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Id: biblio-1053211
Autor: Pokorska, Joanna; Piestrzynska-Kajtoch, Agata; Kulaj, Dominika; Ochrem, Andrzej; Radko, Anna.
Título: Polymorphism of bovine lipocalin-2 gene and its impact on milk production traits and mastitis in Holstein Friesian cattle
Fonte: Electron. j. biotechnol;40:17-21, July. 2019. tab, ilus.
Idioma: en.
Projeto: Ministry of Science and Higher Education in Poland.
Resumo: Background: Mastitis is one of the most serious diseases of dairy cattle, causing substantial financial losses. While predisposition to reduced somatic cell count in milk has been considered for in cattle breeding programs as the key indicator of udder health status, scientists are seeking genetic markers of innate immune response, which could be helpful in selecting cows with improved immunity to mastitis. Lipocalin-2 (LCN2) is a protein involved in the response of the immune system by eliminating iron ions which are necessary for the growth of pathogenic bacteria, so LCN2 may be considered as a natural bacteriostatic agent and could become a marker of infection. Results: A total of five SNPs were identified in LCN2 gene (one in the promoter, three in exon 1, and one in intron 1). A single haplotype block was identified. The locus g.98793763GNC was found to have a significant impact on protein levels in milk, and alleles of this locus were identified to have a significant positive dominance effect on this trait. None of the four analysed loci had a statistically significant impact on the milk yield, fat levels in milk or the somatic cell score. LCN-2 gene had no significant impact on the incidence of mastitis in the cows. Conclusions: Although the identified SNPs were not found to have any impact on the somatic cell count or the incidence of mastitis in cows, it seems that further research is necessary, covering a larger population of cattle, to confirm the association between lipocalin-2 and milk production traits and mastitis.
Descritores: Polimorfismo Genético
Leite/imunologia
Lipocalina-2/genética
Mastite Bovina/genética
-Haplótipos
Cruzamento
Polimorfismo de Nucleotídeo Único
Alelos
Lipocalina-2/química
Glândulas Mamárias Animais
Mastite Bovina/imunologia
Limites: Animais
Bovinos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-974226
Autor: Zahirnia, Amir Hossein; Bordbar, Ali; Ebrahimi, Sahar; Spotin, Adel; Mohammadi, Somayeh; Ghafari, Seyedeh Maryam; Ahmadvand, Setareh; Jabbari, Negar; Esmaeili Rastaghi, Ahmad Reza; Parvizi, Parviz.
Título: Predominance of Leishmania major and rare occurrence of Leishmania tropica with haplotype variability at the center of Iran
Fonte: Braz. j. infect. dis;22(4):278-287, July-Aug. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Background Leishmania major is a causative agent of zoonotic cutaneous leishmaniasis in the center of Iran, Abarkouh district. Molecular characterization and precise incrimination of Leishmania species was carried out to perform controlling measurements and to design treatment programs for zoonotic cutaneous leishmaniasis. Methods All smears isolated from ulcers of suspected patients were examined under a light microscope and graded for amastigotes frequency. Extraction of DNA, PCR, RFLP and sequencing of ITS-rDNA genotype were done to increase the efficacy of Leishmania parasites identification at their species-specific level and to detect any Leishmania infections within. Results Humans were found to be infected with L. major with high infection frequency and also Leishmania tropica was identified with low occurrence for the first time as non-native species using molecular analyses. The rates of infections was considerable with microscopic observation (n= 65, 73%) out of 89 smears prepared from suspected patients. Molecular analyses showed that the density of L. major was significantly higher (n= 48, 53.93%) than L. tropica (n= 4, 4.49%) (Mann-Whitney U test: p< 0.05) and two samples (2.25%) remained ambiguous after several sequencing. L. major did not have diversity with two common haplotypes but L. tropica were found to exhibit high diversity with three novel haplotypes. Conclusion L. major was considered the causative agent of leishmaniasis in the region, but the identification of a non-native L. tropica revealed the importance of further isolation of Leishmania parasites following molecular analyses and confirmation, and also revealed the importance of further isolation of Leishmania parasites from patients of the field areas who do not have easily access to health care centers for specialized treatment strategies.
Descritores: Leishmania tropica/genética
Leishmaniose Cutânea/parasitologia
Leishmania major/genética
-População Rural
Haplótipos
Polimorfismo de Fragmento de Restrição
Leishmania tropica/isolamento & purificação
Leishmania tropica/ultraestrutura
Reação em Cadeia da Polimerase
DNA de Protozoário/isolamento & purificação
DNA de Protozoário/genética
Leishmaniose Cutânea/patologia
Leishmaniose Cutânea/epidemiologia
Leishmania major/isolamento & purificação
Doenças Endêmicas
Irã (Geográfico)
Limites: Humanos
Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Chianca, Tânia Couto Machado
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Id: lil-761693
Autor: Guedes, Helisamara Mota; Souza, Kesia Meiriele; Lima, Patrícia de Oliveira; Martins, José Carlos Amado; Chianca, Tânia Couto Machado.
Título: Relationship between complaints presented by emergency patients and the final outcome / Relação entre queixas apresentadas por pacientes na urgência e o desfecho final / Relación entre las quejas presentadas por pacientes en la urgencia y el desenlace final
Fonte: Rev. latinoam. enferm. (Online);23(4):587-594, July-Aug. 2015. tab.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de Minas Gerais.
Resumo: AbstractObjective: to relate complaints presented by emergency room patients, classified using the Manchester Triage System, with the final outcome (discharge/death/transfer).Methods: prospective cohort study, involving 509 patients who were admitted to the emergency room and remained there for more than 24 hours after admission, being monitored to the final outcome. Data were analyzed with a statistical program using descriptive and analytical statistics.Results: the mean age of the patients was 59.1 years and 59.3% were male. The main complaints were unwell adult (130 - 22.5%), shortness of breath in adults (81 - 14.0%), abdominal pain in adults (58 - 10.0%) and behaving strangely (34 - 5.9%), with 87% of the patients being discharged. More deaths were found in the patients classified in the severe colors, with 42.8% classified as red, 17.0% as orange and 8.9% as yellow. Among the patients classified as green, 9.6% died.Conclusion: in the various colors of the Manchester Triage System, death prevailed in patients that presented the complaints of unwell adult, shortness of breath, head injury, major trauma, diarrhea and vomiting. The higher the clinical priority the greater the prevalence of death.

ResumoObjetivo:relacionar queixas apresentadas pelos pacientes classificados pelo Sistema de Triagem de Manchester em um pronto-socorro com o desfecho final (alta/óbito/transferência).Métodos:estudo de coorte prospectivo, realizado com 509 pacientes que deram entrada no pronto-socorro e que nele permaneceram por mais de 24 horas após a admissão, sendo acompanhados até o desfecho final. Os dados foram digitados e analisados com estatística descritiva e analítica em um pacote estatístico.Resultados:entre os pacientes, 59,3% eram do sexo masculino, com idade média de 59,1 anos. As queixas principais eram de mal-estar no adulto (130-22,5%), dispneia em adulto (81-14,0%), dor abdominal em adulto (58-10,0%), alterações de comportamento (34-5,9%), sendo que, desses, 87% recebeu alta. Foram encontrados mais óbitos nos pacientes classificados nas cores mais graves, sendo 42,8% classificados como vermelho, 17,0% laranja e 8,9% como amarelo. Entre os pacientes classificados como verde, 9,6% evoluiu para óbito.Conclusão:nas diversas cores do Sistema de Triagem Manchester, o óbito prevaleceu nos pacientes que apresentaram a queixa de mal-estar no adulto, dispneia, sofreram trauma craniano, trauma maior, diarreia e vômito. Quanto maior a prioridade clínica maior a prevalência de óbito.

ResumenObjetivo:relacionar las quejas presentadas por los pacientes clasificados por el Sistema de Clasificación de Manchester, en un servicio de urgencia, con el desenlace final (alta/muerte/ transferencia).Métodos:estudio de cohorte prospectiva, realizado con 509 pacientes que dieron entrada en el servicio de urgencia y que en él permanecieron por más de 24 horas después de la admisión, siendo seguidos hasta el desenlace final. Los datos fueron introducidos y analizados con estadística descriptiva y analítica, en un programa estadístico.Resultados:entre los pacientes, 59,3% eran del sexo masculino, con edad promedio de 59,1 años. Las quejas principales eran de malestar en adulto (130-22,5%), disnea en adulto (81-14,0%), dolor abdominal en adulto (58- 10,0%), alteraciones de comportamiento (34-5,9%), siendo que, de estos, 87% recibió alta. Fueron encontradas más muertes entre los pacientes clasificados con los colores más graves, siendo 42,8% clasificados como rojo, 17,0% naranja y 8,9% como amarillo. Entre los pacientes clasificados como verde, 9,6% evolucionó para la muerte.Conclusión:en los diversos colores del Sistema de Clasificación Manchester, la muerte prevaleció en los pacientes que presentaron la queja de malestar en adulto, disnea, sufrieron trauma craniano, trauma mayor, diarrea y vómito. Cuanto mayor es la prioridad clínica mayor es la prevalencia de la muerte.
Descritores: Antígenos HLA-B
Haplótipos/imunologia
Transplante de Células-Tronco Hematopoéticas
Leucemia-Linfoma Linfoblástico de Células Precursoras
Fator de Necrose Tumoral alfa
-Aloenxertos
Intervalo Livre de Doença
Doença Enxerto-Hospedeiro/genética
Doença Enxerto-Hospedeiro/imunologia
Doença Enxerto-Hospedeiro/mortalidade
Antígenos HLA-B/genética
Antígenos HLA-B/imunologia
Leucemia-Linfoma Linfoblástico de Células Precursoras/genética
Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia
Leucemia-Linfoma Linfoblástico de Células Precursoras/mortalidade
Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia
Irmãos
Taxa de Sobrevida
Doadores de Tecidos
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
Limites: Adolescente
Adulto
Criança
Pré-Escolar
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Ensaio Clínico
Estudo Multicêntrico
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1011172
Autor: Abu-Hassan, Diala W; Alhouri, Abdullah N; Altork, Nadera A; Shkoukani, Zakaria W; Altamimi, Tamer Salhab; Alqaisi, Omar M; Mustafa, Baha.
Título: MTHFR gene polymorphisms in hypothyroidism and hyperthyroidism among Jordanian females
Fonte: Arch. endocrinol. metab. (Online);63(3):280-287, May-June 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective Methylenetetrahydrofolate reductase (MTHFR) is involved in DNA methylation that is associated with autoimmune pathology. We investigated the association between MTHFR genetic polymorphisms at g.677C>T and g.1298A>C and their haplotypes, and the risk of thyroid dysfunction among Jordanian females. Subjects and methods A case-control study involving 98 hypothyroidism cases, 66 hyperthyroidism cases and 100 controls was conducted. Polymerase chain reaction/restriction fragment length polymorphism technique was performed to determine genotypes. Statistical analysis using SPSS software was performed. Results Genetic analysis showed a significant difference in genotype frequency of g.1298A>C between cases, and controls [hypothyroidism: AA (45.9%), AC (37.8%), CC (16.3%); hyperthyroidism: AA (9.1%), AC (69.7%), CC (21.2%); controls: AA (37.8%), AC (29.6%), CC (32.7%); CChypo vs. AAhypo: 2.55, 95% CI: (1.18-5.52); OR at least on Chypo: 1.79, 95% CI: (1.07-2.99)]; CChyper vs. AAhyper: 4.01, 95% CI: (1.79-9.01); OR at least on Chyper: 0.18, 95% CI: (0.07-0.48)]. There was no significant difference in genotype frequency of g.677C>T between cases and controls [hypothyroidism: CC (50.0%), CT (32.7%), TT (17.3%); hyperthyroidism: CC (77.3%), CT (15.2%), TT (7.6%); controls: CC (55.6%), CT (32.3%), TT (12.1%)]. There was a significant difference of MTHFR haplotypes among hypothyroidism cases and controls. TA and CC had a lower hypothyroidism risk whereas; TC showed a higher risk. Conclusions g.1298A>C genetic polymorphism of MTHFR may modulate the risk of thyroid disease. CC, TA, and TC haplotypes affect the risk of hypothyroidism. Larger samples should be included in the future to verify the role of MTHFR polymorphisms in thyroid diseases.
Descritores: Polimorfismo de Fragmento de Restrição/genética
Polimorfismo de Nucleotídeo Único/genética
Metilenotetra-Hidrofolato Redutase (NADPH2)/genética
Hipertireoidismo/genética
Hipotireoidismo/genética
-Haplótipos
Estudos de Casos e Controles
Reação em Cadeia da Polimerase
Fatores de Risco
Metilação de DNA
Predisposição Genética para Doença
Alelos
Genótipo
Jordânia
Limites: Humanos
Feminino
Adulto
Pessoa de Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: lil-677719
Autor: Salazar Granara, Alberto; Sandoval Sandoval, José; Mendizábal Arbocco, Rafael; Kikushima Tukiuda, Francisco; Madsen, Hans O; Garred, Peter; Fujita Alarcón, Ricardo.
Título: Variantes del gen Mannose Binding Lectin (MBL) en pobladores amazónicos de Andoas - Loreto y su posible implicancia en la salud / Variants of gene MBL in native amazonians from Andoas - Loreto, and its possible implications in health
Fonte: Horiz. méd. (Impresa);6(1):11-16, jun. 2006. tab.
Idioma: es.
Resumo: El gen MBL (Mannose Binding Lectin) codifica una proteína de la inmunidad innata que activa el sistema del complemento, así como recluta macrófagos y quimioquinas proinflamatorias. Estudios realizados en otras latitudes han asociado susceptibilidad o resistencia a enfermedades infecciosas, autoinmunes y cardiovasculares con alelos deficientes de MBL. Sin embargo, muchos estudios no son concluyentes debido a la rareza de estos alelos en las poblaciones estudiadas. Previamente hemos demostrado que en las islas del Lago Titicaca, el alelo deficiente B tiene la más alta prevalencia del mundo. Por ello, queremos corroborar su presencia en zonas más cálidas que son más propicias a enfermedades infecciosas. Para ello se analizó el genotipo de 94 individuos procedentes de la región amazónica de Andoas-Loreto. Aunque en menor proporción que en las islas del Lago Titicaca, la frecuencia de la variante deficiente B en Andoas es más alta que en otras poblaciones del mundo. Esta frecuencia y su gran exposición a enfermedades tropicales, hacen de Andoas una población interesante para estudiar el rol de las variantes de MBL en el desarrollo de las mismas.

The gene MBL codifies for a protein that has a role in innate immunity by activating the complement system as well as recruiting macrophages and proinflammatory chemokines. Studies performed around the world have associated susceptibility/resistance to infectious, autoimmune and cardiovascular diseases, with deficient alleles of MBL. However, many of these studies remain inconclusive because of the rarity of these alleles. We have previously shown that the frequency of defective allele B in the islands of Lake Titicaca is the highest in the world. Now, we want to evaluate the frequency of this allele in Amazonian areas that are more prone to infectious diseases. We have genotyped 94 individuals from Andoas-Loreto and found a higher frequency of the defective allele B compared to other populations (but lower than that from Lake Titicaca). This frequency and the high exposure to tropical diseases make the population of Andoas interesting to study the role of the MBL variants in the development these diseases.
Descritores: Genótipo
Genética Populacional
Grupos Étnicos
Haplótipos
Imunidade Inata/genética
Lectina de Ligação a Manose/genética
Variação Genética
-Epidemiologia Descritiva
Estudos Transversais
Limites: Humanos
Masculino
Adolescente
Adulto
Feminino
Pré-Escolar
Criança
Pessoa de Meia-Idade
Idoso de 80 Anos ou mais
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Id: lil-677709
Autor: Paredes Anaya, Mónica; Lizaraso Soto, Frank; Lissón Abanto, Rosa; Rodríguez, E. Giovanna; Calderón, Jorge R; Rodríguez Zárate, Eduardo; García Nores, Gabriela; Fujita Alarcón, Ricardo.
Título: Variación y distribución genética de los SNP's 19, 43 y 63 del gen de susceptibilidad de diabetes tipo 2 Calpaina 10 (Capn10) en la población peruana / Variation and genetic distribution of SNP's 19, 43 y 63 of the susceptibility gene calpain 10 (Capn10) for the type 2 diabetes in the peruvian population
Fonte: Horiz. méd. (Impresa);5(2):7-11, dic. 2005. tab, graf.
Idioma: es.
Resumo: La diabetes tipo 2 es una enfermedad compleja que tiene un componente genético. Calpaina 10 (CAPN10) es un gen de susceptibilidad para este gen y está localizado en 2q37.3. Pacientes de algunas poblaciones de origen amerindio presentan las frecuencias alélicas de los SNP19, 43 y 63 del gen CAPN10, que sugiere una relación causal entre este gen y la biabetes tipo 2. El origen filogenético común, nos permite suponer que CAPN10 también sería un gen de susceptibilidad en la población peruana nativa y mestiza, lo cual nos motivó a investigar esta relación en nuestra población. Se obtuvieron resultados de la frecuencia alélica de los SNP19, 43 y 63 de CAPN10 en 129 controles normales de Lima, la mayoría de origen mestizo. Además de la prueba del equilibrio de Hardy-Weinberg (H-W) para determinar si la población tiene una distribución genéticamente homogénea en dichos marcadores. Se puede concluir que la prueba de H-W sugiere fuertemente que nuestra población control es adecuada para un estudio de asociación y desequilibrio de ligamiento en pruebas caso-control. Esto a su vez es la base para futuros estudios de asociación y desequilibrio de ligamiento, postulando a CAPN10 como gen de susceptibilidad de diabetes tipo 2 en la población peruana.

Type 2 diabetes is a complex genetic disorder, where the gene calpain 10 (CAPN10) located in 2q37.3, plays an important role. Allele frequencies of SNP19, 43 and 63 are present in affected Amerindian populations and might suggest a possible relationship between CAPN10 and type 2 diabetes. The fact that Amerindian populations has a common phyllogenetic origin was our main motivation for studying this possible relation, because it would suggest' that CAPN10 is a susceptibility gene for native and admixed Peruvian populations. Allelic frequencies of SNP19, 43 and 63 of calpain 10 was obtained of 129 normal controls from Lima, most of them admixed population. Hardy-Weinberg test (H-W) was used in order to determine if the population had a genetically homogenous distribution for the employed molecular markers. It can be concluded that the H-W test strongly suggests that the control population is adequate for an association and linkage disequilibrium in case-control studies. Furthermore, this would mean be thstart of future association and linkage disequilibrium studies where CAPN10 would be considered as a susceptibility gene for type 2 diabetes in Peruvian population.
Descritores: Calpaína/genética
Desequilíbrio de Ligação/genética
/genética
DIABETES MELLITUS TIPO TEMEFOS/genética
Frequência do Gene/genética
Polimorfismo de Nucleotídeo Único/genética
Predisposição Genética para Doença/genética
-Estudos de Casos e Controles
Haplótipos/genética
Limites: Humanos
Masculino
Feminino
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Id: lil-677682
Autor: Sandoval S., José; Madsen, Hans O; Garred, Peter; Fujita, Ricardo.
Título: Alta frecuencia de un haplotipo susceptible del gen Mannose Binding Lectin, en las islas AnapiaSuana del Lago Titicaca / High frequency of a haplotipo capable of the gene Mannose Binding Lectin, in the islands AnapiaSuana of the Lake Titicaca
Fonte: Horiz. méd. (Impresa);2(1/2):60-63, dic. 2002. graf, tab.
Idioma: es.
Resumo: Se determinaron las combinaciones de variantes genéticas en 4 sitios del gen de la proteína asociada al sistema inmune MBL (mannose binding lectin), en 19 pobladores de las islas de Anapia-Suana del Lago Titicaca. Se encuentran solamente 3 combinaciones (haplotipos): LYPB, HYPA y LYPA y se observa alta frecuencia (0.58) del haplotipo defectuoso LYPB en las mencionadas islas sobrepasando a otras frecuencias previamente reportadas en el mundo. Este haplotipo es poco frecuente en poblaciones europeas, asiáticas y africanas. La deficiencia sérica de LYPB predispone a infecciones como tuberculosis, VIH-1 y a enfermedades autoinmunes como artritis reumatoide y lupus eritematoso entre otras. Con estos antecedentes nuestros futuros esfuerzos se dirigirán a investigar la razón de la alta frecuencia de LYPB en estas islas y determinar la de las otras islas aledañas.

We have analized the combination of variants at 4 sites of the gene MBL (mannose binding lectin) which is part of the immune system, in 19 normal individuals from the islands of Anapia-Suana in the Lake Titicaca. OnIy 3 combinations (haplotypes) were registered: LYPB, HYPA and LYPA; the proportion of the pressumed defective haplotype LYPB is 0.58 being the highest ever recorded. This haplotype is rare among European, Asian and African populations. It predisposes to infectious diseases as tuberculosis and AIDS as well as autoimmune diseases like reumatoid arthritis and lupus erithematosumo This finding lead our future efforts to elucidate the reason of this high frequency of LYPB and to determine its proportion in other islands of the Titicaca Lake.
Descritores: Haplótipos/genética
Lectina de Ligação a Manose/genética
Lectinas/genética
Limites: Humanos
Masculino
Adolescente
Feminino
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Bracco, José Eduardo
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Id: lil-458624
Autor: Bracco, José Eduardo; Capurro, Margareth Lara; Lourenço-de-Oliveira, Ricardo; Sallum, Maria Anice Mureb.
Título: Genetic variability of Aedes aegypti in the Americas using a mitochondrial gene: evidence of multiple introductions
Fonte: Mem. Inst. Oswaldo Cruz;102(5):573-580, Aug. 2007. tab, graf, ilus.
Idioma: en.
Projeto: Fapesp; . CNPq.
Resumo: To analyze the genetic relatedness and phylogeographic structure of Aedes aegypti, we collected samples from 36 localities throughout the Americas (Brazil, Peru, Venezuela, Guatemala, US), three from Africa (Guinea, Senegal, Uganda), and three from Asia (Singapore, Cambodia, Tahiti). Amplification and sequencing of a fragment of the mitochondrial NADH dehydrogenase subunit 4 gene identified 20 distinct haplotypes, of which 14 are exclusive to the Americas, four to African/Asian countries, one is common to the Americas and Africa, and one to the Americas and Asia. Nested clade analysis (NCA), pairwise distribution, statistical parsimony, and maximum parsimony analyses were used to infer evolutionary and historic processes, and to estimate phylogenetic relationships among haplotypes. Two clusters were found in all the analyses. Haplotypes clustered in the two clades were separated by eight mutational steps. Phylogeographic structure detected by the NCA was consistent with distant colonization within one clade and fragmentation followed by range expansion via long distance dispersal in the other. Three percent of nucleotide divergence between these two clades is suggestive of a gene pool division that may support the hypothesis of occurrence of two subspecies of Ae. aegypti in the Americas.
Descritores: Variação Genética
Aedes/genética
DNA Mitocondrial/genética
Genética Populacional
Insetos Vetores/genética
NADH Desidrogenase/genética
-Aedes/enzimologia
África
América
Ásia
Haplótipos/genética
Insetos Vetores/enzimologia
Reação em Cadeia da Polimerase
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Bracco, José Eduardo
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Id: lil-417072
Autor: Costa-da-Silva, André Luis da; Capurro, Margareth Lara; Bracco, José Eduardo.
Título: Genetic lineages in the yellow fever mosquito Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Peru
Fonte: Mem. Inst. Oswaldo Cruz;100(6):539-544, Oct. 2005. mapas, tab.
Idioma: en.
Projeto: Sucen; . Fundação de Amparo à Pesquisa do Estado de São Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: The yellow fever mosquito Aedes aegypti was introduced in Peru in 1852 and was considered to be eradicated in 1958. In 2001, Ae. aegypti had been recorded in 15 out of 24 Peruvian Departments. Peru has great ecological differences between the east and west sides of Andes. Because of this, we consider that Ae. aegypti populations of both east and west sides can have a genetically distinct population structure. In this study we examined genetic variability and genealogical relationships among three Ae. aegypti Peruvian populations: Lima, Piura (west Andes), and Iquitos (east Andes) using a fragment of the ND4 gene of the mitochondrial genome. Three haplotypes were detected among 55 samples. Lima and Iquitos showed the same haplotype (Haplotype I), whereas Piura has two haplotypes (Haplotype II and III). Haplotype II is four mutational steps apart from Haplotype I, while Haplotype III is 13 mutational steps apart from Haplotype I in the network. The analysis of molecular variation showed that mostly of the detected genetic variation occurs at interpopulational level. The significant value phist suggests that Piura population is structured in relation to Lima and Iquitos populations and the gene flow of the ND4 is restricted in Piura when compared to Lima and Iquitos. Genetic relationship between haplotype I and haplotype II suggests introduction of the same mtDNA lineage into those localities. However the existence of a genetically distant haplotype III also suggests introduction of at least two Ae. aegypti lineages in Peru.
Descritores: Variação Genética
Aedes/genética
Genes de Insetos/genética
Haplótipos/genética
Insetos Vetores/genética
-Sequência de Bases
Dados de Sequência Molecular
Peru
Polimorfismo de Nucleotídeo Único
Análise de Sequência de DNA
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-408367
Autor: Rodrigues, A. C; Rebecchi, I. M. M; Bertolami, M. C; Faludi, A. A; Hirata, M. H; Hirata, R. D. C.
Título: High baseline serum total and LDL cholesterol levels are associated with MDR1 haplotypes in Brazilian hypercholesterolemic individuals of European descent
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;38(9):1389-1397, Sept. 2005. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: The MDR1 gene encodes the P-glycoprotein, an efflux transporter with broad substrate specificity. P-glycoprotein has raised great interest in pharmacogenetics because it transports a variety of structurally divergent drugs, including lipid-lowering drugs. The synonymous single-nucleotide polymorphism C3435T and the nonsynonymous single-nucleotide polymorphism G2677T/A in MDR1 have been indicated as potential determinants of variability in drug disposition and efficacy. In order to evaluate the effect of G2677T/A and C3435T MDR1 polymorphisms on serum levels of lipids before and after atorvastatin administration, 69 unrelated hypercholesterolemic individuals from São Paulo city, Brazil, were selected and treated with 10 mg atorvastatin orally once daily for four weeks. MDR1 polymorphisms were analyzed by PCR-RFLP. C3435T and G2677T polymorphisms were found to be linked. The allelic frequencies for C3435T polymorphism were 0.536 and 0.464 for the 3435C and 3435T alleles, respectively, while for G2677T/A polymorphism allele frequencies were 0.580 for the 2677G allele, 0.384 for the 2677T allele and 0.036 for the 2677A allele. There was no significant relation between atorvastatin response and MDR1 polymorphisms (repeated measures ANOVA; P > 0.05). However, haplotype analysis revealed an association between T/T carriers and higher basal serum total (TC) and LDL cholesterol levels (TC: 303 ± 56, LDL-C: 216 ± 57 mg/dl, respectively) compared with non-T/T carriers (TC: 278 ± 28, LDL-C: 189 ± 24 mg/dl; repeated measures ANOVA/Tukey test; P < 0.05). These data indicate that MDR1 polymorphism may have an important contribution to the control of basal serum cholesterol levels in Brazilian hypercholesterolemic individuals of European descent.
Descritores: LDL-Colesterol/sangue
Genes MDR/genética
Haplótipos/genética
Hipercolesterolemia/genética
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/genética
-Anticolesterolemiantes/uso terapêutico
Brasil
LDL-Colesterol/genética
Grupo com Ancestrais do Continente Europeu
Frequência do Gene
Ácidos Heptanoicos/uso terapêutico
Hipercolesterolemia/sangue
Hipercolesterolemia/tratamento farmacológico
Hipercolesterolemia/etnologia
Reação em Cadeia da Polimerase
Polimorfismo Genético
Polimorfismo de Fragmento de Restrição
Polimorfismo de Nucleotídeo Único
Pirróis/uso terapêutico
Limites: Adulto
Idoso
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME



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