Base de dados : LILACS
Pesquisa : G05.360.340.024.340.825 [Categoria DeCS]
Referências encontradas : 19 [refinar]
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  1 / 19 LILACS  
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Texto completo SciELO Brasil
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Id: lil-697834
Autor: Costa, Rodolfo; Stanewsky, Ralf.
Título: When population and evolutionary genetics met behaviour
Fonte: Mem. Inst. Oswaldo Cruz;108(supl.1):74-79, 2013. tab, graf.
Idioma: en.
Resumo: In this review, we analyse the impact of a population and evolutionary genetics approach on the study of insect behaviour. Our attention is focused on the model organism Drosophila melanogaster and several other insect species. In particular, we explore the relationship between rhythmic behaviours and the molecular evolution of clock and ion channel genes.
Descritores: Comportamento Animal/fisiologia
Relógios Circadianos/genética
Drosophila melanogaster/genética
Evolução Molecular
Genética Populacional
-Proteínas CLOCK/genética
Drosophila/genética
Especiação Genética
Canais Iônicos/genética
Proteínas Circadianas Period/genética
Psychodidae/genética
Comportamento Sexual Animal
Temperatura
Transgenes/genética
Limites: Animais
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  2 / 19 LILACS  
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Id: lil-695437
Autor: Pereyra-Bonnet, Federico.
Título: Plasticidad celular: convirtiendo una célula en otra / Cell plasticity: converting one cell into another
Fonte: Rev. Hosp. Ital. B. Aires (2004);32(4):188-190, dic. 2012. ilus.
Idioma: es.
Descritores: Fenômenos Fisiológicos Celulares
Células
Técnicas de Transferência de Genes
Células-Tronco
Transgenes
-Genética Médica/tendências
Organismos Geneticamente Modificados
Limites: Humanos
Responsável: AR2.1 - Biblioteca Central


  3 / 19 LILACS  
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Texto completo SciELO Chile
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Id: lil-646952
Autor: Hadi, Faranak; Salmanian, Ali Hatef; Ghazizadeh, Elham; Amani, Jafar; Noghabi, Kambiz Akbari; Mousavi, Amir.
Título: Development of quantitative competitive PCR for determination of copy number and expression level of the synthetic glyphosate oxidoreductase gene in transgenic canola plants
Fonte: Electron. j. biotechnol;15(4):2-2, July 2012. ilus, tab.
Idioma: en.
Resumo: Background: For successful in vitro plant regeneration, plant cell lines with multiple transgene integration and low transgene expression levels need to be ruled out. Although real-time polymerase chain reaction (RT-PCR) is a rapid way to accomplish this, it is also expensive and typically limits the size of the target sequence. Quantitative competitive PCR (QC-PCR) is proven to be a safe and accurate method for determination of both copy number and quantification of transcript levels of synthetic transgenes in transformed plants. Results: The glyphosate oxidoreductase genewas chemically synthesized and used to transform Brassica napus L. via Agrobactrium-mediated transformation. A construct containing the mutated form of a synthetic glyphosate oxidoreductase (gox) gene (internal standard) was prepared. Gene copy number was estimated in nine independent transgenic lines using QC-PCR as well as the standard method of Southern blot analysis. By quantitative RT-PCR, transcript levels were also determined in these lines. High (> 3), medium to high (2.2-3), medium to low (1-2.2), and low (< 1) levels of transcript were detected. Conclusions: No direct relationship was found between copy number and transgene expression levels. QC-PCR method could be implemented to screen putative transgenic plants and quickly select single T-DNA inserts. QC-PCR methods and the prepared competitor construct may be useful for future quantification of commercial transgenic food and feed.
Descritores: Brassica napus/enzimologia
Brassica napus/genética
Glicina/genética
Oxirredutases/genética
Reação em Cadeia da Polimerase/métodos
-Glicina/análogos & derivados
Glicina/fisiologia
Oxirredutases/fisiologia
Transcrição Genética
Transgenes
Responsável: CL1.1 - Biblioteca Central


  4 / 19 LILACS  
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Texto completo SciELO Chile
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Id: lil-640504
Autor: Gamarra, Luis Fernando Rimachi; Delgado, Jorge Alcántara; Villasante, Yeny Aquino; Ortiz, Rodomiro.
Título: Detecting adventitious transgenic events in a maize center of diversity
Fonte: Electron. j. biotechnol;14(4):9-9, July 2011. ilus, tab.
Idioma: en.
Resumo: Background: The genetic diversity of maize in Peru includes several landraces (within race clusters) and modern open pollinated and hybrid cultivars that are grown by farmers across various regions, thereby making this country a secondary center of diversity for this crop. A main topic of controversy in recent years refers to the unintended presence of transgenic events in locally grown cultivars at main centers of crop diversity. Peru does not yet have biosafety regulations to control or permit the growing of genetically modified crops. Hence, the aim of this research was to undertake a survey in the valley of Barranca, where there were recent claims of authorized transgenic maize grown in farmers fields as well as in samples taken from feed storage and grain or seed trade centers. Results: A total of 162 maize samples (134 from fields, 15 from local markets, eight from the collecting centers of poultry companies, from the local trading center and four samples from seed markets) were included for a qualitative detection by the polymerase chain reaction (PCR) of Cauliflower Mosaic Virus (CaMV) 35S promoter (P35S) and nopaline synthase terminator (Tnos) sequences, as well as for six transgenic events, namely BT11, NK603, T25, 176, TC1507 and MON810. The 134 maize samples from farmers fields were negative for Cry1Ab delta-endotoxin insecticidal protein and enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) using lateral flow strips. The PCR analysis did not detect any of the six transgenic events in samples from farmers fields, local markets, seed trading shops and the local collecting center. There were four transgenic events (T25, NK603, MON810 and TC1507) in grain samples from the barns of poultry companies. Conclusions: This research could not detect, at the 95 percent probability level, transgenes in farmers' fields in the valley of Barranca. The four transgenic events in grain samples from barns of poultry companies...
Descritores: Variação Genética
Segurança
Transgenes
Zea mays/genética
-Alimentos Geneticamente Modificados
Peru
Responsável: CL1.1 - Biblioteca Central


  5 / 19 LILACS  
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Texto completo SciELO Chile
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Id: lil-608618
Autor: Amaral, Marta G; Campos, Vinicius F; Seixas, Fabiana K; Cavalcanti, Paulo V; Selau, Lisiane P. R; Deschamps, Joao C; Collares, Tiago.
Título: Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
Fonte: Biol. Res;44(3):229-234, 2011. ilus, tab.
Idioma: en.
Projeto: FAPERGS/Edital PROADE III; . CNPq.
Resumo: Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or liposome (Lipofectin) in an attempt to improve TMGT. Males receiving consecutive DNA complex injections were mated to normal females to obtain F0 progeny. In vivo evaluation of EGFP expression, RT-PCR and PCR were used to detect the expression and the presence of exogenous DNA in the progeny. We also evaluated possible testicular damage by histological procedures. PC R and RT-PCR analyses revealed that liposome and DMSO increased the rate of TMGT. Histological analyses demonstrated that repeated (4 times) injections of DNA complexes can affect spermatogenesis. DMSO was the most deleterious among the reagents tested. In this study, we detected the presence of transgene in the progeny, and its expression in blood cells. Consecutive injections of DNA complexes were associated with impaired spermatogenesis, suggesting requirement of optimal conditions for DNA delivery through TMGT.
Descritores: Dimetil Sulfóxido/farmacologia
Técnicas de Transferência de Genes
Proteínas de Fluorescência Verde/administração & dosagem
Camundongos Transgênicos/genética
Testículo
Transgenes
-Animais Geneticamente Modificados
Vetores Genéticos/administração & dosagem
Vetores Genéticos/genética
Proteínas de Fluorescência Verde/análise
Proteínas de Fluorescência Verde/genética
Lipossomos/farmacologia
Camundongos Endogâmicos BALB C
Reação em Cadeia da Polimerase
Testículo/efeitos dos fármacos
Testículo/patologia
Transfecção/métodos
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 19 LILACS  
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Texto completo SciELO Chile
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Id: lil-538014
Autor: Felmer, Ricardo; Arias, María Elena.
Título: Advances in the development of a noninvasive embryo model for the evaluation of the quality of cloned embryos subjected to different treatments
Fonte: Electron. j. biotechnol;11(5):2-3, Dec. 2008. ilus, tab.
Idioma: en.
Projeto: FONDEF; . FONDECYT.
Resumo: Total number of cells in cloned embryos is generally lower than that of in vivo derived embryos and in bovines cell allocation at the blastocyst stage, has been observed to be affected in a large proportion of cloned embryos. The current embryo staining procedures are toxic for mammalian cells and thus can not be used to determine the developmental potential of a stained embryo. Therefore, in the present study we sought to assess the feasibility to develop a noninvasive embryo model that would be suitable for the evaluation of cloned embryos subjected to different nuclear transfer and embryo culture procedures. For doing this, we stably transfected a bovine embryonic fibroblast cell line and generated a number of clones that constitutively expressed a red fluorescent protein (HcRed) in the nuclear compartment of the cell. Those clones with normal chromosomal content were further used as nuclear donor in nuclear transfer procedures (SCNT) to generate transgenic cloned embryos. These embryos expressed the red fluorescent protein in each blastomere, allowing their in vivo evaluation during development, thus demonstrating the potential of this model as a noninvasive tool for the assessment of the quality of cloned embryos.
Descritores: Clonagem de Organismos/métodos
Clonagem de Organismos
Transferência Embrionária
-Fibroblastos
Corantes Fluorescentes
Marcadores Genéticos
Técnicas Genéticas
Transgenes
Limites: Animais
Bovinos
Responsável: CL1.1 - Biblioteca Central


  7 / 19 LILACS  
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Id: lil-517020
Autor: Epstein, Alberto Luis.
Título: HSV-1-derived amplicon vectors: recent technological improvements and remaining difficulties: a review
Fonte: Mem. Inst. Oswaldo Cruz;104(3):399-410, May 2009. ilus.
Idioma: en.
Projeto: European Commission.
Resumo: Amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. As the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. In addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 Kbp of foreign DNA, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. We present here recent technological developments that have significantly improved and extended the use of amplicons, both in cultured cells and in living organisms. In addition, this review also discusses the many difficulties still pending to be solved, in order to achieve stable and physiologically regulated transgene expression.
Descritores: Técnicas de Transferência de Genes
Vetores Genéticos/genética
Herpesvirus Humano 1/genética
Transgenes/genética
-Engenharia Genética
Herpesvirus Humano 1/fisiologia
Limites: Animais
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


  8 / 19 LILACS  
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Id: lil-500934
Autor: Wilke, André Barreto Bruno.
Título: Controle genético de mosquitos Culex quinquefasciatus / Genetic control of Culex quinquefasciatus mosquitoes.
Fonte: São Paulo; s.n; 2008. 106 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia para obtenção do grau de Mestre.
Resumo: O mosquito Culex quinquefasciatus é considerado praga urbana e tem a capacidade de se desenvolver em águas altamente poluídas atingindo elevada densidade . Mosquitos desta espécie possuem importância vetorial na transmissão de parasitas e arboviroses. Medidas de controle químico têm se mostrado ineficazes, além de serem altamente prejudiciais ao meio ambiente. Desse modo, novas tecnologias de controle foram desenvolvidas, entre elas o SIT (Sterile Insect Technique) que utiliza radiação para esterilizar Machos e liberá-los no ambiente para copular com fêmeas selvagens. Posteriormente métodos genéticos baseados nessa técnica têm sido proposto. O sistema RIDL (Liberação de Insetos Carregando Gene Letal Dominante), consiste na integração de um gene letal dominante associado a um promotor específico de fêmea, dispensando a etapa de esterilização por radiação. Nesse processo os insetos recebem dieta suplementada com um repressor químico. A expressão do gene letal dominante é mantida desligada enquanto este repressor é adicionado ao meio das larvas. Para as amostras que estariam sendo preparadas para a liberação, o repressor é retirado, e o gene letal dominante é ativado, causando a morte de todas as fêmeas, restando apenas machos para liberação. Os machos homozigotos para gene letal seriam liberados para copular com fêmeas selvagens. A progênie seria heterozigota para o gene letal, porém semente os machos sobreviveriam. Parte crucial para o sucesso deste projeto foi à adaptação do método de microinjeção de embriões para a espécie Culex quinquefasciatus tornando possível à injeção dos transgenes LA513, LA882 e LA3653 com objetivo de obtermos linhagens transgênicas. A obtenção de linhagens transgênicas com estas construções se mostraram mais laboriosa do que o previsto, dificultando a transgenia. Porém, as aplicações práticas em controle de vetores utilizando a técnica do RIDL são imensas e pode se tornar uma importante ferramenta do Manejo Integrado de vetores.
Descritores: Animais Geneticamente Modificados
Culex/genética
Técnicas de Transferência de Genes
Transgenes
-Insetos Vetores
Controle de Vetores
Responsável: BR67.1 - CIR - Biblioteca - Centro de Informação e Referência
BR67.1; 595.771, 69. 49923/2008; BR67.1; MTR, 1581. CM. 49924/2008


  9 / 19 LILACS  
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Id: lil-497553
Autor: Pereira, Lygia da Veiga.
Título: Animais transgênicos: nova fronteira do saber / Transgenic animals: new frontier of knowledge
Fonte: Ciênc. cult. (Säo Paulo);60(2):40-42, abr.-jun. 2008.
Idioma: pt.
Descritores: Animais Geneticamente Modificados
Camundongos/genética
Transgenes
Limites: Animais
Responsável: BR513.1 - Biblioteca Central


  10 / 19 LILACS  
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Id: lil-482025
Autor: Abud, S; Souza, P. I. de; Vianna, G. R; Leonardecz, E; Moreira, C. T; Faleiro, F. G; N. Júnior, J; Monteiro, P. M; Rech, E. L; Aragão, F. J.
Título: Gene flow from transgenic to nontransgenic soybean plants in the Cerrado region of Brazil
Fonte: Genet. mol. res. (Online);6(2):445-452, 2007. ilus, tab, graf.
Idioma: en.
Resumo: Evaluation of transgenic crops under field conditions is a fundamental step for the production of genetically engineered varieties. In order to determine if there is pollen dispersal from transgenic to nontransgenic soybean plants, a field release experiment was conducted in the Cerrado region of Brazil. Nontransgenic plants were cultivated in plots surrounding Roundup Ready transgenic plants carrying the cp4 epsps gene, which confers herbicide tolerance against glyphosate herbicide, and pollen dispersal was evaluated by checking for the dominant gene. The percentage of cross-pollination was calculated as a fraction of herbicide-tolerant and -nontolerant plants. The greatest amount of transgenic pollen dispersion was observed in the first row, located at one meter from the central (transgenic) plot, with a 0.52% average frequency. The frequency of pollen dispersion decreased to 0.12% in row 2, reaching 0% when the plants were up to 10 m distance from the central plot. Under these conditions pollen flow was higher for a short distance. This fact suggests that the management necessary to avoid cross-pollination from transgenic to nontransgenic plants in the seed production fields should be similar to the procedures currently utilized to produce commercial seeds.
Descritores: Soja/genética
Fluxo Gênico
Plantas Geneticamente Modificadas/genética
-Brasil
Cruzamentos Genéticos
Genes Dominantes
Genes de Plantas
Engenharia Genética
Modelos Genéticos
Plantas/genética
Pólen/metabolismo
Análise de Regressão
Sementes/metabolismo
Transgenes
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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