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Id: biblio-844458
Autor: Castrillón-Betancur, Juan Camilo; Urcuqui-Inchima, Silvio.
Título: Avances en la investigación del virus dengue en Colombia: papel de los microARNs celulares en la respuesta anti-dengue virus / Advances in dengue virus research in Colombia: the role of cellular microRNAs as an anti-dengue virus response
Fonte: Rev. chil. infectol;34(2):143-148, abr. 2017. ilus.
Idioma: es.
Resumo: Dengue is one of the most important mosquito-borne diseases, and its incidence has increased at an alarming rate in recent years, becoming a real public health problem. Currently, there is no vaccine or medication or proper treatment for dengue control. Considering this situation, it is necessary to prioritize the search for new alternatives and strategies for dengue prevention and control, in order to reduce not only the economic burden of endemic countries, but also to improve the quality of life of patients. In this regard, a brief reflection on some aspects related to the search for new alternatives in Colombia is presented. This is focused on the use of microRNAs, which could be a new strategy with great therapeutic potential.

El dengue es una de las enfermedades más importantes transmitidas por mosquitos y su incidencia ha aumentado a un ritmo alarmante en los últimos años, al punto que se ha convertido en un verdadero problema de salud pública. Actualmente no existe ni vacuna, ni un medicamento o tratamiento adecuado para el control del dengue. Con dichos antecedentes, es necesario priorizar en la búsqueda de nuevas alternativas o estrategias de control y prevención del dengue con miras a disminuir no sólo la carga económica de los países endémicos, sino también a mejorar la calidad de vida de los pacientes. En este sentido, se presenta una breve reflexión sobre algunos aspectos relacionados con la búsqueda de nuevas alternativas en Colombia, enfocadas en el uso de los microARNs, que podrían constituir una nueva estrategia con un gran potencial terapéutico, dado que tendrían el potencial de contrarrestar algunas infecciones virales crónicas.
Descritores: Replicação Viral/genética
Vírus da Dengue/genética
MicroRNAs/metabolismo
-Biossíntese de Proteínas
Colômbia
MicroRNAs/genética
Pesquisa Biomédica
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-899708
Autor: Martínez, Jahnnyer; Hernández, Juan C; Urcuqui-Inchima, Silvio.
Título: Papel de las células dendríticas en la infección por el virus dengue: blancos de replicación y respuesta inmune / Role of dendritic cells in infection by dengue virus: targets for replication and immune response
Fonte: Rev. chil. infectol;34(3):249-256, jun. 2017. ilus, tab.
Idioma: es.
Projeto: Colciencias; . Universidad de Antioquia.
Resumo: Dengue fever, caused by dengue virus (DENV) infection, is one of the most important diseases in the world, not only due to the high morbidity/mortality rates it causes, but also because of its great economic and social impact in tropical/subtropical countries. DENV infection has a wide range of clinical manifestations ranging from asymptomatic infection or infection with mild symptoms to severe dengue that can lead to death. At present, no etiological treatment or effective globally distributed vaccine against the four DENV serotypes exists. Despite great efforts made to understand the mechanism associated with DENV disease pathogenesis the causes leading to severe dengue presentation have not been clarified. Some hypotheses seek to give a biological and physiological explanation to the clinical manifestations that appear during the infection. Based on the evidence that after contact with dendritic cells DENV alters the functionality of these cells, this review aims to describe the most relevant findings regarding the importance of dendritic cells in the context of DENV infection and progression of the illness.

El dengue, causada por el virus dengue (DENV), es una de las enfermedades más importantes no sólo por los altos índices de morbilidad/mortalidad, sino también por su gran impacto económico y social en los países de las regiones tropicales/subtropicales. La infección por el DENV cursa por un variado rango de manifestaciones clínicas que van desde una infección asintomática o con síntomas leves, hasta el dengue grave que puede ser fatal. En la actualidad, no se dispone de un tratamiento etiológico y tampoco de una vacuna eficaz mundialmente distribuida, contra los 4 serotipos del DENV. A pesar de los grandes esfuerzos orientados a entender el mecanismo asociado con la patogénesis de la enfermedad, aún no se ha logrado esclarecer de forma definitiva las causas que conllevan a las formas graves de enfermedad. Algunas hipótesis buscan dar una explicación biológica y fisiológica a las manifestaciones clínicas que se presentan durante la infección. Dado que una de ellas sugiere que luego del contacto con las células dendríticas el DENV altera su funcionalidad, la presente revisión tiene como objetivo describir los hallazgos más relevantes referentes a la importancia de dichas células en el marco de la infección por el DENV y progresión de la enfermedad.
Descritores: Replicação Viral/imunologia
Células Dendríticas/imunologia
Dengue/imunologia
Vírus da Dengue/imunologia
-Progressão da Doença
Imunidade Celular
Imunidade Inata
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-952838
Autor: Prati, Bruna; Marangoni, Bruna; Boccardo, Enrique.
Título: Human papillomavirus and genome instability: from productive infection to cancer
Fonte: Clinics;73(supl.1):e539s, 2018. graf.
Idioma: en.
Projeto: FAPESP; . CNPq; . CNPq; . CAPES.
Resumo: Infection with high oncogenic risk human papillomavirus types is the etiological factor of cervical cancer and a major cause of other epithelial malignancies, including vulvar, vaginal, anal, penile and head and neck carcinomas. These agents affect epithelial homeostasis through the expression of specific proteins that deregulate important cellular signaling pathways to achieve efficient viral replication. Among the major targets of viral proteins are components of the DNA damage detection and repair machinery. The activation of many of these cellular factors is critical to process viral genome replication intermediates and, consequently, to sustain faithful viral progeny production. In addition to the important role of cellular DNA repair machinery in the infective human papillomavirus cycle, alterations in the expression and activity of many of its components are observed in human papillomavirus-related tumors. Several studies from different laboratories have reported the impact of the expression of human papillomavirus oncogenes, mainly E6 and E7, on proteins in almost all the main cellular DNA repair mechanisms. This has direct consequences on cellular transformation since it causes the accumulation of point mutations, insertions and deletions of short nucleotide stretches, as well as numerical and structural chromosomal alterations characteristic of tumor cells. On the other hand, it is clear that human papillomavirus-transformed cells depend on the preservation of a basal cellular DNA repair activity level to maintain tumor cell viability. In this review, we summarize the data concerning the effect of human papillomavirus infection on DNA repair mechanisms. In addition, we discuss the potential of exploiting human papillomavirus-transformed cell dependency on DNA repair pathways as effective antitumoral therapies.
Descritores: Papillomaviridae/genética
Infecções por Papillomavirus/virologia
Reparo do DNA
Neoplasias/virologia
-Papillomaviridae/fisiologia
Replicação Viral
Linhagem Celular Transformada/virologia
Sobrevivência Celular/genética
Instabilidade Genômica/genética
Neoplasias/terapia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-1041442
Autor: Queiroz, Sabrina Ribeiro de Almeida; Silva Júnior, José Valter Joaquim; Silva, Andréa Nazaré Monteiro Rangel da; Carvalho, Amanda Gomes de Oliveira; Santos, Jefferson José da Silva; Gil, Laura Helena Vega Gonzales.
Título: Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Fonte: Rev. Soc. Bras. Med. Trop;51(1):66-70, Jan.-Feb. 2018. graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. METHODS: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. RESULTS: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. CONCLUSIONS: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
Descritores: Replicação Viral/imunologia
Vírus da Febre Amarela/imunologia
Montagem de Vírus/imunologia
Vacinas de Partículas Semelhantes a Vírus/imunologia
-Replicação Viral/genética
Vírus da Febre Amarela/genética
Montagem de Vírus/genética
Técnica Indireta de Fluorescência para Anticorpo
Proteínas de Fluorescência Verde
Células HEK293
Vacinas de Partículas Semelhantes a Vírus/genética
Citometria de Fluxo
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-957459
Autor: Caldas, Lucio Ayres; Ferreira, Davis Fernandes; Freitas, Tânia Rosaria Pereira.
Título: Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model
Fonte: Rev. Soc. Bras. Med. Trop;51(5):584-590, Sept.-Oct. 2018. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: The Mayaro virus (MAYV), which is an arbovirus closely related to the Chikungunya virus, causes a dengue-like acute illness that is endemic to Central and South America. We investigated the anti-MAYV activity of prostaglandin A1 (PGA1), a hormone which exhibits antiviral activity against both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) viruses. Further, we examined the effects of inducting the stress protein HSP70 following PGA1 treatment. METHODS: Hep-2 cells infected with MAYV were treated with PGA1 (0.1-6μg/ml) 12h before infection and for different periods post-infection. Inhibition of viral replication inhibition was analyzed via viral titer determination, whereas the effect of PGA1 on viral morphogenesis was examined via transmission electron microscopy (TEM). Autoradiography (with 35S methionine labeling) and western blotting were used to assess the effect of PGA1 treatment on viral and cellular protein synthesis, and on HSP70 induction, respectively. RESULTS: PGA1 strongly reduced viral replication in Hep-2 cells, particularly when added during the early stages of viral replication. Although PGA1 treatment inhibited viral replication by 95% at 24 hours post-infection (hpi), viral structural protein synthesis was inhibited only by 15%. TEM analysis suggested that PGA1 inhibited replication before viral morphogenesis. Western blot and densitometry analyses showed that PGA1 treatment increased HSP70 protein levels, although this was not detectable via autoradiography. CONCLUSIONS: PGA1 inhibits MAYV replication in Hep-2 cells at early stages of viral replication, prior to production of viral structural proteins, possibly via HSP70 induction.
Descritores: Prostaglandinas A/farmacologia
Replicação Viral/efeitos dos fármacos
Alphavirus/efeitos dos fármacos
Proteínas de Choque Térmico HSP70/farmacologia
Células Epiteliais/virologia
-Antivirais/farmacologia
Linhagem Celular
Western Blotting
Alphavirus/ultraestrutura
Microscopia Eletrônica de Transmissão
Eletroforese em Gel de Poliacrilamida
Células Epiteliais/ultraestrutura
Limites: Humanos
Animais
Bovinos
Responsável: BR1.1 - BIREME


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Id: biblio-1003127
Autor: Guerrero, Nidya Alexandra Segura; García, Felio Jesús Bello.
Título: Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180511, 2019. graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Insect cell cultures play an essential role in understanding arboviral replication. However, the replicative efficiency of some of these viruses such as dengue (DENV), yellow fever (YFV), and chikungunya (CHIKV) in a new cellular substrate (Lulo) and in the other two recognized cell lines has not been comparatively assessed. METHODS: Vero, C6/36, and Lulo cell lines were infected with DENV, YFV, and CHIKV. The viral progeny was quantified through plaque assays and quantitative reverse transcription-polymerase chain reaction, while for DENV2, the findings were confirmed by immunofluorescence antibody assay. RESULTS: The higher DENV2 titer (from multiplicity of infection 0.001) was obtained on day four post-infection in C6/36 and on day six in Vero cells, while the Lulo cell line was almost impossible to infect under the same conditions. However, C6/36 showed the highest values of viral RNA production compared to Vero cells, while the quantification of the viral RNA in Lulo cells showed high levels of viral genomes, which had no correlation to the infectious viral particles. CONCLUSIONS: C6/36 was the most efficient cell line in the alpha and flavivirus production, followed by Vero cells. Thus, Lulo cells may be a useful substrate to study the mechanisms by which cells evade viral replication.
Descritores: Replicação Viral/fisiologia
Vírus da Febre Amarela/fisiologia
Vírus Chikungunya/fisiologia
Vírus da Dengue/fisiologia
Insetos/virologia
-Fatores de Tempo
Células Vero
Chlorocebus aethiops
Cricetinae
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Animais
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-1041501
Autor: Dessordi, Renata; Santana, Rodrigo de Carvalho; Navarro, Anderson Marliere.
Título: Influence of antiretroviral therapy on bone metabolism of patients with chronic hepatitis B: a review
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180441, 2019.
Idioma: en.
Projeto: Sao Paulo Research Foundation.
Resumo: Abstract Hepatitis B is a major public health problem worldwide and associated with significant mortality. To prevent or delay the deleterious effects of chronic infection by the hepatitis B virus, patients should be carefully followed, and antiviral therapy indicated according to specific recommendations. Currently, available drugs inhibit viral replication and slow or stop the progression of inflammation and fibrosis of the liver. However, the drugs for oral use in the treatment of hepatitis B, jointly referred to as nucleoside/nucleotide analogs, are indicated for prolonged use and have potential side effects. The reduction in bone mineral density was associated with the use of tenofovir, already evaluated in patients infected with HIV because the drug is also part of the therapeutic arsenal for this viral infection. There are few studies on the effects of tenofovir in patients with mono hepatitis B. Therefore, this literature review proposes to examine how hepatitis B acts in the body and the mechanisms by which antiretroviral drugs (especially tenofovir) can affect bone metabolism.
Descritores: Densidade Óssea/efeitos dos fármacos
Remodelação Óssea/efeitos dos fármacos
Hepatite B Crônica/tratamento farmacológico
Antirretrovirais/efeitos adversos
-Replicação Viral/efeitos dos fármacos
Antirretrovirais/administração & dosagem
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: lil-742540
Autor: Retamal-Díaz, Angello R; Suazo, Paula A; Garrido, Ignacio; Kalergis, Alexis M; González, Pablo A.
Título: Evasión de la respuesta inmune por virus herpes simplex / Immune evasion by herpes simplex viruses
Fonte: Rev. chil. infectol;32(1):58-70, feb. 2015. ilus.
Idioma: es.
Projeto: FONDECYT; . FONDECYT; . FONDECYT; . Instituto Milenio en Inmunología e Inmunoterapia.
Resumo: Herpes simplex viruses and humans have co-existed for tens of thousands of years. This long relationship has translated into the evolution and selection of viral determinants to evade the host immune response and reciprocally the evolution and selection of host immune components for limiting virus infection and damage. Currently there are no vaccines available to avoid infection with these viruses or therapies to cure them. Herpes simplex viruses are neurotropic and reside latently in neurons at the trigeminal and dorsal root ganglia, occasionally reactivating. Most viral recurrences are subclinical and thus, unnoticed. Here, we discuss the initial steps of infection by herpes simplex viruses and the molecular mechanisms they have developed to evade innate and adaptive immunity. A better understanding of the molecular mechanisms evolved by these viruses to evade host immunity should help us envision novel vaccine strategies and therapies that limit infection and dissemination.

Los virus herpes simplex y humanos co-existen desde decenas de miles de años. Esta prolongada relación se ha traducido en la evolución y selección de determinantes virales para evadir la respuesta inmune y recíprocamente la evolución y selección de componentes inmunes del hospedero para limitar la infección viral y el daño que producen. Actualmente no existen vacunas para evitar la infección de estos virus o terapias que la curen. Los virus herpes simplex son neurotrópicos y permanecen latentes en neuronas de ganglios trigémino y dorsales, reactivándose esporádicamente. La mayoría de las recurrencias por virus herpes simplex son sub-clínicas y por tanto pasan inadvertidas. Aquí discutimos los pasos iniciales de la infección porvirus herpes simplex y los mecanismos moleculares que estos virus han desarrollado para evadir la respuesta inmune innata y adaptativa. Una mejor comprensión de los mecanismos moleculares evolucionados por estos virus para evadir la respuesta inmune del hospedero deberían ayudarnos visualizar nuevas estrategias para desarrollar vacunas y terapias que limiten su infección y diseminación.
Descritores: Imunidade Adaptativa/imunologia
Herpes Simples/imunologia
Evasão da Resposta Imune
Simplexvirus/patogenicidade
-Apoptose/fisiologia
Interferon Tipo I/imunologia
Simplexvirus/fisiologia
Latência Viral/fisiologia
Replicação Viral/fisiologia
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1011412
Autor: Li, Wenting; Yu, Xiaolan; Zhu, Chuanlong; Wang, Zheng; Zhao, Zonghao; Li, Yi; Zhang, Yonghong.
Título: Notum attenuates HBV-related liver fibrosis through inhibiting Wnt 5a mediated non-canonical pathways
Fonte: Biol. Res;52:10, 2019. tab, graf.
Idioma: en.
Projeto: WBE Liver Fibrosis Foundation; . International Cooperative Project of Anhui Province; . Fundamental Research Funds for the Central Universities.
Resumo: BACKGROUND: Non-canonical Wnt pathways play important roles in liver fibrosis. Notum is a newly discovered inhibitor to Wnt proteins. This study was to investigate anti-fibrotic effects of Notum. METHODS: 53 patients with hepatitis B virus (HBV) infection as well as a cell co-culture system of LX-2 and Hep AD38 cells were engaged in this study. Clinical, biological and virological data of each patient were analyzed. Cell viability was detected at different time points. mRNA and protein levels of NFATc1 (Nuclear factor of activated T-cells), Jnk, α-SMA, Col1A1 and TIMP-1 were detected both in LX-2 and liver tissue. Protein levels of NFATc1 and Jnk in liver tissue and their correlations with fibrosis score were analyzed. RESULTS: Hepatitis B virus replication up-regulated Wnt5a induced NFATc1 and Jnk activity in Hep AD38. Notum suppressed NFATc1, Jnk and fibrosis genes expression, reduced cell viability in co-cultured LX-2 cells induced by HBV. Interestingly, Patients with HBV DNA > 5log copies/ml had higher mRNA levels of NFATc1 and fibrosis genes than patients with HBV DNA < 5log copies/ml. Most importantly, protein expressions of NFATc1 and pJnk have positive correlations with liver fibrosis scores in HBV-infected patients. CONCLUSIONS: Our data showed that Notum inhibited HBV-induced liver fibrosis through down-regulating Wnt 5a mediated non-canonical pathways. This study shed light on anti-fibrotic treatment.
Descritores: Esterases/administração & dosagem
Proteína Wnt-5a/antagonistas & inibidores
Hepatite B/complicações
Cirrose Hepática/prevenção & controle
-Replicação Viral
Transfecção
Sobrevivência Celular
Vírus da Hepatite B/fisiologia
Actinas/metabolismo
Inibidor Tecidual de Metaloproteinase-1/metabolismo
Colágeno Tipo I/metabolismo
MAP Quinase Quinase 4/metabolismo
Fatores de Transcrição NFATC/análise
Fatores de Transcrição NFATC/metabolismo
Via de Sinalização Wnt
Proteína Wnt-5a/metabolismo
Cirrose Hepática/metabolismo
Cirrose Hepática/virologia
Limites: Humanos
Masculino
Feminino
Adulto
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1148189
Autor: Hsieh, Priscila Garcia; Siqueira, Laurita Cardoso; Zilly, Adriana; Assolini, João Paulo; Silva, Aline Preve da; Cezar-dos-Santos, Fernando.
Título: Investigating the role of circulating microRNAs in human immunodeficiency virus infection: friends or foes
Fonte: Clin. biomed. res;40(2), 2020. ilus.
Idioma: en.
Resumo: Introduction: Human immunodeficiency virus (HIV), which causes Acquired Immunodeficiency Syndrome, still affects millions of people worldwide. Despite recent advances in the understanding of biological mechanisms of viral replication, there are relevant gaps regarding the virus-host relationship. Unraveling these complexities may lead to the development of new therapeutic strategies and the establishment of new biomarkers useful for the diagnosis and prognosis of infection and its comorbidities. Therefore, in this study we discuss the main biological characteristics of microRNAs and the potential use of these nucleic acids in their free circulating form as indicators of risk or protection against HIV infection. Methods: A narrative review of the literature was carried out in the following databases through keyword and/or health descriptor searches: i) Google Scholar; ii) CAPES periodicals portal; iii) United States National Library of Medicine (PubMed) and iv) Elsevier's Science Direct library. The keywords "microRNA; HIV infection; circulating microRNA; biomarkers" were used to search the databases as mentioned above.Results: Circulating microRNAs (ci-miRNA) are closely related to numerous processes in the HIV infection pathophysiology. They are involved in viral latency, increased viremia, hepatic injury, heart dysfunction, pulmonary hypertension, immune response impairment, and participate in Kaposi's sarcoma pathology. Additionally, these molecules may indicate protection in elite controllers, reduce viral replication and load, and be useful markers of the infection's eclipse phase. Conclusion: Ci-miRNA levels are altered levels in individuals with HIV, playing a dual role in infection. Advances in research have shown that ci-miRNAs could differentiate stages of HIV infection and diseases associated with a viral infection and serve as biomarkers for antiretroviral therapy's effectiveness through changes in their expression. (AU)
Descritores: Infecções por HIV/diagnóstico
MicroRNAs
-Replicação Viral/imunologia
Biomarcadores
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR18.1 - Biblioteca FAMED/HCPA



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