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Id: biblio-896971
Autor: Oliveira, Fabiana da Rocha; Noronha, Maria das Dores Nogueira; Lozano, Jorge Luis Lopez.
Título: Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
Fonte: Rev. Soc. Bras. Med. Trop;50(3):365-373, May-June 2017. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. METHODS: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. RESULTS AND CONCLUSIONS: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice.
Descritores: Elapidae
Venenos Elapídicos/enzimologia
Venenos Elapídicos/genética
Venenos Elapídicos/química
Fosfolipases A2/metabolismo
-Reações Cruzadas
Eletroforese
Fosfolipases A2/química
Dose Letal Mediana
Camundongos Endogâmicos BALB C
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-950759
Autor: Fakruddin, Md; Rahaman, Mizanur; Ahmed, Monzur Morshed; Hoque, Md Mahfuzul.
Título: Stress tolerant virulent strains of Cronobacter sakazakii from food
Fonte: Biol. Res;47:1-12, 2014. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was to isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh. RESULT: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical characteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp. specific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately high antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates were capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression, haemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant resistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them produced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with other gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri and Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress. CONCLUSION: Special attention should be given in ensuring hygiene in production and post-processing to prevent contamination of food with such stress-tolerant virulent Cronobacter sakazakii.
Descritores: Estresse Fisiológico/fisiologia
Cronobacter sakazakii/fisiologia
Leite/microbiologia
Microbiologia de Alimentos
-Bangladesh
Virulência
DNA Bacteriano/análise
Resistência a Tetraciclina/genética
Reação em Cadeia da Polimerase/métodos
Especiarias/microbiologia
Sideróforos/metabolismo
Análise de Sequência de DNA
Primers do DNA
Reações Cruzadas
Cronobacter sakazakii/isolamento & purificação
Cronobacter sakazakii/classificação
Cronobacter sakazakii/patogenicidade
Leite/classificação
Eletroforese em Gel de Poliacrilamida
Fermentação/fisiologia
Temperatura Alta
Concentração de Íons de Hidrogênio
Antibacterianos/farmacologia
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Carvalho, Maria Esther de
ANDRADE JUNIOR, Heitor Franco de
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Id: biblio-1041484
Autor: Santos, Anderson Pacheco dos; Carvalho, Maria Esther de; Meirelles, Luciana Regina; Andrade Junior, Heitor Franco de.
Título: Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys
Fonte: Rev. Soc. Bras. Med. Trop;51(5):665-669, Sept.-Oct. 2018. graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. RESULTS: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. CONCLUSIONS: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays.
Descritores: Ureia/farmacologia
Imunoglobulina G/sangue
Anticorpos Antiprotozoários/sangue
Leishmaniose/imunologia
Doença de Chagas/imunologia
Reações Cruzadas/imunologia
Afinidade de Anticorpos/imunologia
-Ureia/química
Brasil/epidemiologia
Ensaio de Imunoadsorção Enzimática
Biomarcadores/química
Leishmaniose/complicações
Leishmaniose/diagnóstico
Leishmaniose/epidemiologia
Vigilância da População
Sensibilidade e Especificidade
Doença de Chagas/complicações
Doença de Chagas/diagnóstico
Doença de Chagas/epidemiologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Coelho, Paulo Marcos Zech
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Id: biblio-1041506
Autor: Gusmão, Michélia Antônia do Nascimento; Emídio, Nayara Braga; Marconato, Danielle Gomes; Farani, Priscila Silva Grijó; Maniezzi, Luiz Felipe; Coelho, Paulo Marcos Zech; Oliveira, Rodrigo Correa; Vasconcelos, Eveline Gomes; Faria-Pinto, Priscila de.
Título: IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180139, 2019. graf.
Idioma: en.
Projeto: Conselho Nacional de Pesquisa (CNPq).
Resumo: Abstract INTRODUCTION: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins. METHODS: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity. RESULTS: Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production. CONCLUSIONS: Potato apyrase and SmATPDases have IgE-binding epitopes.
Descritores: Apirase/imunologia
Schistosoma mansoni/imunologia
Esquistossomose mansoni/imunologia
Solanum tuberosum/enzimologia
Imunoglobulina E/imunologia
Anticorpos Anti-Helmínticos/imunologia
Epitopos/imunologia
-Ensaio de Imunoadsorção Enzimática
Western Blotting
Reações Cruzadas
Camundongos Endogâmicos C57BL
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


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Id: lil-542257
Autor: Guzmán Meléndez, María Antonieta; Marinovic Mayorga, María Angélica; Salinas Luypaert, Jessica; Toche Pinaud, Paola.
Título: Síndrome látex-fruta en un adolescente de 13 años: evolución clínica y nuevas sensibilizaciones en dos años de observación / Latex-Fruit Syndrome in a 13 years-old teenager: clinical evolution and new sensitizations durig two years
Fonte: Arch. alerg. inmunol. clin;38(1):36-39, 2007. tab.
Idioma: es.
Descritores: Hipersensibilidade Alimentar
Hipersensibilidade ao Látex
-Reações Cruzadas
Limites: Humanos
Masculino
Adolescente
Tipo de Publ: Relatos de Casos
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Id: biblio-1177504
Autor: Zuluaga, C; Lara, J; Berrouet, MC.
Título: Síndrome de Guillain-Barré asociado a accidente apídico: reporte de caso / Guillain-Barré syndrome associated with a bee sting accident: a case report / Síndrome de Guillain-Barré associada a acidente elapídico: relato de caso
Fonte: Med. U.P.B = Med. UPB;40(1):82-85, 03/03/2021. tab.
Idioma: es.
Resumo: El accidente apídico implica un bajo riesgo de morir por reacciones agudas como anafilaxia. Es frecuente que se desconozcan otros riesgos como el hepático y el neurológico. Dentro de las complicaciones menos conocidas está la polineuropatía aguda conocida como Guillain- Barré. La causa más ampliamente descrita es la reacción cruzada entre IgE y la proteína básica de mielina. A continuación, se reporta el primer caso en América Latina sobre la asociación entre Guillain-Barré y accidente por picadura de abejas. En este caso, hay criterios de causalidad como el nexo temporal, la plausibilidad biológica y la coherencia con otros artículos reportados en la literatura médica.

Bee sting accident usually implies a low risk of dying from acute reactions such as anaphylaxis. Other risks such as liver and neurological risks are often unknown. Among the lesser-known complications caused by this type of accident is the acute polyneuropathy known as Guillain-Barré. The most widely described cause is the cross reaction between IgE and myelin basic protein. The article reports the first case in Latin America on the association between Guillain-Barré and a bee sting accident. In this case, there are causality criteria such as temporal link, biological plausibility, and consistency with other articles reported in the medical literature

O acidente elapídico implica um baixo risco de morte por reações agudas, como anafilaxia. Outros riscos, como hepáticos e neurológicos, são frequentemente desconhecidos. Entre as complicações menos conhecidas está a polineuropatia aguda conhecida como Guillain-Barré. A causa mais amplamente descrita é a reação cruzada entre a IgE e a proteína básica da mielina. A seguir, é relatado o primeiro caso na América Latina sobre a associação entre Guillain-Barré e um acidente com picada de abelha. Nesse caso, há critérios de causalidade como vínculo temporal, plausibilidade biológica e consistência com outros artigos relatados na literatura médica
Descritores: Síndrome de Guillain-Barré
-Polineuropatias
Abelhas
Mordeduras e Picadas
Imunoglobulina E
Acidentes
Reações Cruzadas
Morte
Anafilaxia
Fígado
Limites: Humanos
Animais
Tipo de Publ: Relatos de Casos
Responsável: CO101.1 - BCdeS - Biblioteca Ciencias de la Salud


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AVILA, Priscilla Elisangela
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Id: lil-324504
Autor: Avila, Priscilla Elisangela; Kirchgatter, Karin; Brunialti, Karen Cristina S; Oliveira, Alessandra M; Siciliano, Rinaldo F; Di Santi, Silvia Maria.
Título: Evaluation of a rapid dipstick test, Malar-CheckTM, for the diagnosis of Plasmodium falciparum malaria in Brazil
Fonte: Rev. Inst. Med. Trop. Säo Paulo;44(5):293-296, Oct. 2002. ilus, tab.
Idioma: en.
Resumo: The present study was carried out to evaluate the Malar-CheckTM Pf test, an immunochromatographic assay that detects Plasmodium falciparum Histidine Rich Protein II, does not require equipment, and is easy and rapid to perform. In dilution assays performed to test sensitivity against known parasite density, Malar-CheckTMwere compared with thick blood smear (TBS), the gold standard for diagnosis. Palo Alto isolate or P. falciparum blood from patients with different parasitemias was used. The average cut-off points for each technique in three independent experiments were 12 and 71 parasites/mm³ (TBS and Malar-CheckTM, respectively). In the field assays, samples were collected from patients with fever who visited endemic regions. Compared to TBS, Malar-CheckTMyielded true-positive results in 38 patients, false-positive results in 3, true-negative results in 23, and false-negative result in 1. Malar-CheckTMperformed with samples from falciparum-infected patients after treatment showed persistence of antigen up to 30 days. Malar-CheckTM should aid the diagnosis of P. falciparum in remote areas and improve routine diagnosis even when microscopy is available. Previous P. falciparum infection, which can determine a false-positive test in cured individuals, should be considered. The prompt results obtained with the Malar-CheckTM for early diagnosis could avoid disease evolution to severe cases
Descritores: Imunoensaio
Cromatografia
Malária Falciparum
-Plasmodium falciparum
Kit de Reagentes para Diagnóstico
Brasil
Sensibilidade e Especificidade
Reações Cruzadas
Estudo de Avaliação
Reações Falso-Positivas
Antígenos de Protozoários
Limites: Animais
Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: lil-320648
Autor: Vexenat, Ana de Cassia; Santana, Jaime M; Teixeira, Antônio R. L.
Título: Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (viannia) braziliensis
Fonte: Rev. Inst. Med. Trop. Säo Paulo;38(3):177-185, May-Jun. 1996.
Idioma: en.
Resumo: We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100 positive results by ELISA when the Leishmania braziliensis and Leishmania chagasi antigens were used, respectively. The Kala-azar sera showed 100 positive results with Trypanosoma cruzi or L. braziliensis antigens by immunofluorescence assays. The antibodies in the sera of Mucocutaneous leishmaniasis patients showed 100 positive results by ELISA assays with T. cruzi or L. chagasi antigens. Furthermore, the direct agglutination of L. chagasi promastigotes showed that 95 of Kala-azar and 35 of Mucocutaneous leishmaniasis sera agglutinated the parasite in dilutions above 1:512. In contrast, 15 of Chagas sera agglutinated the parasite in dilutions 1:16 and below. Western blot analysis showed that the Chagas sera that formed at least 24 bands with the T. cruzi also formed 13 bands with the L. chagasi and 17 bands with the L. braziliensis. The Kala-azar sera that recognized at least 29 bands with the homologous antigen also formed 14 bands with the T. cruzi and 10 bands with the L. braziliensis antigens. Finally, the Mucocutaneous leishmaniasis sera that formed at least 17 bands with the homologous antigen also formed 10 bands with the T. cruzi and four bands with the L. chagasi antigens. These results indicate the presence of common antigenic determinants in several protozoal proteins and, therefore, explain the serologic cross-reactions reported here.
Descritores: Leishmaniose Visceral
Anticorpos Antiprotozoários/imunologia
Doença de Chagas/imunologia
Leishmaniose Mucocutânea/imunologia
-Trypanosoma cruzi
Leishmania braziliensis
Leishmania infantum
Leishmaniose Visceral
Anticorpos Antiprotozoários/sangue
Doença de Chagas/sangue
Leishmaniose Mucocutânea/sangue
Reações Cruzadas
Limites: Humanos
Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-326238
Autor: Picolo, G; Chacur, M; Gutiérrez, J. M; Teixeira, C. F. P; Cury, Y.
Título: Evaluation of antivenoms in the neutralization of hyperalgesia and edema induced by Bothrops jararaca and Bothrops asper snake venoms
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;35(10):1221-1228, Oct. 2002. graf.
Idioma: en.
Resumo: Neutralization of hyperalgesia induced by Bothrops jararaca and B. asper venoms was studied in rats using bothropic antivenom produced at Instituto Butantan (AVIB, 1 ml neutralizes 5 mg B. jararaca venom) and polyvalent antivenom produced at Instituto Clodomiro Picado (AVCP, 1 ml neutralizes 2.5 mg B. aspar venom). The intraplantar injection of B. jararaca and B. asper venoms caused hyperalgesia, which peaked 1 and 2 h after injection, respectively. Both venoms also induced edema with a similar time course. When neutralization assays involving the independent injection of venom and antivenom were performed, the hyperalgesia induced by B. jararaca venom was neutralized only when bothropic antivenom was administered iv 15 min before venom injection, whereas edema was neutralized when antivenom was injected 15 min or immediately before venom injection. On the other hand, polyvalent antivenom did not interfere with hyperalgesia or edema induced by B. asper venom, even when administered prior to envenomation. The lack of neutralization of hyperalgesia and edema induced by B. asper venom is not attributable to the absence of neutralizing antibodies in the antivenom, since neutralization was achieved in assays involving preincubation of venom and antivenom. Cross-neutralization of AVCP or AVIB against B. jararaca and B. asper venoms, respectively, was also evaluated. Only bothropic antivenom partially neutralized hyperalgesia induced by B. asper venom in preincubation experiments. The present data suggest that hyperalgesia and edema induced by Bothrops venoms are poorly neutralized by commercial antivenoms even when antibodies are administered immediately after envenomation
Descritores: Antivenenos
Bothrops
Venenos de Crotalídeos
Edema
Hiperalgesia
-Testes de Neutralização
Ratos Wistar
Reações Cruzadas
Estudo de Avaliação
Edema
Hiperalgesia
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1096405
Autor: Sarraquigne, María P; Mariño, Andrea I; Saranz, Ricardo; Colella, Mauricio; López, Karina; Bovina Martijena, María del P; Parisi, Claudio; Orellana, Julio; Agüero, Claudio; Seisdedos, Viviana; Lozano, Alejandro; Máspero, Jorge F; Boudet, Raúl V; Suárez García, Juan M; Bandín, Gloria; García, Marcela; Gervasoni, María E; Luconi, Natalia; Matta Rufolo, Mónica; Skrie, Víctor; Bózzola, Martín; Procopio, Norberto; Sánchez Segovia, Antonio; Araoz, Irene; Cassaniti, María C; Mindel, Elsa.
Título: Alergia e intolerancia a antiinflamatorios no esteroideos en pediatría / Allergy and intolerance to nonsteroidal anti-inflammatory drugs in children
Fonte: Arch. argent. pediatr;118(1):S1-S11, 2020-02-00. ilus, tab.
Idioma: es.
Resumo: Los antiinflamatorios no esteroideos son ampliamente recetados en niños. Constituyen la segunda causa de reacciones a medicamentos en pediatría después de los antibióticos betalactámicos; sin embargo, solo una parte de estas son reacciones de hipersensibilidad. La prevalencia de dichas reacciones a antiinflamatorios no esteroideos en niños es del 0,3 % y aumenta al 5 % en asmáticos.Los mecanismos fisiopatológicos involucrados (inhibición de la ciclooxigenasa, hipersensibilidad mediada por inmunoglobulina E, linfocitos T reactivos y/o afectación de la inmunidad innata) darán lugar a diferentes entidades clínicas con sintomatología dispar.La confusión con síntomas propios de procesos virales y la variabilidad clínica hacen del diagnóstico de certeza un verdadero desafío. Una historia clínica detallada, análisis de laboratorio, pruebas cutáneas y de provocación controlada permitirán definir estrategias para cada paciente en particular sin etiquetar como alérgico a un niño que no lo es ni exponer a riesgos innecesarios a quien está sensibilizado.

Nonsteroidal anti-inflammatory drugs are widely prescribed in children. They are the second cause of drug ́s reactions in pediatrics after beta-lactam antibiotics, however only a part of them are hypersensitivity reactions. The prevalence of these reactions to nonsteroidal anti-inflammatory drugs in children is 0.3 %, increasing to 5 % in asthmatics.The different physiopathological mechanisms involved (inhibition of cyclooxygenase, immunoglobulin E-mediated hypersensitivity, reactive T lymphocytes and/or disturbance of innate immunity) will cause different clinical entities with diverse symptoms.The confusion between the common symptoms of a viral infection and a hypersensitivity reaction, and the variability of the clinical presentations make diagnosis a real challenge.A detailed clinical history, laboratory, skin and controlled provocation tests will provide strategies for each patient, without labeling a child who is not an allergic one, or taking unnecessary risks with those who are sensitized.
Descritores: Anti-Inflamatórios não Esteroides
Hipersensibilidade a Drogas/diagnóstico
Hipersensibilidade a Drogas/tratamento farmacológico
-Testes Cutâneos
Reações Cruzadas
Hipersensibilidade a Drogas/prevenção & controle
Limites: Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Adolescente
Tipo de Publ: Conferência de Consenso
Guia de Prática Clínica
Responsável: AR94.1 - Centro de Información Pediatrica



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