Base de dados : LILACS
Pesquisa : G05.360.340.550 [Categoria DeCS]
Referências encontradas : 13 [refinar]
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Id: biblio-974310
Autor: Souza, Renata Carolini; Cantão, Maurício Egídio; Nogueira, Marco Antonio; Vasconcelos, Ana Tereza Ribeiro; Hungria, Mariangela.
Título: Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach
Fonte: Braz. j. microbiol;49(4):723-730, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: CNPq-Universal; . CNPq; . Embrapa.
Resumo: ABSTRACT The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspot for bioprospection of hydrolases.
Descritores: Solo/química
Proteínas Fúngicas/genética
Archaea/enzimologia
Proteínas Arqueais/genética
Fungos/enzimologia
Hidrolases/genética
-Microbiologia do Solo
Soja/crescimento & desenvolvimento
Triticum/crescimento & desenvolvimento
Brasil
Archaea/isolamento & purificação
Archaea/classificação
Archaea/genética
Zea mays/crescimento & desenvolvimento
Agricultura
Metagenoma
Metagenômica
Fungos/isolamento & purificação
Fungos/classificação
Fungos/genética
Responsável: BR1.1 - BIREME


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Id: biblio-828196
Autor: Medeiros, Julliane Dutra; Cantão, Maurício Egídio; Cesar, Dionéia Evangelista; Nicolás, Marisa Fabiana; Diniz, Cláudio Galuppo; Silva, Vânia Lúcia; Vasconcelos, Ana Tereza Ribeiro de; Coelho, Cíntia Marques.
Título: Comparative metagenome of a stream impacted by the urbanization phenomenon
Fonte: Braz. j. microbiol;47(4):835-845, Oct.-Dec. 2016. graf.
Idioma: en.
Resumo: Abstract Rivers and streams are important reservoirs of freshwater for human consumption. These ecosystems are threatened by increasing urbanization, because raw sewage discharged into them alters their nutrient content and may affect the composition of their microbial community. In the present study, we investigate the taxonomic and functional profile of the microbial community in an urban lotic environment. Samples of running water were collected at two points in the São Pedro stream: an upstream preserved and non-urbanized area, and a polluted urbanized area with discharged sewage. The metagenomic DNA was sequenced by pyrosequencing. Differences were observed in the community composition at the two sites. The non-urbanized area was overrepresented by genera of ubiquitous microbes that act in the maintenance of environments. In contrast, the urbanized metagenome was rich in genera pathogenic to humans. The functional profile indicated that the microbes act on the metabolism of methane, nitrogen and sulfur, especially in the urbanized area. It was also found that virulence/defense (antibiotic resistance and metal resistance) and stress response-related genes were disseminated in the urbanized environment. The structure of the microbial community was altered by uncontrolled anthropic interference, highlighting the selective pressure imposed by high loads of urban sewage discharged into freshwater environments.
Descritores: Urbanização
Microbiologia da Água
Rios/microbiologia
Metagenoma
Microbiota
-Filogenia
RNA Ribossômico 16S/genética
Ecossistema
Metabolismo Energético
Redes e Vias Metabólicas
Metagenômica
Código de Barras de DNA Taxonômico
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-889172
Autor: Song, Yun-Hee; Lee, Kyung-Tai; Baek, Jin-Young; Kim, Min-Ju; Kwon, Mi-Ra; Kim, Young-Joo; Park, Mi-Rim; Ko, Haesu; Lee, Jin-Sung; Kim, Keun-Sung.
Título: Isolation and characterization of a novel endo-beta-1, 4-glucanase from a metagenomic library of the black-goat rumen
Fonte: Braz. j. microbiol;48(4):801-808, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized. The novel glycosyl hydrolase family 5 cellulase gene is composed of a 963-bp open reading frame encoding a protein of 320 amino acid residues (35.1 kDa). The deduced amino acid sequence showed the highest sequence identity (58%) for sequences from the glycosyl hydrolase family 5 cellulases. The novel glycosyl hydrolase family 5 cellulase gene was overexpressed in Escherichia coli. Substrate specificity analysis revealed that this recombinant glycosyl hydrolase family 5 cellulase functions as an endo-β-1,4-glucanase. The recombinant KG35 endo-β-1,4-glucanase showed optimal activity within the range of 30-50 °C at a pH of 6-7. The thermostability was retained and the pH was stable in the range of 30-50 °C at a pH of 5-7.
Descritores: Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Bactérias/enzimologia
Celulase/química
Celulase/genética
Rúmen/microbiologia
-Proteínas de Bactérias/metabolismo
Bactérias/classificação
Bactérias/genética
Bactérias/isolamento & purificação
Celulase/metabolismo
Clonagem Molecular
Estabilidade Enzimática
Microbioma Gastrointestinal
Cabras
Concentração de Íons de Hidrogênio
Metagenoma
Metagenômica
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-839234
Autor: Thimoteo, SS; Glogauer, A; Faoro, H; de Souza, EM; Huergo, LF; Moerschbacher, BM; Pedrosa, FO.
Título: A broad pH range and processive chitinase from a metagenome library
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(1):e5658, 2017. tab, graf.
Idioma: en.
Projeto: INCT-FBN/CNPq/MCT.
Resumo: Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme retained at least 80% of its activity in the pH range from 4 to 9 and 98% at 600 mM NaCl. Thin layer chromatography analyses identified chitobiose as the main product of MetaChi18A on chitin polymers as substrate. Kinetic analysis showed inhibition of MetaChi18A activity at high concentrations of colloidal chitin and 4-methylumbelliferyl N,N′-diacetylchitobiose and sigmoid kinetics at low concentrations of colloidal chitin, indicating a possible conformational change to lead the chitin chain from the chitin-binding to the catalytic domain. The observed stability and activity of MetaChi18A over a wide range of conditions suggest that this chitinase, now characterized, may be suitable for application in the industrial processing of chitin.
Descritores: Quitinases/genética
Quitina/genética
Metagenoma/genética
-Quitinases/química
Quitina/química
Cromatografia Líquida de Alta Pressão
Escherichia coli
Expressão Gênica/genética
Biblioteca Gênica
Vetores Genéticos
Concentração de Íons de Hidrogênio
Especificidade por Substrato
Responsável: BR1.1 - BIREME


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Id: lil-748271
Autor: Martinez, Bruno Prata; Gomes, Isabela Barboza; Oliveira, Carolina Santana de; Ramos, Isis Resende; Rocha, Mônica Diniz Marques; Forgiarini Júnior, Luiz Alberto; Camelier, Fernanda Warken Rosa; Camelier, Aquiles Assunção.
Título: Accuracy of the Timed Up and Go test for predicting sarcopenia in elderly hospitalized patients
Fonte: Clinics;70(5):369-372, 05/2015. tab, graf.
Idioma: en.
Resumo: OBJECTIVES: The ability of the Timed Up and Go test to predict sarcopenia has not been evaluated previously. The objective of this study was to evaluate the accuracy of the Timed Up and Go test for predicting sarcopenia in elderly hospitalized patients. METHODS: This cross-sectional study analyzed 68 elderly patients (≥60 years of age) in a private hospital in the city of Salvador-BA, Brazil, between the 1st and 5th day of hospitalization. The predictive variable was the Timed Up and Go test score, and the outcome of interest was the presence of sarcopenia (reduced muscle mass associated with a reduction in handgrip strength and/or weak physical performance in a 6-m gait-speed test). After the descriptive data analyses, the sensitivity, specificity and accuracy of a test using the predictive variable to predict the presence of sarcopenia were calculated. RESULTS: In total, 68 elderly individuals, with a mean age 70.4±7.7 years, were evaluated. The subjects had a Charlson Comorbidity Index score of 5.35±1.97. Most (64.7%) of the subjects had a clinical admission profile; the main reasons for hospitalization were cardiovascular disorders (22.1%), pneumonia (19.1%) and abdominal disorders (10.2%). The frequency of sarcopenia in the sample was 22.1%, and the mean length of time spent performing the Timed Up and Go test was 10.02±5.38 s. A time longer than or equal to a cutoff of 10.85 s on the Timed Up and Go test predicted sarcopenia with a sensitivity of 67% and a specificity of 88.7%. The accuracy of this cutoff for the Timed Up and Go test was good (0.80; IC=0.66-0.94; p=0.002). CONCLUSION: The Timed Up and Go test was shown to be a predictor of sarcopenia in elderly hospitalized patients. .
Descritores: Rejeição de Enxerto/etiologia
Rejeição de Enxerto/mortalidade
Íleo/microbiologia
Intestino Delgado/transplante
Intestinos/microbiologia
Complicações Pós-Operatórias
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
-Seguimentos
Rejeição de Enxerto/diagnóstico
Intestino Delgado/patologia
Intestino Delgado/cirurgia
Metagenoma/genética
Prognóstico
Estudos Prospectivos
Fatores de Risco
Taxa de Sobrevida
Limites: Adolescente
Adulto
Criança
Pré-Escolar
Feminino
Humanos
Lactente
Masculino
Pessoa de Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-708089
Autor: Llanos Villarreal, Jenny.
Título: El estudio de nuestro microcosmos: las comunidades microbianas asociadas al cuerpo humano / The study of our microcosm: the microbial community related to the human body
Fonte: Bol. micol. (Valparaiso En linea);28(2):71-77, dic. 2013. tab.
Idioma: es.
Resumo: El cuerpo humano presenta una gran variedad de micro nichos que están colonizados por diversos grupos de microorganismos, principalmente bacterias, que establecen con nuestro cuerpo una relación simbionte y colaboran de manera positiva a nuestrabiología. En los últimos años, diversos grupos multinacionales han emprendido la tarea de estudiar, mediante avanzados procedimientos de análisis molecular y bioinformáticos, las comunidades microbianas que habitan nuestro cuerpo. Este artículo, pretende entregar una visión global de cómo, en la actualidad, se está abordando el estudio de nuestromicrocosmos y cuáles son los aspectos más sobresalientes derivados de los mismos.

The human body has great variety of micro niches that are colonized by various groups of microorganisms, mainly bacteria, which provide ourbody with a symbiotic relationship and collaborate in a positive way to our biology. In recent years, variousmultinational groups have undertaken the task of studying, using advanced methods of molecular and bioinformatic analysis, microbial communitiesinhabiting our bodies. This article aims to give an overview of how, at present, is being addressed the study of our microcosm and what aspects are mostover projections derived from them.
Descritores: Corpo Humano
Intestinos/microbiologia
Metagenoma
Interações Microbianas
Pele/microbiologia
Vagina/microbiologia
Limites: Humanos
Responsável: CL2.1 - Biblioteca de Medicina


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Id: lil-656832
Autor: Jaramillo, Adriana; Arce, Roger; Contreras, Adolfo; Herrera, Julián A.
Título: Efecto del tratamiento periodontal sobre la microbiota subgingival en pacientes con preeclampsia / Effect of periodontal therapy on the subgingival microbiota in preeclamptic patients
Fonte: Biomédica (Bogotá);32(2):233-238, abr.-jun. 2012. ilus, tab.
Idioma: es.
Resumo: Introducción. Pocos estudios han descrito la microbiota subgingival en mujeres embarazadas con preeclampsia leve. Objetivo. Identificar cambios periodontales y de la microbiota subgingival en mujeres embarazadas con preeclampsia, después del tratamiento periodontal. Materiales y métodos. En un análisis secundario de un ensayo clínico de asignación aleatoria, se estudiaron 57 pacientes con preeclampsia en el Hospital Universitario del Valle de Cali. Se asignaron al azar 31 al grupo de intervención periodontal (detartraje y alisado subgingival ultrasónico y manual) durante su embarazo y otras 26 al grupo control (profilaxis supragingival). Se determinaron los parámetros clínicos periodontales y la microbiota subgingival a la inclusión al estudio y en el posparto. Se evaluaron 8 bacterias periodontopáticas y 2 virus herpes por reacción en cadena de la polimerasa. Se usaron las pruebas de ji al cuadrado, test de McNemar o t de Student, con un nivel de significancia de p≤ Resultados. Los grupos fueron comparables en las variables clínicas y microbiológicas al inicio del estudio. El tratamiento periodontal redujo el promedio de la profundidad de bolsa en el grupo de intervención de 2,44±0,31 a 2,31±0,24 mm (p=0,000) y en el grupo control de 2,58±0,37 a 2,44±0,39 mm (p=0,000),y el índice de sangrado, de 16,4±1,5 a 7,9±0,7 % en el primero (p=0,000), y de 17,1±1,8 a 10±0,9 %, en el segundo (p=0,002). La frecuencia de detección de microorganismos no varió de manera significativa entre los grupos. Conclusión. El raspaje y alisado radicular, así como la profilaxis supragingival, redujeron de manera significativa la profundidad a la sonda y el índice de sangrado gingival. El tratamiento periodontal no fue más efectivo que la profilaxis para reducir los organismos periodontopáticos o los virus herpes.

Introduction. Few studies have described subgingival microbiota in pregnant women with mild preeclampsia. Objective. Clinical periodontal and subgingival microbiota changes were identified in pregnant women with mild preeclampsia after periodontal treatment. Materials and methods. In a secondary analysis of a randomized clinical trial, 57 preeclamptic women were studied at Hospital Universitario del Valle in Cali, Colombia. Thirty one women were randomized to the periodontal intervention group (subgingival scaling and planing ultrasonic and manual) during pregnancy and 26 to the control group (supragingival prophylaxis). Periodontal clinical parameters and subgingival microbiota were characterized at the time of acceptance into the study and again at postpartum. Eight periodontopathic bacteria and 2 herpesviruses were assessed by polymerase chain reaction. Chi-square, McNemar or Student´s t tests were used, with a significance level of p≤0.05. Results. Both groups were comparable in the clinical and microbiological variables at baseline. Periodontal treatment reduced the average pocket depth in the intervention group from 2.4±0.3 to 2.3±0.2 mm (p<0.001) and in control group 2.6±0.4 to 2.44±0.4 mm, (p<0.001) and bleeding index 16.4±1.5% to 7.9±0.7% in the intervention group(p<0.001) and 17.1±1.8% to 10±0.9% in the control group (p=0.002). The frequency of detection of microorganisms did not differ significantly between groups. Conclusion. Scaling/root planning and supragingival prophylaxis significantly reduced the probing depth and gingival bleeding index. Periodontal treatment was not more effective than prophylaxis in reducing periodontopathic organisms or herpesvirus.
Descritores: Raspagem Dentária
Metagenoma
Pré-Eclâmpsia/microbiologia
Aplainamento Radicular
-Bactérias/isolamento & purificação
Polimento Dentário
Raspagem Dentária/métodos
Hemorragia Gengival/etiologia
Gengivite/complicações
Gengivite/microbiologia
Gengivite/prevenção & controle
Gengivite/terapia
Gengivite/virologia
/isolamento & purificação
HERPESVIRUS ABBREVIATIONS AS TOPIC, HUMAN/isolamento & purificação
Higiene Bucal
Educação de Pacientes como Assunto
Bolsa Periodontal/microbiologia
Bolsa Periodontal/prevenção & controle
Bolsa Periodontal/virologia
Periodontite/complicações
Periodontite/microbiologia
Periodontite/prevenção & controle
Periodontite/terapia
Periodontite/virologia
Complicações Infecciosas na Gravidez/microbiologia
Complicações Infecciosas na Gravidez/prevenção & controle
Complicações Infecciosas na Gravidez/terapia
Complicações Infecciosas na Gravidez/virologia
Transtornos Puerperais/microbiologia
Transtornos Puerperais/virologia
Aplainamento Radicular/métodos
Índice de Gravidade de Doença
Simplexvirus/isolamento & purificação
Limites: Adulto
Feminino
Humanos
Gravidez
Tipo de Publ: Estudo Comparativo
Ensaio Clínico Controlado Aleatório
Research Support, Non-U.S. Gov't
Responsável: CO332 - Facultad de Medicina


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Id: lil-640713
Autor: Artese, Hilana Paula Carillo; Sousa, Celso Oliveira de; Torres, Maria Cynésia Medeiros de Barros; Silva-Boghossian, Carina Maciel; Colombo, Ana Paula Vieira.
Título: Effect of non-surgical periodontal treatment on the subgingival microbiota of patients with chronic kidney disease
Fonte: Braz. oral res;26(4):366-372, July-Aug. 2012. graf, tab.
Idioma: en.
Resumo: This study investigated the effect of non-surgical periodontal therapy on the composition of subgingival microbiota of patients with chronic kidney disease (CKD). Sixteen CKD pre-dialysis individuals (CKD) and 14 individuals without clinical evidence of kidney disease (C) presenting chronic periodontitis were treated by scaling and root planing. Subgingival samples were collected from each patient and analyzed for their composition by checkerboard at baseline and 3 months post-therapy. Significant differences between groups at baseline were sought by the Mann-Whitney and χ² tests. Changes over time were examined by the Wilcoxon test. At baseline, the CKD group had significantly lower counts of E. faecalis compared to the C group (p < 0.05). After treatment, the levels of a greater number of species were reduced in the C group. Higher levels of A. israelii, C. rectus, F. periodonticum, P. micra, P. nigrescens, T. forsythia, N. mucosa, and S. anginosus (p < 0.05) were found in the CKD group compared to the C group. Also, non-responsive sites in CKD individuals harbored significantly higher levels of pathogenic species (T. forsythia, P. gingivalis, T. denticola, Fusobacterium spp., D. pneumosintes, E. faecalis and S. aureus; p < 0.05) than sites that responded to therapy, as well as non-responsive sites in the C group. The periodontitis-associated subgingival microbiota of CKD and systemically healthy individuals was similar in composition. However, high levels of pathogenic species persisted in the subgingival microbiota of patients with CKD after treatment.
Descritores: Gengiva/microbiologia
Periodontite/terapia
Insuficiência Renal Crônica/microbiologia
-Carga Bacteriana
Doença Crônica
Raspagem Dentária
Sondas de DNA
Metagenoma
Periodontite/imunologia
Insuficiência Renal Crônica/imunologia
Estatísticas não Paramétricas
Fatores de Tempo
Resultado do Tratamento
Limites: Idoso
Feminino
Humanos
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-634699
Autor: Bologno, Romina; Díaz, Yanina M; Giraudo, María C; Fernández, Rosa; Menéndez, Viviana; Brizuela, Juan C; Gallardo, Adriana A; Álvarez, Laura A; Estevao Belchior, Silvia G.
Título: Importancia del estudio del balance del contenido vaginal (BACOVA) en el control preventivo de las trabajadoras sexuales / Importance of studying the balance of vaginal content (BAVACO) in the preventive control of sex workers
Fonte: Rev. argent. microbiol;43(4):246-250, dic. 2011. tab.
Idioma: es.
Resumo: El objetivo de este trabajo fue estudiar a un grupo de 229 trabajadoras sexuales de Comodoro Rivadavia (Chubut), atendidas en centros públicos de salud de dicha ciudad, mediante la aplicación del método conocido como balance del contenido vaginal (BACOVA). Este método comprende el estudio morfológico de la microbiota vaginal, como así también de la reacción infamatoria. Incluye el análisis del contenido vaginal en fresco y por tinciones de Gram y de Giemsa, de modo de integrar la exploración de todo el panorama biológico. El 35,37 % de estas mujeres presentó microbiota normal (MN); el 15,72 %, microbiota intermedia (MI); el 23,14 %, vaginosis bacteriana (VB) y el 10,48 %, vaginitis microbiana inespecífca (VMI). Los casos de vaginitis por levaduras y por Trichomonas vaginalis comprendieron el 8,30 % y 6,99 % de las mujeres, respectivamente. Se observó el desplazamiento de la MN hacia una MI, que se correspondió con el predominio de bacterias corineformes. Por otra parte, no se reconoció un marcado desequilibrio del contenido vaginal ante la colonización e infección por levaduras o por T. vaginalis: el 48 % de los casos de estas vaginitis convencionales no presentaron reacción infamatoria vaginal (RIV). El 24,89 % de los casos de MN presentaron una signifcativa RIV, y en más del 50 % de las mujeres se diagnosticaron disfunciones vaginales en ausencia de sintomatología. Estos resultados se podrían asociar a un incremento del riesgo gineco-obstétrico, lo que afecta la salud sexual y reproductiva de la población estudiada.

The aim of this work was to study the vaginal microenvironment in sex workers from Comodoro Rivadavia, Chubut. For that purpose, BAVACO procedures were applied. A total of 229 female sex workers attended public health centers. Vaginal secretions were analyzed by Gram and Giemsa stains. The following results were obtained: normal microbiota 35.37 %, intermediate microbiota 15.72 %, bacterial vaginosis 23.14 %, microbial nonspecifc vaginitis, Donders'"aerobic vaginitis" 10.48 %, yeast vulvovaginitis 8.30 %, and trichomoniasis 6.99 %. The intermediate microbiota was characterized by a decrease in the number of lactobacilli and the presence of diphtheroid bacilli cell types. The population studied shared increased values of vaginal dysfunctions. These results are considered risk factors for obstetric and gynecologic diseases.
Descritores: Candidíase Vulvovaginal/prevenção & controle
Metagenoma
Doenças Profissionais/prevenção & controle
Profissionais do Sexo
Doenças Bacterianas Sexualmente Transmissíveis/prevenção & controle
Vagina/microbiologia
-Distribuição por Idade
Argentina
Líquidos Corporais/microbiologia
Líquidos Corporais/parasitologia
Estudos Transversais
Candidíase Vulvovaginal/epidemiologia
Candidíase Vulvovaginal/microbiologia
Doenças Profissionais/epidemiologia
Doenças Profissionais/microbiologia
Profissionais do Sexo/estatística & dados numéricos
Doenças Bacterianas Sexualmente Transmissíveis/epidemiologia
Doenças Bacterianas Sexualmente Transmissíveis/microbiologia
Vaginite por Trichomonas/epidemiologia
Vaginite por Trichomonas/parasitologia
Vaginite por Trichomonas/prevenção & controle
Vagina/parasitologia
Vaginose Bacteriana/epidemiologia
Vaginose Bacteriana/microbiologia
Vaginose Bacteriana/prevenção & controle
Limites: Adulto
Idoso
Feminino
Humanos
Pessoa de Meia-Idade
Adulto Jovem
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-634678
Autor: Berlemont, Renaud; Pipers, Delphine; Delsaute, Maud; Angiono, Federico; Feller, Georges; Galleni, Moreno; Power, Pablo.
Título: Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements / Explorando el metagenoma del suelo antártico como fuente de nuevas enzimas adaptadas al frío y elementos génicos móviles
Fonte: Rev. argent. microbiol;43(2):94-103, jun. 2011. ilus, graf, tab.
Idioma: en.
Projeto: FNRS; . CONICET. PIP 11220090100533.
Resumo: Metagenomic library PP1 was obtained from Antarctic soil samples. Both functional and genotypic metagenomic screening were used for the isolation of novel cold-adapted enzymes with potential applications, and for the detection of genetic elements associated with gene mobilization, respectively. Fourteen lipase/esterase-, 14 amylase-, 3 protease-, and 11 cellulase-producing clones were detected by activity-driven screening, with apparent maximum activities around 35 °C for both amylolytic and lipolytic enzymes, and 35-55 °C for cellulases, as observed for other cold-adapted enzymes. However, the behavior of at least one of the studied cellulases is more compatible to that observed for mesophilic enzymes. These enzymes are usually still active at temperatures above 60 °C, probably resulting in a psychrotolerant behavior in Antarctic soils. Metagenomics allows to access novel genes encoding for enzymatic and biophysic properties from almost every environment with potential benefits for biotechnological and industrial applications. Only intI- and tnp-like genes were detected by PCR, encoding for proteins with 58-86 %, and 58-73 % amino acid identity with known entries, respectively. Two clones, BAC 27A-9 and BAC 14A-5, seem to present unique syntenic organizations, suggesting the occurrence of gene rearrangements that were probably due to evolutionary divergences within the genus or facilitated by the association with transposable elements. The evidence for genetic elements related to recruitment and mobilization of genes (transposons/integrons) in an extreme environment like Antarctica reinforces the hypothesis of the origin of some of the genes disseminated by mobile elements among "human-associated" microorganisms.

A partir de muestras de suelo antártico se obtuvo la metagenoteca PP1. Esta fue sometida a análisis funcionales y genotípicos para el aislamiento de nuevas enzimas adaptadas al frío con potenciales aplicaciones, y para la detección de elementos génicos asociados a la movilización de genes, respectivamente. Por tamizaje fenotípico se detectaron 14, 14, 3 y 11 clones productores de lipasas/esterasas, proteasas, amilasas y celulasas, respectivamente, con actividades máximas aparentes de 35 °C para las amilasas y lipasas, y de 35-55 °C para las celulasas, tal como se observó para otras enzimas adaptadas al frío. Sin embargo, una celulasa parece ser compatible con enzimas mesófilas, las que usualmente se mantienen activas hasta por sobre 60 °C. Este hecho probablemente esté asociado a un comportamiento psicrotolerante en los suelos antárticos. La metagenómica permite acceder a una nueva miríada de productos metabólicos con potenciales beneficios para aplicaciones biotecnológicas e industriales. Se detectaron los genes tipo intI y tnp por PCR, y sus productos génicos deducidos tuvieron identidades del 58 al 86 % y del 58 al 73 % con secuencias conocidas, respectivamente. Dos clones, BAC 27A-9 y BAC 14A-5, parecen presentar organizaciones sintéticas únicas, lo cual sugiere la existencia de rearreglos génicos probablemente debidos a divergencias evolutivas dentro del género o facilitados por la asociación de elementos de transposición. La evidencia de elementos génicos relacionados con el reclutamiento y la movilización de genes en ambientes extremos como la Antártida refuerza la hipótesis sobre el origen de algunos genes diseminados por elementos móviles entre los microorganismos asociados al ser humano.
Descritores: Clima Frio
Enzimas/genética
Sequências Repetitivas Dispersas/genética
Metagenoma
Microbiologia do Solo
-Adaptação Fisiológica
Sequência de Aminoácidos
Regiões Antárticas
Clonagem Molecular
Cromossomos Artificiais Bacterianos/genética
Enzimas/isolamento & purificação
Fertilizantes
Gasolina
Biblioteca Gênica
Dados de Sequência Molecular
Petróleo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Poluentes do Solo
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas



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