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Id: biblio-1022130
Autor: Zhou, Shushan; Jiang, Li; Guan, Shuangxue; Gao, Yongxia; Gao, Qinghua; Wang, Guangdong; Duan, Ke.
Título: Expression profiles of five FT-like genes and functional analysis of PhFT-1 in a Phalaenopsis hybrid
Fonte: Electron. j. biotechnol;31:75-83, Jan. 2018. tab, ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Background: Phalaenopsis is an important ornamental flowering plant that belongs to the Orchidaceae family and is cultivated worldwide. Phalaenopsis has a long juvenile phase; therefore, it is important to understand the genetic elements regulating the transition from vegetative phase to reproductive phase. In this study, FLOWERING LOCUS T (FT) homologs in Phalaenopsis were cloned, and their effects on flowering were analyzed. Results: A total of five FT-like genes were identified in Phalaenopsis. Phylogenetic and expression analyses of these five FT-like genes indicated that some of these genes might participate in the regulation of flowering. A novel FT-like gene, PhFT-1, distantly related to previously reported FT genes in Arabidopsis and other dicot crops, was also found to be a positive regulator of flowering as heterologous expression of PhFT-1 in Arabidopsis causes an early flowering phenotype. Conclusions: Five FT homologous genes from Phalaenopsis orchid were identified, and PhFT-1 positively regulates flowering.
Descritores: Proteínas de Plantas/genética
Arabidopsis
Orchidaceae/genética
Flores/genética
-Reação em Cadeia da Polimerase/métodos
Clonagem Molecular
Genes de Plantas/genética
Biologia Computacional
Orchidaceae/crescimento & desenvolvimento
Flores/crescimento & desenvolvimento
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1017082
Autor: Mei, Lanju; Dong, Na; Li, Fosheng; Li, Na; Yao, Min; Chen, Fang; Tang, Lin.
Título: Transcriptome analysis of female and male flower buds of Idesia polycarpa Maxim. var. vestita Diels
Fonte: Electron. j. biotechnol;29:39-46, sept. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Science and Technology support projects of Sichuan Province.
Resumo: Background: Idesia polycarpa Maxim. var. vestita Diels, a dioecious plant, is widely used for biodiesel due to the high oil content of its fruits. However, it is hard to distinguish its sex in the seedling stage, which makes breeding and production problematic as only the female tree can produce fruits, and the mechanisms underlying sex determination and differentiation remain unknown due to the lack of available genomic and transcriptomic information. To begin addressing this issue, we performed the transcriptome analysis of its female and male flower. Results: 28,668,977 and 22,227,992 clean reads were obtained from the female and male cDNA libraries, respectively. After quality checks and de novo assembly, a total of 84,213 unigenes with an average length of 1179 bp were generated and 65,972 unigenes (78.34%) could be matched in at least one of the NR, NT, Swiss-Prot, COG, KEGG and GO databases. Functional annotation of the unigenes uncovered diverse biological functions and processes, including reproduction and developmental process, which may play roles in sex determination and differentiation. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed many unigenes annotated as metabolic pathways, biosynthesis of secondary metabolites pathways, plant­ pathogen interaction, and plant hormone signal transduction. Moreover, 29,953 simple sequence repeats were identified using the microsatellite software. Conclusion: This work provides the first detailed transcriptome analysis of female and male flower of I. polycarpa and lays foundations for future studies on the molecular mechanisms underlying flower bud development of I. polycarpa.
Descritores: Reprodução/genética
Salicaceae/genética
Transcriptoma
-Análise de Sequência de RNA
Genes de Plantas
Repetições de Microssatélites
Salicaceae/crescimento & desenvolvimento
Bases de Dados Genéticas
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1015856
Autor: He, Meijing; Cui, Shunli; Yang, Xinlei; Mu, Guojun; Chen, Huanying; Liu, Lifeng.
Título: Selection of suitable reference genes for abiotic stress-responsive gene expression studies in peanut by real-time quantitative PCR
Fonte: Electron. j. biotechnol;28:76-86, July. 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . 948 Project; . Special Fund for Modern Agro-industry Technology Research System of China; . Key Technologies Research and Development Program of Hebei Province.
Resumo: Background: Because of its strong specificity and high accuracy, real-time quantitative PCR (RT-qPCR) has been a widely used method to study the expression of genes responsive to stress. It is crucial to have a suitable set of reference genes to normalize target gene expression in peanut under different conditions using RT-qPCR. In this study, 11 candidate reference genes were selected and examined under abiotic stresses (drought, salt, heavy metal, and low temperature) and hormone (SA and ABA) conditions as well as across different organ types. Three statistical algorithms (geNorm, NormFinder and BestKeeper) were used to evaluate the expression stabilities of reference genes, and the comprehensive rankings of gene stability were generated. Results: The results indicated that ELF1B and YLS8 were the most stable reference genes under PEG-simulated drought treatment. For high-salt treatment using NaCl, YLS8 and GAPDH were the most stable genes. Under CdCl2 treatment, UBI1 and YLS8 were suitable as stable reference genes. UBI1, ADH3, and ACTIN11 were sufficient for gene expression normalization in low-temperature experiment. All the 11 candidate reference genes showed relatively high stability under hormone treatments. For organs subset, UBI1, GAPDH, and ELF1B showed the maximum stability. UBI1 and ADH3 were the top two genes that could be used reliably in all the stress conditions assessed. Furthermore, the necessity of the reference genes screened was further confirmed by the expression pattern of AnnAhs. Conclusions: The results perfect the selection of stable reference genes for future gene expression studies in peanut and provide a list of reference genes that may be used in the future.
Descritores: Arachis/genética
Genes de Plantas
Reação em Cadeia da Polimerase em Tempo Real/métodos
-Reguladores de Crescimento de Planta
Padrões de Referência
Seleção Genética
Estresse Fisiológico
Amplificação de Genes
Temperatura Baixa
Perfilação da Expressão Gênica
Secas
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Saúde Pública
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Id: lil-761805
Autor: Irrazábal, Gabriela.
Título: Religión y salud: la intervención pública de agentes religiosos católicos formados en bioética en el debate parlamentario sobre la muerte digna en la Argentina / Religion and health: the public intervention of Catholic religious agents trained in bioethics in the parliamentary debate on death with dignity in Argentina
Fonte: Salud colect;11(3):331-349, jul.-sep. 2015.
Idioma: es.
Resumo: Desde una perspectiva sociológica, este trabajo aborda una de las aristas de la intervención pública de ciertos sectores del catolicismo en la elaboración y sanción de leyes de salud. En particular se hace foco en el debate en comisiones parlamentarias sobre la llamada ley de "muerte digna" (Ley 26742) en el cual se convocó a un grupo de expertos en bioética para asesorar a los senadores sobre los alcances y límites de la ley. La mayoría de los expertos invitados pregonan la perspectiva de la bioética personalista, un desarrollo teológico de la bioética del catolicismo contemporáneo. En el debate no participaron representantes de otros credos consolidando la ampliamente estudiada imbricación entre el catolicismo y lo político en Argentina.

This paper discusses from a sociological perspective one of Catholicism's fronts of public intervention in the development and enactment of health legislation. In particular we analyze the debate in parliamentary committees on the so-called "death with dignity" law (No. 26742), for which a group of bioethics experts was convened to counsel senators regarding the scope and limits of the law. The majority of the invited experts advocated a personalist bioethics perspective, which is a theological bioethics development of contemporary Catholicism. In the debate no representatives of other faiths were present, reinforcing the widely studied overlap between Catholicism and politics in Argentina.
Descritores: Mesembryanthemum/fisiologia
RNA de Plantas/genética
Salinidade
Análise de Sequência de RNA
-Arabidopsis/efeitos dos fármacos
Arabidopsis/crescimento & desenvolvimento
Genes de Plantas
Mesembryanthemum/genética
Raízes de Plantas/crescimento & desenvolvimento
Cloreto de Sódio/farmacologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-757058
Autor: Musso, Carlos G; Enz, Paula A.
Título: El arte como instrumento para comprender la diferencia entre información, conocimiento y saber / Art as an instrument to understand the difference between information, knowledge and knowing
Fonte: Arch. argent. pediatr;113(5):388-389, oct. 2015.
Idioma: es.
Descritores: Cruzamento
Embaralhamento de DNA
Lilium/genética
Transformação Genética
-Clonagem Molecular
Flores/genética
Regulação da Expressão Gênica de Plantas
Redes Reguladoras de Genes
Genes de Plantas
Regeneração
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Texto completo SciELO Costa Rica
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Id: lil-753716
Autor: Minghui, Fu; Lihua, Jiang; Yuanmei, Li; Guohua, Yan; Lijun, Zheng; Jinping, Peng.
Título: Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) / Identificación de fragmentos de genes relacionados con la deficiencia de nitrógeno en Eichhornia crassipes (Pontederiaceae)
Fonte: Rev. biol. trop;62(4):1637-1648, oct.-dic. 2014. graf, tab.
Idioma: en.
Resumo: Eichhornia crassipes is an aquatic plant native to the Amazon River Basin. It has become a serious weed in freshwater habitats in rivers, lakes and reservoirs both in tropical and warm temperate areas worldwide. Some research has stated that it can be used for water phytoremediation, due to its strong assimilation of nitro- gen and phosphorus, and the accumulation of heavy metals, and its growth and spread may play an important role in environmental ecology. In order to explore the molecular mechanism of E. crassipes to responses to nitrogen deficiency, we constructed forward and reversed subtracted cDNA libraries for E. crassipes roots under nitrogen deficient condition using a suppressive subtractive hybridization (SSH) method. The forward subtraction included 2 100 clones, and the reversed included 2 650 clones. One thousand clones were randomly selected from each library for sequencing. About 737 (527 unigenes) clones from the forward library and 757 (483 unigenes) clones from the reversed library were informative. Sequence BlastX analysis showed that there were more transporters and adenosylhomocysteinase-like proteins in E. crassipes cultured in nitrogen deficient medium; while, those cultured in nitrogen replete medium had more proteins such as UBR4-like e3 ubiquitin- protein ligase and fasciclin-like arabinogalactan protein 8-like, as well as more cytoskeletal proteins, including actin and tubulin. Cluster of Orthologous Group (COG) analysis also demonstrated that in the forward library, the most ESTs were involved in coenzyme transportation and metabolism. In the reversed library, cytoskeletal ESTs were the most abundant. Gene Ontology (GO) analysis categories demonstrated that unigenes involved in binding, cellular process and electron carrier were the most differentially expressed unigenes between the forward and reversed libraries. All these results suggest that E. crassipes can respond to different nitrogen status by efficiently regulating and controlling some transporter gene expressions, certain metabolism processes, specific signal transduction pathways and cytoskeletal construction.

Se ha convertido en una maleza importante en hábitats de agua dulce en ríos, lagos y embalses, tanto en zonas tropicales como templadas de todo el mundo. Algunas investigaciones han indicado que se puede utilizar para la fitorremediación de agua, debido a su fuerte asimilación de nitrógeno y fósforo, y la acumulación de metales pesados, su crecimiento y propagación puede desempeñar un papel importante en la ecología ambiental. Con el fin de explorar el mecanismo molecular de respuesta a la deficiencia de nitrógeno en E. crassipes, se construyeron bibliotecas de cDNA mediante síntesis adelantada y retrasada para raíces de E. crassipes en condiciones de deficiencia de nitrógeno mediante el método de hibridación supresiva sustractiva (SSH). Para este estudio se utilizaron 2 100 clones de síntesis adelantada y 2 650 de síntesis retrasada. De la biblioteca se escogieron al azar mil clones, 737 (527 unigenes) de síntesis adelanta- da y 757 (483 unigenes) de síntesis retrasada que fueron informativos. El análisis BLASTX mostró que había más transportadores y proteínas adenosilhomocisteinasa en E. crassipes cultivadas en un medio deficiente de nitrógeno; mientras que las cultivadas en un medio repleto de nitróge- no tenían más proteínas como UBR4 e3 ubiquitina-proteína ligasa y la proteína arabinogalactano 8 tipo fasciclina, así como otras proteínas del citoesqueleto, incluyendo la actina y la tubulina. Clúster del Grupo Ortológico (COG) también demostró que en la biblioteca de síntesis adelan- tada, la mayoría de los marcadores de secuencia expresada (ESTs) estaban involucrados en el transporte de coenzimas y el metabolismo.
Descritores: Recuperação e Remediação Ambiental
Etiquetas de Sequências Expressas
Eichhornia/genética
Genes de Plantas
Nitrogênio/metabolismo
Fósforo/metabolismo
-Eichhornia/classificação
Eichhornia/metabolismo
Biblioteca Gênica
Nitrogênio/deficiência
Reação em Cadeia da Polimerase
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Texto completo SciELO Brasil
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Id: lil-695247
Autor: Mendes, A.D.R.; Martins, E.R.; Figueiredo, L.S.
Título: Estudo do sistema de reprodução da fava-d'anta (Dimorphandra mollis Benth. ) / Determination of the reproductive system of fava-d'anta (Dimorphandra mollis Benth. )
Fonte: Rev. bras. plantas med;15(4):605-608, 2013. tab.
Idioma: pt.
Resumo: Entre as plantas nativas de uso medicinal do Cerrado brasileiro encontra-se a fava-d'anta (Dimorphandra mollis Benth) com alto potencial econômico por possuir inúmeras potencialidades medicinais e fitoquímicas. A indústria extrai dos frutos os princípios ativos rutina, quercetina, e ramnose, dentre outros, usados na fabricação de medicamentos e cosméticos, principalmente no exterior. O conhecimento do sistema reprodutivo da espécie é fundamental para sua conservação e manejo. O presente trabalho objetivou determinar as características morfométricas das flores e caracterizar o sistema reprodutivo da D. mollis em área de Cerrado inalterada antropomorficamente, no município de Olhos D'água - MG. Para as características morfométricas, diâmetro da flor, comprimento da flor, do ovário, e da antera, foram utilizadas cinco flores em pré-antese. Para a determinação do sistema reprodutivo utilizou-se a razão pólen:óvulo (P:O), em 40 flores. As flores da D. mollis apresentaram comprimento da flor de 3,00 mm, diâmetro da flor de 2,00 mm, comprimento do óvulo de 2,60 mm, comprimento da antera de 1,57 mm, e o número de óvulos e de anteras foram 20 e 5,8, respectivamente. A razão P:O foi 765,030, sendo que esse índice não é afetado pelas características morfométricas. O sistema reprodutivo da D. mollis foi classificado como alógama facultativa.

Among the native medicinal plants of the Brazilian Cerrado, we can find the fava-d'anta (Dimorphandra mollis Benth) with a high economic potencial due to its numerous possivbilities for medicinal and phytochemical use. The industry extracts form the fruits the active ingredients rutin, quercetin and rhamnose, among others, used to manufacture medications and cosmetic products, especially abroad. Knowing the reproductive system of the species is important for its conservation and management. This study aimed to determine the morphometric characteristics of flowers and characterize the reproductive system of D. mollis in a Cerrado area that was anthropomorphically unchanged, in the municipality of Olhos D'água - MG. For the morphometric characteristics, flower diameter, length of the flower, of the ovary and the anther, five pre-anthesis flowers were used. To determine the reproductive system, the pollen:ovule (P:O) ratio was used in 40 flowers. The D. mollis flowers presented a flower length of 3.00 mm, flower diameter of 2.00 mm, ovule length of 2.60 mm, anthers length of 1.57 mm, and the number of anthers and ovules were 20 and 5.8, respectively. The P:O ratio was 765,030, and this index is not affected by the morphometric characteristics. The reproductive system of D. mollis was classified as facultative allogamous.
Descritores: Fabaceae/classificação
Plantas Medicinais/genética
Reprodução/genética
-Genes de Plantas
Pradaria
Responsável: BR1.1 - BIREME


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Id: lil-671876
Autor: Valdés-Infante Herrero, Juliette; Rodríguez Medina, Narciso Nerdo; González, Lien; Velázquez Palenzuela, Josefa Bárbara; Rivero Rodríguez, Domingo; Sourd Martínez, Darío Gaspar; Martínez González, Felina; Rodríguez Rodríguez, Julio Alberto.
Título: La biotecnología como herramienta para la propagación, conservación y el mejoramiento genético del guayabo / Biotechnology as a tool for propagation, con-servation and genetic breeding in guava
Fonte: Rev. colomb. biotecnol;14(2):7-19, dic. 2012. ilus, tab.
Idioma: es.
Resumo: Las técnicas biotecnológicas contribuyen positiva y significativamente en los programas de propagación, conservación y mejoramiento de las especies vegetales. Dentro de éstas, el cultivo de tejidos, el desarrollo de mapas de ligamientos genéticos y de QTLs y la detección de genes de interés han demostrado ser de gran utilidad para los mencionados propósitos. En este sentido, se estandarizó una técnica para la multiplicación in vitro de la forma silvestre de guayabo en tres fases de cultivo: establecimiento, multiplicación de propágulos y enraizamiento. La misma constituye una vía de utilidad para la propagación, la conservación de germoplasma y el mejoramiento genético en la especie. Además, se estandarizó un método de conservación a corto-mediano plazo. Por otra parte, se construyó un mapa de ligamiento genético para la especie empleando marcadores AFLP y SSR. Los 11 grupos del mapa de ligamiento genético y los 50 QTLs relacionados con caracteres vegetativos y de calidad interna y externa del fruto, constituyen el punto de partida para el clonaje de genes de interés agrícola y la implementación futura de la selección asistida por marcadores en el guayabo. De igual forma, las 176 secuencias candidatas a genes de resistencia (RGL) y del desarrollo de la planta (MADS-box y HOMEO-box) detectadas pueden ser de gran utilidad en la saturación del mapa de ligamiento referido, el estudio de la variabilidad presente en el cultivo, así como en la solución de problemas relacionados con el rendimiento, la producción y la resistencia a estrés biótico y abiótico

Biotechnologies contribute positively and signifi¬cantly in the propagation, conservation and breeding programs of many plant species. From them, tissue culture, linkage maps and QTLs detection for interesting genes have been proved to be of great utility for these purposes. In this sense, a technique for in vitro multiplication of wild guava was standardized in three culture phases: establishment, multiplication and rooting. This technique constituted a useful way for propagation, germplasm conservation and genetic breeding in the specie. A method for short-medium term conservation was also standardized. On the other hand, a genetic linkage map was constructed for the specie using AFLP and SSR markers. The 11 groups of the genetic linkage map and the 50 QTLs related with vegetative and internal/external fruit characters constitute the starting point for genes cloning of agricultural interest and the future imple¬mentation of markers assisted selection in guava. Also, the 176 candidate sequences for resistance-gene-like (RGL) and plant development (MADS-box and HOMEO-box) genes detected can be of great utility in linkage map saturation, variability studies in this crop, as well as in the solution of problems rela¬ted with yielding and resistance to biotic and abiotic stresses.
Descritores: Genes
Psidium
-Genes Essenciais
Genes Modificadores
Genes de Plantas
Plantas
Responsável: CO326 - Departamento de Biología


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Id: lil-640984
Autor: Buso, G. S. C; Paiva, M. R; Torres, A. C; Resende, F. V; Ferreira, M. A; Buso, J. A; Dusi, A. N.
Título: Genetic diversity studies of Brazilian garlic cultivars and quality control of garlic-clover production
Fonte: Genet. mol. res. (Online);7(2):534-541, 2008. tab, ilus.
Idioma: en.
Resumo: The garlic cultivars grown in Brazil evolved from somatic mutations and clone selection by breeding programs and by the introduction of germplasm from other countries. Morphological characters have been used to differentiate these cultivars. Two hundred and six random amplified polymorphic DNA markers were utilized for a diversity analysis of the 17 most planted garlic cultivars in Brazil. Bootstrap analysis showed that the number of markers was efficient and sufficient to obtain a coefficient of variation of 10%. Similarity varied between 16 and 98% and cluster analysis showed that, in general, genetic similarities correlate with morphological characters of the cultivars and production cycle variation. High bootstrap values at most of the nodes supported the dendrogram stability. The grouping of most varieties agreed well with previous reports based on morphological characters. As a vegetative-propagated species, viral diseases are a key problem regarding production and quality of the bulbs, causing gradual loss of yield and decrease in storage capacity. To improve the health quality of garlic seed, a virus-free stock of garlic cloves of the Amarante cultivar was obtained. The ability to distinguish garlic cultivars to detect varietal mixing after in vitro multiplication is extremely important, since correct identification is not possible until bulbs are produced. Random amplified polymorphic DNA markers were also used to differentiate cultivars while they are in vitro and not amenable to morphological discrimination. No difference was identified between the fingerprints of the virus-free or of the infected bulks of Amarante, showing that there was no clove mixing in the handling of material in the clonal multiplication phase.
Descritores: Alho/citologia
Alho/genética
Variação Genética
-Cultivos Agrícolas
Alho/classificação
Brasil
Eficiência
Genes de Plantas
Marcadores Genéticos/fisiologia
Fotoperíodo
Filogenia
Controle de Qualidade
Técnica de Amplificação ao Acaso de DNA Polimórfico
Responsável: BR26.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: lil-640523
Autor: Shan, Shi-hua; Zhang, Ting-ting; Li, Chun-juan; Yang, Chen; Yan, Cai-xia; Wan, Shu-bo.
Título: Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L. )
Fonte: Electron. j. biotechnol;14(6):6-6, Nov. 2011. ilus, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Modern Agro-industry Technology Research System; . National Key Project of Scientific and Technical Supporting Programs of China.
Resumo: Background: Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66 percent). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties.
Descritores: Arachis/genética
Doenças das Plantas/genética
Genes de Plantas
Imunidade Inata/genética
-DNA Complementar/genética
Clonagem Molecular
Biologia Computacional
Genoma de Planta
Reação em Cadeia da Polimerase/métodos
Responsável: CL1.1 - Biblioteca Central



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