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Pesquisa : G05.360.600 [Categoria DeCS]
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Id: biblio-951794
Autor: Hernandez-Flores, Jose Luis; Pérez, Juan Caballero; Gutiérrez, Carlos Saldaña; Hernández, Andrés Cruz; Alonso, Gerardo Soto; Hernández, Sergio Pacheco; Gómez, Sergio Romero; Fernández, Francisco; Loske, Achim M; Guillén, Juan Campos.
Título: pMEX01, a 70kb Plasmid Isolated From Escherichia Coli That Confers Resistance to Multiple ß-Lactam Antibiotics
Fonte: Braz. j. microbiol;49(3):569-574, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The strain CM6 is of high epidemiological concern because it not only displays resistance to multiple β-lactam antibiotics but also to other kinds of antibiotics.
Descritores: Plasmídeos/genética
Doenças dos Bovinos/microbiologia
Farmacorresistência Bacteriana
beta-Lactamas/farmacologia
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Infecções por Escherichia coli/veterinária
Antibacterianos/farmacologia
-Plasmídeos/metabolismo
beta-Lactamases/genética
beta-Lactamases/metabolismo
Testes de Sensibilidade Microbiana
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
Escherichia coli/metabolismo
Infecções por Escherichia coli/microbiologia
México
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


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Id: biblio-974322
Autor: Pontes, Patricia Silveira de; Coutinho, Selene Dall' Acqua; Iovine, Renata de Oliveira; Cunha, Marcos Paulo Vieira; Knöbl, Terezinha; Carvalho, Vania Maria de.
Título: Survey on pathogenic Escherichia coli and Salmonella spp. in captive cockatiels (Nymphicus hollandicus)
Fonte: Braz. j. microbiol;49(supl.1):76-82, 2018. tab, graf.
Idioma: en.
Resumo: Abstract We surveyed healthy captive cockatiels (Nymphicus hollandicus) for Escherichia coli and Salmonella spp. Cloacal swabs were collected from 94 cockatiels kept in commercial breeders, private residencies and pet shops in the cities of São Paulo/SP and Niterói/RJ (Brazil). Three strains of E. coli from each individual were tested for the presence of ExPEC-, APEC- and DEC-related genes. We evaluated the blaTEM, blaSHV, blaOXA, blaCMY, blaCTX-M, tetA, tetB, aadA, aphA, strAB, sul1, sul2, sul3, qnrA, qnrD, qnrB, qnrS, oqxAB, aac (6)′-Ib-cr, qepA resistance genes and markers for plasmid incompatibility groups. Salmonella spp. was not detected. E. coli was isolated in 10% of the animals (9/94). Four APEC genes (ironN, ompT, iss and hlyF) were detected in two strains (2/27-7%), and iss (1/27-4%) in one isolate. The highest resistance rates were observed with amoxicillin (22/27-82%), ampicillin (21/27-79%), streptomycin (18/27-67%), tetracycline (11/27-41%). Multiresistance was verified in 59% (16/27) of the isolates. We detected strAB, bla TEM, tetA, tetB, aadA, aphaA, sul1, sul2, sul3 resistance genes and plasmid Inc groups in 20 (74%) of the strains. E. coli isolated from these cockatiels are of epidemiological importance, since these pets could transmit pathogenic and multiresistant microorganisms to humans and other animals.
Descritores: Salmonella/isolamento & purificação
Salmonelose Animal/microbiologia
Doenças das Aves/microbiologia
Cacatuas/microbiologia
Escherichia coli/isolamento & purificação
Infecções por Escherichia coli/veterinária
-Plasmídeos/genética
Plasmídeos/metabolismo
Salmonella/classificação
Salmonella/fisiologia
Salmonella/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Brasil
Farmacorresistência Bacteriana Múltipla
Escherichia coli/classificação
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Infecções por Escherichia coli/microbiologia
Antibacterianos/farmacologia
Limites: Animais
Responsável: BR1.1 - BIREME


  3 / 284 LILACS  
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Id: biblio-974306
Autor: Dealtry, Simone; Ghizelini, Angela Michelato; Mendonça-Hagler, Leda C. S; Chaloub, Ricardo Moreira; Reinert, Fernanda; Campos, Tácio M. P. de; Gomes, Newton C. M; Smalla, Kornelia.
Título: Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana
Fonte: Braz. j. microbiol;49(4):757-769, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Deutsche Forschungsgemeinschaft; . FAPERJ-Brazil.
Resumo: ABSTRACT Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while the bacterial community was rather stable, pronounced shifts were observed in the abundance of bacteria carrying plasmids. A PCR-Southern blot hybridization analysis indicated an increase in the abundance of IncP-9 catabolic plasmids. Denaturing gradient gel electrophoresis of naphthalene dioxygenase (ndo) genes amplified from cDNA (RNA) indicated the dominance of a specific ndo gene in the inoculated petroleum amendment treatment. The petroleum hydrocarbon degrading consortium characterization indicated the prevalence of bacteria assigned to Pseudomonas spp., Comamonas spp. and Ochrobactrum spp. IncP-9 plasmids were detected for the first time in Comamonas sp. and Ochrobactrum spp., which is a novelty of this study.
Descritores: Bactérias/isolamento & purificação
Bactérias/metabolismo
Avicennia/microbiologia
Hidrocarbonetos/metabolismo
-Plasmídeos/genética
Plasmídeos/metabolismo
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
Bactérias/classificação
Bactérias/genética
Biodegradação Ambiental
DNA Bacteriano/genética
Petróleo/análise
RNA Ribossômico 16S/genética
Poluição por Petróleo/análise
Avicennia/metabolismo
Rizosfera
Responsável: BR1.1 - BIREME


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Id: biblio-839327
Autor: Sampaio, Jorge Luiz Mello; Gales, Ana Cristina.
Título: Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on ß-lactams and polymyxins
Fonte: Braz. j. microbiol;47(supl.1):31-37, Oct.-Dec. 2016. tab.
Idioma: en.
Resumo: ABSTRACT During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96.2%) in carbapenem resistant K. pneumoniae. To date KPC-2 is the only variant reported in Brazil. Polymyxin B resistance in KPC-2-producing K. pneumoniae has come to an alarming rate of 27.1% in 2015 in São Paulo, the largest city in Brazil. New Delhi metallo-β-lactamase was detected in Brazil in 2013, has been reported in different Brazilian states but are not widely disseminated. Antimicrobial resistance in Enterobacteriaceae in Brazil is a very serious problem that needs urgent actions which includes both more strict adherence to infection control measures and more judicious use of antimicrobials.
Descritores: Farmacorresistência Bacteriana
Enterobacteriaceae/efeitos dos fármacos
Infecções por Enterobacteriaceae/microbiologia
Infecções por Enterobacteriaceae/epidemiologia
Anti-Infecciosos/farmacologia
-Plasmídeos/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
beta-Lactamases/genética
beta-Lactamases/metabolismo
Brasil/epidemiologia
Polimixinas/uso terapêutico
Polimixinas/farmacologia
beta-Lactamas/uso terapêutico
beta-Lactamas/farmacologia
Enterobacteriaceae/enzimologia
Enterobacteriaceae/genética
Anti-Infecciosos/uso terapêutico
Antibacterianos/uso terapêutico
Antibacterianos/farmacologia
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-839342
Autor: Cortesia, Claudia; Bello, Teresita; Lopez, Gustavo; Franzblau, Scott; Waard, Jacobus de; Takiff, Howard.
Título: Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics
Fonte: Braz. j. microbiol;48(1):151-158, Jan.-Mar. 2017. graf.
Idioma: en.
Projeto: FONACIT.
Resumo: Abstract Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recently been many non-tuberculous mycobacteria soft tissue infections after minor surgical procedures, some apparently related to the use of a commercial disinfectant based on a Quaternary Ammonium Compound. We studied the activity of this and other quaternary ammonium compounds on different non-tuberculous mycobacteria by transforming the mycobacteria with a dnaA-gfp fusion and then monitoring fluorescence to gauge the capacity of different quaternary ammonium compounds to inhibit bacterial growth. The minimum inhibitory concentration varied for the different quaternary ammonium compounds, but M. chelonae and M. abscessus were consistently more resistant than M. smegmatis, and M. terrae more resistant than M. bovis BCG.
Descritores: Expressão Gênica
Proteínas de Fluorescência Verde
Desinfetantes/farmacologia
Compostos de Amônio Quaternário/farmacologia
Antibacterianos/farmacologia
Micobactérias não Tuberculosas/efeitos dos fármacos
-Plasmídeos/genética
Proteínas Recombinantes de Fusão/genética
Testes de Sensibilidade Microbiana
Proteínas de Fluorescência Verde/genética
Relação Dose-Resposta a Droga
Micobactérias não Tuberculosas/classificação
Micobactérias não Tuberculosas/genética
Responsável: BR1.1 - BIREME


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Id: biblio-1046020
Autor: Pinto, Ciro Pedro Guidotti; Rickes, Letícia Neutzling; Zotti, Moisés João; Grutzmacher, Anderson Dionei.
Título: Compared activity of agonist molecules towards ecdysone receptor in insect cell-based screening system / Comparação da atividade de moléculas agonistas em relação ao receptor de ecdisona em um sistema de triagem baseado em linhagens celulares de insetos
Fonte: Arq. Inst. Biol;86:e0312019, 2019. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: The ecdysone receptor, naturally activated by steroidal hormones, is a key protein for molting and reproduction processes of insects. Artificial activation of such receptor by specific pesticides induces an anomalous process of ecdysis, causing death of insects by desiccation and starvation. In this paper, we established a protocol for screening agonistic molecules towards ecdysone receptor of insect cells line S2 (Diptera) and Sf9 (Lepidoptera), transfected with the reporter plasmid ere.b.act.luc. Therefore, we set dose-response curves with the ecdysteroid 20-hydroxyecdysone, the phytoecdysteroid ponasterone-A, and tebufenozide, a pesticide belonging to the class of diacylhydrazines. In both cell lines, the median effective concentration values on reporter gene induction (EC50) of ponasterone-A was the smallest, meaning the most active agonist molecule. In Sf9 cells, tebufenozide had as smaller EC50 than 20-hydroxyecdysone, indicating the high agonistic capability and lepidopteran specificity. The protocol established in this study can be useful for a quick screening and rational research of site-specific pesticides.(AU)

O receptor de ecdisona, naturalmente ativado por hormônios esteroidais, é uma proteína-chave nos processos de muda e reprodução de insetos. A ativação artificial desse receptor por meio de pesticidas específicos induz um processo de ecdise anômala, levando o inseto à morte por dessecação e inanição. Neste trabalho, foi estabelecido um protocolo para a triagem de moléculas agonistas em relação ao receptor de ecdisona nas linhagens celulares responsivas S2 (Diptera) e Sf9 (Lepidoptera), transfectadas com o plasmídeo repórter ere.b.act.luc. Para tanto, curvas de dose-resposta foram estabelecidas com o ecdisteroide 20-hidroxiecdisona, o fitoecdisteroide ponasterona-A e tebufenozida, um pesticida pertencente à classe das diacilhidrazinas. Em ambas linhagens celulares, os valores médios de concentração efetiva para indução gênica (EC50) ponasterona-A foram menores, significando que este é o agonista mais potente. Em células Sf9, a tebufenozida apresentou EC50 menor que a 20-hidroxiecdisona, indicando uma alta atividade agonista e especificidade deste inseticida a lepidópteros. O protocolo estabelecido neste trabalho pode ser utilizado para uma rápida triagem e busca racional de pesticidas de alvo bioquímico específico.(AU)
Descritores: Plasmídeos
Muda
Insetos
-Praguicidas
Ecdisterona
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: biblio-1022489
Autor: Ilicheva, Nadya V; Travina, Aleksandra O; Voronin, Alexey P; Podgornaya, Olga I.
Título: Development and characterization of polyclonal antibodies against the linker region of the telomere-binding protein TRF2
Fonte: Electron. j. biotechnol;32:1-5, Mar. 2018. ilus.
Idioma: en.
Projeto: RFBR; . RSF.
Resumo: Background: TRF2 (telomeric repeat binding factor 2) is an essential component of the telomere-binding protein complex shelterin. TRF2 induces the formation of a special structure of telomeric DNA and counteracts activation of DNA damage-response pathways telomeres. TRF2 has a poorly characterized linker region (udTRF2) between its homodimerization and DNA-binding domains. Some lines of evidence have shown that this region could be involved in TRF2 interaction with nuclear lamina. Results: In this study, the fragment of the TERF2 gene encoding udTRF2 domain of telomere-binding protein TRF2 was produced by PCR and cloned into the pET32a vector. The resulting plasmid pET32a-udTRF2 was used for the expression of the recombinant udTRF2 in E. coli RosettaBlue (DE3). The protein was isolated and purified using ammonium sulfate precipitation followed by ion-exchange chromatography. The purified recombinant protein udTRF2 was injected into guinea pigs to generate polyclonal antibodies. The ability of anti-udTRF2 antibodies to bind endogenous TRF2 in human skin fibroblasts was tested by western blotting and immunofluorescent staining. Conclusions: In this study, the recombinant protein udTRF2 and antibodies to it were generated. Both protein and antibodies will provide a useful tool for investigation of the functions of the udTRF2 domain and its role in the interaction between TRF2 and nuclear lamina.
Descritores: Proteína 2 de Ligação a Repetições Teloméricas/metabolismo
Anticorpos/metabolismo
-Plasmídeos
Proteínas Recombinantes/metabolismo
Imuno-Histoquímica
Western Blotting
Cromossomos
Clonagem Molecular
Lâmina Nuclear
Proteína 2 de Ligação a Repetições Teloméricas/genética
Imunoprecipitação
Proteínas de Ligação a DNA/metabolismo
Escherichia coli/metabolismo
Anticorpos/isolamento & purificação
Formação de Anticorpos
Nucleoproteínas
Limites: Animais
Cobaias
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1021652
Autor: Wu, Yan; Li, Tao; Cao, Qinghua; Li, Xuedan; Zhang, Yizheng; Tan, Xuemei.
Título: RecET recombination system driving chromosomal target gene replacement in Zymomonas mobilis
Fonte: Electron. j. biotechnol;30:118-124, nov. 2017. tab, ilus, graf.
Idioma: en.
Projeto: National Key Technology R&D Program.
Resumo: Background: Zymomonas mobilis is a Gram-negative microaerophilic bacterium with excellent ethanol-producing capabilities. The RecET recombination system provides an efficient tool for direct targeting of genes in the bacterial chromosome by PCR fragments. Results: The plasmids pSUZM2a-RecET and pSUZM2a-RecE588T were first developed to co-express RecE or RecE588 and RecT for homologous recombination. Thereafter, the PCR fragments of the tetracycline resistance marker gene flanked by 60 bp of adhA (alcohol dehydrogenase I) or adhB (alcohol dehydrogenase II) homologous sequences were electroporated directly into ZM4 cells harboring pSUZM2a-RecET or pSUZM2a-RecE588T. Both adhA and adhB were replaced by the tetracycline resistance gene in ZM4, yielding two mutant strains, Z. mobilis ZM4 ΔadhA and Z. mobilis ZM4 ΔadhB. These two mutants showed varying extent of reduction in ethanol production, biomass generation, and glucose metabolism. Furthermore, enzyme activity of alcohol dehydrogenase II in Z. mobilis ZM4 ΔadhB exhibited a significant reduction compared to that of wild-type ZM4. Conclusion: This approach provided a simple and useful method for introducing mutations and heterologous genes in the Z. mobilis genome.
Descritores: Zymomonas/genética
Recombinação Homóloga
-Plasmídeos
Recombinação Genética
Álcool Desidrogenase/metabolismo
Zymomonas/enzimologia
Eletroporação
Etanol/metabolismo
Técnicas de Inativação de Genes
Mutação
Responsável: CL1.1 - Biblioteca Central


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Vicente, Ana Carolina Paulo
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Id: biblio-976242
Autor: Morgado, Sergio Mascarenhas; Vicente, Ana Carolina Paulo.
Título: Exploring tRNA gene cluster in archaea
Fonte: Mem. Inst. Oswaldo Cruz;114:e180348, 2019. tab, graf.
Idioma: en.
Projeto: CAPES; . CNPq.
Resumo: BACKGROUND Shared traits between prokaryotes and eukaryotes are helpful in the understanding of the tree of life evolution. In bacteria and eukaryotes, it has been shown a particular organisation of tRNA genes as clusters, but this trait has not been explored in the archaea domain. OBJECTIVE Explore the occurrence of tRNA gene clusters in archaea. METHODS In-silico analyses of complete and draft archaeal genomes based on tRNA gene isotype and synteny, tRNA gene cluster content and mobilome elements. FINDINGS We demonstrated the prevalence of tRNA gene clusters in archaea. tRNA gene clusters, composed of archaeal-type tRNAs, were identified in two Archaea class, Halobacteria and Methanobacteria from Euryarchaeota supergroup. Genomic analyses also revealed evidence of the association between tRNA gene clusters to mobile genetic elements and intra-domain horizontal gene transfer. MAIN CONCLUSIONS tRNA gene cluster occurs in the three domains of life, suggesting a role of this type of tRNA gene organisation in the biology of the living organisms.
Descritores: RNA de Transferência/análise
Archaea/classificação
Euryarchaeota/virologia
-Plasmídeos
Haloarcula
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-889216
Autor: Yang, Xue-Jing; Wang, Sai; Cao, Jun-Min; Hou, Jia-Hui.
Título: Complete genome sequence of human pathogen Kosakonia cowanii type strain 888-76T
Fonte: Braz. j. microbiol;49(1):16-17, Jan.-Mar. 2018.
Idioma: en.
Projeto: Science and Technology Program of Zhejiang Province; . Zhejiang Provincial Medical and Health Science.
Resumo: ABSTRACT Kosakonia cowanii type strain 888-76T is a human pathogen which was originally isolated from blood as NIH group 42. In this study, we report the complete genome sequence of K. cowanii 888-76T. 888-76T has 1 chromosome and 2 plasmids with a total genome size of 4,857,567 bp and C+G 56.15%. This genome sequence will not only help us to understand the virulence features of K. cowanii 888-76T but also provide us the useful information for the study of evolution of Kosakonia genus.
Descritores: Genoma Bacteriano
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/genética
Infecções por Enterobacteriaceae/microbiologia
-Filogenia
Plasmídeos/genética
Composição de Bases
DNA Bacteriano/genética
Dados de Sequência Molecular
Sequência de Bases
Enterobacteriaceae/classificação
Limites: Seres Humanos
Responsável: BR1.1 - BIREME



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