Base de dados : LILACS
Pesquisa : G06.580 [Categoria DeCS]
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Id: lil-736369
Autor: Fernández-Delgado, Milagro; García-Amado, María Alexandra; Contreras, Monica; Incani, Renzo Nino; Chirinos, Humberto; Rojas, Héctor; Suárez, Paula.
Título: Survival, induction and resuscitation of vibrio cholerae from the viable but non-culturable state in the Southern Caribbean sea / Supervivencia, inducción y resucitación de Vibrio cholerae del estado viable no cultivable en el sur del Mar Caribe
Fonte: Rev. Inst. Med. Trop. Säo Paulo;57(1):21-26, Jan-Feb/2015. graf.
Idioma: en.
Resumo: The causative agent of cholera, Vibrio cholerae, can enter into a viable but non-culturable (VBNC) state in response to unfavorable conditions. The aim of this study was to evaluate the in situ survival of V. cholerae in an aquatic environment of the Southern Caribbean Sea, and its induction and resuscitation from the VBNC state. V. cholerae non-O1, non-O139 was inoculated into diffusion chambers placed at the Cuare Wildlife Refuge, Venezuela, and monitored for plate, total and viable cells counts. At 119 days of exposure to the environment, the colony count was < 10 CFU/mL and a portion of the bacterial population entered the VBNC state. Additionally, the viability decreased two orders of magnitude and morphological changes occurred from rod to coccoid cells. Among the aquatic environmental variables, the salinity had negative correlation with the colony counts in the dry season. Resuscitation studies showed significant recovery of cell cultivability with spent media addition (p < 0.05). These results suggest that V. cholerae can persist in the VBNC state in this Caribbean environment and revert to a cultivable form under favorable conditions. The VBNC state might represent a critical step in cholera transmission in susceptible areas.

El agente causal del cólera, Vibrio cholerae, puede entrar a un estado viable no cultivable (VNC) en respuesta a condiciones desfavorables. El objetivo de este estudio fue evaluar la supervivencia in situ de V. cholerae en un ambiente acuático al sur del Mar Caribe y su inducción y resucitación del estado VBNC. V. cholerae no-O1, no-O139 fue inoculado en cámaras de difusión ubicadas en el Refugio de Fauna Cuare, Venezuela, y monitoreado para contaje de colonias, células totales y viables. En 119 días de exposición al ambiente, el contaje de colonias fue < 10 UFC/mL y una fracción de la población bacteriana entró al estado VBNC. Adicionalmente, la viabilidad disminuyó dos órdenes de magnitud y ocurrieron cambios morfológicos de células bacilares a cocoides. Entre las variables del ambiente acuático, la salinidad presentó correlación negativa con el contaje de colonias. Los estudios de resucitación mostraron recuperación significativa de la cultivabilidad celular con adición de sobrenadantes de cultivos en crecimiento activo (p < 0.05). Estos resultados sugieren que V. cholerae puede persistir en estado VBNC en este ambiente de Caribe y revertir a una forma cultivable bajo condiciones favorables. El estado VBNC podría representar un paso crítico en la transmisión del cólera en áreas susceptibles.
Descritores: Viabilidade Microbiana
Vibrio cholerae O1/fisiologia
-Oceano Atlântico
Região do Caribe
Contagem de Colônia Microbiana
Técnicas de Cultura
Microbiologia da Água
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1093565
Autor: García Montero, Majela; González Sosa, Nibaldo; Franco Villavicencio, Humberto; Jimenez Afonso, Gloria Greite.
Título: Evaluación de la viabilidad de Legionella pneumophila en muestras de agua / Evaluation of the viability of Legionella pneumophila in water samples
Fonte: Rev. cuba. med. trop;71(2):e399, mayo.-ago. 2019. graf.
Idioma: es.
Resumo: Introducción: Legionella pneumophila se sitúa entre los principales agentes causales de neumonía adquirida en la comunidad y de origen nosocomial. La inhalación de aerosoles potencialmente contaminados con la bacteria, producto de la colonización de redes y otros sistemas que utilizan agua, representa un peligro para la salud de los individuos expuestos. Objetivo: evaluar la viabilidad de L. pneumophila en muestras de agua almacenadas en diferentes intervalos de tiempo para el diagnóstico por cultivo microbiológico de Legionella spp. Métodos: Se contaminaron artificialmente muestras de agua con dos cepas de L. pneumophila de serogrupos diferentes y la conformación de una mezcla de ellas, para un total de 15 muestras. Los frascos contaminados fueron procesados a las 24 h, 72 h, 7 días, 14 días y 21 días. Se realizó cultivo microbiológico según ISO 11731: 2004 y PNO 03-013: 2015. Resultados: Se demostró viabilidad de la bacteria en muestras almacenadas hasta 21 días. El método de concentración por filtración resultó tener los mayores recobrados del microorganismo. Conclusiones: El tiempo de almacenamiento de las muestras afecta la viabilidad de L. pneumophila. Sienta las bases para estudios posteriores de robustez del diagnóstico de L. pneumophila como parte del servicio que presta el Centro de Investigaciones Científicas de la Defensa Civil en los programas de prevención y control Legionella spp. en instalaciones de interés turístico e industrial(AU)

Introduction: Legionella pneumophila is one of the main causative agents of community- and hospital-acquired pneumonia. Inhalation of sprays potentially contaminated with the bacterium, due to the colonization of networks and other systems using water, is a hazard to the health of exposed individuals. Objective: Evaluate the viability of L. pneumophila in samples of water stored at various time intervals for the microbiological culture diagnosis of Legionella spp. Methods: Water samples were artificially contaminated with two strains of L. pneumophila from different serogroups and a mixture of them, for a total of 15 samples. The contaminated vessels were processed at 24 h, 72 h, 7 d, 14 d and 21 d. Microbiological culture was performed in compliance with ISO 11731: 2004 and PNO 03-013: 2015. Results: The bacterium was found to be viable in samples stored up to 21 days. The filtration concentration method obtained the greatest amount of the microorganism. Conclusions: Storage time of the samples affects the viability of L. pneumophila. The study lays the foundations for further research about the validity of L. pneumophila diagnosis as part of the service offered by the Civil Defense Scientific Research Center in Legionella spp. prevention and control programs for tourist and industrial facilities(AU)
Descritores: Doença dos Legionários/imunologia
Amostras de Água
Viabilidade Microbiana/imunologia
-Pneumonia/microbiologia
Comunicação
Responsável: CU1.1 - Biblioteca Médica Nacional


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Bachion, Maria Márcia
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Id: lil-600079
Autor: Quege, Geovana Eloisa; Bachion, Maria Márcia; Lino Junior, Ruy de Souza; Lima, Ana Beatriz Mori; Ferreira, Priscila Santos; Santos, Queiliene Rosa dos; Pimenta, Fabiana Cristina.
Título: Comparação da atividade de ácidos graxos essenciais e biomembrana na microbiota de feridas crônicas infectadas / Comparison of the activity of fatty essential acids and biomembrane in the microbiota of infected chronic wounds
Fonte: Rev. eletrônica enferm;10(4), 2008.
Idioma: pt.
Resumo: Em pessoas curadas de hanseníase, as úlceras em membros inferiores representam um desafio aos profissionais, por serem crônicas, infectadas e recorrentes. Esta pesquisa teve como objetivo comparar a ação de uma biomembrana de látex (Biocure®) e de um produto à base de AGE (Dersani®) na microbiota de feridas crônicas infectadas, em pessoas que receberam alta do tratamento para hanseníase. Trata-se de pesquisa realizada no Hospital de Dermatologia Sanitária, de Goiânia (GO), no período de fevereiro a outubro de 2007, na qual participaram 8 pessoas curadas de hanseníase, com 19 feridas infectadas, que foram, alocadas aleatoriamente em grupo A (tratado com Dersani®) e grupo B (tratado com Biocure®). Foram identificados Staphylococcus aureus (50%), Pseudomonas aeruginosa (35,7%), Proteus vulgaris (8,2%), Enterobacter aerogenes (3,3%) e Escherichia coli (2,7%). Os resultados obtidos in vivo sugerem que o Dersani® tenha efeito antimicrobiano positivo sobre Enterobacter aerogenes e o Biocure® sobre Pseudomonas aeruginosa. Os resultados in vitro mostraram ausência de atividade de ambos os produtos sobre os microrganismos isolados das lesões.

For patients cured of leprosy, low-limb ulcers represent a challenge to healthcare workers, for being chronic, infected, and recurrent. This study aimed to compare the latex biomembrane (Biocure TM) and an EFA-based product (Dersani TM) action on the microbiota of infected chronic wounds, in patients discharged from the treatment of leprosy. This is a research carried out at Hospital de Dermatologia Sanitária, in Goiânia-GO, from February to October 2007, in which 8 individuals cured of leprosy, having 19 infected wounds, were randomly allocated in group A (Dersani TM) and group B (Biocure TM). Staphylococcus aureus (50%), Pseudomonas aeruginosa (35.7%), Proteus vulgaris (8.2%), Enterobacter aerogenes (3.3%), and Escherichia coli (2.7%) were isolated. The results obtained in vivo suggest that Dersani TM has a positive antimicrobial effect against Enterobacter aerogenes and Biocure TM against Pseudomonas aeruginosa, despite the in vitro results having shown absence of antimicrobial activity for both products against microorganisms standard and isolated from the wounds.
Descritores: Cicatrização
Infecção dos Ferimentos
Viabilidade Microbiana
-Hanseníase/terapia
Úlcera da Perna/terapia
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Responsável: BR584.1 - Biblioteca Central BSCAN


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Id: biblio-1120029
Autor: Teixeira, Lara Azevedo; Carvalho, Wellington Roberto Gomes de.
Título: SARS-CoV-2 em superfícies: persistência e medidas preventivas: uma revisão sistemática / SARS-CoV-2 in Surfaces: persistence and prevention measures: a systematic review / SARS-CoV-2 en Superficies: persistencia y medidas preventivas: una revisión sistemática
Fonte: J. Health NPEPS;5(2), set. 2020.
Idioma: pt.
Resumo: Objetivo: verificar a persistência do SARS-CoV-2 nas diferentes superfícies e medidas preventivas contra a transmissão do vírus. Método: revisão sistemática norteada pelo método PRISMA. Foram utilizadas as bases de buscas PubMed e LILACS de janeiro a junho de 2020, com os descritores: "2019-nCOV" OR "SARS-CoV-2" OR "COVID-19" AND "transmission" OR "transmission route" AND "viability" AND "surface" OR "inanimate surface" AND "prevention". As informações extraídas foram autor/ano, país, tipo de publicação, nome da revista, idioma, país da publicação e base de dados. Resultados: foram identificadas 178 publicações, com exclusão de 164 artigos, nove por idioma, 12 por outras doenças e/ou patógenos e 143 pelo título e/ou resumo. Foram incluídos 14 artigos qualitativos, oito artigos de revisões narrativas, uma comunicação breve, dois artigos originais e um editorial. Treze artigos foram publicados em inglês e um em português. Conclusão: coronavírus humanos (HCoV 229E) podem se manter em diferentes superfícies durante duas horas até nove dias. Baixas temperaturas e reduzida umidade relativa do ar favorecem a sobrevivência do SARS-CoV-2, sendo mais estável em plásticos e aço inoxidável do que em cobre e papelão. A recomendação é higienização de superfícies e mãos com água, sabão ou higienizadores à base de álcool.(AU)

Objective: to verify the persistence of SARS-CoV-2 on different types of surfaces and the preventive measures against the transmission of the virus. Method: a systematic review was carried out, using the PRISMA method. The PubMed and LILACS databases from January to June 2020 were used, with the following descriptors: "2019-nCOV" OR "SARS-CoV-2" OR "COVID-19" AND "transmission" OR "transmission route" AND "viability" AND "surface" OR "inanimate surface" AND "prevention". Information extracted was author/year, country, type of publication, journal name, language, country of publication and database. Results: 178 publications were identified. 164 articles were excluded, nine by language, 12 by other diseases and/or pathogens and 143 by title and/or abstract. 14 qualitative articles were included, eight articles of narrative reviews, one short communication, two original articles and one editorial. Thirteen articles were published in English and one in Portuguese. Conclusion: human coronaviruses (HCoV 229E) can persist on different surfaces for two hours up to nine days. Low temperatures and low relative humidity of the air favor the survival of SARS-CoV-2, which is more stable on plastics and on stainless steel than on copper and cardboard. The recommendation is frequent surface and hand hygiene with water, soap or alcohol-based rubs.(AU)

Objetivo: verificar la persistencia del SARS-CoV-2 en diferentes superficies y las medidas preventivas contra la transmisión del virus. Método: se realizó una revisión sistemática, utilizando el método PRISMA. Se utilizaron las bases de datos de búsqueda de PubMed y LILACS de enero a junio de 2020, con los descriptores: "2019-nCOV" O "SARS-CoV-2" O "COVID-19" Y "transmisión" O "ruta de transmisión" Y "viabilidad" Y "superficie" O "superficie inanimada" Y "prevención". Las informaciones extraídas fueron autor / año, país, tipo de publicación, nombre de la revista, idioma, país de publicación y base de datos. Resultados: se identificaron 178 publicaciones. Se excluyeron 164 artículos, nueve por idioma, 12 por otras enfermedades y/o patógenos y 143 por título y/o resumen, incluidos 14 artículos cualitativos, ocho artículos de revisiones narrativas, una comunicación breve, dos artículos originales y uno editorial. Se publicaron trece artículos en inglés y uno en portugués. Conclusión: los coronavirus humanos (HCoV 229E) pueden matenerse en diferentes superficies durante dos horas hasta nueve días. Las bajas temperaturas y la reducida humedad relativa del aire favorecen la supervivencia del SARS-CoV-2, siendo más estable en plásticos y acero inoxidable que en cobre y carton. La recomendación es limpiar superficies y manos con agua, jabón o limpiadores a base de alcohol.(AU)
Descritores: Infecções por Coronavirus/prevenção & controle
Prevenção de Doenças
Viabilidade Microbiana
Betacoronavirus/isolamento & purificação
-Higiene das Mãos
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-1087459
Autor: Olivares, Araceli; Soto, Carmen; Caballero, Eduardo; Altamirano, Claudia.
Título: Survival of microencapsulated Lactobacillus casei (prepared by vibration technology) in fruit juice during cold storage
Fonte: Electron. j. biotechnol;42:42-48, Nov. 2019. graf, tab, ilus.
Idioma: en.
Projeto: FONDECYT; . Centro Regional de Estudios en Alimentos Saludables (CREAS), Regional Program, Chile, GORE-CONICYT.
Resumo: Background: Foods including probiotics are considered "functional foods." As an alternative to dairy products, we investigated the behavior of Lactobacillus casei when exposed to low-pH fruit juice. Juices of fruits such as pineapple, raspberry, and orange were assessed. Free and microencapsulated forms of L. casei were compared, and the viability of the probiotic was evaluated under storage at 4°C for 28 d. Microbiological analyses were carried out to ensure a safe and healthy product for consumers who look for foods with probiotics from sources other than dairy. Results: Low pH affected L. casei survival during storage depending on the type of fruit juice. In the case of pineapple juice, some microcapsules were broken, but microcapsules recovered at the end of the storage period had 100% viability (2.3 × 107 CFU/g spheres). In the case of orange juice, more than 91% viability (5.5 × 106 CFU/g spheres) was found. In raspberry juice, viability decreased rapidly, disappearing at the end of the storage period, which was caused by the absorption of high concentrations of anthocyanin inside microcapsules more than low pH. Conclusion: Low pH affected the survival of L. casei under refrigeration; even when they were microencapsulated, acidic conditions impacted their viability. Although pH affects viability, its value is very sensitive and will depend on the type of fruit juice and its composition. Some fruit juices contain compounds used as substrates for Lactobacillus and other compounds with antimicrobial effects.
Descritores: Viabilidade Microbiana
Sucos de Frutas e Vegetais
Lactobacillus casei/crescimento & desenvolvimento
-Vibração
Temperatura Baixa
Probióticos
Alginatos/química
Armazenamento de Alimentos
Pasteurização
Concentração de Íons de Hidrogênio
Antocianinas
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1087706
Autor: Zhang, Zheng; Yu, Yong-xiang; Wang, Yin-geng; Wei, Xin-xian; Liao, Mei-jie; Rong, Xiao-jun; Chen, Jing.
Título: Development of a new protocol for freeze-drying preservation of Pseudoalteromonas nigrifaciens and its protective effect on other marine bacteria
Fonte: Electron. j. biotechnol;44:1-5, Mar. 2020. graf, tab.
Idioma: en.
Projeto: The Key Projects of Science and Technology Innovation of Shandong Province; . Special Founds of Innovation-driven Development of Guangxi; . Central Public-interest Scientific Institution Basal Research Fund, Yellow Sea Fisheries Research Institutes, CAFS.
Resumo: Background: Freeze-drying is known as one of the best methods to preserve bacterial strains. Protectant is the key factor affecting the survival rate of freeze-dried strains. In addition, salinity, bacterial suspension concentration, drying time, and other factors can also affect the survival rate of strains to varying degrees. At present, there are relatively few studies on freeze-drying preservation of marine bacteria. In the present study, we performed the freeze-drying protectant screening and optimized the preservation conditions for Pseudoalteromonas nigrifaciens, which is widely distributed in marine environment. The protective effects of the screened protectants were verified by 18 other marine bacterial strains. Results: The results indicated that the combination of 5.0% (w/v) lactose, 5.0% (w/v) mannitol, 5.0% (w/v) trehalose, 10.0% (w/v) skim milk powder, 0.5% (w/v) ascorbic acid and 0.5% (w/v) gelatin was the best choice for the preservation of P. nigrifaciens. The suggested salinity and concentration of initial cell suspension were 10 g/L NaCl and 1.0 × 109 CFU/mL, respectively. Furthermore, stationary-phase cells were the best choice for the freeze-drying process. The highest survival rate of P. nigrifaciens reached 52.8% when using 5­10% (w/v) skim milk as rehydration medium. Moreover, the other 18 marine strains belonging to Pseudoalteromonas, Vibrio, Photobacterium, Planomicrobium, Edwardsiella, Enterococcus, Bacillus, and Saccharomyces were freezedried under the abovementioned conditions. Their survival rates were 2.3­95.1%. Conclusion: Collectively, our results supported that the protectant mixture and parameters were beneficial for lyophilization of marine bacteria
Descritores: Preservação Biológica/métodos
Pseudoalteromonas/fisiologia
Liofilização/métodos
-Trealose/química
Sobrevivência Celular
Fenômenos Fisiológicos Bacterianos
Dissacarídeos/química
Viabilidade Microbiana
Salinidade
Lactose/química
Manitol/química
Responsável: CL1.1 - Biblioteca Central


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Beletti, Marcelo Emílio
Rossi, Daise Aparecida
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Id: lil-709481
Autor: Fonseca, Belchiolina Beatriz; Beletti, Marcelo Emílio; Melo, Roberta Torres de; Mendonça, Eliane Pereira; Coelho, Letícia Ríspoli; Nalevaiko, Priscila Christen; Rossi, Daise Aparecida.
Título: Campylobacter jejuni in commercial eggs
Fonte: Braz. j. microbiol;45(1):76-79, 2014. ilus, tab.
Idioma: en.
Resumo: This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.
Descritores: Campylobacter jejuni/isolamento & purificação
Campylobacter jejuni/fisiologia
Ovos/microbiologia
Viabilidade Microbiana
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Id: biblio-951802
Autor: Djordjevic, Jasna; Boskovic, Marija; Starcevic, Marija; Ivanovic, Jelena; Karabasil, Nedjeljko; Dimitrijevic, Mirjana; Brankovic Lazic, Ivana; Baltica, Milan Z.
Título: Survival of Salmonella spp. in minced meat packaged under vacuum and modified atmosphere
Fonte: Braz. j. microbiol;49(3):607-613, July-Sept. 2018. tab.
Idioma: en.
Resumo: Abstract The effect of different modified atmosphere packaging regimes on the behavior of Salmonella spp. on minced meat was studied. Minced meat was experimentally contaminated with a Salmonella spp. cocktail (S. Enteritidis, S. Typhimurium, S. Infantis and S. Arizonae), packaged under vacuum or modified atmosphere with initial headspaces containing 20%O2/50%CO2/30%N2 and 20%O2/30%CO2/50%N2) and stored at 3 ± 1 °C for 12 days. Samples were analyzed for Salmonella spp., viable and lactic acid bacteria count every third day. Salmonella spp. counts decreased during storage in all packaging types, with reductions of about 1.5 log CFU/g. A significant difference (p < 0.01) was noted between Salmonella spp. counts in meat packaged in vacuum and modified atmospheres, although there was no significant difference in Salmonella spp. count between meat packaged in 50%CO2, and meat packaged in 30%CO2. At the end of the study, there were significant differences (p < 0.01; p < 0.05) in total viable and lactic acid bacterial counts between meat packaged in vacuum and modified atmosphere, and the lowest counts were noted in meat packaged in modified atmosphere with 50%CO2.
Descritores: Salmonella/crescimento & desenvolvimento
Embalagem de Alimentos/métodos
Viabilidade Microbiana
Carne/microbiologia
-Salmonella/isolamento & purificação
Salmonella/genética
Suínos
Vácuo
Contagem de Colônia Microbiana
Embalagem de Alimentos/instrumentação
Carne/análise
Limites: Animais
Bovinos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-828188
Autor: Farooq, Muzammil; Freed, Shoaib.
Título: Infectivity of housefly, Musca domestica (Diptera: Muscidae) to different entomopathogenic fungi
Fonte: Braz. j. microbiol;47(4):807-816, Oct.-Dec. 2016. tab.
Idioma: en.
Resumo: Abstract The housefly Musca domestica is a worldwide insect pest that acts as a vector for many pathogenic diseases in both people and animals. The present study was conducted to evaluate the virulence of different local isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea on M. domestica using two bioassay techniques: (1) adult immersion and (2) a bait method applied to both larvae and adults. The results showed evidence of a broad range of responses by both stages (larvae and adults) to the tested isolates of B. bassiana, M. anisopliae and I. fumosorosea. These responses were concentration-dependent, with mortality percentages ranging from 53.00% to 96.00%. Because it resulted in lower LC50 values and a shorter lethal time, B. bassiana (Bb-01) proved to be the most virulent isolate against both housefly larvae and adults. Sublethal doses of the tested isolates were also assessed to evaluate their effect on M. domestica fecundity and longevity. The fungal infections reduced housefly survival regardless of their sex and also decreased egg production in females.
Descritores: Fungos/fisiologia
Moscas Domésticas/microbiologia
-Paquistão
Viabilidade Microbiana
Fungos/isolamento & purificação
Larva/microbiologia
Limites: Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Id: lil-788979
Autor: Oladunjoye, Adebola O; Singh, Suren; Ijabadeniyi, Oluwatosin A.
Título: Inactivation of Listeria monocytogenes ATCC 7644 on fresh-cut tomato using nisin in combinations with organic salts
Fonte: Braz. j. microbiol;47(3):757-763, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT The inhibition of Listeria monocytogenes ATCC 7644 on fresh-cut tomato was investigated using nisin alone, and in combinations with organic salts. Nisin at a concentration of 5000 UI/mL was introduced alone or in combination with an organic salt (sodium citrate or sodium acetate each at 3 and 5 g/100 mL each) on fresh-cut tomato previously inoculated with 108 CFU/mL of L. monocytogenes ATCC 7644. Chlorine at 200 ppm was used as a control. The inoculated samples were incubated at different temperatures (4, 10 and 25 °C) and examined at 0, 24, 48 and 72 h. The effects of the antimicrobial treatments on quality parameters of tomato (pH, soluble solids, titratable acidity and vitamin C) were also evaluated, and colour parameters were observed at the lowest storage temperature for 10 days. Both nisin and the organic salts inhibited growth of L. monocytogenes, but the combinations of two compounds were more effective. The nisin-sodium citrate (5%) combination was significantly (p ≤ 0.05) effective, while chlorine was least effective against L. monocytogenes. The quality parameters were substantially retained, especially at 4 °C, suggesting good shelf stability at a low temperature. These results substantiate the use of the cheap and eco-friendly approach to reducing this pathogen of health concern in common fresh produce.
Descritores: Sais/farmacologia
Lycopersicon esculentum/microbiologia
Listeria monocytogenes/efeitos dos fármacos
Nisina/farmacologia
-Contagem de Colônia Microbiana
Viabilidade Microbiana/efeitos dos fármacos
Microbiologia de Alimentos
Conservação de Alimentos/métodos
Conservantes de Alimentos
Listeria monocytogenes/isolamento & purificação
Antibacterianos/farmacologia
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde