Base de dados : LILACS
Pesquisa : G06.120 [Categoria DeCS]
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Id: lil-761223
Autor: Santana, DP; Ribeiro, TF; Ribeiro, EL; Aquino, GLB; Naves, PLF.
Título: Ação de chalconas contra a formação de biofilme de Candida albicans / Action of chalcones against biofilm formation of Candida albicans
Fonte: Rev. ciênc. farm. básica apl;36(1), mar. 2015. tab, graf, ilus.
Idioma: pt.
Resumo: Candida albicans é uma levedura oportunista que apresenta um conjunto de fatores de virulência que conferem uma maior patogenicidade a estes micro- organismos. Neste trabalho foram estudadas a expressão de determinados fatores de virulência de C. albicans isoladas de cavidade bucal, com ênfase na formação de biofilme, e a atividade antifúngica e o impacto de concentrações subinibitórias de derivados de chalconas na formação de biofilme por estes micro-organismos. Os compostos foram avaliados quanto à toxicidade pelo ensaio de letalidade de Artemia salina e determinação da dose letal a 50% (DL 50 ) ao micro-crustáceo. Nas chalconas sintetizadas, manteve-se o grupameno hidroxil no anel B e foram feitas modificações no anel A que resultaram em diferenças na toxicidade dos compostos variando de 714,3 a 1798,1?g/mL. As leveduras expressaram de forma variável os fatores de virulência com predomínio de alta produção de fosfolipases, aspartil proteinases e com formação do tubo germinativo em até duas horas. A formação de biofilmes foi categorizada em dois grupos distintos nos quais 16 (48%) leveduras foram consideradas como formadoras fracas e 17 (52%) como formadoras fortes. A susceptibilidade as chalconas foi > 1000?g/mL exceto para (E)-1-fenil-3-(4-hidroxifenil) prop-2-em-1-ona que apresentou uma concentração inibitória mínima de 62,5?g/mL para os isolados e de 250?g/mL para a cepa de C. albicans ATCC 10231. Entretanto, concentrações subinibitórias das chalconas apresentaram uma significativa atividade inibitória da formação de biofilme por C. albicans, com graus de inibição variando de 75 a 90%, o que indica a potencial utilização destes compostos na inibição deste importante fator de virulência.(AU)

Candida albicans is an opportunistic yeast that has a number of virulence factors that increase pathogenicity of these micro-organisms. In this study the expression of certain virulence factors were studied of C. albicans isolated from the oral cavity with emphasis on biofilm formation and antifungal activity and the impact of subinibitory concentrations of derivatives of chalconas in biofilm formation by these micro-organisms. The compounds were evaluated for toxicity testing of brine shrimp lethality and determination of 50% lethal dose (LD50) to the micro-crustacean. In synthesized chalconas, remained grouping the hydroxyl in ring B and ring modifications were made on the resulting differences in toxicity of the compounds ranging from 714.3 to 1798.1 ?g/mL. Yeasts shown variable expressed virulence factors with prevalence of high production of phospholipases, aspartyl proteases and formation of germ tube within two hours. The biofilm formation was categorized into two distinct groups in which 16 (48%) yeasts were considered as poor and 17 trainers (52%) as forming strong. Susceptibility was chalcones ? 1000 ?g/mL except for (E)-1-phenyl-3-(4-hydroxyphenyl) prop-2-en-1-one, which showed a minimum inhibitory concentration of 62.5?g/mL for the clinical isolates and 250?g/mL for the strain of C. albicans ATCC 10231. However, subinibitory concentrations of chalcones showed significant inhibitory activity of biofilm formation by C. albicans with degrees of inhibition ranging from 75 to 90%, indicating the potential use of these compounds in the inhibition of this important virulence factor.(AU)
Descritores: Candida albicans/virologia
Biofilmes
Fatores de Virulência
Chalconas
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-1146814
Autor: De la Cruz Cardozo, Dolores; Pérez Madrigal, Evelyn Nineth; Pérez Sotelo, Angélica Cassandra; Cervantes Sandoval, Armando.
Título: Eficacia del uso de un enjuague con aceites esenciales para el control del biofilm dental / Efficacy of the use of a rinse with essential oils for the control of dental biofilm
Fonte: Rev. ADM = ADM;77(5):247-251, sept.-oct. 2020. tab, graf.
Idioma: es.
Resumo: Introducción: Se ha documentado que, para tener mejores resultados en el control del biofilm oral, es necesario el uso de controles químicos, antes o después del cepillado dental. Entre éstos, encontramos los enjuagues de aceites esenciales. Objetivo: Determinar la actividad del enjuague con aceites esenciales antes o después del cepillado, en el control del biofilm dental. Material y métodos: El estudio se realizó con 27 voluntarios. Los participantes fueron seleccionados y agrupados. El grupo 1 fue el control, que empleó su técnica de cepillado habitual y dos grupos experimentales que, además del cepillado con dentífrico, usaron un enjuague de aceites esenciales durante ocho días, ya sea antes (grupo 2) o después del cepillado dental (grupo 3). La cuantificación del número de superficies teñidas en los tres grupos, tanto el estado inicial como posterior al uso de los enjuagues, se hizo con el índice de O'Leary y un revelador de placa tritonal, el cual permitió la observación del biofilm de forma clínica, así como su grado de patogenicidad. Resultados y conclusión: El análisis estadístico estableció que no existe diferencia significativa entre no usar y usar el enjuague con aceites esenciales antes o después del cepillado (AU)

Introduction: It has been documented that, to have better results in the control of oral biofilm, it is necessary to use chemical control, before or after tooth brushing. Among these, we find the essential oil rinses. Objective: To determine the activity of the rinse with essential oils before or after brushing, in the control of the dental biofilm. Material and methods: The study was conducted with 27 volunteers. The participants were selected and grouped. Group 1 was the control, which used its usual brushing technique and two experimental groups that, in addition to brushing with toothpaste, used a mouthwash of essential oils, for eight days, either before (group 2) or after tooth brushing (group number 3). For the quantification of the number of stained surfaces in the three groups, to record both the initial and subsequent state of the use of the rinses, it was done with the O'Leary index and a tritonal developer, which allowed the observation of the biofilm of clinical form, as well as its degree of pathogenicity. Results and conclusion: The statistical analysis established that there is no significant difference between not using and using the rinse with essential oils before or after brushing (AU)
Descritores: Óleos Voláteis
Biofilmes
Antissépticos Bucais/uso terapêutico
-Escovação Dentária
Índice de Higiene Oral
Análise Estatística
Estudos Prospectivos
Análise de Variância
Estudos Longitudinais
Placa Dentária
México
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Tipo de Publ: Estudo Comparativo
Responsável: AR29.1 - Biblioteca


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Id: biblio-828121
Autor: Sun, Erlin; Liang, Gehong; Wang, Lining; Wei, Wenjie; Lei, Mingde; Song, Shiduo; Han, Ruifa; Wang, Yubao; Qi, Wei.
Título: Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms
Fonte: Braz. j. infect. dis;20(4):365-373, July-Aug. 2016. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract Aims We sought to characterize the antibiotic susceptibility of strains of Stenotrophomonas maltophilia isolated from clinical samples, and the role of Stenotrophomonas maltophilia biofilm in antibiotic resistance. Methods Fifty-one clinical Stenotrophomonas maltophilia isolates were obtained from patients with nosocomial infection in the surgical wards and ICUs of six general hospitals in Tianjin, China. In vitro models of Stenotrophomonas maltophilia biofilms were established and confirmed by scanning electron microscopy and fluorescence microscopy with silver staining. The minimal inhibitory concentrations and biofilm inhibitory concentrations of commonly used antibiotics were determined. Results 47 of 51 strains were resistant to three or more antibiotics. 42 of 51 strains formed Stenotrophomonas maltophilia biofilms in vitro. Stenotrophomonas maltophilia biofilm formation greatly reduced sensitivity to most tested antibiotics, but not to levofloxacin. However, in the presence of erythromycin scanning electron microscopy revealed that levofloxacin inhibited Stenotrophomonas maltophilia biofilm formation. Factorial ANOVA revealed that erythromycin enhanced susceptibility to levofloxacin, cefoperazone/sulbactam, and piperacillin (p < 0.05), and an ΔE model revealed that levofloxacin and erythromycin acted synergistically in biofilms, suggesting specific use of combined macrolide therapy may represent an effective treatment for Stenotrophomonas maltophilia infection. Conclusions Antibiotics could act synergistically to combat the protection conferred to clinical isolates of Stenotrophomonas maltophilia by biofilms. Macrolide antibiotics may be effective where used in combination.
Descritores: Biofilmes/crescimento & desenvolvimento
Stenotrophomonas maltophilia/efeitos dos fármacos
Antibacterianos/farmacologia
-Testes de Sensibilidade Microbiana
Infecção Hospitalar/microbiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Stenotrophomonas maltophilia/isolamento & purificação
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-828136
Autor: Martínez-Meléndez, Adrián; Morfín-Otero, Rayo; Villarreal-Treviño, Licet; Camacho-Ortíz, Adrián; González-González, Gloria; Llaca-Díaz, Jorge; Rodríguez-Noriega, Eduardo; Garza-González, Elvira.
Título: Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23
Fonte: Braz. j. infect. dis;20(5):419-428, Sept.-Oct. 2016. tab, graf.
Idioma: en.
Projeto: CONACyT (Mexican Council for Science and Technology).
Resumo: Abstract The mechanisms contributing to persistence of coagulase-negative staphylococci are diverse; to better understanding of their dynamics, the characterization of nosocomial isolates is needed. Our aim was to characterize phenotypic and molecular characteristics of Staphylococcus epidermidis and Staphylococcus haemolyticus human blood isolates from two tertiary care hospitals in Mexico, the Hospital Universitario in Monterrey and the Hospital Civil in Guadalajara. Antimicrobial susceptibility was determined. Biofilm formation was assessed by crystal violet staining. Detection of the ica operon and Staphylococcal Cassette Chromosome mec typing were performed by PCR. Clonal relatedness was determined by Pulsed-fiel gel electrophoresis and Multi locus sequence typing. Methicillin-resistance was 85.5% and 93.2% for S. epidermidis and S. haemolyticus, respectively. Both species showed resistance >70% to norfloxacin, clindamycin, levofloxacin, trimethoprim/sulfamethoxazole, and erythromycin. Three S. epidermidis and two S. haemolyticus isolates were linezolid-resistant (one isolate of each species was cfr+). Most isolates of both species were strong biofilm producers (92.8% of S. epidermidis and 72.9% of S. haemolyticus). The ica operon was amplified in 36 (43.4%) S. epidermidis isolates. SCCmec type IV was found in 47.2% of the S. epidermidis isolates and SCCmec type V in 14.5% of S. haemolyticus isolates. No clonal relatedness was found in either species. Resistance to clindamycin, levofloxacin, erythromycin, oxacillin, and cefoxitin was associated with biofilm production for both species (p < 0.05). A G2576T mutation in 23S rRNA gene was detected in an S. haemolyticus linezolid-resistant isolate. All linezolid-resistant S. epidermidis isolates belonged to ST23; isolate with SCCmec type IV belonged to ST7, and isolate with SCCmec type III belonged to ST2. This is the first report of ST7 in Mexico. There was a high genetic diversity in both species, though both species shared characteristics that may contibute to virulence.
Descritores: Staphylococcus epidermidis/isolamento & purificação
Staphylococcus epidermidis/efeitos dos fármacos
Coagulase/sangue
Staphylococcus haemolyticus/efeitos dos fármacos
Linezolida/farmacologia
Antibacterianos/farmacologia
-Valores de Referência
Staphylococcus epidermidis/genética
DNA Bacteriano
Testes de Sensibilidade Microbiana
Eletroforese em Gel de Campo Pulsado
Coagulase/isolamento & purificação
Coagulase/genética
Biofilmes/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
Farmacorresistência Bacteriana
Staphylococcus haemolyticus/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Tipagem de Sequências Multilocus
Reação em Cadeia da Polimerase Multiplex
México
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo Multicêntrico
Responsável: BR1.1 - BIREME


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Id: biblio-828141
Autor: Ferreira, Thiago de Oliveira; Koto, Rafael Yoshio; Leite, Gabriel Fialkovitz da Costa; Klautau, Giselle Burlamaqui; Nigro, Stanley; Silva, Cely Barreto da; Souza, Ana Paula Idalgo da Fonseca; Mimica, Marcelo Jenne; Cesar, Regina Grigolli; Salles, Mauro José Costa.
Título: Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients
Fonte: Braz. j. infect. dis;20(5):468-475, Sept.-Oct. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Objectives To compare cultured microorganisms identified on endotracheal tubes biofilms through sonication technique with traditional tracheal aspirate collected at extubation of pediatric intensive care unit patients. Methods Demographic and epidemiological data were analyzed to identify factors possibly related with the microbiological profile of the two collection methods. Associations between categorical and continuous variables were analyzed using the chi-square or Fisher's exact test, or Student's t test. p-Value <0.05 were considered significant. Results Thirty endotracheal tubes and tracheal aspirates samples from 27 subjects were analyzed. Only one patient presented the clinical diagnosis of ventilator-associated pneumonia. Overall, 50% of bacteria were Gram-negative bacilli, followed by Gram-positive bacteria in 37%, and fungi in 10%. No statistically significant difference on the distribution of Gram-positive or Gram-negative bacteria (p = 0.996), and fungi (p = 0.985) were observed between the collection methods. Pseudomonas spp. was the most frequent microorganism identified (23.8%), followed by Streptococcus spp. (18.5%), Acinetobacter spp. (15.9%), coagulase-negative staphylococci (11.2%), and Klebsiella spp. (8.6%). Concordant results between methods amounted to 83.3%. Pseudomonas aeruginosa and Acinetobacter baumannii showed carbapenem resistance in 50% and 43.7% of the isolates, respectively. In general, cultures after endotracheal tubes sonication (non-centrifuged sonication fluid and centrifuged sonication fluid) yielded bacteria with higher rates of antimicrobial resistance compared to tracheal aspirates cultures. Additionally, in 12 subjects (40%), we observed discrepancies regarding microbiologic profiles of cultures performed using the collection methods. Conclusions Our study demonstrated that sonication technique can be applied to ET biofilms to identify microorganisms attached to their surface with a great variety of species identified. However, we did not find significant differences in comparison with the traditional tracheal aspirate culture approach.
Descritores: Sonicação/métodos
Unidades de Terapia Intensiva Pediátrica/estatística & dados numéricos
Biofilmes/crescimento & desenvolvimento
Equipamentos e Provisões Hospitalares/microbiologia
Intubação Intratraqueal/instrumentação
-Valores de Referência
Fatores de Tempo
Traqueia/microbiologia
Contagem de Colônia Microbiana
Testes de Sensibilidade Microbiana
Contaminação de Equipamentos/estatística & dados numéricos
Reprodutibilidade dos Testes
Pneumonia Associada à Ventilação Mecânica/microbiologia
Fungos/isolamento & purificação
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Positivas/isolamento & purificação
Tempo de Internação
Antibacterianos/uso terapêutico
Limites: Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Responsável: BR1.1 - BIREME


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Id: biblio-828168
Autor: Pal, Rahul; Hameed, Saif; Sharma, Sharda; Fatima, Zeeshan.
Título: Influence of iron deprivation on virulence traits of mycobacteria
Fonte: Braz. j. infect. dis;20(6):585-591, Nov.-Dec. 2016. graf.
Idioma: en.
Resumo: ABSTRACT Novel strategies to combat the ever increasing burden of drug resistance in Mycobacterium tuberculosis (MTB) causing tuberculosis (TB) remains a global concern. The ability of MTB to sense and adapt to restricted iron conditions in the hostile environment is essential for their survival and confers the basis of their success as dreadful pathogen. The striking and clinically relevant virulence trait of MTB is its ability to form biofilms and adhere to the host cells. The present study elucidated the effect of iron deprivation on biofilm formation and cell adherence of Mycobacterium smegmatis, a non-pathogenic surrogate of MTB. Firstly, we showed that iron deprivation leads to enhanced cell sedimentation rate and altered colony morphology depicting alterations in cell surface envelope properties. We explored that biofilm formation and cell adherence to polystyrene surface as well as human oral epithelial cells were considerably reduced under iron deprivation both in presence of 2,2 BP (iron chelator) and siderophore mutant Δ011-14 strain. We further investigated that the potency of three first line anti-TB drugs (Isoniazid, Ethambutol, Rifampicin) to inhibit both biofilm formation and cell adhesion were enhanced under iron deprivation in contrast to the drugs when tested alone. Taken together, by virtue of the indispensability of iron for functional virulence traits in mycobacteria, iron deprivation strategies could be further exploited against this notorious human pathogen to explore novel drug targets.
Descritores: Virulência
Aderência Bacteriana/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Mycobacterium smegmatis/patogenicidade
Células Epiteliais/microbiologia
Ferro/farmacologia
-Biofilmes/efeitos dos fármacos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-839179
Autor: Zmantar, Tarek; Ben Slama, Rihab; Fdhila, Kais; Kouidhi, Bochra; Bakhrouf, Amina; Chaieb, Kamel.
Título: Modulation of drug resistance and biofilm formation of Staphylococcus aureus isolated from the oral cavity of Tunisian children
Fonte: Braz. j. infect. dis;21(1):27-34, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Objectives: This study aims to investigate the antimicrobial and the anti-biofilm activities of Lactobacillus plantarum extract (LPE) against a panel of oral Staphylococcus aureus (n = 9) and S. aureus ATCC 25923. The in vitro ability of LPE to modulate bacterial resistance to tetracycline, benzalchonium chloride, and chlorhexidine were tested also. Methods: The minimum inhibitory concentrations (MICs) and the minimal bactericidal concentrations of Lactobacillus plantarum extract, tetracycline, benzalchonium chloride and clohrhexidine were determined in absence and in presence of a sub-MIC doses of LPE (1/2 MIC). In addition, the LPE potential to inhibit biofilm formation was assessed by microtiter plate and atomic force microscopy assays. Statistical analysis was performed on SPSS v. 17.0 software using Friedman test and Wilcoxon signed ranks test. These tests were used to assess inter-group difference (p < 0.05). Results: Our results revealed that LPE exhibited a significant antimicrobial and anti-biofilm activities against the tested strains. A synergistic effect of LPEs and drug susceptibility was observed with a 2–8-fold reduction. Conclusion: LPE may be considered to have resistance-modifying activity. A more detailed investigation is necessary to determine the active compound responsible for therapeutic and disinfectant modulation.
Descritores: Staphylococcus aureus/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Farmacorresistência Bacteriana/efeitos dos fármacos
Antibacterianos/farmacologia
Boca/microbiologia
-Valores de Referência
Tetraciclina/farmacologia
Compostos de Benzalcônio/farmacologia
Testes de Sensibilidade Microbiana
Clorexidina/farmacologia
Reação em Cadeia da Polimerase
Reprodutibilidade dos Testes
Estatísticas não Paramétricas
Microscopia de Força Atômica/métodos
Biofilmes/efeitos dos fármacos
Lactobacillus plantarum/química
Anti-Infecciosos Locais/farmacologia
Limites: Humanos
Criança
Responsável: BR1.1 - BIREME


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Timm, Claudio Dias
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Id: biblio-1118084
Autor: Gonçalves, Thaís Gonçalves; Timm, Cláudio Dias.
Título: Biofilm production by coagulase-negative Staphylococcus: a review / Produção de biofilme por Staphylococcus coagulase-negativo: uma revisão
Fonte: Arq. Inst. Biol;87:e1382018, 2020.
Idioma: en.
Resumo: This review aimed to describe the biofilm formation ability of coagulase-negative Staphylococcus, addressing its impact to the food industry. Coagulase-negative Staphylococcus have the ability to produce enterotoxins in food, making it an important line of study, as it constitutes a risk to public health. The biofilm formation by these microorganisms requires physicochemical processes, such as hydrophobic forces, which are essential for the first phase of fixing the biofilm on the surface. In industrial facilities, stainless steel equipment is the most associated with the formation of biofilms, due to the presence grooves and cracks. Many species of coagulase-negative Staphylococcus produce biofilm, but the most studied is S. epidermidis, as it is the most frequently isolated from food. Coagulase-negative Staphylococcus form biofilm on different surfaces in the food industry, and can become a source of permanent contamination, that can be present in the final product, intended for human consumption. Among other alternatives to combat the formation of biofilm in industrial food facilities, there is the implementation of Good Manufacturing Practices, which is effective in preventing bacterial adhesion, and therefore, the formation of biofilm. However, further studies are needed in order to quantify the occurrence of coagulase-negative Staphylococcus biofilms in the food industry.(AU)

Esta revisão bibliográfica teve como objetivo descrever a capacidade de formação de biofilme por Staphylococcus coagulase-negativo, relacionando seu impacto na indústria alimentícia. Os Staphylococcus coagulase-negativos possuem a capacidade de produzir enterotoxina nos alimentos, tornando-se uma importante linha de estudo, pois constitui um risco para a saúde pública. A formação do biofilme por esses micro-organismos requer processos físico-químicos, como forças hidrofóbicas, essenciais para a primeira fase de fixação do biofilme na superfície. Nas indústrias, equipamentos de aço inoxidável são os mais associados à formação de biofilmes, em decorrência de possuírem ranhuras e fendas. Muitas espécies de Staphylococcus coagulase-negativo produzem biofilme, porém, o mais estudado é o S. epidermidis, por ser o mais frequentemente isolado de alimentos. Os Staphylococcus coagulase-negativos formam biofilme em diferentes superfícies de indústrias alimentícias, podendo se tornar uma fonte de contaminação permanente, contaminando o produto final destinado ao consumo humano. Dentre outras alternativas para combater a formação do biofilme nas plantas alimentícias, a implantação das Boas Práticas de Fabricação é eficaz para prevenir a adesão bacteriana, evitando a formação do biofilme. No entanto, são necessários estudos para quantificar a ocorrência de biofilmes de Staphylococcus coagulase-negativos em indústrias alimentícias.(AU)
Descritores: Staphylococcus/fisiologia
Coagulase
Biofilmes/crescimento & desenvolvimento
-Contaminação de Alimentos
Indústria Alimentícia
Tipo de Publ: Revisão
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Timenetsky, Jorge
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Id: biblio-1039197
Autor: Carvalho, Suzi P de; Almeida, Jéssica B de; Andrade, Yasmin M F S; Silva, Lucas S C da; Oliveira, Arianne C de; Nascimento, Flávia S; Campos, Guilherme B; Oliveira, Márcio V; Timenetsky, Jorge; Marques, Lucas M.
Título: Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil
Fonte: Braz. j. infect. dis;21(4):464-467, July-Aug. 2017. tab.
Idioma: en.
Resumo: Abstract Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three clonal complexes (CC): 5, 45, and 398. Four spa types were found circulating in this setting. Resistance of S. aureus isolates to ampicillin, erythromycin, ciprofloxacin, clindamycin, and tetracycline was 80%, 32.8%, 7.1%, 7.1% and 4.3%, respectively. One isolate presented intermediate resistance to vancomycin detected by Etest methodology. All strains were biofilm producers. The virulence genes seb, sec, spa, and pvl were detected in some isolates. This study revealed a high rate of children carrying MRSA among healthy attendees in daycare centers in Vitória da Conquista, Brazil.
Descritores: Infecções Estafilocócicas/microbiologia
Creches
Nariz/microbiologia
-Infecções Comunitárias Adquiridas/microbiologia
Biofilmes/crescimento & desenvolvimento
Fatores de Virulência
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/patogenicidade
Genótipo
Antibacterianos/farmacologia
Limites: Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Responsável: BR1.1 - BIREME


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Id: biblio-951633
Autor: Lima, Jailton Lobo da Costa; Alves, Lilian Rodrigues; Jacomé, Paula Regina Luna de Araújo; Bezerra Neto, João Pacífico; Maciel, Maria Amélia Vieira; Morais, Marcia Maria Camargo de.
Título: Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing
Fonte: Braz. j. infect. dis;22(2):129-136, Mar.-Apr. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Introduction: Biofilm production is an important mechanism for the survival of Pseudomonas aeruginosa and its relationship with antimicrobial resistance represents a challenge for patient therapeutics. P. aeruginosa is an opportunistic pathogen frequently associated to nosocomial infections, especially in imunocompromised hosts. Objectives: Analyze the phenotypic biofilm production in P. aeruginosa isolates, describe clonal profiles, and analyze quorum sensing (QS) genes and the occurrence of mutations in the LasR protein of non-biofilm producing isolates. Methods: Isolates were tested for biofilm production by measuring cells adherence to the microtiter plates. Clonal profile analysis was carried out through ERIC-PCR, QS genes were by specific PCR. Results: The results showed that 77.5% of the isolates were considered biofilm producers. The results of genotyping showed 38 distinct genetic profiles. As for the occurrence of the genes, 100% of the isolates presented the lasR, rhlI and rhlR genes, and 97.5%, presented the lasI gene. In this study nine isolates were not biofilm producers. However, all presented the QS genes. Amplicons related to genes were sequenced in three of the nine non-biofilm-producing isolates (all presenting different genetic similarity profile) and aligned to the sequences of those genes in P. aeruginosa strain PAO1 (standard biofilm-producing strain). Alignment analysis showed an insertion of three nucleotides (T, C and G) causing the addition of an amino acid valine in the sequence of the LasR protein, in position 53. Conclusion: The modeling of the resulting LasR protein showed a conformational change in its structure, suggesting that this might be the reason why these isolates are unable to produce biofilm.
Descritores: Pseudomonas aeruginosa/fisiologia
Infecções por Pseudomonas/microbiologia
Proteínas de Bactérias/genética
Transativadores/genética
Biofilmes/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
-Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/química
Infecções por Pseudomonas/tratamento farmacológico
Proteínas de Bactérias/química
Transativadores/química
Reação em Cadeia da Polimerase/métodos
Infecção Hospitalar
Farmacorresistência Bacteriana Múltipla
Anti-Infecciosos/farmacologia
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME



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