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Id: biblio-843175
Autor: Coletti Zabala, Tamara L; Zerbatto, María E; Perotti, Elda B. R; Smacchia, Ana M; Ombrella, Adriana; Pidello, Alejandro R.
Título: Efecto del metronidazol suplementado con hidroquinona en la adherencia de Lactobacillus acidophilus en células vaginales ovinas / Effect of metronidazole supplemented with hydroquinone on the adhesion of Lactobacillus acidophilus in ovine vaginal cells
Fonte: Rev. argent. microbiol;48(4):313-319, dic. 2016. ilus, graf, tab.
Idioma: en.
Resumo: This work demonstrates that the addition of metronidazole together with a ubiquitous quinone compound reduces adherence of Lactobacillus acidophilus to ovine vaginal cells. Spectrophotometric and voltammetric studies have shown that neoformed compounds were observed in these systems; there were also changes in their electroactive composition, and the oxidant status had a significantly higher value compared to the control (p < 0.05). Based on reduction potential (E; mV), the distribution of electroactive compound concentrations suggests that the compounds with low reduction potential induce this behavior, which would indicate that the addition of metronidazole with a ubiquitous quinone compound to the vaginal system might increase the reductive capacity of these systems. This work shows that the study of behavior and fluctuations of the redox compounds that compose the vaginal environment, in terms of concentration and species of redox molecules, must be hierarchized in order to better understand the early stages of colonization by microorganisms.

Este trabajo demuestra que la incorporación de metronidazol conjuntamente con un compuesto quinónico ubicuo disminuye la adherencia de Lactobacillus acidophilus a células vaginales ovinas. Los estudios espectrofotométricos y voltamétricos mostraron que en estos sistemas aparecieron compuestos neoformados y que hubo modificaciones en la composición electroactiva; asimismo, el estatus oxidante presentó un valor significativamente superior con respecto al control (p < 0,05). Según los potenciales de reducción (E; mV), la distribución de las concentraciones de los compuestos electroactivos muestra que los compuestos con bajos potenciales de reducción inducen este comportamiento. Esto indicaría que la incorporación de esta mezcla al sistema vaginal aumentaría su capacidad reductora. El trabajo muestra que el estudio del comportamiento y las fluctuaciones de los compuestos redox que componen el ambiente vaginal, en términos de concentración y especies moleculares, debe ser jerarquizado para comprender mejor las primeras etapas de la colonización de este ambiente por parte de los microorganismos.
Descritores: Doenças dos Ovinos/prevenção & controle
Doenças Vaginais/veterinária
Aderência Bacteriana/efeitos dos fármacos
Metronidazol/análise
-Oxirredução/efeitos dos fármacos
Espectrofotometria/métodos
Ovinos/microbiologia
Metronidazol/farmacologia
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Estudo Observacional Veterinário
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1002295
Autor: Asmat-Abanto, Angel S; Ávila-Rodríguez, Alexandra F; Mejía-Delgado, Elva.
Título: Adhesión Bacteriana en Hilo de Sutura de Seda Negra y Nylon Post Extracción Dental Simple: Ensayo Clínico Controlado / Bacterial Adhesion in Black Silk Suture Thread and Nylon Post Simple Dental Extraction: Controlled Clinical Trial
Fonte: Int. j. odontostomatol. (Print);13(2):132-136, jun. 2019. tab.
Idioma: es.
Resumo: RESUMEN: El objetivo de este estudio fue comparar los hilos de sutura de seda negra y la sutura nylon con respecto a la adhesión bacteriana post extracción dental simple de piezas posteriores. El presente trabajo se desarrolló en la Clínica Odontológica y el Laboratorio de Microbiología de la Unidad de Ciencias Biomédicas y Biotecnología de la Universidad Privada Antenor Orrego (Trujillo, Perú), entre agosto y setiembre de 2014. Se trabajó con 19 unidades de muestreo para hilo de seda negra y 19 para nylon. El procedimiento de exodoncia para todos los pacientes fue estandarizado. Luego de la extracción, se procedió a suturar la herida quirúrgica, colocando dos puntos circunferenciales, uno con cada tipo de hilo. A los 7 días, los hilos fueron retirados y sometidos a análisis microbiológico. La adhesión bacteriana de los hilos de sutura seda negra y nylon fue comparada empleando el test de Wilcoxon. Además la adhesión bacteriana de cada hilo fue comparada con la adhesión en hilos estériles (control) empleando el test de Mann-Whitney para una p<0,005. Al comparar la adhesión bacteriana de ambos hilos, se encontró diferencia entre ellos (p=0,027), correspondiendo mayor adhesión al hilo de nylon. La adhesión bacteriana fue mayor en el hilo de sutura de nylon comparado con el hilo de sutura de seda negra.

ABSTRACT: The objective of the study was to compare black silk suture threads and nylon suture with respect to bacterial adhesion after simple dental extraction of posterior pieces. The present study was developed in the Clínica Estomatológica and the Laboratorio de Microbiología of the Unidad de Ciencias Biomédicas y Biotecnología de la Universidad Privada Antenor Orrego (Trujillo, Peru), between August and September 2014. We worked with 19 units sampling for black silk thread and 19 for nylon. The procedure of exodontia for all patients was standardized. After the extraction, the surgical wound was sutured, placing two circumferential points, one with each type of thread. After 7 days, the threads were removed and subjected to microbiological analysis. The bacterial adhesion of the black silk and nylon suture strands was compared using the Wilcoxon test. In addition, the bacterial adhesion of each strand was compared with the adhesion in sterile strands (control) using the Mann-Whitney test for p <0.005. When comparing the bacterial adhesion of both threads, a difference was found between them (p = 0.027), corresponding greater adhesion to the nylon thread. Bacterial adhesion was higher in nylon suture compared to black silk suture.
Descritores: Extração Dentária/efeitos adversos
Aderência Bacteriana/efeitos dos fármacos
Técnicas de Sutura/instrumentação
-Peru
Suturas/efeitos adversos
Seda
Nylons
Limites: Humanos
Adolescente
Adulto
Pessoa de Meia-Idade
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-891079
Autor: Tupinambá, Rogerio Amaral; Claro, Cristiane Aparecida de Assis; Pereira, Cristiane Aparecida; Nobrega, Celestino José Prudente; Claro, Ana Paula Rosifini Alves.
Título: Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
Fonte: Dental press j. orthod. (Impr.);22(4):77-85, July-Aug. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Introduction: Plasma-polymerized film deposition was created to modify metallic orthodontic brackets surface properties in order to inhibit bacterial adhesion. Methods: Hexamethyldisiloxane (HMDSO) polymer films were deposited on conventional (n = 10) and self-ligating (n = 10) stainless steel orthodontic brackets using the Plasma-Enhanced Chemical Vapor Deposition (PECVD) radio frequency technique. The samples were divided into two groups according to the kind of bracket and two subgroups after surface treatment. Scanning Electron Microscopy (SEM) analysis was performed to assess the presence of bacterial adhesion over samples surfaces (slot and wings region) and film layer integrity. Surface roughness was assessed by Confocal Interferometry (CI) and surface wettability, by goniometry. For bacterial adhesion analysis, samples were exposed for 72 hours to a Streptococcus mutans solution for biofilm formation. The values obtained for surface roughness were analyzed using the Mann-Whitney test while biofilm adhesion were assessed by Kruskal-Wallis and SNK test. Results: Significant statistical differences (p< 0.05) for surface roughness and bacterial adhesion reduction were observed on conventional brackets after surface treatment and between conventional and self-ligating brackets; no significant statistical differences were observed between self-ligating groups (p> 0.05). Conclusion: Plasma-polymerized film deposition was only effective on reducing surface roughness and bacterial adhesion in conventional brackets. It was also noted that conventional brackets showed lower biofilm adhesion than self-ligating brackets despite the absence of film.

RESUMO Introdução: a deposição de filme de polímero a plasma foi criada para modificar as propriedades de superfície dos braquetes ortodônticos metálicos, com o intuito de inibir a adesão bacteriana. Métodos: filmes finos de polímero de hexametildisiloxano (HMDSO) foram depositados em braquetes ortodônticos de aço inoxidável convencionais (n = 10) e autoligáveis (n = 10), utilizando a técnica de radiofrequência PECVD (Plasma-Enhanced Chemical Vapor Deposition). As amostras foram divididas em dois grupos, de acordo com o tipo de braquete, e dois subgrupos após o tratamento de superfície. A microscopia eletrônica de varredura (MEV) foi realizada para avaliar a presença de adesão bacteriana sobre as superfícies das amostras (região de ranhura horizontal e aletas) e a integridade da camada de filme. A Interferometria Confocal (CI) avaliou a rugosidade, e a molhabilidade superficial foi avaliada por goniometria. Para análise de adesão bacteriana, as amostras foram expostas durante 72 horas a uma solução de Streptococcus mutans, para formação de biofilme. Os valores obtidos para a rugosidade da superfície foram analisados pelo teste de Mann-Whitney, enquanto a adesão do biofilme foi avaliada pelos testes de Kruskal-Wallis e SNK. Resultados: observaram-se diferenças estatisticamente significativas (p <0,05) para a rugosidade superficial e redução da adesão bacteriana em braquetes convencionais após o tratamento da superfície, e entre braquetes convencionais e autoligáveis. Não foram observadas diferenças estatísticas significativas entre os grupos autoligáveis (p> 0,05). Conclusão: a deposição de polímero a plasma só foi efetiva na redução da rugosidade superficial e adesão bacteriana em braquetes convencionais. Observou-se, também, que os braquetes convencionais apresentaram menor adesão ao biofilme do que os braquetes autoligáveis, apesar da ausência de filme.
Descritores: Siloxanas/farmacologia
Streptococcus mutans/efeitos dos fármacos
Streptococcus mutans/fisiologia
Aderência Bacteriana/efeitos dos fármacos
Braquetes Ortodônticos/microbiologia
Biofilmes/efeitos dos fármacos
-Plasma
Streptococcus mutans/isolamento & purificação
Propriedades de Superfície
Desenho de Aparelho Ortodôntico
Polimerização
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
Texto completo
Id: biblio-899743
Autor: Villalpando-Guzmán, Sergio; Vázquez-Quiñones, Carlos Ramón; Natividad-Bonifacio, Iván; Curiel-Quesada, Everardo; Quiñones-Ramírez, Elsa Irma; Vázquez-Salinas, Carlos.
Título: Frecuencia, susceptibilidad antimicrobiana y patrón de adherencia de Salmonella enterica aislada de carne de pollo, res y cerdo de la Ciudad de México / Frequency, antimicrobial susceptibility and adherence patterns of Salmonella enterica isolated from chicken meat, beef and pork from Mexico City
Fonte: Rev. chil. infectol;34(5):458-466, oct. 2017. tab, graf.
Idioma: es.
Resumo: Resumen Introducción: Los alimentos de origen animal frecuentemente están implicados en brotes de salmonelosis. Objetivo: Evaluar la frecuencia de Salmonella enterica en carnes molidas de pollo, res y cerdo (un total de 2.592 muestras) obtenidas de mercados sobre ruedas y supermercados de la Delegación Iztapalapa en la Ciudad de México, determinar la susceptibilidad antimicrobiana y efectuar ensayos de adherencia en las cepas aisladas. Métodos: El aislamiento de S. enterica se hizo de acuerdo a la BAM-FDA, la susceptibilidad antimicrobiana de acuerdo con CLSI y el ensayo de adherencia en células HEp-2 conforme a Baffone y cols., 2001. Resultados: Salmonella enterica fue aislada en 511 del total de muestras analizadas (19,7%), de las cuales 244 (47,7%), 152 (29,7%) y 115 (22,5%) correspondieron a carne molida de pollo, res y cerdo, respectivamente. La mayor frecuencia de resistencia de S. enterica a antimicrobianos fue a ampicilina y cloranfenicol en pollo, perfloxacina y ampicilina en res y carbenicilina, ampicilina, cloranfenicol, cefotaxima y perfloxacina en cerdo. Noventa por ciento de las cepas mostraron un patrón de adherencia agregativo. Conclusión: La frecuencia de S. enterica en productos cárnicos es alta, por lo que es importante la adecuada cocción de la carne para disminuir el riesgo de una salmonelosis.

Background: Food of animal origin is often involved in salmonellosis outbreaks. Aim: To evaluate the frequency of Salmonella enterica in chicken, beef and pork ground meat (a total of 2,592 samples) obtained from travelling markets and supermarkets at the Iztapalapa area of Mexico City, in order to determine the antimicrobial susceptibility and adherence capacity of isolated strains. Methods: Isolation of S. enterica was carried out according to the BAM-FDA, the microbial susceptibility according with CLSI and adherence assay on HEp-2 cell line according with Baffone et al., 2001. Results: S. enterica was isolated from 511 of all the analyzed samples (19.7%), from which 244 (47.7%), 152 (29.7%) and 115 (22.5%) corresponded to chicken, beef and pork ground meat, respectively. The highest frequency of resistance of S. enterica to antimicrobials was to ampicillin and chloramphenicol in chicken, perfloxacin and ampicillin in beef and carbenicillin, ampicillin, chloramphenicol, cefotaxime and perfloxacin in pork. Ninety percent of the strains showed an aggregative adherence pattern on HEp-2 cells. Conclusion: The frequency of S. enterica on meat products is high, which is the reason why a proper cooking of these ground meats is important in order to reduce the risk of acquiring salmonellosis.
Descritores: Aves Domésticas/microbiologia
Aderência Bacteriana/fisiologia
Salmonella enterica/isolamento & purificação
Salmonella enterica/efeitos dos fármacos
Carne Vermelha/microbiologia
Antibacterianos/farmacologia
-Suínos
Fatores de Tempo
Resistência Microbiana a Medicamentos
Bovinos
Testes de Sensibilidade Microbiana
Galinhas
Linhagem Celular Tumoral/microbiologia
Sorogrupo
Microbiologia de Alimentos
México
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1282062
Autor: Makkeyah, Fatma Mostafa; Morsi, Tarek Salah; Wahsh, Marwa Mohamed; El-Etreby, Amr Saleh.
Título: An in vitro evaluation of surface roughness, color stability and bacterial accumulation of lithium disilicate ceramic after prophylactic periodontal treatment / Avaliação in vitro da rugosidade superficial, estabilidade de cor e acúmulo bacteriano em cerâmica de dissilicato de lítio após tratamento de profilaxia periodontal
Fonte: Braz. dent. sci;24(3):1-8, 2021. ilus.
Idioma: en.
Resumo: Objective: To evaluate the effect of scaling procedures using different ultrasonic tips on the surface roughness, color stability and bacterial accumulation of lithium disilicate ceramic. Material and Methods: Scaling procedure was carried out using ultrasonic scaler (Satalec, Acteon, North America) with stainless-steel tip (US), titanium tip (UT) and plastic tip (UP), on disc shaped lithium disilicate samples cemented into a cavity prepared onto the labial surface of freshly extracted bovine teeth (10 samples per group). The samples were stored in coffee solution in an incubator at 37°C for 12 days, which is equivalent to 1 year of coffee consumption. The surface roughness was measured before and after the scaling procedure using a profilometer and atomic force microscopy. The color parameters were measured before and after scaling and staining procedures using VITA Easyshade Advance 4.0 according to the CIE L*a*b* color order system. The samples were then incubated with Streptococcus mutans (S. mutans) suspension. After incubation, the plates with 30 to 300 typical colonies of S. mutans were counted in a colony counter and mean values of colony forming units were obtained (CFU/mL). Results:The titanium scaling tip showed a statistically significant higher mean values of change in surface roughness ΔRa and bacterial count than the plastic scaling tip. Color changes (ΔE) were not a statistically significant among the groups. The results showed a statistically significant positive (direct) correlation between surface roughness and color change (p = 0.012) and also between surface roughness and bacterial count (p = 0.00). Conclusion: Within the limitations of this study, titanium scaling instruments cause irreversible surface alterations of lithium disilcate ceramics which was in direct correlation to the color changes and bacterial accumulation; therefore, dentists should proceed with caution when scaling lithium disilicate surfaces. The findings of the current study may indicate the need for instruments or equipment that can remove plaque and calculus without causing surface damage (AU)

Introdução: Avaliar o efeito de procedimentos de raspagem com diferentes pontas de ultrassom na rugosidade superficial, estabilidade de cor e acúmulo bacteriano em cerâmica de dissilicato de lítio. Material e Métodos: O procedimento de raspagem foi realizado usando um aparelho de ultrassom (Satalec, Acteon, América do Norte) com ponta de aço inoxidável (US), ponta de titânio (UT) e ponta de plástico (UP), em amostras de dissilicato de lítio em forma de disco cimentadas em uma cavidade preparada na superfície vestibular de dentes bovinos recém-extraídos (10 amostras por grupo). As amostras foram armazenadas em solução de café em incubadora a 37 ° C por 12 dias, o que equivale a 1 ano de consumo de café. A rugosidade da superfície foi medida antes e após o procedimento de raspagem usando um perfilômetro e um microscópio de força atômica. Os parâmetros de cor foram medidos antes e depois dos procedimentos de raspagem e armazenagem no café usando VITA Easyshade Advance 4.0 de acordo com o sistema de ordem de cores CIE L*a*b*. As amostras foram incubadas com suspensão de Streptococcus mutans (S. mutans). Após a incubação, as placas com 30 a 300 colônias típicas de S. mutans foram contadas em contador de colônias e obtidos os valores médios das unidades formadoras de colônias (UFC / mL). Resultados: A ponta de titânio mostrou valores estatisticamente maiores de mudança na rugosidade da superfície ΔRa e contagem de bactérias do que a ponta de raspagem de plástico. A mudança de cor (ΔE) não foi estatisticamente significativa entre os grupos. Os resultados mostraram uma correlação positiva (direta) estatisticamente significativa entre rugosidade superficial e alteração de cor (p = 0,012) e também entre rugosidade superficial e contagem bacteriana (p = 0,00). Conclusão: Dentro das limitações deste estudo, os instrumentos de raspagem de titânio causam alterações irreversíveis na superfície das cerâmicas de dissilicato de lítio que estão em correlação direta com as mudanças de cor e o acúmulo de bactérias. Portanto, os dentistas devem proceder com cautela ao realizar raspagem em superfícies de dissilicato de lítio. Os resultados deste estudo podem indicar a necessidade de instrumentos ou equipamentos que possam remover a placa e cálculo sem causar danos à superfície. (AU)
Descritores: Propriedades de Superfície
Ultrassom
Aderência Bacteriana
Raspagem Dentária
Cor
Tipo de Publ: Estudo Comparativo
Responsável: BR243.1 - Serviço Técnico de Biblioteca e Documentação


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Timenetsky, Jorge
Texto completo
Id: biblio-950734
Autor: Amorim, Aline Teixeira; Marques, Lucas Miranda; Santos, Angelita Maria Oliveira Gusmão; Martins, Hellen Braga; Barbosa, Maysa Santos; Rezende, Izadora Souza; Andrade, Ewerton Ferraz; Campos, Guilherme Barreto; Lobão, Tássia Neves; Cortez, Beatriz Araujo; Monezi, Telma Alvez; Machado-Santelli, Glaucia Maria; Timenetsky, Jorge.
Título: Apoptosis in HEp-2 cells infected with Ureaplasma diversum
Fonte: Biol. Res;47:1-9, 2014. ilus, graf.
Idioma: en.
Projeto: CNPq; . FAPESB; . FAPESP.
Resumo: BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells.
Descritores: Ureaplasma/patogenicidade
Infecções por Ureaplasma/fisiopatologia
Apoptose/fisiologia
-Fatores de Tempo
Ureaplasma/efeitos dos fármacos
Aderência Bacteriana
Citoesqueleto de Actina/ultraestrutura
Gentamicinas/farmacologia
Células HeLa/microbiologia
Expressão Gênica
Sobrevivência Celular
Fator de Necrose Tumoral alfa/metabolismo
Estatísticas não Paramétricas
Microscopia Confocal
Caspase 3/metabolismo
Caspase 2/metabolismo
Caspase 9/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Padrões Moleculares Associados a Patógenos/metabolismo
Limites: Humanos
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Texto completo
Id: biblio-957466
Autor: Sohail, Muhammad; Latif, Zakia.
Título: Molecular analysis, biofilm formation, and susceptibility of methicillin-resistant staphylococcus aureus strains causing community- and health care-associated infections in central venous catheters
Fonte: Rev. Soc. Bras. Med. Trop;51(5):603-609, Sept.-Oct. 2018. tab, graf.
Idioma: en.
Projeto: Higher Education Commission.
Resumo: Abstract INTRODUCTION: The behavior of methicillin-resistant Staphylococcus aureus (MRSA) isolated from central venous catheter-related infection was evaluated to determine its biofilm potential, antimicrobial resistance, and adhesion genes. METHODS: A total of 1,156 central venous catheters (CVC) were evaluated to screen for pathogens. Antimicrobial sensitivity, biofilm formation potential, and molecular analysis of MRSA were examined following standard guidelines. RESULTS: Of the 1,156 samples, 882 (76%) were colonized by bacteria or candida. Among the infected patients, 69% were male and 36% were female with median age of 32 years. Staphylococcus aureus infected 39% (344/882) of CVCs in patients. Of the 59% (208/344) of patients with MRSA, 57% had community acquired MRSA and 43% had hospital acquired MRSA. Linezolid and vancomycin killed 100% of MRSA; resistance levels to fusidic acid, doxycycline, clindamycin, azithromycin, amikacin, trimethoprim-sulfamethoxazole, gentamycin, tobramycin, and ofloxacin were 21%, 42%, 66%, 68%, 72%, 85%, 95%, 97%, and 98% respectively. Strong biofilm was produced by 23% of samples, moderate by 27%, and weak by 50% of MRSA. The presence of adhesion genes, sdrC and sdrD (90%), eno (87%), fnbA (80%), clfA and sdrE (67%), fnbB, sdrD (61%), and cna (51%), in most MRSA samples suggested that the adhesion genes are associated with biofilm synthesis. CONCLUSIONS: The superbug MRSA is a major cause of CVC-related infection. Antibiotic resistance to major classes of antibiotics and biofilm formation potential enhanced superbug MRSA virulence, leading to complicated infection. MRSA causes infection in hospitals, communities, and livestock.
Descritores: Infecções Estafilocócicas/microbiologia
Infecção Hospitalar/microbiologia
Infecções Comunitárias Adquiridas/microbiologia
Biofilmes/crescimento & desenvolvimento
Staphylococcus aureus Resistente à Meticilina/fisiologia
Infecções Relacionadas a Cateter/microbiologia
Antibacterianos/farmacologia
-Aderência Bacteriana/genética
Testes de Sensibilidade Microbiana
Biofilmes/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Genes Bacterianos/genética
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1247414
Autor: Calderón, Doris Eliana; Pérez, Nancy Estefanía; Quintuña, Jéssica Priscilla; Sanango, Rosa Carolina; Tello, Mónica Priscilla.
Título: Bacterial colonization of composite resins used with direct technique: a brief review
Fonte: Int. j. med. surg. sci. (Print);6(3):101-104, sept. 2019.
Idioma: en.
Resumo: In restorative dentistry, the use of composite resins with direct technique for the replacement of missing tooth structure is very common. One drawback is that surface roughness allows the adherence of microorganisms and the formation of dental plaque, being the polishing technique a key stage in the restoration process. The aim of this paper is to review the process of bacterial colonization of composite resins used with direct technique. According to in vitro studies, bac-terial adhesion on microhybrid composite resins is 3.91 ± 0.52 UFC and on nanohybrid is 3.34 ± 0.74 UFC. Resins with particle size of 2.5 micrometers contained a greater volume of biofilms and enabled adhesion of S. mutans; in turn, resins with particle size of 0.1 to 0.4 micrometers showed lower bacterial adherence. As summary, the degree of bacterial colonization depends on hygiene, polishing technique and composition of restorative material: the bigger the particle size, the greater the adhesion of bacterial plaque.
Descritores: Aderência Bacteriana
Resinas Compostas/química
Tipo de Publ: Revisão
Responsável: CL61.1 - Biblioteca Central Campus Sur


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Id: biblio-1132187
Autor: Gonçalves, Isabella Salgado; Viale, Ana Beatriz; Sormani, Natália Navarro; Pizzol, Karina Eiras Dela Coleta; Araujo-Nobre, Alyne Rodrigues de; Oliveira, Paula Chagas Silva de; Barud, Hélida Gomes de Oliveira; Antonio, Selma Gutierrez; Barud, Hernane da Silva.
Título: Antimicrobial Orthodontic Wires Coated with Silver Nanoparticles
Fonte: Braz. arch. biol. technol;63:e20190339, 2020. graf.
Idioma: en.
Projeto: FAPESP/CEPID; . CNPq.
Resumo: Abstract Conventional orthodontic treatment with the use of stainless steel may be detrimental to oral health by promoting demineralizing lesions appearance and increasing adhesion and formation of bacterial biofilm, inducing the development of cavities. An alternative that has been researched to reduce the side effects of orthodontic treatment is the coating of materials with antimicrobial nanoparticles. Nanometric- sized particles increase their surface area and contact with the microbial membrane, consequently intensifying their bacteriostatic and bactericidal effect. In this work, hydrothermal synthesis, a "green" process was used to attach silver nanoparticles (AgNPs) to the surface of two different brands of orthodontic wires. The coated materials were analyzed for their physicochemical properties by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetry (DSC), which showed the distribution of AgNPs along the wires without modifying their properties. In the microbiological test, one of the brands showed a statistically significant difference in microbial adhesion and biofilm formation by Staphylococcus aureus and Streptococcus mutans. Results lead to the conclusion that antimicrobial orthodontic wires coated with silver nanoparticles through hydrothermal synthesis is a promising material for the improvement of orthodontic treatment.
Descritores: Fios Ortodônticos
Staphylococcus aureus/efeitos dos fármacos
Streptococcus mutans/efeitos dos fármacos
Nanopartículas Metálicas
Antibacterianos/farmacologia
-Prata
Aderência Bacteriana
Calorimetria
Microscopia Eletrônica de Varredura
Placa Dentária
Revestimentos
Responsável: BR1.1 - BIREME


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Fotocópia
Id: biblio-1171802
Autor: Chiapparrone María L; Morán Pedro E; Echevarría Hilda M; Soto Pedro; Paolicchi Fernando A; Catena María.
Título: Campylobacter fetus subsp. venerealis adhesion to MDBK cells / Campylobacter fetus subsp. venerealis adhesion to MDBK cells.
Fonte: Rev. argent. microbiol;46(3):269-70, oct. 2014.
Idioma: es.
Descritores: Aderência Bacteriana
Campylobacter fetus/fisiologia
Células Epiteliais/microbiologia
-Animais
Bovinos
Campylobacter fetus/ultraestrutura
Flagelos/fisiologia
Flagelos/ultraestrutura
Linhagem Celular
Microscopia Eletrônica de Varredura
Rim
Tipo de Publ: Artigo de Revista
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes



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