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Texto completo SciELO Brasil
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Id: biblio-954055
Autor: Kajbafzadeh, Abdol-Mohammad; Sabetkish, Shabnam; Sabetkish, Nastaran.
Título: The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
Fonte: Int. braz. j. urol;44(3):608-616, May-June 2018. tab, graf.
Idioma: en.
Projeto: Tehran University of Medical Sciences.
Resumo: ABSTRACT Purpose: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. Materials and Methods: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was induced on the right kidney at gestational day 11. The same procedure was performed in eight non-pregnant rats as control group. Three months after delivery, right ne- phrectomy was performed in order to evaluate the injured kidney. The fresh perfused kidneys were stained with anti-GFP antibody. Polymerase chain reaction (PCR) assay was also performed for the Y chromosome detection. Cell culture was performed to detect the GFP-positive cells. Technetium-99m-DMSA renal scan and single-photon emission computed tomography (SPECT) were performed after renal damage induction and 3 months later to evaluate the improvement of renal integrity. Results: The presence of FSCs was confirmed by immune histochemical staining as well as immunofluorescent imaging of the damaged part. Gradient PCR of female rat purified DNA demonstrated the presence of Y-chromosome in the damaged maternal kidney. Moreover, the culture of kidney cells showed GPF- positive cells by immuno- fluorescence microscopy. The acute renal scar was repaired and the integrity of dam- aged kidney reached to near normal levels in experimental group as shown in DMSA scan. However, no significant improvement was observed in control group. Conclusion: FSC seems to be the main mechanism in repairing of the maternal renal injury during pregnancy as indicated by Y chromosome and GFP-positive cells in the sub-cultured medium.
Descritores: Cicatrização/fisiologia
Quimerismo
Células-Tronco Fetais/fisiologia
Nefropatias/fisiopatologia
Troca Materno-Fetal/fisiologia
-Fatores de Tempo
Cromossomo Y
Imuno-Histoquímica
Tomografia Computadorizada de Emissão de Fóton Único
Células Cultivadas
Reação em Cadeia da Polimerase
Imunofluorescência
Ratos Sprague-Dawley
Compostos Radiofarmacêuticos
Ácido Dimercaptossuccínico Tecnécio Tc 99m
Modelos Animais de Doenças
Nefropatias/patologia
Nefropatias/diagnóstico por imagem
Limites: Animais
Masculino
Feminino
Gravidez
Responsável: BR1.1 - BIREME


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Id: biblio-1102154
Autor: Vernaza, Alejandro; Gutiérrez, Yira.
Título: Evaluación De Quimerismo En Pacientes Con Injerto De Células Progenitoras Hematopoyéticas En Panamá Del 2000­20 / Evaluation of Chimerism in Patients with Graft Hematopoietic Progenitor Cells in Panama from 2000­2018
Fonte: Rev. méd. Panamá;39(1):8-13, 2019.
Idioma: es.
Resumo: Introducción: El Injerto de células progenitoras hematopoy éticas (ICPH) es actualmente un trata­miento para diferentes desórdenes hematológicos malignos y no malignos. El análisis del quimeris­mo post ICPH, y la cuantificación de cada población celular, deben ser monitoreados. El presente trabajo tiene como objetivo: el evaluar los res ultados de quimerismo completo y mixto en sangre periférica del receptor pos trasplante obtenidos por método cualitativo y cuantitativo del año 2000 al 2018. Material y método: El presente es un estudio descriptivo, observacional, transversal de dos mé­ todos de quimerismo efectuados a receptores y donantes de ICPH alogénico. Resultados: De los 79 pacientes estudiados por el método cualitativo: 65 (82.2%) resultaron con qui­ merismo completo y 14 (17.7%) con quimerismo mixto. No fue posible cuantificar por este método el % de células del donante y del receptor.Conclusión: El método cuantitativo es un método exacto, que determina el % de células del receptor y del donante presentes en la muestra. Con este método se evalúan un mayor número de marcadores genéticos que con el método cualitativo, y se obtienen un mayor número de loci informativos del quimerismo al compararlo con el método cualitativo.

Introduction: Hematopoietic progenitor cell grafting (ICPH) is currently a treatment for different ma­ lignant and non­malignant hematological disorders. The analy sis of post­ICPH chimerism, and the quantification of each cell population, should be monitored. The present work has as objective: to evaluate the results of complete and mixed chimerism in peripheral blood of the post­trans plant reci­pient obtained by qualitative and quantitative method from the year 2000 to 2018. Material and method: The present is a descriptive, observational, cross­sectional study of two met­ hods of chimerism performed on allogeneic ICPH recipients and donors . Results: Of the 79 patients studied by the qualitative method: 65 (82.2%) resulted with complete chi­ merism and 14 (17.7%) with mixed chimerism. It was not possible to quantify by this method the% of donor and recipient cells. Conclusion: The quantitative method is an exact method, which determi­nes the% of recipient and donor cells present in the sample. With this method, a greater number of genetic markers are evaluated than in the qualitative method, and a greater number of information loci of chimerism are obtained than with the qualitative method.
Descritores: Transplante de Células-Tronco Hematopoéticas/estatística & dados numéricos
Quimerismo/classificação
Quimerismo/efeitos dos fármacos
-Doenças Hematológicas
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo Comparativo
Responsável: PA30.1 - BINASA - Biblioteca Nacional de Salud


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Texto completo SciELO Brasil
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Id: biblio-886829
Autor: SILVA, MONIQUE O M; ARMADA, JORGE LUÍS A DE; VERONA, CARLOS EDUARDO S; HELIODORO, GABRIELA; NOGUEIRA, DENISE M.
Título: Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates)
Fonte: An. acad. bras. ciênc;89(4):2793-2804, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A fragment of approximately 200bp of the SRY gene was amplified in seven females with normal external genitalia. The percentage of 32% of chimeric individuals detected in the present study is similar to that observed for pure specimens of Callithrix. These data suggests that hybridization probably does not interfere with the occurrence of twin gestation, nor of chimerism. Although cytogenetics is the main tool to identify the two cell lineages present in cases of chimerism, the amplification of the SRY gene by PCR has proved to be more efficient to identify the Y chromosome in cases of chimeric female marmoset.
Descritores: Callithrix/genética
Quimerismo
-Reação em Cadeia da Polimerase
Citogenética
Genes sry
Cariótipo
Tamanho da Ninhada de Vivíparos/genética
Limites: Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Id: lil-743642
Autor: Schettini, Juliana Araújo de Carvalho; Torres, Leuridan Cavalcante.
Título: A placenta não é o limite entre o compartimento materno-fetal / The placental is not the boundary between the maternal and fetal compartment
Fonte: Femina;42(5):217-218, set.-out. 2014.
Idioma: pt.
Descritores: Placenta/citologia
Placenta/embriologia
DNA
-Trofoblastos
Quimerismo/embriologia
Limites: Feminino
Gravidez
Tipo de Publ: Editorial
Responsável: BR1365.1 - Biblioteca Biomédica A - CB/A


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Texto completo SciELO Costa Rica
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Id: lil-637483
Autor: Quirós Alpízar, José Luis; Alpízar Miranda, Kattia E.
Título: Quimerismo genético un nuevo paradigma para la medicina legal / Genetic chimerism a new paradigm for legal medicine
Fonte: Med. leg. Costa Rica;26(2):73-78, sep. 2009.
Idioma: es.
Resumo: El quimerismo genético un concepto no bien esclarecido en la comunidad científica, aún confundido con mosaicismo. En la presente revisión pretendemos definirlo con un enfoque legal y ejemplificarlo con una serie de casos descritos en la literatura médica, los cuales nos orientan a la diferencia clara entre mosaicismo y quimerismo. Dentro de esta serie separamos los casos que se relacionan con hermafroditismo y los no relacionados. Además, hacemos referencia a un nuevo concepto que genera polémica en la investigación, el microquimerismo, relacionado con transfusiones sanguíneas, transplantes y la génesis de las enfermedades autoinmunes.

Genetic chimerism is a not well defined concept among the scientific community; it is still confused with mosaicism. In the following review we intend to define the concept with a legal point of view and exemplify it with a series of cases described in medical texts. The cases lead us to find the clear difference between mosaicism and chimerism. Contained in these series of cases we have separated the ones related with hemaphroditism from the ones that are not related. We also make reference to a new concept that generates controversy in the investigation; microchimerism, which is related with blood transfusions, transplants and genesis of autoimmune diseases..
Descritores: Transtornos do Desenvolvimento Sexual
Transtornos dos Cromossomos Sexuais/patologia
Quimerismo
Jurisprudência
Mosaicismo
-Costa Rica
Medicina Legal
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Id: lil-785543
Autor: Bodni, Rosa Ana.
Título: Microquimerismo en dermtología / Microchimerism in dermatology
Fonte: Dermatol. argent;19(1):48-52, 2013. graf.
Idioma: es.
Resumo: Se denomina microquimerismo a la presencia en un organismo de una pequeña población de células o ADN que proviene de otro individuo genéticamente diferente. La causa más común de microquimerismo adquirido en forma natural es el tráfico bidireccional de células que se produce durante el embarazo entre la madre y el feto. Puede tener efectos benéficos o nocivospara el huésped, dependiendo de múltiples factores. Ha sido implicado en la patogenia de enfermedades autoinmunes como la esclerodermia, el lupus eritematoso sistémico, el lupusneonatal y la dermatomiositis, en la reparación de tejidos y el cáncer...
Descritores: DNA
Gravidez
Quimerismo/embriologia
-Doenças Autoimunes
Feto
Limites: Humanos
Tipo de Publ: Revisão
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Texto completo SciELO Costa Rica
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Id: lil-753634
Autor: Jiménez, Isabel Arce; Cubillo Suarez, Walter; Hütt Centeno, Erika; Rivera Arenas, Alexander; Rodríguez Mena, Denisse.
Título: Implicaciones médico legales del quimerismo / Medicolegal implications of chimerstry
Fonte: Med. leg. Costa Rica;32(1):102-108, ene.-mar. 2015. ilus.
Idioma: es.
Resumo: Los recientes avances en tecnologías biomédicas proporcionan herramientas a la Medicina Legal para el esclarecimiento de casos complejos y ejercer justicia basada en evidencia científica. ¿Pero qué ocurre cuando se imponen obstáculos como el quimerismo que podrían llevar a decisiones equivocadas? A pesar de que este fenómeno se creía casi inexistente, se han reportado interesantes casos, que han puesto a prueba la utilidad de las pruebas de ADN. El quimerismo se define como la presencia de líneas celulares con distinto material genético proveniente de diferentes orígenes en un único cuerpo. Esto tiene grandes implicaciones medico legales, principalmente en la investigación criminal. Por ejemplo en una escena de crimen, se pueden encontrar muestras de tejidos de un mismo individuo pero estas podrían tener ADN distinto si se trata de un individuo quimérico llevando a una mala interpretación de la información. También cabe resaltar las implicaciones del quimerismo en las pruebas de paternidad, ya que este fenómeno puede ocasionar falsos negativos en estas pruebas y por lo tanto un diagnóstico incierto de paternidad. El objetivo de esta revisión es describir las diferentes implicaciones médico legales del quimerismo y proponer posibles soluciones a los conflictos que podrían presentarse ante tales casos.

Recent advances in biomedical technologies are able to clarify today's complex cases that are faced by Legal Medicine; allowing this branch of medicine to exercise justice based in scientific evidence. But what happens when an obstacle such as chimerism is present and may lead to false decisions? Although this phenomenon was thought to be inexistent, interesting cases have been reported. Chimerism is defined as the presence of different cell linings in a unique organism. This phenomenon has important implication in Legal Medicine, especially in criminal investigation. For example, in a crime scene the samples gathered may present different DNA and lead to misinterpretation of the information. On the other hand, paternity tests are also implicated in the presence of a chimera since it may originate a false negative result. The purpose of this review is to describe different Legal Medicine's implications linked to chimerism and propose possible solutions to the conflicts that may arouse from a case of a chimera.
Descritores: Quimerismo
DNA
Medicina Legal
Mosaicismo
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Texto completo SciELO Brasil
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Id: lil-725670
Autor: Merzoni, Jóice; Ewald, Gisele Menezes; Paz, Alessandra Aparecida; Daudt, Liane Esteves; Jobim, Luiz Fernando Job.
Título: Quantification of mixed chimerism allows early therapeutic interventions
Fonte: Rev. bras. hematol. hemoter;36(5):369-372, Sep-Oct/2014. tab.
Idioma: en.
Resumo: Hematopoietic stem cell transplantation is the curative option for patients with myelodysplastic syndrome; however, it requires a long post-transplantation follow-up. A 53-year-old woman with a diagnosis of myelodysplastic syndrome underwent related donor allogeneic hematopoietic stem cell transplantation in July 2006. Three months after transplantation, a comparative short tandem repeat analysis between donor and recipient revealed full chimerism, indicating complete, healthy bone marrow reconstitution. Three years and ten months after hematopoietic stem cell transplantation, the patient developed leukopenia and thrombocytopenia. Another short tandem repeat analysis was carried out which showed mixed chimerism (52.62%), indicating relapsed disease. A donor lymphocyte infusion was administered. The purpose of donor lymphocyte infusion is to induce a graft-versus-leukemia effect; in fact, this donor's lymphocyte infusion induced full chimerism. Successive short tandem repeat analyses were performed as part of post-transplantation follow-up, and in July 2010, one such analysis again showed mixed chimerism (64.25%). Based on this finding, a second donor lymphocyte infusion was administered, but failed to eradicate the disease. In September 2011, the patient presented with relapsed disease, and a second related donor allogeneic hematopoietic stem cell transplantation was performed. Subsequent short tandem repeat analyses revealed full chimerism, indicating complete bone marrow reconstitution. We conclude that quantitative detection of mixed chimerism is an important diagnostic tool that can guide early therapeutic intervention...
Descritores: Transplante de Medula Óssea
Quimerismo
Doenças Mieloproliferativas-Mielodisplásicas
Sequências de Repetição em Tandem
Limites: Humanos
Tipo de Publ: Relatos de Casos
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-632172
Autor: Ruiz-Argüelles, Guillermo J; Bordes-Aznar, Javier; Díaz-Caballero, Norma; Ruiz-Delgado, Guillermo J.
Título: La importancia del quimerismo en medicina / Importance of Chimerism in Medicine
Fonte: Gac. méd. Méx;140(5):573-575, sep.-oct. 2004. ilus.
Idioma: es.
Descritores: Quimerismo
Medicina Clínica
Responsável: MX1.1 - CENIDSP - Centro de Información para Decisiones en Salud Pública


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Id: lil-613044
Autor: Tabares, Juan Hember; Fierro, Carlos Humberto; Pulido, Paola Del Pilar; Ossa Reyes, Humberto.
Título: Cariotipo del tití gris (Saguinus leucopus): similitudes con el cariotipo humano
Fonte: NOVA publ. cient;6(10):116-125, jul.-dic. 2008. ilus.
Idioma: es.
Resumo: El tití gris (Saguinus leucopus) es un primate endémico de Colombia cuyo cariotipo se describe en el presente estudio a partir de una pareja de individuos ubicados en el Centro de Rehabilitación de Fauna Silvestre del Oriente de Caldas, Colombia. Las muestras de sangre fueron recolectadas de la vena femoral y anticoaguladas con heparina de sodio. Los cromosomas se obtuvieron por el método clásico de cultivo de linfocitos y bandeamiento Q y G Los individuos presentan 46 cromosomas (2n = 46: 30Bi, 14A); cromosomas sexuales XX en la hembra y XY en el macho (quimerismo 46,XX/46,XY en este último). Se propone un ideograma para el cariotipo del Tití Gris. Se observan amplias semejanzas en los cromosomas X y 5 de S. leucopus con los cromosomas X y 19 humanos, respectivamente. Otras similitudes parciales se evidenciaron entre los cromosomas 1 de ambas especies, 2 y 14 de S. leucopus con el 7 humano. La comparación del tamaño de regiones exónicas de dos genes de S. leucopus y Homo sapiens no arrojó diferencia.
Descritores: Quimerismo
Cromossomos
Cariotipagem
Primatas
Saguinus
Responsável: CO242.1 - Biblioteca



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