Base de dados : LILACS
Pesquisa : G16.500.100 [Categoria DeCS]
Referências encontradas : 5 [refinar]
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Id: biblio-1022816
Autor: Infante, Claudia D; Castillo, Francisca; Pérez, Vilma; Riquelme, Carlos.
Título: Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria
Fonte: Electron. j. biotechnol;33:1-10, May. 2018. graf, tab, ilus.
Idioma: en.
Projeto: CONICYT; . Fondef IDeA; . University of Antofagasta.
Resumo: Background: Marine ecosystems contain benthic microalgae and bacterial species that are capable of secreting extracellular polymeric substances (EPS), suggesting that settlement of these microorganisms can occur on submerged surfaces, a key part of the first stage of biofouling. Currently, anti-fouling treatments that help control this phenomenon involve the use of biocides or antifouling paints that contain heavy metals, which over a long period of exposure can spread to the environment. The bacterium Alteromonas sp. Ni1-LEM has an inhibitory effect on the adhesion of Nitzschia ovalis, an abundant diatom found on submerged surfaces. Results: We evaluated the effect of the bioactive compound secreted by this bacterium on the EPS of biofilms and associated epiphytic bacteria. Three methods of EPS extraction were evaluated to determine the most appropriate and efficient methodology based on the presence of soluble EPS and the total protein and carbohydrate concentrations. Microalgae were cultured with the bacterial compound to evaluate its effect on EPS secretion and variations in its protein and carbohydrate concentrations. An effect of the bacterial supernatant on EPS was observed by assessing biofilm formation and changes in the concentration of proteins and carbohydrates present in the biofilm. Conclusions: These results indicate that a possible mechanism for regulating biofouling could be through alteration of biofilm EPS and alteration of the epiphytic bacterial community associated with the microalga.
Descritores: Diatomáceas
Biofilmes
Microalgas
Matriz Extracelular de Substâncias Poliméricas
-Ambiente Marinho
Incrustação Biológica
Metagenômica
Microbiota
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Costa Rica
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Id: biblio-1003353
Autor: Sánchez-Rodríguez, Diana Elizabeth; Ortiz-Aguirre, Ismael; Aguila-Ramírez, Ruth Noemí; Rico-Virgen, Erika Guadalupe; González-Acosta, Bárbara; Hellio, Claire.
Título: Marine bacteria from the Gulf of California with antimicrofouling activity against colonizing bacteria and microalgae / Bacterias marinas del Golfo de California con actividad antimicrobiana frente a bacterias colonizadoras y microalgas
Fonte: Rev. biol. trop;66(4):1649-1663, oct.-dic. 2018. tab.
Idioma: en.
Resumo: Abstract One way of reducing the input of pollutants into the marine environment is to enforce the use of non-toxic antifouling paints in marine protected areas. Thus, the purpose of this study was to detect marine microorganisms that secrete inhibitory substances against bacteria and microalgae to avoid biofouling on manmade structures in La Paz bay, B.C.S., Mexico. The inhibitory potential of 125 bacteria was evaluated against biofilm-forming bacteria. Crude extracts were obtained with methanol and ethyl acetate from 16 bacterial strains that exhibited antagonistic and antibacterial activity in a preliminary screening. Antibacterial and antimicroalgal assays were performed using crude extracts, the minimum inhibitory concentration (MIC) was determined. The highest activity against bacteria and microalgae was found in two strains, Shewanella algae and Staphylococcus sp. The results of this study suggest that extracts of bacteria from the Gulf of California with antimicrobial properties against biofilm-forming bacteria can also prevent the adhesion of microalgae, which may control the development of biofilm formation and, as a consequence, biofouling.(AU)

Resumen Una alternativa para reducir la contaminación en el medio marino es el uso de pinturas anti-incrustantes no tóxicas en áreas marinas protegidas. En el presente estudio se propuso encontrar microorganismos marinos que secreten sustancias capaces de inhibir la adhesión de bacterias y microalgas, de esta manera evitar la bioincrustación en estructuras marinas en la bahía de La Paz, B.C.S., México. Un total de 125 bacterias fueron evaluadas por su capacidad para inhibir el desarrollo de bacterias formadoras de biopelículas. En una selección preliminar de actividad antagónica y antibacteriana, 16 cepas bacterianas mostraron potencial actividad inhibitoria, de las que se obtuvieron los extractos crudos con metanol y acetato de etilo. Se realizaron ensayos antibacterianos y anti-microalgales utilizando los extractos crudos, se determinó la concentración mínima inhibitoria (MIC). Dos cepas mostraron la mayor actividad contra bacterias y microalgas: Shewanella algae y Staphylococcus sp. Los resultados de este estudio sugieren que los extractos de bacterias aisladas en el Golfo de California que poseen propiedades antimicrobianas contra las bacterias formadoras de biofilm y también pueden prevenir la adhesión de microalgas, con lo que se podría controlar el desarrollo de la formación de biopelículas y como consecuencia, el biofouling.(AU)
Descritores: Poluição do Mar/prevenção & controle
Shewanella
Incrustação Biológica
Microalgas
-California
México
Responsável: BR1.1 - BIREME


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Id: biblio-1116432
Autor: Demirhan, Tanju; Celik, Gokcen Yuvali; Katircioglu, Hikmet; Onbasli, Dilsad.
Título: Evaluation of anti-biofouling potential of Viburnum opulus extracts / Evaluación del potencial anti bioincrustante de extractos de Viburnum opulus
Fonte: Bol. latinoam. Caribe plantas med. aromát;19(3):321-333, mayo 2020. ilus, tab.
Idioma: en.
Projeto: Erciyes University Scientific Research Projects Unit.
Resumo: In this study the in vitro investigation of the inhibitory effect of ethanol extract of Viburnum opulus L. bark sample on Streptococcus mutans planctonic cells and biofilm has been intended. A Scanning electron microscopy analysis has been performed in order to investigate the inhibitory effect of the extract on Streptococcus mutans biofilms. Furthermore, the Exopolysaccharide and dextran production of this bacteria have been identified in the presence of the extract. It has been found out that the bark extract with the concentration of 2,5 mg/mL is able to inhibit more than 50% of the cells in the different times development phases. According to this, the exopolymeric matrix on the biofilm surface disperses and the Exopolysaccharide and dextran production get lowered in the presence of bark extract compared to the control group. It is considered that this extract can be used as an alternative approach for the new chemotherapeutic strategies against tooth decay.

En este estudio se investigó el efecto inhibitorio in vitro del extracto de etanólico de una muestra de corteza de Viburnum opulus L. en biopelículas de células planctónicas de Streptococcus mutans. Se realizó un análisis de microscopía electrónica de barrido para investigar el efecto inhibitorio del extracto sobre las biopelículas de Streptococcus mutans. Además, se identificó la producción de exopolisacárido y dextrano de esta bacteria en presencia del extracto. Se descubrió que el extracto de corteza con una concentración de 2,5 mg/ml inhibió más del 50% de las células en las diferentes fases de desarrollo. Consecuentemente, la matriz exopolimérica en la superficie de la biopelícula se dispersa y la producción de exopolisacárido y dextrano se reduce en presencia de extracto de corteza en comparación con el grupo de control. Se sugiere que este extracto puede ser usado como un enfoque alternativo para las nuevas estrategias quimioterapéuticas contra la carie dental.
Descritores: Streptococcus mutans/efeitos dos fármacos
Extratos Vegetais/farmacologia
Viburnum opulus/farmacologia
Viburnum/química
-Polissacarídeos Bacterianos/análise
Streptococcus mutans/metabolismo
Técnicas In Vitro
Microscopia Eletrônica de Varredura
Dextranos/análise
Biofilmes/efeitos dos fármacos
Etanol
Incrustação Biológica
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1017057
Autor: Rampadarath, Sillma; Bandhoa, Kushlata; Puchooa, Daneshwar; Jeewon, Rajesh; Bal, Subhasisa.
Título: Early bacterial biofilm colonizers in the coastal waters of Mauritius
Fonte: Electron. j. biotechnol;29:13-21, sept. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Mauritius Research Council for funding the project; . LANDRACE.
Resumo: Background: The past years have witnessed a growing number of researches in biofilm forming communities due to their environmental and maritime industrial implications. To gain a better understanding of the early bacterial biofilm community, microfiber nets were used as artificial substrates and incubated for a period of 24 h in Mauritian coastal waters. Next-generation sequencing technologies were employed as a tool for identification of early bacterial communities. Different genes associated with quorum sensing and cell motility were further investigated. Results: Proteobacteria were identified as the predominant bacterial microorganisms in the biofilm within the 24 h incubation, of which members affiliated to Gammaproteobacteria, Alphaproteobacteria and Betaproteobacteria were among the most abundant classes. The biofilm community patterns were also driven by phyla such as Firmicutes, Bacteroidetes, Chloroflexi, Actinobacteria and Verrucomicrobia. The functional analysis based on KEGG classification indicated high activities in carbohydrate, lipid and amino acids metabolism. Different genes encoding for luxI, lasI, agrC, flhA, cheA and cheB showed the involvement of microbial members in quorum sensing and cell motility. Conclusion: This study provides both an insight on the early bacterial biofilm forming community and the genes involved in quorum sensing and bacterial cell motility.
Descritores: Água do Mar/microbiologia
Bactérias/crescimento & desenvolvimento
Bactérias/genética
Fenômenos Fisiológicos Bacterianos
-Bactérias/isolamento & purificação
Bactérias/classificação
Aderência Bacteriana
Movimento Celular
Biofilmes
Biodiversidade
Percepção de Quorum
Incrustação Biológica
Metagenômica
Sequenciamento de Nucleotídeos em Larga Escala
Maurício
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-768242
Autor: Fujita, D. S.; Takeda, A. M.; Coutinho, R.; Fernandes, F. C..
Título: Influence of antifouling paint on freshwater invertebrates (Mytilidae, Chironomidae and Naididae): density, richness and composition / Influência de revestimentos antiincrustantes em invertebrados aquáticos (Mytilidae, Chironomidae and Naididae): densidade, riqueza e composição
Fonte: Braz. j. biol;75(4,supl.1):70-78, Nov. 2015. tab, graf.
Idioma: en.
Projeto: CTHIDRO/CNPq.
Resumo: Abstract We conducted a study about invertebrates on artificial substrates with different antifouling paints in order to answer the following questions 1) is there lower accumulation of organic matter on substrates with antifouling paints, 2) is invertebrate colonization influenced by the release of biocides from antifouling paints, 3) is the colonization of aquatic invertebrates positively influenced by the material accumulated upon the substrate surface and 4) is the assemblage composition of invertebrates similar among the different antifouling paints? To answer these questions, four structures were installed in the Baía River in February 1st, 2007. Each structure was composed of 7 wood boards: 5 boards painted with each type of antifouling paints (T1, T2, T3, T4 and T5), one painted only with the primer (Pr) and the other without any paint (Cn). After 365 days, we observed a greater accumulation of organic matter in the substrates with T2 and T3 paint coatings. Limnoperna fortunei was recorded in all tested paints, with higher densities in the control, primer, T2 and T3. The colonization of Chironomidae and Naididae on the substrate was positively influenced by L. fortunei density. The non-metric multidimensional scaling (NMDS) of the invertebrate community provided evidence of the clear distinction of invertebrate assemblages among the paints. Paints T2 and T3 were the most similar to the control and primer. Our results suggest that antifouling paints applied on substrates hinder invertebrate colonization by decreasing the density and richness of invertebrates.

Resumo O estudo dos invertebrados em substrato artificial com diferentes revestimentos antiincrustantes foi realizado com o intuito de responder as seguintes questões 1) Há menor acumulação de material orgânico nos substratos com revestimentos antiincrustantes? 2) A colonização de invertebrados é afetada pela liberação de biocidas dos revestimentos antiincrustantes? 3) A colonização dos invertebrados aquáticos é facilitada por material acumulado sobre a superfície do substrato? 4) A composição da assembléia é similar entre os diferentes revestimentos? Para realização deste estudo, quatro estruturas foram instaladas em 01 de fevereiro de 2007 no rio Baía. Em cada estrutura foram colocadas sete placas de madeira: cinco placas com aplicação de um tipo de revestimentos antiincrustantes (T1, T2, T3, T4 e T5), uma placa apenas com a aplicação do primer (Pr) e a outra placa permaneceu sem aplicação de nenhum revestimento (Cn). Ao final dos 365 dias em que os substratos ficaram submersos observou-se maior acumulação de material orgânico nos substratos dos revestimentos T2 e T3. Limnoperna fortunei foi registrada em todos os revestimentos testados, com maiores densidades encontradas no controle, primer, T2 e T3. A colonização de Chironomidae e Naididae sobre os substratos foi influenciada pela densidade de L. fortunei. A ordenação (NMDS) evidenciou a separação da assembléia de invertebrados entre os revestimentos. Os revestimentos T2 e T3 foram os mais similares ao controle e ao primer. Os resultados obtidos sugerem que revestimentos antiicrustantes aplicados sobre substratos dificultam a colonização de invertebrados, reduzindo a densidade e riqueza de invertebrados.
Descritores: Chironomidae/efeitos dos fármacos
Mytilidae/efeitos dos fármacos
Oligoquetos/efeitos dos fármacos
Pintura/toxicidade
Praguicidas/toxicidade
Poluentes Químicos da Água/toxicidade
-Biodiversidade
Brasil
Incrustação Biológica/prevenção & controle
Densidade Demográfica
Rios
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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