que un aislado presentó ambos genes en simultáneo. Discusión: La frecuencia de mecanismo de resistencia inducible a clindamicina señala la importancia de los métodos de difusión de doble disco en la práctica microbiológica, así como se encuentran en los límites de puntos de cortes considerados como aceptables para el uso de este antimicrobiano para infecciones cutáneas y osteo-articulares causadas por SARM.

Background: The double disc diffusion method is an alternative diagnostic that allows the identification of Staphylococcus aureus isolates apparently susceptible to clindamycin but that may develop resistance due to an induction phenomena, mainly asociated to the increase in resistance to methicillin, thus increasing the possibility of failure in the treatment. Aim: To determine the frequency of induced clindamycin resistance in methicillin-resistant S. aureus (MRSA) isolated from Paraguayan children. Materials and Methods: In this cross sectional study, we collected 145 S. aureus isolates that caused skin and soft tissue and osteoarticular infections in pediatric patients of the Central Hospital I.P.S. in the period from December-2012 to November-2013. Resistance to clindamycin was determined by automated methods and double disc diffusion. PCR was performed for ermA, ermB, ermC and msrA genes from representative isolates. Results: The global resistance to methicillin and clindamycin was 67 and 13%, respectively (11% attributable to the inducible mechanism). The ermC and msrA genes were detected individually in 25 and 17% of the isolates respectively while an isolate presented both genes simultaneously. Discussion: The frequency of inducible resistance to clindamycin indicates the importance of double disc diffusion methods in microbiological practice, as well as being within the cut off points considered acceptable for the use of this antibiotic for skin infections. and osteoarticular caused by MRSA.
Descritores: Infecções Estafilocócicas/microbiologia
Clindamicina/farmacologia
Farmacorresistência Bacteriana/genética
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Antibacterianos/farmacologia
-Paraguai
Reação em Cadeia da Polimerase
Estudos Transversais
Farmacorresistência Bacteriana/efeitos dos fármacos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Genes Bacterianos
Limites: Humanos
Recém-Nascido
Lactente
Pré-Escolar
Criança
Adolescente
Tipo de Publ: Estudo Observacional
Responsável: CL1.1 - Biblioteca Central


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Id: lil-780805
Autor: Berber, Ismet; Avsar, Cumhur; Yegin, Zeynep; Tekerci, Melike; Civek, Seyhan.
Título: Molecular epidemiology of Pseudomonas aeruginosa clinical isolates
Fonte: Braz. j. infect. dis;20(2):224-225, Mar.-Apr. 2016. graf.
Idioma: en.
Descritores: Pseudomonas aeruginosa/genética
Fatores de Virulência/genética
Antibacterianos/farmacologia
-Pseudomonas aeruginosa/efeitos dos fármacos
Turquia
DNA Bacteriano/análise
Epidemiologia Molecular
Genes Bacterianos/genética
Limites: Humanos
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: lil-789481
Autor: Pereira, Jussyêgles Niedja da Paz; Rabelo, Marcelle Aquino; Lima, Jailton Lobo da Costa; Neto, Armando Monteiro Bezerra; Lopes, Ana Catarina de Souza; Maciel, Maria Amélia Vieira.
Título: Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil
Fonte: Braz. j. infect. dis;20(3):276-281, May.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Introduction There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail. Objective To characterize the phenotypic (occurrence of cMLSB and iMLSB phenotypes) and molecular (occurrence of ermA and ermC genes) profiles of MLSB resistance of clinical isolates of susceptible and methicillin-resistant Staphylococcus aureus and CNS (coagulase-negative Staphylococcus) from patients of a university hospital, in Pernambuco. Methods The antimicrobial susceptibility of 103 isolates was determined by the disk diffusion technique in Mueller–Hinton agar followed by oxacillin screening. The iMLSB phenotype was detected by D test. Isolates with cMLSB and iMLSB phenotypes were subjected to polymerase chain reaction (PCR) for the detection of ermA and ermC genes. Results The cMLSB and iMLSB phenotypes were respectively identified in 39 (37.9%) and five (4.9%) isolates. The iMLSB phenotype was found only in four (10.8%) methicillin-susceptible S. aureus and one (4.5%) methicillin-resistant S. aureus. In the 44 isolates subjected to PCR, four (9.1%) only ermA gene was detected, a lower frequency when compared to only ermC 17 (38.6%) gene and to one (2.3%) isolate presenting both genes. Conclusion In the Staphylococcus spp. analyzed, the ermC gene was found more often than the ermA, although the iMLSB phenotype had been less frequent than the cMLSB. It was important to perform the D test for its detection to guide therapeutic approaches.
Descritores: Staphylococcus/efeitos dos fármacos
Staphylococcus/genética
Macrolídeos/farmacologia
Estreptogramina B/farmacologia
Farmacorresistência Bacteriana Múltipla/genética
Lincosamidas/farmacologia
-Fenótipo
Brasil
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Genes Bacterianos/genética
Hospitais Universitários
Limites: Humanos
Responsável: BR1.1 - BIREME



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