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Id: biblio-1363590
Autor: Almeida, Júlia Pereira Carvalho; Vitral, Robert Willer Farinazzo; Lourenço, Aneliese Holetz de Toledo; Coxiponés, Georgia Botafogo Pinheiro das Flores e Cruz D'almas; Polo, Ana Bárbara; Apolônio, Ana Carolina Morais.
Título: Maintenance of sterility in SMS packaging in dental environments: concern with biosafety
Fonte: Acta sci., Health sci;44:e54648, Jan. 14, 2022.
Idioma: en.
Resumo: Healthcare services must be guided by biosafety practices and microbial control. This control is highly influenced by humidity, which directly impacts the maintenance of sterility of the materials used in the appointments. High concentration of moisture, in the form of aerosol, splashes and spills, is caused during dental care. During the COVID-19 times the contamination by aerosol and droplets worries greatly. Considering that it could cause harm to the sterility of an autoclaved material, especially in dental environments, the objective was to evaluate the behavior of SMS sterilization packages (Spunbonded / Meltblown / Spunbonded) against microbial penetration in an aqueous vehicle. SMS of three brands were challenged, equally divided into two groups: virgin and processed (subjected to a single autoclaving cycle). Each specimen was aseptically deposited on Macconkey agar. Subsequently, 5 µL of Escherichia coliATCC 25922 saline solution [108CFU mL-1] was deposited in center of the SMS specimen and the dish incubated at36°C/ 48h. Reading was performed by the presence or absence of bacterial growth typical of the species under the SMS, observed on the back of Petri dish. The lowest penetration rate observed was 60% for one of the brands in the virgin condition, and 75% for two brands in the processed condition. Statistical analysis showed an association between bacterial penetration and the evaluated group, this association being valid only in the virgin condition. The different SMS behave similarly in terms of resistance to bacterial penetration after being processed. The data show that moisture can assist in bacterial transport through sterilized SMS. Therefore, SMS packages are not able to prevent bacterial penetration, and possibly other microorganisms, when in aqueous vehicles, offering a potential risk of breaking the aseptic chain. Thus, care must be taken in routines for handling and storage sterile packaging.
Descritores: Embalagem de Produtos/instrumentação
Contenção de Riscos Biológicos/instrumentação
Consultórios Odontológicos/organização & administração
Umidade/prevenção & controle
-Esterilização/instrumentação
Controle de Infecções/instrumentação
Estudo de Avaliação
Embalagem de Medicamentos/instrumentação
Solução Salina/análise
COVID-19/prevenção & controle
Fenômenos Microbiológicos/imunologia
Tipo de Publ: Estudo de Avaliação
Responsável: BR513.1 - Biblioteca Central


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Id: biblio-974115
Autor: Poletto, Tatiana Vieira; Vieira, Cleide Rosana Werneck; Silva, Carlos Peres; Fracalossi, Debora Machado.
Título: Isolation and Identification of a Potential Amylolytic Probiotic Bacterium from the Gut of Jundiá Catfish, Rhamdia quelen
Fonte: Braz. arch. biol. technol;61:e18161205, 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT This study aimed to isolate a potential probiotic amylolytic strain from the gut of jundiá catfish to improve carbohydrate digestibility in fish. Two of 31 strains isolated from the foregut of Rhamdia quelen were able to grow on starch-agar medium and were considered amylolytic. The strain that presented higher amylolytic potential, based on a qualitative amylase assay, was chosen. The strain was phenotypically characterized and analysed to determine bile and pH tolerance and extracellular quantitative amylase activity. The probiotic candidate, identified as Aeromonas veronii, showed the ability to survive stresses from a range of pH and bile salt conditions and secreted an interesting enzymatic profile, which may exhibit a synergistic effect when combined with the enzymes secreted by the jundiá catfish, improving carbohydrate digestion in the host. The results demonstrated the potential of A. veronii to improve the digestion process in jundiá by providing exogenous enzymes for the breakdown and absorption of nutrients.
Descritores: Aquicultura
Probióticos
Aeromonas veronii
Fenômenos Microbiológicos
-Peixes-Gato
Responsável: BR1.1 - BIREME


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Id: biblio-989950
Autor: Vásquez Hoyos, Pablo; Soto, Fredi; Pinzón, Daniela; González, Diana; Peña, Clara.
Título: Caracterización de pacientes pediatricos con hemocultivos positivos del servicio de cuidado intensivo pediatrico del Hospital San José Bogotá, abril 2012 a 2017 / Description of Pediatric Patients with Positive Blood Cultures from the Pediatric Intensive Care Unit at Hospital de San José in Bogotá, April 2012 to 2017
Fonte: Infectio;23(2):183-188, Apr.-June 2019. graf.
Idioma: es.
Resumo: Resumen Introducción: El cultivo de la sangre es el método más utilizado en la búsqueda de infecciones del paciente pediátrico porque orienta la terapia antimicrobiana. Objetivo: Determinar la incidencia de hemocultivos positivos y su caracterización microbiológica en pacientes de cuidado intensivo pediátrico del Hospital de San José, Bogotá-Colombia. Materiales y métodos: Descripción de hemocultivos positivos en pacientes pediátricos de la unidad desde abril de 2012 a 2017. Se determinó la incidencia de hemocultivos positivos y se describió la población estudiada y los gérmenes aislados incluido su perfl de antibiograma. Resultados: Ingresaron 1773 pacientes a la UCIP, 241 pacientes (13,6%) fueron hemocultivados, de los cuales 80 (33,2%) fueron positivos, pero 50% de estos fueron catalogados como contaminaciones. La mediana de edad fue de 21 meses, con 64% de sexo masculino. El 57% fue ventilado y 45% tuvieron un catéter central. La mortalidad fue de 15,4%. La patología más frecuentemente fue respiratoria (75%). De los gérmenes no contaminantes el más frecuente aislado fue Staphylococcus aureus (30%), seguido de Klebsiella pneumoniae (17,5%) y Streptococcus pneumoniae (17,5%). El germen contaminante más frecuente fue Staphylococcus epidermidis (47,5%). Conclusión: La frecuencia de hemocultivos positivos es baja y es frecuente que se aíslen gérmenes contaminantes. El patrón fue similar a lo reportado por la red GREBO.

Abstract Introduction. Blood culture is the method most used in the search for pediatric infections because it guides the antimicrobial therapy. Objective. To determine the incidence of positive blood cultures and their microbiological characterization in patients of the pediatric intensive care service of San José Hospital, Bogotá-Colombia. Materials and methods: Description of positive blood cultures in pediatric patients of the unit from April 2012 to 2017. The incidence of positive blood cultures was determined and the population studied and the isolated germs were described, including their antibiogram profle. Results: 1773 patients were admitted to the PICU, 241 patients (13.6%) were blood cultures, of which 80 (33.2%) were positive, but 50% of these were classifed as contaminations. The median age was 21 months, with 64% male. 57% were ventilated and 45% had a central catheter. Mortality was 15.4%. The most frequent pathology was respiratory (75%). Of the non-polluting organisms, the most frequent isolate was Staphylococcus aureus (30%), followed by Klebsiella pneumoniae (17.5%) and Streptococcus pneumoniae (17.5%). The most frequent contaminant was Staphylococcus epidermidis (47.5%). Conclusion: The frequency of positive blood cultures is low and polluting organisms are often isolated. The pattern was similar to that reported by the GREBO network.
Descritores: Staphylococcus epidermidis
Cuidados Críticos
Hemocultura
-Staphylococcus aureus
Streptococcus pneumoniae
Infecções Bacterianas
Testes de Sensibilidade Microbiana
Klebsiella pneumoniae
Fenômenos Microbiológicos
Anti-Infecciosos
Limites: Humanos
Masculino
Lactente
Responsável: CO359.1 - ACIN - Asociación Colombiana de Infectologia


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Id: biblio-1048697
Autor: Baran, Alper; Erdogan, Ahmet; Kavaz, Arzu; Adigüzel, Mehmet Cemal.
Título: Some specific microbiological parameters and prevalence of salmonella spp. in retail chicken meat from erzurum province, turkey and characterization of antibiotic resistance of isolates / Alguns parâmetros microbiológicos específicos e prevalência de SalmonellA spp. cortes de carne de frango da província de Erzurum, Turquia e caracterização da resistência aos antibióticos de isolados
Fonte: Biosci. j. (Online);35(3):878-891, may./jun. 2019. tab, ilus.
Idioma: en.
Resumo: Specific microbiological parameters and the presence of Salmonella spp. were investigated in 72 chicken meat samples (36 wings and 36 drumsticks) collected from markets and butcher shops. The specific microbiological parameters were determined using a conventional cultural method and the presence of Salmonella spp. in chicken samples was determined using conventional and immunomagnetic separation (IMS)-polymerase chain reaction (PCR) methods. In addition, antimicrobial susceptibility of the isolates was revealed using the Kirby-Bauer disc diffusion method. The results indicated that 30 of the 72 samples were positive for Salmonella spp. by the conventional method, and 42 of the 72 were positive by the IMS-PCR method. However, 30 of the 72 samples were positive for Salmonella spp. by both methods. The Salmonella spp. isolates were confirmed by the VITEK2 Compact System and PCR. The susceptibilities of the isolates against 10 antibiotics were determined. The results indicated that isolates (27/30) showed the highest susceptibility to gentamycin (90.00%), while the highest resistance was to nalidixic acid and tetracycline at the 100 and 93.34% levels, respectively. These results indicate a high prevalence of Salmonella spp. in poultry meat from Erzurum city, Turkey, and the antimicrobial resistance profile of these isolates should be considered for public health. The results also show that the IMS-PCR technique was superior to the conventional method for detecting Salmonella in poultry meat.KEYWORDS:Chicken meat. Salmonella. IMS. PCR. Antimicrobial. INTRODUCTION Chicken is one of the most popular food products worldwide, because of nutritional, sensorial and economic factors. Chicken is widely consumed in homes and fast-food establishments, but can become contaminated during processing. The contamination of poultry products with Salmonella and other microorganismsis due to unhygienic conditions during the production, processing, distribution, marketing and preparationstages (DOOKERAN et al., 2012). The genus Salmonella includes short rod-shaped, facultative anaerobe, Gram-negative bacteria. Warm-blooded animals and humans are natural hosts for Salmonella spp. Detecting Salmonella spp. during production and before consumption is important to prevent food-borne salmonellosis. A Salmonella infection in humans is usually caused by consuming undercooked meat or other cross-contaminated foods, such as vegetables,milk and eggs (HASSANEIN et al., 2011). According to a report published by the Centres for Disease Control and Prevention (CDC), it is estimated that about 1.2 million people in the US have been exposed to Salmonella infections, and that an average of 23.000 hospitalisations and 450 deaths occur from these infections. The prevalence rates of Salmonella spp. in chicken meat sold in Turkey are 34-68.75%. Not only in Turkey, but in most developing countries, the absence of an epidemiological surveillance system for salmonellosis cases makes it difficult to effectively assess prevalence (KÄFERSTEIN, 2003). However, Received: 09/05/18 Accepted: 05/12/18

Parâmetros microbiológicos específicos e a presença de Salmonella spp. foram investigados em 72 amostras de carne de frango (36 asas e 36 baquetas) coletadas em mercados e açougues. Os parâmetros microbiológicos específicos foram determinados utilizando um método cultural convencional e a presença de Salmonella spp. em amostras de frango foi determinada utilizando métodos de reação em cadeia da polimerase (PCR) por separação convencional e imunomagnética (IMS). Além disso, a suscetibilidade antimicrobiana dos isolados foi revelada pelo método de difusão do disco de Kirby-Bauer. Os resultadosindicaram que 30 das 72 amostras foram positivas para Salmonella spp. pelo método convencional, e 42 das 72 foram positivas pelo método IMS-PCR. No entanto, 30 das 72 amostras foram positivas para Salmonella spp. por ambos os métodos. Os isolados de Salmonella spp. foram confirmados pelo sistema VITEK2 Compact e PCR. As susceptibilidades dos isolados a 10 antibióticos foram determinadas. Os resultados indicaram que os isolados (27/30) apresentaram maior suscetibilidade à gentamicina (90,00%), enquanto a maior resistência foi ao ácido nalidíxico e à tetraciclina nos níveis de 100 e 93,34%, respectivamente. Estes resultados indicam uma alta prevalência de Salmonella spp. em carne de frango da cidade de Erzurum, Turquia, e o perfil de resistência antimicrobiana desses isolados deve ser considerado para a saúde pública. Os resultados também demonstram que a técnica de IMS-PCR foi superior ao método convencional para detecção de Salmonella em carne de frango.
Descritores: Salmonella
Galinhas
Fenômenos Microbiológicos
Anti-Infecciosos
Responsável: BR396.1 - Biblioteca Central


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Id: lil-543997
Autor: Padrão, Manuella da Cruz; Monteiro, Marina Laterça; Maciel, Natália Raye; Viana, Flávia Freitas Cunha Fazoli; Freitas, Nélio Artiles.
Título: Prevalência de infecções hospitalares em unidade de terapia intensiva / Prevalence of nosocomial infection in intensive care unit
Fonte: Rev. Soc. Bras. Clín. Méd;8(2), mar.-abr. 2010. graf.
Idioma: pt.
Resumo: JUSTIFICATIVA E OBJETIVOS: A infecção hospitalar (IH) representa uma condição grave que influencia na morbimortalidade dos pacientes que permanecem internados. Em unidade de terapia intensiva (UTI), os pacientes encontram-se acamados por tempo prolongado, imunodeprimidos, com doenças graves que necessitam de monitoramento invasivo e uso de antibióticos de largo espectro, tornando-os mais suscetíveis às IH. O objetivo deste estudo foi avaliar a prevalência e o perfil microbiológico das IH na UTI, bem como a evolução dos pacientes notificados.MÉTODO: O estudo foi realizado no Hospital Pró-Clínicas no período de fevereiro a dezembro de 2008. Os casos de IH ocorridos foram notificados através da Ficha de Notificação de Infecção Hospitalar e os respectivos dados foram analisados no programa Doctor Clean versão 3.0.3.RESULTADOS: No período do estudo foram notificados 26 casos de IH. Sua prevalência foi no sexo masculino (57,69%), entre a 7ª e 8ª décadas, sendo o trato respiratório o local mais acometido (44,19%) e o micro-organismo mais isolado foi o Proteus mirabilis (22,22%).CONCLUSÃO: A partir dos resultados obtidos pode-se traçar um perfil microbiológico próprio da UTI, sendo possível programar uma terapêutica empírica mais eficaz e direcionada, reduzindo os custos hospitalares, o tempo de internação dos pacientes e o surgimento de micro-organismos multirresistentes.(AU)

BACKGROUND AND OBJECTIVES: The nosocomial infection (NI) is a serious condition that affects the mortality of patients who remain hospitalized. In an Intensive Care Unit (ICU) patients are bedridden for long periods, compromised, with serious illnesses that require invasive monitoring and use of broad spectrum antibiotics, making them more susceptible to NI's. The study aims to assess the prevalence and microbiological profile of NI's in the ICU and the evolution of the patients reported.METHOD: The study was performed at Hospital Pró-Clínicas from February to December of 2008. The cases of IH occurred were reported through the Notification Form Hospital Infection and the data were analyzed with the Doctor Clean program version 3.0.3.RESULTS: Throughout the study period were reported 26 cases of IH. Its prevalence was among males (57.69%), between the 7th and 8th decades, the respiratory tract was the most commonly affected (44.19%) and the most isolated microorganism was Proteus mirabilis (22.22%).CONCLUSION: From the results obtained one can draw a microbiological profile of the ICU itself, it is possible to implement an empirical therapy more accurately and efficiently, reducing hospital costs, length of hospital patients and the emergence of resistant microorganisms.(AU)
Descritores: Proteus mirabilis/isolamento & purificação
Infecções Respiratórias/epidemiologia
Infecção Hospitalar/epidemiologia
Unidades de Terapia Intensiva
-Prevalência
Fenômenos Microbiológicos
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-987978
Autor: Gutiérrez Guzmán, Nelson; Fernández Segovia, Isabel; Fuentes López, Ana; Ruiz Rico, María; Barat Baviera, José Manuel.
Título: Physico-chemical and microbiological changes in commercial tilapia (Oreochromis niloticus) during cold storage / Cambios fisico-químicos y microbiológicos en tilapia comercial (Oreochromis niloticus) durante almacenamiento refrigerado
Fonte: Vitae (Medellín);22(2):140-147, 2015. Ilustraciones.
Idioma: en.
Resumo: Background: The microbiological and chemical processes are the main responsible for deterioration of fresh fish. Therefore, it is essential to avoid these processes by applying good manufacturing practices during fish handling, distribution and storage. Objective: The aim of this paper was to evaluate the physico-chemical and microbial changes in commercial tilapia (Oreochromis niloticus) during cold storage in order to establish the shelf life since its arrival at the supermarket. Methods: 27 aquacultured tilapia specimens were analyzed at 0, 2, 4, and 7 days of storage at 4ºC. Measurements of texture, color, water holding capacity, total volatile basic nitrogen (TVB-N), thiobarbituric acid index, ATP-related compounds, as well as microbial analyses were carried out. Results: The TVB-N content was high at the beginning of the study, remaining stable during the storage. Lipid oxidation of samples was minimum, so this process did not contribute to the fish spoilage. It was observed the breakdown of inosine-5'- monophosphate (IMP) into Ino (inosine), and Ino into Hx (hypoxantine). The texture parameters and colour underwent changes as a consequence of the fish spoilage. Low microbial counts were observed at day 0, but Enterobacteriaceae and mesophilic counts gradually increased throughout storage. Conclusions: The K1 -value showed the progressive spoilage of the fish during the cold storage. The decrease of hardness and firmness confirmed the loss of quality throughout the time of study. The low microbial counts at the beginning of the study demonstrated the good quality of the tilapia; however, the increase of the mesophilic counts at the end of the studied period showed that tilapia was not fit for human consumption at day 7.

Antecedentes: Los procesos microbiológicos y químicos son los principales responsables del deterioro del pescado fresco. Por tanto, es esencial evitar estos procesos aplicando buenas prácticas de fabricación durante la manipulación, distribución y almacenamiento del pescado. Objetivo: El objetivo de este trabajo fue evaluar los cambios físico-químicos y microbiológicos en tilapia comercial (Oreochromis niloticus) durante el almacenamiento refrigerado con el fin de establecer la vida útil desde su llegada al supermercado. Métodos: 27 especímenes de tilapia fueron analizados a día 0, 2, 4 y 7 de almacenamiento a 4 ºC. Se llevaron a cabo medidas de textura, color, capacidad de retención de agua, nitrógeno básico volátil total (N-BVT), índice del ácido tiobarbitúrico, compuestos relacionados con el ATP, así como análisis microbianos. Resultados: El contenido en N-BVT fue alto al principio del estudio, siendo estable durante el almacenamiento. La oxidación lipídica de las muestras fue mínima, por lo que este proceso no contribuyó al deterioro del pescado. Se observó la degradación de inosina-5'- monofosfato (IMP) a Ino (inosina), y de Ino a Hx (hipoxantina). Los parámetros de textura y color sufrieron cambios como consecuencia del deterioro del pescado. Se observaron bajos recuentos microbianos a día 0, pero los recuentos de Enterobacteriaceae y de mesófilos aumentaron gradualmente durante el almacenamiento. Conclusiones: El valor K1 mostró el deterioro progresivo del pescado durante el almacenamiento en refrigeración. La disminución de dureza y firmeza confirmó la pérdida de calidad a lo largo del tiempo de estudio. Los bajos recuentos microbianos al principio del estudio demostraron la buena calidad de la tilapia; sin embargo, el aumento de los recuentos de mesófilos al final del periodo estudiado mostraron que la tilapia no era adecuada para el consumo humado a día 7.
Descritores: Ciclídeos
Fenômenos Químicos
Fenômenos Microbiológicos
-Gestão da Qualidade Total
Limites: Humanos
Tipo de Publ: Artigo Clássico
Responsável: CO56.3 - Biblioteca


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Id: lil-737135
Autor: Salazar Torres, Lay; Ávila Gamboa, Dalton.
Título: Inmunología perinatal / Perinatal immunology
Fonte: Femina;42(4):185-192, jul-ago. 2014.
Idioma: pt.
Resumo: Se ha considerado que el útero gestante es un lugar inmunológicamente privilegiado, donde el feto es protegido del rechazo por el sistema inmune materno, mediante un amplio repertorio de estrategias de evasión que contribuye a la sobrevivencia del feto. La gestación en sí misma constituye un acontecimiento de equilibrio inmunológico y la tolerancia inmunológica permite la progresión del embarazo, donde participan una secuencia sincronizada de eventos que se inicia desde la concepción y fertilización para dar lugar a la implantación y progresa hasta alcanzar un embarazo a término. El sistema inmune es la principal barrera que poseemos para protegernos de las infecciones. Durante la vida intrauterina, el feto está protegido por la madre de las agresiones externas, por lo que no necesita que su sistema inmunológico sea operativo, sin embargo, al nacer, recibe una avalancha de elementos extraños, por lo que necesitará disponer de cierta protección, así como una preparación para ejecutar las defensas necesarias para su protección inmunológica. La inmunidad sérica durante la vida fetal queda limitada a la transferencia a través de la placenta de IgG materna, a pesar de que el feto tiene la facultad de sintetizar inmunoglobulinas desde las primeras etapas de la gestación. Al nacimiento, el niño tiene su sistema inmunológico completo, aunque inmaduro, pero es capaz de responder a los estímulos antigénicos. Tiene múltiples anormalidades en el desarrollo de su sistema inmune, que involucran a los anticuerpos/inmunoglobulinas, complemento y granulocitos pudiendo contribuir a la alta incidencia de sus infecciones. El recién nacido carece de memoria inmunológica debido a que, en condiciones normales, el feto está exento de estímulos producidos por antígenos extraños. Dicha memoria se va adquiriendo a medida que entra en contacto con los diferentes antígenos. Se obtendrá cierta protección a las infecciones entéricas gracias a las IgA que aporta la lactancia materna. La exposición prenatal y postnatal a productos microbianos ambientales que pueden activar la inmunidad innata, puede acelerar el proceso de maduración del sistema inmune.(AU)

It has been considered the pregnant women`s womb as an immunological exceptional place, where fetus is protected against been rejected because of maternal immune system by means of a wide groups of evasive strategies that help in its survival. Pregnancy itself is an immunological equilibrium state and the immunological tolerance allow the progression of this event, where participate a synchronized sequence of biological events started from conception and fertilization to allow the implantation, and progress until to reach the pregnancy end. The immune system is our main barrier against infections. During intrauterine life fetus is protected by the mother against external aggressions, therefore he don`t need an operative immune system, nevertheless, at birth the new organisms receive an avalanche of strange elements needing some kind of protection as well as a preparation to carry out the necessary defense for his immunological protection. Serum immunity during fetal life is limited to the transference of maternal IgG through placenta, despite fetus capability to synthesize immunoglobulins from first stages of gestation. At birth the babe has a complete immunological system although immature but capable to respond to antigenic stimulus. He has multiples abnormalities in the immune system development that take account antibodies/immunoglobulin, complement and granulocytes contributing to his high incidence of infections. Newborn lack immunological memory because in normal conditions fetus is not stimulated by odd antigens. This memory is acquired through the contact with different antigens. It will be obtained some protection against enteric infections because IgA from maternal lactation. The prenatal and postnatal exposition to environmental microbial products that activate the innate immunity can accelerate the immune system maturing process.(AU)
Descritores: Imunoglobulinas/imunologia
Recém-Nascido/imunologia
Recém-Nascido Prematuro/imunologia
Feto/imunologia
Imunidade Materno-Adquirida/imunologia
Anticorpos/imunologia
-Gravidez/imunologia
Linfócitos B/imunologia
Imunidade Adaptativa/imunologia
Fenômenos Microbiológicos/imunologia
Leite Humano/imunologia
Limites: Feminino
Gravidez
Recém-Nascido
Responsável: BR1365.1 - Biblioteca Biomédica A - CB/A


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Id: lil-730325
Autor: La Rosa Hernández, Deyanira; Gómez Cabeza, Enrique José; Sánchez Castañeda, Niurka.
Título: La microbiota intestinal en el desarrollo del sistema inmune del recién nacido / Intestinal microbiota in the development of the neonate's immune system
Fonte: Rev. cuba. pediatr;86(4):502-513, oct.-dic. 2014.
Idioma: es.
Resumo: La microbiota intestinal comprende al conjunto de microorganismos comensales que cohabitan en simbiosis con el individuo. Su programación intraútero y colonización ulterior son factores determinantes en la maduración del sistema inmune. Para enriquecer nuestros conocimientos sobre el efecto de la microbiota intestinal en la maduración del sistema inmune en el niño, se realizó una revisión bibliográfica tras consultar las bases de datos Google, Medline y el Localizador de Información de Salud de Infomed, con la utilización de descriptores como microbiota intestinal, sistema inmune, gut microbiota.

Intestinal microbiota covers the set of commensal microorganisms that live together with the individual. The intrauterine programming and further colonization are determining factors in maturation of the immune system. With the purpose of expanding our knowledge on the effect of the intestinal microbiota in the child´s immune system maturation, a literature review was made supported on Google, Medline databases and the Infomed health information searcher and using intestinal microbiota, immune system, gut microbiota as subject headings.
Descritores: Bactérias/imunologia
Intestinos/microbiologia
Fenômenos Microbiológicos
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-948254
Autor: Marini, María Cecilia; Gasparini, Beatriz; Glineur, Ileana; Vasquez, Gustavo; Marini, Pedro; Fernandez, María; Farinati, Alicia; Leguia, Mercedes.
Título: Biofilm en lentes de contacto hidrofílicas / Biofilm in hydrophilic contact lenses
Fonte: Rev. Hosp. El Cruce;(13):19-23, 20121030.
Idioma: es.
Resumo: INTRODUCCIÓN: Los biofilms son colecciones de microorganismos sésiles rodeadas de una matriz extracelular (exopolisacáridos) que ellos mismos generan. Se hallan de alguna manera, involucrados en la mayoría de los procesos infecciosos. La capacidad de generar biofilm por parte de algunos microorganismos sobre la superficie de las lentes de contacto (LC), dan a esta estructura un lugar relevante en la prevención de queratitis bacterianas en usuarios de lentes de contacto. OBJETIVO: Nuestro objetivo fue evidenciar cualitativamente, de manera reproducible mediante técnicas sencillas, el desarrollo de biofilm bacteriano sobre la superficie de lentes de contacto hidrofílicas usadas. METODOLOGÍA: Se realizó un estudio prospectivo descriptivo, utilizando 17 lentes de contacto hidrofílicas usadas (LCHU) sobre las cuales se evaluó el desarrollo de biofilm de Pseudomonas aeruginosa (ATTC 27853). RESULTADO: Se evidenció la formación de biofilm a las 8 horas de incubación, siendo más evidente a las 10 horas. Los resultados se evaluaron mediante microscopia óptica, de difracción y confocal. CONCLUSIÓN: El reconocimiento del biofilm como responsable de procesos infecciones oculares, entre ellos, las queratitis bacterianas en usuarios de lentes de contacto, posibilita la búsqueda de enfoques novedosos para su tratamiento y prevención.

INTRODUCTION: Biofilms are collections of microorganisms surrounded by a sessile extracellular matrix (exopolysaccharides) generate by themselves. They are involved in most of infectious processes. The ability to generate biofilm by some microorganisms on the surface of contact lenses, gives to bioflim structure an important rol in the prevention of bacterial keratitis in contact lens wearers. OBJECTIVE: Our objective was to show the development of bacterial biofilm on the surface of hydrophilic contact lenses. METHODS: We performed a prospective descriptive study, we evaluated the development of biofilm by Pseudomonas aeruginosa (ATTC 27853) on the surface of 17 hydrophilic contact lenses. After 8 hour of incubation, the formation of biofilm was cleared observed and became more evident 2 hour later. CONCLUSION:The results were evaluated by optical, diffraction and confocal microscopy.
Descritores: Biofilmes
Lentes de Contato Hidrofílicas
Fenômenos Microbiológicos
Tipo de Publ: Artigo Clássico
Responsável: AR24.10 - Hospital El Cruce Néstor Carlos Kirchner


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Id: biblio-916411
Autor: Moraes, Marcelo Soares de; Araújo, Brendon Chaves; Costa, Leonardo Emanuel de Oliveira; Nascimento, Janaína dos Santos.
Título: Avaliação do crescimento de Salmonella enterica em fórmulas lácteas infantis sob diferentes condições de preparo e armazenamento / Evaluation of the growth of Salmonella enterica in infant milk formulas under different preparation and storage conditions
Fonte: Vigil. sanit. debate;3(1):[5], fev. 2015.
Idioma: pt.
Resumo: Salmonella enterica é um dos mais importantes patógenos associados a fórmulas lácteas infantis (FLI). Neste trabalho, foi avaliada a sobrevivência e crescimento de uma estirpe padrão de Salmonella enterica sorotipo Typhi em duas FLI reconstituídas (F1 e F2) sob diferentes condições de preparo e armazenamento similares às utilizadas em lactários hospitalares. Para ambas as FLI inoculadas e incubadas à temperatura ambiente, observou-se um aumento de 3,3 log na população de S. enterica logo nas primeiras 24 horas de incubação e um aumento superior a 4,5 log ao término de 72 horas. A 4ºC, nas primeiras 48 h de incubação, houve leve aumento populacional (< 1,0 log) e, após 72 horas, o crescimento foi similar ao observado sob temperatura ambiente. Em relação ao aquecimento em banho-maria, verificou-se que a 60ºC/ 5 min houve uma redução de um pouco mais de 1 log UFC/ml, entretanto, a 60ºC/ 10 min, a queda observada foi de 2,8 log em F1 e de 2,3 log em F2. Já a 70ºC/ 5 min, ocorreu redução de cerca de 3 log UFC/ml para F1 e F2, enquanto que por 10 min, essa redução foi cerca de 1 log maior. O aquecimento em forno de micro-ondas mostrou ser a forma mais rápida e eficiente de redução da população de Salmonella nas FLI, uma vez que não foi detectada contagem celular após nenhum dos tratamentos utilizados. Os resultados sugerem que FLI contaminadas durante a etapa de preparo podem apresentar um crescimento bacteriano mesmo sob temperatura de refrigeração se mantidos por tempo prolongado e que determinados métodos de tratamento térmico não são suficientes para inibição completa de S. enterica.

Salmonella enterica is one of the most important pathogens associated with infant milk formulas (IMFs). In this study, the survival and growth of an S. enterica serovar Typhi strain in two reconstituted IMFs (F1 and F2) under different conditions of preparation and storage, similar to those used in hospital lactaries, was evaluated. For both IMFs inoculated at room temperature, there was 3.3 log increase in the population of S. enterica by the first 24 h of incubation and > 4.5 log increase at the end of 72 h. At 4ºC, in the first 48 h of incubation, there was a slight increase in population (< 1.0 log) and after 72 h, growth was similar to that observed at room temperature. A heated water bath was used to test the effect of thermal treatment methods on bacterial viability. At 60ºC for 5 min, there was a reduction of slightly more than 1 log CFU/mL; however, at 60ºC for 10 min, the observed decrease was 2.8 log for F1 and 2.3 log for F2. At 70ºC for 5 min, there was a reduction of approximately 3 log CFU/ml for both IMFs and for 10 min, this reduction was approximately 1 log higher. Heating in a microwave oven was the most efficient way of reducing populations of Salmonella in the IMFs, as bacterial cell counts were not detected after any of the treatments used. Our results suggest that IMF contamination during the preparation step may support bacterial growth even under refrigeration if kept for a prolonged time and that some thermal treatment methods are not sufficient for the complete inhibition of S. enterica.
Descritores: Inocuidade dos Alimentos
Alimentos Infantis
Intoxicação Alimentar por Salmonella
-Manipulação de Alimentos
Fenômenos Microbiológicos
Salmonella enterica
Limites: Humanos
Recém-Nascido
Lactente
Responsável: BR2260 - NETHIS - Núcleo de Estudos sobre Bioética e Diplomacia em Saúde



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