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Pesquisa : G07.775 [Categoria DeCS]
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Id: biblio-951812
Autor: Santos, Alexandra de Andrade; Silveira, Joaquim Albenísio Gomes da; Bonifacio, Aurenivia; Rodrigues, Artenisa Cerqueira; Figueiredo, Márcia do Vale Barreto.
Título: Antioxidant response of cowpea co-inoculated with plant growth-promoting bacteria under salt stress
Fonte: Braz. j. microbiol;49(3):513-521, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Soil salinity is an important abiotic stress worldwide, and salt-induced oxidative stress can have detrimental effects on the biological nitrogen fixation. We hypothesized that co-inoculation of cowpea plants with Bradyrhizobium and plant growth-promoting bacteria would minimize the deleterious effects of salt stress via the induction of enzymatic and non-enzymatic antioxidative protection. To test our hypothesis, cowpea seeds were inoculated with Bradyrhizobium or co-inoculated with Bradyrhizobium and plant growth-promoting bacteria and then submitted to salt stress. Afterward, the cowpea nodules were collected, and the levels of hydrogen peroxide; lipid peroxidation; total, reduced and oxidized forms of ascorbate and glutathione; and superoxide dismutase, catalase and phenol peroxidase activities were evaluated. The sodium and potassium ion concentrations were measured in shoot samples. Cowpea plants did not present significant differences in sodium and potassium levels when grown under non-saline conditions, but sodium content was strongly increased under salt stress conditions. Under non-saline and salt stress conditions, plants co-inoculated with Bradyrhizobium and Actinomadura or co-inoculated with Bradyrhizobium and Paenibacillus graminis showed lower hydrogen peroxide content in their nodules, whereas lipid peroxidation was increased by 31% in plants that were subjected to salt stress. Furthermore, cowpea nodules co-inoculated with Bradyrhizobium and plant growth-promoting bacteria and exposed to salt stress displayed significant alterations in the total, reduced and oxidized forms of ascorbate and glutathione. Inoculation with Bradyrhizobium and plant growth-promoting bacteria induced increased superoxide dismutase, catalase and phenol peroxidase activities in the nodules of cowpea plants exposed to salt stress. The catalase activity in plants co-inoculated with Bradyrhizobium and Streptomyces was 55% greater than in plants inoculated with Bradyrhizobium alone, and this value was remarkably greater than that in the other treatments. These results reinforce the beneficial effects of plant growth-promoting bacteria on the antioxidant system that detoxifies reactive oxygen species. We concluded that the combination of Bradyrhizobium and plant growth-promoting bacteria induces positive responses for coping with salt-induced oxidative stress in cowpea nodules, mainly in plants co-inoculated with Bradyrhizobium and P. graminis or co-inoculated with Bradyrhizobium and Bacillus.
Descritores: Cloreto de Sódio/metabolismo
Bradyrhizobium/fisiologia
Inoculantes Agrícolas/fisiologia
Vigna/microbiologia
Antioxidantes/metabolismo
-Proteínas de Plantas/metabolismo
Estresse Fisiológico
Superóxido Dismutase/metabolismo
Peroxidação de Lipídeos
Catalase/metabolismo
Peroxidase/metabolismo
Estresse Oxidativo
Salinidade
Vigna/crescimento & desenvolvimento
Vigna/metabolismo
Glutationa/metabolismo
Responsável: BR1.1 - BIREME


  2 / 1403 LILACS  
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Id: biblio-974331
Autor: Guo, Ruiting; Wang, Zhiying; Huang, Ying; Fan, Haijuan; Liu, Zhihua.
Título: Biocontrol potential of saline- or alkaline-tolerant Trichoderma asperellum mutants against three pathogenic fungi under saline or alkaline stress conditions
Fonte: Braz. j. microbiol;49(supl.1):236-245, 2018. graf.
Idioma: en.
Projeto: National High Technology Research and Development Program (the 13th Five-Year Plan Program); . Fundamental Research Funds for the Central Universities.
Resumo: ABSTRACT Salinity and alkalinity are major abiotic stresses that limit growth and development of poplar. We investigated biocontrol potential of saline- and alkaline-tolerant mutants of Trichoderma asperellum to mediate the effects of salinity or alkalinity stresses on Populus davidiana × P. alba var. pyramidalis (PdPap poplar) seedlings. A T-DNA insertion mutant library of T. asperellum was constructed using an Agrobacterium tumefaciens mediated transformation system; this process yielded sixty five positive transformants (T1-T65). The salinity tolerant mutant, T59, grew in Potato Dextrose Agar (PDA) containing up to 10% (1709.40 mM) NaCl. Under NaCl-rich conditions, T59 was most effective in inhibiting Alternaria alternata (52.00%). The alkalinity tolerant mutants, T3 and T5, grew in PDA containing up to 0.4% (47.62 mM) NaHCO3. The ability of the T3 and T5 mutants to inhibit Fusarium oxysporum declined as NaHCO3 concentrations increased. NaHCO3 tolerance of the PdPap seedlings improved following treatment with the spores of the WT, T3, and T5 strains. The salinity tolerant mutant (T59) and two alkalinity tolerant mutants (T3 and T5) generated in this study can be applied to decrease the incidence of pathogenic fungi infection under saline or alkaline stress.
Descritores: Doenças das Plantas/microbiologia
Trichoderma/fisiologia
Cloreto de Sódio/metabolismo
Populus/crescimento & desenvolvimento
Álcalis/metabolismo
Alternaria/fisiologia
Antibiose
-Doenças das Plantas/prevenção & controle
Estresse Fisiológico
Trichoderma/genética
Populus/microbiologia
Plântulas/crescimento & desenvolvimento
Plântulas/microbiologia
Responsável: BR1.1 - BIREME


  3 / 1403 LILACS  
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Id: lil-788975
Autor: Orhan, Furkan.
Título: Alleviation of salt stress by halotolerant and halophilic plant growth-promoting bacteria in wheat (Triticum aestivum)
Fonte: Braz. j. microbiol;47(3):621-627, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT In the current study, 18 halotolerant and halophilic bacteria have been investigated for their plant growth promoting abilities in vitro and in a hydroponic culture. The bacterial strains have been investigated for ammonia, indole-3-acetic acid and 1-aminocyclopropane-1-carboxylate-deaminase production, phosphate solubilisation and nitrogen fixation activities. Of the tested bacteria, eight were inoculated with Triticum aestivum in a hydroponic culture. The investigated bacterial strains were found to have different plant-growth promoting activities in vitro. Under salt stress (200 mM NaCl), the investigated bacterial strains significantly increased the root and shoot length and total fresh weight of the plants. The growth rates of the plants inoculated with bacterial strains ranged from 62.2% to 78.1%.Identifying of novel halophilic and halotolerant bacteria that promote plant growth can be used as alternatives for salt sensitive plants. Extensive research has been conducted on several halophilic and halotolerant bacterial strains to investigate their plant growth promoting activities. However, to the best of my knowledge, this is the first study to inoculate these bacterial strains with wheat.
Descritores: Reguladores de Crescimento de Planta/biossíntese
Estresse Fisiológico
Bactérias/efeitos dos fármacos
Bactérias/metabolismo
Triticum/fisiologia
Triticum/microbiologia
Fenômenos Fisiológicos Bacterianos
Salinidade
-Fenótipo
Raízes de Plantas/fisiologia
Raízes de Plantas/microbiologia
Biomassa
Amônia/metabolismo
Fixação de Nitrogênio
Responsável: BR1.1 - BIREME


  4 / 1403 LILACS  
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Id: biblio-1049133
Autor: Paiva, Emanoela Pereira de; Torres, Salvador Barros; Oliveira, Renata Ramayane Torquato; Sá, Francisco Vanies da Silva; Leite, Moadir de Sousa; Alves, Tatianne Raianne Costa; Oliveira, Daniela Marques de.
Título: Germination and osmotic adjustment in Salvia hispanica L. (Lamiaceae) seedlings under water and thermal stress / Germinação e ajustamento osmótico em plântulas de Salvia hispanica L. (lamiaceae) sob estresse hídrico e térmico
Fonte: Biosci. j. (Online);35(6):1829-1838, nov./dec. 2019. graf.
Idioma: en.
Resumo: Salvia hispanica seedlings have stood out for their potentialities in nutrients for agri-food industries. Nevertheless, studies related to this species are still scarce, especially with respect to agronomic factors that enable its propagation and development. Hence, this study aimed to evaluate germination, vigor and osmotic adjustment of S. hispanica seeds and seedlings subjected to water and thermal stress. The experimental design was completely randomized, with treatments arranged in factorial scheme, corresponding to five levels of polyethylene glycol (PEG 6000) (0.0; -0.1; -0.2; -0.3 and -0.4 MPa) and five temperatures (20, 25, 30 and 20-30 °C), with four replicates of 50 seeds in each treatment. Germination test was set on blotter paper, moistened with PEG 6000 solutions at the previously mentioned potentials, under 8-h photoperiod. The following variables were analyzed: germination, germination speed index, seedling shoot length, seedling root length, seedling dry matter and contents of chlorophyll, carotenoids, amino acids, proline and sugars. Polyethylene glycol levels of -0.4 MPa at all temperatures and -0.3 MPa at 30 °C and 20-30 °C enable germination and vigor of S. hispanica seedlings. S. hispanica seedlings are able to perform osmotic adjustment under water stress conditions up to the level of -0.3 MPa, when grown from seeds germinated at temperatures of up to 25 ºC.

As sementes de S. hispanica têm se destacado pela sua potencialidade em nutrientes para as indústrias agroalimentares. No entanto, ainda são escassas as pesquisas relacionadas a esta espécie, principalmente no tocante aos fatores agronômicos que viabilizem a sua propagação e desenvolvimento. Com isso, objetivou-se verificar a germinação, vigor e ajustamento osmótico de sementes e plântulas de S. hispanicasubmetidas aos estresses hídrico e térmico. O delineamento experimental foi o inteiramente casualizado, com tratamentos distribuídos em esquema fatorial, correspondente a cinco níveis de polietileno glicol (PEG 6000) (0,0; -0,1; -0,2; -0,3 e -0,4 MPa) e quatro temperaturas (20, 25, 30 e 20-30 °C), com quatro repetições de 50 sementes para cada tratamento. Para isso, instalou-se o teste de germinação em substrato mata-borrão, umedecidos com soluções de PEG 6000 nos potenciais mencionados, sob fotoperíodo de 8 horas de luz. As variáveis analisadas foram germinação, índice de velocidade de germinação, comprimento de parte aérea e das raízes das plântulas, massa seca de plântulas, teores de clorofila e carotenoides, aminoácidos, prolina e açúcares. O nível de polietilenoglicol de -0,4 MPa para todas as temperaturas estudadas, e -0,3 MPa nas temperaturas de 30 °C e 20-30 °C inviabilizam a germinação e o vigor das plântulas de S. hispanica. As plântulas de S. hispanica são capazes de realizar ajuste osmótico sob condições de estresse hídrico até os níveis de -0,3 MPa, quando provenientes de sementes germinadas em temperaturas de até 25 ºC.
Descritores: Polietilenoglicóis
Germinação
Resposta ao Choque Térmico
Salvia
Desidratação
-Estresse Fisiológico
Responsável: BR396.1 - Biblioteca Central


  5 / 1403 LILACS  
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Id: lil-618676
Autor: Delgado Maidana, Walter; Vega Carduz, E; Sanabria, L; Figueredo Thiel, Susy Jacqueline.
Título: Prevalencia del síndrome de Burnout en médicos anestesiólogos del Paraguay durante el año 2010 / Prevalence of Burnout syndrome in anesthesiologists of Paraguay during 2010
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);9(1):13-20, jun. 2011. tab.
Idioma: es.
Resumo: El síndrome de Burnout constituye un tipo de estrés laboral que se observa en profesionales de la salud que mantienen contacto directo y constante con otras personas. Para determinar su prevalencia en los médicos anestesiólogos del Paraguay, durante el 2010, se realizó un estudio observacional, descriptivo y de corte transverso en 146 médicos anestesiólogos del Paraguay mediante el cuestionario Maslash Burnout Inventory que evalúa las dimensiones del síndrome. Se identificó una prevalencia global de síndrome de Burnout de 72,6%. En el grupo de médicos residentes la prevalencia fue 88,6% (x2= 8,14 P<0,005). El cansancio emocional fue la dimensión más frecuente 60,9%. Los menores de 35 años padecieron el síndrome en mayor número 83,1% (x2= 8,14 P<0,005). Tener una pareja estable se identificó como un factor de protección (x2= 3,77 P<0,05). No se identificó relación significativa con el sexo y lugares de trabajo (medicina pública y privada). La prevalencia hallada fue alta en comparación a otros estudios. Ser médico residente, menor de 35 años, no tener una pareja estable y con sobrecarga de trabajo son los factores más frecuentes encontrados en el Síndrome de Burnout en los anestesiólogos del Paraguay.

Burnout Syndrome is a type of occupational stress observed health professionals who keep direct and constant contact with other persons. To determine its prevalence in anesthesiologist in 2010, this cross-sectional descriptive study was carried out in 146 Paraguayan anesthesiologist using the Maslash Burnout Inventory. This instrument evaluates the dimensions of the syndrome. A global prevalence of BS 72.6% was found. In the group of resident physician we found a prevalence of 88.6% (x2= 8.14 P<0.005). The most frequent dimension was the emotional exhaustion (60.9%). The syndrome was more frequent in the anesthesiologsts under 35 years of age (83.1%; x2= 8.14 P<0.005). Having a stable partner was identified as a protective factor (x2= 3.77 P<0.05). No significant relation was found with sex and working places (public and private medicine). The prevalence of BS found was high in comparison to other studies. Being a resident physician, under 35 year old, not having a stable partner and overload of work are the factors most frequently found in the Burnout Syndrome in Paraguayan anesthesiologysts.
Descritores: Anestesia
Estresse Fisiológico
Prevalência
Responsável: PY3.1 - Biblioteca


  6 / 1403 LILACS  
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Id: biblio-1018622
Autor: Gonzàlez Zarza, Ruth Liliana(edt).
Título: Estrès psicosocial como factor predisponente de patologìa aguda en una poblaciòn rural / Psychosocial stress as predisposing factors for acute conditions in a rural.
Fonte: ASUNCIÒN; IPS/UCA; 00112009. 44 p. graf.
Idioma: es.
Resumo: Este trabajo pretende evaluar la relaciòn que existe entre estrès psicosocial y enfermedad aguda.
Descritores: Estresse Fisiológico/complicações
Estresse Psicológico/psicologia
Estresse Psicológico/reabilitação
-Paraguai
Responsável: PY94.1 - Biblioteca
PY94.1; bips2, Gon, 2010


  7 / 1403 LILACS  
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Id: biblio-839372
Autor: Mejía-Barajas, Jorge A; Montoya-Pérez, Rocío; Salgado-Garciglia, Rafael; Aguilera-Aguirre, Leopoldo; Cortés-Rojo, Christian; Mejía-Zepeda, Ricardo; Arellano-Plaza, Melchor; Saavedra-Molina, Alfredo.
Título: Oxidative stress and antioxidant response in a thermotolerant yeast
Fonte: Braz. j. microbiol;48(2):326-332, April.-June 2017. tab, graf.
Idioma: en.
Resumo: Abstract Stress tolerance is a key attribute that must be considered when using yeast cells for industrial applications. High temperature is one factor that can cause stress in yeast. High environmental temperature in particular may exert a natural selection pressure to evolve yeasts into thermotolerant strains. In the present study, three yeasts (Saccharomyces cerevisiae, MC4, and Kluyveromyces marxianus, OFF1 and SLP1) isolated from hot environments were exposed to increased temperatures and were then compared with a laboratory yeast strain. Their resistance to high temperature, oxidative stress, and antioxidant response were evaluated, along with the fatty acid composition of their cell membranes. The SLP1 strain showed a higher specific growth rate, biomass yield, and biomass volumetric productivity while also showing lower duplication time, reactive oxygen species (ROS) production, and lipid peroxidation. In addition, the SLP1 strain demonstrated more catalase activity after temperature was increased, and this strain also showed membranes enriched in saturated fatty acids. It is concluded that the SLP1 yeast strain is a thermotolerant yeast with less oxidative stress and a greater antioxidant response. Therefore, this strain could be used for fermentation at high temperatures.
Descritores: Saccharomyces cerevisiae/fisiologia
Estresse Fisiológico
Kluyveromyces/fisiologia
Estresse Oxidativo
Antioxidantes/metabolismo
-Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/efeitos da radiação
Saccharomyces cerevisiae/química
Kluyveromyces/crescimento & desenvolvimento
Kluyveromyces/efeitos da radiação
Kluyveromyces/química
Peroxidação de Lipídeos
Catalase/análise
Membrana Celular/química
Espécies Reativas de Oxigênio/metabolismo
Biomassa
Ácidos Graxos/análise
Temperatura Alta
Responsável: BR1.1 - BIREME


  8 / 1403 LILACS  
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Id: biblio-839369
Autor: Reis, Vanda Renata; Antonangelo, Ana Teresa Burlamaqui Faraco; Bassi, Ana Paula Guarnieri; Colombi, Débora; Ceccato-Antonini, Sandra Regina.
Título: Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance
Fonte: Braz. j. microbiol;48(2):268-274, April.-June 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo a Pesquisa do Estado de São Paulo.
Resumo: Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cerevisiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive.
Descritores: Saccharomyces cerevisiae/fisiologia
Saccharomyces cerevisiae/genética
Variação Genética
Repetições de Microssatélites
Etanol/metabolismo
-Fenótipo
Saccharomyces cerevisiae/classificação
Saccharomyces cerevisiae/metabolismo
Estresse Fisiológico
Genótipo
Glucose/metabolismo
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME


  9 / 1403 LILACS  
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Id: biblio-839367
Autor: Santos, Silvana Gomes dos; Silva, Paula Renata Alves da; Garcia, Andres Calderin; Zilli, Jerri Édson; Berbara, Ricardo Luis Louro.
Título: Dark septate endophyte decreases stress on rice plants
Fonte: Braz. j. microbiol;48(2):333-341, April.-June 2017. tab, graf.
Idioma: en.
Projeto: Embrapa; . Fundação de Amparo à Pesquisa no Estado do Rio de Janeiro; . National Council for Scientific and Technological Development; . Fundação de Amparo à Pesquisa de Minas Gerais.
Resumo: Abstract Abiotic stress is one of the major limiting factors for plant development and productivity, which makes it important to identify microorganisms capable of increasing plant tolerance to stress. Dark septate endophytes can be symbionts of plants. In the present study, we evaluated the ability of dark septate endophytes isolates to reduce the effects of water stress in the rice varieties Nipponbare and Piauí. The experiments were performed under gnotobiotic conditions, and the water stress was induced with PEG. Four dark septate endophytes were isolated from the roots of wild rice (Oryza glumaepatula) collected from the Brazilian Amazon. Plant height as well as shoot and root fresh and dry matter were measured. Leaf protein concentrations and antioxidant enzyme activity were also estimated. The dark septate endophytes were grown in vitro in Petri dishes containing culture medium; they exhibited different levels of tolerance to salinity and water stress. The two rice varieties tested responded differently to inoculation with dark septate endophytes. Endophytes promoted rice plant growth both in the presence and in the absence of a water deficit. Decreased oxidative stress in plants in response to inoculation was observed in nearly all inoculated treatments, as indicated by the decrease in antioxidant enzyme activity. Dark septate endophytes fungi were shown to increase the tolerance of rice plants to stress caused by water deficiency.
Descritores: Oryza/fisiologia
Oryza/microbiologia
Estresse Fisiológico
Desidratação
Endófitos/crescimento & desenvolvimento
-Proteínas de Plantas/análise
Oryza/enzimologia
Brasil
Raízes de Plantas/microbiologia
Endófitos/isolamento & purificação
Antioxidantes/análise
Responsável: BR1.1 - BIREME


  10 / 1403 LILACS  
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Id: lil-751378
Autor: Bruder-Nascimento, Thiago; Silva, Samuel T; Boer, Patrícia A; Cordellini, Sandra.
Título: Effects of exercise training on stress-induced vascular reactivity alterations: role of nitric oxide and prostanoids
Fonte: Braz. j. phys. ther. (Impr.) = Rev. bras. fisioter;19(3):177-185, May-Jun/2015. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Background: Physical exercise may modify biologic stress responses. Objective: To investigate the impact of exercise training on vascular alterations induced by acute stress, focusing on nitric oxide and cyclooxygenase pathways. Method: Wistar rats were separated into: sedentary, trained (60-min swimming, 5 days/week during 8 weeks, carrying a 5% body-weight load), stressed (2 h-immobilization), and trained/stressed. Response curves for noradrenaline, in the absence and presence of L-NAME or indomethacin, were obtained in intact and denuded aortas (n=7-10). Results: None of the procedures altered the denuded aorta reactivity. Intact aortas from stressed, trained, and trained/stressed rats showed similar reduction in noradrenaline maximal responses (sedentary 3.54±0.15, stressed 2.80±0.10*, trained 2.82±0.11*, trained/stressed 2.97± 0.21*, *P<0.05 relate to sedentary). Endothelium removal and L-NAME abolished this hyporeactivity in all experimental groups, except in trained/stressed rats that showed a partial aorta reactivity recovery in L-NAME presence (L-NAME: sedentary 5.23±0,26#, stressed 5.55±0.38#, trained 5.28±0.30#, trained/stressed 4.42±0.41, #P<0.05 related to trained/stressed). Indomethacin determined a decrease in sensitivity (EC50) in intact aortas of trained rats without abolishing the aortal hyporeactivity in trained, stressed, and trained/stressed rats. Conclusions: Exercise-induced vascular adaptive response involved an increase in endothelial vasodilator prostaglandins and nitric oxide. Stress-induced vascular adaptive response involved an increase in endothelial nitric oxide. Beside the involvement of the endothelial nitric oxide pathway, the vascular response of trained/stressed rats involved an additional mechanism yet to be elucidated. These findings advance on the understanding of the vascular processes after exercise and stress alone and in combination. .
Descritores: Condicionamento Físico Animal
Estresse Fisiológico
Vasos Sanguíneos/fisiologia
Prostaglandinas/fisiologia
Óxido Nítrico/fisiologia
-Ratos Wistar
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME



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