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Pesquisa : G06.099 [Categoria DeCS]
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Id: biblio-1087706
Autor: Zhang, Zheng; Yu, Yong-xiang; Wang, Yin-geng; Wei, Xin-xian; Liao, Mei-jie; Rong, Xiao-jun; Chen, Jing.
Título: Development of a new protocol for freeze-drying preservation of Pseudoalteromonas nigrifaciens and its protective effect on other marine bacteria
Fonte: Electron. j. biotechnol;44:1-5, Mar. 2020. graf, tab.
Idioma: en.
Projeto: The Key Projects of Science and Technology Innovation of Shandong Province; . Special Founds of Innovation-driven Development of Guangxi; . Central Public-interest Scientific Institution Basal Research Fund, Yellow Sea Fisheries Research Institutes, CAFS.
Resumo: Background: Freeze-drying is known as one of the best methods to preserve bacterial strains. Protectant is the key factor affecting the survival rate of freeze-dried strains. In addition, salinity, bacterial suspension concentration, drying time, and other factors can also affect the survival rate of strains to varying degrees. At present, there are relatively few studies on freeze-drying preservation of marine bacteria. In the present study, we performed the freeze-drying protectant screening and optimized the preservation conditions for Pseudoalteromonas nigrifaciens, which is widely distributed in marine environment. The protective effects of the screened protectants were verified by 18 other marine bacterial strains. Results: The results indicated that the combination of 5.0% (w/v) lactose, 5.0% (w/v) mannitol, 5.0% (w/v) trehalose, 10.0% (w/v) skim milk powder, 0.5% (w/v) ascorbic acid and 0.5% (w/v) gelatin was the best choice for the preservation of P. nigrifaciens. The suggested salinity and concentration of initial cell suspension were 10 g/L NaCl and 1.0 × 109 CFU/mL, respectively. Furthermore, stationary-phase cells were the best choice for the freeze-drying process. The highest survival rate of P. nigrifaciens reached 52.8% when using 5­10% (w/v) skim milk as rehydration medium. Moreover, the other 18 marine strains belonging to Pseudoalteromonas, Vibrio, Photobacterium, Planomicrobium, Edwardsiella, Enterococcus, Bacillus, and Saccharomyces were freezedried under the abovementioned conditions. Their survival rates were 2.3­95.1%. Conclusion: Collectively, our results supported that the protectant mixture and parameters were beneficial for lyophilization of marine bacteria
Descritores: Preservação Biológica/métodos
Pseudoalteromonas/fisiologia
Liofilização/métodos
-Trealose/química
Sobrevivência Celular
Fenômenos Fisiológicos Bacterianos
Dissacarídeos/química
Viabilidade Microbiana
Salinidade
Lactose/química
Manitol/química
Responsável: CL1.1 - Biblioteca Central


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Id: lil-788975
Autor: Orhan, Furkan.
Título: Alleviation of salt stress by halotolerant and halophilic plant growth-promoting bacteria in wheat (Triticum aestivum)
Fonte: Braz. j. microbiol;47(3):621-627, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT In the current study, 18 halotolerant and halophilic bacteria have been investigated for their plant growth promoting abilities in vitro and in a hydroponic culture. The bacterial strains have been investigated for ammonia, indole-3-acetic acid and 1-aminocyclopropane-1-carboxylate-deaminase production, phosphate solubilisation and nitrogen fixation activities. Of the tested bacteria, eight were inoculated with Triticum aestivum in a hydroponic culture. The investigated bacterial strains were found to have different plant-growth promoting activities in vitro. Under salt stress (200 mM NaCl), the investigated bacterial strains significantly increased the root and shoot length and total fresh weight of the plants. The growth rates of the plants inoculated with bacterial strains ranged from 62.2% to 78.1%.Identifying of novel halophilic and halotolerant bacteria that promote plant growth can be used as alternatives for salt sensitive plants. Extensive research has been conducted on several halophilic and halotolerant bacterial strains to investigate their plant growth promoting activities. However, to the best of my knowledge, this is the first study to inoculate these bacterial strains with wheat.
Descritores: Reguladores de Crescimento de Planta/biossíntese
Estresse Fisiológico
Bactérias/efeitos dos fármacos
Bactérias/metabolismo
Triticum/fisiologia
Triticum/microbiologia
Fenômenos Fisiológicos Bacterianos
Salinidade
-Fenótipo
Raízes de Plantas/fisiologia
Raízes de Plantas/microbiologia
Biomassa
Amônia/metabolismo
Fixação de Nitrogênio
Responsável: BR1.1 - BIREME


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Id: lil-788954
Autor: Akbar, Shamsa; Sultan, Sikander.
Título: Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement
Fonte: Braz. j. microbiol;47(3):563-570, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Background: Since 1960s, the organophosphate pesticide chlorpyrifos has been widely used for the purpose of pest control. However, given its persistence and toxicity towards life forms, the elimination of chlorpyrifos from contaminated sites has become an urgent issue. For this process bioremediation is the method of choice. Results: Two bacterial strains, JCp4 and FCp1, exhibiting chlorpyrifos-degradation potential were isolated from pesticide contaminated agricultural fields. These isolates were able to degrade 84.4% and 78.6% of the initial concentration of chlorpyrifos (100 mg L-1) within a period of only 10 days. Based on 16S rRNA sequence analysis, these strains were identified as Achromobacter xylosoxidans (JCp4) and Ochrobactrum sp. (FCp1). These strains exhibited the ability to degrade chlorpyrifos in sterilized as well as non-sterilized soils, and were able to degrade 93-100% of the input concentration (200 mg kg-1) within 42 days. The rate of degradation in inoculated soils ranged from 4.40 to 4.76 mg-1 kg-1 d-1 with rate constants varying between 0.047 and 0.069 d-1. These strains also displayed substantial plant growth promoting traits such as phosphate solubilization, indole acetic acid production and ammonia production both in absence as well as in the presence of chlorpyrifos. However, presence of chlorpyrifos (100 and 200 mg L-1) was found to have a negative effect on indole acetic acid production and phosphate solubilization with percentage reduction values ranging between 2.65-10.6% and 4.5-17.6%, respectively. Plant growth experiment demonstrated that chlorpyrifos has a negative effect on plant growth and causes a decrease in parameters such as percentage germination, plant height and biomass. Inoculation of soil with chlorpyrifos-degrading strains was found to enhance plant growth significantly in terms of plant length and weight. Moreover, it was noted that these strains degraded chlorpyrifos at an increased rate (5.69 mg-1 kg-1 d-1) in planted soil. Conclusion The results of this study clearly demonstrate that the chlorpyrifos-degrading strains have the potential to develop into promising candidates for raising the productivity of crops in pesticide contaminated soils.
Descritores: Plantas/microbiologia
Bactérias/classificação
Bactérias/metabolismo
Fenômenos Fisiológicos Vegetais
Fenômenos Fisiológicos Bacterianos
Clorpirifos/metabolismo
-Fenótipo
Reguladores de Crescimento de Planta/biossíntese
Microbiologia do Solo
Bactérias/crescimento & desenvolvimento
Biodegradação Ambiental
Responsável: BR1.1 - BIREME


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Id: biblio-839360
Autor: Sinha, Rupesh Kumar; Krishnan, Kottekkatu Padinchati; Hatha, Ammanamveetil Abdulla Mohamed; Rahiman, Mujeeb; Thresyamma, Divya David; Kerkar, Savita.
Título: Diversity of retrievable heterotrophic bacteria in Kongsfjorden, an Arctic fjord
Fonte: Braz. j. microbiol;48(1):51-61, Jan.-Mar. 2017. graf.
Idioma: en.
Resumo: Abstract The diversity and abundance of retrievable pelagic heterotrophic bacteria in Kongsfjorden, an Arctic fjord, was studied during the summer of 2011 (June, August, and September). Retrievable bacterial load ranged from 103 to 107 CFU L−1 in June, while it was 104-106 CFU L−1 in August and September. Based on 16S rRNA gene sequence similarities, a higher number of phylotypes was observed during August (22 phylotypes) compared to that during June (6 phylotypes) and September (12 phylotypes). The groups were classified into four phyla: Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. Bacteroidetes was represented only by a single member Leewenhoekiella aequorea during the three months and was dominant (40%) in June. However, this dominance changed in August to a well-known phytopathogenic species Rhodococcus fascians (32%), which could be a result of decrease in the phytoplankton biomass following the secondary bloom. It is the first report of Halomonas titanicae isolation from the Arctic waters. It showed an increase in its abundance with the intrusion of Atlantic water into Kongsfjorden. Increased abundance of Psychrobacter species in the late summer months coincided with the presence of cooler waters. Thus, the composition and function of heterotrophic bacterial community was fundamentally different in different months. This could be linked to the changes in the water masses and/or phytoplankton bloom dynamics occurring in Arctic summer.
Descritores: Bactérias/isolamento & purificação
Bactérias/classificação
Fenômenos Fisiológicos Bacterianos
Biodiversidade
Processos Heterotróficos
-Regiões Árticas
Bactérias/genética
RNA Ribossômico 16S/genética
Carga Bacteriana
Responsável: BR1.1 - BIREME


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Id: biblio-839357
Autor: Mota, Monalize Salete; Gomes, Cesar Bauer; Souza Júnior, Ismail Teodoro; Moura, Andréa Bittencourt.
Título: Bacterial selection for biological control of plant disease: criterion determination and validation
Fonte: Braz. j. microbiol;48(1):62-70, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul.
Resumo: Abstract This study aimed to evaluate the biocontrol potential of bacteria isolated from different plant species and soils. The production of compounds related to phytopathogen biocontrol and/or promotion of plant growth in bacterial isolates was evaluated by measuring the production of antimicrobial compounds (ammonia and antibiosis) and hydrolytic enzymes (amylases, lipases, proteases, and chitinases) and phosphate solubilization. Of the 1219 bacterial isolates, 92% produced one or more of the eight compounds evaluated, but only 1% of the isolates produced all the compounds. Proteolytic activity was most frequently observed among the bacterial isolates. Among the compounds which often determine the success of biocontrol, 43% produced compounds which inhibit mycelial growth of Monilinia fructicola, but only 11% hydrolyzed chitin. Bacteria from different plant species (rhizosphere or phylloplane) exhibited differences in the ability to produce the compounds evaluated. Most bacterial isolates with biocontrol potential were isolated from rhizospheric soil. The most efficient bacteria (producing at least five compounds related to phytopathogen biocontrol and/or plant growth), 86 in total, were evaluated for their biocontrol potential by observing their ability to kill juvenile Mesocriconema xenoplax. Thus, we clearly observed that bacteria that produced more compounds related to phytopathogen biocontrol and/or plant growth had a higher efficacy for nematode biocontrol, which validated the selection strategy used.
Descritores: Doenças das Plantas/microbiologia
Microbiologia do Solo
Bactérias/classificação
Fenômenos Fisiológicos Bacterianos
-Bactérias/isolamento & purificação
Bactérias/genética
RNA Ribossômico 16S
Enzimas/biossíntese
Rizosfera
Amônia/metabolismo
Hidrólise
Antibiose
Responsável: BR1.1 - BIREME


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Id: biblio-1017057
Autor: Rampadarath, Sillma; Bandhoa, Kushlata; Puchooa, Daneshwar; Jeewon, Rajesh; Bal, Subhasisa.
Título: Early bacterial biofilm colonizers in the coastal waters of Mauritius
Fonte: Electron. j. biotechnol;29:13-21, sept. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Mauritius Research Council for funding the project; . LANDRACE.
Resumo: Background: The past years have witnessed a growing number of researches in biofilm forming communities due to their environmental and maritime industrial implications. To gain a better understanding of the early bacterial biofilm community, microfiber nets were used as artificial substrates and incubated for a period of 24 h in Mauritian coastal waters. Next-generation sequencing technologies were employed as a tool for identification of early bacterial communities. Different genes associated with quorum sensing and cell motility were further investigated. Results: Proteobacteria were identified as the predominant bacterial microorganisms in the biofilm within the 24 h incubation, of which members affiliated to Gammaproteobacteria, Alphaproteobacteria and Betaproteobacteria were among the most abundant classes. The biofilm community patterns were also driven by phyla such as Firmicutes, Bacteroidetes, Chloroflexi, Actinobacteria and Verrucomicrobia. The functional analysis based on KEGG classification indicated high activities in carbohydrate, lipid and amino acids metabolism. Different genes encoding for luxI, lasI, agrC, flhA, cheA and cheB showed the involvement of microbial members in quorum sensing and cell motility. Conclusion: This study provides both an insight on the early bacterial biofilm forming community and the genes involved in quorum sensing and bacterial cell motility.
Descritores: Água do Mar/microbiologia
Bactérias/crescimento & desenvolvimento
Bactérias/genética
Fenômenos Fisiológicos Bacterianos
-Bactérias/isolamento & purificação
Bactérias/classificação
Aderência Bacteriana
Movimento Celular
Biofilmes
Biodiversidade
Percepção de Quorum
Incrustação Biológica
Metagenômica
Sequenciamento de Nucleotídeos em Larga Escala
Maurício
Responsável: CL1.1 - Biblioteca Central


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Id: lil-790063
Autor: Soldatelli Pagno Paim, Roberta; Lorenzini, Elisiane.
Título: Estratégias para prevenção da resistência bacteriana: contribuições para a segurança do paciente / Estrategias para prevención de resistencia bacteriana: contribuciones para la seguridad del paciente / Strategies for prevention of bacterial resistance: contributions to patient safety
Fonte: rev. cuid. (Bucaramanga. 2010);5(2):757-764, july.-dic. 2014. ilus, graf, tab.
Idioma: pt.
Resumo: Introdução: A resistência bacteriana tem como consequências, o aumento da morbidade, mortalidade e da internação hospitalar, o prolongamento das doenças, a perda da produtividade, o aumento dos custos e maior risco de complicações. Materiais e Métodos: Trata-se de um estudo descritivo, com abordagem qualitativa, na perspectiva de uma revisão integrativa da literatura, realizada em janeiro de 2014. Resultados e Discussão: Foram selecionados oito artigos que relatavam estratégias de prevenção da resistência bacteriana em um recorte temporal de 2008 a 2014. O uso racional de antimicrobianos, a higienização adequada das mãos, a cultura de vigilância microbiológica, a educação continuada, a desinfecção de superfícies, uso de testes de suscetibilidade, o isolamento de contato, quando indicado e a manutenção de um banco de dados, são alguns dos métodos encontrados neste trabalho para prevenir a seleção de micro-organismos resistentes. Conclusões: A diminuição do lançamento de novos antimicrobianos pela indústria farmacêutica e a necessidade de novos antibióticos reforça ainda mais a importância da conscientização de toda a equipe multidisciplinar no processo de prevenção de resistência antimicrobiana.

Introducción: La resistencia bacteriana tiene como consecuencias, el aumento de morbilidad, mortalidad y de hospitalización, prolongación de enfermedades, pérdida de productividad, aumento de los costos y mayor riesgo de complicaciones. Materiales y Métodos: Este es un estudio descriptivo con un enfoque cualitativo, en la perspectiva de una revisión integradora de la literatura, realizada en enero del 2014. Resultados y Discusión: Fueron seleccionados ocho artículos que reportan estrategias de prevención de la resistencia bacteriana en un periodo de tiempo del 2008 a 2014. El uso racional de antimicrobianos, la higiene adecuada de las manos, la cultura de vigilancia microbiológica, la educación continuada, la desinfección de superficies, el uso de pruebas de susceptibilidad, aislamiento del contacto, cuando esté indicado y el mantenimiento de una base de datos, son algunos de los métodos encontrados en este trabajo para evitar la selección de microorganismos resistentes. Conclusiones: La disminución en la liberación de nuevos antimicrobianos por la industria farmacéutica y la necesidad de nuevos antibióticos refuerza la importancia de la concientización de todo el equipo multidisciplinario en el proceso de prevención de la resistencia antimicrobiana.

Introduction: Bacterial resistance has as consequences, increased morbidity, mortality and hospitalization, the prolongation of disease, loss of productivity, increased costs and greater risk of complications. Material and Methods: This is a descriptive study with a qualitative approach, from the perspective of a integrative review of literature, held in January 2014. Results and discussion: Were selected eight articles that reported bacterial resistance prevention strategies in a timeframe of 2008 to 2014. The rational use of antimicrobials, proper hand hygiene, microbiological surveillance culture, continuing education, disinfection of surfaces, use of susceptibility tests, contact isolation, when indicated and the maintenance of a database, are some of the methods found in this work to prevent the selection of resistant microorganisms. Conclusions: the decrease in the release of new antimicrobials by the pharmaceutical industry and the need for new antibiotics further strengthens the importance of the awareness of the entire multidisciplinary team in the process of prevention of antimicrobial resistance.
Descritores: Estratégias
Farmacorresistência Bacteriana
Fenômenos Fisiológicos Bacterianos/imunologia
Programa de Controle de Infecção Hospitalar
Resistência à Doença/imunologia
Saúde Pública
Segurança do Paciente
-Antibacterianos
Epidemiologia Descritiva
Literatura de Revisão como Assunto
Serviços de Saúde
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CO646.9 - UDES - Universidad de Santander


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Bolognese, Ana Maria
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Id: biblio-955119
Autor: Santos, André Luis Souza dos; Galdino, Anna Clara Milesi; Mello, Thaís Pereira de; Ramos, Lívia de Souza; Branquinha, Marta Helena; Bolognese, Ana Maria; Columbano Neto, José; Roudbary, Maryam.
Título: What are the advantages of living in a community? A microbial biofilm perspective!
Fonte: Mem. Inst. Oswaldo Cruz;113(9):e180212, 2018. tab, graf.
Idioma: en.
Resumo: Biofilm formation is the preferred mode of growth lifestyle for many microorganisms, including bacterial and fungal human pathogens. Biofilm is a strong and dynamic structure that confers a broad range of advantages to its members, such as adhesion/cohesion capabilities, mechanical properties, nutritional sources, metabolite exchange platform, cellular communication, protection and resistance to drugs (e.g., antimicrobials, antiseptics, and disinfectants), environmental stresses (e.g., dehydration and ultraviolet light), host immune attacks (e.g., antibodies, complement system, antimicrobial peptides, and phagocytes), and shear forces. Microbial biofilms cause problems in the hospital environment, generating high healthcare costs and prolonged patient stay, which can result in further secondary microbial infections and various health complications. Consequently, both public and private investments must be made to ensure better patient management, as well as to find novel therapeutic strategies to circumvent the resistance and resilience profiles arising from biofilm-associated microbial infections. In this work, we present a general overview of microbial biofilm formation and its relevance within the biomedical context.
Descritores: Biofilmes/crescimento & desenvolvimento
Fenômenos Fisiológicos Bacterianos
Fungos/fisiologia
-Microbiologia Ambiental
Responsável: BR1.1 - BIREME


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Id: biblio-894360
Autor: Silva-Junior, Vicente Lopes da; Lopes, Fernanda de Azevedo Marques; Albano, Rodolpho Mattos; Souza, Maria das Graças Coelho de; Barbosa, Carolina Monteiro de Lemos; Maranhão, Priscila Alves; Bouskela, Eliete; Kraemer-Aguiar, Luiz Guilherme.
Título: Obesity and gut microbiota - what do we know so far? / Obesidade e microbiota intestinal - o que sabemos até agora?
Fonte: MedicalExpress (São Paulo, Online);4(4), July-Aug. 2017. tab, graf.
Idioma: en.
Resumo: In the history of medicine, only recently has obesity been recognized as a disease. We know now that it is a pandemic condition, partly explained by the so-called Western lifestyle and related to multiple other comorbidities in various systems. This lyfestyle includes eating large portions, rich in saturated fats and refined sugar, all coupled with sedentary habits. In recent years, the gut microbiota has been indited as a new culprit in pathophysiological aspects involved in obesity. From studies with animals free of bacteria in the digestive tract, known as "germ-free animals", the relevance of intestinal microbiota in the regulation of body fat became evident and its importance has also been extended to the pathophysiology of diseases such as diabetes mellitus and coronary heart disease. Characterization of Toll-like receptors led to the discovery of mechanisms that link the immune system with some metabolic pathways and opened new avenues of a previously unknown world to biological sciences. Increased knowledge about interactions between gut microbiota and the host can certainly reveal, in a not too distant future, new therapeutic perspectives for obesity and its related diseases.

Na história da medicina apenas recentemente a obesidade foi reconhecida como uma doença. Sabemos agora que é uma doença pandêmica, explicada em parte pelo chamado estilo de vida ocidental e relacionado a múltiplas outras comorbidades em vários sistemas. O referido estilo de vida inclui comer grandes porções, ricas em gorduras saturadas e açúcares refinados, e hábitos sedentários. Nos últimos anos, a microbiota intestinal foi associada aos aspectos fisiopatológicos envolvidos na obesidade. De estudos com animais livres de bactérias no trato digestivo, conhecidos como "animais sem germes", a relevância da microbiota intestinal na regulação da gordura corporal tornou-se evidente e sua importância também se estendeu à fisiopatologia de doenças como diabetes mellitus e doença cardíaca coronária. A caracterização dos receptores "Toll-like" levou à descoberta de mecanismos que ligam o sistema imunológico a algumas vias metabólicas e abriram novas avenidas de um mundo anteriormente desconhecido para as ciências biológicas. O aumento do conhecimento sobre as interações entre a microbiota intestinal e o hospedeiro certamente pode revelar, em um futuro não muito distante, novas perspectivas terapêuticas para a obesidade e suas doenças relacionadas.
Descritores: Fenômenos Fisiológicos Bacterianos
Microbioma Gastrointestinal/fisiologia
Intestinos/microbiologia
Obesidade/fisiopatologia
-Doença das Coronárias/etiologia
Diabetes Mellitus/etiologia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Rabinovitch, Leon
Id: biblio-942722
Autor: Rabinovitch, Leon(org); Oliveira, Edmar Justo de(org).
Título: Coletânea de procedimentos técnicos e metodologias empregadas para o estudo de Bacillus e gêneros esporulados aeróbios correlatos.
Fonte: Rio de Janeiro; Fiocruz; 2015. 160 p.
Idioma: pt.
Descritores: Fenômenos Fisiológicos Bacterianos/genética
Bacteriologia/instrumentação
Limites: Masculino
Feminino
Humanos
Responsável: BR1719.1 - Biblioteca do CPqRR
BR1719.1; 610.8, R116c, 2015. 018347



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