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Id: biblio-976235
Autor: Cassu-Corsi, Dandara; Martins, Willames MBS; Nicoletti, Adriana G; Almeida, Luiz GP; Vasconcelos, Ana TR; Gales, Ana C.
Título: Characterisation of plasmid-mediated rmtB-1 in Enterobacteriaceae clinical isolates from São Paulo, Brazil
Fonte: Mem. Inst. Oswaldo Cruz;113(12):e180392, 2018. tab, graf.
Idioma: en.
Projeto: CAPES; . CNPq.
Resumo: OBJECTIVES The emergence of 16S rRNA methyltranferases (16 RMTAses) has jeopardised the clinical use of aminoglycosides. RmtB is one of the most frequently reported in Gram-negatives worldwide. In this study, we aimed to estimate the frequency of 16S RMTAses encoding genes in Enterobacteriaceae isolated in a three-month period from a tertiary Brazilian hospital. METHODS All Gram-negatives classified as resistant to amikacin, gentamicin, and tobramycin by agar screening were selected for analysis. The presence of 16SRMTases encoding genes was verified by polymerase chain reaction (PCR). Antimicrobial susceptible profile was determined by broth microdilution. The genetic relationship among these isolates was accessed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Selected RmtB-producing isolates were characterised by whole genome sequencing (WGS) analysis. RESULTS Twenty-two of 1,052 (2.1%) Enterobacteriaceae were detected as producers of RmtB-1 [Klebsiella pneumoniae (n = 21) and Proteus mirabilis (n = 1)]. blaKPC-2 was identified among 20 RmtB-1-producing K. pneumoniae isolates that exhibited an identical PFGE and MLST (ST258) patterns. Two K. pneumoniae isolates, the A64216 (not harboring bla KPC-2), A64477 (harboring bla KPC-2) and one P. mirabilis isolate (A64421) were selected for WGS. rmtB-1 and bla KPC-2 genes were carried by distinct plasmids. While a plasmid belonging to the IncFIIk group harbored rmtB-1 in K. pneumoniae, this gene was carried by a non-typable plasmid in P. mirabilis. In the three analysed plasmids, rmtB-1 was inserted on a transposon, downstream a Tn2. CONCLUSION Our findings suggested that the rmtB-1 was harbored by plasmids distinct from those previously reported in Bolivia and China. It suggests that multiple mobilization events might have occurred in South America.
Descritores: Surtos de Doenças/estatística & dados numéricos
Enterobacteriaceae
Klebsiella pneumoniae
-Genes de RNAr/genética
Aminoglicosídeos/uso terapêutico
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-536332
Autor: Nirchio, Mauro; Oliveira, Claudio; Ferreira, Daniela C; Rondón, Rodolfo; Pérez, Julio E; Hett, Anne Kathrin; Rossi, Anna Rita; Sola, Luciana.
Título: Cytogenetic characterization of Rhomboplites aurorubens and Ocyurus chrysurus, two monotypic genera of Lutjaninae from Cubagua Island, Venezuela, with a review of the cytogenetics of Lutjanidae (Teleostei: Perciformes)
Fonte: Neotrop. ichthyol;7(4):587-594, 2009. tab, ilus.
Idioma: en.
Resumo: Lutjanidae, commonly known as snappers, includes 105 species, grouped in four subfamilies. In spite of the high number of species and of its worldwide distribution, the family has been little investigated and the phylogenetic relationships among some of its genera and species are still cause for debate. Only a small number of the species has been cytogenetically analysed. This study reports the first description of the karyotype of Rhomboplites aurorubens as well as data concerning the distribution of the constitutive heterochromatin and the location of the 18S rRNA and the 5S rRNA genes. Specimens of Ocyurus chrysurus from Venezuela were also investigated for the same cytogenetic features. Both species have a 48 uniarmed karyotype, but R. aurorubens has a single subtelocentric chromosome pair, the smallest of the chromosome complement, among the other acrocentric chromosomes. The C-positive heterochromatin is limited to the pericentromeric regions of all chromosomes. Both species show a single chromosome pair bearing the Nucleolus Organizer Regions, but NORs are differently located, in a terminal position on the short arms of the smallest chromosomes in R. aurorubens and in a paracentromeric position in a chromosome pair of large size in O. chrysurus. In O. chrysurus, the 5S rDNA gene cluster is located on a medium-sized chromosome pair, whereas in R. aurorubens it is syntenic with the 18S rDNA gene cluster on chromosome pair number 24. The obtained cytogenetic data, along with previous cytogenetic, morphological and molecular data for the family, reinforce the proposal to synonymize genus Ocyurus with Lutjanus. A review of Lutjanidae cytogenetics is also included.

Lutjanidae, comumente conhecidos como snappers, inclui 105 espécies, reunidas em quatro subfamílias. A despeito do grande número de espécies e de sua distribuição mundial, a família tem sido pouco estudada e as relações filogenéticas entre alguns de seus gêneros e espécies ainda é motivo de debates. Apenas um pequeno número de espécies foi citogeneticamente analisada. Esse estudo apresenta a primeira descrição do cariótipo de Rhomboplites aurorubens assim como dados relativos à distribuição de heterocromatina constitutiva e localização dos genes 18S rRNA e 5S rRNA. Espécimes de Ocyurus chrysurus da Venezuela foram também analisados quanto às mesmas características citogenéticas. Ambas as espécies têm cariótipos compostos de 48 cromossomos com um único braço, entretanto R. aurorubens tem um único par de cromossomos subtelocêntrico, o menor do complemento cromossômico, entre os outros cromossomos acrocêntricos. A heterocromatina C-positiva é limitada à região pericentromérica de todos os cromossomos. Ambas as species apresentam um único par com Regiões Organizadoras de Nucléolo, mas as RONs são localizadas em posições diferentes, em posição terminal no braço curto dos menores cromossomos de R. aurorubens e em posição paracentromérica no braço longo de um par de cromossomos grandes de O. chrysurus. Em O. chrysurus, os genes 5S rDNA estão localizados em um par de cromossomos de tamanho médio, enquanto em R. aurorubens eles são sintenicamente localizados com os genes 18S rDNA no par de cromossomos número 24. Os dados citogenéticos obtidos, junto com os dados morfológicos e moleculares disponíveis para a família reforçam a proposta de sinonimizar o gênero Ocyurus com Lutjanus. Uma revisão da citogenética dos Lutjanidae é também apresentada
Descritores: Perciformes/genética
Citogenética/classificação
-Heterocromatina
Genes de RNAr/genética
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-969565
Autor: Sousa, C. P; Soares, J. G. B.
Título: Employment of the 18s rRNA screening PCR technique in the detection of Equine Piroplasmosis, in horses of sports and military operations, of the Brazilian Army / Emprego da técnica de PCR para triagem da região do genoma 18s rRNA, detecção de Piroplasmose em equinos de desporto e operações militares do Exército Brasileiro
Fonte: Arq. bras. med. vet. zootec. (Online);70(6):1680-1684, nov.-dez. 2018. tab, ilus.
Idioma: en.
Resumo: The present work had the objective of detecting the occurrence of Equine Piroplasmosis in horses housed in the 3rd Guards Cavalry Regiment (GCR) - Brazilian Army (BA) Ë— Porto Alegre, RS-Brazil, as well as to demonstrate the proactivity of PCR (Polymerase Chain Reaction) technique, aiming at the judicious use of the resources involved in the training and employment of Equines in the Brazilian Army. Fifty horses of the 3rd GCR - Porto Alegre Ë— RS, which are employed for Sport, Military Ceremonial, Law and Order Guarantee Operations (LOGO), were evaluated by means of the 18s r RNA screening with PCR technique, thirty eight horses with Babesia Caballi and Theileria Equi were detected, which corresponds to an incidence of 76% of the horses effective analyzed at the time. In this way, it can be verified that the Military activity have its "performance and effectiveness" factors threatened in case the health of the principal of his means employed, that is the horse, is compromised. The PCR technique then offers a reliable and feasible tool for the detection of Equine Piroplasmosis in BA horses.(AU)

O presente trabalho teve como objetivo detectar a ocorrência de Piroplasmose equina em cavalos alojados no 3º Regimento de Cavalaria de Guarda (RCG) - Exército Brasileiro (EB) - Porto Alegre, RS, Brasil, bem como demonstrar a forma proativa do método da PCR (reação em cadeia de polimerase), objetivando o uso criterioso dos recursos envolvidos no treinamento e emprego de equinos no Exército Brasileiro. Foram avaliados 50 cavalos da 3ª GCR-Porto Alegre, RS, empregados nas modalidades de: esporte, cerimonial militar e operações de garantia da lei e da ordem (GLO), por meio da triagem da região do genoma 18S rRNA mediante a aplicação do método da PCR. Foram positivas as amostras de 38 equinos para Babesia caballi e Theileria Equi, o que corresponde a uma incidência de 76% dos cavalos efetivos analisados na época. Dessa forma, verifica-se que as atividades militares tem seus fatores de "desempenho e efetividade" ameaçados no caso da saúde do principal de seus meios empregados, o Cavalo, estar comprometida. A técnica de PCR, então, oferece uma ferramenta confiável e viável para a detecção de Piroplasmose em equinos do EB.(AU)
Descritores: Babesiose/diagnóstico
Reação em Cadeia da Polimerase/estatística & dados numéricos
Genes de RNAr
Cavalos/anormalidades
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-794994
Autor: Senthilraj, Rajapandi; Prasad, Ganduri Sathyanarayana; Janakiraman, Kunchithapatham.
Título: Sequence-based identification of microbial contaminants in non-parenteral products
Fonte: Braz. j. pharm. sci;52(2):329-336, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Phenotypic profiles for microbial identification are unusual for rare, slow-growing and fastidious microorganisms. In the last decade, as a result of the widespread use of PCR and DNA sequencing, 16S rRNA sequencing has played a pivotal role in the accurate identification of microorganisms and the discovery of novel isolates in microbiology laboratories. The 16S rRNA region is universally distributed among microorganisms and is species-specific. Accordingly, the aim of our study was the genotypic identification of microorganisms isolated from non-parenteral pharmaceutical formulations. DNA was separated from five isolates obtained from the formulations. The target regions of the rRNA genes were amplified by PCR and sequenced using suitable primers. The sequence data were analyzed and aligned in the order of increasing genetic distance to relevant sequences against a library database to achieve an identity match. The DNA sequences of the phylogenetic tree results confirmed the identity of the isolates as Bacillus tequilensis, B. subtilis, Staphylococcus haemolyticus and B. amyloliqueficians. It can be concluded that 16S rRNA sequence-based identification reduces the time by circumventing biochemical tests and also increases specificity and accuracy.

RESUMO Os perfis fenotípicos para identificação microbiana são incomuns para micro-organismos raros, de crescimento lento e exigentes. Na última década, em resultado do uso generalizado de PCR e sequenciação de DNA, a sequenciação do rRNA 16S tem desempenhado papel crucial na identificação precisa do micro-organismo e a descoberta de novos isolados em laboratórios de microbiologia. A região de rRNA 16S é universalmente distribuída entre micro-organismos e é espécie-específica. A genotipagem foi realizada sobre os organismos isolados a partir de formulações farmacêuticas não parenterais. O DNA foi separado dos cinco isolados obtidos a partir das formulações. As regiões alvo dos genes de rRNA foram amplificados por PCR e sequenciados utilizando os iniciadores adequados. Os dados dos sequência foram analisados e alinhados na ordem crescente de distância genética de sequências relevantes contra biblioteca de dados para obter a identidade. A sequência de DNA de árvores filogenéticas confirma a identidade dos isolados como Bacillus-tequilensis, B. subtilis, Staphylococcus haemolyticus e B. amyloliqueficians. Pode-se concluir identificação baseada na sequência do rRNA 16S reduz o tempo por evitar testes bioquímicos e também aumenta a especificidade e a precisão.
Descritores: Técnicas Microbiológicas/análise
Análise de Sequência de DNA/métodos
-Genes Microbianos
Genes de RNAr
Responsável: BR1.1 - BIREME


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Id: lil-749738
Autor: Khan, Shamiyan R.; Nirmal, J.I. Kumar; Kumar, Rita N.; Patel, Jignasha G..
Título: Biodegradation of kerosene: Study of growth optimization and metabolic fate of P. janthinellum SDX7
Fonte: Braz. j. microbiol;46(2):397-406, Apr-Jun/2015. tab, graf.
Idioma: en.
Resumo: Penicillum janthinellum SDX7 was isolated from aged petroleum hydrocarbon-affected soil at the site of Anand, Gujarat, India, and was tested for different pH, temperature, agitation and concentrations for optimal growth of the isolate that was capable of degrading upto 95%, 63% and 58% of 1%, 3% and 5% kerosene, respectively, after a period of 16 days, at optimal growth conditions of pH 6.0, 30 °C and 180 rpm agitation. The GC/MS chromatograms revealed that then-alkane fractions are easily degraded; however, the rate might be lower for branched alkanes, n-alkylaromatics, cyclic alkanes and polynuclear aromatics. The test doses caused a concentration-dependent depletion of carbohydrates of P. janthinellum SDX7 by 3% to 80%, proteins by 4% to 81% and amino acids by 8% to 95% upto 16 days of treatment. The optimal concentration of 3% kerosene resulted in the least reduction of the metabolites of P. janthinellum such as carbohydrates, proteins and amino acids with optimal growth compared to 5% and 1% (v/v) kerosene doses on the 12th and 16th day of exposure. Phenols were found to be mounted by 43% to 66% at lower and higher concentrations during the experimental period. Fungal isolate P. janthinellum SDX7 was also tested for growth on various xenobiotic compounds.
Descritores: Querosene
Penicillium/crescimento & desenvolvimento
Penicillium/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
Xenobióticos/metabolismo
-Composição de Bases
Biotransformação
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Cromatografia Gasosa-Espectrometria de Massas
Genes de RNAr
Concentração de Íons de Hidrogênio
Índia
Dados de Sequência Molecular
Penicillium/genética
Penicillium/isolamento & purificação
RNA Fúngico/genética
/genética
RNA, RIBOSOMAL, 1ABDOMINAL NEOPLASMSS/genética
Análise de Sequência de DNA
Temperatura
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-703648
Autor: Costa, Daniela Camargos; Madureira, Ana Paula; Amaral, Lara Cotta; Sanchez, Bruno Antonio Marinho; Gomes, Luciano Teixeira; Fontes, Cor Jesus Fernandes; Limongi, Jean Ezequiel; Brito, Cristiana Ferreira Alves de; Carvalho, Luzia Helena.
Título: Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods?
Fonte: Mem. Inst. Oswaldo Cruz;109(1):21-28, 02/2014. tab, graf.
Idioma: en.
Resumo: The polymerase chain reaction (PCR)-based methods for the diagnosis of malaria infection are expected to accurately identify submicroscopic parasite carriers. Although a significant number of PCR protocols have been described, few studies have addressed the performance of PCR amplification in cases of field samples with submicroscopic malaria infection. Here, the reproducibility of two well-established PCR protocols (nested-PCR and real-time PCR for the Plasmodium 18 small subunit rRNA gene) were evaluated in a panel of 34 blood field samples from individuals that are potential reservoirs of malaria infection, but were negative for malaria by optical microscopy. Regardless of the PCR protocol, a large variation between the PCR replicates was observed, leading to alternating positive and negative results in 38% (13 out of 34) of the samples. These findings were quite different from those obtained from the microscopy-positive patients or the unexposed individuals; the diagnosis of these individuals could be confirmed based on the high reproducibility and specificity of the PCR-based protocols. The limitation of PCR amplification was restricted to the field samples with very low levels of parasitaemia because titrations of the DNA templates were able to detect < 3 parasites/µL in the blood. In conclusion, conventional PCR protocols require careful interpretation in cases of submicroscopic malaria infection, as inconsistent and false-negative results can occur.
Descritores: Portador Sadio/parasitologia
DNA de Protozoário/análise
Malária/parasitologia
Plasmodium/genética
Reação em Cadeia da Polimerase/métodos
-Distribuição de Qui-Quadrado
Portador Sadio/diagnóstico
Coinfecção/diagnóstico
Genes de RNAr/genética
Microscopia
Malária/diagnóstico
Parasitemia/diagnóstico
Parasitemia/parasitologia
Plasmodium/classificação
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sensibilidade e Especificidade
Limites: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-699830
Autor: Qiu, Shanlian; Wang, MK; Wang, Fei; Chen, Jichen; Li, Xiaoyan; Li, Qinghua; Lin, Cheng; Lin, Xinjian.
Título: Effects of open drainage ditch design on bacterial and fungal communities of cold waterlogged paddy soils
Fonte: Braz. j. microbiol;44(3):983-991, July-Sept. 2013. ilus, graf, tab.
Idioma: en.
Projeto: China. Agro-scientific Research; . China. Fujian Academy of Agricultural Sciences; . China. Fujian Province.
Resumo: A field experiment established in 1980 was conducted to evaluate the effects of open drainage ditch applied for water removal on bacterial and fungal communities of cold waterlogged paddy soils in 2011. In this experiment, traditional plate counting and temperature gradient gel electrophoresis were employed to characterize the abundance and diversity of soil bacterial and fungal communities. Four different distances from the open drainage ditch, 5, 15, 25 and 75 m with different degrees of drainage were designed for this study. Maximum populations of culturable aerobic bacteria and fungi were at 15-m distance while minimum populations were at 75-m distance. Significant differences (p < 0.05) in fungal populations were observed at all distances from open drainage ditch. The highest diversity of the bacterial community was found at a distance of 25 m, while that of the fungal community was observed at a distance of 5 m. Sequencing of excised TGGE bands indicated that the dominant bacteria at 75-m distance belonged to anaerobic or microaerobic bacteria. Relationships between microbial characteristics and soil physicochemical properties indicated that soil pH and available nitrogen contents were key factors controlling the abundance of culturable aerobic bacteria and fungi, while soil water capacity also affected the diversity of fungal community. These findings can provide the references for better design and advanced management of the drainage ditches in cold waterlogged paddy soils.
Descritores: Biota
Bactérias/classificação
Bactérias/isolamento & purificação
Fenômenos Químicos
Fungos/classificação
Fungos/isolamento & purificação
Microbiologia do Solo
-Análise por Conglomerados
Temperatura Baixa
Eletroforese em Gel de Gradiente Desnaturante
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Drenagem
Genes de RNAr
Concentração de Íons de Hidrogênio
Dados de Sequência Molecular
Nitrogênio/análise
Filogenia
RNA Bacteriano/genética
RNA Fúngico/genética
/genética
RNA, RIBOSOMAL, 1ABDOMINAL NEOPLASMSS/genética
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
Solo/química
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-670399
Autor: Memórias do Instituto Oswaldo Cruz; Taverna, Constanza Giselle; Bosco-Borgeat, María Eugenia; Murisengo, Omar Alejandro; Davel, Graciela; Boité, Mariana Côrtes; Cupolillo, Elisa; Canteros, Cristina Elena.
Título: Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
Fonte: Mem. Inst. Oswaldo Cruz;108(2):178-185, abr. 2013. tab, graf.
Idioma: en.
Resumo: As the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means of accurately and rapidly identifying these species is necessary for the successful early resolution of infection and the subsequent reduction of morbidity and mortality. The current work aimed to evaluate ribosomal RNA gene sequencing for the identification of medically relevant Candida species in comparison with a standard phenotypic method. Eighteen reference strains (RSs), 69 phenotypically identified isolates and 20 inconclusively identified isolates were examined. Internal transcribed spaces (ITSs) and D1/D2 of the 26S ribosomal RNA gene regions were used as targets for sequencing. Additionally, the sequences of the ITS regions were used to establish evolutionary relationships. The sequencing of the ITS regions was successful for 88% (94/107) of the RS and isolates, whereas 100% of the remaining 12% (13/107) of the samples were successfully analysed by sequencing the D1/D2 region. Similarly, genotypic analysis identified all of the RS and isolates, including the 20 isolates that were not phenotypically identified. Phenotypic analysis, however, misidentified 10% (7/69) of the isolates. Phylogenetic analysis allowed the confirmation of the relationships between evolutionarily close species. Currently, the use of genotypic methods is necessary for the correct identification of Candida species.
Descritores: Candida/genética
DNA Fúngico/análise
DNA Espaçador Ribossômico/genética
Genes de RNAr/genética
-Candida/classificação
Genótipo
Fenótipo
Reação em Cadeia da Polimerase
Análise de Sequência de RNA
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: lil-644472
Autor: Braga, M. S. C. O; André, M. R; Freschi, C. R; Teixeira, M. C. A; Machado, R. Z.
Título: Molecular detection of hemoplasma infection among cats from São Luís island, Maranhão, Brazil
Fonte: Braz. j. microbiol;43(2):569-575, Apr.-June 2012. ilus, tab.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Hemoplasmas are bacteria that infect erythrocytes, attaching to the red blood cell. There is a need for more reports of hemoplasma infection prevalence and molecular characterization among cats in Brazil since there are only few published reports. The present work aimed to detect and molecularly characterize the presence of hemotrophic mycoplasmas in domestic cats with outdoor access from São Luís, Maranhão, Brazil. Twenty cats (10%) were positive for Candidatus M. haemominutum, five (2.5%) for M. haemofelis, and four (2.%) for M. turicensis based on 16S rRNA gene PCRs. Five cats (2.5%) were co-positive for Candidatus M. haemominutum and M. haemofelis. PCR diagnosis was confirmed by sequencing; and phylogenetic analysis was based on 16S rRNA and rnpb genes.
Descritores: Análise de Sequência de DNA/métodos
Análise de Sequência de RNA/métodos
Gatos
Eritrócitos/patologia
Genes de RNAr
Técnicas In Vitro
Infecções por Mycoplasma
Mycoplasma/genética
Mycoplasma/isolamento & purificação
Filogenia
-Determinação
Métodos
Técnicas
Limites: Animais
Gatos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-551890
Autor: Tabatabaei, Meisam; Zakaria, Mohd Rafein; Rahim, Raha Abdul; Wright, André-Denis G; Shirai, Yoshihito; Abdullah, Norhani; Sakai, Kenji; Ikeno, Shinya; Mori, Masatsugu; Kazunori, Nakamura; Sulaiman, Alawi; Hassan, Mohd Ali.
Título: PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent
Fonte: Electron. j. biotechnol;12(3):12-13, July 2009. ilus, tab.
Idioma: en.
Resumo: 16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens, and a probe for bacteria. In situ hybridization with methanogens and bacterial probes and denatured gradient gel electrophoresis within activated sludge clearly confirmed the presence of Methanosaeta sp. and Methanosarcina sp. cells. Methanosaeta concilii was found to be the dominant species in the bioreactor. These results revealed the presence of possibly new strain of Methanosaeta in the bioreactor for treating palm oil mill effluent called Methanosaeta concilii SamaliEB (Gene bank accession number: EU580025). In addition, fluorescent hybridization pictured the close association between the methanogens and bacteria and that the number of methanogens was greater than the number of bacteria.
Descritores: Óleo de Palmeira/análise
Clonagem Molecular
Digestão Anaeróbia/análise
Genes de RNAr
Methanosarcina/isolamento & purificação
Methanosarcinales/isolamento & purificação
Óleo de Palmeira
Tanques Imhoff/análise
-Hibridização in Situ Fluorescente
Reação em Cadeia da Polimerase/métodos
Responsável: CL1.1 - Biblioteca Central



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde