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Pesquisa : G16.012 [Categoria DeCS]
Referências encontradas : 71 [refinar]
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Id: biblio-1291638
Autor: Paiders, Matiss; Nikolajeva, Vizma; Makarenkova, Galina; Orola, Liana; Dimanta, Ilze; Kleperis, Janis.
Título: Changes in freshwater sediment microbial populations during fermentation of crude glycerol
Fonte: Electron J Biotechnol;49:34-41, Jan. 2021. tab, graf.
Idioma: en.
Projeto: Latvian Council of Science NN-CARMA.
Resumo: BACKGROUND: This work studied how the exposure to an unusual substrate forced a change in microbial populations during anaerobic fermentation of crude glycerol, a by-product of biodiesel production, with freshwater sediment used as an inoculum. RESULTS: The microbial associations almost completely (99.9%) utilized the glycerol contained in crude glycerol 6 g L 1 within four days, releasing gases, organic acids (acetic, butyric) and alcohols (ethanol, n-butanol) under anaerobic conditions. In comparison with control medium without glycerol, adding crude glycerol to the medium increased the amount of ethanol and n-butanol production and it was not significantly affected by incubation temperature (28 C or 37 C), nor incubation time (4 or 8 d), but it resulted in reduced amount of butyric acid. Higher volume of gas was produced at 37 C despite the fact that the overall bacterial count was smaller than the one measured at 20 C. Main microbial phyla of the inoculum were Actinobacteria, Proteobacteria and Firmicutes. During fermentation, significant changes were observed and Firmicutes, especially Clostridium spp., began to dominate, and the number of Actinobacteria and Gammaproteobacteria decreased accordingly. Concentration of Archaea decreased, especially in medium with crude glycerol. These changes were confirmed both by culturing and culture-independent (concentration of 16S rDNA) methods. CONCLUSIONS: Crude glycerol led to the adaptation of freshwater sediment microbial populations to this substrate. Changes of microbial community were a result of a community adaptation to a new source of carbon.
Descritores: Bactérias/isolamento & purificação
Sedimentos Geológicos/microbiologia
Água Doce/microbiologia
Glicerol/metabolismo
-Bactérias/metabolismo
Adaptação Biológica
Biocombustíveis
Fermentação
Reação em Cadeia da Polimerase em Tempo Real/métodos
Anaerobiose
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-843176
Autor: Chávez-Ambriz, Lluvia A; Hernández-Morales, Alejandro; Cabrera-Luna, José A; Luna-Martínez, Laura; Pacheco-Aguilar, Juan R.
Título: Aislados de Bacillus provenientes de la rizósfera de cactus incrementan la germinación y la floración en Mammillaria spp. (Cactaceae) / Bacillus isolates from rhizosphere of cacti improve germination and bloom in Mammillaria spp. (Cactaceae)
Fonte: Rev. argent. microbiol;48(4):333-341, dic. 2016. ilus, tab.
Idioma: es.
Resumo: Las cactáceas son la vegetación característica de las zonas áridas en México, donde las lluvias son escasas, la evapotranspiración es elevada y la fertilidad de los suelos es baja. Las plantas han desarrollado estrategias fisiológicas como la asociación con microorganismos en la zona de la rizósfera para incrementar la captación de nutrientes. En el presente trabajo se obtuvieron 4 aislados bacterianos de la rizósfera de Mammillaria magnimamma y Coryphantha radians, los que fueron nombrados como QAP3, QAP19, QAP22 y QAP24 e identificados genéticamente como pertenecientes al género Bacillus. Estos aislados exhibieron in vitro propiedades bioquímicas como solubilización de fosfatos, producción de ácido indolacético y actividad ACC deaminasa, que se relacionan con la promoción del crecimiento de las plantas. Dicha promoción fue ensayada inoculando semillas de M. magnimamma y evaluando luego algunos parámetros. Se encontró que todos los aislados incrementaron la germinación desde un 17% hasta un 34,3% (con respecto a las semillas testigo sin inocular); el aislado QAP24 fue el que presentó el mayor efecto en este sentido y permitió la germinación de todas las semillas viables (84,7%) 3 días antes que en el testigo. La inoculación de este aislado en plantas de Mammillaria zeilmanniana mostró un efecto positivo sobre la floración: en 2 meses dentro del período de un año se detectó un incremento en el número de plantas en floración con respecto a las plantas testigo, de hasta el 31,0% en uno de ellos. Se concluye que los aislados de Bacillus spp. caracterizados poseen potencial para ser empleados en programas de conservación de especies vegetales de zonas áridas.

Cacti are the most representative vegetation of arid zones in Mexico where rainfall is scarce, evapotranspiration is high and soil fertility is low. Plants have developed physiological strategies such as the association with microorganisms in the rhizosphere zone to increase nutrient uptake. In the present work, four bacterial isolates from the rhizosphere of Mammillaria magnimamma and Coryphantha radians were obtained and named as QAP3, QAP19, QAP22 and QAP24, and were genetically identified as belonging to the genus Bacillus, exhibiting in vitro biochemical properties such as phosphate solubilization, indoleacetic acid production and ACC deaminase activity related to plant growth promotion, which was tested by inoculating M. magnimamma seeds. It was found that all isolates increased germination from 17 to 34.3% with respect to the uninoculated control seeds, being QAP24 the one having the greatest effect, accomplishing the germination of viable seeds (84.7%) three days before the control seeds. Subsequently, the inoculation of Mammillari zeilmanniana plants with this isolate showed a positive effect on bloom, registering during two months from a one year period, an increase of up to 31.0% in the number of flowering plants compared to control plants. The characterized Bacillus spp. isolates have potential to be used in conservation programs of plant species from arid zones.
Descritores: Bacillus/isolamento & purificação
Bacillus/classificação
Adaptação Biológica/fisiologia
Conservação dos Recursos Naturais/métodos
Cactaceae/microbiologia
Rizosfera
Inoculantes Agrícolas/crescimento & desenvolvimento
-Germinação/efeitos dos fármacos
Flores/efeitos dos fármacos
Parâmetros de Referência/métodos
Tipo de Publ: Ensaio Clínico Controlado
Estudo de Avaliação
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1047771
Autor: Yan, Xia; Qian, Chaoju; Yin, Xiaoyue; Fan, Xingke; Zhao, Xueyong; Gu, Menghe; Wang, Tao; Ma, Xiao-Fei.
Título: A whole-transcriptome approach to evaluate reference genes for quantitative diurnal gene expression studies under natural field conditions in Tamarix ramosissima leaves
Fonte: Electron. j. biotechnol;35:48-56, sept. 2018. tab, graf.
Idioma: en.
Projeto: National Key Research and Development Program of China; . Natural Science Foundation of China; . Gansu Science and Technology Supporting Project.
Resumo: Background: Tamarix ramosissima is a desert forest tree species that is widely distributed in the drought-stricken areas to sustain the fragile ecosystem. Owing to its wide usage in the desert restoration of Asia, it can be used as an ecophysiological model plant. To obtain reliable and accurate results, a set of reference genes should be screened before gene expression. However, up to date, systematical evaluation of reference genes has not been conducted in T. ramosissima. Results: In this study, we used eigenvalues derived from principal component analysis to identify stable expressed genes from 72,035 unigenes from diurnal transcriptomes under natural field conditions. With combined criteria of read counts above 900 and CV of FPKM below 0.3, a total of 7385 unigenes could be qualified as candidate reference genes in T. ramosissima. By using three statistical algorithm packages, geNorm, NormFinder, and BestKeeper, the stabilities of these novel reference genes were further compared with a panel of traditional reference genes. The expression patterns of three aquaporins (AQPs) suggested that at least UBQ (high expression), EIF4A2 (low expression), and GAPDH (moderate expression) could be qualified as ideal reference genes in both RT-PCR and RNA-seq analysis of T. ramosissima. Conclusions: This work will not only facilitate future studies on gene expression and functional analysis of genetic resources of desert plants but also improve our understanding of the molecular regulation of water transport in this plant, which could provide a new clue to further investigate the drought adaptation mechanism of desert plant species under harsh environments.
Descritores: Tamaricaceae/genética
Transcriptoma
-Padrões de Referência
Adaptação Biológica
Expressão Gênica
Ecossistema
Folhas de Planta/genética
Deserto
Recuperação e Remediação Ambiental
Secas
Reação em Cadeia da Polimerase em Tempo Real
RNA-Seq
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-1278442
Autor: Luo, Hao; Shen, Tie; Xie, Xiaoyao.
Título: The Relationship between Functional and Evolutionary Correlations of Enzyme Reactions in Metabolic Network Evolution
Fonte: Braz. arch. biol. technol;64:e21190480, 2021. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Collaborative Innovation Center of Guizhou Educational Committee; . Science and Technology Foundation of Guizhou Province; . Science and Technology Cooperation Program of Guizhou Province.
Resumo: Abstract The evolution of species is inevitably accompanied by the evolution of metabolic networks to adapt to different environments. The metabolic networks of different species were collected from the Kyoto Encyclopedia of Genes and Genomes (KEGG) website, and some enzyme reactions with the highest occurrence frequency in all species were found and are reported in this paper. The correlation coefficients of whether the enzyme reactions appear in all species were calculated, and the corresponding evolutionary correlation connection networks were calculated according to different correlation coefficient thresholds. These studies show that, as the evolutionary correlation of enzyme reactions increases, the weighted average of the mean functional concentration ratios of the enzyme reactions also increases, indicating that the functional concentration ratio of enzyme reactions has a certain correlation with the evolutionary correlation. The work presented in this paper enhances our understanding of the characteristics and general rules of metabolic network evolution.
Descritores: Ativação Enzimática
Redes e Vias Metabólicas
-Adaptação Biológica
Metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-1103632
Autor: Pinzón-Hernández, Paula Andrea.
Título: Pensar la vida en acción. Una reflexión sobre "Hoy es siempre todavía" / Think life in action. A reflection on "Today is always still"
Fonte: Rev. salud bosque;8(2):111-113, 2018.
Idioma: es.
Descritores: Sobreviventes de Câncer
-Adaptação Biológica
Sobreviventes de Câncer/educação
GENERAL ADAPTATION SYNDROME1
Limites: Humanos
Tipo de Publ: Comentário
Responsável: CO647.9 - Universidad El Bosque


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Id: biblio-839350
Autor: Cardoso, Aline Assis; Andraus, Michel de Paula; Borba, Tereza Cristina de Oliveira; Martin-Didonet, Claudia Cristina Garcia; Ferreira, Enderson Petrônio de Brito.
Título: Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean
Fonte: Braz. j. microbiol;48(1):43-50, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversity and the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of common bean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The isolates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at different temperatures (28 °C, 33 °C, 38 °C, 43 °C and 48 °C). Genotypic characterization was performed based on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbiotic efficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among 98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to temperature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbon sources studied. Clustering analysis based on the ability to use carbon sources and on the tolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropici H12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolates showed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About 20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobium reference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that two efficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercial inoculant for common bean in Brazil and must be assayed in field experiments.
Descritores: Rhizobium/fisiologia
Simbiose
Phaseolus/genética
Phaseolus/microbiologia
Nódulos Radiculares de Plantas/microbiologia
Genótipo
-Filogenia
Rhizobium/isolamento & purificação
Rhizobium/classificação
Adaptação Biológica
Carbono/metabolismo
RNA Ribossômico 16S/genética
Phaseolus/classificação
Meio Ambiente
Tolerância ao Sal
Responsável: BR1.1 - BIREME


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Id: biblio-1018870
Autor: Da Ponte Leguizamón, Natalia.
Título: Dimensión vertical / Dimensión vertical.
Fonte: Asunción; s.e; 2013.Mar. 60 p. ilus.
Idioma: es.
Resumo: La dimensión vertical (DV) es una relación maxilomandibular establecida por la longitud contraída repetitiva de los músculos elevadores. Para su determinación el clínico se encuentra frecuentemente con varias dificultades, una de estas es que a lo largo de los años se han propuesto una gran cantidad de métodos de evaluación y no hay una técnica superior a otra, y depende de cada caso clínico, dado que es diferente en cada paciente. Si la DV se ve alterada, ya sea por aumento o disminución de la DV, pueden generarse problemas fonéticos, de masticación, problemas articulares y estéticos. La pérdida de la DV es un signo clínico y funcional, debemos diagnosticar correctamente ya que existes mecanismos compensatorios que, aunque se pierda la altura dentaria no hay pérdida de dimensión vertical por compensación alveolar. Este tema es muy importante dado que el profesional debe concebir una metodología clara que le permita dominar de forma precisa la DV, sin crear modificaciones que resulten perjudiciales al final del tratamiento. Se considera que es lograr una rutina práctica y fácil ene l control y determinación de la DV nos da grandes beneficios en las rehabilitaciones orales, contribuyendo el manejo clínico exitoso de nuestro tratamiento.
Descritores: Adaptação Biológica/fisiologia
Atrito Dentário
Dimensão Vertical
Odontologia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: PY8.1 - Biblioteca
PY8.1; 617.6, D15d


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Id: biblio-839362
Autor: Park, Gun-Seok; Hong, Sung-Jun; Park, Seulki; Jin, Hyewon; Lee, Sang-Jae; Shin, Jae-Ho; Lee, Han-Seung.
Título: Draft genome sequence of alcohol-tolerant bacteria Pediococcus acidilactici strain K3
Fonte: Braz. j. microbiol;48(1):1-2, Jan.-Mar. 2017.
Idioma: en.
Projeto: Ministry of Education, Science and Technology.
Resumo: Abstract Pediococcus acidilactici strain K3 is an alcohol-tolerant lactic acid bacterium isolated from nuruk, which is a traditional Korean fermentation starter for makgeolli brewing. Draft genome of this strain was approximately 1,991,399 bp (G+C content, 42.1%) with 1525 protein-coding sequences (CDS), of which 44% were assigned to recognized functional genes. This draft genome sequence data of the strain K3 will provide insights into the genetic basis of its alcohol-tolerance.
Descritores: Adaptação Biológica/efeitos dos fármacos
Adaptação Biológica/genética
Genoma Bacteriano
Etanol/farmacologia
Pediococcus acidilactici/efeitos dos fármacos
Pediococcus acidilactici/genética
-Ácido Láctico/biossíntese
Biologia Computacional/métodos
Genômica/métodos
Etanol/metabolismo
Fermentação
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Pediococcus acidilactici/isolamento & purificação
Pediococcus acidilactici/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-1015826
Autor: Vásquez-Ponce, Felipe; Higuera-Llantén, Sebastián; Pavlov, María Soledad; Ramírez-Orellana, Ramón; Marshall, Sergio H; Olivares-Pacheco, Jorge.
Título: Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments
Fonte: Electron. j. biotechnol;28:27-34, July. 2017. tab, ilus, graf.
Idioma: en.
Projeto: Dirección de Investigación, Pontificia Universidad Católica de Valparaíso.
Resumo: Background: In recent years, Antarctica has become a key source of biotechnological resources. Native microorganisms have developed a wide range of survival strategies to adapt to the harsh Antarctic environment, including the formation of biofilms. Alginate is the principal component of the exopolysaccharide matrix in biofilms produced by Pseudomonas, and this component is highly demanded for the production of a wide variety of commercial products. There is a constant search for efficient alginate-producing organisms. Results: In this study, a novel strain of Pseudomonas mandelii isolated from Antarctica was characterized and found to overproduce alginate compared with other good alginate producers such as Pseudomonas aeruginosa and Pseudomonas fluorescens. Alginate production and expression levels of the alginate operon were highest at 4°C. It is probable that this alginate-overproducing phenotype was the result of downregulated MucA, an anti-sigma factor of AlgU. Conclusion: Because biofilm formation is an efficient bacterial strategy to overcome stressful conditions, alginate overproduction might represent the best solution for the successful adaptation of P. mandelii to the extreme temperatures of the Antarctic. Through additional research, it is possible that this novel P. mandelii strain could become an additional source for biotechnological alginate production.
Descritores: Pseudomonas/metabolismo
Alginatos/metabolismo
-Polissacarídeos Bacterianos/metabolismo
Pseudomonas/crescimento & desenvolvimento
Pseudomonas/genética
Adaptação Biológica
Temperatura Baixa
Microscopia Confocal
Biofilmes
Feófitas
Tipagem de Sequências Multilocus
Reação em Cadeia da Polimerase em Tempo Real
Regiões Antárticas
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1000778
Autor: Ricci, Giuseppe; Tonucci, Francesco.
Título: El primer año de nuestro niño / The first year of our child.
Fonte: Buenos Aires; Miño y Dávila; 1988. 216 p.
Idioma: es.
Descritores: Adaptação Biológica
Crescimento
Idioma
Movimento
Ciências da Nutrição
Limites: Recém-Nascido
Lactente
Responsável: AR338.1 - Biblioteca
AR338.1; 610.2, 37, 07/3932



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