Base de dados : LILACS
Pesquisa : G05.783 [Categoria DeCS]
Referências encontradas : 139 [refinar]
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Id: lil-778346
Autor: Chagas, Vanessa M; Silva, Madson A. Vidal e; Martins, José H; Santos, Cícero S; Aguiar, Hans M. V. S. B; Barros, Celso H. S. C; Chaves, Ricardo M; Torres-Júnior, José R. S.
Título: Fatores anatomofisiologicos que afetam a qualidade oocitária em bovinos / Anatomophysiologic factors affecting oocyte quality in cattle
Fonte: Pesqui. vet. bras = Braz. j. vet. res;34(supl.1):34-38, dez. 2014. tab.
Idioma: pt.
Projeto: FAPEMA.
Resumo: Para estudar os fatores anatomofisiológicos que interferem na qualidade de complexos cumulus-oócitos (CCOs) bovinos, foram obtidas 396 ovários após abate de 198 fêmeas Bos indicus em frigorífico. Os ovários foram separados por categorias, sendo distribuídos em nulípara vs multípara e com progesterona (P4 - presença de corpo lúteo em um dos ovários) vs sem progesterona (NP4 - ausência de corpo lúteo). Todos os folículos foram mensurados e categorizados em pequenos (<6mm), médios (6 a 9mm) ou grandes (>9mm). Em seguida todos os folículos foram puncionados e os CCOs recuperados e avaliados morfologicamente. Não houve diferença na taxa de recuperação nem na qualidade dos CCOs de fêmeas nulíparas vs multíparas. O percentual de CCOs desnudos/degenerados foi maior no grupo NP4 e os CCOs expandidos foram superiores no grupo P4. A taxa de recuperação e o percentual de CCOs selecionados para PIV (graus I e II) foram similares nos grupos P4 vs NP4. Folículos pequenos apresentam menor taxa de recuperação em comparação aos de tamanho médio e grande, porém o percentual de CCOs de grau I foi superior em folículos pequenos e médios. Diante dos resultados aqui encontrados conclui-se que a categoria da doadora e a progesterona não influenciaram a qualidade de CCOs selecionados para PIV e que folículos menores apresentam de CCOs de melhor qualidade.(AU)

To study the anatomical and physiological factors that affect the bovine cumulus-oocyte complexes (COCs) quality, were examined 396 ovaries obtained after slaughter of 198 Bos indicus females. Ovaries were categorized in nulliparous vs multiparous and progesterone (P4 - a corpus luteum at least one ovary) vs without progesterone (NP4 - absence of corpus luteum). All follicles were measured and categorized into small (<6mm), medium (6 to 9mm) or large (>9mm). Then all follicles were punctured and COCs recovered and assessed morphologically. There was no difference in COCs recovery rate or quality from nulliparous vs multiparous females. The rate of denuded or degenerated oocytes was higher in NP4 and expanded COCs were higher in P4. The COCs recovery and grades I and II rates were similar in P4 vs NP4 groups. Small follicles have lower COCs recovery rate than medium or large follicles, but the grade I oocytes rates was higher in small and medium follicles. In conclusion, the category of donor female and progesterone did not affect the quality of COCs selected for PIV and smaller follicles present best quality of COCs.(AU)
Descritores: Oócitos
Progesterona/análise
Células do Cúmulo
Folículo Ovariano
-Seleção Genética
Limites: Animais
Feminino
Bovinos
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Aguiar, Aurelio Mendes
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Id: lil-450997
Autor: Santos, Mateus Figueiredo; Moro, Gustavo Vitti; Aguiar, Aurélio Mendes; Souza Júnior, Cláudio Lopes de.
Título: Responses to reciprocal recurrent selection and changes in genetic variability in IG-1 and IG-2 maize populations
Fonte: Genet. mol. biol;28(4):781-788, Dec. 2005. tab.
Idioma: en.
Projeto: FAPESP.
Resumo: This paper reports the effects of three cycles of reciprocal recurrent selection (RRS) on the means, genetic variances, and on the genetic correlations for several traits in the IG-1 and IG-2 maize (Zea mays L.) populations. Interpopulation full-sib progenies from cycle zero (C0) and from cycle 3 (C3) of RRS were evaluated in two locations. RRS was highly effective to improve the traits according the objectives of the program: grain yield and prolificacy increased significantly, while plant height, ear height, and ear placement decreased significantly. Genetic variances for all traits decreased significantly from C0 to C3, but the genetic correlations did not change consistently across the cycles of selection. The expected responses to the fourth cycle of RRS and the probability of selecting double-crosses from C3 that outperform those from C0 showed that the decreases in the genetic variances were not great enough to limit the continued improvement of the populations as well as the use of the improved populations as sources of inbred lines to develop commercial hybrids. However, if the magnitudes of the genetic variances continue to decrease, new sources of improved germplasm should be incorporated into both populations to allow the continued improvement of the interpopulation by RRS
Descritores: Variação Genética
Zea mays/genética
-Seleção Genética
Técnicas Genéticas
Responsável: BR26.1 - Biblioteca Central


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Id: biblio-1022023
Autor: Darqui, Flavia S; Radonic, Laura M; López, Nilda; Hopp, H Esteban; López Bilbao, Marisa.
Título: Simplified methodology for large scale isolation of homozygous transgenic lines of lettuce
Fonte: Electron. j. biotechnol;31:1-9, Jan. 2018. ilus, graf, tab.
Idioma: en.
Projeto: (INTA).
Resumo: Background: Lettuce is a globally important leafy vegetable and a model plant for biotechnology due to its adaptability to tissue culture and stable genetic transformation. Lettuce is also crucial for functional genomics research in the Asteraceae which includes species of great agronomical importance. The development of transgenic events implies the production of a large number of shoots that must be differentiated between transgenic and non-transgenic through the activity of the selective agent, being kanamycin the most popular. Results: In this work we adjusted the selection conditions of transgenic seedlings to avoid any escapes, finding that threshold concentration of kanamycin was 75 mg/L. To monitor the selection system, we studied the morphological response of transgenic and non-transgenic seedlings in presence of kanamycin to look for a visual morphological marker. Several traits like shoot length, primary root length, number of leaves, fresh weight, and appearance of the aerial part and development of lateral roots were affected in non-transgenic seedlings after 30 d of culture in selective media. However, only lateral root development showed an early, qualitative and reliable association with nptII presence, as corroborated by PCR detection. Applied in successive transgenic progenies, this method of selection combined with morphological follow-up allowed selecting the homozygous presence of nptII gene in 100% of the analyzed plants from T2 to T5. Conclusions: This protocol allows a simplified scaling-up of the production of multiple homozygous transgenic progeny lines in the early generations avoiding expensive and time-consuming molecular assays.
Descritores: Plantas Geneticamente Modificadas/genética
Alface/genética
-Seleção Genética
Canamicina/análise
Reação em Cadeia da Polimerase
Alface/química
Plântulas
Homozigoto
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1021458
Autor: Li, Demao; Zhang, Ke; Chen, Limei; Ding, Mengxun; Zhao, Minli; Chen, Shulin.
Título: Selection of Schizochytrium limacinum mutants based on butanol tolerance
Fonte: Electron. j. biotechnol;30:58-63, nov. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Key Program for International S&T Cooperation Projects of China.
Resumo: Background: Mutation breeding is one of the most important routes to achieving high docosahexaenoic acid (DHA) productivity using Schizochytrium. However, few selection strategies have been reported that aim to generate a high DHA content in Schizochytrium lipids. Results: First, culture temperature altered the butanol tolerance of Schizochytrium limacinum B4D1. Second, S. limacinum E8 was obtained by selecting mutants with high butanol tolerance. This mutant exhibited a 17.97% lower proportion of DHA than the parent strain S. limacinum B4D1. Third, a negative selection strategy was designed in which S. limacinum F6, a mutant with poor butanol tolerance, was obtained. The proportion of DHA in S. limacinum F6 was 11.22% higher than that of parent strain S. limacinum B4D1. Finally, the performances of S. limacinum B4D1, E8 and F6 were compared. These three strains had different fatty acid profiles, but there was no statistical difference in their biomasses and lipid yields. Conclusion: It was feasible to identified the relative DHA content of S. limacinum mutants based on their butanol tolerance.
Descritores: Ácidos Docosa-Hexaenoicos/biossíntese
Butanóis/metabolismo
Estramenópilas/genética
Estramenópilas/metabolismo
-Seleção Genética
Temperatura Ambiente
Ácido Eicosapentaenoico/metabolismo
Biomassa
Butanóis/toxicidade
Ácidos Graxos/metabolismo
Ácidos Graxos/química
Estramenópilas/efeitos dos fármacos
Fermentação
Mutação
Responsável: CL1.1 - Biblioteca Central


  5 / 139 LILACS  
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Id: biblio-1015856
Autor: He, Meijing; Cui, Shunli; Yang, Xinlei; Mu, Guojun; Chen, Huanying; Liu, Lifeng.
Título: Selection of suitable reference genes for abiotic stress-responsive gene expression studies in peanut by real-time quantitative PCR
Fonte: Electron. j. biotechnol;28:76-86, July. 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . 948 Project; . Special Fund for Modern Agro-industry Technology Research System of China; . Key Technologies Research and Development Program of Hebei Province.
Resumo: Background: Because of its strong specificity and high accuracy, real-time quantitative PCR (RT-qPCR) has been a widely used method to study the expression of genes responsive to stress. It is crucial to have a suitable set of reference genes to normalize target gene expression in peanut under different conditions using RT-qPCR. In this study, 11 candidate reference genes were selected and examined under abiotic stresses (drought, salt, heavy metal, and low temperature) and hormone (SA and ABA) conditions as well as across different organ types. Three statistical algorithms (geNorm, NormFinder and BestKeeper) were used to evaluate the expression stabilities of reference genes, and the comprehensive rankings of gene stability were generated. Results: The results indicated that ELF1B and YLS8 were the most stable reference genes under PEG-simulated drought treatment. For high-salt treatment using NaCl, YLS8 and GAPDH were the most stable genes. Under CdCl2 treatment, UBI1 and YLS8 were suitable as stable reference genes. UBI1, ADH3, and ACTIN11 were sufficient for gene expression normalization in low-temperature experiment. All the 11 candidate reference genes showed relatively high stability under hormone treatments. For organs subset, UBI1, GAPDH, and ELF1B showed the maximum stability. UBI1 and ADH3 were the top two genes that could be used reliably in all the stress conditions assessed. Furthermore, the necessity of the reference genes screened was further confirmed by the expression pattern of AnnAhs. Conclusions: The results perfect the selection of stable reference genes for future gene expression studies in peanut and provide a list of reference genes that may be used in the future.
Descritores: Arachis/genética
Genes de Plantas
Reação em Cadeia da Polimerase em Tempo Real/métodos
-Reguladores de Crescimento de Planta
Padrões de Referência
Seleção Genética
Estresse Fisiológico
Amplificação de Genes
Temperatura Baixa
Perfilação da Expressão Gênica
Secas
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1022620
Autor: Imran, Sana; Maryam, Javed; Nadeem, Asif; Iqbal, Madiha.
Título: Pretentious genomic selection signatures in CYP19A1 gene associated with silent estrous behavior in water buffalo in Pakistan
Fonte: Electron. j. biotechnol;32:35-40, Mar. 2018. ilus, tab.
Idioma: en.
Resumo: Background: Poor reproductive efficiency of river buffalos hampers the production capabilities of animals. Buffalos are mainly considered poor breeders owing to the constrained expression of estrus behavior. Failure to display heat signs is an indication of improper functionality of signaling peptides to trigger on a series of behavioral changes, which can be detectable by breeders for timely insemination of females. This might cause an animal to be a repeat breeder. Genomic variations underlying synthesis of signaling peptides can be a useful marker to select superior animals with better reproductive efficiency. In this context, the current study was designed to analyze the CYP19A1 gene in Nili-Ravi buffalo. Results: A total of 97 animals were selected and were divided into two groups on the basis of their heat score. PCR amplification and sequencing of the amplicons were performed using the specific sets of primer, and then, sequences were analyzed for novel variants. A total of 11 polymorphic sites were identified illustrating phenotypic variation in the heat score. Most of the loci were found homologous. Single Nucleotide Polymorphisms (SNPs) were analyzed for association with silent estrus. A three-dimensional protein model was also generated to locate the position of exonic SNPs. Conclusion: This study illustrated that polymorphic sites in the CYP19A1 gene provided potential markers for selection of buffalos with better estrus behavior.
Descritores: Estro/genética
Búfalos/genética
Aromatase/genética
Sistema Enzimático do Citocromo P-450/genética
-Paquistão
Seleção Genética
Cruzamento
Reação em Cadeia da Polimerase
Polimorfismo de Nucleotídeo Único
Peptídeos e Proteínas de Sinalização Intracelular
Inseminação
Limites: Animais
Feminino
Gravidez
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1009846
Autor: Taski-Ajdukovic, Ksenija; Nagl, Nevena; Curcic, Zivko; Zoric, Miroslav.
Título: Estimation of genetic diversity and relationship in sugar beet pollinators based on SSR markers
Fonte: Electron. j. biotechnol;27:1-7, May. 2017. tab, ilus, graf.
Idioma: en.
Resumo: Background: Genetic diversity studies are important for the selection of parents with a greater combination capacity that, when crossed, increase the chances of obtaining superior genotypes. Thus, 26 polymorphic simple sequence repeat (SSR) primers were used to assess the genetic diversity of 140 individual samples from 12 diploid sugar beet pollinators (pollen parents) and two cytoplasmic male sterile (cms) lines (seed parents). Eight pollinators originated from three research centers in the United States Department of Agriculture, while four pollinators and cms lines were from the Institute of Field and Vegetable Crops, Novi Sad, Serbia. Results: In total, 129 alleles were obtained, with a mean of 3.2 alleles per SSR marker. The observed heterozygosity ranged from 0.00 to 0.87 (mean = 0.30). Expected heterozygosity and Shannon's information index were the lowest for marker BQ590934 and the highest for markers SB15s and FDSB502s; the same markers were the most informative, with PIC values of 0.70 and 0.69, respectively. Three private alleles were found in pollinator EL0204; two in pollinator C51; and one in pollinators NS1, FC221, and C93035. Molecular variance showed that 77.34% of the total genetic variation was attributed to intrapopulation variability. Cluster and correspondence analysis grouped sugar beet pollinators according to the breeding centers, with few exceptions, which indicate that certain amount of germplasm was shared, although centers had their own breeding programs. Conclusions: The results indicate that this approach can improve the selection of pollinators as suitable parental components and could further be applied in sugar beet breeding programs.
Descritores: Pólen/genética
Variação Genética
Beta vulgaris/genética
-Polimorfismo Genético
Sementes/genética
Seleção Genética
Cruzamento
Reação em Cadeia da Polimerase
DNA de Plantas/genética
Repetições de Microssatélites
Polinização
Genótipo
Responsável: CL1.1 - Biblioteca Central


  8 / 139 LILACS  
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Texto completo SciELO Brasil
Lobo, R. B
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Texto completo
Id: biblio-1038648
Autor: Santos, C. L. R; Araújo, C. V; Laureano, M. M. M; Araújo, S. I; Lôbo, R. B.
Título: Interação genótipo x ambiente sobre o desempenho ponderal de animais da raça Nelore na região Centro-Oeste / Genotype environment interaction on the weight gain of animals of the Nelore breed in the Midwest
Fonte: Arq. bras. med. vet. zootec. (Online);71(5):1695-1702, set.-out. 2019. tab.
Idioma: pt.
Resumo: Utilizaram-se registros de pesos corporais padronizados aos 120, 210, 365 e 450 dias de idade, provenientes de 30.481 animais da raça Nelore, progênies de 211 reprodutores acasalados com 19.229 matrizes, oriundos de rebanhos dos estados de Mato Grosso, Mato Grosso do Sul e Goiás, com o objetivo de avaliar a presença de interação genótipo x ambiente entre os estados. As estimativas de herdabilidade entre os estados variaram de 0,09 a 0,14; 0,11 a 0,17; 0,16 a 0,27 e 0,17 a 0,35, respectivamente, para os pesos 120, 210, 365 e 450 dias de idade. As estimativas de correlação genética aditiva entre a mesma característica para os diferentes estados apresentaram valores inferiores a 0,80. As correlações de Spearman entre os valores genéticos para os pesos corporais se reduziram à medida que se aumentou a intensidade de seleção sobre os reprodutores. A presença de interação genótipo x ambiente causa maior impacto sobre a avaliação genética dos reprodutores sob intensidade de seleção elevada, sendo interessante sua consideração no processo de avaliação genética. Estimativas de tendências genéticas para todos os pesos corporais apresentaram-se crescentes ao longo dos anos nos três estados.(AU)

Data of adjusted alive weights at 120, 210, 365 and 450 days of age of 30,481 records of animals of the Nellore beef cattle breed from herds of states of Mato Grosso, Mato Grosso do Sul, and Goiás were used to study the influence of environment genotype interaction on genetic evaluation of sires. Estimates of heritability between the states ranged from 0.09 to 0.14; 0.11 to 0.17; 0.16 to 0.27 and 0.17 to 0.35, respectively for live weights 120, 210, 365 and 450 days of age. The estimates of additive genetic correlation between the same characteristic for the different states presented values lower than 0.80. Spearman correlations between breeding values obtained from live weights of sires lowered as the intensity of selection on sires increased. The presence of environment genotype interaction has greater impact on the genetic evaluation of breeding under high intensity of selection, being an interesting consideration in the process of genetic evaluation. Estimates of genetic trends for all body weights have been increasing over the years in all three states.(AU)
Descritores: Seleção Genética
Bovinos/crescimento & desenvolvimento
Bovinos/genética
Meio Ambiente
Genótipo
Criação de Animais Domésticos/estatística & dados numéricos
Limites: Animais
Bovinos
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Texto completo SciELO Chile
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Id: lil-728312
Autor: Barra, R de la; Latorre, E; Martínez, M. E; Calderón, C.
Título: Morphostructural differentiation and variability of Merino sheep breed under sustained directional selection / Diferenciación y variabilidad morfoestructural de ovinos de raza Merino sometidos a selección direccional sostenida
Fonte: Int. j. morphol;32(3):1069-1073, Sept. 2014. ilus.
Idioma: en.
Resumo: In this paper, the degree of differentiation and morphostructural variability reached by the type of animal resulting from sustained directional selection, in addition to its implications for the emergence of a new sheep breed are analyzed. Twenty-five rams and 200 ewes from a sheep flock submitted to directional selection since 1987 were measured. Thirteen body measurements were taken in other to evaluate the structural morphology and sexual dimorphism. These measurements were compared with identical measures taken in Australian Merino and Marin Magellan Meat Merino sheep breeds. The intrapopulation homogeneity was assessed using the coefficients of variation of the average percentage of body measurements. It is concluded that sustained directional selection has generated the differentiation of the morphostructural format of the subject population compared to the main Merino-type breeds in the Chilean Patagonia. The population shows a similar (and in some cases lower) morphostructural variability than those found in recognized sheep breeds in Chile, so it is possible to state that this population behaves like a different animal group, with breed characteristics.

En el presente trabajo se analiza el grado de diferenciación y variabilidad morfoestructural alcanzado por el tipo de animal resultante de una selección direccional sostenida y sus implicancias en la emergencia de un nuevo grupo racial ovino. Se midieron 25 machos y 200 ovejas adultas. Se tomaron 13 medidas corporales, evaluando la diferenciación de la morfología estructural y el dimorfismo sexual. Estas mediciones se compararon con las realizadas en animales de raza Merino Australiano y Marin Magellan Meat Merino. La homogeneidad intrapoblacional se evaluó a través de los coeficientes de variación porcentual de las medias de las medidas corporales. Se concluye que la selección direccional realizada de forma sostenida ha generado una diferenciación del formato morfoestructural al comparar a la población sujeta a selección con las principales raza de tipo Merino existentes en la Patagonia Chilena. La población evaluada evidencia una variabilidad morfoestructural similar, y en muchos casos menor a la encontrada en razas ovinas reconocidas como tales en Chile, con lo cual es posible señalar que se comporta como un grupo animal distinguible con caracteres de raza.
Descritores: Ovinos/anatomia & histologia
-Seleção Genética
Cruzamento
Chile
Caracteres Sexuais
Limites: Animais
Masculino
Feminino
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
Texto completo SciELO Saúde Pública
Texto completo
Id: biblio-828509
Autor: Medeiros, André Luís dos Santos; Nadanovsky, Paulo.
Título: Car and motorcycle deaths: an evolutionary perspective / Mortes por batida de carro e moto: uma perspectiva evolucionista
Fonte: Ciênc. saúde coletiva;21(12):3691-3702, 2016. tab, graf.
Idioma: en.
Resumo: Abstract Our aim was to assess differences between men and women in the likelihood of exposure to traffic as drivers of cars and motorcycles, and in the risk of dying from a car or a motorcycle crash, in order to verify the extent to which Darwin's Sexual Selection Theory could have predicted the findings and can help to interpret them. Study population was composed of men and women aged 18 to 60 years residents in the state of Rio de Janeiro between 2004 and 2010, and in the state of Rio Grande do Sul between 2001 and 2010. We built frequency distribution tables and drew bar charts in order to check whether there were differences between the sexes and interactions of sex with age. More men exposed themselves to and died in traffic than women, especially the young. Society should have an especially vigilant attitude towards men on the wheel due to their increased innate tendency to exposure to risk. Darwin's sexual selection theory can be an important ally when postulating hypotheses and interpreting epidemiological findings aiming at improving public policies to reduce the excessive number of traffic deaths, especially in societies where machismo is strong or the stimulus to masculinity is exaggerated.

Resumo Objetivamos averiguar diferenças entre homens e mulheres na exposição ao trânsito como motorista de carro e de moto e no risco de morrer por batida desses veículos, para verificar até que ponto a teoria da seleção sexual de Darwin pode predizer os achados e ajudar a interpretá-los. A população de estudo foi composta por homens e mulheres de 18 a 60 anos residentes no Estado do Rio de Janeiro, entre 2004 e 2010, e no Estado do Rio Grande do Sul, entre 2001 e 2010. Elaboramos tabelas de frequência e gráficos de barras para verificar se havia diferenças entre os sexos e interação de sexo com idade. Homens se expuseram e morreram mais no trânsito do que mulheres, especialmente os jovens. A sociedade deveria ter uma atitude especialmente vigilante com homens ao volante devido à tendência inata deles de maior exposição ao risco. A teoria da seleção sexual de Darwin pode ser uma potente aliada na postulação de hipóteses que busquem aperfeiçoar políticas públicas para reduzir a quantidade exagerada de mortes no trânsito, especialmente em sociedades onde o machismo for forte ou o estímulo à masculinidade exagerado.
Descritores: Acidentes de Trânsito/mortalidade
Condução de Veículo/estatística & dados numéricos
Motocicletas/estatística & dados numéricos
Assunção de Riscos
-Acidentes de Trânsito/estatística & dados numéricos
Fatores Etários
Brasil/epidemiologia
Seleção Genética
Fatores Sexuais
Limites: Seres Humanos
Masculino
Feminino
Adolescente
Adulto
Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME



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