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Simón, Daniel
Lunge, Vagner Ricardo
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Id: lil-749868
Autor: Librelotto, Carina Sperotto; Gräf, Tiago; Simon, Daniel; Almeida, Sabrina Esteves Matos de; Lunge, Vagner Ricardo.
Título: HIV-1 epidemiology and circulating subtypes in the countryside of South Brazil
Fonte: Rev. Soc. Bras. Med. Trop;48(3):249-257, May-Jun/2015. tab, graf.
Idioma: en.
Resumo: INTRODUCTION: Human immunodeficiency virus type 1 (HIV-1) has spread worldwide, with several subtypes and circulating recombinant forms. Brazil has an incidence of 20.5 HIV-1/acquired immunodeficiency syndrome (AIDS) patients per 100,000 inhabitants; however, the Southernmost State of Rio Grande do Sul (RS) has more than twice the number of HIV-1-infected people (41.3/100,000 inhabitants) and a different pattern of subtype frequencies, as previously reported in studies conducted in the capital (Porto Alegre) and its metropolitan region. This study examined HIV-1/AIDS epidemiological and molecular aspects in the countryside of Rio Grande do Sul. METHODS: Socio-demographic, clinical and risk behavioral characteristics were obtained from HIV-1-positive adult patients using a structured questionnaire. HIV-1 subtypes were determined by nested-polymerase chain reaction (PCR) and sequencing of the pol and env genes. RESULTS: The study sample included 149 (55% women) patients with a mean age of 41.8 ± 11.9 years. Most (73.8%) patients had a low education level and reported heterosexual practices as the most (91.9%) probable transmission route. HIV-1 subtypes were detected in 26 patients: 18 (69.2%) infected with subtype C, six (23.1%) infected with subtype B and two (7.7%) infected with BC recombinant forms. CONCLUSIONS: These data highlight the increasing number of HIV-1 subtype C infections in the countryside of South Brazil. .
Descritores: Infecções por HIV/epidemiologia
HIV-1
-Brasil/epidemiologia
Estudos Transversais
Genótipo
Genes env/genética
Genes gag/genética
Infecções por HIV/virologia
HIV-1
Dados de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase
Fatores de Risco
Limites: Adulto
Feminino
Seres Humanos
Masculino
Responsável: BR1.1 - BIREME


  2 / 12 LILACS  
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Id: lil-614579
Autor: Cunha, L. K. H; Kashima, S; Amarante, M. F. C; Haddad, R; Rodrigues, E. S; Silva, K. L. T; Lima, T. A; Castro, D. B; Brito, F. C; Almeida, E. G; Covas, D. T; Malheiro, A.
Título: Distribution of human immunodeficiency virus type 1 subtypes in the state of Amazonas, Brazil, and subtype C identification
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;45(2):104-112, Feb. 2012. ilus, tab.
Idioma: en.
Resumo: Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic analysis was performed using the gag, pol, and env gene sequences. We collected samples from 31 blood donors who tested positive for HIV-1 in confirmatory experiments. The male:female ratio of blood donors was 3.4:1, and the mean age was 32.4 years (range: 19 to 61 years). Phylogenetic analysis showed that subtype B is the most prevalent among Northern Brazilian HIV-1-seropositive blood donors. One HIV-1 subtype C and one circulating recombinant form (CRF_BF) of HIV-1 were identified in the State of Amazonas. This is the first study showing the occurrence of a possible "homogenous" subtype C in this region of Brazil. This finding could contribute to a better characterization of the HIV-1 strains that circulate in the country.
Descritores: Doadores de Sangue
Infecções por HIV/virologia
HIV-1
-Sequência de Bases
Western Blotting
Brasil/epidemiologia
Ensaio de Imunoadsorção Enzimática
Genes env/genética
Genes gag/genética
Genes pol/genética
Infecções por HIV/epidemiologia
HIV-1
Dados de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase
Limites: Adulto
Feminino
Seres Humanos
Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 12 LILACS  
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Id: lil-577020
Autor: Taylor-Castillo, Lizeth; León-Bratti, María Paz; Solano-Chinchilla, Antonio; Herrera-Martínez, Gisela; Boza-Cordero, Ricardo; León, Bernal; Luftig, Ronald B; Visoná, Kirsten.
Título: Variability in HIV-1 partial genomic sequences in Costa Rican patients: analysis with different bioinformatics tools / Variabilidad de secuencias genómicas parciales del VIH-1 en pacientes costarricenses: análisis con diferentes herramientas bioinformáticas
Fonte: Rev. panam. salud pública = Pan am. j. public health;27(1):23-31, jan. 2010. ilus, graf, tab.
Idioma: en.
Resumo: OBJECTIVE: To estimate subtype and genomic variability in the HIV pol gene of Costa Rican patients by using different bioinformatics tools and to use this information to establish new policies to better manage these patients. METHODS: A total of 113 pol sequences available from Costa Rican patients under highly active antiretroviral therapy were analyzed by using the Genotyping, REGA, Stanford, and MEGA programs. The pol sequences came from 77 virologic failures (VF) and 36 basal samples (BS). Of the 77 VF, 22 also were sequenced in the env region. RESULTS: No major differences were found among the variables studied. However, there was a tendency for more variability in VF patients with a high baseline viral load. In the pol gene, 75 percent-83 percent of BS and 66 percent-75 percent of VF samples were pure B subtype by Genotyping and REGA, respectively. The other samples presented variations related mainly to circulating recombinant form CRF12 by genotyping or to CRF17 or -29 by phylogenetic analysis or a new possible BD recombinant with all programs. In the Stanford program, all variable samples showed a subtype B with high polymorphism. The variability in the env sequences was lower than that in the pol region. CONCLUSION: The B subtype is predominant in Costa Rican HIV-positive patients. There is high variability within sequences with potential recombination between B and F or D subtypes. The BD recombinant has not been previously reported. This high variability is likely the result of possible recombinant events, nonadherence to antiretroviral therapy, sexual intercourse without protection, and many sexual partners. Similar studies should be done in other countries in the Region, in particular in those places with extensive immigration, in order to decrease the possibility of virus variability as well as the cost of antiretroviral therapy.

OBJETIVOS: Determinar el subtipo y la variabilidad genómica del gen pol del VIH de pacientes costarricenses mediante diferentes herramientas bioinformáticas y el uso de esta información para establecer nuevas políticas para mejorar el diagnóstico y el tratamiento de estos pacientes. MÉTODOS: Se analizaron 113 secuencias del gen pol de pacientes costarricenses bajo tratamiento antirretrovírico de gran actividad mediante cuatro programas: Genotyping, REGA, Stanford y MEGA. Las secuencias pol analizadas provenían de 77 casos considerados fracasos virológicos (FV) y 36 muestras iniciales (MI). También se secuenció la región env de 22 de los 77 FV. RESULTADOS: No se encontraron diferencias importantes entre las variables estudiadas. No obstante, se observó una tendencia a una mayor variabilidad en los pacientes FV que tenían una elevada carga viral inicial. Con respecto al gen pol, 77-83 por ciento de las MI y 66-75 por ciento de las muestras de los FV eran del subtipo B puro según Genotyping y REGA, respectivamente. Las otras muestras presentaron variaciones relacionadas principalmente con la forma recombinante en circulación CRF-12 según Genotyping, con la CRF-17 o la CRF-29 según el análisis filogenético, o una nueva posible forma recombinante BD según todos los programas. Con el programa Stanford, todas las muestras variables reflejaron un subtipo B con elevado polimorfismo. La variabilidad de la secuencia env fue menor que la de la región pol. CONCLUSIONES: El subtipo B fue el predominante en los pacientes positivos al VIH en Costa Rica. Existe una alta variabilidad en las secuencias con una posible recombinación entre los subtipos B, y F o D. La forma recombinante BD no se había notificado antes. Esta elevada variabilidad parece ser el resultado de posibles eventos de recombinación, la falta de adhesión al tratamiento antirretrovírico, las relaciones sexuales sin protección y numerosas parejas sexuales. Se deben emprender estudios similares en otros países de la Región, en particular en los lugares con mucha inmigración, para reducir tanto la posibilidad de que el virus varíe como el costo del tratamiento antirretrovírico.
Descritores: Biologia Computacional/métodos
Variação Genética
Genoma Viral
Infecções por HIV/virologia
HIV-1
-Terapia Antirretroviral de Alta Atividade
Fármacos Anti-HIV/economia
Fármacos Anti-HIV/uso terapêutico
Costa Rica
Genes env
Genes pol
Infecções por HIV/tratamento farmacológico
Infecções por HIV/economia
Infecções por HIV/epidemiologia
HIV-1
Cooperação do Paciente
Filogenia
Recombinação Genética
Alinhamento de Sequência
Análise de Sequência de RNA
Software
Comportamento Sexual/estatística & dados numéricos
Carga Viral
Adulto Jovem
Limites: Adulto
Feminino
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 12 LILACS  
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Texto completo SciELO Chile
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Id: lil-567529
Autor: Valenzuela, Carlos Y; Flores, Sergio V; Cisternas, Javier.
Título: Fixations of the HIV-1 env gene refute neutralism: New evidence for pan-selective evolution
Fonte: Biol. Res;43(2):149-163, 2010. ilus.
Idioma: en.
Resumo: We examined 103 nucleotide sequences of the HIV-1 env gene, sampled from 35 countries and tested: I) the random (neutral) distribution of the number of nucleotide changes; II) the proportion of bases at molecular equilibrium; III) the neutral expected homogeneity of the distribution of new fxated bases; IV) the hypothesis of the neighbor infuence on the mutation rates in a site. The expected random number of fxations per site was estimated by Bose-Einstein statistics, and the expected frequencies of bases by matrices of mutation-fxation rates. The homogeneity of new fxations was analyzed using χ2 and trinomial tests for homogeneity. Fixations of the central base in trinucleotides were used to test the neighbor infuence on base substitutions. Neither the number of fxations nor the frequencies of bases ftted the expected neutral distribution. There was a highly signifcant heterogeneity in the distribution of new fxations, and several sites showed more transversions than transitions, showing that each nucleotide site has its own pattern of change. These three independent results make the neutral theory, the nearly neutral and the neighbor infuence hypotheses untenable and indicate that evolution of env is rather highly selective.
Descritores: Sequência de Bases/genética
Evolução Molecular
Genes env/genética
HIV-1
Seleção Genética/genética
-Mutação
Filogenia
Responsável: CL1.1 - Biblioteca Central


  5 / 12 LILACS  
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Id: lil-472833
Autor: Heslop, O. D; Smikle, M. F; Deer, D; Christian, N. A; Vickers, I. E; Harvey, K. M; Figueroa, J. P; Christie, C. D; Bain, B; Barton, E. N.
Título: Human immunodeficiency virus type-1 (HIV-1) subtypes in Jamaica
Fonte: West Indian med. j;54(5):279-282, Oct. 2005. ilus.
Idioma: en.
Resumo: The subtypes of 141 isolates of human immunodeficiency virus type-1 (HIV-1) from Jamaica were determined by a combination of env and gag heteroduplex mobility analysis (HMA) genotyping. The majority of HIV-1 isolates were subtype B (131/141, 93.0); one (0.8) isolate each of subtypes C, D and E was found and 7 (4.9) were indeterminate. These results and the failure of the sets of primers used to amplify some of the HIV-1 isolates provide strong evidence of genetic diversity of the HIV/AIDS epidemic in Jamaica. Surveillance of the circulating HIV-1 genetic subtypes is a pre-requisite for developing regional vaccine strategies and understanding the transmission patterns of the virus. This is the first study of its kind in Jamaica and the findings complement data from other Caribbean countries. This work supports the view of colleagues from the French and Spanish-speaking Caribbean that an epidemiological network supported by regional laboratories will help track this epidemic accurately with positive outcomes for the public.

Los subtipos de 141 aislados del virus tipo 1 de la inmunodeficiencia humno (VIH-1) en Jamaica, fueron determinados combinando la genotipificación por análisis de heterodúplex (HMA) en los genes env y gag. La mayor parte de los aislados HIV-1 fueron del subtipo B (131/141, 93.0%), se halló uno (0.8%) aislado para cada uno de los subtipos C, D y E, en tanto que 7 (4.9%) fueron indeterminados. Estos resultados y el fallo de los conjuntos de primers usados para amplificar algunos de los aislados de VIH-1, ofrecen fuerte evidencia de la diversidad epidémica del VIH/SIDA en Jamaica. La vigilancia de los subtipos genéticos de VIH-1 en circulación, constituye un pre-requisito, tanto para desarrollar estrategias de vacunas a nivel regional, como para entender los patrones de transmisión del virus. Este es el primer estudio de este tipo en Jamaica, y nuestros hallazgos complementan los datos obtenidos en otros países del Caribe. Coincidimos con nuestros colegas del Caribe francófono e hispano-parlante en cuanto a que una red epidemiológica apoyada por los laboratorios regionales, nos ayudaría a continuar rastreando esta epidemia con exactitud, y con resultados positivos para el público.
Descritores: Genes env
Genes gag
HIV-1
Infecções por HIV/epidemiologia
-Países em Desenvolvimento
DNA Viral/análise
HIV-1
Incidência
Infecções por HIV/diagnóstico
Jamaica/epidemiologia
Medição de Risco
Reação em Cadeia da Polimerase/métodos
Amostragem
Sensibilidade e Especificidade
Limites: Seres Humanos
Masculino
Feminino
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  6 / 12 LILACS  
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Morimoto, Helena Kaminami
Morimoto, Arilson Akira
Reiche, Edna Maria Vissoci
Matsuo, Tiemi
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Id: lil-460229
Autor: Morimoto, Helena Kaminami; Morimoto, Arilson Akira; Reiche, Edna Maria Vissoci; Ueda, Luiz Toshio; Matsuo, Tiemi; Reiche, Fernando Vissoci; Caterino-de-Araujo, Adele.
Título: Difficulties in the diagnosis of HTLV-2 infection in HIV/AIDS patients from Brazil: comparative performances of serologic and molecular assays, and detection of HTLV-2b subtype
Fonte: Rev. Inst. Med. Trop. Säo Paulo;49(4):225-230, Jul.-Aug. 2007. tab.
Idioma: en.
Resumo: The current diagnosis of human T-lymphotropic virus type-2 (HTLV-2) infection is based on the search of specific antibodies; nevertheless, several studies conducted in Brazil pointed deficiencies of the commercially available kits in detecting HTLV-2, mostly in HIV/AIDS patients. This study searched for the presence of HTLV-1 and -2 in 758 HIV/AIDS patients from Londrina, Paraná, Brazil. Serum samples were screened for HTLV-1/2 antibodies using two EIA kits (Vironostika and Murex), and confirmed by WB (HTLV Blot 2.4, Genelabs). The results obtained by EIA disclosed 49 (6.5 percent) reactive sera: 43 positive by both EIA kits, and six with discordant results. WB confirmed HTLV-1 infection in seven samples (0.9 percent) and HTLV-2 in 21 sera (2.8 percent). Negative and indeterminate results were detected in four (0.5 percent) and 16 (2.1 percent) sera, respectively. Blood from 47 out of 49 HTLV seroreactive patients were collected and analyzed for the presence of env, LTR and tax genomic segments of HTLVs by PCR. PCR confirmed six cases of HTLV-1 and 37 cases of HTLV-2 infection (14 out of 16 that were found to be WB indeterminate). Restriction analysis of the env PCR products of HTLV-2 disclosed 36 isolates of HTLV-2a/c subtype, and one of HTLV-2b subtype. These results emphasize the need of improving serologic tests for detecting truly HTLV-2 infected patients from Brazil, and confirm the presence of HTLV-2b subtype in the South of this country.

O diagnóstico de infecção por HTLV-2 se baseia na pesquisa de anticorpos específicos, entretanto, vários estudos conduzidos no Brasil têm apontado falhas nos kits sorológicos disponíveis no mercado em detectar HTLV-2, principalmente nos pacientes com HIV/aids. Este trabalho avaliou a presença de infecção por HTLV-1 e -2 em 758 pacientes HIV/aids de Londrina, Paraná, Brasil. Amostras de soro foram analisadas quanto à presença de anticorpos anti-HTLV-1/2 por dois kits de EIA (Vironostika e Murex) e confirmados por WB (HTLV Blot 2.4, Genelabs). Os resultados obtidos pelos testes sorológicos mostraram 49 (6,5 por cento) soros reagentes: 43 positivos para ambos os kits e seis com resultados discordantes. O WB confirmou infecção por HTLV-1 em sete soros (0,9 por cento) e HTLV-2 em 21 soros (2,8 por cento). Resultados negativos e indeterminados foram detectados, respectivamente, em quatro (0,5 por cento) e 16 (2,1 por cento) soros. Amostras de sangue de 47 dos 49 pacientes com sorologia reagente foram avaliadas quanto à presença de segmentos do genoma dos HTLVs (env, LTR e tax), usando a técnica de PCR. As PCRs confirmaram seis casos de infecção por HTLV-1 e 37 casos por HTLV-2 (14 dos 16 cuja sorologia resultou WB indeterminada). A subtipagem de HTLV-2 por análise de restrição enzimática de produtos da PCR env mostrou 36 isolados de subtipo HTLV-2a/c e um HTLV-2b. Esses resultados reforçam a necessidade de melhorar o diagnóstico de infecção por HTLV-2 no Brasil e confirmam a presença do subtipo HTLV-2b na região sul do país.
Descritores: Infecções por HIV/complicações
Infecções por HTLV-I/diagnóstico
Infecções por HTLV-II/diagnóstico
Vírus 1 Linfotrópico T Humano
HUMAN T-LYMPHOTROPIC VIRUS TEMEFOS
-Western Blotting
Estudos Transversais
DNA Viral/isolamento & purificação
Genes env/genética
Anticorpos Anti-HTLV-I/sangue
Infecções por HTLV-I/complicações
Anticorpos Anti-HTLV-II/sangue
Infecções por HTLV-II/complicações
Vírus 1 Linfotrópico T Humano/genética
Vírus 1 Linfotrópico T Humano/imunologia
/genética
HUMAN T-LYMPHOTROPIC VIRUS TEMEFOS/genética
/imunologia
HUMAN T-LYMPHOTROPIC VIRUS TEMEFOS/imunologia
Técnicas Imunoenzimáticas
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Sensibilidade e Especificidade
Limites: Seres Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  7 / 12 LILACS  
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Id: lil-440576
Autor: Cabral, Valéria P; Cunha, Carla B; Magalhaes, Eneida F. L; Pinto-Neto, Lauro F; Couto-Fernandez, José Carlos; Dietze, Reynaldo; Morgado, Mariza G; Ribeiro-Rodrigues, Rodrigo.
Título: Human immunodeficiency virus type- 1 subtypes of infected patients in Espírito Santo, Brazil
Fonte: Mem. Inst. Oswaldo Cruz;101(8):881-885, Dec. 2006. tab, ilus.
Idioma: en.
Resumo: Genetic variability of human immunodeficiency virus type - 1(HIV-1) is a potential threat for both diagnosis and treatment of HIV/AIDS, as well as the development of effective vaccines. Up to now, HIV subtypes circulating among HIV-positive patients in the state of Espírito Santo were not known. In the present study, blood samples from 100 therapy-naïve HIV-1 infected patients were collected and the HIV subtype was determined through the Heteroduplex Mobility Assay (HMA). Ninety-seven out of 100 studied samples were subtyped by HMA, 73 samples (75.2 percent) were from subtype B, 9 (9.3 percent) from subtype F, 3 (3.1 percent) from subtype C, 6 (6.2 percent) Benv/Fgag, and another 6 (6.2 percent) Fenv/Bgag, what suggests that recombinant viruses were present in the studied samples. Twenty-eight percent of the subtype B samples were represented by the Brazilian B" subtype, which were identified by RFLP with Fok I. Data presented here demonstrate that the epidemiological characteristics of the HIV epidemic in the state of Espírito Santo are similar to those from the other Southeastern states and helped to better understand the genetic polymorphism of HIV in Brazil.
Descritores: Variação Genética
Genes env/genética
Genes gag/genética
Infecções por HIV/virologia
HIV-1
-Brasil
Análise Heteroduplex
HIV-1
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Responsável: BR1.1 - BIREME


  8 / 12 LILACS  
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Id: lil-344283
Autor: Balcázar, Norman; Sánchez, Gloria I; Garcia-Vallejo, Felipe.
Título: Sequence and phylogenetic analysis of human T cell lymphotropic virus type 1 from Tumaco, Colombia
Fonte: Mem. Inst. Oswaldo Cruz;98(5):641-648, July 2003. ilus, tab, graf.
Idioma: en.
Resumo: Human T cell lymphotropic virus type 1 (HTLV-1) is a retrovirus that causes leukemia and the neurological disorder HTLV-1 associated myelopathy or tropical spastic paraparesis (HAM/TSP). Infection with this virus - although it is distributed worldwide - is limited to certain endemic areas of the world. Despite its specific distribution and slow mutation rate, molecular epidemiology on this virus has been useful to follow the movements of human populations and routes of virus spread to different continents. In the present study, we analyzed the genetic variability of a region of the env gene of isolates obtained from individuals of African origin that live on the Pacific coast of Colombia. Sequencing and comparison of the fragment with the same fragment from different HTLV-1 isolates showed a variability ranging from 0.8 percent to 1.2 percent. Phylogenetic studies permit us to include these isolates in the transcontinental subgroup A in which samples isolated from Brazil and Chile are also found. Further analyses will be necessary to determine if these isolates were recently introduced into the American continent or if they rather correspond to isolates introduced during the Paleolithic period
Descritores: DNA Viral
Genes env
Vírus 1 Linfotrópico T Humano
Filogenia
-Sequência de Aminoácidos
Colômbia
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  9 / 12 LILACS  
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Morgado, Mariza Gonçalves
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Id: lil-344235
Autor: Gadelha, Sandra Rocha; Shindo, Nice; Cruz, José Napoleäo Monte; Morgado, Mariza Gonçalves; Galvão-Castro, Bernardo.
Título: Molecular epidemiology of human immunodeficiency virus-1 in the state of Ceará, Northeast, Brazil
Fonte: Mem. Inst. Oswaldo Cruz;98(4):461-463, June 2003. ilus, tab.
Idioma: en.
Resumo: We analyzed, by env and gag heteroduplex mobility assay, 149 human immunodeficiency virus (HIV-1) positive samples collected in Ceará during the year 2000. The prevalence of subtype B was 81.2 percent and the prevalence of subtype F and B/F recombinants were both 2.7 percent. Eight (5.4 percent) and 12 (8 percent) out of 149 samples showed indeterminate results in the env and gag analysis respectively. By FokI restriction fragment length polymorphism, 34 percent of the subtype B samples were identified as the typical Brazilian subtype B.In the present study, we identified HIV-1 subtype F and B/F in Ceará for the first time. Our results contribute to the understanding of HIV in Brazil, and may prove useful for the development of vaccine candidates
Descritores: DNA Viral
Genes env
Genes gag
Infecções por HIV
HIV-1
-Brasil
Variação Genética
Análise Heteroduplex
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Prevalência
Limites: Criança
Adolescente
Adulto
Seres Humanos
Masculino
Feminino
Meia-Idade
Responsável: BR1.1 - BIREME


  10 / 12 LILACS  
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Id: lil-317323
Autor: Melana, Stella M; Picconi, Maria Alejandra; Rossi, Carlos; Mural, Juan; Alonio, Lidia Virginia; Teyssie, Angelica; Holland, James F; Pogo, Beatriz G. T.
Título: Deteccion de secuencias homologas al gen env del virus del Tumor Mamario Murino (MMTV) en cancer de mama de pacientes argentinas / Detection of murine mammary tumor virus (MMTV) env gene-like sequences in breast cancer from Argentine patients
Fonte: Medicina (B.Aires);62(4):327-323, 2002. ilus, tab.
Idioma: es.
Resumo: In the last years research on the possible viral etiology of human breast cancer has been revised. Previous studies have demonstrated the presence of a Mouse Mammary Tumor Virus (MMTV) env gene-like sequence in about 38% of breast cancers from American and Italian women; these sequences are generally absent in other tumors and in normal mammary tissue. In the present study we have analyzed the presence of a 250-bp sequence of the MMTV env gene in breast cancer biopsies from Argentine patients. The retroviral fragment was present in 31% (23/74) of the tumors, only in one normal mammary tissue and in none of the fibroadenomas analYzed. Peripheral blood mononuclear cells (PBMC) from 46 cancer patients were also analyzed; the sequence was found in 17% (2/12) of the PBMC from env positive tumor patients and in 3% (1/34) of the env negatives. The results from Argentine samples are similar to those from USA and Italy, where the breast cancer incidence is alike. These findings support the hypothesis of a viral agent involved in the genesis of this neoplasia and encourage the continuation of these studies
Descritores: Neoplasias da Mama
Genes env
Vírus do Tumor Mamário do Camundongo
Infecções por Retroviridae
Infecções Tumorais por Vírus
-Argentina
Sequência de Bases
Homologia de Sequência
Limites: Seres Humanos
Animais
Feminino
Camundongos
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde