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Referências encontradas : 35 [refinar]
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Id: biblio-1051590
Autor: Bi, Rabiya; Chandappa, Lohithaswa H; Siddalingaiah, Lokesh; Kenchanmane Raju, Sunil Kumar; Hassan Balakrishna, Shilpa; Kumar, Jyothi; Kuruba, Vinutha; Hittalmani, Shailaja.
Título: Leveraging barrel medic genome sequence for the development and use of genomic resources for genetic analysis and breeding in legumes
Fonte: Electron. j. biotechnol;39:30-41, may. 2019. tab, ilus.
Idioma: en.
Projeto: Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi, India.
Resumo: BACKGROUND: A total of 62,591 cowpea expressed sequence tags (ESTs) were BLAST aligned to the whole-genome sequence of barrel medic (Medicago truncatula) to develop conserved intron scanning primers (CISPs). The efficacy of the primers was tested across 10 different legumes and on different varieties of cowpea, chickpea, and pigeon pea. Genetic diversity was assessed using the same primers on different cowpea genotypes. Singlenucleotide polymorphisms (SNPs) were detected, which were later converted to length polymorphism markers for easy genotyping. CISPs developed in this study were used in tagging resistance to bacterial leaf blight disease in cowpea. RESULTS: A total of 1262 CISPs were designed. The single-copy amplification success rates using these primers on 10 different legumes and on different varieties of cowpea, chickpea, and pigeon pea were approximately 60% in most of the legumes except soybean (47%) and peanut (37%). Genetic diversity analysis of 35 cowpea genotypes using 179 CISPs revealed 123 polymorphic markers with PIC values ranging from 0.05 to 0.59. Potential SNPs identified in cowpea, chickpea, and pigeon pea were converted to PCR primers of various sizes for easy genotyping. Using the markers developed in this study, a genetic linkage map was constructed with 11 linkage groups in cowpea. QTL mapping with 194 F3 progeny families derived from the cross C-152 × V-16 resulted in the identification of three QTLs for resistance to bacterial leaf blight disease. Conclusions: CISPs were proved to be efficient markers to identify various other marker classes like SNPs through comparative genomic studies in lesser studied crops and to aid in systematic sampling of the entire genome for well-distributed markers at low cost
Descritores: Genoma de Planta
Genômica/métodos
Medicago truncatula/genética
-Reação em Cadeia da Polimerase
Mapeamento Cromossômico
Etiquetas de Sequências Expressas
Polimorfismo de Nucleotídeo Único
Genômica
Locos de Características Quantitativas
Fabaceae/genética
Responsável: CL1.1 - Biblioteca Central


  2 / 35 LILACS  
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Id: biblio-1124206
Autor: Li, Bin; Lin, Furong; Huang, Ping; Guo, Wenying; Zheng, Yongqi.
Título: Development of nuclear SSR and chloroplast genome markers in diverse Liriodendron chinense germplasm based on low-coverage whole genome sequencing
Fonte: Biol. Res;53:21, 2020. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Forest and Grass Germplasm Resources Bank Program.
Resumo: BACKGROUND: Liriodendron chinense ranges widely in subtropical China and northern Vietnam; however, it inhabits several small, isolated populations and is now an endangered species due to its limited seed production. The objective of this study was to develop a set of nuclear SSR (simple sequence repeats) and multiple chloroplast genome markers for genetic studies in L. chinense and their characterization in diverse germplasm. RESULTS: We performed low-coverage whole genome sequencing of the L. chinense from four genotypes, assembled the chloroplast genome and identified nuclear SSR loci by searching in contigs for SSR motifs. Comparative analysis of the four chloroplast genomes of L. chinense revealed 45 SNPs, 17 indels, 49 polymorphic SSR loci, and five small inversions. Most chloroplast intraspecific polymorphisms were located in the interspaces of single-copy regions. In total, 6147 SSR markers were isolated from low-coverage whole genome sequences. The most common SSR motifs were dinucleotide (70.09%), followed by trinucleotide motifs (23.10%). The motif AG/TC (33.51%) was the most abundant, followed by TC/AG (25.53%). A set of 13 SSR primer combinations were tested for amplification and their ability to detect polymorphisms in a set of 109 L. chinense individuals, representing distinct varieties or germplasm. The number of alleles per locus ranged from 8 to 28 with an average of 21 alleles. The expected heterozygosity (He) varied from 0.19 to 0.93 and the observed heterozygosity (Ho) ranged from 0.11 to 0.79. CONCLUSIONS: The genetic resources characterized and tested in this study provide a valuable tool to detect polymorphisms in L. chinense for future genetic studies and breeding programs.
Descritores: Polimorfismo Genético/genética
Genoma de Planta/genética
Liriodendron/genética
Genoma de Cloroplastos/genética
-Primers do DNA/genética
DNA de Plantas/genética
Repetições de Microssatélites
Alelos
Sequenciamento Completo do Genoma
Genótipo
Responsável: CL1.1 - Biblioteca Central


  3 / 35 LILACS  
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Id: biblio-1124208
Autor: Li, Huawei; Guan, Haiying; Zhuo, Qicui; Wang, Zongshuai; Li, Shengdong; Si, Jisheng; Zhang, Bin; Feng, Bo; Kong, Ling-an; Wang, Fahong; Wang, Zheng; Zhang, Lishun.
Título: Genome-wide characterization of the abscisic acid-, stress- and ripening-induced (ASR) gene family in wheat (Triticum aestivum L)
Fonte: Biol. Res;53:23, 2020. tab, graf.
Idioma: en.
Projeto: National Key Research and Development Program of China; . National Natural Science Foundation of China; . Taishan Industry.
Resumo: BACKGROUND: Abscisic acid-, stress-, and ripening-induced (ASR) genes are a class of plant specific transcription factors (TFs), which play important roles in plant development, growth and abiotic stress responses. The wheat ASRs have not been described in genome-wide yet. METHODS: We predicted the transmembrane regions and subcellular localization using the TMHMM server, and Plant-mPLoc server and CELLO v2.5, respectively. Then the phylogeny tree was built by MEGA7. The exon-intron structures, conserved motifs and TFs binding sites were analyzed by GSDS, MEME program and PlantRegMap, respectively. RESULTS: In wheat, 33ASR genes were identified through a genome-wide survey and classified into six groups. Phylogenetic analyses revealed that the TaASR proteins in the same group tightly clustered together, compared with those from other species. Duplication analysis indicated that the TaASR gene family has expanded mainly through tandem and segmental duplication events. Similar gene structures and conserved protein motifs of TaASRs in wheat were identified in the same groups. ASR genes contained various TF binding cites associated with the stress responses in the promoter region. Gene expression was generally associated with the expected group-specific expression pattern in five tissues, including grain, leaf, root, spike and stem, indicating the broad conservation of ASR genes function during wheat evolution. The qRT-PCR analysis revealed that several ASRs were up-regulated in response to NaCl and PEG stress. CONCLUSION: We identified ASR genes in wheat and found that gene duplication events are the main driving force for ASR gene evolution in wheat. The expression of wheat ASR genes was modulated in responses to multiple abiotic stresses, including drought/osmotic and salt stress. The results provided important information for further identifications of the functions of wheat ASR genes and candidate genes for high abiotic stress tolerant wheat breeding.
Descritores: Estresse Fisiológico/genética
Triticum/genética
Ácido Abscísico/análise
Genoma de Planta/genética
Evolução Molecular
Secas
-Filogenia
Fatores de Transcrição/genética
Triticum/classificação
Regulação da Expressão Gênica de Plantas
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


  4 / 35 LILACS  
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Id: lil-692187
Autor: Carrasco, Basilio; Meisel, Lee; Gebauer, Marlene; Garcia-Gonzales, Rolando; Silva, Herman.
Título: Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective
Fonte: Biol. Res;46(3):219-230, 2013. tab.
Idioma: en.
Projeto: CONICYT; . FONDECYT; . CONICYT; . FONDECYT; . CONICYT; . PAI; . Consorcio Tecnológico de la Industria Frutícola S.A.
Resumo: This review is an overview of traditional and modern breeding methodologies being used to develop new Prunus cultivars (stone fruits) with major emphasis on peach, sweet cherry and Japanese plum. To this end, common breeding tools used to produce seedlings, including in vitro culture tools, are discussed. Additionally, the mechanisms of inheritance of many important agronomical traits are described. Recent advances in stone fruit transcriptomics and genomic resources are providing an understanding of the molecular basis of phenotypic variability as well as the identification of allelic variants and molecular markers. These have potential applications for understanding the genetic diversity of the Prunus species, molecular marker-assisted selection and transgenesis. Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNPs) molecular markers are described as useful tools to describe genetic diversity in peach, sweet cherry and Japanese plum. Additionally, the recently sequenced peach genome and the public release of the sweet cherry genome are discussed in terms of their applicability to breeding programs.
Descritores: Variação Genética
Genoma de Planta/genética
Melhoramento Vegetal
Prunus/genética
Transcriptoma/genética
-Alelos
Genótipo
Fenótipo
Prunus/fisiologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: lil-640523
Autor: Shan, Shi-hua; Zhang, Ting-ting; Li, Chun-juan; Yang, Chen; Yan, Cai-xia; Wan, Shu-bo.
Título: Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L. )
Fonte: Electron. j. biotechnol;14(6):6-6, Nov. 2011. ilus, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Modern Agro-industry Technology Research System; . National Key Project of Scientific and Technical Supporting Programs of China.
Resumo: Background: Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66 percent). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties.
Descritores: Arachis/genética
Doenças das Plantas/genética
Genes de Plantas
Imunidade Inata/genética
-DNA Complementar/genética
Clonagem Molecular
Biologia Computacional
Genoma de Planta
Reação em Cadeia da Polimerase/métodos
Responsável: CL1.1 - Biblioteca Central


  6 / 35 LILACS  
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Id: lil-559592
Autor: Feng, Tu; Liu, Shuang; He, Xing-jin.
Título: Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA / Autentificación molecular de la planta medicinal tradicional china Angelica sinensis basada en el espaciador interno transcrito del ADNnr
Fonte: Electron. j. biotechnol;13(1):9-10, Jan. 2010. ilus, tab.
Idioma: en.
Projeto: Nationally Natural Science Foundation of China; . National Infrastructure of Natural Resources for Science and Technology.
Resumo: Traditionally, the authentication of the traditional Chinese medicines (TCM), Angelica sinensis, is based on slightly different morphological characters and complex compounds. Usually, those methods are simultaneously affected by several factors, leading to subtle and ambiguous results. In this study, the internal transcribed spacer (ITS) regions of A. sinensis and seven other Angelica species used as adulterants were sequenced. A pair of specific primers was designed from the polymorphic ITS regions to distinguish A. sinensis from the adulterants and regional substitutes. These ITS-derived primers amplified approximately 520 bp specific fragments from the adulterants, whereas no products was amplified with the DNA of A. sinensis. We tested eight commercially crude materials purchased in the market by using these specific primers. The result showed that there were four samples adulterating A. sinensis with regional substitutes. This indicated that A. sinensis could be accurately distinguished from the adulterants and regional substitutes. Therefore, the method of molecular authentication based on the ITS sequences may be contributed to raw material production and quality control of A. sinensis.
Descritores: DNA Ribossômico/análise
DNA Ribossômico/genética
Angelica sinensis/genética
Angelica sinensis/ultraestrutura
-Análise Citogenética/métodos
Cromossomos de Plantas
Genoma de Planta/genética
Medicina Tradicional Chinesa/métodos
Reação em Cadeia da Polimerase/métodos
Análise de Sequência de DNA
Responsável: CL1.1 - Biblioteca Central


  7 / 35 LILACS  
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Bonetti, Ana Maria
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Id: lil-556812
Autor: Londe, Luciana N; Ueira-Vieira, Carlos; Kerr, Warwick E; Bonetti, Ana Maria.
Título: Characterization of DNA polymorphisms in Caryocar brasiliense (Camb.) in populations with and without thorn at the endocarp by RAPD markers
Fonte: An. acad. bras. ciênc;82(3):779-789, Sept. 2010. ilus, mapas, tab.
Idioma: en.
Resumo: Caryocar brasiliense (pequi), is one of the main species at the biome of the Brazilian savannah due to its use in culinary, popular medicine, industry in general, and iron and steel industry. At São José do Xingu (MT), a tree of C. brasiliense without thorn at the endocarp was found, which enables the improvement of C. brasiliense not only for consumption but also to the high appreciation it already has. To detect the existing differences between the pequi with and without the thorn at the endocarp, RADP markers were used. The generated polymorphisms were cloned and sequenced in order to identify the sequences that are responsible for the fenotypical alteration. It was observed that the pequi without thorn is genetically isolated from the other populations of pequi with thorn at the endocarp, proving that this characteristic is related to the genetic divergence of the species. Analysis in BLASTn evidenced the similarity of the Dof1 genes of Zea mays to its gene of phosphinotricin acetyl transferase. In the analysis of BLASTx, the similarity was verified to the proteins responsible for the deficiency in ferric reductase 4, and catalase.

Pequi, Caryocar brasiliense, é uma das espécies de destaqueno bioma do cerrado brasileiro, devido a sua utilização na medicina, na culinária popular, indústria em geral, e na do ferro e do aço. Na região de São José do Xingu (MT), uma árvore de pequi sem espinho no endocarpo foi encontrado e isso permite melhorar pequi não só para o consumo, aproveitando a alta apreciação que já possui. Para detectar as diferenças existentes entre o genoma de pequi com e sem espinho no endocarpo, marcadores moleculares RAPD foram utilizados. Os polimorfismos gerados foram clonados e sequenciados, a fim de identificar as sequências responsáveis pela alteração fenotípica. Observou-se que o pequi sem espinho é geneticamente isolado de outras populações de pequi com espinho no endocarpo, provando que essa característica está relacionada com a divergência genética da espécie. Análise em Blastn evidenciou a similaridade dos genes Dof1 e com o gene da fosfinotricina-acetiltransferase de Z. mays. Na análise da BLASTx, a similaridade foi verificada com as proteínas responsáveis pela deficiência de ferro 4 redutase e catalase.
Descritores: DNA de Plantas/genética
Genoma de Planta/genética
Polimorfismo Genético/genética
Ericales/genética
-Técnica de Amplificação ao Acaso de DNA Polimórfico
Ericales/anatomia & histologia
Ericales/classificação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  8 / 35 LILACS  
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Id: lil-553785
Autor: Rangel, P. N; Brondani, R. P. V; Rangel, P. H. N; Brondani, C.
Título: Agronomic and molecular characterization of introgression lines from the interspecific cross Oryza sativa (BG90-2) x Oryza glumaepatula (RS-16)
Fonte: Genet. mol. res. (Online);7(1):184-195, Jan. 2008. ilus, tab.
Idioma: en.
Resumo: The reduced genetic variability of modern rice varieties (Oryza sativa) is of concern because it reduces the possibilities of genetic gain in breeding programs. Introgression lines (ILs) containing genomic fragments from wild rice can be used to obtain new improved cultivars. The objective of the present study was to perform the agronomic and molecular characterizations of 35 BC2F8 ILs from the cross O. glumaepatula x O. sativa, aiming to select high-yielding ILs to be used in rice-breeding programs. All 35 ILs were field evaluated in the season 2002/2003 in three locations and the 15 best performing ones were evaluated in the season 2003/2004 in five locations. In 2003/2004, six ILs (CNAi 9934, CNAi 9931, CNAi 9930, CNAi 9935, CNAi 9936, and CNAi 9937) showed the highest yield means and were statistically superior to the controls Metica 1 and IRGA 417. Molecular characterization of the 35 ILs was performed with 92 microsatellite markers distributed on the 12 rice chromosomes and a simple regression Oriza glumaepatula-derived introgression lines quantitative trait locus analysis was performed using the phenotypic data from 2002/2003. The six high-yielding ILs showed a low proportion of wild fragment introgressions. A total of 14 molecular markers were associated with quantitative trait loci in the three locations. The six high-yielding ILs were incorporated in the Embrapa breeding program, and the line CNAi 9930 is recommended for cultivation due to additional advantages of good grain cooking and milling qualities and high yield stability. The O. glumaepatula-derived ILs proved to be a source of new alleles for the development of high-yielding rice cultivars.
Descritores: Agricultura/métodos
Hibridização Genética
Oryza/genética
Locos de Características Quantitativas
-Genes de Plantas
Genoma de Planta
Repetições de Microssatélites
Responsável: BR26.1 - Biblioteca Central


  9 / 35 LILACS  
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Id: lil-551880
Autor: Zhao, Ming; Zhou, Jin yan; Li, Zhi dong; Song, Wei wei; Tan, You jiu; Tan, Hong.
Título: Boty-II, a novel LTR retrotransposon in Botrytis cinerea B05.10 revealed by genomic sequence
Fonte: Electron. j. biotechnol;12(3):2-3, July 2009. ilus, tab.
Idioma: en.
Projeto: Chinese Academy of Sciences; . Hi-Tech Research and Development Program (863) of China.
Resumo: Botrytis cinerea is a necrotrophic pathogen causing pre- and post-harvest diseases in at least 235 plant species. It manifests extraordinary genotype and phenotype variation. One of the causes of this variation is transposable elements. Two transposable elements have been discovered in this fungus, the retrotransposon (Boty), and the transposon (Flipper). In this work, two complete (Boty-II-76 and Boty-II-103) and two partial (Boty-II-95 and Boty-II-141) long terminal repeat (LTR) retrotransposons were identified by an in silico genomic sequence analysis. Boty-II-76 and Boty-II-103 contain 6439 bp nucleotides with a pair of LTRs at both ends, and an internal deduced pol gene encoding a polyprotein with reverse transcriptase and DDE integrase domains. They are flanked by 5 bp direct repeats (ACCAT, CTTTC). In Boty-II-141, two LTRs at both ends, and a partial internal pol gene encoding a protein with a DDE integrase domain were identified. In Boty-II-95, a right LTR and a partial internal pol gene encoding a protein with no conserved domains were identified. Boty-II uses a self-priming mechanism to initiate synthesis of reverse transcripts. The sequence of the presumed primer binding site for first-strand reverse transcription is 5'-TTGTACCAT-3'. The polypurine-rich sequence for plus-strand DNA synthesis is 5'-GCCTTGAGCGGGGGGTAC-3'. Fourteen Boty-II LTRs that contain 125-158 bp nucleotides and share 69.1 ~ 100 percent identities with the short inverted terminal repeats of 5 bp (TGTCA…TGACA) were discovered. Analysis of structural features and phylogeny revealed that Boty-II is a novel LTR retrotransposon. It could potentially be used as a novel molecular marker for the investigation of genetic variation in B. cinerea.
Descritores: Botrytis/isolamento & purificação
Botrytis/genética
Botrytis/química
Retroelementos/genética
-Variação Genética
Genoma de Planta/genética
Saccharomyces cerevisiae/enzimologia
Saccharomyces cerevisiae/química
Responsável: CL1.1 - Biblioteca Central


  10 / 35 LILACS  
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Id: lil-538012
Autor: Rojas, Gabriela; Méndez, Marco A; Muñoz, Carlos; Lemus, Gamalier; Hinrichsen, Patricio.
Título: Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
Fonte: Electron. j. biotechnol;11(5):4-5, Dec. 2008. ilus, tab.
Idioma: en.
Projeto: FONDEF.
Resumo: The genetic characterization of 117 peach and nectarine cultivars (Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. Analyzed genotypes include the complete list of cultivars under intellectual property (IP) protection in Chile. One hundred and two out of the 117 cultivars under study could be identified using only 7 SSRs. Other 5 cultivars were differentiated using 3 additional markers, but 5 pairs of genotypes were not differentiable. The average expected heterozygosity for the set of markers was 0.55, ranging from 0.28 in BPPCT-008 to 0.81 in CPPCT-022, with an F value of 0.37. A Neighbor-Joining dendrogram showed that, with few exceptions, peaches and nectarines clustered separately. These results are the basis for the development of a fingerprinting protocol for the unequivocal identification of most of the peach and nectarine cultivars officially registered in Chile.
Descritores: Cultivos Agrícolas
Amygdalus persica/isolamento & purificação
Genoma de Planta
Pyrus/genética
-Impressões Digitais de DNA
Repetições de Microssatélites/genética
Clima Temperado
Responsável: CL1.1 - Biblioteca Central



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