Base de dados : LILACS
Pesquisa : G05.360.340.358.365 [Categoria DeCS]
Referências encontradas : 12 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2 ir para página        

  1 / 12 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Saúde Pública
Celeste, Roger Keller
Texto completo
Id: lil-736429
Autor: Teixeira, Maurício Fernando Nunes; Martins, Aline Blaya; Celeste, Roger Keller; Hugo, Fernando Neves; Hilgert, Juliana Balbinot.
Título: Associação entre resiliência e qualidade de vida relacionada à saúde bucal em idosos / Association between resilience and quality of life related to oral health in the elderly
Fonte: Rev. bras. epidemiol;18(1):220-233, Jan-Mar/2015. tab, graf.
Idioma: pt.
Resumo: OBJETIVO: Avaliar a associação entre resiliência e qualidade de vida relacionada à saúde bucal, por meio de uma abordagem hierárquica baseada em um modelo teórico conceitual em uma coorte de idosos do Rio Grande do Sul. MÉTODOS: Foi conduzido um estudo transversal aninhado a um estudo de coorte, em 2008. Foram avaliados 498 idosos de Carlos Barbosa, Rio Grande do Sul. As medidas avaliadas foram sociodemográficas, comportamentos de saúde, qualidade de vida relacionada à saúde bucal, medida pelo Oral Health Impact Profile (OHIP-14), Escala de Resiliência e CPOD. A associação entre o potencial de resiliência e os impactos na percepção de saúde bucal relacionados à qualidade de vida foi verificada por meio de regressão binomial negativa. Razões das médias (RM) são apresentadas com seus intervalos de confiança de 95% (IC95%). RESULTADOS: Maiores médias do OHIP foram encontradas entre mulheres (6,7 ± 6,3; p = 0,011), moradores da zona rural (7,3 ± 6,7; p = 0,004) e solteiros (8,0 ± 6,3; p = 0,032). O modelo final da análise multivariada mostrou que ser morador da zona rural (RM = 1,32; IC95% 1,06 - 1,65) e casado (RM = 1,36; IC95% 1,07 - 1,72) foram variáveis independentemente associadas à qualidade de vida relacionada à saúde bucal. Não houve associação entre resiliência e qualidade de vida relacionada à saúde bucal. CONCLUSÃO: Os resultados sugerem que variáveis sociodemográficas estão associados à qualidade de vida relacionada à saúde bucal. A hipótese de que a resiliência pudesse exercer um papel importante no desfecho não foi confirmada. .

OBJECTIVE: To evaluate the association between psychological resilience and oral health related to quality of life through a hierarchical approach based on a conceptual theoretical model in a cohort of elderly residents in Rio Grande do Sul, Brazil. METHODS: We conducted a cross-sectional study nested in a cohort study in 2008. We evaluated 498 elderly residents in Carlos Barbosa, Rio Grande do Sul. The measures included sociodemographic questionnaire, health behavior, quality of life related to oral health (OHRQOL), measured by the Oral Health Impact Profile (OHIP-14), Resilience Scale, and DMFT. The association between resilience and potential impacts on perceptions of oral health-related quality of life was assessed through negative binomial regression. Mean ratios (MR) are presented with 95% confidence intervals (95%CI). RESULTS: Higher means of OHIP were found in women 6.7 ± 6.3; p = 0.011), in rural dwellers (7.3 ± 6.7; p = 0.004) and singles (8.0 ± 6.3; p = 0.032). The final model of multivariate analysis showed that being a rural dweller (MR = 1.32; 95%CI 1.06 - 1.65) and being married (MR = 1.36; 95%CI 1.07 - 1.72) were independently associated with OHRQOL. There was no association between resilience and OHRQOL. CONCLUSION: The results suggest that factors such as sociodemographic variables are associated with OHRQOL. The hypothesis that resilience might play a role in the outcome has not been confirmed. .
Descritores: Fusarium/genética
Genoma Fúngico
Polimorfismo Genético
-DNA Fúngico
Evolução Molecular
Fusarium/fisiologia
Hordeum/microbiologia
Dados de Sequência Molecular
Mutação Puntual
Polimorfismo de Nucleotídeo Único
Doenças das Plantas/microbiologia
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME


  2 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-634527
Autor: Grasso, Daniel.
Título: Metagenómica: un viaje a las estrellas
Fonte: Rev. argent. microbiol;38(4):189-189, oct.-dic. 2006.
Idioma: es.
Descritores: Genômica/tendências
-Ecossistema
Genoma Arqueal/genética
Genoma Bacteriano/genética
Genoma Fúngico/genética
Genômica/organização & administração
Tipo de Publ: Editorial
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


  3 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-531924
Autor: Islam, Mohammed M.
Título: Molecular cloning, expression and characterization of a serine proteinase from Japanese edible mushroom, Grifola frondosa: solving the structure - function anomaly of a reported aminopeptidase
Fonte: Electron. j. biotechnol;11(4):10-11, Oct. 2008. ilus, tab.
Idioma: en.
Resumo: The N-terminal amino acid sequence of an aminopeptidase from Japanese edible mushroom, Grifola frondosa, was reported to have high similarity with that of a serine proteinase from basidiomycete, Agaricus bisporous (Nishiwaki and Hayashi, 2001). The full-length cDNA and the corresponding genomic DNA of the enzyme were cloned, based on the reported N-terminal amino acid sequence. The predicted open reading frame (ORF) of the cloned cDNA, encoding a product of 379 amino acids, was expressed in E. coli using pET expression vector. The expressed pro-enzyme (40 kDa) underwent autolysis to produce the mature protein (30 kDa) and a pro-peptide (10 kDa). The mature protein and the pro-peptide remained tightly bound to each other and could not be separated by Ni-NTA metal affinity chromatography or Q-Sepharose ion-exchange chromatography. The enzyme was inactive in the bound form. Upon treatment with subtilisin, the bound pro-peptide was further hydrolyzed and a high serine proteinase activity was recovered. No aminopeptidase activity was detected at any stage of the protein processing. These results clearly indicated that the N-terminal amino acid sequence and the function of the reported aminopeptidase were not derived from the same protein entity and hence caused the structure-function anomaly.
Descritores: Aminopeptidases
Agaricus/enzimologia
Agaricus/genética
Clonagem Molecular
Grifola/enzimologia
Grifola/genética
-Análise de Sequência de Proteína/métodos
DNA Complementar
Genoma Fúngico/genética
Reação em Cadeia da Polimerase
Responsável: CL1.1 - Biblioteca Central


  4 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-482073
Autor: Melo, S. C; Pungartnik, C; Cascardo, J. C; Brendel, M.
Título: Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa
Fonte: Genet. mol. res. (Online);5(4):851-855, 2006. ilus.
Idioma: en.
Resumo: DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yield from the hyphae of Crinipellis perniciosa, which causes witches' broom disease in cacao, from three other filamentous fungi, Lentinus edodes, Agaricus blazei, Trichoderma stromaticum, and from the yeast S. cerevisiae. Genomic DNA was suitable for PCR of specific actin primers of C. perniciosa, allowing it to be differentiated from fungal contaminants, including its natural competitor, T. stromaticum.
Descritores: Agaricales/genética
DNA Fúngico/isolamento & purificação
Genoma Fúngico/genética
Técnicas de Tipagem Micológica/métodos
-Agaricales/classificação
DNA Fúngico/genética
Eletroforese em Gel de Ágar
Reação em Cadeia da Polimerase
Reprodutibilidade dos Testes
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-482029
Autor: Ferreira, R. C; Bosco, F; Paiva, P. B; Briones, M. R.
Título: Minimization of transcriptional temporal noise and scale invariance in the yeast genome
Fonte: Genet. mol. res. (Online);6(2):397-414, 2007. graf, tab.
Idioma: en.
Resumo: The analysis of transcriptional temporal noise could be an interesting means to study gene expression dynamics and stochasticity in eukaryotes. To study the statistical distributions of temporal noise in the eukaryotic model system Saccharomyces cerevisiae, we analyzed microarray data corresponding to one cell cycle for 6200 genes. We found that the temporal noise follows a lognormal distribution with scale invariance at the genome, chromosomal and sub-chromosomal levels. Correlation of temporal noise with the codon adaptation index suggests that at least 70% of all protein-coding genes are a noise minimization core of the genome. Accordingly, a mathematical model of individual gene expression dynamics was proposed, using an operator theoretical approach, which reveals strict conditions for noise variability and a possible global noise minimization/optimization strategy at the genome level. Our model and data show that minimal noise does not correspond to genes obeying a strictly deterministic dynamics. The natural strategy of minimization consists in equating the mean of the absolute value of the relative variation of the expression level (alpha) with noise (eta). We hypothesize that the temporal noise pattern is an emergent property of the genome and shows how the dynamics of gene expression could be related to chromosomal organization.
Descritores: Genoma Fúngico
Saccharomyces cerevisiae/genética
Transcrição Genética
-Regulação Fúngica da Expressão Gênica
Redes Reguladoras de Genes
Modelos Genéticos
Modelos Estatísticos
Modelos Teóricos
Análise de Sequência com Séries de Oligonucleotídeos
Saccharomyces cerevisiae/metabolismo
Fatores de Tempo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Azevedo, Joäo Lucio de
Texto completo
Id: lil-450994
Autor: Fávaro, Léia Cecilia de Lima; Araújo, Welington Luiz de; Azevedo, João Lúcio de; Paccola-Meirelles, Luzia Doretto.
Título: The biology and potential for genetic research of transposable elements in filamentous fungi
Fonte: Genet. mol. biol;28(4):804-813, Dec. 2005. ilus, tab.
Idioma: en.
Resumo: Recently many transposable elements have been identified and characterized in filamentous fungi, especially in species of agricultural, biotechnological and medical interest. Similar to the elements found in other eukaryotes, fungal transposons can be classified as class I elements (retrotransposons) that use RNA and reverse transcriptase and class II elements (DNA transposons) that use DNA. The changes (transposition and recombination) caused by transposons can supply wide-ranging genetic variation, especially for species that do not have a sexual phase. The application of transposable elements to gene isolation and population analysis is an important tool for molecular biology and studies of fungal evolution
Descritores: DNA Fúngico
Fungos/genética
Genoma Fúngico/genética
-Elementos de DNA Transponíveis
Limites: Animais
Responsável: BR26.1 - Biblioteca Central


  7 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Brígido, M. M
Texto completo
Id: lil-445291
Autor: Brígido, M. M; Walter, M. E; Oliveira, A. G; Inoue, M. K; Anjos, D. S; Sandes, E. F; Gondim, J. J; Carvalho, M. J; Almeida Jr, N. F; Felipe, M. S.
Título: Bioinformatics of the Paracoccidioides brasiliensis EST Project
Fonte: Genet. mol. res. (Online);4(2):203-215, 30 jun. 2005. ilus, graf, tab.
Idioma: en.
Resumo: Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an endemic mycosis of Latin America. This fungus presents a dimorphic character; it grows as a mycelium at room temperature, but it is isolated as yeast from infected individuals. It is believed that the transition from mycelium to yeast is important for the infective process. The Functional and Differential Genome of Paracoccidioides brasiliensis Project--PbGenome Project was developed to study the infection process by analyzing expressed sequence tags--ESTs, isolated from both mycelial and yeast forms. The PbGenome Project was executed by a consortium that included 70 researchers (professors and students) from two sequencing laboratories of the midwest region of Brazil; this project produced 25,741 ESTs, 19,718 of which with sufficient quality to be analyzed. We describe the computational procedures used to receive process, analyze these ESTs, and help with their functional annotations; we also detail the services that were used for sequence data exploration. Various programs were compared for filtering and grouping the sequences, and they were adapted to a user-friendly interface. This system made the analysis of the differential transcriptome of P. brasiliensis possible.
Descritores: Biologia Computacional/métodos
Etiquetas de Sequências Expressas
Genoma Fúngico/genética
Paracoccidioides/genética
Transcrição Genética/genética
-Brasil
Regulação Fúngica da Expressão Gênica/genética
Interface Usuário-Computador
Responsável: BR1.1 - BIREME


  8 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-445288
Autor: Albuquerque, P; Baptista, A. J; Derengowsky, L. da S; Procópio, L; Nicola, A. M; Arraes, F. B; Souza, D. P; Kyaw, C. M; Silva-Pereira, I.
Título: Paracoccidioides brasiliensis RNA biogenesis apparatus revealed by functional genome analysis
Fonte: Genet. mol. res. (Online);4(2):251-272, 30 jun. 2005. tab.
Idioma: en.
Resumo: The RNA biogenesis machinery of Paracoccidioides brasiliensis was assessed by comparative analyses of PbAESTs (P. brasiliensis assembled expressed sequence tags (ESTs)) with sequences from Saccharomyces cerevisiae MIPS database. PbAESTs related to almost all categories of S. cerevisiae RNA biogenesis were found. Two of the 12 S. cerevisiae RNA Pol II core subunits, Rpb3 and Rpb7, were found, probably reflecting the growth phase from which the cDNA libraries used in ESTs generation were constructed, as well as the low abundance of some of these transcripts. We have also found orthologs to TATA-box-binding protein (TBP), and at least one subunit of each TBP-associated factors (TFII) in P. brasiliensis transcriptome, except TFIIB. Genes associated to the chromatin remodeling complex, as well as transcription factors probably involved in the control of genes associated to a sexual cycle and virulence, were also identified. With respect to the pre-mRNA processing, 65 PbAEST orthologs to S. cerevisiae basal splicing machinery and 21 orthologs of 5'- and 3'-end formation processes were found. Components involved in RNA interference were detected, suggesting that this gene expression regulation mechanism is probably used by P. brasiliensis. Twelve PbAESTs related to Pol I and Pol III machineries were assigned as S. cerevisiae orthologs. Finally, 25 and 10 PbAESTs associated to rRNA and tRNA processing, respectively, were detected. Taken together, our results enable us to depict, for the first time, a global view of transcription and RNA processing in P. brasiliensis.
Descritores: Origem da Vida
Etiquetas de Sequências Expressas
Fatores de Transcrição/genética
Paracoccidioides/genética
-Fatores de Transcrição/fisiologia
Genoma Fúngico
Paracoccidioides/fisiologia
Reprodução
RNA Fúngico/genética
RNA Polimerase II/genética
RNA Polimerase II/fisiologia
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/fisiologia
Transcrição Genética/fisiologia
Limites: Humanos
Responsável: BR1.1 - BIREME


  9 / 12 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-445287
Autor: Souza, D. P; Silva, S. S; Baptista, A. J; Nicola, A. M; Kyaw, C. M; Silva-Pereira, I.
Título: Paracoccidioides brasiliensis translation and protein fate machineries revealed by functional genome analysis
Fonte: Genet. mol. res. (Online);4(2):273-289, 30 jun. 2005. tab.
Idioma: en.
Resumo: The translational and post-translational modification machineries of Paracoccidioides brasiliensis were assessed by means of comparative analyses of PbAESTs (P. brasiliensis assembled expressed sequence tags) with sequences deposited on different databases. Of the 79 sequences corresponding to cytosolic ribosomal proteins, we were able to find 78 in the P. brasiliensis transcriptome. Nineteen of the 27 Saccharomyces cerevisiae genes related to translation initiation were also found. All eukaryotic elongation factors were detected in P. brasiliensis transcriptome, with eEF1A as one of the most expressed genes. Translation termination is performed, in eukaryotes, by factors 1 and 3 (eRF1, eRF3). In P. brasiliensis transcriptome it was possible to identify eRF3, but not eRF1. Sixteen PbAESTs showing aminoacyl-tRNA synthetase-predicted activities were found in our analyses, but no cysteinyl-, leucyl-, asparagyl- and arginyl-tRNA synthetases were detected. Among the mitochondrial ribosomal proteins, we have found 20 and 18 orthologs to S. cerevisiae large and small ribosomal subunit proteins, respectively. We have also found three PbAESTs similar to Neurospora crassa mitochondrial ribosomal genes, with no similarity with S. cerevisiae genes. Although orthologs to S. cerevisiae mitochondrial EF-Tu, EF-G and RF1 have been found in P. brasiliensis transcriptome, no sequences corresponding to functional EF-Ts were detected. In addition, 64 and 28 PbAESTs associated to protein modification and degradation, respectively, were found. These results suggest that these machineries are well conserved in P. brasiliensis, when compared to other organisms.
Descritores: Genoma Fúngico/genética
Modificação Traducional de Proteínas/genética
Paracoccidioides/metabolismo
Proteínas Ribossômicas/metabolismo
-Etiquetas de Sequências Expressas/metabolismo
Regulação da Expressão Gênica
Paracoccidioides/genética
Proteínas Ribossômicas/genética
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Transcrição Genética
Responsável: BR1.1 - BIREME


  10 / 12 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-410889
Autor: Lopes, Francis J F; Araújo, Elza F de; Queiroz, Marisa V de.
Título: Easy detection of green fluorescent protein multicopy transformants in Penicillium griseoroseum
Fonte: Genet. mol. res. (Online);3(4):449-455, 2004. ilus, tab.
Idioma: en.
Resumo: Penicillium griseoroseum, a deuteromycete fungus producer of pectinolytic enzymes, was transformed with a gene encoding for green fluorescent protein (GFP). The selection of transformants was based on the homologous nitrate reductase gene (niaD). Protoplasts of a P. griseoroseum Nia mutant (PG63) were co-transformed with the plasmids pNPG1 and pAN52-1-GFP. The plasmid pNPG-1 carries the homologous niaD gene and pAN52-1-GFP carries the SGFP-TYG version of GFP. The highest transformation efficiency (102 transformants/µg of pNPG1) resulted from the utilization of equimolar amounts of transforming and co-transforming vectors. Analysis of pAN52-1-GFP insertions into the genomic DNA of the transformants revealed single and multiple copy integrations. The transformants possessing a single copy of the gfp gene showed a low level of fluorescence, whereas multicopy transformants displayed strong fluorescence under visualization with fluorescent light. The transformants showing high expression of the gfp gene had the normal mycelia pigmentation altered, displaying a bright green-yellowish color, visible with the naked eye on the plates, without the aid of any kind of fluorescent light or special filter set.
Descritores: DNA Fúngico/genética
Genoma Fúngico
Proteínas Luminescentes/genética
Mutação
Penicillium/genética
Transformação Genética/genética
-Proteínas Luminescentes/análise
Microscopia de Fluorescência
Penicillium/enzimologia
Plasmídeos/genética
Poligalacturonase/genética
Protoplastos/enzimologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



página 1 de 2 ir para página        
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde