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Pesquisa : G06.550.800 [Categoria DeCS]
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Id: biblio-1047367
Autor: Qureshi, M. Amjad; Tariq Pervez, Muhammad; Ellahi Babar, Masroor; Hussain, Tanveer; Shoaib, Muhammad; Shah Mohammad, Syed.
Título: Genomic comparisons of Rhizobium species using in silico AFLP-PCR, endonuclease restriction, and AMPylating enzymes
Fonte: Electron. j. biotechnol;34:67-75, july. 2018. graf, tab.
Idioma: en.
Resumo: Background: The whole-genome sequences of nine Rhizobium species were evaluated using different in silico molecular techniques such as AFLP-PCR, restriction digest, and AMPylating enzymes. The entire genome sequences were aligned with progressiveMauve and visualized by reconstructing phylogenetic tree using NTSYS pc 2.11X. The "insilico.ehu.es" was used to carry out in silico AFLP-PCR and in silico restriction digest of the selected genomes. Post-translational modification (PTM) and AMPylating enzyme diversity between the proteome of Rhizobium species were determined by novPTMenzy. Results: Slight variations were observed in the phylogeny based on AFLP-PCR and PFGE and the tree based on whole genome. Results clearly demonstrated the presence of PTMs, i.e., AMPylation with the GS-ATasE (GlnE), Hydroxylation, Sulfation with their domain, and Deamidation with their specific domains (AMPylating enzymes) GS-ATasE (GlnE), Fic, and Doc (Phosphorylation); Asparagine_hydroxylase and Collagen_prolyl_lysyl_hydroxylase; Sulfotransferase; and CNF (Cytotoxic Necrotizing Factors), respectively. The results pertaining to PTMs are discussed with regard to functional diversities reported in these species. Conclusions: The phylogenetic tree based on AFLP-PCR was slightly different from restriction endonuclease- and PFGE-based trees. Different PTMs were observed in the Rhizobium species, and the most prevailing type of PTM was AMPylation with the domain GS-ATasE (GlnE). Another type of PTM was also observed, i.e., Hydroxylation and Sulfation, with the domains Asparagine_hydroxylase and Collagen_prolyl_lysyl_hydroxylase and Sulfotransferase, respectively. The deamidation type of PTM was present only in Rhizobium sp. NGR234. How to cite: Qureshi MA, Pervez MT, Babar ME, et al. Genomic comparisons of Rhizobium species using in silico AFLP-PCR, endonuclease restrictions and ampylating enzymes.
Descritores: Rhizobium/genética
-Filogenia
Rhizobium/enzimologia
Rhizobium/fisiologia
Simbiose
Simulação por Computador
Enzimas de Restrição do DNA
Reação em Cadeia da Polimerase/métodos
Análise de Sequência
Proteoma
Genômica
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Fabaceae/microbiologia
Responsável: CL1.1 - Biblioteca Central


  2 / 92 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-1041408
Autor: Alencar, Ronildo Baiatone; Saraiva, José Ferreira; Oliveira, Arley Faria José de; Scarpassa, Vera Margarete.
Título: First record of anopheles konderi Galvão & Damasceno (Diptera: Culicidae) carrying eggs of dermatobia hominis (Linnaeus Jr. ) (Diptera: oestridae), from oriximiná municipality, Pará, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;50(3):388-390, May-June 2017. graf.
Idioma: en.
Projeto: Ministério da Ciência, Tecnologia, Inovações e Comunicações.
Resumo: Abstract INTRODUCTION: The muscoid fly Dermatobia hominis causes cutaneous myiases in mammals. Females of this species use a vector to carry their eggs to the host. This note describes Anopheles konderi acting as phoretic vector for D. hominis. METHODS: A female A. konderi carrying D. hominis was collected using light traps in Oriximiná, Pará, Brazil. The A. konderi specimen was identified at morphological and molecular levels. RESULTS: Eight eggs of D. hominis were observed on the Anopheles konderi female. CONCLUSIONS: Anopheles konderi, only the third Anopheles species recorded as a phoretic vector, may be a potential vector of D. hominis.
Descritores: Anopheles/parasitologia
-Óvulo
Simbiose
Brasil
Dípteros/crescimento & desenvolvimento
Anopheles/classificação
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


  3 / 92 LILACS  
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Id: biblio-950763
Autor: O'Connor-Sánchez, Aileen; Rivera-Domínguez, Adán J; De los Santos-Briones, César; López-Aguiar, Lluvia K; Peña-Ramírez, Yuri J; Prieto-Davo, Alejandra.
Título: Acidobacteria appear to dominate the microbiome of two sympatric Caribbean Sponges and one Zoanthid
Fonte: Biol. Res;47:1-6, 2014. graf, tab.
Idioma: en.
Projeto: CICY Fiscal Fund; . FOMIX-Yucatán; . CONACYT; . UNAM. Facultad de Química.
Resumo: BACKGROUND: Marine invertebrate-associated microbial communities are interesting examples of complex symbiotic systems and are a potential source of biotechnological products. RESULTS: In this work, pyrosequencing-based assessment from bacterial community structures of sediments, two sponges, and one zoanthid collected in the Mexican Caribbean was performed. The results suggest that the bacterial diversity at the species level is higher in the sediments than in the animal samples. Analysis of bacterial communities' structure showed that about two thirds of the bacterial diversity in all the samples belongs to the phyla Acidobacteria and Proteobacteria. The genus Acidobacteriumappears to dominate the bacterial community in all the samples, reaching almost 80% in the sponge Hyrtios. CONCLUSIONS: Our evidence suggests that the sympatric location of these benthonic species may lead to common bacterial structure features among their bacterial communities. The results may serve as a first insight to formulate hypotheses that lead to more extensive studies of sessile marine organisms' microbiomes from the Mexican Caribbean.
Descritores: Poríferos/microbiologia
Antozoários/microbiologia
Acidobacteria/fisiologia
Simpatria
Microbiota/fisiologia
-Filogenia
Poríferos/classificação
Simbiose/fisiologia
RNA Ribossômico 16S/análise
Região do Caribe
Sedimentos Geológicos/microbiologia
Proteobactérias/classificação
Proteobactérias/fisiologia
Antozoários/classificação
Biodiversidade
México
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1171774
Autor: Spagnoletti Federico N; Fernandez di Pardo Agustina; Tobar Gómez Natalia E; Chiocchio Viviana M.
Título: Las micorrizas arbusculares y Rhizobium: una simbiosis dual de interés / [Arbuscular mycorrhizae and Rhizobium: a dual symbiosis of interest].
Fonte: Rev. argent. microbiol;45(2):131-2, jun. 2013.
Idioma: es.
Descritores: Micorrizas
Rhizobium
-Rhizobium/fisiologia
Simbiose
Tipo de Publ: Artigo de Revista
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


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Texto completo SciELO Brasil
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Id: biblio-1059207
Autor: Leonidas, Carolina; Santos, Manoel Antônio dos.
Título: Ilusão simbiótica e construção da identidade feminina nos transtornos alimentares: perspectiva da psicossomática psicanalítica / Symbiotic illusion and female identity construction in eating disorders: a psychoanalytical psychosomatics' perspective
Fonte: Ágora (Rio J. Online);23(1):84-93, Jan.-Apr. 2020.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract: This study aims primarily to reflect on the role of symbiotic illusion in the construction of female identity in women with eating disorders (ED), using premises from psychoanalytic psychosomatics. Considering ED as psychopathologies related to the affective economy, Oedipal relations play crucial role in the process of identity constitution. Mother-daughter relationships in ED are shaped in the molds of symbiotic illusion, with weak father figures. We presented a case study to briefly illustrate the theoretical framework. The omissive posture of a father to interdict the symbiotic relation leads daughters to find themselves unable to libidinally invest in other objects.

Resumo: Este estudo teve como objetivo refletir sobre o papel da ilusão simbiótica na construção da identidade feminina em mulheres com transtornos alimentares (TAs), utilizando as premissas da psicossomática psicanalítica. Considerando-se os TAs como psicopatologias relacionadas à economia afetiva, as relações edípicas desempenham papel crucial no processo de construção de identidade. As relações mãe-filha nos TAs são moldadas pela ilusão simbiótica, com figuras paternas enfraquecidas. Nós apresentamos brevemente um estudo de caso para ilustrar os conceitos do referencial teórico. A postura omissa do pai para interditar a relação simbiótica com a mãe leva a filha à impossibilidade de investir libidinalmente em outros objetos.
Descritores: Transtornos Psicofisiológicos/psicologia
Identificação Social
Mulheres/psicologia
Transtornos da Alimentação e da Ingestão de Alimentos/psicologia
-Relações Pais-Filho
Simbiose
Anorexia Nervosa/psicologia
Libido
Limites: Humanos
Feminino
Adulto
Adulto Jovem
Tipo de Publ: Relatos de Casos
Responsável: BR1194.1 - BT - Biblioteca


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Id: biblio-1053231
Autor: Sánchez-Tafolla, Leonardo; Padrón, José M; Mendoza, Guillermo; Luna-Rodríguez, Mauricio; Fernández, José J; Norte, Manuel; Trigos, Ángel.
Título: Antiproliferative activity of biomass extract from Pseudomonas cedrina
Fonte: Electron. j. biotechnol;40:40-44, July. 2019. tab, ilus.
Idioma: en.
Projeto: Consejo Nacional de Ciencia y Tecnología (CONACYT).
Resumo: Background: The study of plant-associated microorganisms is very important in the discovery and development of bioactive compounds. Pseudomonas is a diverse genus of Gammaproteobacteria comprising more than 60 species capable of establishing themselves in many habitats, which include leaves and stems of many plants. There are reports of metabolites with diverse biological activity obtained from bacteria of this genus, and some of the metabolites have shown cytotoxic activity against cancer cell lines. Because of the high incidence of cancer, research in recent years has focused on obtaining new sources of active compounds that exhibit interesting pharmacodynamic and pharmacokinetic properties that lead to the development of new therapeutic agents. Results: A bacterial strain was isolated from tumors located in the stem of Pinus patula, and it was identified as Pseudomonas cedrina. Extracts from biomass and broth of P. cedrina were obtained with chloroform:methanol (1:1). Only biomass extracts exhibited antiproliferative activity against human tumor cell lines of cervix (HeLa), lung (A-549), and breast (HBL-100). In addition, a biomass extract from P. cedrina was fractioned by silica gel column chromatography and two diketopiperazines were isolated: cyclo-(L-Prolyl-L-Valine) and cyclo-(L-Leucyl-L-Proline). Conclusions: This is the first report on the association of P. cedrina with the stems of P. patula in Mexico and the antiproliferative activity of extracts from this species of bacteria against human solid tumor cell lines.
Descritores: Pseudomonas/química
Pinus/microbiologia
Linhagem Celular Tumoral/efeitos dos fármacos
Antineoplásicos/farmacologia
-Plantas/microbiologia
Simbiose
Biomassa
Gammaproteobacteria/química
Proliferação de Células/efeitos dos fármacos
Responsável: CL1.1 - Biblioteca Central


  7 / 92 LILACS  
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Id: biblio-1003280
Autor: Pérez-Peralta, Paulina Janneth; Ferrera-Cerratoa, Ronald; Alarcón, Alejandro; Trejo-Téllez, Libia I; Cruz-Ortega, Rocío; Silva-Rojas, Hilda V.
Título: Respuesta del simbiosistema frijol (Phaseolus vulgaris L.) y Rhizobium tropici CIAT899 ante el efecto alelopático de Ipomoea purpurea L. Roth / Responses of the common bean (Phaseolus vulgaris L.) and Rhizobium tropici CIAT899 symbiosystem to induced allelopathy by Ipomoea purpurea L. Roth
Fonte: Rev. argent. microbiol;51(1):47-55, mar. 2019. graf, tab.
Idioma: es.
Resumo: La alelopatía es un fenómeno que involucra la producción de metabolitos secundarios que influyen en el crecimiento de las plantas, pero este potencial alelopático ha sido poco estudiado en la simbiosis rizobio-leguminosa. Esta investigación tuvo los siguientes objetivos: 1) evaluar el potencial alelopático de lixiviados acuosos de Ipomoea purpurea L. Roth en la germinación de semillas y en el crecimiento radical de plántulas de frijol (Phaseolus vulgaris L.); 2) determinar el efecto de estos lixiviados en el crecimiento in vitro de Rhizobium tropici CIAT899, y 3) evaluar el potencial alelopático de I. purpurea en el crecimiento, la fisiología y la nodulación de frijol en simbiosis con R. tropici. Tanto el lixiviado acuoso de raíz como el de la parte aérea de I. purpurea estimularon la germinación de semillas de frijol y la elongación radical. El crecimiento in vitro de R. tropici fue inhibido al aplicar los 2 tipos de lixiviado. La presencia de I. purpurea tuvo un efecto negativo en el crecimiento y en las respuestas fisiológicas de las plantas de frijol, que fue atenuado cuando las plantas fueron inoculadas con Rhizobium tropici; no obstante, la nodulación asociada a esta bacteria fue afectada en presencia de la planta alelopática. Los resultados indican que la simbiosis de rizobios en las raíces de frijol es un elemento importante en la atenuación de los danos producidos por la planta alelopática I. purpurea.

Allelopathy is a phenomenon that involves the production of secondary metabolites that influence the growth of plants and microorganisms; however, this alellopathic effect has been scarcely studied on the rhizobia-legume symbiosis. The aims of this research were 1) to assess the allelopathic potential of aqueous extracts of Ipomoea purpurea L. Roth on seed germination and root length of common bean seedlings (Phaseolus vulgaris L.), 2) to determine its effects on the in vitro growth of Rhizobium tropici CIAT899, and 3) to evaluate the allelopathic potential of I. purpurea on the growth, nodulation and physiology of common bean plants inoculated with R. tropici. After 48 h, 15% of the aqueous root extract of I. purpurea stimulated seed germination, whereas 4% of the aqueous shoot extracts stimulated such germination. Both the root or shoot extracts stimulated seed germination and e root length. In vitro growth of R. tropici was inhibited as a result of the application of both aqueous extracts. The presence of I. purpurea negatively affected both the growth and physiological responses of common bean plants, and this effect was attenuated after the inoculation of R. tropici; nevertheless, this allelopathic plant affected root nodulation. Our results suggest that the symbiosis of rhizobia and roots of common bean plants is an important element for attenuating the negative effects caused by the allelopathic plant.
Descritores: Simbiose/fisiologia
Ipomoea purpurea/análise
Phaseolus/crescimento & desenvolvimento
Rhizobium tropici/crescimento & desenvolvimento
Alelopatia/fisiologia
-Técnicas In Vitro/métodos
Fabaceae/fisiologia
Tipo de Publ: Estudo de Avaliação
Estudo Observacional
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


  8 / 92 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-886906
Autor: SILVA, GLÁUCIA ALVES E; SIQUEIRA, JOSÉ O; STÜRMER, SIDNEY L; MOREIRA, FATIMA M S.
Título: Effectiveness of Arbuscular Mycorrhizal Fungal Isolates from the Land Uses of Amazon Region in Symbiosis with Cowpea
Fonte: An. acad. bras. ciênc;90(1):357-371, Mar. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Arbuscular mycorrhizal fungi provide several ecosystem services, including increase in plant growth and nutrition. The occurrence, richness, and structure of arbuscular mycorrhizal fungi communities are influenced by human activities, which may affect the functional benefits of these components of the soil biota. In this study, 13 arbuscular mycorrhizal fungi isolates originating from soils with different land uses in the Alto Solimões-Amazon region were evaluated regarding their effect on growth, nutrition, and cowpea yield in controlled conditions using two soils. Comparisons with reference isolates and a mixture of isolates were also performed. Fungal isolates exhibited a wide variability associated with colonization, sporulation, production of aboveground biomass, nitrogen and phosphorus uptake, and grain yield, indicating high functional diversity within and among fungal species. A generalized effect of isolates in promoting phosphorus uptake, increase in biomass, and cowpea yield was observed in both soils. The isolates of Glomus were the most efficient and are promising isolates for practical inoculation programs. No relationship was found between the origin of fungal isolate (i.e. land use) and their symbiotic performance in cowpea.
Descritores: Solo/química
Microbiologia do Solo
Simbiose/fisiologia
Micorrizas/isolamento & purificação
Micorrizas/fisiologia
Vigna/crescimento & desenvolvimento
-Fósforo/análise
Fatores de Tempo
Brasil
Raízes de Plantas/microbiologia
Biodiversidade
Vigna/microbiologia
Nitrogênio/análise
Responsável: BR1.1 - BIREME


  9 / 92 LILACS  
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Id: biblio-1118852
Autor: KAMINSKI, Lucas A; CARNEIRO, Eduardo; DOLIBAINA, Diego R; CASAGRANDE, Mirna M; MIELKE, Olaf H H.
Título: Oviposition of Minstrellus grandis (Lepidoptera: Riodinidae) in a harmful ant-plant symbiosi
Fonte: Acta amaz;50(3):256-259, jul. - set. 2020.
Idioma: en.
Resumo: The oviposition behavior of the rare butterfly Minstrellus grandis (Callaghan, 1999) (Lepidoptera: Riodinidae) is recorded for the first time. Two females laid eggs on the old leaves of an unidentified Triplaris sp. (Polygonaceae), a myrmecophytic plant typically known as 'Triplaria' or 'novice' tree, inhabited by aggressive 'taxi' ants of the genus Pseudomyrmex sp. (Hymenoptera: Formicidae). These observations suggest that M. grandis caterpillars live associated with one of the most harmful types of Amazon ant-plant symbiosis. (AU)
Descritores: Formigas
Simbiose
Borboletas
Ecossistema Amazônico
Carnivoridade
-Oviposição
Responsável: BR6.1 - BCS - Biblioteca de Ciências da Saúde


  10 / 92 LILACS  
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Id: biblio-951563
Autor: Almeida, R P; Stouthamer, R.
Título: Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae) / Filogenia do endosimbionte Wolbachia em Trichogramma, an alpha-proteobacteria (Rickettsiae)
Fonte: Braz. j. biol;78(3):421-428, Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Wolbachia (Hertig) endosymbionts are extensively studied in a wide range of organisms and are known to be transmitted through the egg cytoplasm to the offsping. Wolbachia may cause several types of reproductive modifications in arthropods. In Trichogramma species, parthenogenesis-inducing Wolbachia bacteria allow females wasps to produce daughters from unfertilized eggs and these bacteria are present in at least 9% of all Trichogramma species. Phylogenetic studies have led to the subdivision of the Wolbachia clade in five supergroups (A, B, C, D and E) and Wolbachia from Trichogramma belong to supergroup B. Here, using the wsp gene, four groups of Wolbachia that infect Trichogramma species were distinguished and the addition of a new group "Ato" was suggested due to the addition of Wolbachia from Trichogramma atopovirilia (Oatman and Platner). Specific primers were designed and tested for the "Ato" group. Seventy-five percent of all evaluated Wolbachia strains from Trichogramma fell within "Sib" group.

Resumo Endosimbiontes do gênero Wolbachia (Hertig) são extensivamente estudados em uma ampla gama de organismos e são conhecidos por serem transmitidos via citoplasma do ovo hospedeiro para seu descendente. Wolbachia pode causar vários tipos de alterações reprodutivas nos artrópodes. Nas espécies de Trichogramma, a reprodução partenogenética induzida por Wolbachia, possibilita as fêmeas dos parasitoides a produção de fêmeas a partir de ovos não fertilizados e estas bactérias estão presentes em pelo menos 9% de todas as espécies de Trichogramma. Estudos filogenéticos têm levado a subdivisão do clado Wolbachia em cinco supergrupos (A, B, C, D and E). Wolbachia em Trichogramma pertence ao supergrupo B. Com o gene wsp foi possível se distinguir quatro grupos de Wolbachia que infectam Trichogramma e adicionar um novo grupo (Ato) devido a inclusão de Wolbachia detectada em Trichogramma atopovirilia (Oatman and Platner, 1983). Primers específicos foram construídos e testados para o grupo "Ato". Setenta e cinco por cento de todas as linhagens de Wolbachia que infectam Trichogramma se enquadraram dentro do grupo "Sib".
Descritores: Proteínas da Membrana Bacteriana Externa/metabolismo
Vespas/microbiologia
Primers do DNA/genética
Alphaproteobacteria/metabolismo
Wolbachia/genética
Genes Bacterianos/genética
-Filogenia
Reprodução
Especificidade da Espécie
Simbiose
Vespas/genética
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME



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