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Pesquisa : G05.360.340.024.340.825 [Categoria DeCS]
Referências encontradas : 21 [refinar]
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Id: biblio-1253093
Autor: Umer, Noroza; Zahra Naqvi, Rubab; Rauf, Imran; Anjum, Naveed; Keen, Patricia R; Van Eck, Joyce; Jander, Georg; Asif, Muhammad.
Título: Expression of Pinellia ternata leaf agglutinin under rolC promoter confers resistance against a phytophagous sap sucking aphid, Myzus persicae
Fonte: Electron. j. biotechnol;47:72-82, sept. 2020. tab, ilus, graf.
Idioma: en.
Projeto: International Research Support Initiative Program of Higher Education Commission (HEC) of; . Pakistan; . United States Department of Agriculture (USDA).
Resumo: BACKGROUND: Piercing/sucking insect pests in the order Hemiptera causes substantial crop losses by removing photoassimilates and transmitting viruses to their host plants. Cloning and heterologous expression of plantderived insect resistance genes is a promising approach to control aphids and other sap-sucking insect pests. While expression from the constitutive 35S promoter provides broad protection, the phloem-specific rolC promoter provides better defense against sap sucking insects. The selection of plant-derived insect resistance genes for expression in crop species will minimize bio-safety concerns. RESULTS: Pinellia ternata leaf agglutinin gene (pta), encodes an insecticidal lectin, was isolated and cloned under the 35S and rolC promoters in the pGA482 plant transformation vector for Agrobacterium-mediated tobacco transformation. Integration and expression of the transgene was validated by Southern blotting and qRT-PCR, respectively. Insect bioassays data of transgenic tobacco plants showed that expression of pta under rolC promoter caused 100% aphid mortality and reduced aphid fecundity up to 70% in transgenic tobacco line LRP9. These results highlight the better effectivity of pta under rolC promoter to control phloem feeders, aphids. CONCLUSIONS: These findings suggested the potential of PTA against aphids and other sap sucking insect pests. Evaluation of gene in tobacco under two different promoters; 35S constitutive promoter and rolC phloemspecific promoter could be successfully use for other crop plants particularly in cotton. Development of transgenic cotton plants using plant-derived insecticidal, PTA, would be key step towards commercialization of environmentally safe insect-resistant crops.
Descritores: Afídeos/patogenicidade
Controle Biológico de Vetores
Pinellia/química
-Vírus de Plantas
Tabaco
Southern Blotting
Reação em Cadeia da Polimerase
Regiões Promotoras Genéticas
Plantas Geneticamente Modificadas
Folhas de Planta/química
Transgenes
Resistência à Doença
Proteção de Cultivos
Responsável: CL1.1 - Biblioteca Central


  2 / 21 LILACS  
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Texto completo SciELO Chile
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Id: biblio-950778
Autor: Muzaffar, Adnan; Kiani, Sarfraz; Khan, Muhammad Azmat Ullah; Rao, Abdul Qayyum; Ali, Arfan; Awan, Mudassar Fareed; Iqbal, Adnan; Nasir, Idrees Ahmad; Shahid, Ahmad Ali; Husnain, Tayyab.
Título: Chloroplast localization of Cry1Ac and Cry2A protein- an alternative way of insect control in cotton
Fonte: Biol. Res;48:1-11, 2015. ilus, tab.
Idioma: en.
Resumo: BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating the target protein at the point of insect attack will be more effective. It will not mean that the non-green parts of the plants are free of toxic proteins, but it will inflict more damage on the insects because they are at maximum activity in the green parts of plants. RESULTS: Successful cloning was achieved by the amplification of Cry2A, Cry1Ac, and a transit peptide. The appropriate polymerase chain reaction amplification and digested products confirmed that Cry1Ac and Cry2A were successfully cloned in the correct orientation. The appearance of a blue color in sections of infiltrated leaves after 72 hours confirmed the successful expression of the construct in the plant expression system. The overall transformation efficiency was calculated to be 0.7%. The amplification of Cry1Ac-Cry2A and Tp2 showed the successful integration of target genes into the genome of cotton plants. A maximum of 0.673 µg/g tissue of Cry1Ac and 0.568 µg/g tissue of Cry2A was observed in transgenic plants. We obtained 100% mortality in the target insect after 72 hours of feeding the 2nd instar larvae with transgenic plants. The appearance of a yellow color in transgenic cross sections, while absent in the control, through phase contrast microscopy indicated chloroplast localization of the target protein. CONCLUSION: Locating the target protein at the point of insect attack increases insect mortality when compared with that of other transgenic plants. The results of this study will also be of great value from a biosafety point of view.
Descritores: Proteínas de Bactérias/genética
Proteínas Recombinantes de Fusão
Cloroplastos/genética
Controle de Insetos/métodos
Gossypium/genética
Endotoxinas/genética
Proteínas Hemolisinas/genética
Lepidópteros
-Bacillus thuringiensis
Proteínas de Bactérias/análise
Resistência a Inseticidas/genética
Imuno-Histoquímica
Expressão Gênica/genética
Cloroplastos/metabolismo
Reação em Cadeia da Polimerase
Microscopia de Contraste de Fase
Plantas Geneticamente Modificadas
Clonagem Molecular
Primers do DNA
Folhas de Planta/genética
Transgenes/fisiologia
Endotoxinas/análise
Fusão Gênica
Proteínas Hemolisinas/análise
Inseticidas
Larva
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  3 / 21 LILACS  
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Id: lil-697834
Autor: Costa, Rodolfo; Stanewsky, Ralf.
Título: When population and evolutionary genetics met behaviour
Fonte: Mem. Inst. Oswaldo Cruz;108(supl.1):74-79, 2013. tab, graf.
Idioma: en.
Resumo: In this review, we analyse the impact of a population and evolutionary genetics approach on the study of insect behaviour. Our attention is focused on the model organism Drosophila melanogaster and several other insect species. In particular, we explore the relationship between rhythmic behaviours and the molecular evolution of clock and ion channel genes.
Descritores: Comportamento Animal/fisiologia
Relógios Circadianos/genética
Drosophila melanogaster/genética
Evolução Molecular
Genética Populacional
-Proteínas CLOCK/genética
Drosophila/genética
Especiação Genética
Canais Iônicos/genética
Proteínas Circadianas Period/genética
Psychodidae/genética
Comportamento Sexual Animal
Temperatura
Transgenes/genética
Limites: Animais
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  4 / 21 LILACS  
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Id: lil-695437
Autor: Pereyra-Bonnet, Federico.
Título: Plasticidad celular: convirtiendo una célula en otra / Cell plasticity: converting one cell into another
Fonte: Rev. Hosp. Ital. B. Aires (2004);32(4):188-190, dic. 2012. ilus.
Idioma: es.
Descritores: Fenômenos Fisiológicos Celulares
Células
Técnicas de Transferência de Genes
Células-Tronco
Transgenes
-Genética Médica/tendências
Organismos Geneticamente Modificados
Limites: Humanos
Responsável: AR2.1 - Biblioteca Central


  5 / 21 LILACS  
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Texto completo SciELO Chile
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Id: lil-646952
Autor: Hadi, Faranak; Salmanian, Ali Hatef; Ghazizadeh, Elham; Amani, Jafar; Noghabi, Kambiz Akbari; Mousavi, Amir.
Título: Development of quantitative competitive PCR for determination of copy number and expression level of the synthetic glyphosate oxidoreductase gene in transgenic canola plants
Fonte: Electron. j. biotechnol;15(4):2-2, July 2012. ilus, tab.
Idioma: en.
Resumo: Background: For successful in vitro plant regeneration, plant cell lines with multiple transgene integration and low transgene expression levels need to be ruled out. Although real-time polymerase chain reaction (RT-PCR) is a rapid way to accomplish this, it is also expensive and typically limits the size of the target sequence. Quantitative competitive PCR (QC-PCR) is proven to be a safe and accurate method for determination of both copy number and quantification of transcript levels of synthetic transgenes in transformed plants. Results: The glyphosate oxidoreductase genewas chemically synthesized and used to transform Brassica napus L. via Agrobactrium-mediated transformation. A construct containing the mutated form of a synthetic glyphosate oxidoreductase (gox) gene (internal standard) was prepared. Gene copy number was estimated in nine independent transgenic lines using QC-PCR as well as the standard method of Southern blot analysis. By quantitative RT-PCR, transcript levels were also determined in these lines. High (> 3), medium to high (2.2-3), medium to low (1-2.2), and low (< 1) levels of transcript were detected. Conclusions: No direct relationship was found between copy number and transgene expression levels. QC-PCR method could be implemented to screen putative transgenic plants and quickly select single T-DNA inserts. QC-PCR methods and the prepared competitor construct may be useful for future quantification of commercial transgenic food and feed.
Descritores: Brassica napus/enzimologia
Brassica napus/genética
Glicina/genética
Oxirredutases/genética
Reação em Cadeia da Polimerase/métodos
-Glicina/análogos & derivados
Glicina/fisiologia
Oxirredutases/fisiologia
Transcrição Genética
Transgenes
Responsável: CL1.1 - Biblioteca Central


  6 / 21 LILACS  
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Id: lil-640504
Autor: Gamarra, Luis Fernando Rimachi; Delgado, Jorge Alcántara; Villasante, Yeny Aquino; Ortiz, Rodomiro.
Título: Detecting adventitious transgenic events in a maize center of diversity
Fonte: Electron. j. biotechnol;14(4):9-9, July 2011. ilus, tab.
Idioma: en.
Resumo: Background: The genetic diversity of maize in Peru includes several landraces (within race clusters) and modern open pollinated and hybrid cultivars that are grown by farmers across various regions, thereby making this country a secondary center of diversity for this crop. A main topic of controversy in recent years refers to the unintended presence of transgenic events in locally grown cultivars at main centers of crop diversity. Peru does not yet have biosafety regulations to control or permit the growing of genetically modified crops. Hence, the aim of this research was to undertake a survey in the valley of Barranca, where there were recent claims of authorized transgenic maize grown in farmers fields as well as in samples taken from feed storage and grain or seed trade centers. Results: A total of 162 maize samples (134 from fields, 15 from local markets, eight from the collecting centers of poultry companies, from the local trading center and four samples from seed markets) were included for a qualitative detection by the polymerase chain reaction (PCR) of Cauliflower Mosaic Virus (CaMV) 35S promoter (P35S) and nopaline synthase terminator (Tnos) sequences, as well as for six transgenic events, namely BT11, NK603, T25, 176, TC1507 and MON810. The 134 maize samples from farmers fields were negative for Cry1Ab delta-endotoxin insecticidal protein and enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) using lateral flow strips. The PCR analysis did not detect any of the six transgenic events in samples from farmers fields, local markets, seed trading shops and the local collecting center. There were four transgenic events (T25, NK603, MON810 and TC1507) in grain samples from the barns of poultry companies. Conclusions: This research could not detect, at the 95 percent probability level, transgenes in farmers' fields in the valley of Barranca. The four transgenic events in grain samples from barns of poultry companies...
Descritores: Variação Genética
Segurança
Transgenes
Zea mays/genética
-Alimentos Geneticamente Modificados
Peru
Responsável: CL1.1 - Biblioteca Central


  7 / 21 LILACS  
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Id: lil-608618
Autor: Amaral, Marta G; Campos, Vinicius F; Seixas, Fabiana K; Cavalcanti, Paulo V; Selau, Lisiane P. R; Deschamps, Joao C; Collares, Tiago.
Título: Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
Fonte: Biol. Res;44(3):229-234, 2011. ilus, tab.
Idioma: en.
Projeto: FAPERGS/Edital PROADE III; . CNPq.
Resumo: Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or liposome (Lipofectin) in an attempt to improve TMGT. Males receiving consecutive DNA complex injections were mated to normal females to obtain F0 progeny. In vivo evaluation of EGFP expression, RT-PCR and PCR were used to detect the expression and the presence of exogenous DNA in the progeny. We also evaluated possible testicular damage by histological procedures. PC R and RT-PCR analyses revealed that liposome and DMSO increased the rate of TMGT. Histological analyses demonstrated that repeated (4 times) injections of DNA complexes can affect spermatogenesis. DMSO was the most deleterious among the reagents tested. In this study, we detected the presence of transgene in the progeny, and its expression in blood cells. Consecutive injections of DNA complexes were associated with impaired spermatogenesis, suggesting requirement of optimal conditions for DNA delivery through TMGT.
Descritores: Dimetil Sulfóxido/farmacologia
Técnicas de Transferência de Genes
Proteínas de Fluorescência Verde/administração & dosagem
Camundongos Transgênicos/genética
Testículo
Transgenes
-Animais Geneticamente Modificados
Vetores Genéticos/administração & dosagem
Vetores Genéticos/genética
Proteínas de Fluorescência Verde/análise
Proteínas de Fluorescência Verde/genética
Lipossomos/farmacologia
Camundongos Endogâmicos BALB C
Reação em Cadeia da Polimerase
Testículo/efeitos dos fármacos
Testículo/patologia
Transfecção/métodos
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  8 / 21 LILACS  
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Id: lil-538014
Autor: Felmer, Ricardo; Arias, María Elena.
Título: Advances in the development of a noninvasive embryo model for the evaluation of the quality of cloned embryos subjected to different treatments
Fonte: Electron. j. biotechnol;11(5):2-3, Dec. 2008. ilus, tab.
Idioma: en.
Projeto: FONDEF; . FONDECYT.
Resumo: Total number of cells in cloned embryos is generally lower than that of in vivo derived embryos and in bovines cell allocation at the blastocyst stage, has been observed to be affected in a large proportion of cloned embryos. The current embryo staining procedures are toxic for mammalian cells and thus can not be used to determine the developmental potential of a stained embryo. Therefore, in the present study we sought to assess the feasibility to develop a noninvasive embryo model that would be suitable for the evaluation of cloned embryos subjected to different nuclear transfer and embryo culture procedures. For doing this, we stably transfected a bovine embryonic fibroblast cell line and generated a number of clones that constitutively expressed a red fluorescent protein (HcRed) in the nuclear compartment of the cell. Those clones with normal chromosomal content were further used as nuclear donor in nuclear transfer procedures (SCNT) to generate transgenic cloned embryos. These embryos expressed the red fluorescent protein in each blastomere, allowing their in vivo evaluation during development, thus demonstrating the potential of this model as a noninvasive tool for the assessment of the quality of cloned embryos.
Descritores: Clonagem de Organismos/métodos
Clonagem de Organismos
Transferência Embrionária
-Fibroblastos
Corantes Fluorescentes
Marcadores Genéticos
Técnicas Genéticas
Transgenes
Limites: Animais
Bovinos
Responsável: CL1.1 - Biblioteca Central


  9 / 21 LILACS  
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Id: lil-517020
Autor: Epstein, Alberto Luis.
Título: HSV-1-derived amplicon vectors: recent technological improvements and remaining difficulties: a review
Fonte: Mem. Inst. Oswaldo Cruz;104(3):399-410, May 2009. ilus.
Idioma: en.
Projeto: European Commission.
Resumo: Amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. As the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. In addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 Kbp of foreign DNA, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. We present here recent technological developments that have significantly improved and extended the use of amplicons, both in cultured cells and in living organisms. In addition, this review also discusses the many difficulties still pending to be solved, in order to achieve stable and physiologically regulated transgene expression.
Descritores: Técnicas de Transferência de Genes
Vetores Genéticos/genética
Herpesvirus Humano 1/genética
Transgenes/genética
-Engenharia Genética
Herpesvirus Humano 1/fisiologia
Limites: Animais
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


  10 / 21 LILACS  
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Id: lil-500934
Autor: Wilke, André Barreto Bruno.
Título: Controle genético de mosquitos Culex quinquefasciatus / Genetic control of Culex quinquefasciatus mosquitoes.
Fonte: São Paulo; s.n; 2008. 106 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia para obtenção do grau de Mestre.
Resumo: O mosquito Culex quinquefasciatus é considerado praga urbana e tem a capacidade de se desenvolver em águas altamente poluídas atingindo elevada densidade . Mosquitos desta espécie possuem importância vetorial na transmissão de parasitas e arboviroses. Medidas de controle químico têm se mostrado ineficazes, além de serem altamente prejudiciais ao meio ambiente. Desse modo, novas tecnologias de controle foram desenvolvidas, entre elas o SIT (Sterile Insect Technique) que utiliza radiação para esterilizar Machos e liberá-los no ambiente para copular com fêmeas selvagens. Posteriormente métodos genéticos baseados nessa técnica têm sido proposto. O sistema RIDL (Liberação de Insetos Carregando Gene Letal Dominante), consiste na integração de um gene letal dominante associado a um promotor específico de fêmea, dispensando a etapa de esterilização por radiação. Nesse processo os insetos recebem dieta suplementada com um repressor químico. A expressão do gene letal dominante é mantida desligada enquanto este repressor é adicionado ao meio das larvas. Para as amostras que estariam sendo preparadas para a liberação, o repressor é retirado, e o gene letal dominante é ativado, causando a morte de todas as fêmeas, restando apenas machos para liberação. Os machos homozigotos para gene letal seriam liberados para copular com fêmeas selvagens. A progênie seria heterozigota para o gene letal, porém semente os machos sobreviveriam. Parte crucial para o sucesso deste projeto foi à adaptação do método de microinjeção de embriões para a espécie Culex quinquefasciatus tornando possível à injeção dos transgenes LA513, LA882 e LA3653 com objetivo de obtermos linhagens transgênicas. A obtenção de linhagens transgênicas com estas construções se mostraram mais laboriosa do que o previsto, dificultando a transgenia. Porém, as aplicações práticas em controle de vetores utilizando a técnica do RIDL são imensas e pode se tornar uma importante ferramenta do Manejo Integrado de vetores.
Descritores: Animais Geneticamente Modificados
Culex/genética
Técnicas de Transferência de Genes
Transgenes
-Insetos Vetores
Controle de Vetores
Responsável: BR67.1 - CIR - Biblioteca - Centro de Informação e Referência
BR67.1; 595.771, 69. 49923/2008; BR67.1; MTR, 1581. CM. 49924/2008



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