Base de dados : LILACS
Pesquisa : G05.360.340.024.340.393 [Categoria DeCS]
Referências encontradas : 51 [refinar]
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Id: biblio-886710
Autor: OLIVEIRA, FREDERICO I C DE; FIEGE, LEONARDO B C; CELIN, ELAINE F; INNECCO, RENATO; NUNES, GLAUBER H S; ARAGÃO, FERNANDO A S DE.
Título: Screening of melon genotypes for resistance to vegetable leafminer and your phenotypic correlations with colorimetry
Fonte: An. acad. bras. ciênc;89(2):1155-1166, Apr.-June 2017. tab.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: ABSTRACT Melon is one of the most important vegetable crops in the world. With short cycle in a system of phased planting, phytosanitary control is compromised, and a great volume of agricultural chemicals is used to control vegetable leafminer. Genetic control is an ideal alternative to avoid the damage caused by this insect. Thus, the aim of this study was to evaluate Cucumis accessions in regard to resistance to leafminer and correlate the variables analyzed. Fifty-four accessions and four commercial hybrids of melon were tested. The study was divided into two experiments: with and with no choice. The following characteristics were evaluated: with choice, in field - subjective score based on the infestation and the number of mines per leaf; and with no choice, in cage - number of mines per leaf, chlorophyll content, and leaf colorimetry. The results showed variability among the accessions and some genotypes showed favorable results for resistance in both experiments. There was correlation between the two variables in the experiment in the field. The accessions CNPH 11-282, CNPH 06-1047, and CNPH 11-1077 are the most recommended for future breeding programs with aim on introgression of resistance to vegetable leafminer in melon.
Descritores: Fenótipo
Controle Biológico de Vetores/métodos
Folhas de Planta/genética
Cucumis melo/genética
Dípteros
Genótipo
-Doenças das Plantas/prevenção & controle
Valores de Referência
Variação Genética
Clorofila/análise
Genes de Plantas
Colorimetria/métodos
Estatísticas não Paramétricas
Folhas de Planta/química
Larva
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-1087162
Autor: Jiménez-Guillen, Doribet; Pérez-Pascual, Daniel; Souza-Perera, Ramón; Zúñiga Aguilar, José Juan.
Título: Cloning and molecular characterization of a putative habanero pepper SERK1 cDNA expressed during somatic and zygotic embryogenesis
Fonte: Electron. j. biotechnol;41:48-55, sept. 2019. tab, ilus, graf.
Idioma: en.
Projeto: Consejo Nacional de Ciencia y Tecnología-FORDECyT; . Comisión Intersecretarial de Bioseguridad de los Organismos Genéticamente Modificado.
Resumo: Background: Plant gene homologs that control cell differentiation can be used as biotechnological tools to study the in vitro cell proliferation competence of tissue culture-recalcitrant species such as peppers. It has been demonstrated that SERK1 homologs enhance embryogenic competence when overexpressed in transformed tissues; therefore, cloning of a pepper SERK1 homolog was performed to further evaluate its biotechnological potential. Results: A Capsicum chinense SERK full-length cDNA (CchSERK1) was cloned and characterized at the molecular level. Its deduced amino acid sequence exhibits high identity with sequences annotated as SERK1 and predicted-SERK2 homologs in the genomes of the Capsicum annuum CM-334 and Zunla-1 varieties, respectively, and with SERK1 homologs from members of the Solanaceae family. Transcription of CchSERK1 in plant tissues, measured by quantitative RT-PCR, was higher in stems, flowers, and roots but lower in leaves and floral primordia. During seed development, CchSERK1 was transcribed in all zygotic stages, with higher expression at 14 days post anthesis. During somatic embryogenesis, CchSERK1 was transcribed at all differentiation stages, with a high increment in the heart stage and lower levels at the torpedo/cotyledonal stages. Conclusion: DNA sequence alignments and gene expression patterns suggest that CchSERK1 is the C. chinense SERK1 homolog. Significant levels of CchSERK1 transcripts were found in tissues with cell differentiation activities such as vascular axes and during the development of zygotic and somatic embryos. These results suggest that CchSERK1 might have regulatory functions in cell differentiation and could be used as a biotechnological tool to study the recalcitrance of peppers to proliferate in vitro.
Descritores: Capsicum/genética
Clonagem Molecular
-Técnicas In Vitro
Biotecnologia
Expressão Gênica
Diferenciação Celular
Genes de Plantas
DNA Complementar/genética
Solanaceae/genética
Proteínas de Arabidopsis
Proliferação de Células
Desenvolvimento Embrionário
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-989438
Autor: Long, Y L; Qiao, F; Jiang, X F; Cong, H Q; Sun, M L; Xu, Z J.
Título: Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile / Triagem e análise da expressão diferencial dos genes entre melancia diplóide e autotetraplóide através do perfil de expressão gênica digital
Fonte: Braz. j. biol;79(2):180-190, Apr.-June 2019. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Key Technologies Program of Haikou; . Science Research Foundation for High Education by Educational Commission of Hainan Province.
Resumo: Abstract Synthetic polyploids are key breeding materials for watermelon. Compared with diploid watermelon, the tetraploid watermelon often exhibit wide phenotypic differences and differential gene expression. Digital gene expression (DGE) profile technique was performed in this study to present gene expression patterns in an autotetraploid and its progenitor diploid watermelon, and deferentially expressed genes (DEGs) related to the abiotic and biotic stress were also addressed. Altogether, 4,985 DEGs were obtained in the autotetraploid against its progenitor diploid, and 66.02% DEGs is up-regulated. GO analysis shows that these DEGs mainly distributed in 'metabolic process', 'cell' and 'catalytic activity'. KEGG analysis revealed that these DEGs mainly cover 'metabolic pathways', 'secondary metabolites' and 'ribosome'. Moreover, 134 tolerance related DEGs were identified which cover osmotic adjustment substance, protective enzymes/protein, signaling proteins and pathogenesis-related proteins. This study present the differential expression of stress related genes and global gene expression patterns at background level in autotetraploid watermelons. These new evidences could supplement the molecular theoretical basis for the better resistance after the genome doubling in the gourd family.

Resumo Poliploides sintéticos são materias fundamentais para melhoramento genético da melancia. Comparativamente ao seu homólogo diploide, a melancia tetraploide apresenta amplas diferenças genotípica e fenotípica e diferença de expressão gênica. A expressão gênica digital ou DGE (digital gene expression) foi utilizada neste estudo para representar o perfil de expressão gênica da melancia autotetraploide e seu progenitor diploide e a expressão diferencial de genes relacionados ao estresse biótico e abiótico. Os resultados mostraram que 4.985 DEGs foram observados no organismo autotetraploide, sendo que, deste total, 66.02%foram supra-regulados. A análise de ontologia gênica (GO) mostrou que estes DEGs estão relacionados principalmente com processos metabólicas, célula e atividade catalítica, abrangendo de acordo com a análise de genes e genoma (KEGG) rotas metabólicas, metabolismo secundário e ribossomos. Além disso, 134 genes de defesa foram identificados, abrangendo substâncias de ajuste osmótico, enzimas/proteínas de proteção, proteínas sinalizadoras e proteínas relacionadas à patogênese. Este estudo mostrou a expressão diferencial de genes relacionados ao estresse e o perfil global de expressão gênica de melancia autotetraploide, estes resultados podem complementar, a nível molecular, o entendimento do fator resistência após a duplicação do genoma em cucurbitáceas.
Descritores: Poliploidia
Genes de Plantas/genética
Regulação da Expressão Gênica de Plantas/genética
Citrullus/genética
Citrullus/metabolismo
Transcriptoma/genética
-Perfilação da Expressão Gênica
Diploide
Responsável: BR1.1 - BIREME


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Id: biblio-1022130
Autor: Zhou, Shushan; Jiang, Li; Guan, Shuangxue; Gao, Yongxia; Gao, Qinghua; Wang, Guangdong; Duan, Ke.
Título: Expression profiles of five FT-like genes and functional analysis of PhFT-1 in a Phalaenopsis hybrid
Fonte: Electron. j. biotechnol;31:75-83, Jan. 2018. tab, ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Background: Phalaenopsis is an important ornamental flowering plant that belongs to the Orchidaceae family and is cultivated worldwide. Phalaenopsis has a long juvenile phase; therefore, it is important to understand the genetic elements regulating the transition from vegetative phase to reproductive phase. In this study, FLOWERING LOCUS T (FT) homologs in Phalaenopsis were cloned, and their effects on flowering were analyzed. Results: A total of five FT-like genes were identified in Phalaenopsis. Phylogenetic and expression analyses of these five FT-like genes indicated that some of these genes might participate in the regulation of flowering. A novel FT-like gene, PhFT-1, distantly related to previously reported FT genes in Arabidopsis and other dicot crops, was also found to be a positive regulator of flowering as heterologous expression of PhFT-1 in Arabidopsis causes an early flowering phenotype. Conclusions: Five FT homologous genes from Phalaenopsis orchid were identified, and PhFT-1 positively regulates flowering.
Descritores: Proteínas de Plantas/genética
Arabidopsis
Orchidaceae/genética
Flores/genética
-Reação em Cadeia da Polimerase/métodos
Clonagem Molecular
Genes de Plantas/genética
Biologia Computacional
Orchidaceae/crescimento & desenvolvimento
Flores/crescimento & desenvolvimento
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1017082
Autor: Mei, Lanju; Dong, Na; Li, Fosheng; Li, Na; Yao, Min; Chen, Fang; Tang, Lin.
Título: Transcriptome analysis of female and male flower buds of Idesia polycarpa Maxim. var. vestita Diels
Fonte: Electron. j. biotechnol;29:39-46, sept. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Science and Technology support projects of Sichuan Province.
Resumo: Background: Idesia polycarpa Maxim. var. vestita Diels, a dioecious plant, is widely used for biodiesel due to the high oil content of its fruits. However, it is hard to distinguish its sex in the seedling stage, which makes breeding and production problematic as only the female tree can produce fruits, and the mechanisms underlying sex determination and differentiation remain unknown due to the lack of available genomic and transcriptomic information. To begin addressing this issue, we performed the transcriptome analysis of its female and male flower. Results: 28,668,977 and 22,227,992 clean reads were obtained from the female and male cDNA libraries, respectively. After quality checks and de novo assembly, a total of 84,213 unigenes with an average length of 1179 bp were generated and 65,972 unigenes (78.34%) could be matched in at least one of the NR, NT, Swiss-Prot, COG, KEGG and GO databases. Functional annotation of the unigenes uncovered diverse biological functions and processes, including reproduction and developmental process, which may play roles in sex determination and differentiation. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed many unigenes annotated as metabolic pathways, biosynthesis of secondary metabolites pathways, plant­ pathogen interaction, and plant hormone signal transduction. Moreover, 29,953 simple sequence repeats were identified using the microsatellite software. Conclusion: This work provides the first detailed transcriptome analysis of female and male flower of I. polycarpa and lays foundations for future studies on the molecular mechanisms underlying flower bud development of I. polycarpa.
Descritores: Reprodução/genética
Salicaceae/genética
Transcriptoma
-Análise de Sequência de RNA
Genes de Plantas
Repetições de Microssatélites
Salicaceae/crescimento & desenvolvimento
Bases de Dados Genéticas
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1015856
Autor: He, Meijing; Cui, Shunli; Yang, Xinlei; Mu, Guojun; Chen, Huanying; Liu, Lifeng.
Título: Selection of suitable reference genes for abiotic stress-responsive gene expression studies in peanut by real-time quantitative PCR
Fonte: Electron. j. biotechnol;28:76-86, July. 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . 948 Project; . Special Fund for Modern Agro-industry Technology Research System of China; . Key Technologies Research and Development Program of Hebei Province.
Resumo: Background: Because of its strong specificity and high accuracy, real-time quantitative PCR (RT-qPCR) has been a widely used method to study the expression of genes responsive to stress. It is crucial to have a suitable set of reference genes to normalize target gene expression in peanut under different conditions using RT-qPCR. In this study, 11 candidate reference genes were selected and examined under abiotic stresses (drought, salt, heavy metal, and low temperature) and hormone (SA and ABA) conditions as well as across different organ types. Three statistical algorithms (geNorm, NormFinder and BestKeeper) were used to evaluate the expression stabilities of reference genes, and the comprehensive rankings of gene stability were generated. Results: The results indicated that ELF1B and YLS8 were the most stable reference genes under PEG-simulated drought treatment. For high-salt treatment using NaCl, YLS8 and GAPDH were the most stable genes. Under CdCl2 treatment, UBI1 and YLS8 were suitable as stable reference genes. UBI1, ADH3, and ACTIN11 were sufficient for gene expression normalization in low-temperature experiment. All the 11 candidate reference genes showed relatively high stability under hormone treatments. For organs subset, UBI1, GAPDH, and ELF1B showed the maximum stability. UBI1 and ADH3 were the top two genes that could be used reliably in all the stress conditions assessed. Furthermore, the necessity of the reference genes screened was further confirmed by the expression pattern of AnnAhs. Conclusions: The results perfect the selection of stable reference genes for future gene expression studies in peanut and provide a list of reference genes that may be used in the future.
Descritores: Arachis/genética
Genes de Plantas
Reação em Cadeia da Polimerase em Tempo Real/métodos
-Reguladores de Crescimento de Planta
Padrões de Referência
Seleção Genética
Estresse Fisiológico
Amplificação de Genes
Temperatura Baixa
Perfilação da Expressão Gênica
Secas
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Saúde Pública
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Id: lil-761805
Autor: Irrazábal, Gabriela.
Título: Religión y salud: la intervención pública de agentes religiosos católicos formados en bioética en el debate parlamentario sobre la muerte digna en la Argentina / Religion and health: the public intervention of Catholic religious agents trained in bioethics in the parliamentary debate on death with dignity in Argentina
Fonte: Salud colect;11(3):331-349, jul.-sep. 2015.
Idioma: es.
Resumo: Desde una perspectiva sociológica, este trabajo aborda una de las aristas de la intervención pública de ciertos sectores del catolicismo en la elaboración y sanción de leyes de salud. En particular se hace foco en el debate en comisiones parlamentarias sobre la llamada ley de "muerte digna" (Ley 26742) en el cual se convocó a un grupo de expertos en bioética para asesorar a los senadores sobre los alcances y límites de la ley. La mayoría de los expertos invitados pregonan la perspectiva de la bioética personalista, un desarrollo teológico de la bioética del catolicismo contemporáneo. En el debate no participaron representantes de otros credos consolidando la ampliamente estudiada imbricación entre el catolicismo y lo político en Argentina.

This paper discusses from a sociological perspective one of Catholicism's fronts of public intervention in the development and enactment of health legislation. In particular we analyze the debate in parliamentary committees on the so-called "death with dignity" law (No. 26742), for which a group of bioethics experts was convened to counsel senators regarding the scope and limits of the law. The majority of the invited experts advocated a personalist bioethics perspective, which is a theological bioethics development of contemporary Catholicism. In the debate no representatives of other faiths were present, reinforcing the widely studied overlap between Catholicism and politics in Argentina.
Descritores: Mesembryanthemum/fisiologia
RNA de Plantas/genética
Salinidade
Análise de Sequência de RNA
-Arabidopsis/efeitos dos fármacos
Arabidopsis/crescimento & desenvolvimento
Genes de Plantas
Mesembryanthemum/genética
Raízes de Plantas/crescimento & desenvolvimento
Cloreto de Sódio/farmacologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-757058
Autor: Musso, Carlos G; Enz, Paula A.
Título: El arte como instrumento para comprender la diferencia entre información, conocimiento y saber / Art as an instrument to understand the difference between information, knowledge and knowing
Fonte: Arch. argent. pediatr;113(5):388-389, oct. 2015.
Idioma: es.
Descritores: Cruzamento
Embaralhamento de DNA
Lilium/genética
Transformação Genética
-Clonagem Molecular
Flores/genética
Regulação da Expressão Gênica de Plantas
Redes Reguladoras de Genes
Genes de Plantas
Regeneração
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Texto completo SciELO Costa Rica
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Id: lil-753716
Autor: Minghui, Fu; Lihua, Jiang; Yuanmei, Li; Guohua, Yan; Lijun, Zheng; Jinping, Peng.
Título: Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) / Identificación de fragmentos de genes relacionados con la deficiencia de nitrógeno en Eichhornia crassipes (Pontederiaceae)
Fonte: Rev. biol. trop;62(4):1637-1648, oct.-dic. 2014. graf, tab.
Idioma: en.
Resumo: Eichhornia crassipes is an aquatic plant native to the Amazon River Basin. It has become a serious weed in freshwater habitats in rivers, lakes and reservoirs both in tropical and warm temperate areas worldwide. Some research has stated that it can be used for water phytoremediation, due to its strong assimilation of nitro- gen and phosphorus, and the accumulation of heavy metals, and its growth and spread may play an important role in environmental ecology. In order to explore the molecular mechanism of E. crassipes to responses to nitrogen deficiency, we constructed forward and reversed subtracted cDNA libraries for E. crassipes roots under nitrogen deficient condition using a suppressive subtractive hybridization (SSH) method. The forward subtraction included 2 100 clones, and the reversed included 2 650 clones. One thousand clones were randomly selected from each library for sequencing. About 737 (527 unigenes) clones from the forward library and 757 (483 unigenes) clones from the reversed library were informative. Sequence BlastX analysis showed that there were more transporters and adenosylhomocysteinase-like proteins in E. crassipes cultured in nitrogen deficient medium; while, those cultured in nitrogen replete medium had more proteins such as UBR4-like e3 ubiquitin- protein ligase and fasciclin-like arabinogalactan protein 8-like, as well as more cytoskeletal proteins, including actin and tubulin. Cluster of Orthologous Group (COG) analysis also demonstrated that in the forward library, the most ESTs were involved in coenzyme transportation and metabolism. In the reversed library, cytoskeletal ESTs were the most abundant. Gene Ontology (GO) analysis categories demonstrated that unigenes involved in binding, cellular process and electron carrier were the most differentially expressed unigenes between the forward and reversed libraries. All these results suggest that E. crassipes can respond to different nitrogen status by efficiently regulating and controlling some transporter gene expressions, certain metabolism processes, specific signal transduction pathways and cytoskeletal construction.

Se ha convertido en una maleza importante en hábitats de agua dulce en ríos, lagos y embalses, tanto en zonas tropicales como templadas de todo el mundo. Algunas investigaciones han indicado que se puede utilizar para la fitorremediación de agua, debido a su fuerte asimilación de nitrógeno y fósforo, y la acumulación de metales pesados, su crecimiento y propagación puede desempeñar un papel importante en la ecología ambiental. Con el fin de explorar el mecanismo molecular de respuesta a la deficiencia de nitrógeno en E. crassipes, se construyeron bibliotecas de cDNA mediante síntesis adelantada y retrasada para raíces de E. crassipes en condiciones de deficiencia de nitrógeno mediante el método de hibridación supresiva sustractiva (SSH). Para este estudio se utilizaron 2 100 clones de síntesis adelantada y 2 650 de síntesis retrasada. De la biblioteca se escogieron al azar mil clones, 737 (527 unigenes) de síntesis adelanta- da y 757 (483 unigenes) de síntesis retrasada que fueron informativos. El análisis BLASTX mostró que había más transportadores y proteínas adenosilhomocisteinasa en E. crassipes cultivadas en un medio deficiente de nitrógeno; mientras que las cultivadas en un medio repleto de nitróge- no tenían más proteínas como UBR4 e3 ubiquitina-proteína ligasa y la proteína arabinogalactano 8 tipo fasciclina, así como otras proteínas del citoesqueleto, incluyendo la actina y la tubulina. Clúster del Grupo Ortológico (COG) también demostró que en la biblioteca de síntesis adelan- tada, la mayoría de los marcadores de secuencia expresada (ESTs) estaban involucrados en el transporte de coenzimas y el metabolismo.
Descritores: Recuperação e Remediação Ambiental
Etiquetas de Sequências Expressas
Eichhornia/genética
Genes de Plantas
Nitrogênio/metabolismo
Fósforo/metabolismo
-Eichhornia/classificação
Eichhornia/metabolismo
Biblioteca Gênica
Nitrogênio/deficiência
Reação em Cadeia da Polimerase
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Texto completo SciELO Brasil
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Id: lil-695247
Autor: Mendes, A.D.R.; Martins, E.R.; Figueiredo, L.S.
Título: Estudo do sistema de reprodução da fava-d'anta (Dimorphandra mollis Benth. ) / Determination of the reproductive system of fava-d'anta (Dimorphandra mollis Benth. )
Fonte: Rev. bras. plantas med;15(4):605-608, 2013. tab.
Idioma: pt.
Resumo: Entre as plantas nativas de uso medicinal do Cerrado brasileiro encontra-se a fava-d'anta (Dimorphandra mollis Benth) com alto potencial econômico por possuir inúmeras potencialidades medicinais e fitoquímicas. A indústria extrai dos frutos os princípios ativos rutina, quercetina, e ramnose, dentre outros, usados na fabricação de medicamentos e cosméticos, principalmente no exterior. O conhecimento do sistema reprodutivo da espécie é fundamental para sua conservação e manejo. O presente trabalho objetivou determinar as características morfométricas das flores e caracterizar o sistema reprodutivo da D. mollis em área de Cerrado inalterada antropomorficamente, no município de Olhos D'água - MG. Para as características morfométricas, diâmetro da flor, comprimento da flor, do ovário, e da antera, foram utilizadas cinco flores em pré-antese. Para a determinação do sistema reprodutivo utilizou-se a razão pólen:óvulo (P:O), em 40 flores. As flores da D. mollis apresentaram comprimento da flor de 3,00 mm, diâmetro da flor de 2,00 mm, comprimento do óvulo de 2,60 mm, comprimento da antera de 1,57 mm, e o número de óvulos e de anteras foram 20 e 5,8, respectivamente. A razão P:O foi 765,030, sendo que esse índice não é afetado pelas características morfométricas. O sistema reprodutivo da D. mollis foi classificado como alógama facultativa.

Among the native medicinal plants of the Brazilian Cerrado, we can find the fava-d'anta (Dimorphandra mollis Benth) with a high economic potencial due to its numerous possivbilities for medicinal and phytochemical use. The industry extracts form the fruits the active ingredients rutin, quercetin and rhamnose, among others, used to manufacture medications and cosmetic products, especially abroad. Knowing the reproductive system of the species is important for its conservation and management. This study aimed to determine the morphometric characteristics of flowers and characterize the reproductive system of D. mollis in a Cerrado area that was anthropomorphically unchanged, in the municipality of Olhos D'água - MG. For the morphometric characteristics, flower diameter, length of the flower, of the ovary and the anther, five pre-anthesis flowers were used. To determine the reproductive system, the pollen:ovule (P:O) ratio was used in 40 flowers. The D. mollis flowers presented a flower length of 3.00 mm, flower diameter of 2.00 mm, ovule length of 2.60 mm, anthers length of 1.57 mm, and the number of anthers and ovules were 20 and 5.8, respectively. The P:O ratio was 765,030, and this index is not affected by the morphometric characteristics. The reproductive system of D. mollis was classified as facultative allogamous.
Descritores: Fabaceae/classificação
Plantas Medicinais/genética
Reprodução/genética
-Genes de Plantas
Pradaria
Responsável: BR1.1 - BIREME



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